Major goof-ups I made:
1)Added too much salt, which caused the precipitation of the protein!
2)The perfume I used did not indicate the amount of alcohol it contain, possibly a lower amount of alcohol wouldn’t have precipitated the DNA.
3)Using papaya juice and onion juice did not align with my objective, which was to isolate onion DNA.
Biochemistry of DNA Extraction!
1)Breaking the cells (cell disruption) and release of the DNA can be accomplished using appropriate methods including grinding the living tissues.
(2)Removing membrane lipids by adding a detergent.
(3)Cellular proteins including histone that bound to the DNA can be removed by extracting with a phenol-chloroform mixture or precipitating with sodium or ammonium acetate or adding a protease.
(4)Precipitating the DNA with alcohol (usually isopropanol or ethanol). Since DNA is insoluble in these alcohols, it will aggregate together, giving a pellet upon centrifugation. @Amirtha CUBE @Enas CUBE @Rechel CUBE and others.
https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/dna-extraction
Biochemistry of DNA Extraction!
1)Breaking the cells (cell disruption) and release of the DNA can be accomplished using appropriate methods including grinding the living tissues.
(2)Removing membrane lipids by adding a detergent.
(3)Cellular proteins including histone that bound to the DNA can be removed by extracting with a phenol-chloroform mixture or precipitating with sodium or ammonium acetate or adding a protease.
(4)Precipitating the DNA with alcohol (usually isopropanol or ethanol). Since DNA is insoluble in these alcohols, it will aggregate together, giving a pellet upon centrifugation. @Amirtha CUBE @Enas CUBE @Rechel CUBE and others.
https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/dna-extraction
[06/11, 4:57 pm] Arunan sir : Do you agree with this explanation from the reference sent by @Theertha
@~Mohammad Yousuf Ganaie @Kiran CUBE @Rechel CUBE @Enas CUBE and others.
“According to Rice (2018), the method involves three necessary steps, namely, lysed, precipitation, and purification. The first step is lysed to break down the cells so the DNA in the nucleus will be released after the cell membrane is disrupted. The second step is precipitation. To get pure DNA, the DNA will be separated from proteins and other cells component by degrading the DNA and protein with ethanol or isopropanol.”
https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/dna-extraction#:~:text=According%20to%20Rice,or%20isopropanol.