11th August 2021, 9.30pm.to 12th August 2021, 9.30pm
Dr. Chun Mei Chang
have you thought of adding boric acid instead? its a better bactericide and fungicide and won’t change the pH of the media.
Seethalakshmi:
𝐂𝐮𝐫𝐝𝐥𝐢𝐧𝐠 𝐨𝐟 𝐌𝐢𝐥𝐤 . 𝗕𝗶𝗼𝗹𝗼𝗴𝗶𝗰𝗮𝗹 vs 𝗖𝗵𝗲𝗺𝗶𝗰𝗮𝗹 processes.
𝗢𝗯𝘀𝗲𝗿𝘃𝗮𝘁𝗶𝗼𝗻 :
After 6hrs
𝗕𝗲𝗮𝗸𝗲𝗿 𝗔(𝐌𝐢𝐥𝐤+𝐕𝐢𝐧𝐞𝐠𝐚𝐫) : precipitation is observed as well as it starts to semi-solid.
𝗕𝗲𝗮𝗸𝗲𝗿 𝗕 : ( 𝐎𝐧𝐥𝐲 𝐌𝐢𝐥𝐤 ) : A yellow colour thin layer is formed on the top. it is liquidy.
𝗕𝗲𝗮𝗸𝗲𝗿 𝗖 ( 𝐂𝐮𝐫𝐝+𝐌𝐢𝐥𝐤 ) : A yellow colour layer is formed. Semi solid.
Location : Pazhuvil .
Date of setup:11/8/21
Time:5:20 Pm
Data collector : Seethalakshmi .C.R. CUBE Nattika .
[8/12, 12:48 AM] Seethalakshmy CUBE: Great ! @Lakshmy PJ CUBE
So days passing,more seeds starts to germinate right?
Why some seeds germinate late?
These all seeds are from a single plant?
[8/12, 12:50 AM] Seethalakshmy CUBE: How many days after sowing did you get this result?@Lakshmy PJ CUBE
[8/12, 12:57 AM] Seethalakshmy CUBE: Yes @KIRAN CUBE ma’am you are right.
Can you Tell us "𝗦𝘂𝗺𝗺𝗮𝗿𝘆 𝗼𝗳 𝗖𝗮𝗿𝗱𝗮𝗺𝗶𝗻𝗲 𝗶𝗻 𝘆𝗼𝘂𝗿 𝗖𝗨𝗕𝗘 𝗛𝗼𝗺𝗲 𝗟𝗮𝗯 "
That will help to me . To overcome my problems.
[8/12, 9:16 AM] Arunan HBSCE: What is your interpretation? @Seethalakshmy CUBE
[8/12, 10:06 AM] Batul Pipewala Kolkata:
will try[8/12, 10:09 AM] Arunan HBSCE: Are you convinced? @Batul Pipewala Kolkata can’t see any fungus in this. @Batul Pipewala Kolkata
[8/12, 10:13 AM] Batul Pipewala Kolkata: I have added two drops of vinegar but last time I added vinegar 5ml the nematodes did not survive so if they do not survive this time I will look for an alternative as boric acid.
[8/12, 10:13 AM] Arunan HBSCE: Please share with others what is the approach to identify based on eggs?
Color of eggs? Shape of eggs? Single eggs vs joined together?
Which references are authentic to base on?
Please share with all?
Is Vyom Gupta actively involved? Please encourage your students to post to the CUBE groups. @Hina Mudgal Kanpur CUBE
[8/12, 10:15 AM] Arunan HBSCE: Any idea which are the host plants of this butterfly?
How will we find it? @Shrudhiga
Any clear references?
[8/12, 10:17 AM] Arunan HBSCE: How about designing better traps for Fruitflies?
Please give your idea, based on your experience as well as from the experience of other cubists? @Janhvi Deshmukh
Batul:
Original nematode culture started on 29/7/21 after 14days has begun to show more dead nematodes or immobile nematodes and is smelling awful so I am discarding it.The nematodes have already been transferred to a fresh raw potato slice on 6/8/21 and are doing well
Please clarify this @Seethalakshmy CUBE
Batul:
Dead and immobile nematodes in the original potato culture of 29/7/21Cube homelab Kolkata BatulP 12/8/21
[8/12, 10:21 AM] Arunan HBSCE: How do compare the smelling capacity of recently trapped with flies from 1 year old cultures? @Seethalakshmy CUBE @Aswathy Suresh CUBE @Man Masih Beck RANCHI CUBE
What will be the logic & Design of the experiment?
[8/12, 10:25 AM] Arunan HBSCE: Please post videos of all bottles of SLBK A and SLBK C along with SLBK B & SLBK E.
Please help others to see what is in all these bottles. @Aswathy Suresh CUBE
Let’s also know how to take good videos.
[8/12, 10:26 AM] Arunan HBSCE: What is the source of this nematode? Please give the Context.
What is your immediate plan? @Batul Pipewala Kolkata
[8/12, 10:28 AM] Arunan HBSCE: Please post photos to convince others of what you were able to see.
It may so happen, others may see more flies or larvae or pupae.
Please post to convince others. @Aswathy Suresh CUBE @Yash Sheregare CUBE
[8/12, 10:29 AM] Arunan HBSCE: What is your immediate plan? @Aswathy Suresh CUBE
[8/12, 10:30 AM] Arunan HBSCE: @Aswathy Suresh CUBE please follow up to convince others including @Aastha Ahuja Cube
[8/12, 10:31 AM] Batul Pipewala Kolkata: The source of nematodes in this slide is from rava sugar medium.
[8/12, 10:31 AM] Arunan HBSCE: What do you really mean by Olfaction Experiment? @Priti Kanade @Aswathy Suresh CUBE @Man Masih Beck RANCHI CUBE
Please describe.
[8/12, 10:33 AM] Arunan HBSCE: Please post more pictures of SLBK C for others to get convinced @Aswathy Suresh CUBE
@Aastha Ahuja Cube What is your comment?
Shraddha:
Please identify. Black hairy Caterpillar observed on Phyllanthus urinaria plant. Date-12/8/21 location-Panvel. data collected by-Shraddha
[8/12, 10:35 AM] Batul Pipewala Kolkata: Why dont you keep the Caterpillar alongwith some phyllanthus leaves to see it grow into an adult ?
[8/12, 10:36 AM] Arunan HBSCE: Bubbles? What bubbles? Please post photos. @Aswathy Suresh CUBE
Let’s analyse collaboratively and understand what was going on in this bottle. @Aastha Ahuja Cube @Aswathy Suresh CUBE @Yash Sheregare CUBE
[8/12, 10:39 AM] Arunan HBSCE: Which SLBKC bottle was the last good culture? @Aswathy Suresh CUBE
Please post photos from your archive.
What do you say? @Aastha Ahuja Cube @Yash Sheregare CUBE @Man Masih Beck RANCHI CUBE
[8/12, 10:41 AM] Batul Pipewala Kolkata: Microbiome consists of microbes like bacteria and others present inside the organism and in their immediate surroundings.
the wild, C. elegans harbors a characteristic gut microbiome community that is recruited from its surrounding environment (Dirksen et al. … This set is composed of 12 bacteria from 9 different families that represent the core microbiome of C. elegans based on analyses and empirical studies of intestinal colonization.
https://www.g3journal.org/content/10/9/3025#:~:text=In%20the%20wild%2C%20C.,surrounding%20environment%20(Dirksen%20et%20al.&text=This%20set%20is%20composed%20of,empirical%20studies%20of%20intestinal%20colonization.
[8/12, 10:45 AM] Arunan HBSCE: This requires analysis @Aastha Ahuja Cube @Aswathy Suresh CUBE @Man Masih Beck RANCHI CUBE @Yash Sheregare CUBE
Please post photos of this bottles from your archives from 02 .07.2021 @Aswathy Suresh CUBE
Let’s analyse it collaboratively, because how can 30 flies transferred into a medium bottle have no good culture in the form of larvae & pupae? Interesting to understand what was gone wrong? A great opportunity!
@Aswathy Suresh CUBE @Aastha Ahuja Cube @Yash Sheregare CUBE
[8/12, 10:46 AM] Arunan HBSCE: Wah, wah! In how many days? @Lakshmy PJ CUBE
[8/12, 10:49 AM] Arunan HBSCE: Which mosquitoes lay single, scattered eggs? Which mosquitoes do lay eggs in groups attached to each other? @Hina Mudgal Kanpur CUBE @Rahil and others.
Let’s continue discussion to understand that identification can not be difficult. @Hina Mudgal Kanpur CUBE
[8/12, 10:57 AM] +91 22237: Butterflies of Singapore: Life History of the Short Banded Sailor).
This is the reference for its host plant… Its names are not familiar for me
VIDEO SHARED BY SHRUDIGA AT 11.05am
Date-12/8/2021 Time-11.00AM Location - Cube home lab Thathamangalam Data collector- Shrudhiga
VIDEO SHARED BY MAN AT 11.07AM
Astonished by its color dissimilarity in the bottom 
and the upper region of the plant 
…And this color varies from dark green to Light reddish-brown…Why does this vary from plant to plant and from region to region specifically the upper and bottom portion…Is my plant not healthy inside the bag
Date-12/8/2021 Time-11.00AM Location - Cube home lab Thathamangalam Data collector- Shrudhiga
Video shared by Man at 11.07am
ReMan’s Lab, Ranchi 12-08-2021 Alert! Alert! Immediate Objective: To save the Brown Hydra at ReMan’s Lab Video: ~1 cm long(head to foot) Brown Hydra at ReMan’s Lab first observed on 8th Aug, 2021 by @Rechel Turkey Cube Ranchi Moina How you can help: As we are left with no moina, suggestions for alternative feed or design to maintain Hydra is needed urgently @Seethalakshmy CUBE @Aswathy Suresh CUBE @Drishtant Cube Moina @Rechel Turkey Cube Ranchi Moina @Abhijith Vinod Cube Kolenchary Drosophila and others…
Aswathy: PHOTO
new subcultures
SLBK A 22 ; SLBK B21 ; SLBK E17
Model system: Fruit Flies
Transfer date : 12.08.2021
Media Date : 11.08.2021
Single Line Cultures in Bottukadavu Thrissur Kerala A, B, E Line Have Today.
•SLBK A22 - Transferred 1 Fly
•SLBK B21 - Transferred 12 Flies
•SLBK E17 - Transferred 14 Flies
Collaborators : @Arunan HBSCE sir @Yash Sheregare CUBE @Isha Pawle @Aastha Ahuja Cube @Janhvi Deshmukh @Rechel Turkey Cube Ranchi Moina
VIDEO SHARED BY ASWATHY AT 11.30AM
SLBK A22 - A Line Culture
Transfer Date : 12.08.2021
Model System : Fruit Flies
Media : TRSV ; 11/08/2021
Transfer Flies : 1 Fly (Look Like Gravid One)
Expectations :
13.08.2021 : 1st Instar Larvae
14.08.2021 : 2nd Instar Larvae
15.08.2021 : 3rd Instar Larvae
16.08.2021 : Pupae (4th Day)
20.08.2021 : Next generation flies (8th Day).
Observation : 1st Day
Media perfectly solidified condition. Fly is happily feeding media, moving.
Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
[11:44 AM, 8/12/2021] Aswathy Suresh CUBE: SLBK B21 - B Line Culture
Transfer Date : 12.08.2021
Model System : Fruit Flies
Media : TRSV ; 11/08/2021
Transfer Flies : 12 Flies
Gravid Flies : 5 Flies
Male Flies : 7 Flies
Expectation :
13.08.2021 : ~20 - 1st Instar Larvae
14.08.2021 : ~30 - 1st & 2nd Instar Larvae
15.08.2021 : ~40 - 1st, 2nd & 3rd Instar Larvae
16.08.2021 : ~20 - Pupae + ~20 Larvae (Different stage)
20.08.2021 : ~20 new flies + ~30 - Pupae + ~30 Larvae (Different stage)
Observation : 1st Day
Media is Solidified.Flies are happily moving around. Expecting some eggs.
Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur Kerala.
[11:49 AM, 8/12/2021] Aswathy Suresh CUBE: SLBK E17 - E Line Culture
Transfer Date : 12.08.2021
Model System : Fruit Flies
Media : TRSV ; 11/08/2021
Transfer Flies : 14 Flies
Gravid Flies : 6 Flies
Male Flies : 8 Flies
Expectation :
13.08.2021 : ~30 - 1st Instar Larvae
14.08.2021 : ~40 - 1st & 2nd Instar Larvae
15.08.2021 : ~50 - 1st, 2nd & 3rd Instar Larvae
16.08.2021 : ~25 - Pupae + ~30 Larvae (Different stage)
20.08.2021 : ~25 new flies + ~30 - Pupae + ~60 Larvae (Different stage)
Observation : 1st Day
Media is Solidified.Water droplets present on bottle wall.Flies are happily moving around. Expecting some eggs.
Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur Kerala.
[11:51 AM, 8/12/2021] Arunan HBSCE: What is the amount of vinegar or acetic acid that prevents microbial growth, according to you? @Batul Pipewala Kolkata
What does references tell on this?
Let’s understand the scientific basis at every step, even when we want to discard the design, in a convincing manner. @Batul Pipewala Kolkata
Let’s show what is different in the practice of Science, than just trial and error resorted to, by others@Batul Pipewala Kolkata
[11:53 AM, 8/12/2021] Arunan HBSCE: I can see some fly larvae there! @Batul Pipewala Kolkata
[12:24 PM, 8/12/2021] Arunan HBSCE: Why do you think these nematodes are dead? They may be immobile! Look, they are not disintegrating! They look very intact!
Please give us reasons @Batul Pipewala Kolkata
[12:26 PM, 8/12/2021] Arunan HBSCE: Always give the context for others to followoe the story.
Are you sure this was Rava Sugar medium or Rava Sugar Vinegar medium? @Batul Pipewala Kolkata
[12:28 PM, 8/12/2021] Arunan HBSCE: Are these caterpillars feeding on Phyllanthus or they just happened to be there by chance? @Shraddha Sonavane CUBE
What are considered to be pests of Phyllanthus plants? Any reference? @Shraddha Sonavane CUBE
[12:38 PM, 8/12/2021] Arunan HBSCE: This should be announced as BREAKING NEWS! @Lakshmy PJ CUBE
[12:41 PM, 8/12/2021] Arunan HBSCE: Why study Lifecycle of this dangerous animal?
@Hina Mudgal Kanpur CUBE
Is it not going to be a public health issue? @Hina Mudgal Kanpur CUBE
[12:43 PM, 8/12/2021] Arunan HBSCE: What do you mean by ’ true curd’? @Seethalakshmy CUBE
Any reference? @Seethalakshmy CUBE @Jai Kishan CUBE and others
[12:46 PM, 8/12/2021] Arunan HBSCE: Won’t these be two different species?
On the other hand , if we want to prove that one medium ( rava- sugar) is better than the other medium (potato), what will be the DESIGN of the experiment? @Batul Pipewala Kolkata
[12:46 PM, 8/12/2021] Drishtant Cube Moina: If not Moina, then you should feed other crustaceans such as Copepods, Ostracods that are common in freshwater habitats. I think you should look around for them in the same waterbody from where you came across the Brown Hydra.
What do others say?
[12:50 PM, 8/12/2021] Arunan HBSCE: Please list the names @Shrudhiga along with common names.
Let’s make ourselves familiar through this collaborative exercise. @Shrudhiga
[12:52 PM, 8/12/2021] Arunan HBSCE: What is your objective? What is the context? @Shrudhiga
[12:52 PM, 8/12/2021] Man Masih Beck RANCHI CUBE: 
yes… copepods are present at my lab
[12:53 PM, 8/12/2021] Arunan HBSCE: Which plant is this according to you? @Shrudhiga
[12:53 PM, 8/12/2021] Arunan HBSCE: Any resemblance to any other plants? @Shrudhiga
[12:55 PM, 8/12/2021] Arunan HBSCE: Excellent! @Man Masih Beck RANCHI CUBE Where did you get the Hydra from? @Man Masih Beck RANCHI CUBE @Rechel Turkey Cube Ranchi Moina
[12:58 PM, 8/12/2021] Arunan HBSCE: Any special care did you take this time while making and transferring the medium, knowing that last time all 4 bottles did not function equally well? @Aswathy Suresh CUBE
Hope, you had a better design this time. Please describe @Aswathy Suresh CUBE @Yash Sheregare CUBE @Lydia cUBe Drosphila @Man Masih Beck RANCHI CUBE @Saida Sayyed
[1:00 PM, 8/12/2021] Arunan HBSCE: Please take photos with good even background @Aswathy Suresh CUBE
[1:02 PM, 8/12/2021] Arunan HBSCE: Is this not a BREAKING NEWS , that you are starting a culture from a single fly, without knowing whether it is male, female. If female, did it get an opportunity to mate and fertilize its eggs? @Aswathy Suresh CUBE @Seethalakshmy CUBE @Yash Sheregare CUBE @KIRAN CUBE and others
[1:03 PM, 8/12/2021] Arunan HBSCE: What is your expectation? @Aswathy Suresh CUBE
[1:05 PM, 8/12/2021] Arunan HBSCE: Let’s hear, from where these Hydra were harvested and when.
What is the story of these Hydra, so far?
[1:05 PM, 8/12/2021] Arunan HBSCE: Please describe @Man Masih Beck RANCHI CUBE @Rechel Turkey Cube Ranchi Moina
(Old post) ReMan’s Lab Ranchi
*Accidental Discovery by @Rechel Turkey Cube Ranchi Moina *
Aug 8, 2021
Sample collection- 24th July, 2021
Location- Pond from Birsa Agricultural University(BAU)'s campus
Using the plankton net lent from @Dr. G C Baskey Cube Ranchi, Dept. of Zoology, DSPMU Ranchi, Rechel and I(Man Masih) collected water samples from the BAU campus. The objective was to discover native Daphnia or Moina species from Ranchi. Although we found many Cyclops and few other crustaceans in the sample, we couldn’t find any moina or daphnia. Since it seemed that the water samples were insignificant for us, Rechel suggested me to discard them which I didn’t due to my laziness. Today, Rechel visited ReMan’s Lab again and after observing the samples she found the Brown Hydra.
Note- For 14 days,(24th July to 7th Aug) the samples remained unnoticed.
Further Plans
We want to develop a design to study Regeneration in Brown Hydra
@Arunan HBSCE @Shalini CUBE Rachi @Dr. G C Baskey Cube Ranchi @Abhijith Vinod Cube Kolenchary Drosophila @Binita Cube
VIDEO SHARED BY SEETHALAKSHMY AT 1.12PM
[1:12 PM, 8/12/2021] Seethalakshmy CUBE: 𝗨𝗽𝗱𝗮𝘁𝗲 𝗼𝗻 𝗔𝗡𝗧 𝗠𝗼𝗱𝗲𝗹 𝗦𝘆𝘀𝘁𝗲𝗺 .
𝗜𝗺𝗺𝗲𝗱𝗶𝗮𝘁𝗶𝘃𝗲 𝗼𝗯𝗷𝗲𝗰𝘁𝗶𝘃𝗲 : 𝗧𝗼 𝗰𝘂𝗹𝘁𝘂𝗿𝗲 𝘁𝗵𝗲 𝗔𝗻𝘁𝘀.
Date of setup :24/5/21
Date of photo:12/8/21
Time:12:51 pm
Location: Pazhuvil ,CUBE Home Lab
Data collector: Seethalakshmi.C.R.
[2:06 PM, 8/12/2021] Ankit Yadav Xms Work Shop Cube Patkar College: How do you culture ants??
[2:19 PM, 8/12/2021] Aswathy Suresh CUBE: Immediate plan : Make new media and transfer remained one fly. That one fly look like gravid one. So, Iam expecting some new progeny’s will get from this gravid fly.
It was SLBK C19 ; 13.06.2021 made subculture bottles had good number of ~40 more flies, ~25 more larvae, ~60 more Pupae shell observed. That was the last Good culture bottle of C - line.
VIDEO SHARED BY ASWATHY AT 2.32PM
[2:32 PM, 8/12/2021] Aswathy Suresh CUBE: SLBK C19 - 13.06.2021
(subculture of C species)
Transfer flies : ~30
Gravid flies : ~15
Male flies : ~15
15 Days old
Conditions
•~+40 adult flies.
•~25 Pupate.
•~30 Dead flies.
•~15 - 3mm sized Larvae.
•Microworms still observed inside culture bottle.
Update by : Aswathy Suresh CUBE SN Nattika.
[2:34 PM, 8/12/2021] Aswathy Suresh CUBE: This was the last good culture of C - Line C19 
[2:51 PM, 8/12/2021] Seethalakshmy CUBE: First we set the formicarium.
This is our Formicarium
Formicarium is used to culture the ants.
Date of setup : 24/5/21
Location : pazhuvil
Time :10:00am
materials required for formicarium
*plastic /glass jar
*Soil and stones
*two bottle caps
(1.Sugar
2. Water)
How do make
*we need plastic /glass jar (here i am using 34cm length jar)
*Put some soil and stones to repeat this process
Now the jar filled
*take two lids of bottles
Take sugar and water and kept on the jar,
Wait for ants.
After these setup i got some ants.
[2:57 PM, 8/12/2021] Batul Pipewala Kolkata: What if you collect some pond water it must have some copepods and water fleas you can try feeding the brown hydra with that
[2:59 PM, 8/12/2021] Batul Pipewala Kolkata: How do you know the fly is gravid by looking at it? Do you use your mobile zoom?
[3:03 PM, 8/12/2021] Batul Pipewala Kolkata: Nematodes survived in rava sugar medium without vinegar@@Arunan HBSCE sir
[3:36 PM, 8/12/2021] Arunan HBSCE: Fantastic! Another Goof-up for celebration!
"The objective was to discover native Daphnia or Moina species from Ranchi.
“Although we found many Cyclops and few other crustaceans in the sample, we couldn’t find any moina or daphnia. Since it seemed that the water samples were insignificant for us, Rechel suggested me to discard them which I didn’t due to my laziness.
Today, Rechel visited ReMan’s Lab again and after observing the samples she found the Brown Hydra.”
“Note- For 14 days,(24th July to 7th Aug) the samples remained unnoticed.”
@Man Masih Beck RANCHI CUBE
Please write a separate account, clearly to celebrate your Goof-ups! @Man Masih Beck RANCHI CUBE &@Rechel Turkey Cube Ranchi Moina
[3:42 PM, 8/12/2021] Arunan HBSCE: @Man Masih Beck RANCHI CUBE writes, “Further Plans
We want to develop a design to study Regeneration in Brown Hydra”.
@Man Masih Beck RANCHI CUBE Is not culturing the Hydra your immediate Objective?
Don’t we have a question how the non green hydra survived so long?
Any explanation?
Please post lavish photos of your set up, to inspire others. @Man Masih Beck RANCHI CUBE @Rechel Turkey Cube Ranchi Moina
How about, posting photo of the habitat from where you collected the samples?
Why not tell us how you collected samples.
Exciting Goof-up and chance findings of a treasure! @Man Masih Beck RANCHI CUBE @Rechel Turkey Cube Ranchi Moina @Dr. G C Baskey Cube Ranchi
[3:45 PM, 8/12/2021] Arunan HBSCE: Please post magnified images with a reference ruler for size and to start identifying from today onward. @Seethalakshmy CUBE
Any clear idea which species of worker ants are these?
Please guess and state which ants these are. @Seethalakshmy CUBE @Shrudhiga
[3:48 PM, 8/12/2021] Arunan HBSCE: Don’t you think this foaming is the result of microbial growth?
Do you see this in all bottles made on the same day?
Which bottles have this foaming?
Please post photos of all 4 bottles of made on 19July to see all have this foaming. @Aswathy Suresh CUBE
[3:49 PM, 8/12/2021] Batul Pipewala Kolkata: The rava sugar medium with 5ml vinegar did not help nematode survive,without vinegar growth is v good but the medium developed fungus in 6days.So this time I am using rava sugar with two drops of vinegar to see if it works
[3:51 PM, 8/12/2021] Arunan HBSCE: 30 dead flies after how many days, in this bottle?
And when did you transfer flies from this to the next bottles?
Please give a summary.
[3:52 PM, 8/12/2021] Batul Pipewala Kolkata: Sir many of them were disintegrating and intact ones were immobile they will die too without feeding. Duaer larvae form at a certain stage in which larva undergoes changes
[3:53 PM, 8/12/2021] Arunan HBSCE: Don’t you think waiting for 40 days for transferring to fresh medium is too risky? @Aswathy Suresh CUBE
What will be the ideal time before which we should transfer flies, to fresh culture bottles? @Aswathy Suresh CUBE @Aastha Ahuja Cube @Yash Sheregare CUBE @Sidhy Kerala Drosophila
[3:55 PM, 8/12/2021] Batul Pipewala Kolkata: 15ml vinegar is being used for 100ml medium to prevent fungal growth in TRSV so 1ml or 2 to 3 drops vinegar is good for 10ml medium which I have made for my petri plate.
[3:57 PM, 8/12/2021] Arunan HBSCE: When did you transfer @SLBK C 19 flies to SLBKC 20 bottles? @Aswathy Suresh CUBE
Why did you wait for so long? Any special reason or we don’t have a definite DESIGN yet to MAINTAIN STOCK CULTURE of Fruitflies?
@Aswathy Suresh CUBE @Yash Sheregare CUBE @Man Masih Beck RANCHI CUBE @Lydia cUBe Drosphila @KIRAN CUBE
[3:59 PM, 8/12/2021] Arunan HBSCE: Is that not dangerous, by providing opportunities for microbe to have field day? @Batul Pipewala Kolkata
[4:01 PM, 8/12/2021] Arunan HBSCE: What exactly was your design of experiment, to throw out that hypothesis? @Batul Pipewala Kolkata
Did you have sufficient Replicates and Controls before ruling out the possibility?@Batul Pipewala Kolkata
[4:03 PM, 8/12/2021] Arunan HBSCE: Evidence,please! The photo does not substantiate your claim! Rather, all worms and eggs are intact!
Please provide evidence.
"Let’s base all our decisions on OBJECTIVE EVIDENCE*. @Batul Pipewala Kolkata
[4:07 PM, 8/12/2021] Arunan HBSCE: How many replicates? @Batul Pipewala Kolkata What about Controls?
Please show us photo of the set up with which we are ruling out the possibility that nematodes could grow in TRSV medium!
And @Aswathy Suresh CUBE has proved it by chance in her TRSV medium!
[4:09 PM, 8/12/2021] Arunan HBSCE: Please write down all details to falsify the Hypothesis that TRSV medium supports growth of Nematodes.
Janhvi: PhOTO
[4:26 PM, 8/12/2021] Janhvi Deshmukh: I see some fruitfly feeding on bittergourd
[4:26 PM, 8/12/2021] Janhvi Deshmukh: Janhvi deshmukh homelab bajaj collage of science wardha
[4:35 PM, 8/12/2021] Shraddha Sonavane CUBE:
[5:07 PM, 8/12/2021] KIRAN CUBE: INVITATION TO JOIN TODAY’S CUBE CHATSHAALA
Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in CUBE what’s app groups after the session
Webinar Link : https://webinar.hbcse.tifr.res.in/b/kir-vgc-6jf
Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
[5:46 PM, 8/12/2021] KIRAN CUBE: Quickly join, today we have kalpana fellows in the CUBE chatShaala.
Currently introducing them to Moina
@Aswathy Suresh CUBE @Lakshmy PJ CUBE @Aastha Ahuja Cube @Ishita Assam and other Cubists
[5:58 PM, 8/12/2021] Sunita Chahar CUBE: Kiran
Our students are not able to join
[5:58 PM, 8/12/2021] Sunita Chahar CUBE: Server error today
[6:02 PM, 8/12/2021] Sunita Chahar CUBE: I too tried,its showing server error
[6:08 PM, 8/12/2021] Batul Pipewala Kolkata: Added Nalini Singh of Cube Ratnam college please share your work on Nematodes here@@Nalini Singh
[6:09 PM, 8/12/2021] Sunita Chahar CUBE: Give some direct link Kiran, students are struggling to join
[6:09 PM, 8/12/2021] KIRAN CUBE: Direct link
https://webinar.hbcse.tifr.res.in/b/kir-vgc-6jf
[6:17 PM, 8/12/2021] Batul Pipewala Kolkata: Added Shahin of Cube Ratnam college please share your work on Nematodes here giving your location and date@@Shahin🍁
[6:18 PM, 8/12/2021] +91 19166: Thank you for joining us maam there are two more members akansha yadav and poonam yadav
[6:18 PM, 8/12/2021] Janhvi Deshmukh: Welcome Shahin 
please share your experiment with us 
[6:18 PM, 8/12/2021] Batul Pipewala Kolkata: Their WhatsApp numbers please
[6:19 PM, 8/12/2021] +919166: +91 03954 akansha yadav
[6:20 PM, 8/12/2021] KIRAN CUBE: Attention!
Owing to unexpected technical issues regarding the regular Big Blue Button Platform where the CUBE Chatshaala is held everyday, we are shifting (temporarily, for today) to
Jitsi.
In case of any issues, please let us know in the group or on personal.
[6:25 PM, 8/12/2021] +91 19166: +912194 poonam yadav
[6:29 PM, 8/12/2021] KIRAN CUBE: Attention!
Owing to unexpected technical issues regarding the regular Big Blue Button Platform where the CUBE Chatshaala is held everyday, we are shifting (temporarily, for today) to
Jitsi.
In case of any issues, please let us know in the group or on personal.
[6:34 PM, 8/12/2021] Lakshmy PJ CUBE: I collected cardamine plants from yellow seed pods.
In one single seed pod there are 19 seeds.
The seeds were yellow coloured.
Its just like sigle line culture like @Aswathy Suresh CUBE. I am trying to make pure line of cardamine plants.
[6:36 PM, 8/12/2021] Lakshmy PJ CUBE: We can go with the method that we followed in Phyllanthus identification.
By looking on to their leaves, stem, leaves, flowers and fruits. @KIRAN CUBE
[6:38 PM, 8/12/2021] Lakshmy PJ CUBE: Within 10 days. @Arunan HBSCE
[6:43 PM, 8/12/2021] Lakshmy PJ CUBE: Shraddha could you send much more clear picture.
Is it clear to you @Lakshmy PJ CUBE
Sunita: PHOTO OF PHYLLANTHUS
[7:17 PM, 8/12/2021] Sunita Chahar CUBE: From LED to natural light
[7:17 PM, 8/12/2021] Sunita Chahar CUBE: 9th August to 12th August
[7:17 PM, 8/12/2021] Sunita Chahar CUBE: 4 days over
[7:18 PM, 8/12/2021] Sunita Chahar CUBE: Not revived
Photo by Sunita Chahar
Hemant:
[7:21 PM, 8/12/2021] +910727: Why this snails seeing increased in maharashtra kolhapur district farm area
[7:21 PM, 8/12/2021] Sunita Chahar CUBE: Plants in natural light
[7:21 PM, 8/12/2021] +910727: Specifically after last rain
Transfer 1 Fly
@Aswathy Suresh CUBE Let’s expect some visible larvae in 2 days!
SLPbA2 on 7/08/2021
wow what is your next plan 
wow !what they do in hibiscus 
…so it’s a phylluntus amarus … Janhvi deshmukh homelab bajaj collage of science wardha maharshtra
Here’s the direct link of the CUBE chatShaala webinar: 
No of Unhatched Pupae : ~40
This video is so amazing! We can see the white stripes on the back of ants.
Date:- 19/08/2021, Time: 2.15pm, Location:- Ulwe, Navi Mumbai, Maharashtra.
oxalis
@Shahin:maple_leaf:
on Cardamine plant:seedling: in 2nd right side patch of Cardamine forest in front of HBCSE main building.
was emerged.
for Psyche butterflies.
with these characteristics that you think are morphological features.
legs quite visible?
1)Involved in Discussion
Water:cup_with_straw:( 150 ml)
(~50 gm)
Rava:rice:(20 gm)
(15 gm)
Remove the bottle and we can see flies getting immobilise.
?
species, right 
_agariplaadil..JPG)
and Ask Why tomorrow!
In yesterday’s chatshala, we started discussing with the microscopic picture which was shared by 
, like from tap water using nutrient rich coconut water. 
