Soon after the day of making these 5 beaker cultures, I observed my Hydras so as to see whether there is any change or growth or increase in number of Hydras or not,etc. I was amazed seeing that just after one day of making culture I got to see budding occurring in my hydras. Which certainly means they were growing happily the 5 beakers are labeled as HC1, HC2âŠHC5. As mentioned earlier there were 3hydras in each.
Iâll check tomorrow and see whether the buds have detached from the parent or not, and will also see it budding has occurred in those Hydras in which no bud was seen.
Pitch in guys and ask more questions (when does it float, how often does it eat, is hydra having a digestive system,etc) to get many doubts clarified. @drishtantmkawale@jtd@pratittodkar@Akshitha
Rod and cone cells in the human retina contain proteins called opsins that change shape when light strikes them. This causes another type of protein, an ion channel, to generate an electrical signal along nerves connecting the eye to the brain â a process called phototransduction.Hydras have the same types of opsins and ion channels as we do.
Well , Iâm not sure at present how would I do but yes definitely Iâll see upon this and will work out on it. Any ideas for the design @drishtantmkawale@pratittodkar and all
Do they fire the poison every time there is a change from light to dark or just every time there is darkness? Is the poison acidic or basic and whatâs itâs pH? What is the minimum water level that hydraâs need to survive? How much poison will they secrete in a single instance?
They donât have any nerves. The hydra has a nervous system characterized by a nerve net. A nerve net is a collection of separate, but âconnectedâ neurons. Neurons are connected by synapse(https://faculty.washington.edu/chudler/synapse.html). Communication between neurons can be in both directions at the synapse within a nerve net. The nerve net is concentrated around the mouth. Unlike higher animals, the hydra does not have any grouping of nerve cell bodies. In other words, there are no ganglia.
As I mentioned my main motto is to increase their number but want to do some interesting stuff on it âŠcan you design an interesting experiment using Hydras with a big objective.
Although attached to the substratum by the basal disc and usually standing erect, the Hydra has several methods of locomoÂtion. Usually it bends down and attaches the tentacles to the subÂstratum with the help of the glutinant nematocysts.
It now releases and removes the basal disc to a new position and again stands up by disengaging its tentacles. This type of locomotion is known as walking. It may bend its body and perform looping movement like that of a leech (Fig. 62 A). When in a hurry, the animal runs by performing a series of somersaults (Fig. 62 B).
Sometimes it walks inverted by using the tentacles as legs. Again it may glide considerably by dragging the basal discâthe cells of which have special power of throwing out pseudopodia like an Amoeba.
Occasionally Hydra secretes a bubble of gas under its basal disc and floats about by the hydrostatic action of the gas bubble. A Hydra may climb by attaching its long tentacles to a distant object. Then releasing the basal disc and contracting the tentacles the body is drawn up, and it resumes its normal position on the new substratum.
It is to be noted that:
(1) Fixation is effected by the mucus- secreting gland cells in the epidermis of the basal disc and the glutinant nematocysts with sticky threads on the tentacles.
(2) Shortening the length of the body is due to the contraction of the muscular processes of the ectodermal epitheliomuscular cells.
(3) Increasing the length of the body is produced by contracting the muscular processes of the endodermal nutritive-muscular cells.
What is your expectations today? @Sjuday2527?
Are the number of hydra increased?
Are the hydra having buds?
How many beakers/tanks of hydra are present?
What is the total count of hydra?
What is your feeding regime? How many moina in beakers, how many in tanks?
Actually I searched on this a long time back but couldnât find any information, well now I have got one stating,
Hydra have been classical model organisms for over 250 years, yet little is known about the toxins they produce, and how they utilize these toxins to catch prey, protect themselves from predators and fulfill other biological roles necessary for survival. Unlike typical venomous organisms the hydra allomonal system is complex and âholisticâ, produced by various stinging cells (in the hunting tentacles and body ectoderm) as well as by non-nematocystic tissue. Toxic proteins also fulfill novel, non-allomonal roles in hydra. This review described the toxins produced by hydra within the context of their biology and natural history. Hydra nematocyst venom contains a high-molecular weight (>100 kDa) hemolytic and paralytic protein and a protein of approximately 30 kDa which induces a long-lasting flaccid paralysis. No low-molecular weight toxicity is observed, suggesting the lack of âclassicalâ 4-7 kDa neurotoxins. The occurrence of a potent phospholipase activity in the venom is supported by the detection of several venom-like phospholipase A2 genes expressed by hydra. Hydra also produce toxins which are not part of the nematocyst venom. In the green hydra, Hydralysins, a novel family of Pore-Forming Proteins, are secreted into the gastrovascular cavity during feeding, probably helping in disintegration of the prey. Other putative non-nematocystic âtoxinsâ may be involved in immunity, development or regulation of behavior. As the first venomous organism for which modern molecular tools are available, hydra provide a useful model to answer many outstanding questions on the way venomous organisms utilize their toxins to survive.
Well, I have kept them in a 250ml beaker with 100ml of DC water filled. The less will be the water the easier it would be to catch itâs pretty moina. It shouldnât be even too less, at present whatâs this too less, I donât know but yes in 100 ml thereâs no problem to the Hydras thatâs what I can see as they are increasing in number day by day.
My feeding pattern was around 4 moinas to each hydra. So in the starting i.e. on 19-9-19 I had put approximately 12-15 moinas in each beaker which means every Hydra would have got 4-5 moinas.
Updating about the tank culture which I had missed to mention, I had prepared this tank culture of Hydra just a day before these beakers were made i.e on 18-9-19 .
I had put 5hydras in it and had given them approximately 40-50 moinas just with a logic that they would eat more and grow faster in a tank .
But the next day i.e on 19-9-19 I observed that there were only 2 Hydras I was thinking where did the rest of the 3hydras go ???
But when I saw the count of moinas there were just 1 or 2 left. I feeded them again and after 2 days I saw 4 I was happy at least they are increasing now. After a few days on 26-9-19 I put some algae from a previous tank so that this algae doesnât allow the debris to be accumulating. Even if you see in moina cultures with algae, you wonât be finding debris. But why ? What does algae feed on ???
This is the picture after inoculation of algae in my tank.
As you all can see here there is another beaker HGEC (HYDRA GROUP ELPHINSTONE COLLEGE)
which they had made in the last workshop , Iâm feeding that beaker too and Iâm seeing increase in number in that beaker tooâŠI have 2more beakers which @jaikishan used to maintain Iâll send their pic as well by tomorrow. Those 2beakers have 35 and 28hydras respectively.