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Firstly we prepared cup culture of hydra to increase the number of hydra, so that we can study regeneration in hydra. For preparation of cup culture of hydra we took six cups and we added 50ml of DC water in each cup and added one-one hydra in each cups. To feed hydra we added moina we added 20-22 moina in each cups. After increase in the number of hydra we amputed six hydras. 1 setup :Head + body part and basal +body part.Observation after 44 hrs : Head part regenerated , basal part regenerated small tentacles were seen. 1 setup duplicate Head +bodypart and basal+ bodypart .Observation after44 hrs : We didn't observe regeneration. 2 setup: Head part, middle part and basal part.Observation after 44 hrs: We didn't observe regeneration.
2 setup duplicate :Head part,middle part and basal part. Observation after 44 hrs : Head part regenerated,middle part regenerated and basal part regenerated small tentacles were seen.3 setup :only tentacles,basal disc and remaining part of hydra .Observation after 44 hrs :We didn't observe regeneration.3 setup duplicate : only tenatcles , basal disc and remaining part of hydra. Observation after 44 hrs:Remaining part of hydra regenerated.
Today on 6/1/18 we have 64 hydras. Cup culture of hydra made on 26/12/17.
 
License:[Source ] Aug. 6, 2019, noon


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