Date: 01st August 2021
[7:50 AM, 8/2/2021] @magpie Nematode moving on rava sugar vinegar medium 10minutes after transfer 1/8/21 Homelab Kolkata Batul
[7:51 AM, 8/2/2021] @magpie No movement of nematodes on the rava sugar vinegar medium 24hrs after transfer, the whole plate has been scanned have they gone deep in the rava or are they dead?
[8:27 AM, 8/2/2021] P. Chitralekha: Yes. Great work Shraddha. How did you provide continuous dark condition?
[9:08 AM, 8/2/2021] @Lydia : As a testable hypothesis, we can state that if adult flies emerge from the trapped pupae kept inside the bottle, then this means that there is no harm in detaching the pupa from its surface onto which it sticks during pupation or that it won’t harm the pupa in the process of development, and hence we can observe its further developmental stages.
[9:17 AM, 8/2/2021] @Lydia : There is a lot of condensation in this bottle @Aswathy Suresh sN nattika Drosophila
What do you do immediately after you pour the medium into the bottle?
Do you allow the media bottle to cool down?
[9:20 AM, 8/2/2021] @Arunan : What are the fifteen stages of pupae, according to the article? @Janhvi Deshmukh
[9:21 AM, 8/2/2021] @Arunan Daily update please! Expectation, Observation along with interpretation, please!
[9:33 AM, 8/2/2021] @Shraddha276 : Thank you😊 @Sunita Chahar ma’am @Batul Pipewala Kolkata @P. Chitralekha
[9:33 AM, 8/2/2021] @Shraddha276 : Yes @Batul Pipewala Kolkata mam leaves opened as well as closed time is most important. I think on basis of that only we can conclude whether 24hrs continuous light & dark exposure affected or not.
[9:34 AM, 8/2/2021] @Arunan : @KIRAN CUBE
[9:34 AM, 8/2/2021] @Shraddha276 :I Kept plants in box like place under cupboard & this covered by another box by placing half inside & half outside.
So it’s helping me to maintain continuous dark conditions & in aeration also.
[9:36 AM, 8/2/2021] @Arunan : Sorry, sent as an appetizer for Kitchen Lab Movement!
Looking for the real ones, from others!
[9:37 AM, 8/2/2021] @Arunan : Any news on Pagalapos studies? @Lydia
@Hina Mudgal @Lakshmi PJ @KIRAN CUBE @P. Chitralekha@Aditya Joshi
[9:38 AM, 8/2/2021] @Arunan : Looking for Monsoon Fast Plants @Shraddha@Yash @Lakshmi PJ @KIRAN CUBE and others.
[9:42 AM, 8/2/2021] @Arunan : @Lydia Please give a design stepwise, so that all of us can test your Hypothesis, in our HomeLabs
[9:44 AM, 8/2/2021] Arunan: Good question @Lydia cUBe Drosphila
Can @Aswathy Suresh @Aastha Ahuja @Janhvi Deshmukh comment?
What is the comment by @Lydia ?
[9:46 AM, 8/2/2021] Batul Pipewala : Are there two set ups 1 and 2?How are they different
[9:46 AM, 8/2/2021] P. Chitralekha:
[9:46 AM, 8/2/2021] Anshu Kadam: @Arunan sir I’m not sure in which soil sample we will find C. elegans. But according to our previous findings and observations, we found Caenorhabditis genus nematodes in soil sample of Jamun tree of Ratnam College, Bhandup and Almond tree in HBCSE surroundings.
@Batul Pipewala which soil sample are you using? And if possible for you, then try getting soil sample from some fruiting trees!!!
[9:47 AM, 8/2/2021] Arunan HBSCE: Any one has Mango trees in their locality in sprouting, flowering or fruiting in the First week of August?
@Lakshmi PJ @Dr. Nisy Prasad @Shrudhiga @KIRAN CUBE @P. Chitralekha Cube JNU @Batul Pipewala @Lydia@Ishita and all others
[9:48 AM, 8/2/2021] Batul Pipewala: Please share pics of your Caenorhabditis nematodes isolated from jamun/badam trees
[9:50 AM, 8/2/2021] DP: Is there fruiting still seen anywhere, esp in the northernmost parts? @MANZOOR JAVAID in J&K or may be even in Delhi, Haryana Punjab areas?
[9:52 AM, 8/2/2021] Arunan: *commonly known as passion fruit, is a vine species of passion flower native to southern Brazil through Paraguay and northern Argentina.[1] It is cultivated commercially in tropical and subtropical areas for its sweet, seedy fruit.*Passiflora edulis - Wikipedia
[9:54 AM, 8/2/2021] Shraddha : No mam, there is only one set up.in 1st picture I was trying to show how plants kept inside & in 2nd picture position of box used to covered the plants.
[9:55 AM, 8/2/2021] Arunan : Comments please @Seethalakshmy CUBE @Shrudhiga and all Collaborators of Ant Model system!
You have made me fall for these!
Please join and let’s take it forward @Seethalakshmy CUBE
[9:58 AM, 8/2/2021] Arunan : So spiders are not insects? @Seema
Any reference?
[10:00 AM, 8/2/2021] Arunan: Amazing! @Seethalakshmy CUBE
Any reference you could find of these insects?
@Seethalakshmy CUBE
[10:02 AM, 8/2/2021] Arunan: Wonderful! @Sunita Chahar Same result means what? Please clarify.
[10:03 AM, 8/2/2021] Arunan : What do you mean by both sets? @Sunita Chahar
[10:03 AM, 8/2/2021] CUBist: Really amazing for doing this sir… What are this species called as sir? Do they have a local name?
[10:14 AM, 8/2/2021] CUBist: Plants kept in continous dark also did not close the leaves after 4 to 5 days
[10:25 AM, 8/2/2021] CUBist: According to the article we see fifteen stages of pupae sir…
1)stage 1:white puparium
2)stage 2: brown puparium
3)stage 3: bubble prepupa
4)stage 4:Bouyant
After the 4 rth stage we see in the article phenerocephalic pupa as a heading…we can stuck here what is phenerocephalic pupa?so we can read from 5 th stage to 13 stages so what we guess from here the pupa start to develop a bodypart …
5)stage 5:a)Malpighian tubules migrating
B)white Malpighian tubules
6)stage 6:green Malpighian tubules
7)stage 7: yellow body
8)stage 8: yellow eyed
9)stage 9:Amber
10)stage 10:red eye bald
11) stage 11: a)head bristle b) thoracic bristles
12)stage 12: a)wings tips grey b)wings grey
13) stage 13:wings black
14)stage 14 :mature bristles
15)stage 15:a) meconium b)eclosion
[10:28 AM, 8/2/2021] Arunan: What do we mean in Continuous Light?
What was the pattern of folding and unfolding of leaves, before keeping your Phyllanthus plant in Continuous light? @Sunita Chahar
Which species of Phyllanthus are you keeping in Normal light and in Continuous light?
Will be interesting get more clarity so that more cubists can join from thier HomeLabs!
[10:31 AM, 8/2/2021] Arunan: What is amazing about it? @Shrudhiga
I just kept a ruler with millimeter scale, as a reference to get the measurement of the ants!
Don’t you think that can tell us many things?
[10:31 AM, 8/2/2021] Arunan: What is amazing about it? @Shrudhiga
I just kept a ruler with millimeter scale, as a reference to get the measurement of the ants!
Don’t you think that can tell us many things? @Shrudhiga
[10:36 AM, 8/2/2021] CUBist: Yes sir… Size is also a criteria for the species identification of ants… There are also other criterias like color, body organization, and other special features of any one individual species
[10:38 AM, 8/2/2021] Arunan : *In my childhood, such ants were generally called “small black ants”! Sometimes, they were called ‘chonan’ in South Kerala. @Shrudhiga @Seethalakshmy CUBE
There used to be black chonans and yellow chonans!
However, in Mumbai, no one has names to call any ants to differentiate them! All are Ants!
They are all potential victims of insecticide spray, if they surpass their limit!
[10:39 AM, 8/2/2021] Arunan: Not clear @Sunita Chahar
[10:42 AM, 8/2/2021] Arunan: Please tell where you are stuck.
Other cubists will help! @Sidhy Kerala @Lakshmi PJ @Aswathy Suresh @Yash and others.
It will be a restart for them too in Cell phone microscopy @Aswathy Suresh @Janhvi Deshmukh @Aditya Joshi
[10:46 AM, 8/2/2021] Arunan HBSCE: The Phyllanthus in Continuous blight slept only for 3 to 4 hours? @P. Chitralekha
What about the Control plants, kept in normal sunlight light? @P. Chitralekha
@Lakshmi PJ what is your comment based on your experience?
[10:50 AM, 8/2/2021] Arunan : Won’t according to seasons, the length of day time change? More in summer months than in winter months, with Equinox in March & September having almost equal day and night length? @Sunita Chahar
[10:55 AM, 8/2/2021] Arunan : Ehy not we organize more collaborators to start looking at what happens to control plants, now on through Equinox times, 2nd & 3rd week of September, from across the country?
A COLLABORATIVE PROGRAM to celebrate 500 Days of CUBE HomeLab Movement! @P. Chitralekha @Sunita Chahar @Lydia @Batul Pipewala
[10:55 AM, 8/2/2021] Arunan : Why*
[11:02 AM, 8/2/2021] Arunan : Do you agree? @Janhvi Deshmukh and why?
[11:05 AM, 8/2/2021] CUBist: Yes sir I agree with chitra mam because there are three plant one plant are from different species one looks like phylluntus but leaf are so small and Also have thorns and we can’t se thorns in phyllunthus …and one is phyllunthus amarus sir …@Arunan
[11:06 AM, 8/2/2021] Arunan : Second time, this line is giving you trouble! @Aswathy Suresh
What do you think has happened?
Was there sometime you had more second generation flies in this bottle? @Aswathy Suresh
Don’t you have some other SLBK A bottles like SLBKA18, SLBKA19, with larvae, unhatched Pupae and adult flies? @Aswathy Suresh
[11:10 AM, 8/2/2021] KIRAN CUBE: Ohhh God😳 Why there’s some much of water condensation in this bottle?.
[11:11 AM, 8/2/2021] Arunan: What is your summary headline of all SLBK line fly stocks?
Please announce in one big headline! @Aswathy Suresh
[11:14 AM, 8/2/2021] Arunan : Why no news about SLBK C bottles? @Aswathy Suresh
Or, did I miss it because of too elaborate reporting style?
[11:18 AM, 8/2/2021] CUBist: Mushroom growth under our bathroom sink
[12:04 PM, 8/2/2021] Arunan E: Wow! @Ujaan Banerjee
Can you post another photo with better lighting?
Please give Location, Date and Name of Data Collector along with all photos.
Please post more!
[12:09 PM, 8/2/2021] Batul Pipewala: @Arunan@Anshu Kadam @Aswathy Suresh the nematodes are not liking the rava sugar vinegar medium
[12:10 PM, 8/2/2021] Arunan: How do we show the possibility of confusion between simple leaves of Phyllanthus and leaflets of some compound leaves, like Gulmohar tree?
@Janhvi Deshmukh @Sunita Chahar @P. Chitralekha @Yamal Gupta Cube KMC Delhi Botany Prof. @Shyam T.G
[12:15 PM, 8/2/2021] Arunan : Interesting! Why no water of condensation in SLBKB20 bottle in contrast to SLBK A21 bottle transferred the same day from the common stock medium?
@Aswathy Suresh Drosophila any urgent guess?@KIRAN @Yash @Lydia
[12:18 PM, 8/2/2021] Arunan: Here again, no water of condensation compared to SLBK A21 bottle of the same day! Let’s have some analysis! @Aswathy Suresh @Yash @Lydia
[12:21 PM, 8/2/2021] Arunan : Wow! @Aswathy Suresh
From just 2 flies transferred almost a month ago, so many pupal cases & so many flies! @Aswathy Suresh
How do you explain this? @Aswathy Suresh
[12:24 PM, 8/2/2021] Arunan : How about sprouting leaves? We can see some leaves on top with lighter colors! @Dr. Nisy Prasad Cube What do you think that is?
[12:26 PM, 8/2/2021] Arunan : What is meant as dormant state? What is its significance?
[12:28 PM, 8/2/2021] Arunan : What is your objective? And what is your conclusion? @Shraddha
[12:30 PM, 8/2/2021] Arunan : GRAT! @Janhvi Deshmukh Is this t just a chance happening or the bee is having some specific interaction like looking to flowers and feeding nectar? @Janhvi Deshmukh
[12:52 PM, 8/2/2021] Batul Pipewala: @Aswathy Suresh please collaborate as you too have microworms growing in rava medium
[1:37 PM, 8/2/2021] Anshu Kadam : Why?? @Batul Pipewala. Are you not able to see any nematodes?
How many hours since you transferred the nematodes to the medium?
Please share videos and photos of Rava Sugar Vinegar medium!!
[2:07 PM, 8/2/2021] CUBist: Open Bait setup janhvi deshmukh homelab bajaj collage of science wardha…
[2:07 PM, 8/2/2021] CUBist: It’s a second interlarva
[2:11 PM, 8/2/2021] Arunan: Please give essential details of the Dates when bait was started & Date of observation, Location & objective along with each photo @Janhvi Deshmukh
[2:11 PM, 8/2/2021] Arunan: Please give some reference link. @Janhvi Deshmukh
[2:19 PM, 8/2/2021] CUBist I was got 13,14 flies in my open bait setup on 29/07/2021 in my bait I used tomato and cucumber as a bait…at 8.30pm I got flies on it and closed it with the box tip …next day I observed 2 gravid fruitfly in the bait I kept this bait in my backyard near the dishwashing area…due moisture some flies are died …on 01/08/2021 I seen some larve on my bait …and in today observation I seen a second insterlarve and also some 1 insterlarve…
[2:20 PM, 8/2/2021] CUBist: This is 28/07/2021 when I trapped 13,14 flies …at 8.30 pm
[2:21 PM, 8/2/2021] CUBist: This video on 01/08/2021 when I see some larve moiving in bait…
[2:22 PM, 8/2/2021] CUBist: @Arunan @Aswathy Suresh @KIRAN CUBE @Batul Pipewala
[2:24 PM, 8/2/2021] Batul Pipewala: In how many days do you expect to see the pupae?
[2:46 PM, 8/2/2021] +91 03221: Two day mam @Batul Pipewala
[2:50 PM, 8/2/2021] Arunan: A slice of the 1st day of CUBE National Meet 19, 20, 21March 2021 5-30pm to 9-30 pm https://webinar.hbcse.tifr.res.in/b/kir-vgc-6jf
[2:53 PM, 8/2/2021] Arunan : Why? @Janhvi Deshmukh What is the reasoning?
[3:44 PM, 8/2/2021] Yash :
[3:45 PM, 8/2/2021] Yash: @Batul Pipewala there is another nematode that is moving here… Near the soil… @Anshu Kadam @Arunan@Shivam CUBE
[3:46 PM, 8/2/2021] Yash: Wow @Janhvi Deshmukh
Can you share the reference to this Article…
Which pupae are you able to See in the Bait that you have?
@Aswathy Suresh @Arunan@Lydia
[3:48 PM, 8/2/2021] Yash : Lets hope for the gravid looking like fruit fly in the fresh TRS-V media
To lay eggs @Aswathy Suresh sN nattika Drosophila
[3:49 PM, 8/2/2021] Yash : @Aswathy Suresh
[3:50 PM, 8/2/2021] KIRAN CUBE: 500th Day of CUBE chatShaala: 01st August 2021
43 CUBists had joined the webinar
INDIA
Assam
Jorhat: Ishita Sonowal
Delhi
New Delhi: Raj Yashwantrao Patil
Maharashtra
Bandra: Saida Sayyed
Anchal Yadav
Manasi Parab
Kinjal Gohli
Vinay Gahlot
Gautam Maurya
Karan Fulwariya
Divya Rao
Jasmin Shaikh
Sachi Bind
MC Arunan
Chaitanya Gaikwad
Raj Ptail
Sunita Chahar
Sarita Kumar
Anjani Kumar Kashyap
Sachin Pradhan
Anshu Kadam
Rachana Acharya
Shalini Gautam
Lydia Mathew
Shraddha sonavane
Aditya Joshi
Khan shahreen
Janhvi Deshmukh
Priti Kanade
Kareena Dhuri
Jharkhand
Man Masih Beck
Kerala
Aswathy Suresh
Vishnupriya
Seethalakshmi
Lakshmy
Arunima
Shyam TG
Kolenchery
Abhijith Vinod
Ajin
Uttar Pradesh
Moradabad: Kiran Yadav
Uttrakhand
Darshana V. …
[3:51 PM, 8/2/2021] Yash: Breaking News Update
More fruit flies observed in Fruit fly cultures at 9th day from Transferring a Single Gravid fruit fly!
Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from Locality
Location - Prabhadevi, Mumbai
Gravid fruit fly Transferred on - 24/07/2021
3 day observation
SLPbA1
Breaking News - ~ 15 First generation Fruit flies arrive at the 7th Day of Transfer ring Gravid Fruit fly
Date and time of video - 31/07/2021 - 11:45 AM
31/07/2021
Observation
No of Fruit flies - ~ 15
No of pupae - ~ 70
No of larvae - - 12 - 3mm larvae, 5 - 2mm
1/08/2021
Observation
No of fruit flies - ~40
No of unhatched pupae - 40
2/08/2021
Observation
No of fruit flies - ~50
No do unh…
[3:53 PM, 8/2/2021] CUBist: Actually I didn’t see any pupae i see only the larve @Yash Isc Xmas Cube
[3:54 PM, 8/2/2021] CUBist: @Yash Isc Xmas Cube
[3:59 PM, 8/2/2021] Yash I: Location - Prabhadevi, Mumbai
Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from Locality
Gravid fruit fly Transferred on - 24/07/2021
SLPbB1
31/07/2021
Observation
No of Fruit flies - 8
No of pupae - 56
No of larvae - 3- 3mm Larvae, 12 - 2mm larvae
1/08/2021
No of fruit flies - ~ 25
No of unhatched pupae - 35
No of larvae - 8 - 3mm larvae
2/08/2021
No of fruit flies - ~ 30
No of unhatched pupae - ~30
No of larvae - 12 - 3mm larana
alert - No of dead flies - 8 fruit flies
[4:07 PM, 8/2/2021] Yash: Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from Locality
Update of Single species culture of Fruit flies
Location - Prabhadevi, Mumbai
SLPbC1
31/07/2021
Observation
No of Fruit flies - 5
No of pupae - ~ 60
No of larvae - 10- 3mm larvae
1/08/2021
Observation
No of fruit flies - 20
No of unhatched pupae - 32
No of larvae - 7 - 3mm larvae
2/08/2021
Observation
No of fruit flies - ~35
No of unhatched pupae - ~ 40
No of larvae - 8 - 3mm larvae
[4:10 PM, 8/2/2021] Dr. Sarita Kumar : Hi Kiran…my name has been entered under Maharashtra😊
[4:11 PM, 8/2/2021] KIRAN CUBE: Thanks for the correction.
We will do the needful
[4:13 PM, 8/2/2021] Yash: Guess? guess!!.. Different Stages of Pupae seen in SLPbC Culture! -
Date - 2/08/2021.
Location - Prabhadevi, Mumbai
I am guessing the one marked in Orange marked is an empty pupal case.
Blue marked - Yellow Pupae
green marked - Wing stage Pupae?!
@Janhvi Deshmukh @Arunan HBSCE sir @KIRAN CUBE maam @Batul Pipewala Kolkata maam @Aswathy Suresh sN nattika Drosophila @Lydia cUBe Drosphila @Isha Pawle @Aastha Ahuja
[4:16 PM, 8/2/2021] KIRAN CUBE: Woow am i able to see the red color of eye in the right bottom pupa marked under green circle.
[4:34 PM, 8/2/2021] Dr. Sarita:
[4:58 PM, 8/2/2021] Seethalakshmy CUBE: Great @Yash
What is the black dot present it?
Empty pupal case as well as yellow pupal case can able see this black dot.
Green marked one it’s not clearly visible, so i don’t know is present or not.
[5:00 PM, 8/2/2021] Aastha : I think that black dot is the mouth part of the larvae in blue and orange circle
[5:00 PM, 8/2/2021] Seethalakshmy CUBE: What is wing stage pupae?
Can you explain?
[5:01 PM, 8/2/2021] Aastha : Why does the color of early pupa in blue circle to the late pupa in orange circle change to dark brown from whitish ?
[5:02 PM, 8/2/2021] Aastha: Green circle pupa has wings in it they are the black color lines present on both sides of pupa
[5:05 PM, 8/2/2021] Aastha: Arrow indicates the black line in the pupa which is wing @Seethalakshmy CUBE , photo credits @Yash
[5:21 PM, 8/2/2021]CUBist: I think the yellow round is stage 7 : yellow body
[5:25 PM, 8/2/2021] Seethalakshmy CUBE: where did you find these ant? @Arunan HBSCE sir
Size of the ant is 2 mm right?
Is it lonely? Have you observed what it does?
[5:29 PM, 8/2/2021] CUBist: And green circle is a stage 12 :wings tips grey I guess
@Yash
[5:47 PM, 8/2/2021] Aastha : Can you please resend the reference for stages of pupa I think it was shared earlier but my bad I missed it
[5:57 PM, 8/2/2021] Seethalakshmy CUBE: I think this is a worker ant.
Beause according to it’s size as well as it’s behavior
Only the worker ant searching food