16th August 2021, 9.30pm to 17th August 2021, 9.30pm
[9:23 PM, 8/16/2021] Ankit Yadav Xms Work Shop Cube Patkar College: This paper is regarding imaginal discs with special reference to fruit fly
Imaginal disc r sac like structure found inside larva of insects that undergo metamorphosis.
These discs decide external structures such as head, thorax, limbs and genitalia.
It is still a mystery that how these discs decides fate of an insect. The reason imaginal discs are of such interest to developmental biologist is that it provide a powerful model to study multiple aspects of biological mechanism underlying diseases.
[9:34 PM, 8/16/2021] Seethalakshmy CUBE: Crazy Ants
[10:17 PM, 8/16/2021] Aswathy Suresh CUBE: 515th day of CUBE ChatShaala: 16th August 2021
24 CUBists from 24 Centres had joined the webinar.
INDIA
DELHI
DSC, Delhi : Dr P. Chitralekha
GOA
Goa : Diksha Gaunker
JHARKHAND
Kokar, Ranchi : Man Masih Beck
KERALA
Thriprayar : Aswathy Suresh
Pazhuvil, Thrissur : Seethalakshmi CR
Palakkad : Shrudhiga
Thanniam, Thrissur : Sidhy PP
Alapad, Thrissur : Vishnupriya CJ
MAHARASHTRA
Kalyan : Aditya Joshi
Breach Candy, South Mumbai : Arunan MC
Worli, Mumbai : Isha Pawle
Govandi, Mumbai : Rafikh Shaikh
Titwala : Ritik Baviskar
Dahanu Road, Palghar : Sachin Pradhan
Panvel : Shraddha Sonavane
NES Ratnam College, Mumbai : Sunita Chahar
Prabhadevi, Mumbai : Yash Sheregare
TAMIL NADU
PSGR Krishnammal College Coimbatore : Arunima
UTTAR PRADESH
Moradabad : Kiran Yadav
Uttar Pradesh : Ankit Yadav
WEST BENGAL
Kolkata : Batul Pipewala
: C L A B
: Deeksha pandey
: Prachi Pant
Note
Cubist(s) who had joined today but their name isn’t included in the list, kindly replied to this message.
[10:57 PM, 8/16/2021] Lydia cUBe Drosphila: Good!
Please post pictures and videos of these cultures. Looking forward to it.
[11:03 PM, 8/16/2021] Lydia cUBe Drosphila: You might be having dates of all those cultures bottles.
We’ll track each bottle one by one
For eg. If B is the bottle in which your flies died, then write a post here of the B bottle specifically with date of transfer of flies into B bottle, number of flies transferred, bottle name from which the flies where transferred (suppose A) into B bottle, then dates of when larvae, pupae were seen (if seen) in bottle B.
Similarly, write the history of bottle A as well from which the flies were transferred into B.
[11:10 PM, 8/16/2021] Lydia cUBe Drosphila: Okay…yes that is a problem
How many culture bottles do you have now?
Please give the names of your culture bottles one by one with date of transfer of flies of that bottle + number of flies in that bottle (females, males, gravid)
[11:16 PM, 8/16/2021] Lydia cUBe Drosphila: Yes 
I had a doubt❓
Tell me what you think 
Can we call a plant that we study a model organism? Or would it be called a model system? Drosophila, Moina, mosquito, snails can be called model organisms, but what about Phyllanthus, Cardamine? What do you think? Will there be a difference in the terms model organism and model system in this case?
[11:23 PM, 8/16/2021] Lydia cUBe Drosphila: Does this happen all the time? If a change in the original structure of a protein occurs, is this change always irreversible?
[11:27 PM, 8/16/2021] Lydia cUBe Drosphila: 
What if you keep a potato slice just like that without spreading the fermented coconut water on it? What do you expect? Do you plan to keep a potato slice as a control?
[11:28 PM, 8/16/2021] Aditiya Joshi CUBE: Yes
[11:29 PM, 8/16/2021] Aditiya Joshi CUBE: A non inoculated slice
VIDEOS SHARED BY ASWATHY AT 12.49 AM
[12:06 AM, 8/17/2021] Aswathy Suresh CUBE: Example of 46 days old bottles - SLBK E15
started date of culture : 02.07.2021
17/08/2021
No of Flies : ~60
No of Pupae : ~150
No of Unhatched Pupae : ~40
No of Hatched Pupae : ~110
No of Dead Flies : ~50
Condition of media : Solidified.
Location : Bottukadavu - Thrissur Kerala.
[12:13 AM, 8/17/2021] Aswathy Suresh CUBE: 29 Days old culture bottle - SLBK E16
started date of culture : 19.07.2021
17/08/2021(today)
No of Flies : ~60-65
No of Pupae : ~120
No of Unhatched Pupae : ~40
No of Hatched Pupae :
~80
No of Dead Flies : ~30
Condition of media : Solidified.
Location : Bottukadavu - Thrissur Kerala.
[12:14 AM, 8/17/2021] Seethalakshmy CUBE: No @Lydia cUBe Drosphila
The body strictly regulates pH and temperature to prevent proteins such as enzymes from denaturing.
Some proteins can refold after denaturation while others cannot.
The reference says some protiens can re - gain it’s previous str.
[12:25 AM, 8/17/2021] Seethalakshmy CUBE: Good question @Abhijeet Singh
Yeah we can look their morphological features.
That’s actually iam trying to find.
What got from references
Back crazy ants have
Non- clubed antenna
Size:2.3- 3 mm( worker)
And their movement ( random movement )
[12:31 AM, 8/17/2021] Aswathy Suresh CUBE: SLBK A22
subculture started date : 12.08.2021
Update : Fruit Flies
Transfer Flies : 1 Fly (Look Like Gravid One)
Expectations :
16.08.2021 : ~30 Eggs + ~20 Larvae + ~10 Pupae (on 5th day expectations)
Observation : 5th Day
17/08/2021
No of Eggs : ~5
No of Larvae : 0
No of Pupae : 0
No of Flies : 1 (Transferred one)
Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
[12:36 AM, 8/17/2021] Aswathy Suresh CUBE: SLBK B21
subculture started date : 12.08.2021
Update : Fruit Flies
Transfer Flies : 12 Flies
Gravid Flies : 5, Male Flies : 7
Expectations :
16.08.2021 : ~60 Eggs + ~35 Larvae + ~30 Pupae (on 5th day expectations)
Observation : 5th Day
17/08/2021
No of Eggs : ~40
No of Larvae : 20
No of Pupae : 30
No of Flies : ~2 (Transferred flies)
Dead Flies : ~10
Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
[12:40 AM, 8/17/2021] Aswathy Suresh CUBE: SLBK E17
subculture started date : 12.08.2021
Update : Fruit Flies
Transfer Flies : 14 Flies
Gravid Flies : 6, Male Flies : 8
Expectations :
16.08.2021 : ~70 Eggs + ~40 Larvae + ~30 Pupae (on 5th day expectations)
Observation : 5th Day
17/08/2021
No of Eggs : ~30
No of Larvae : 0
No of Pupae : 0
No of Flies : ~14 (Transferred flies)
Dead Flies : 0
Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
[12:46 AM, 8/17/2021] Aswathy Suresh CUBE: Fruit flies eggs can able see in the above part of media, bottle wall sides. Video taken from SLBK E17 (subculture bottle). Date of video 16/08/2021
VIDEO SHARED BY SEETHALAKSHMY AT 12.54PM
[12:54 AM, 8/17/2021] Seethalakshmy CUBE: Update on Ant model system.
Inside my formicarium again i can see some pupae( small white ant)as well as larvae( a cylindrical shape with black dot).
Ants carrying their own larvae and pupae.
Location : Pazhuvil, Thrissur, Kerala.
Date :16/8/21.
Time :11:41 pm.
Data collector : seethalakshmi. C. R. CUBE Nattika
[1:01 AM, 8/17/2021] Seethalakshmy CUBE: A few ants hold larvae and pupae inside the formicarium and a few outside. 
Location : Pazhuvil
Date:16/8/21
Time:11:43 pm
Data collector: Seethalakshmi
[1:05 AM, 8/17/2021] Man Masih Beck RANCHI CUBE: 
is there a way you could remove the larvae from the colony to observe the different larval stages more closely?
How many larval stages do your ants have @Seethalakshmy CUBE ?
[8:07 AM, 8/17/2021] Lakshmy PJ CUBE: Heteroblasty is the condition in which plants show a certain degree of variation among individual metamers during ontogeny. In some cases, however, there are abrupt and substantial changes in form and function (e. g. in leaf form, leaf size, phyllotaxy, internode length, anthocyanin pigmentation, rooting ability, or wood structure). These plants were called “heteroblastic” by Karl Goebel more than a century ago, but the functional significance of this type of ontogenetic change, the evolutionary trajectories in different plant groups, even their frequency in the plant kingdom are still unresolved issues. We argue that slow progress is partly due to an on-going terminological
confusion and the lack of distinction between other developmental processes such as ontogenetic drift.
https://www.researchgate.net/publication/226433716_Heteroblasty-A_Review
@Aditiya Joshi CUBE @Arunan HBSCE @KIRAN CUBE @P. Chitralekha Cube JNU @Seethalakshmy CUBE
[8:27 AM, 8/17/2021] Seethalakshmy CUBE: Figure - PMC
[8:34 AM, 8/17/2021] Seethalakshmy CUBE: I got this from above reference. But this is life cycle of camponotus ant. But didn’t get crazy-ants life cycle
[9:56 AM, 8/17/2021] Seethalakshmy CUBE: 𝗦𝘂𝗺𝗺𝗮𝗿𝘆 𝗼𝗳 515𝘁𝗵 𝗱𝗮𝘆 𝗼𝗳 𝗖𝗨𝗕𝗘 𝗖𝗵𝗮𝘁𝘀𝗵𝗮𝗮𝗹𝗮 .
Yesterday we started the discussion 𝗔𝘀𝘄𝗮𝘁𝗵𝘆’𝘀 fruitflies. She trying to bulid up olfactometer in her 𝗛𝗼𝗺𝗲 𝗟𝗮𝗯 .
As well as we talk about
𝗖𝗵𝗮𝗶 𝗮𝗻𝗱 𝘄𝗵𝘆 ?
- 𝗖𝗵𝗮𝗶 𝗮𝗻𝗱 𝘄𝗵𝘆 ?" is jointly organised by TIFR and Prithvi Theatre, Juhu. It is a monthly forum to enable informal discussions of interesting scientific issues outside a traditional academic setting.
we discussed about Ants model system.
𝗦𝗲𝗲𝘁𝗵𝗮𝗹𝗮𝗸𝘀𝗵𝗺𝗶 raised a question :The olfactometer only for fruitflies larvae? We can use this setup for other organism like Ants, snails?
So we want to check the life cycle of Ants.
Life cycle of Ants
Egg-larvae - pupae - adult
Which type of larval stage we can suggest for this experiment??
Then seethalakshmi tell about her Home Lab.
We need to find which types of ants she culture her 𝗛𝗼𝗺𝗲 𝗟𝗮𝗯 ?
How can identify an Ant species?
What are Morphological features of Ants???
Yes, we will continue the discussion…
please give your inputs
@Aswathy Suresh CUBE ,@Man Masih Beck RANCHI CUBE ,@Arunan HBSCE sir,@KIRAN CUBE ma’am,@Sunita Chahar CUBE ma’am,@Batul Pipewala Kolkata ma’am,@P. Chitralekha Cube JNU ma’am,@Shraddha Sonavane CUBE ,@Aditiya Joshi CUBE and others …
[10:48 AM, 8/17/2021] Abhijeet Singh: So,
We actually did a year around mosquito project.
We were 8-10 students from 7-9th grade. We used to catch mosquito at our school together (before and after school hours) and at our homes by clap-trap method.
We used to collect the mosquito in a small container or box and the next day we put all that mosquitoes on a black paper. So that we can distinguish between Aedes and non-aedes ones(as aedes mosquito has white stripes on their legs)
We take the total count and and aedes mosquitoes number in that. We did this whole year with the hypothesis that we will fine more number of aedes in the late monsoon months(August to September end and initial weeks of October maybe)
Basic idea behind our hypothesis was that as after rain the mosquitoes will get more…
[10:53 AM, 8/17/2021] Abhijeet Singh: I did look for pictures if there’s any in my backup or mail but I couldn’t find. I’ll try to show that if I get any reference or something.
[11:00 AM, 8/17/2021] Shivam CUBE NEMATODE: Do share White bord screenshots from yesterday’s discussion.
[11:00 AM, 8/17/2021] Abhijeet Singh: Looking forward updates from @Hina Mudgal Kanpur CUBE Ma’am .
Please let us what do you think of this everyone!!!
REFERENCE
Yesterday discussed topics related important reference given in public chats.
Chai & Why
TIFR’s unique outreach initiative since 2009 has been the “Chai and Why?” series, a forum to enable informal discussions of interesting scientific issues outside a traditional academic setting in a Science Café format.
http://univ.tifr.res.in/gs2021/Gallery/Images/022-Canteen.JPG
Light Receptors In Fruit Fly Larvae
The larval eye is situated anteriorly and lateral, at the tip of the larva,Larval eye of drosophila - Google Search
[11:28 AM, 8/17/2021] Seethalakshmy CUBE: thank you @Aswathy Suresh CUBE for giving white boards
[11:32 AM, 8/17/2021] Aswathy Suresh CUBE:
[12:17 PM, 8/17/2021] Abhijeet Singh: Actually that’s great point to look into this more clearly!
I think that if we go definition wise…
In biology ‘system’ is something like a group of things working together to make stuff happen that helps in growth, nutrition and reproduction.
‘organism’ is any living structure, can be plant or fungi or bacteria capable of growth and reproduction.
Drosophila, Moina, mosquito, snails can be called model organisms, but what about Phyllanthus, Cardamine?
I haven’t categorised then into ‘model system and organism’ So if your trying to convey that they are seperate please let me know how and why do you think that!?
Can we call a plant that we study a model organism? Or would it be called a model system?
I think we can call it both . Plants have differe…
[12:42 PM, 8/17/2021] Abhijeet Singh: Photos amd references will do great if you can share them with us!!
@Janhvi Deshmukh
[12:45 PM, 8/17/2021] Abhijeet Singh: So why fermented coconut water is important @Aditiya Joshi CUBE .
What ingredients do yeast or bacteria or fungii need to grow?
What is the basic idea behind fermentation!?
[12:49 PM, 8/17/2021] Abhijeet Singh: Yeahhh I was also thinking about it!!
It would be great!
But proper understanding and references in this field is what we require before. Lets tune in Chatshaala for discuss on it!! @Lydia cUBe Drosphila @Aditiya Joshi CUBE @Saida Sayyed
[12:52 PM, 8/17/2021] Abhijeet Singh: With a brush or something it would be easy to pick them up and transfer!!
@Batul Pipewala Kolkata
What do you think?
And also what are we studing in nematodes?
Can you give a context about it?
[1:21 PM, 8/17/2021] Aditiya Joshi CUBE: Actually, the traditional toddy is made from palm or coconut. There the juice/sap of palm/coconut flower. That is collected and fermented to give an alcoholic drink toddy.
About fermentation what I think is, the sugars like glucose will be converted to pyruvic acid. This pyruvic acid then will be converted into alcohol.
Yeasts/bacteria will require sugar or a carbon source, nitrogen source some ions like sodium, potassium, chloride, etc.
So, I think, the yeast/bacteria will utilise these nutrients and result in formation of alcohol.
I will try to observe the growth of these yeast/bacteria on a boiled potato slice.
[1:21 PM, 8/17/2021] Aditiya Joshi CUBE: @Abhijeet Singh
[1:37 PM, 8/17/2021] Arunan HBSCE: What do we mean by “internal interactions”?
[1:39 PM, 8/17/2021] Arunan HBSCE: How are we sure this is an insect? @DP HBCSE
PHOTO SHARED BY ADITIYA JOSHI AT 1.41PM
[1:41 PM, 8/17/2021] Aditiya Joshi CUBE: Ivy gourd vine extending ‘hands’ to grasp the support… We can see tip of tendril bit curved to creat a small hook like thing… Kala Talav, Kalyan west. 16/8/2021. 9:30 pm
[1:42 PM, 8/17/2021] Arunan HBSCE: When we boil, why egg white becomes hard?
That way, even egg yolk too becomes hard, on boiling, right? Doesn’t it? @Seethalakshmy CUBE @Aditiya Joshi CUBE
[1:47 PM, 8/17/2021] Arunan HBSCE: How are we sure this is Psyche caterpillar? @Arunima Dey Aren’t other caterpillars possible in Cardamine?
[2:04 PM, 8/17/2021] Seethalakshmy CUBE: Yes sir
[2:05 PM, 8/17/2021] Arunan HBSCE: An old CUBE saying
Confusion is the starting point of understanding. What do you say? @Seethalakshmy CUBE
[2:06 PM, 8/17/2021] Arunan HBSCE: Examples? @Seethalakshmy CUBE
[2:06 PM, 8/17/2021] Seethalakshmy CUBE: But iam not sure the egg yold contains protein like albumin.
[2:07 PM, 8/17/2021] Seethalakshmy CUBE: Sure sir, I agree to this.
[2:14 PM, 8/17/2021] Arunan HBSCE: As Lydia wants to know, what do you expect in this Control slice? @Aditiya Joshi CUBE
[2:17 PM, 8/17/2021] Arunan HBSCE: Ooooh! No larvae mentioned! 
@Aswathy Suresh CUBE
[2:19 PM, 8/17/2021] Seethalakshmy CUBE: Yes sir, the egg yolk contains protein but at same time it contains lots of fat.
[2:20 PM, 8/17/2021] Arunan HBSCE: Interesting. Don’t you expect any larvae?
Aren’t you surprised?
@Aswathy Suresh CUBE And do we still call it a healthy culture? @Aswathy Suresh CUBE @Lydia cUBe Drosphila @Yash Sheregare CUBE @Sidhy Kerala Drosophila @Man Masih Beck RANCHI CUBE @Aastha Ahuja Cube
[2:20 PM, 8/17/2021] Batul Pipewala Kolkata: can we remove the larvae and pupae and click their pics?Can we collect the pupae in a separate bottle and observe its metamorphosis
[2:22 PM, 8/17/2021] Arunan HBSCE: No larvae mentioned? Why? How can we call as healthy culture? @Aswathy Suresh CUBE
[2:27 PM, 8/17/2021] Arunan HBSCE: How about photo evidence of eggs? @Aswathy Suresh CUBE All are very eager to see.@Aswathy Suresh CUBE
VIDEO SHARED BY BATUL AT 2.28PM
Dense Nematode culture on raw potato slice started on 6thAug,eleven days old.On first day 3to4 drops of milk was added and nematodes transferred from original potato slice that had soil.After that only two drops of water added everyday.Cube Homelab Kolkata BatulP 17/8/21
[2:32 PM, 8/17/2021] Arunan HBSCE: Congrats! 
@Aswathy Suresh CUBE This is the stock culture you saved because of collaborative interaction and help from others. @Aswathy Suresh CUBE Please comment.
[2:38 PM, 8/17/2021] Arunan HBSCE: What about at least two of the latest SLBK C bottles? @Aswathy Suresh CUBE
Every one is eager. In fact please post state of all SLBK C bottles. @Aswathy Suresh CUBE
What do you say? @Lydia cUBe Drosphila @Yash Sheregare CUBE @Aastha Ahuja Cube @Raj Yashwantrao Patil CUBE @KIRAN CUBE
[2:40 PM, 8/17/2021] Arunan HBSCE: Why are the ants going out?@Seethalakshmy CUBE Look at 9 seconds.
[2:42 PM, 8/17/2021] Arunan HBSCE: Are you sure pupae will look like white ants? Any reference? @Seethalakshmy CUBE
[2:44 PM, 8/17/2021] Arunan HBSCE: Please give all essential details of which ant, Date, Time, Location of CUBEHomeLab, Name of Data collector along with each photo. @Seethalakshmy CUBE
[2:47 PM, 8/17/2021] Arunan HBSCE: More confusing!Please describe in your own words and of course with reference link. @Lakshmy PJ CUBE
What is the Context? @Lakshmy PJ CUBE
[2:47 PM, 8/17/2021] Arunan HBSCE: More confusing!Please describe in your own words and of course with reference link. @Lakshmy PJ CUBE
What is the Context? @Lakshmy PJ CUBE
[2:48 PM, 8/17/2021] Arunan HBSCE: Please explain in your own words, in a simple way as you understood it. @Seethalakshmy CUBE
PHOTO BY BATUL AT 2.49PM
[2:49 PM, 8/17/2021] Batul Pipewala Kolkata: No nematode growth on Rava sugar vinegar medium started on 11/8/21 Though I had reduced the vinegar in the medium nematodes did not multiply.17/8/21
[2:50 PM, 8/17/2021] Arunan HBSCE: Please ask for help, very openly, showing where are you stuck? @Seethalakshmy CUBE
[2:54 PM, 8/17/2021] Arunan HBSCE: What is Clap trap method? @Abhijeet Singh
[2:57 PM, 8/17/2021] Arunan HBSCE: Great! @Abhijeet Singh Which year you did this work? Please post some photos, as evidence, from your presentation etc. @Abhijeet Singh Please contact your collaborators too.
@Hina Mudgal Kanpur CUBE Please join in discussion.
[3:00 PM, 8/17/2021] Arunan HBSCE: Wasn’t there some different result and different conclusion came from others in your own school? @Abhijeet Singh
[3:01 PM, 8/17/2021] Arunan HBSCE: Please post some evidence. Please ask your collaborators to post to you too. @Abhijeet Singh
[3:01 PM, 8/17/2021] Arunan HBSCE: How about contacting your collaborators? Who were your collaborators? @Abhijeet Singh
[3:02 PM, 8/17/2021] Batul Pipewala Kolkata: The question arises how nematodes are surviving in @Aswathy Suresh CUBE medium TRSV? Is tomato necessary?
Glimpses of CUBE History CUBE Feb2018 HBCSE, TIFR, Mumbai
[3:12 PM, 8/17/2021] Arunan HBSCE: What is Imaginal disks? Are they imaginary? Are Imaginal disks different from larval sense organs and larval brain? @Aswathy Suresh CUBE @Seethalakshmy CUBE
[3:17 PM, 8/17/2021] Arunan HBSCE: Please comment on topics to continue discussion for othetsto understand and for us to get more clarity.
@Seethalakshmy CUBE
[3:17 PM, 8/17/2021] Arunan HBSCE: others*
[3:25 PM, 8/17/2021] Aswathy Suresh CUBE: Larvae can’t able to see inside E15 culture bottle, Bottle full covered with fruit flies pupae shell sir, so can’t able to seen them.
[3:27 PM, 8/17/2021] Aswathy Suresh CUBE: Larvae can’t able see, but lot of unhatched pupae I observed inside culture ~40. That means still some eggs,larvae also there in the culture.
[3:31 PM, 8/17/2021] Aswathy Suresh CUBE: Yes, This bottle can called healthy culture because After 46 days later still have Larvae, Unhatched Pupae Adult Flies also there. Approximately 60 Adult flies were present inside the culture. Media have no contamination, still solidified condition.
[3:35 PM, 8/17/2021] Aswathy Suresh CUBE: Most of the Larvae were present inside the media portion… That’s why I couldn’t able to count them. Approximately 20 more larvae I observed yesterday.(sorry for not mentioning larvae).
[3:45 PM, 8/17/2021] Arunan HBSCE: Don’t we have expectations of larvae in a healthy bottle brimming with flies? @Aswathy Suresh CUBE
So, when not located, as against our expectation, should we not be worried and made it as a BREAKING NEWS STORY?
What’s your comment? @Aswathy Suresh CUBE @Lydia cUBe Drosphila @Yash Sheregare CUBE @Man Masih Beck RANCHI CUBE @Raj Yashwantrao Patil CUBE @Sachin Pradhan CUBE @Aastha Ahuja Cube
[3:46 PM, 8/17/2021] Batul Pipewala Kolkata:
[3:46 PM, 8/17/2021] Arunan HBSCE: Incidentally, in this bottle SLBKE16, I was able to locate larvae at 11 seconds into the video. @Aswathy Suresh CUBE
[3:49 PM, 8/17/2021] Batul Pipewala Kolkata: Yes sir I could see 5 larvae moving in this bottle
[3:50 PM, 8/17/2021] Arunan HBSCE: Aby examples? @Seethalakshmy CUBE
What kind of protein in Casein compared to Globin? How do we describe it? @Seethalakshmy CUBE
[3:50 PM, 8/17/2021] Aswathy Suresh CUBE: Yes sir, this was one of the culture weak condition observed in last june month. It revived by the help of other collaborators @Arunan HBSCE sir @KIRAN CUBE mam @Batul Pipewala Kolkata mam @Aastha Ahuja Cube. Iam always thankful for that
.[3:52 PM, 8/17/2021] Arunan HBSCE: How do you expect to get eggs in this bottle? @Aswathy Suresh CUBE Please give your reasoning.
[3:58 PM, 8/17/2021] Arunan HBSCE: Are you not worried that there are no larvae, even after 5 days of transferring 14 flies of which 6 were gravid flies into this bottle?
@Aswathy Suresh CUBEDo you see some problem, again? @Aswathy Suresh CUBE Let’s discuss with every one.
[3:58 PM, 8/17/2021] Aswathy Suresh CUBE: Yes, I still keeping C subcultures bottles. but there is no flies, Larvae, Pupae in those bottles. Bottle where completely empty condition.
[3:59 PM, 8/17/2021] Arunan HBSCE: Good! Looking for your involvement @Shivam CUBE NEMATODE
[4:03 PM, 8/17/2021] Arunan HBSCE: Any reference for how alcohol is formed? @Aditiya Joshi CUBE @Abhijeet Singh @Seethalakshmy CUBE
[4:04 PM, 8/17/2021] Arunan HBSCE: Great! Looking for updates on other model systems including Phyllanthus of your HomeLab @Aditiya Joshi CUBE
[4:05 PM, 8/17/2021] Arunan HBSCE: Why? Any explanation? @Seethalakshmy CUBE
[4:06 PM, 8/17/2021] Arunan HBSCE: So, what exactly is your confusion about? @Seethalakshmy CUBE
[4:07 PM, 8/17/2021] Aswathy Suresh CUBE: Yes I can also see some 3rd instar larvae were curling bottle wall
[4:07 PM, 8/17/2021] Arunan HBSCE: Great idea! We need close up photos of ants too! @Batul Pipewala Kolkata @Seethalakshmy CUBE @Shrudhiga
[4:08 PM, 8/17/2021] Aswathy Suresh CUBE:
[4:10 PM, 8/17/2021] Aswathy Suresh CUBE: Imaginal disc is a sac-like epithelial structure found inside the larva of insects that undergo metamorphosis.
[4:10 PM, 8/17/2021] Aswathy Suresh CUBE: Larva turns into a pupa, almost all the larval tissues degenerate and the imaginal discs turn into the external structures of the head, thorax, limbs and genitalia.
[4:13 PM, 8/17/2021] Arunan HBSCE: Why do you think you missed it earlier?
A nice of Goof Up!
Was it because that was not part of the expectation?
What lessons can we learn and pass on to others from this, as vital lesson in Collaboration during establishing
CUBE HomeLabs! @Aswathy Suresh CUBE @Lydia cUBe Drosphila @Saida Sayyed @Aastha Ahuja Cube @KIRAN CUBE and others
[4:16 PM, 8/17/2021] Arunan HBSCE: What is the lesson in Collaboration from this we learn, as CUBE starts with Collaboratively? @Aswathy Suresh CUBE
[4:18 PM, 8/17/2021] Arunan HBSCE: Let’s highlight that lesson, for others to share their Daily Updates! @Aswathy Suresh CUBE
[4:21 PM, 8/17/2021] Arunan HBSCE: What one sees will be different from what collaborators see!
We have examples, every day!
So, please give photo evidence for your positive as well as negative observations & conclusions, for others to verify! @Aswathy Suresh CUBE
[4:23 PM, 8/17/2021] Arunan HBSCE: @Lydia cUBe Drosphila was asking for and it is good to give evidence @Aswathy Suresh CUBE Who knows, we may see some possibilities!
[4:29 PM, 8/17/2021] Arunan HBSCE: Wow!
@Batul Pipewala Kolkata
What lessons from this for all Cubists?
Surely, @Aswathy Suresh CUBE and others like you and me could come up with this because Aswathy has been consistently keeping her HomeLab active and posting Reports with photos!
Another feather in Aswathy’s cap!
What lessons we learn from this? @Batul Pipewala Kolkata @Seethalakshmy CUBE and others
[4:30 PM, 8/17/2021] Arunan HBSCE: Let’s find out approximately how many? Is it according to the expectations? @Aswathy Suresh CUBE
PHOTO SHARED BY KIRAN AT 4.46PM
[4:46 PM, 8/17/2021] KIRAN CUBE: BREAKING NEWS fruit fly has got emerge out of pupal case on 17th day But found dead. Story of pupae which were found under the lid of dustbin, 5 of the pupae were transferred in plastic container on 1st August 2021.
[5:06 PM, 8/17/2021] KIRAN CUBE: S4E146 of CUBE chatShaala: Difference in the olfaction sensitivity of fruit flies
Kindly click on the above👆🏻 given link for the summary of last discussion.
Invitation to join today’s CUBE chatShaala, 
Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in all CUBE what’s app groups and on metastudio after the session
Here’s the direct link of the CUBE webinar: https://webinar.hbcse.tifr.res.in/b/kir-hye-cn4
Note: we have changed the room link to avoid technical error
Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
KOEL CALL SHARED BYE SEETHALAKSHMY AT 6.06PM
Koel cooing
Location : Pazhuvil
Date:17/8/21
Time: 4:20pm
Data collector: seethalakshmI
VIDEOS SHARED BY BATUL AT 6.11PM
Anterior end of the soil nematode family Rhabditidae showing c)straight stoma(mouth)b)enlarged oesophagus and c)pharyngeal bulb.Observation under 45X of compound microscope.The nematode has been picked up from the raw potato slice subculture B. Cube homelab Kolkata BatulP 17/8/21
Nematodes multiplying on subculture B raw potato slice observed using magnifying glass of 10X and camera zoom4X.17/8/21Cube Homelab Kolkata BatulP
Video showing movement of soil nematodes size approx 2mm under 10X of compound microscope. CUBE Homelab Kolkata BatulP 17/8/21
What about others???
Can you hear the koel cooing in your location ???
@Aditiya Joshi CUBE
@Drishtant Cube Moina, @DP HBCSE sir, @Man Masih Beck RANCHI CUBE, @Shrudhiga, @Aswathy Suresh CUBE, @Sidhy Kerala Drosophila, @Dr. Binumol SN Nattika ma’am, @Batul Pipewala Kolkata ma’am, @Arunan HBSCE sir, @KIRAN CUBE ma’am
And others
None that I can recollect. But are these Koel calls at your place consistent? or random outlier?
No Cuckoo calls heard in my locality at Kokar, Ranchi, Jharkhand.
[6:36 PM, 8/17/2021] Seethalakshmy CUBE: No @DP HBCSE sir, not regularly.
[6:37 PM, 8/17/2021] Seethalakshmy CUBE: Only in my area. 
What about other places in Kerala
Ok, have the rains reduced in Kerala and weathered warmed a little? It could be some false alarm for that Koel. In Mumbai and I guess also in Ranchi, rains are still on…
Cuckoo calls heard a day before but when I was about to record it, it stopped😅
Yes, sir, it rained today as well as sunny.
[6:50 PM, 8/17/2021] Seethalakshmy CUBE: Ok, next time try to record the voice
[6:51 PM, 8/17/2021] Seethalakshmy CUBE: Ok! Is it rain there?
[6:55 PM, 8/17/2021] Seethalakshmy CUBE: Is there any connection between the rain and their calling?
yes. it rains daily in the second half of the day.
Ohk
FILE SHARED BY RAHUL AT 8.46PM
No I dont hear koels calling in my locality
happy to share with the new cubists a collaborative work on earthworm regeneration by ANDC cubists back in 2015-16
Yes please
How do you identify genus and species of earthworm??
516th day of CUBE ChatShaala: 17th August 2021.
22 CUBists from 22 Centres had joined the webinar.
INDIA
ASSAM
Jorhat : Ishita Sonowal
DELHI
DSC, Delhi : Dr P. Chitralekha
HARYANA
Panipat : Aashi
JHARKHAND
Morabadi, Ranchi : Rechel Tirkey
KERALA
Kolenchery : Aparna Saleeph
Thriprayar : Aswathy Suresh
Pazhuvil, Trissur : Seethalakshmi CR
Thanniam, Thrissur : Sidhy PP
MAHARASHTRA
Ulwe, Navi Mumbai : Abhijeet Singh
Breach Candy, South Mumbai : Arunan MC
Powai : Lydia Mathew
Mumbai : Nagarjuna G.
Mulund, Mumbai : Priti Kanade
Dahanu Road, Palghar : Sachin Pradhan
Bandra, Mumbai : Saida Sayyed
Kalyan : Shivam Kumar Sriwas
Panvel : Shraddha Sonavane
TAMIL NADU
PSGR Krishnammal College Coimbatore : Arunima
UTTAR PRADESH
Moradabad : Kiran Yadav
WEST BENGAL
Kolkata : Batul Pipewala
: Ashi
: Meena
Note
Cubist(s) who had joined today but their name isn’t included in the list, kindly replied to this message
