@bivasnag @Sjuday2527 @drishtantmkawale On behalf of @Imam I’ll answer some of the questions asked above…
The components used in corn meal agar medium are:
- Dextrose
- Sucrose
- Maize powder
- Yeast extract powder
- Agar agar powder
- Distilled water
Acids added
- Orthophosphoric acid
- Propionic acid
What could be the reason for using such a media??
When we explain this to a common man…how do we tell them that we use such components!!! Everyone wont understand what these components are!!! Also they would think “for culturing such flies…why so many components”
So in simple terms…
Like how human beings require carbohydrates, sugars, proteins, vitamins & minerals in their daily diet…in the same way these flies need them too if we want to culture them!!
So Maize flour is a source of carbohydrates, etc… dextrose is a simple sugar and a source of glucose (energy) for the larvae as its a simple sugar, sucrose a disaccharide for the flies, yeast extract a source of proteins and other nutrients present in it…
Agar is a solidifying agent as we cant culture or keep the flies in a liquid medium…
Why distilled water…for avoiding other ions or any minerals or anything in the water that could cause harm for the flies…seeing to it that we maintain a neutral pH as well…
Acids…orthophosphoric acid has antibacterial property while propionic acid has antifungal property!!!
Media bottles for culturing flies…