# Celebrating Goof ups and Feeling for the Organism!

**URL:** https://metastudio.org/t/celebrating-goof-ups-and-feeling-for-the-organism/15615
**Category:** CUBE chat
**Created:** [April 29, 2025, 5:23am UTC](https://metastudio.org/t/celebrating-goof-ups-and-feeling-for-the-organism/15615 "2025-04-29T05:23:26Z")
**Posts on this page:** 1
**Showing post:** 2

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### Author: ![Ayana\_Sudheer](https://metastudio.org/user_avatar/metastudio.org/ayana_sudheer/32/18425_2.png) [@Ayana\_Sudheer](https://metastudio.org/u/Ayana_Sudheer)
#### Post date: [April 29, 2025, 6:00am UTC](https://metastudio.org/t/celebrating-goof-ups-and-feeling-for-the-organism/15615/2 "2025-04-29T06:00:36Z")

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**Learning from my mistakes, I tried making TRSV media again on 28/04/25 at my CUBE homelab, Nallalam, Kozhikode**  
@magpie used to share pictures of her fruit fly culture and also has shared about the TRSV media. That inspired me to culture fruitflies at my homelab also!  
[28/04, 9:06 am] ayanasudheer: @⁨~Himanshu Joshi⁩ @⁨Batul Pipewala⁩ @⁨Theertha Cube⁩  
How to transfer fruit flies from one bottle to another.? Pls suggest.  
[28/04, 11:03 am] Theertha Cube: Can we place another bottle over it and tap the below bottle.  
[28/04, 11:04 am] Theertha Cube: How to trap fruitflies, written by Aswathy _Trapping Fruit Flies_

> [@Fruit flies study in home lab](https://metastudio.org/t/fruit-flies-study-in-home-lab/4675/7):
>
> Trapping Fruit Flies We can trapp fruit flies Using Fruit / Rotten Baits . Bait are 2 type Open bait or Closed bait. Both baits are shows different .in the case of open bait we kept a fruit any kind of paper for attracting flies and in the case of closed bait , fruits are kept in the bottle and invited flies after flies were in to the bottle just closed the mouth of bottle that way we trap flies and culture them safely . In open Bait ( Tomato / Banana ) we can trap flie…

> [@Fruit flies study in home lab](https://metastudio.org/t/fruit-flies-study-in-home-lab/4675/3):
>
> TRSV - STANDARDIZED Culturing Fruit Flies At Home Lab INGREDIENTS 1 egg size Tomato 2 table spoon Rava 1 table spoon Sugar ~150 mL Water (Sanitised bottle) ~15 mL Vinegar (medicine cap) PREPARATION Taken a pan turn on flame added ~150ml water ,2 table spoon rava ,1 spoon sugar ,1egg sized tomato smashed . Continually mixed mixture at 10 minutes low flame . Off flame and 5 minutes leave it for cooling .final added ~15ml Vinegar in to whole medium and mixed it well…

Following the standardized protocol posted by the cubist @saswathy679, I made TRSV media again  
**Ingredients needed**  
**1 egg sized tomato**  
**2 tbspoons of Rava**  
**1 tbspoon of Sugar**  
**150ml water**  
**5ml vinegar**

Procedure:  
Add 150ml of water in a pan. Keep it it very low flame. Add 1 tbs of Rava and sugar to the pan. Stir it continuously for 2 minutes. Then add the smashed tomato to the pan. Cook for 10 minutes in low flame. Stir continuously. Then, allow it to cool for 5 minutes. Now TRSV will be thick. By adding 5ml of Vinegar, it will become slight liquidy.

 ![IMG_20250429_112548](https://metastudio.org/uploads/default/original/3X/b/4/b4c782f7bd395e21fe21855630dc1a755b5c0ab3.jpeg)  
TRSV is transferred soon into two bottles (250ml, 600ml bottles cleaned and dried in sun) using a funnel without touching the walls.  
 ![IMG_20250429_112805](https://metastudio.org/uploads/default/original/3X/d/f/df9e7cc5820b45eb514a030d4a3e13c99adc4cb8.jpeg)

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