10th August 2021, 9.30pm to 11th August 2021, 9.30pm
9.30 pm Janhvi Deshmukh
[8/10, 9:40 PM] Janhvi Deshmukh: This is my open bait setup in which l see two types of pupae and today I see some water condensation in bait I afraid that when pupae hatched out the flies are died due to water condensation so what can I do that time?
Location:wardha
Date :10/08/2021
Data collector : janhvi deshmukh
Janhvi deshmukh homelab bajaj collage of science wardha Maharashtra
[8/10, 9:41 PM] Man Masih Beck RANCHI CUBE: So you’re having Pupae of houseflies and pupae of fruitflies in the same container. Are they interacting with each other?
[8/10, 9:44 PM] Man Masih Beck RANCHI CUBE: Is your lid detachable?
[8/10, 9:44 PM] Janhvi Deshmukh: No man they not interacting each other
[8/10, 9:44 PM] Janhvi Deshmukh: Yes
[8/10, 9:46 PM] Man Masih Beck RANCHI CUBE: why dont you transfer your lid into some other larger transparent container? like plastic bags or larger plastic box? This way, you can atleast save the pupae attached to the lid.
[8/10, 9:51 PM] Janhvi Deshmukh: Ya I do that but what about other pupae which in side of box? @Man Masih Beck RANCHI CUBE
[8/10, 10:14 PM] Aswathy Suresh CUBE: So, How many culture bottles currently do you have In Prabadhavi Mumbai?
[8/10, 10:17 PM] Aswathy Suresh CUBE: Why 17 days old bottle media became liquidy? Is it effected your flies?
[8/10, 10:24 PM] Aswathy Suresh CUBE: FRUIT FILES COLLABORATORS
Different parts of country.
Aswathy Suresh (me) - Bottukadavu,Thrissur Kerala.
Yash Shergare - Prabadhavi, Mumbai.
Isha Pawle - Worli, Mumbai.
Rechal Tirkey - Morabadi, Ranchi.
Kiran Yadav - Moradabad, Uttar Pradesh.
Janhvi Deshmukh - Wardha, Mumbai
Aastha Ahuja - Faridabad Delhi
Ishita - Jorhat, Assam.
Currect Fruit Flies Collaborators (Who are culturing fruit flies in homelab).
[8/10, 10:30 PM] Aswathy Suresh CUBE: One our Hypothesis is Native (Wild growing) D.melanogaster have better olfaction (Smelling capacity) compared to CsBz. Because CsBz - D.melanogaster started to culture almost 70 years ago (1950). Almost 3360 generation passed. There could a chance that mutant will form (Because of several other reasons).
[8/10, 10:37 PM] Aswathy Suresh CUBE: Great @Yash Sheregare CUBE 
.
[8/10, 10:39 PM] Aswathy Suresh CUBE: How many days old culture bottle is this @Yash Sheregare CUBE!? What is your further plan?
[8/10, 10:51 PM] Aswathy Suresh CUBE: Nice Summary . What is your objective? Could you please elaborate?
[8/11, 9:07 AM] Arunan HBSCE: Good! @Aswathy Suresh CUBE
But, @Man Masih Beck RANCHI CUBE & @Aastha Ahuja Cube proposed another Hypothesis that Recently trapped local Fruitflies have better capacity for smelling than Lab cultures of local Fruitflies kept in HomeLab for more than one year.
Please comment. @Aswathy Suresh CUBE @Man Masih Beck RANCHI CUBE @Aastha Ahuja Cube @Seethalakshmy CUBE @Yash Sheregare CUBE
[8/11, 9:23 AM] Batul Pipewala Kolkata: I too agree with this hypothesis @Aswathy Suresh CUBE can try this experiment and prove or falsify this hypothesis.So what all do you need for such an experiment? What plan would you follow please write details @Aswathy Suresh CUBE
VIDEO OF NEMATODE BY BATUL PIPEWALA at 9.28AM
Original Nematode culture set up with soil on 29/7/21 showing dense growth of nematodes 13days later today 11/8/21 Cube Homelab Kolkata BatulP
video shared by Batul Pipewala at 9.43am
Nematode culture B started on 6/8/21 by transferring nematodes from original culture A shows nematode growth six days later pic 11/8/21 Homelab Kolkata BatulP
[8/11, 9:44 AM] Batul Pipewala Kolkata: The culture had 4drops of milk added to it on first day,there is no soil and everyday two drops of water is added twice.
[8/11, 9:53 AM] Arunan HBSCE: Which medium is this? @Batul Pipewala Kolkata
[8/11, 9:55 AM] Man Masih Beck RANCHI CUBE: After calculating we found that around 48 generations have passed in 1 year old culture bottles of @Aswathy Suresh CUBE .
Let’s take a Hypothetical situation:
In human terms, 48 generations ago, humans would have been in pre-Christ era 400 BC(taking human age as 50 years on an average). The way people used to think and behave at that time was quite different than the people now.
Suppose some people were trapped and were being maintained in a closed environment since 400 BC.
Then in 2021, their descendants are being compared behaviourly with that of free human descendants. Don’t you think that there would be a difference in the behaviour of isolated humans vs free humans?
@Aswathy Suresh CUBE @Batul Pipewala Kolkata @Arunan HBSCE @Yash Sheregare CUBE
There is a reason why I believe there would be a difference between behaviours…
[8/11, 9:56 AM] Batul Pipewala Kolkata: Raw potato medium
[8/11, 9:58 AM] Batul Pipewala Kolkata: 
[8/11, 9:58 AM] Arunan HBSCE: Please describe the " kind of interactions which are normal in nature"?@Batul Pipewala Kolkata
[8/11, 10:00 AM] Arunan HBSCE: Any comments? @Aswathy Suresh CUBE @Lydia cUBe Drosphila
[8/11, 10:02 AM] Arunan HBSCE: Yes, the same medium & culture doing well in SLBKE & SLBK B?! @Yash Sheregare CUBE @Aswathy Suresh CUBE
So, what could have gone wrong with SLBK A & SLBK C fly cultures?
[8/11, 10:06 AM] Arunan HBSCE: Please give all essential information like which ants, how old is the set up, what is placed in the two containers and why?
This is especially important since your updates have not been on a daily basis. So it is important to communicate all essential information with each photo. @Seethalakshmy CUBE
[8/11, 10:08 AM] Arunan HBSCE: Do you have a Control photo of the daytime activity? Please always post such Control evidence for people to get convinced. @Seethalakshmy CUBE
[8/11, 10:10 AM] Arunan HBSCE: Please give the essential information like magnification used and what is your guess, etc. @Batul Pipewala Kolkata
[8/11, 10:10 AM] Batul Pipewala Kolkata: Interactions with their natural microbiome consisting of beneficial bacteria,fungi,diatoms,algaearound them,as also mites,snails living in the soil with whom they interact.Their survival and evolution depends on these natural environment which is absent in a lab
[8/11, 10:11 AM] Arunan HBSCE: How about a video of this?
[8/11, 10:13 AM] Batul Pipewala Kolkata: Magnification is 450 times,observation under 45X of compound microscope. Size of the organisms will be in microns.My guess is they.are diatoms or blue green algae
[8/11, 10:14 AM] Batul Pipewala Kolkata: Ok sir
[8/11, 10:16 AM] Arunan HBSCE: Please give essential description with each photo and especially when updates are infrequent when we want others including potential collaborators, to respond.@Saida Sayyed
Please repost with essential details @Saida Sayyed
[8/11, 10:20 AM] Arunan HBSCE: Great! @Aditiya Joshi CUBE What is your guess on the species that you see more frequently encounter in Kalyan- Murbad Road? @Aditiya Joshi CUBE
Which other plants are growing fast during monsoon and complete the Lifecycle, which we can consider as Monsoon Fast Plants of Kalyan Locality? @Aditiya Joshi CUBE
[8/11, 10:22 AM] Arunan HBSCE: Which species of Phyllanthus are observed in Kalyan Locality in August?
And which percentage of which species?
[8/11, 10:22 AM] Arunan HBSCE: @Aditiya Joshi CUBE
[8/11, 10:22 AM] Janhvi Deshmukh: Great 
! @Aditiya Joshi CUBE so you can which phylluntus species is that?
[8/11, 10:24 AM] Janhvi Deshmukh: We can’t see fruit here ? @Aditiya Joshi CUBE
[8/11, 10:25 AM] Arunan HBSCE: What is your guess of the species?
Can we develop a simple method to identify the Phyllanthus species growing as weeds near city roads? @Aditiya Joshi CUBE
Please develop one and post for others to refine it. @Aditiya Joshi CUBE
[8/11, 10:26 AM] Arunan HBSCE: Any guess which species? @Aditiya Joshi CUBE
[8/11, 10:26 AM] Arunan HBSCE: Which are the other common plants growing along with Phyllanthus species? @Aditiya Joshi CUBE
[8/11, 10:29 AM] Arunan HBSCE: Please give the details of the survey. What percentage of sites surveyed showed Mosquito breeding sites?
What was the design of the survey?
How was it done?
Who were the participants? @Hina Mudgal Kanpur CUBE
[8/11, 10:30 AM] Arunan HBSCE: How frequent is the rain? Which exact locality in Kanpur? @Hina Mudgal Kanpur CUBE
[8/11, 10:33 AM] Arunan HBSCE: What is the percentage of germination?
Please give some more details like when were seeds sown?
What is your objective?
That will help in others joining and taking cues from your success story @Lakshmy PJ CUBE
What do you say? @Seethalakshmy CUBE
[8/11, 10:34 AM] Arunan HBSCE: Percentage of seeds germinated, out of the total seeds sown? @Lakshmy PJ CUBE
[8/11, 10:35 AM] Arunan HBSCE: What is your objective? @Janhvi Deshmukh When did you set up this trap?
[8/11, 10:37 AM] Arunan HBSCE: How are you sure some pupae are of houseflies?
Please explain @Janhvi Deshmukh
[8/11, 10:37 AM] Arunan HBSCE: How do you expect pupae to interact? @Janhvi Deshmukh
[8/11, 10:38 AM] Arunan HBSCE: What is your objective? @Janhvi Deshmukh
[8/11, 10:39 AM] Janhvi Deshmukh: My objective to trapped the fruit fly and see the Lifecycle of fruitfly…I keep this set on 28/07/201 at 7.30 pm
I trapped flies on 29/07/2021 on 8.30pm
Location:wardha
Date :11/08/2021
Data collector: janhvi deshmukh
Janhvi deshmukh homelab bajaj collage of science wardha
[8/11, 10:41 AM] Janhvi Deshmukh: Actually I see some big pupae and some small pupae …big pupae are red in.colour and size is 4 mm and small pupae some.whitish yellow in colour size is 3 mm and the shape also different of big pupae sir …@Arunan HBSCE sir
Small and Big Pupa @arun
[8/11, 10:44 AM] Janhvi Deshmukh: And also today I seen some housefly hatched …@Arunan HBSCE sir
[8/11, 10:45 AM] Arunan HBSCE: Interesting! @Man Masih Beck RANCHI CUBE
Looking for comments from all!
Let’s develop this idea, critically.
Are we talking about mutations? @Man Masih Beck RANCHI CUBE
@Yash Sheregare CUBE @Aswathy Suresh CUBE @Lakshmy PJ CUBE @Janhvi Deshmukh @Seethalakshmy CUBE @Saida Sayyed and others
[8/11, 10:47 AM] Arunan HBSCE: Any reference, with respect to Nematodes like C elegans and it’s microbiome?
What exactly do we mean by microbiome? @Batul Pipewala Kolkata
[8/11, 10:48 AM] Arunan HBSCE: In what range of microns? What is your assessment and why? @Batul Pipewala Kolkata
[8/11, 10:50 AM] Arunan HBSCE: When do you expect these flies in your trap to complete the Lifecycle?
@Janhvi Deshmukh
[8/11, 10:51 AM] Janhvi Deshmukh: After 4 or 5 days @Arunan HBSCE sir
[8/11, 10:51 AM] Arunan HBSCE: Don’t you expect new flies to emerge within these days?
Why no mention of that? @Janhvi Deshmukh
[8/11, 10:53 AM] Janhvi Deshmukh: I expect 1 or 2 flies emerge in 2 days @Arunan HBSCE sir
[8/11, 10:53 AM] Arunan HBSCE: Photo evidence please! @Janhvi Deshmukh
What happened to Fruitflies?
What is the Lifecycle of Fruitflies in comparison with that of Houseflies?@Janhvi Deshmukh
[8/11, 10:54 AM] Janhvi Deshmukh: I sir today I share some pictures @Arunan HBSCE sir
[8/11, 10:54 AM] Arunan HBSCE: 4 or 5 days after trapping? What is your reasoning? Please describe your reasoning. @Janhvi Deshmukh
[8/11, 10:55 AM] Arunan HBSCE: On what basis do you have this expectation? @Janhvi Deshmukh
[8/11, 10:55 AM] Janhvi Deshmukh: No sir after trapping on 29/07/2021 I think after 4 or five days I see flies @Arunan HBSCE sir
[8/11, 10:56 AM] Arunan HBSCE: Let’s not claim without evidence or without a plausible reasoning. @Janhvi Deshmukh @Aditiya Joshi CUBE @Saida Sayyed
[8/11, 10:56 AM] Janhvi Deshmukh: Actually I see some pupae chage it’s colour @Arunan HBSCE
[8/11, 10:57 AM] Arunan HBSCE: Do you have photo evidence? @Janhvi Deshmukh @Aswathy Suresh CUBE @Yash Sheregare CUBE
[8/11, 10:57 AM] Arunan HBSCE: Any reference to reinforce your argument? @Janhvi Deshmukh @Seethalakshmy CUBE @Saida Sayyed
[8/11, 10:57 AM] Janhvi Deshmukh: Yes sir @Arunan HBSCE
[8/11, 11:12 AM] Seethalakshmy CUBE: Great:+1: @Man Masih Beck RANCHI CUBE
Yes , behavior of both humans are different.
According to this here @Aswathy Suresh CUBE 1year old culture mutation is possible, but i think less mutation compared to 70 year old culture.
We compare with recently traped local flies with her 1 year old culture mutation will happens
[8/11, 11:24 AM] Seethalakshmy CUBE: Sure sir! I will post
[8/11, 11:25 AM] Aswathy Suresh CUBE: 509th day of CUBE ChatShaala: 10th August 2021
41 CUBists from 40 Centres had joined the webinar
INDIA
DELHI
AND College: Aastha Ahuja
Delhi : Garima Kalakoti
Delhi University : Kamakshi Srivastave
New Delhi:Raj Yashwantrao Patil
JHARKHAND
Kokar, Ranchi: Man Masih Beck
Morabadi, Ranchi: Rechel Tirkey
HARIYANA
Panipat : Aashi
KERALA
Thriprayar: Aswathy Suresh
Kandassankadavu : Lakshmy P J
Pazhuvil, Trissur: Seethalakshmi CR
Thanniam, Thrissur: Sidhy PP
MAHARASHTRA
Breach Candy, South Mumbai: Arunan MC
Worli, Mumbai : Isha Pawle
Wardha : Janhvi Deshmukh
Bandra, Mumbai: Saida Sayyed
NES Ratnam College, Mumbai: Khan Shaheen
CHM College, Ulhasnagar : Sakshi Didla
Prabhadevi, Mumbai : Yash Sheregare
TAMIL NADU
PSGR Krishnammal College Coimbatore : Arunima
UTTARAKHAND
Berinag, Pithoragarh: Darshana Joshi
Uttarakhand : Sakshi
UTTAR PRADESH
Noida:Divyanshi Chauhan
kanpur:Heshica Battina Chowdary
Moradabad: Kiran Yadav
Ghaziabad:Shriya varma
VigyanShaala : Neha Yadav,Aakansha Yadav,Aarti Vankatesan,Sachin Ansari,Poonam Yadav,Kanchan Baflia.
WEST BENGAL
Kolkata: Batul Pipewala
•Diksha
•Dr. K. Mohandas
•Mirza Mubarshra
•Nikhel
•Prachi Pant
•R G
•Snehal
•Teki Sandhyasri
•Visheshta Sharma
Note
CUBist(s) who had joined today but their name isn’t included in the list, kindly replied to this message.
Video shared by Janhvi at 11.27am Data collector: janhvi deshmukh
Janhvi deshmukh homelab bajaj collage of science wardha
See some fruitfly pupae some are yellow in colour and one are brown in colour.
Today i see 3 house fly
[8/11, 11:45 AM] Man Masih Beck RANCHI CUBE: Great
Yesterday you had hypothesized that the fruitflies would be emerging out on 11th or 12th August. But instead, housefly emerged out on 11th Aug. What hypothesis can you make after the emergence of houseflies today??[8/11, 12:17 PM] Aswathy Suresh CUBE: Great
@Janhvi Deshmukh. How many old this setup!?[8/11, 12:17 PM] Janhvi Deshmukh: 10 days old @Aswathy Suresh CUBE
[8/11, 12:18 PM] Janhvi Deshmukh: Yes mam but we want fruitfly so I am waiting for fruitfly now @Man Masih Beck RANCHI CUBE
[8/11, 12:19 PM] Aswathy Suresh CUBE: Did you observed today any newly emerged flies in this setup!?
[8/11, 12:21 PM] Janhvi Deshmukh: No on 8 th August I observed one fly emerge aswathy
[8/11, 12:21 PM] Aswathy Suresh CUBE: What is your further plan!?
[8/11, 12:21 PM] Aswathy Suresh CUBE: So, Today how many flies in there!?
[8/11, 12:22 PM] Janhvi Deshmukh: No. Only housefly seen in the box all fruitfly die …
[8/11, 12:47 PM] Aswathy Suresh CUBE: Ooohh… What is your further step in order to trap only fruit flies not other insects!? Is there any plan on your mind!?
Video shared by Shrudiga at 1.06pm
I’ve observed this buttetfly in my home… Looks beautiful… I think they are just roaming around… But looks beautiful 
[8/11, 1:06 PM] +91 97783 22237: Short banded sailer Short banded sailer - Google Search
Its name is Short banded sailer…
[8/11, 1:23 PM] Aswathy Suresh CUBE: Summary : CUBE Home Lab.
Location - Bottukadavu;Thrissur Kerala.
Model System : Fruit Flies.
I Had 4 Single Line Culture in my Place - A, B, C, E
A - Line - 4 Subculture Bottles
SLBK A18, A19, A20, A21
B - Line - 3 Subculture Bottles
SLBK B18, B19, B20
C - Line - 4 Subculture Bottles
SLBK C18, C19, C20, C21
E - Line - 4 Subculture Bottles
SLBK E13, E14, E15, E16.
Date of Subcultures
•28.05.2021 - SLBK A18, B18, C18, E13
•13.06.2021 - SLBK A19, C19, E14
•02.07.2021 - SLBK A20, B19, C20, E15
•19.07.2021 - SLBK A21, B20, C21, E16
Total 15 subculture bottles have.
In this bottles only A21, B19, B20, E15, E16 ; 5 subculture bottles have ~15-20 Flies, ~10-15 Larvae, 10-20 Pupae present.
SAD BREAKING NEWS happened in Last month July was I lost C culture completely 
I tried to revive them back but i couldn’t save in emergency time (Because I had exams & Other things need to done in last week around August 1st) so I couldn’t done anything for to save these C Line cultures.
Currently I have A - Line, B - Line, E - Line.
A - Line : also in trouble, because three only one fly is alive. Hope can revive that culture also.
B - Line : Reasonable number of Flies, Larvae, Pupae have in B culture.
E - Line : Also have Reasonable number of Flies, Larvae, Pupae have Present in E culture.
All sub culture bottles I can able to see Microworms…
(No worry about these cultures).
Summary By Aswathy Suresh CUBE SN Nattika
Collaborators : @Arunan HBSCE sir @Yash Sheregare CUBE @Isha Pawle @Ishita Assam @Janhvi Deshmukh @Rechel Turkey Cube Ranchi Moina @Aastha Ahuja Cube @Man Masih Beck RANCHI CUBE @KIRAN CUBE @Batul Pipewala Kolkata
video shared by Batul Pipewala at 2.59pm
Nematodes observed using mobile phone and magnifying glass of 10X at Homelab Kolkata BatulP 11/8/21
[8/11, 3:40 PM] Aswathy Suresh CUBE: Iam expecting using this fly I can revive back my A line culture. I still have a hope from this one fly.
In A21 Bottle I can’t able to see more eggs,larvae, pupae in there. Only this one fly is left inside culture.
[8/11, 3:43 PM] Aswathy Suresh CUBE: Media of the culture is Quite good. There is no Liquidy or water condensation can’t able seen yet
[8/11, 3:44 PM] Aastha Ahuja Cube: Are there any larvae or pupa in c culture bottle ?
Can you take a closer look at those bottles ?remember you thought you lost b line on 13 June but you found larvae in it on 24June , is it possible that we can get such thing in C bottles also don’t loose hope search for something in those bottles …
When did you actually loose C line?
Why are we not preparing replicate bottle is it not possible to create replicates ?
[8/11, 3:49 PM] Priti Kanade: To verify Man’s hypothesis
[8/11, 3:49 PM] Priti Kanade: By carrying out olfaction experiment
1 - Ctrl WT
2- Csbz
3- One year old lab culture
With apt replicates for each of them
[8/11, 3:50 PM] Aswathy Suresh CUBE: Actually, I lost C culture on 22.07.2021.
[8/11, 4:00 PM] Aswathy Suresh CUBE: I transferred 2 flies from C18 culture to C21 culture bottle on 19.07.2021.
SLBK C21
Date ways observed ;
•18.07.2021 - Made TRSV media
•19.07.2021 - Transferred 2 flies from C18 (28.05.2021 subculture) to C21(new media bottles).
•20.07.2021 - Media Normal (No water, No liquidly, No Condensation).
•21.07.2021- Started to observed Condensation around bottle wall. 2 Flies were alive.
•22.07.2021 - media bottom became started to present bubbles, 2 Flies where dyed.
This was happened 
. @Aastha Ahuja Cube
[8/11, 4:02 PM] Aastha Ahuja Cube: What about c 20 ?
[8/11, 4:02 PM] Aastha Ahuja Cube: Were those 2 flies gravid females
[8/11, 4:03 PM] Aswathy Suresh CUBE: Yes, I did made subculture for to save C - Line flies. Because that time I don’t have any C line flies to transfer in new media bottles. I was waiting for to emerging new flies from C18 bottle.
[8/11, 4:22 PM] Aswathy Suresh CUBE: C 20 Culture there was no flies.
SLBK C20
Date ways observation
•01.07.2021 - Made TRSV Media.
•02.07.2021 - Transfer 30 flies from C19 (13.06.2021 subculture) to C20.
•03.07.2021 - Total 5 flies were Dyed, 15 flies alive. Media perfectly ok, there is no water condensation, No water condensation.
04.07.2021 - I didn’t check these bottle.
•06.07.2021 - Observed total (8+5 flies Dyed) flies dyed. 17 alive. Media had no water condensation, solidified conditions.
•07.07.2021 - No observation
•08.07.2021 - Total flies 12 flies alive. Remaining 18 flies were died. 2 small larvae (1st instar) I observed in side culture bottle.
•10.07.2021 - There is no flies completely dead inside culture. But tgere no water condensation, media had no problem.
Expected some eggs, larvae would be there. But after I didn’t get any flies from this C20 culture bottle.
[8/11, 4:23 PM] Aswathy Suresh CUBE: No, One was female & another one was male.
[8/11, 4:45 PM] Lakshmy PJ CUBE: I got it from cardamine plants at home.
Initially I collected cardamine plants from plant nursery
[8/11, 4:50 PM] Lakshmy PJ CUBE: Long term objective -Floral dip method
Short term objective -
- To observe life cycle
- observing heteroblasty
3)Identifying cardamine plants
Seeds collected -25/7/2021
No of seeds on one seed pod-19
Date of sawing Cardamine seeds- -25/7/2021
Date:30/7/2021
Seeds germinated( cotyledonary stage)-13
Date:31/7/2021
Seeds germinated (cotyledonary stage)-15
Date:6/8/2021
Seeds germinated-17
Cotyledonary stage+ one simple leaf arised
Date:10/8/2021
Seeds germinated-17
Cotyledonary stage+ two simple leaf arised
@Seethalakshmy CUBE @KIRAN CUBE @Arunan HBSCE
[8/11, 4:52 PM] Lakshmy PJ CUBE: Out of 19 seeds 17 seeds germinated.
Percentage of seed germination - 89%
@Arunan HBSCE @Seethalakshmy CUBE
[8/11, 4:52 PM] KIRAN CUBE: Beautiful Cardamine, how have you been maintaining Cardamine in your Home Lab as @Seethalakshmy CUBE is facing some issues in getting good culture of Cardamine.
Please tell us your method of maintaining these beautiful Cardamine
[8/11, 4:53 PM] Arunan HBSCE: Please give a short summary of your work so far so that all can follow. @Janhvi Deshmukh
[8/11, 4:54 PM] KIRAN CUBE: Tell us the whole story from the beginning how you have harvested seeds? What type of seeds did you sow?
[8/11, 4:54 PM] Arunan HBSCE: Please give us a short summary for us to make any comment. @Janhvi Deshmukh
Hina Mudgal:
[8/11, 4:55 PM] KIRAN CUBE: @Lakshmy PJ CUBE how do we indentify species of Cardamine?.
[8/11, 4:57 PM] KIRAN CUBE: Ohhh are these eggs of mosquito? Which mosquito’s eggs?
[8/11, 4:58 PM] Hina Mudgal Kanpur CUBE: MOSQUITO eggs trapped, in ovitrap day 3.
Set up by vyom gupta class 9.
Objective to identify the stages of lifecycle of MOSQUITO.
Dated - 11.08.21
[8/11, 4:59 PM] Hina Mudgal Kanpur CUBE: We are identifying the type of MOSQUITO @ hina cube kanpur.
[8/11, 5:04 PM] Janhvi Deshmukh: On 28/07/201 at 7.30 pm I kept the setup on my backyard near the dishwashing area on 29/07/201 at 8.30 pm I got 13 flies I close the box with tip after that on 01/08/2021 I see some larve moiving inside the bait on 02/08/2021 I seen some big larve which is 4 mm in size some are 3 mm in size on 4 rth August I seen some pupue some are whitish yellow in colour size 3mm and some are red in colour size 4 mm I seen some 6,7 fruitfly pupae and 10 to 13 housefly pupae on 7 aug I see one fruitfly emerged I think due to water condensation they die today I see some housefly emerge now I am waiting for fruitfly …
Location:wardha
Data collector: janhvi deshmukh
Janhvi deshmukh homelab bajaj collage of science wardha
[8/11, 5:05 PM] Arunan HBSCE: In how many days?
[8/11, 5:10 PM] KIRAN CUBE: Invitation to join today CUBE chatShaala
PLEASE ADD NAME OF PLACE/AFFILIATION ALONG WITH THE NAME WHILE JOINING THE WEBINAR
Here’s the direct link of the CUBE chatShaala webinar: https://webinar.hbcse.tifr.res.in/b/kir-vgc-6jf
Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
[8/11, 6:33 PM] KIRAN CUBE: Excellent discussion is going on in CUBE chatShaala around Cardamine which gives us great context to understand Genetic Engineering much better by just handling this weed we can understand how a foreign gene can be incorporated in plants.
Please join😇
Seethalakshmi:
𝗪𝗵𝗮𝘁 𝗛𝗮𝗽𝗽𝗲𝗻𝘀 ???
𝗘𝘅𝗽𝗲𝗿𝗶𝗺𝗲𝗻𝘁
𝐅𝐨𝐫 𝐞𝐱𝐩𝐞𝐫𝐢𝐦𝐞𝐧𝐭𝐚𝐭𝐢𝐨𝐧
𝐍𝐞𝐜𝐞𝐬𝐬𝐚𝐫𝐲 𝐒𝐮𝐩𝐩𝐥𝐢𝐞𝐬 :
*3 small beakers.(20 ml)
- Milk
- Vinegar
- Curd
𝐒𝐭𝐞𝐩𝐬 :
After heating the milk, let it cool for 1 hour.
Take 3 small beakers and add 5 ml of milk each small beakers
In all beaker
Add 2.5𝐦𝐥 vinegar to Beaker 𝗔.
Beaker 𝗕 - Only 5𝐦𝐥 milk.
Beaker 𝗖 - add 2.5 𝐦𝐥 Curd to 5 𝐦𝐥 of milk .
*To observe each hour.
- Beaker 𝗔 : 𝑽𝒊𝒏𝒆𝒈𝒂𝒓 (2.5𝐦𝐥) + 𝑴𝒊𝒍𝒌(5𝐦𝐥)
*Beaker 𝗕 : 𝑶𝒏𝒍𝒚 𝒎𝒊𝒍𝒌(5𝐦𝐥) - Beaker 𝗖 : 𝑪𝒖𝒓𝒅(2.5𝐦𝐥)+ 𝑴𝒊𝒍𝒌 (5𝐦𝐥).
𝐄𝐱𝐩𝐞𝐜𝐭𝐚𝐭𝐢𝐨𝐧 :
All of them become Curd.
Only Beaker C we get True curd. Others are false Curd.
Location : Pazhuvil, Thrissur, Kerala.
Date of setup : 11/8/21.
Time: 5:20 pm.
Data collector : Seethalakshmi CUBE Nattika.
Seethalakshmi:
Batul Pipewala:
Nematodes growing on rava sugar are smaller in size approx 1mm compared to the ones growing on raw potato approx 2mm size.Cube Homelab Kolkata BatulP
Seethalakshmi:
After 1 hr
Beaker𝗔: (𝒗𝒊𝒏𝒆𝒈𝒂𝒓 +𝑴𝒊𝒍𝒌 )
I can see some precipitate .
Time:6:20 pm
Date of photo: 11/8/2021
Location : Pazhuvil.
Data collector: Seethalakshmi.C.R. CUBE
Batul Pipewala:
New medium prepared as rava sugar medium is developing fungus after 6days.This time to half spoon of rava and 1/4th spoon of sugar which is boiled with half cup water ,two drops of vinegar is also added.Nematodes will be transferred from old rava medium to this fresh medium.11/8/21Cube Homelab Kolkata BatulP
Seethalakshmi:
After 1 hr
Beaker B
Only Milk
After 1 hr
Beaker B
Curd+milk
[8/11, 7:21 PM] Seethalakshmy CUBE: @Arunan HBSCE sir,@KIRAN CUBE ma’am,@Batul Pipewala Kolkata ma’am, @Jai Kishan CUBE ,@Rechel Turkey Cube Ranchi Moina ,@Aswathy Suresh CUBE ,@Janhvi Deshmukh ,@Aditiya Joshi CUBE , @Man Masih Beck RANCHI CUBE And others
[8/11, 8:11 PM] KIRAN CUBE: Somebody ended the CUBE chatshaala by mistake, i m restarting it please join back everyone
[8/11, 8:12 PM] Seethalakshmy CUBE: Ok ma’am
[8/11, 8:13 PM] KIRAN CUBE: Please join back everyone
[8/11, 8:25 PM] Arunan HBSCE: Joined.
[8/11, 8:49 PM] Dr. Sarita kumar Delhi CUBE: Am unable to join🤔
[8/11, 8:49 PM] Janhvi Deshmukh: Mam try to join
and the upper region of the plant 
…And this color varies from dark green to Light reddish-brown…Why does this vary from plant to plant 
@Hina Mudgal Kanpur CUBE
please share your experiment with us 
@Aswathy Suresh CUBE Let’s expect some visible larvae in 2 days!
wow what is your next plan 
wow !what they do in hibiscus 
…so it’s a phylluntus amarus … Janhvi deshmukh homelab bajaj collage of science wardha maharshtra
Here’s the direct link of the CUBE chatShaala webinar: 
No of Unhatched Pupae : ~40
This video is so amazing! We can see the white stripes on the back of ants.
Date:- 19/08/2021, Time: 2.15pm, Location:- Ulwe, Navi Mumbai, Maharashtra.
oxalis
@Shahin:maple_leaf:
on Cardamine plant:seedling: in 2nd right side patch of Cardamine forest in front of HBCSE main building.
was emerged.
for Psyche butterflies.
with these characteristics that you think are morphological features.
legs quite visible?
1)Involved in Discussion
Water:cup_with_straw:( 150 ml)
(~50 gm)
Rava:rice:(20 gm)
(15 gm)
Remove the bottle and we can see flies getting immobilise.
?
species, right 
_agariplaadil..JPG)
and Ask Why tomorrow!
In yesterday’s chatshala, we started discussing with the microscopic picture which was shared by 
, like from tap water using nutrient rich coconut water. 
