S4E37 CUBE chatShaala: Understanding Mutation

Drosophila and SARS-COV2 as context to understand Mutation, @saswathy679 has been successful in maintaining five single line cultures of fruit flies in her CUBE Home Lab Bottukadavu Kerala, the long term objective is to compare olfaction sensitivity in between trapped native fruit flies single line cultures and identified CsBz strain of Drosophila melanogaster but why we need to compare the olfaction is there any kind of mutation taking place?..

HBCSE Webinar

Please click on the HBCSE Webinar above to join the CUBE ChatShaala
Timing:5.30 pm to 9.30 pm

The webinar will be recorded and the link of the recordings can be found here: HBCSE Webinar (tifr.res.in)
Discussion will be followed by Nail regeneration workshop by 8.30pm onwards.
Looking forward to your participation.

405th day of CUBE ChatShaala: 28th April 2021
24 CUBists from 23 Centres had joined the webinar

Guntur: Suma Swarajya Lakshmi

Narsing High School, Silchar: Krishnendu Roy

Morabadi, Ranchi: Rechel Tirkey

Kozhikode: Arunima
Thriprayar, Thrissur: Aswathy Suresh
CDAC, Thiruvananthapuram: Hemant Jeevan Magadum, Mani CS
Palakkad: Dr Nisy Prasad
SN College, Nattika: Seethalakshmi CR
Thanniam, Thrissur: Sidhy PP
Alapad, Thrissur: Vishnupriya

Thane: Anshu Kadam
South Bombay: Arunan MC
Nerul: Drishtant M Kawale
HBCSE, Mumbai: DP
Worli, Mumbai: Isha Pawle
Mulund, Mumbai: Priti Kanade
Kalyan: Shivam Kumar Sriwas
Panvel, Navi Mumbai: Shraddha Sonavane
MVLU College, Mumbai: Urvi Upadhyay

Nawalgarh: Hitesh Pareek

Moradabad: Kiran Yadav

Kolkata: Batul Pipewala

Kadar AA

i) A request to the above-unaffiliated CUBist to share their affiliation in the CUBE Groups wherein they receive this message. It needs to be updated in CUBE Records.
ii) CUBist(s) who had joined today but their name isn’t included in the list, kindly reply to this message.





Above are the whiteboards of today’s (28th April 2021) CUBE ChatShaala

As you’ll be able to make out from the interesting drawings on the board, the discussion revolved around Molecular Biology, today.

i) Starting with how to extract DNA using the minimalistic things available at home, supplementing for SDS, Ethanol, etc. used in laboratories.
ii) The first photo depicts the procedure to extract DNA. Followed by the structure and composition of a cell of the fruit :banana: Banana.
iii) This led to a cascade of questions such as
a) Why is :banana: being used?
b) Why is Shampoo used?
c) Where is DNA (hidden) in the Banana?
d) Is there really DNA in Banana? If yes, how can we visualise it?
iv) Meanwhile, the effect of detergent, salt on the cell was looked upon.
You can refer to the detailed drawing of the hydrophobic tail and hydrophilic head depicted using different colours.
v) Next, was the discussion on the heart of Molecular Biology viz. The Central Dogma
vi) How exactly is a mere strand of DNA converted (transcribed) to mRNA and then translated to a 3D Protein?
vii) All of this involves, a plethora of enzymes and processes such as Intron Splicing, Capping, etc.
viii) If introns are cut in the splicing part, why at all are they present in the genome?
In both Prokaryotes and Eukaryotes alike?
ix) You can make out in the right side of the third picture, CRISPR that stands for Clustered Regularly Interspaced Short Palindromic Repeats (such a long name😬) often regarded as the immune system of prokaryotes especially bacteria.
x) CRISPR is a very essential discovery made by TWO WOMEN SCIENTISTS, Jennifer Doudna and Emmanuelle Charpentier, for which they were awarded the 2020 Nobel Prize in Chemistry (for work in Molecular Biology​:flushed::100:)! This discovery shouldn’t go without mention as it involves WOMEN SCIENTISTS received NOBEL PRIZE!
xi) This was eventually followed by the Nail Session wherein a short introduction to new participants was given furthermore, their inputs were taken and a general hypothesis w.r.t. the growth of the Nail was proposed.
Nail Growth is Faster in young people than older ones and it further varies in Females (premenopausal and menopausal) and Males.
xii) A graph has also been plotted.

Do catchup on it and ask questions for clarification and continuation of the discussion!