Biological Rhythm Studies in Fruit Flies

Today’s study evolution

How to prepare agar?


Preparation of agar

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Procedure

  1. Measure agar and distilled water into clean flask or beaker Agar + Distilled Water = Yield

  2. Flame sterilize a clean glass stir rod to stir the medium as it melts.

  3. While wearing heat resistant hand protection, hold the flask or beaker over the flame. Swish or stir the mixture constantly while heating.

  4. Boil the mixture for 1 minute. Remove from heat.

  5. Place a sterile lab thermometer. in the mixture and monitor the temperature until it falls to approximately 45 - 50° C or if a lab thermometer is not available, cover and let stand a few minutes.

  6. Pour enough melted agar into each sterile plastic petri dish to cover the bottom - about 1/8" to 1/4" deep. Replace the lid immediately.

  7. Place agar plates on a counter top to cool and set. Agar medium will set like stiff gelatin at room temperature.

  8. The agar medium is now ready for storage or use.

Storage : Stack agar plates upside down in the refrigerator. Do Not Freeze! The purpose of placing the plates upside down is to prevent condensation from dripping down onto the agar surface which could then facilitate movement of organisms between colonies.

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Objective: studying evolution.
We studied about behavioural changes of fruit flies like sense of smell.
Lab bred flies have a weaker sense of smell as compared to the ones which breed naturally in the environment. Lab bred are provided with favourable conditions whereas the one breeding naturally have to struggle for nutrition.

We will also prepare a source of food for fruit flies made of agar and then observe the movement of larva towards the source of food made of agar and distilled water.
We must ensure that we do not use nutritional agar since it has many nutrients like peptones

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Objective: studying evolution.
We studied about behavioural changes of fruit flies like sense of smell.
Lab bred flies have a weaker sense of smell as compared to the ones which breed naturally in the environment. Lab bred are provided with favourable conditions whereas the one breeding naturally have to struggle for nutrition.

We will also prepare a source of food for fruit flies made of agar and then observe the movement of larva towards the source of food made of agar and distilled water.

1 Like

Objective: studying evolution.
We studied about behavioural changes of fruit flies like sense of smell.
Lab bred flies have a weaker sense of smell as compared to the ones which breed naturally in the environment. Lab bred are provided with favourable conditions whereas the one breeding naturally have to struggle for nutrition.

We will also prepare a source of food for fruit flies made of agar and then observe the movement of larva towards the source of food made of agar and distilled water.

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Procedure:

  1. Measure agar and distilled water into clean flask or beaker.

Recipe: Agar + Distilled Water = Yield

Agar
23 g
11.5 g
9.2 g
4.6 g + Distilled Water
1000 ml
500 ml
400 ml
200 ml = Yield
50 plates
25 plates
20 plates
10 plates

  1. Flame sterilize a clean glass stir rod to stir the medium as it melts.
  2. While wearing heat resistant hand protection, hold the flask or beaker over the flame. Swish or stir the mixture constantly while heating.
  3. Boil the mixture for 1 minute. Remove from heat.
  4. Place a sterile lab thermometer. in the mixture and monitor the temperature until it falls to approximately 45 - 50° C or if a lab thermometer is not available, cover and let stand a few minutes.
  5. Pour enough melted agar into each sterile plastic petri dish to cover the bottom - about 1/8" to 1/4" deep. Replace the lid immediately.
  6. Place agar plates on a counter top to cool and set. Agar medium will set like stiff gelatin at room temperature.
  7. The agar medium is now ready for storage or use.
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You can be a lil more original instead of copy pasting my content :upside_down_face:

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We tried our best but
Ohho we failed :no_mouth::blush:

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Ohhh … it’s such a coincidence that we think alike… it’s crazy :upside_down_face:

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Oof, nevermind. Might want to exercise your brain a little next time☺️

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Ohhh sorry…i thought great minds think alike :smiley:

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What type of chat is going on the group :joy:

We studied how to make agar agar

“Nothing in Biology Makes Sense Except in the Light of Evolution”

We have studied that the flies breeding in the lab have a weaker sense of smell when compared with the flies breeding in the environment. This is because the flies breeding in the lab are being provided with good conditions and food whereas the flies breeding in the environment have to struggle to find food for their survival and they gradually develop stronger smelling senses

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Procedure:

  1. Measure agar and distilled water into clean flask or beaker.

Recipe: Agar + Distilled Water = Yield

Agar
23 g
11.5 g
9.2 g
4.6 g + Distilled Water
1000 ml
500 ml
400 ml
200 ml = Yield
50 plates
25 plates
20 plates
10 plates

  1. Flame sterilize a clean glass stir rod to stir the medium as it melts.
  2. While wearing heat resistant hand protection, hold the flask or beaker over the flame. Swish or stir the mixture constantly while heating.
  3. Boil the mixture for 1 minute. Remove from heat.
  4. Place a sterile lab thermometer. in the mixture and monitor the temperature until it falls to approximately 45 - 50° C or if a lab thermometer is not available, cover and let stand a few minutes.
  5. Pour enough melted agar into each sterile plastic petri dish to cover the bottom - about 1/8" to 1/4" deep. Replace the lid immediately.
  6. Place agar plates on a counter top to cool and set. Agar medium will set like stiff gelatin at room temperature.
  7. The agar medium is now ready for storage or use.
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Preparation of Petri Dish with Agar

Before starting, ensure that the Petri dish (dishes) is closed/has its lid on until you are ready to pour the agar in to them.

Requirements

  • Agar powder
  • Lab thermometer
  • Distilled water
  • Glass stir rod
  • Heat resistant hand protection
  • Boiling mixture
  • Sterile Petri dish
  • Beaker/flask

Procedure

  1. Measure the recommended amount of agar and distilled water in to a clean, sterile flask or beaker
  2. Using heat resistant hand protection, hold the beaker/flask over a flame and stir the mixture gently using a sterile stir rod while heating
  3. Continue boiling the mixture for about one minute, and then remove from heat
  4. Place a sterile lab thermometer in the mixture and monitor until its temperature falls to about 47 degrees (45- 50 degrees)
  5. Pour melted agar in to the Petri dish to cover the bottom (about a quarter) and replace the lid immediately
  6. Allow the agar plate to cool and set (the medium will set like gelatin at room temperature)
  7. It is ready for storage once it sets
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Now we are going to prepare plain agar solution as a medium