A glimpse of the CUBE ChatShaala-Tuesday, 25th August 2020
The introductory slide of yesterday’s discussion in CUBE ChatShaala.
The bright heading describes the idea to locate and trap fruit flies. Trapping fruit flies may seem an easy task to do, but there are little details which need to be looked upon when we do the experiment so that we will reach our objective steadily. We have parameters such as the location of the trap, condition of the attractant/bait and most importantly, the anticipation from our work.
Here, we are designing an experiment in order to trap fruit flies in our home labs. In this image from the whiteboard, firstly we see that a hypothesis has been made here (a good start!) which is followed by details of the bait having replicates, the location and at a particular time, the bait is being kept. In the previous photograph, we had seen the expectations from the setup.
An update from one of the CUBE home labs in Kerala. In Kerala, we have two home labs having Single Line Cultures (SLCs) of fruit flies. Here, we have BRSV (Banana Rava Sugar Vinegar) medium being used to culture fruitflies in bottles. Taking inspiration from the CUBE Fraternity, we have names for these SLCs. They are SLBK (Single Line Bottukadavu) and SLTT (Single Line Thanniam, Thrissur). And we have two SLCs in Mumbai too. It would be good if the respective CUBists name them!
Citing some difficulties in the maintenance of fruit flies (most probably, culture medium issue), CUBists had made improvisations in the TRSV (Tomato Rava Sugar Vinegar) medium.
Can we list them?
Following the update, we also learned how to define a good culture medium?
A culture bottle (with a medium) having larvae, unhatched/un-ecloded pupae and flies, all three of them together falls under the criteria of a good culture bottle with a reproducible result.
What are your thoughts, folks?
Credits for the Images (all): @KiranyadavR