Understanding the Genetic Diversity Study, by Using RAPD Techniques Among varieties of Mangoes

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Summary of 14/05/2022 Chatshaala☘️

:shamrock:We started discussion with Mango mapping .

:shamrock:Also discussed with the different varieties of mangoes and their local names .

:shamrock:Then we disscussed about the Article send by @⁨Arunan Sir⁩ .That is about the RAPD analysis of different mango varieties of a thaluk in Thiruvananthapuram ,kerala .

:shamrock:Then we come across the term RAPD ,what is RAPD ,is this the same of PCR!!!

:shamrock:Then we started to discussion on PCR ,Polymerase chain reaction .

:shamrock:PCR is a technique used to amplify the DNA.In one cycle we get 2 sequence DNA.

:shamrock:first the double stranded DNA seperated in to two by breaking the H bonds using specific heat 95°c.

:shamrock:then we got two seperate strands,Strand 1 and strand 2 .
:shamrock:Then we add Primers to the 3 ’ end of the strand 1,that is complementary to strand 1,cooling at 54 °c.
:shamrock:with the help of DNA polymerase corresponding complementary strands are formed .

:shamrock: @⁨Sunitha Mam⁩ mentioned about that here that is Taq polymerase .

Questions to be disscussed :
:maple_leaf:why PCR is used ,if we got 2 DNA strand from one cycle what is the purpose of it !?
:maple_leaf:what is the term indicate, Polymerase chain reaction !?
:maple_leaf:what the process occur in RTPCR !?
:maple_leaf:Why Taq polymerase is used here !?
:maple_leaf:When compare to PCR ,what is RAPD!?

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So how is PCR connected with RAPD? And how these two are connected with variety of Mango?

Why do you think RAPD and PCR is the same? any reference?