582nd Day of CUBE ChatShaala: How to develop a medium for fruit flies?

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CUBE

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Timing:5.30 pm to 9.30 pm

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CHATSHAALA for eg. (KiranYadav-HBCSE, Mumbai)

Summary of discussion

What all nutrients are required by an organism? What are the essential nutrients?

Nutrients Nutrients
Carbohydrates Proteins
Fats Minerals
Vitamins water

How do we get Energy from carbohydrates ( Rice, Chapati etc )?

What are the important groups of proteins in the organisms? What are major roles of proteins in organisms?
DNA polymerase is an enzyme which help in synthesis but do we have DNA polymerase in our diet or is it metabolized in the body?

All these were the attraction of yesterday’s discussion and this was discussed in the context that HOW TO DEVELOP A MEDIUM FOR FRUIT FLIES? what all ingredients we should include in order to make a good medium to culture fruit flies in home labs?

IMPORTANCE OF SINGLE LINES

  • What’s single line Cultures? How do we make single lines?
  • Transferring one gravid/pregnant fruit fly in a single bottle?.
  • How to isolate one single gravid fly from the mixed culture bottle which has approx 50 fruit flies?
  • La belling of bottles
  • How do we label the bottles?