Moina culture - CUBE Kolenchery

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Protocol followed to grow Moina macrocopa using yeast:

  1. Take 50ml of dechlorinated water in a beaker.
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    [center]50ml dc water[/center]
  2. Put 4-5 yeast granules into this 50ml of water. Yeast powder maybe used as well, but granules are easier to get an idea of the exact amount of yeast dissolved.
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    [center]Yeast granules[/center]
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    [center]Addition of 5 yeast granules into 50ml dc water[/center]
  3. Stir this really well until the yeast granules are completely dissolved in the water. You may heat the water to speed up this process, but ensure that the yeast solution has completely cooled down before adding into the Moina culture.

Unlike culturing of Moina macrocopa using milk, it isn’t necessary to add this yeast solution everyday. We observed that the Moina have stayed alive for 2 complete days without addition of yeast solution. We’ll proceed to check how long the Moina will survive without addition of yeast solution.

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