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CUBE Winter Meet, 11th February 2018.
HBCSE, TIFR, Mumbai.
 
Many Inspired minds discussed about the simple yet elegant model systems. How beautiful questions can be addressed and logical hypothesis can be drawn out of fascinating observations!! Importance of science in everyday life! How 'Citizen Science' is spreading and people are being a part of collaboratively understanding seasonomics! Mango mapping, bee mapping, butterfly mapping etc. many new and emerging model systems in CUBE were also discussed which needs more attention and daily reporting and discussion of the observations by the respective Cubists like the Cardamine, spiders and butterfly systems. during the discussions it was realized that we need to carry forward the work that all cubists are doing and step ahead to ask some more brilliant questions and not only repeat what has been already done previously by other cubists. It is good to start with the previous experiments/datasets but all of us realized that a lot of science with every model system is untouched as we fail to look upon that aspect of our data. Overall it was a good day to celebrate science a day before 'Darwin Day’!! Cheers to all!!
 
Here are some of the important questions and points that were raised during discussion while various groups presented. I would like everyone to read and ponder upon these points. Let’s do things more robustly and systematically and have an even better discussion in the next meet. 
 
The hydra groups.
 
·Why not putting a control along with the test sample during experiments? 
It is equally important to put controls and show them also while presenting.
·What is the fundamental reason behind various set-ups like hydra+ algae, hydra+ algae+ moina and hydra alone in water? Explaining this helps both the audience and yourself to understand the hypothesis better.
·Why feeding hydra with moina, but not bacteria directly? Can it be done? Why or why not?
·What about the growth pattern of hydra during different conditions?
We can also look for the growth pattern also in terms of size and number of tentacles etc. along with the number of hydra surviving.
 
The earthworm groups-
 
·Can we work on some alternate way of finding earthworm from soil instead of using soap water? As it was discussed it may deter the soil quality. Nagarjun sir suggested using damp thick cardboard paper and keep it on soil and check after 2-3 days. Earthworms can be collected from beneath it. Let’s try this!!
·What about the smaller segments survival in regeneration experiments? Why we are neglecting this part and not appreciating the beautiful science happening in these segments.
·And also the bigger segments which are reported to survive up to 94 days without head!!  Worm without head! What is happening here? How survival is possible without head? 
·Regular observation and recording with pictorial evidences should be done.
 
The drosophila groups-
 
·Can we distinguish between males and females while studying their activity pattern for the day and see if there is any difference? Why or why not?
·Drosophila as model system for studying evolution!! How Olfaction can be used as a parameter to understand evolution!
·Native flies vs csbz flies for olfactory assays? What is special with the csbz flies?
What is the basis of the hypothesis that native flies are better? What are the other lines that you will check?
·How to identify that a particular fly belongs to the genus Drosophila and specie melanogaster?
·What may be the role of mutation in making csbz less evolved In terms of olfactory abilities as compared to native ones? Can it be simple habituation over generations and not mutation in case of csbz. 
 
A suggestion-
·Use ice to anesthetize the flies to select/pick any one or more flies from the bottle/vial culture for the experiments.
 
 
The moina groups-

·How is hemoglobin gene regulated in moina.
·How the hypoxic condition affect the baby moina which is still inside mother moina?
·Experiments should be planned with proper controls as this time the presentations were lacking pictures of proper controls.
·Try to choose moinas of same age and size to do comparative studies of any kind or pictorial data.
 
The snail groups-
 
·The sample size for the data was very less for the behavioral and conditioning assay.
Why not using all the snails you have and that also multiple times for the robust data.
·The experimental design was not good. How about making a flow chart for the design and share and discuss of various groups before proceeding with it. (You can simply draw the flow chart on a paper and share a pic.)
 
The Pagalapos group-
 
·Horizontal gene transfer (HGT) leads to incorporation of a large amount of DNA in the progeny genomes over a large number of generation. How it affected its evolution and genome size of rotifers?
·The tuns (desiccated cysts) didn’t revived when desiccated in a 96 well plates without filter paper at the bottom. Why? Try finding an answer to it.
·How big are these cysts? Can they be observed under a simple scope/ magnifying glass?
If yes, can you check whether all the tuns got carried away by air or there is some other reason for them not reviving?
 
The mosquito group-
 
·With the help of the new method of ovitrap can we now make a systematic plan to look for various eggs of different mosquito species in different seasons?
·Let’s do this for clear as well as dirty water to see the trend.
·Also let’s try getting BMC data and superimpose on yours to see what your results are like?
 
The cardamine group
·What questions can be better addressed in cardamine but not in Arabidopsis as it is more complex than Arabidopsis?
·What is the pattern of leaf morphology in cardamine plant as we move from basal region to above? Does it differ from plant to plant?
·What is the genetic basis of different leaf morphology in the cardamine plant? Are there any studies regarding it? Please read and discuss the reference in respective groups.
·How are you planning to transfer your gene of interest and what results you expect? What is the basis of your hypothesis?
·Please read, share and discuss some literature on these topics.
 
Let’s meet next time with the answers backed up by experimental data and literature. Till then keep observing, recording, reporting and discussing. CHEERS !!
Regards
Rahul.
 
License:[Source ] Oct. 26, 2019, 9:39 a.m.

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what is aim of the mosquito project?
by-harshal kedare
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