Summary of 14/05/2022 Chatshaala☘️
We started discussion with Mango mapping .
Also discussed with the different varieties of mangoes and their local names .
Then we disscussed about the Article send by @Arunan Sir .That is about the RAPD analysis of different mango varieties of a thaluk in Thiruvananthapuram ,kerala .
Then we come across the term RAPD ,what is RAPD ,is this the same of PCR!!!
Then we started to discussion on PCR ,Polymerase chain reaction .
PCR is a technique used to amplify the DNA.In one cycle we get 2 sequence DNA.
first the double stranded DNA seperated in to two by breaking the H bonds using specific heat 95°c.
then we got two seperate strands,Strand 1 and strand 2 .
Then we add Primers to the 3 ’ end of the strand 1,that is complementary to strand 1,cooling at 54 °c.
with the help of DNA polymerase corresponding complementary strands are formed .
@Sunitha Mam mentioned about that here that is Taq polymerase .
Questions to be disscussed :
why PCR is used ,if we got 2 DNA strand from one cycle what is the purpose of it !?
what is the term indicate, Polymerase chain reaction !?
what the process occur in RTPCR !?
Why Taq polymerase is used here !?
When compare to PCR ,what is RAPD!?
So how is PCR connected with RAPD? And how these two are connected with variety of Mango?
Why do you think RAPD and PCR is the same? any reference?
Mango Mapping to Molecular Biology with concepts like PCR(Polymerase Chain Reaction) and RAPD(Randomly Amplified Polymorphic DNA):
A very interesting discussion took place among CUBists on RAPD and how it is connected with PCR. Many of us seem confused about PCR which is taught in 12th but this context of local mango variety gave an opportunity to understand PCR as well as RAPD and its connection with PCR.
This is My version of it:
Ok so to understand RAPD we need to know about PCR 1st.
So PCR is a Polymerase Chain Reaction in which if you have a small portion of DNA you can do its amplification or in simple words, you can increase the amount of DNA so as to study it.
So RAPD is a PCR-based technique but it’s slightly different from PCR.
RAPD is Random Amplification of polymorphic DNA. In RAPD will you amplify the DNA but the portion of DNA you want to amplify isn’t specific (because we don’t know about the particular set of base pairs. So when we will add primers, primers will Randomly attach to the complementary base pair on DNA and amplify it.)
In PCR the portion of DNA you want to amplify is specified but in RAPD the portion of DNA you wanna amplify isn’t specified.
So you get the different lengths of DNA in RAPD (length:- number of nucleotide base pairs)
Is it true that a grafted Mango tree has three different types of Genetic material? One of the graft, second of the scion and the third of the seed of the fresh fruits? @Theertha @Abhi0703 @Sunita123 @PChitralekha and others
Yes i think it’s possible.
Graft is a different plant, scion is different plant and the fruits that will form from scion will have different genetic material due to cross pollination.
Let’s find out!!