Whiteboard screenshot( 10th Dec 2020 ) CUBE CHATSHAALA.
What’s the journey that we have traveled so far since the lockdown was announced.
TRSV CULTURE MEDIUM
One of the achievement we got during lockdown.
What are the oppertunities that lockdown has given us and we could able to innovate new ideas and had also innovate TRSV culture medium that had helped us in culturing Native trapped fruit flies… and still able to do it’s work.
We had discussed the whole story that how TRSV medium was made. What all goof ups we did , what modifications we made in order to get standard TRSV CULTURE MEDIUM .
@Aswathy Cube Butterfly and fruit fly collaborators have been maintaining single line cultures of trapped native fruit flies using TRSV CULTURE MEDIUM and few techniques
We had whole story of CUBE HOME LAB BOTTUKAVADU KERALA in today’s webinar.
And for a reason @Aswathy Cube Butterfly had to leave cultures for sometimes and how did @Aswathy Cube Butterfly managed to save all the single line cultures that she was maintaining.
Fruit fly collaborators please help Aswathy in order to save this bottleneck.
Aswathy once left with SLBK-A8, B8, C8 & E4 culture bottles.
But Aswathy has managed to save them by transferring flies on 3/12/2020 from A8,B8, C8 &E4 to new media bottles A9, B9, C9 & E5 respectively…
But after doing this attempt Aswathy is not getting good results in A9 and C9 bottle
Following are the conditions of these bottle👇🏻
A9: 5 Larvae & 1 Pupa
C9: 3Larvae & No pupa
Please help Aswathy in order to save precious Cultures.
Aswathy please give details of your immediate plans.
we also had Discussion on how does soap works?. As @Aswathy Cube Butterfly had lost SLBK-D single line culture we as per @Aswathy Cube Butterfly dialogue we could say that bottles were not washed well so due to insufficient washing detergent must have left .
So we need to discuss how does soap works?.
We have been trying to standardized the method of making TRSV CULTURE MEDIUM so that we’ll be having reproducible results.
So can we also standardized the method of transfer of fruit flies from Old media bottle to new media bottle
We had a very nice discussion where @Aswathy Cube Butterfly tried to standardized the method of transfer.
How many flies we should Transfer in order to make subcultures?..
After how many days we should Transfer?..