Understanding the Genetic Diversity Study, by Using RAPD Techniques Among varieties of Mangoes



Summary of 14/05/2022 Chatshaala☘️

:shamrock:We started discussion with Mango mapping .

:shamrock:Also discussed with the different varieties of mangoes and their local names .

:shamrock:Then we disscussed about the Article send by @⁨Arunan Sir⁩ .That is about the RAPD analysis of different mango varieties of a thaluk in Thiruvananthapuram ,kerala .

:shamrock:Then we come across the term RAPD ,what is RAPD ,is this the same of PCR!!!

:shamrock:Then we started to discussion on PCR ,Polymerase chain reaction .

:shamrock:PCR is a technique used to amplify the DNA.In one cycle we get 2 sequence DNA.

:shamrock:first the double stranded DNA seperated in to two by breaking the H bonds using specific heat 95°c.

:shamrock:then we got two seperate strands,Strand 1 and strand 2 .
:shamrock:Then we add Primers to the 3 ’ end of the strand 1,that is complementary to strand 1,cooling at 54 °c.
:shamrock:with the help of DNA polymerase corresponding complementary strands are formed .

:shamrock: @⁨Sunitha Mam⁩ mentioned about that here that is Taq polymerase .

Questions to be disscussed :
:maple_leaf:why PCR is used ,if we got 2 DNA strand from one cycle what is the purpose of it !?
:maple_leaf:what is the term indicate, Polymerase chain reaction !?
:maple_leaf:what the process occur in RTPCR !?
:maple_leaf:Why Taq polymerase is used here !?
:maple_leaf:When compare to PCR ,what is RAPD!?


So how is PCR connected with RAPD? And how these two are connected with variety of Mango?

Why do you think RAPD and PCR is the same? any reference?