S3E32 Cube chatShaala: Exploring new ways of Learning

Meeting URL

webinar.hbcse.tifr.res.in

HBCSE Webinar

A front-end for HBCSE web conferencing server. You can create your own rooms to host sessions, or join others using a short and convenient link.

Please click on the HBCSE Webinar above to join the CUBE ChatShaala
Timing:5.30 pm to 9.30 pm
Webinar will be recorded and recording link will be post in the same thread after the webinar.

Looking forward to your participation.
Thanks

1 Like

CUBE ChatShaala on 10th December 2020
37 CUBists from 34 Centres had joined the webinar.
Map

INDIA

ANDHRA PRADESH:
Visakhapatnam: Sanya
Guntur: Suma Swarajya Lakshmi
Nellore: Ujwalaraj

ASSAM:
Sonari: Susanta Tanti

BIHAR:
Sitamarhi: Anamika Singh
Patna: Kshipra

DELHI:
AND College: Aastha Ahuja, Dr Sarita Kumar
DDU College: Dr Sudhir Verma

JHARKHAND:
Ranchi: GC Baskey

KERALA:
Ernakulam: Ashikha Farzana, Essabell Philomina, Rexeena Sebastian
Kozhikode: Arunima
Thriprayar, Thrissur: Aswathy Suresh
Kandassankadavu: Lakshmy PJ
Thanniam, Thrissur: Sidhy PP
Perumbavoor: Theres Hemin

MADHYA PRADESH:
Gwalior: Komal Singh

MAHARASHTRA:
South Bombay: Arunan MC
Ghatkopar, Mumbai: Aashutosh Mule
Thane: Anshu Kadam
Adarsha Vidyalaya, Chembur: Dhanashree Chandankar
Nerul: Drishtant M Kawale
Powai: Lydia Mathew
Nagpur: Riya Soitkar
Dahanu, Palghar: Sachin Pradhan
Bandra, Mumbai: Saida Sayyed
Murbad, Kalyan: Shubham Isame

TAMIL NADU:
Chennai: Anjali Chauhan

UTTAR PRADESH:
Kanpur: Hina Mudgal
Moradabad: Kiran Yadav

WEST BENGAL:
Kolkata: Batul Pipewala

Bhavika
Nashra Mubeen
Rachna Gwolikar

THE UNITED STATES OF AMERICA
Anil Challa

IMG_20210102_145209
Whiteboard screenshot( 10th Dec 2020 ) CUBE CHATSHAALA.

What’s the journey that we have traveled so far since the lockdown was announced.

TRSV CULTURE MEDIUM
One of the achievement we got during lockdown.
What are the oppertunities that lockdown has given us and we could able to innovate new ideas and had also innovate TRSV culture medium that had helped us in culturing Native trapped fruit flies
 and still able to do it’s work.

We had discussed the whole story that how TRSV medium was made. What all goof ups we did , what modifications we made in order to get standard TRSV CULTURE MEDIUM .
IMG_20210102_145226
@⁚Aswathy Cube Butterfly⁩ and fruit fly collaborators have been maintaining single line cultures of trapped native fruit flies using TRSV CULTURE MEDIUM and few techniques

We had whole story of CUBE HOME LAB BOTTUKAVADU KERALA in today’s webinar.

And for a reason @⁚Aswathy Cube Butterfly⁩ had to leave cultures for sometimes and how did @⁚Aswathy Cube Butterfly⁩ managed to save all the single line cultures that she was maintaining.

Fruit fly collaborators please help Aswathy in order to save this bottleneck.
IMG_20210102_145245
Aswathy once left with SLBK-A8, B8, C8 & E4 culture bottles.

But Aswathy has managed to save them by transferring flies on 3/12/2020 from A8,B8, C8 &E4 to new media bottles A9, B9, C9 & E5 respectively


But after doing this attempt Aswathy is not getting good results in A9 and C9 bottle
Following are the conditions of these bottle👇🏻
A9: 5 Larvae & 1 Pupa
C9: 3Larvae & No pupa

Please help Aswathy in order to save precious Cultures.

Aswathy please give details of your immediate plans.

IMG_20210102_145300
we also had Discussion on how does soap works?. As @⁚Aswathy Cube Butterfly⁩ had lost SLBK-D single line culture we as per @⁚Aswathy Cube Butterfly⁩ dialogue we could say that bottles were not washed well so due to insufficient washing detergent must have left .

So we need to discuss how does soap works?.
IMG_20210102_145638
We have been trying to standardized the method of making TRSV CULTURE MEDIUM so that we’ll be having reproducible results.

So can we also standardized the method of transfer of fruit flies from Old media bottle to new media bottle

We had a very nice discussion where @⁚Aswathy Cube Butterfly⁩ tried to standardized the method of transfer.

  1. How many flies we should Transfer in order to make subcultures?..

  2. After how many days we should Transfer?..