Archives of CUBE Context 2 Curriculum what's app group discussion

[KiranKalakotiR (Kiran Kalakoti)]

17th August 2021, 9.30pm to 18th August 2021, 9.30pm
@Aswathy Suresh CUBE @Saida Sayyed @Abhijeet Singh @aashi.

Why finding mean is important? What’s the context of discussing mean in case of olfactometer?
@Shraddha Sonavane CUBE we all remember your wonderful work that you have done on Phyllanthus recently.

Any follow up or update on your work?.
What does this whiteboard tells us?
What’s the Chai and Why? Session
[11:32 PM, 8/17/2021] Aswathy Suresh CUBE: @Isha Pawle please give your single line cultures of fruit flies in Worli - Mumbai

•How many Single Line Culture you made? When?

•Can you please tell the names of single line culture that you made.

•How did you trapped fruit flies?

Please give your current Home Lab Updates
[11:36 PM, 8/17/2021] Aswathy Suresh CUBE: @Yash Sheregare CUBE please give the updates from your home lab fruit flies culture in Prabhadevi - Mumbai.

•How many subculture you made newly?

•What is the current condition of your single line cultures today?
[11:40 PM, 8/17/2021] Aswathy Suresh CUBE: @Janhvi Deshmukh Do you keep any new setups for to trap fruit flies in Wardha - Mumbai?

•What is your further plan?

•Do you still facing any issues while trapping fruit flies?

Please give update from your Home Lab.
[11:46 PM, 8/17/2021] Aswathy Suresh CUBE: Understanding the confusion in understanding how to calculate mean (we should highlight some of such kind of instance in chai and why? session that we take for granted)…Collaboration, Communication, curiousity, context to curriculum and consistency.
[11:51 PM, 8/17/2021] Aswathy Suresh CUBE: Chai & Why

TIFR’s unique outreach initiative since 2009 has been the “Chai and Why?” series, a forum to enable informal discussions of interesting scientific issues outside a traditional academic setting in a Science Café format.
http://univ.tifr.res.in/gs2021/Gallery/Images/022-Canteen.JPG
[12:28 AM, 8/18/2021] Seethalakshmy CUBE: I think the larvae not is visible at everytime. I didn’t much have idea about it, how can collect it’s larvae in the formicarium???
Please give some suggestions!
@Batul Pipewala Kolkata ma’am, @Man Masih Beck RANCHI CUBE, @Arunan HBSCE sir, @Shrudhiga, @KIRAN CUBE ma’am, @Vishnupriya CUBE, @Aditiya Joshi CUBE, @Sunita Chahar CUBE ma’am and others…
[12:46 AM, 8/18/2021] Seethalakshmy CUBE: The gelatin dessert “Jello” gels due to the presence of a protein. When Jello is heated, its structure is altered so that it is no longer a gel but a liquid. If the denaturing conditions reversed by cooling in the refrigerator, the protein reforms into its original gel structure.
[12:47 AM, 8/18/2021] Seethalakshmy CUBE: Yes ma’am. This is a good idea! But how can collect their larvae in to the formicarium???
As well as how can feed them?
[12:51 AM, 8/18/2021] Seethalakshmy CUBE: Yes sir, that’s iam trying to say some of them are carrying inside the formicarium, as well as they carriying to out side the formicarium.
[12:51 AM, 8/18/2021] Seethalakshmy CUBE: Yes sir!
[12:56 AM, 8/18/2021] Seethalakshmy CUBE: Yes sir! That’s i want to find. Which species of ant these???
I need others help for identifying the Ant species.
Cubists can help me to overcome this problem.
This is a major problem that i faced my Model system.
Here iam stuck!
[12:58 AM, 8/18/2021] Seethalakshmy CUBE: Yes sir! Do all ants have same type of life cycle??
Please help me to find out Crazy Ant’s life cycle.
@Batul Pipewala Kolkata ma’am, @KIRAN CUBE ma’am, @Man Masih Beck RANCHI CUBE, @Arunan HBSCE sir, @Aswathy Suresh CUBE, @Sunita Chahar CUBE ma’am, @Aditiya Joshi CUBE,@Lakshmy PJ CUBE…
And others
[1:01 AM, 8/18/2021] Seethalakshmy CUBE: Yes sir,
What is imaginal disk??
Can you explain it???
@Aswathy Suresh CUBE, @Saida Sayyed, @Abhijeet Singh, @KIRAN CUBE ma’am and others
[1:04 AM, 8/18/2021] Seethalakshmy CUBE: My confusion is how can protien get refold??
It have 2 category like reversible and irreversible reaction??
PHOTO AND VIDEO OF POTATO SLICE SHARED BY BATUL AT 10.36PM
Soil Nematodes subcultureB after 12days started on 6thAug with raw potato slice with 3to 4 drops of milk and nematodes from original culture.18/8/21Cube homelab Kolkata BatulP
Close up video of the nematodes size approx 1.5mm to 2mm using magnifying glass and mobile camera .The culture is 12days old on raw potato slice.Cube homelab Kolkata BatulP 18/8/21

After 24 hrs of fermentation. Left side bottle is open bottle. Right side bottle is closed bottle. Marked blue(left bottle/open) we can see a whitish layer formed. Marked Red(right bottle/closed) a thin whitish layer formed. Date:17/8/2021. Aditya Joshi. Kala Talav, Kalyan west

Small bubbles are seen in open bottle. Smells different from original coconut water. Somewhat smells like near bakery or smelly bread. 17/8/2021. Aditya Joshi. Kala Talav, Kalyan west. 24 hrs of fermentation
[11:07 AM, 8/18/2021] Seethalakshmy CUBE: So what is your interpretation?
[11:07 AM, 8/18/2021] Seethalakshmy CUBE: @Aditiya Joshi CUBE

48 hrs of fermentation… A thicker whitish layer in open bottle. 18/8/2021. Aditya Joshi. Kala Talav, Kalyan west.

Whitish layer in closed bottle after 48 hrs of fermentation. Aditya Joshi. Kala Talav Kalyan west. 18/8/2021
Only the open bottle shows bubbles?
Why the bubbles forms?

Bottle was swirled or shaken and then was tilted. The bus was dipped and then it was spread on potato slices. I used both hands. But to take picture, bottle was placed on ground n tilted.

18/8/2021. The fermented coconut water was spread on the boiled potato slices. Method: boiled potato slices were taken in a container with lid. A ear bud was used to take the fermented coconut water from the bottles. One bud for open bottle and one for closed bottle. The bud was dipped/soaked in the fermented coconut water. This bud was then spreaded on potato in S shape. Marked red are inoculated slices with open bottle coconut water. Marked blue are slices with closed bottle coconut water. Marked green are the non inoculated potato slices. Expectation: No colonies seen on non inoculated potato slices. Colonies should appear on inoculated slices. Date: 18/8/2021. Aditya Joshi. Kala Talav, Kalyan west.
[12:07 PM, 8/18/2021] Aditiya Joshi CUBE: I think these are the signs of fermentation
[12:08 PM, 8/18/2021] Aditiya Joshi CUBE: No. Some small bubbles were also in closed bottle.
Maybe these are of carbon dioxide released during fermentatiom
[12:08 PM, 8/18/2021] Aditiya Joshi CUBE: Fermentation*
Slices are kept in closed plastic box
See the point is that @Seethalakshmy CUBE that we are constantly trying explain the stuff that we are doing, in very basic and simpler terms and with examples and photos and references if possible.

Because people like me may not know what are clubed antenna . So it’ll get a hard way for me to get interested in your ants!!

What’s special about crazy ants!?
[1:30 PM, 8/18/2021] Abhijeet Singh: Great!!
I can see the legs of the ants are very shiny or whitish kind of. Is it because of the flash!?
[1:48 PM, 8/18/2021] Abhijeet Singh: We catch mosquitoes by clap trap method by making a hollow
in between both the hands, so that the mosquito is killed (but not smashed). We catch
only those mosquitoes that come to bite us in our house.

So this photo is from the presentation that we made for Cube National meet 2017 I guess(I’ll share that presentation shortly). We can see the white stripes easily on the Aedes mosquito with a black background. @Lydia cUBe Drosphila @Hina Mudgal Kanpur CUBE @KIRAN CUBE @Saida Sayyed @Aswathy Suresh CUBE
VIDEO SHARED BY SEETHALAKSHMI AT 1.59AM
Close up video of ants.
Location : Pazhuvil.
Time : 11:47pm.
Date:16/8/21.
Data collector : seethalakshmi CUBE
In this video ants have slightly blue colour​:roll_eyes:. May be due to flash light ??
This was our presentation in 2017 for mosquito project! @Arunan HBSCE @Hina Mudgal Kanpur CUBE @Lydia cUBe Drosphila @Saida Sayyed @KIRAN CUBE @Seethalakshmy CUBE @Aswathy Suresh CUBE @Batul Pipewala Kolkata
Please start with the objective so that readers’ curiosity is raised.
So do you think this is Nobel winning C elegans? On what basis? @Batul Pipewala Kolkata
So, what will be the approximate size? @Batul Pipewala Kolkata
Excellent
[2:42 PM, 8/18/2021] Arunan HBSCE: No Koel call these days, 3rd week of August, surely! Sorry, we should have been more particular to find out till when Koel calls were there in South Mumbai.
[2:43 PM, 8/18/2021] Arunan HBSCE: Some reference to your location like Chembur,Mumbai @DP HBCSE
Koel cooing
Location : Pazhuvil
Date:18/8/21
Time: 2:03pm
Data collector: seethalakshmi
[2:47 PM, 8/18/2021] Arunan HBSCE: Wow! More information like Authors, Year please @Rahul Kushwaha
[2:56 PM, 8/18/2021] Arunan HBSCE: Don’t you see worker ants carrying some white stuff? Are these not larvae or pupae?
Please look at your photos and tell us more about these.Wgat does references tell you?
[2:56 PM, 8/18/2021] Arunan HBSCE: @Seethalakshmy CUBE
[2:57 PM, 8/18/2021] Arunan HBSCE: Reference please! @Seethalakshmy CUBE
[2:59 PM, 8/18/2021] Arunan HBSCE: Please describe your major problem along with photos, so that readers can help you. Please spell it out. @Seethalakshmy CUBE
[3:01 PM, 8/18/2021] Arunan HBSCE: Don’t you have any reference link? Is it difficult to search for references? What key words you used? What search results you got? @Seethalakshmy CUBE please describe your worry.
[3:02 PM, 8/18/2021] Arunan HBSCE: Do you find it difficult to search for what is Imaginal disk. Please describe where you get stuck in this. @Seethalakshmy CUBE
[3:04 PM, 8/18/2021] Arunan HBSCE: Please give an example of a protein. @Seethalakshmy CUBE And explain how is it made up of @Seethalakshmy CUBE
Are there more nematodes in this slice than what you transferred? @Batul Pipewala Kolkata Please start with your objective with each such picture.
So, do you think C elegans. An be cultivated on raw potato slice with a few drops of milk? @Batul Pipewala Kolkata @Anshu Kadam (Nematodes)
What happened to the Controls, like without milk?@Batul Pipewala Kolkata
What is your objective? Always start with the objective to raise the curiosity of the reader @Aditiya Joshi CUBE What is your interpretation of the observation?

What is your further plan?@Aditiya Joshi CUBE
[3:13 PM, 8/18/2021] Arunan HBSCE: Please start with objective please! @Aditiya Joshi CUBE And what is your interpretation of your observation?
What is your further plan? @Aditiya Joshi CUBE
[3:16 PM, 8/18/2021] Arunan HBSCE: Why? Why should fermentation result in such indicators? @Aditiya Joshi CUBE
[3:17 PM, 8/18/2021] Arunan HBSCE: Why Carbon dioxide in fermentation? @Aditiya Joshi CUBE @Seethalakshmy CUBE
[3:18 PM, 8/18/2021] Arunan HBSCE: What is your expectation? @bunny~~ And why? @Seethalakshmy CUBE @Aditiya Joshi CUBE
[3:21 PM, 8/18/2021] Arunan HBSCE: Very important in Science and Science communication @Abhijeet Singh @Seethalakshmy CUBE Please help clarifying to get collaborators like @Abhijeet Singh
Wow! Please give more on it @Abhijeet Singh
Please start collecting mosquitoes in August till 23rd September when the Day and Night time will be equal, EQUINOX! @Abhijeet Singh @Hina Mudgal Kanpur CUBE @Seethalakshmy CUBE @Aswathy Suresh CUBE and others
No reference for measurement of size?!! @Seethalakshmy CUBE

Size of ant
Size of worker ant in my formicarium.
Size:2.8 mm
Location : Pazhuvil, Thrissur
Date:12/8/21
Time: 6:14
Data collector : seethalakshmi
@Arunan HBSCE sir
Please make dome specific comments @Seethalakshmy CUBE
What is striking in this compilation? @Seethalakshmy CUBE

What do you understand from Gaurangi’s and Ubaid’s findings? @Seethalakshmy CUBE

VIDEOS SHARED BY YASH AT 3.48PM
Today’s Headline - 11th Day update of SLPbA2 sub culture shows 50 fruit flies and few dead fruit flies!
Media in SlPbA2 is little liquidy as compared to SLPbB2 and C2!

Media date - 06/08/2021
Transfer date - 7/08/2021
No of flies transferred - 35

16/08/2021
No of flies -50
No of larvae - 5 - 3mm larvae
No of pupae - 20
No of dead flies - 10 @KIRAN CUBE maam @Arunan HBSCE sir @Aswathy Suresh CUBE @Sidhy Kerala Drosophila @Janhvi Deshmukh
SLPbB2

Today’s Headline - Today is the 11th day of Sub culture and SLPbB2 shows ~ 70 fruit flies with a few unhatched pupae.

Media date - 6/08/2021
Transfer date - 7/08/2021
No of fruit flies Transferred - 30

18/08/2021
No of flies - 70
No of larvae - - 4
No of pupae - 50
@KIRAN CUBE maam @Arunan HBSCE sir @Batul Pipewala Kolkata maam @Aswathy Suresh CUBE @Lydia cUBe Drosphila @Isha Pawle @Janhvi Deshmukh
SLPbC2 *Breaking News Record
Record of ~ 100 Fruit flies seen on the 11th Day of Sub culture in SLPbC2

Media date - 6/08/2021
Transfer date - 7/08/2021
No of fruit flies Transferred - 25

18/08/2021
No of flies - 100
No of larvae - - 8
No of pupae - ~ 20
No of dead flies - 10 @KIRAN CUBE maam @Arunan HBSCE sir @Batul Pipewala Kolkata maam @Aswathy Suresh CUBE @Sidhy Kerala Drosophila @Lydia cUBe Drosphila @Janhvi Deshmukh @Aastha Ahuja Cube
Breaking News Headline
SLPbA1 culture shows Zero flies, 0 pupae and larvae on 21st day!

Media prepared - 24/07/2021
Fruit fly transferred on - 24/07/2021 - One gramina fruit fly was transferred
18/08/2021
No of flies - 0
No of larvae - - 0
No of pupae - ~ 0
@KIRAN CUBE maam @Arunan HBSCE @Batul Pipewala Kolkata maam @Aswathy Suresh CUBE @Sidhy Kerala Drosophila @Lydia cUBe Drosphila @Janhvi Deshmukh @Aastha Ahuja Cube and others
Breaking News Headline
SLPbB1 culture shows 1fruit fly, 0 pupae and larvae on 21st day!

Media prepared - 24/07/2021
Fruit fly transferred on - 24/07/2021 - One gravid fruit fly was transferred
18/08/2021
No of flies - 1
No of larvae - - 0
No of pupae - ~ 0 @⁨Kiran Maam HBCSE⁩ maam @⁨Arunan Sir HBCSE⁩ sir @⁨Batul Ma’am CUBE⁩ maam @⁨Ashwathy CUBE Nattika⁩ @⁨Sidhy CUBE Nattika⁩ @⁨Isha HBCSE⁩ @⁨Lydia HBCSE⁩ @⁨Aastha⁩ @⁨Janhvi Deshmukh⁩ and others
Breaking News Headline
SLPbC1 culture shows 2fruit fly, 5 pupae and 0 larvae on 21st day!

Media prepared - 24/07/2021
Fruit fly transferred on - 24/07/2021 - One gramina fruit fly was transferred
18/08/2021
No of flies - 2
No of larvae - - 0
No of pupae - ~ 5

Summary of Fruit fly Cultures at CUBE Home lab Prabhadevi
SLPbA1 | SLPbB1 | SLPbC1 - Started on 24/07/2021
SLPbA2 | SLPbB2 | SLPbC2 - Started on 7/08/2021

Status of Cultures
Healthy Cultures - SLPb C2 +++
SLPbB2 - ++
SLPbA2 - +

+++ - Most Healthy culture showing plenty of Fruit flies, pupae and few larvae
++ -good culture - showing plenty of Fruit flies, pupae and less larvae

• • Reasonably good culture showing plenty of Fruit flies, few pupae and very less larvae

Bad cultures -SLPbB1 - ×××
SLPbA1 - ××
SLPbC1 - ×
××× - meaning - it Contains no Fruit flies, pupae and larvae with lot of fungal growth
×× - meaning - it Contains 1 or 2 Fruit flies, pupae and larvae with lot of fungal growth
× - Meaning it contains 1 or 2 Fruit flies and few pupae and no larvae with very less fungal growth
@Arunan HBSCE sir @KIRAN CUBEmaam @Batul Pipewala Kolkata maam @Aswathy Suresh CUBE @Lydia cUBe Drosphila @Sidhy Kerala Drosophila @Isha Pawle and others

VIDEO SHARED BY SIDHY AT 4.20PM
Single line culture of fruit flies.

TRSV medium - 15/08/2021, 11:00 PM
Transferred gravid fly on 16/08/2021 , 11:40 AM

3 Single line cultures : In the video , from left – SLTT A , SLTT B , SLTT C. ( SLTT - Single line culture Thanniam Thrissur)
Date of video : 16/08/2021
Location: Thanniam , Thrissur , Kerala.

Immediate objective : Make single line culture stocks of fruit flies.

Short term objectives :

1.Family level identification.
2. Conduct experiments based on observations and hypothesis.
3. Understand the life span of fruit fly.

Long term objectives :

1.Genus and Species level identification.
2. Olfactory sensitivity test.

Sidhy.P.P , CUBE S N College , Nattika.
Single line cultures of fruit flies.
SLTT A , B , C
Started on 16/08/2021
TRSV medium

18/08/2021
Observed ~ 20 small larvae in A and B , and ~ 10 small larvae in C.

Location: Thanniam , Thrissur , Kerala.
Sidhy.P.P , CUBE S N College, Nattika.
Do you think think this is Black Crazy ant? Why?@Seethalakshmy CUBE @Abhijeet Singh

S4E146 of CUBE chatShaala: Difference in the olfaction sensitivity of fruit flies

Webinar Link CUBE (tifr.res.in) Please click on the Link (BLUE) to join the CUBE ChatShaala Timing:5.30 pm to 9.30 pm Today’s session will be followed by a workshop on mosquito by 8pm onwards Kindly add your place/affiliation along with your name while joining Looking forward to your participation Thanks Please click on the below given link if you face any issue in joining the webinar

Kindly click on the above👆🏻 given link for the summary of last discussion.

Invitation to join today’s CUBE chatShaala,
Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in all CUBE what’s app groups and on metastudio after the session

Here’s the direct link of the CUBE webinar: https://webinar.hbcse.tifr.res.in/b/kir-hye-cn4
Note: we have changed the room link to avoid technical error

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
Congratulations to CUBE fruit fly Collaborators.

Sidhy has restarted single line Cultures
Congrats sidhy​:clap:
Kamakhya Temple reopens for devotees today amid COVID pandemic

Kamakhya Temple reopens for devotees today amid COVID pandemic

Source : “The Sentinel” via Dailyhunt

Download Now
http://dhunt.in/DWND
@Yash Sheregare CUBE all flies in SLPbA1 are died ??

PHOTO SHARED BY BATUL AND SEETHALAKSHMI AT 8.41AM
Update on Ant Model system…
They store sugars here.
Location : Pazhuvil.
Time : 6:44 pm.
Data collector : Seethalakshmi. C. R.

[KiranKalakotiR (Kiran Kalakoti)]

18th August 2021, 9.30pm to 19th August 2021, 9.30pm
Renamed the Cultures

In July 2020 , I had made 5 single line cultures. They were named as SLTT-A , SLTT-B, SLTT-C , SLTT-D and SLTT-E . After losing all these cultures , again started 2 new single line cultures in February 2021. To distinguish these cultures from 2020 cultures , named them as SLTT21-A and SLTT21- B. These two cultures lost in April 2021.

On 16 August 2021 , started 3 new single line cultures. And named them same as that of 2020 cultures. This will make confusions and affect the data of almost six months long single line cultures of 2020 which were also identified as of Drosophilidae family. I didn’t think this while naming. I thought that 2020 cultures are not existing and now I am starting the cultures after a gap of three and a half months.

In today’s chatshaala ,Arunan Sir asked me about this and made me understand the importance of nomenclature in research projects . Also I realised how valuable my old works and findings even when the cultures are not existing.

So , renamed all the single cultures of 2021 as a continuation of 2020 single line cultures. New names are :

SLTT - F ( SLTT21 - A) February
SLTT - G ( SLTT21 - B) February

SLTT - H 16 th August
SLTT - I 16 th August
SLTT - J 16 th August

@Arunan HBSCE Sir , @Lakshmy PJ CUBE , @KIRAN CUBE Ma’am , @Aswathy Suresh CUBE @Abhijeet Singh helped me in renaming and understanding the importance of nomenclature in projects. Thank you everyone.
Renamed the single line cultures of 16 th August 2021 as SLTT-H , SLTT-I , SLTT- J.

VIDEO SHARED BY SEETHALASHMI AT 11.54PM
Video of black crazy ants foraging on a sugar bait in Fiji, Vimeo video (© Eli Sarnat, Creative Commons Attribution, Share Alike CC BY-SA Licence)
I think my name is missing. Aditya Joshi, Kalyan, Maharashtra
Reference :

http://www.piat.org.nz/index.php?page=black-crazy-ant
This ant similar to my Formicarium Ant
Ok …I will add
VIDEO SHARED BY SEETHALASHMI AT 12.02AM
My formicarium ant. Location : Pazhuvil. Date: 13/8/21. Time: 12:04 am. Data collector : Seethalakshmi
Ants body shows slightly blue colour with flash light.
517 th day of CUBE ChatShaala: 18th August 2021.

19 CUBists from 19 Centres had joined the webinar.

INDIA

Delhi
Aastha Ahuja: ANDC
Dr P. Chitralekha: DSC

Haryana
Aashi: Panipat

Jharkhand
Morabadi, Ranchi: Rechel Tirkey

Kerala
Aswathy Suresh: Thriprayar
Lakshmy PJ: Kandassankadavu
Seethalakshmi CR: Pazhuvil, Trissur
Sidhy PP: Thanniam, Thrissur

Tamil Nadu
Meena Kumari: Chennai

Maharashtra
Aditya Joshi : Kalyan
Abhijeet Singh: Ulwe, Navi Mumbai
Arunan MC: Breach Candy, South Mumbai
Isha Pawle: Worli
Saida Sayyed: Bandra, Mumbai
Shraddha Sonavane: Panvel
Shivam Kumar Sriwas: Kalyan

Uttar Pradesh
Kiran Yadav: Moradabad
Aarti Vankatesan: Vigyanshaala
Divyanshi Chauhan: No…

Black crazy ants.
Thanks @Shraddha Sonavane CUBE

Iam just trying to take photos of my formicarium Ant. Location : Pazhuvil. Time : 2:20 pm. Date :30/5/21.Data collector : Seethalakshmi

Ant Life Cycle | Ask A Biologist

SUMMARY - CUBE Chatshaala.
18.08.2021

Single Line Culture updates @Sidhy from from Thanniam - Thrissur Kerala, Restarted fruit flies works on 16-08-2021 made new single line cultures.

There is an issue while naming the cultures.Because last year July 2020 also started 5 cultures named SLTT A, B, C, D & E. But unfortunately she lost those all culture after 6 months later due to some other reasons (Goof ups)

Then February 2021 again restarted 2 single line cultures named SLTT 21A, SLTT 21B that’s also lost after 2month later.

August 2021, made 3 new single line cultures started with one mother flies naming again same that she added in pervious single line culture bottles.

Why Naming Is Important!?

Importance of Nomenclature.

Whatever work we are doing or done in at particular model systems that all works were recorded in here or somewhere. So all details are valuable even names also important factors that will help to identify.

Aashi from Haryana were joined yesterday chatshaala.
She want to addressing about how to study microbes from various corner of home. Eg : microbes on fridge handle.

Is there any effects of sunlight in microbes.?

Difference between sunlight and some other lights, how will effects microbes!

Why only sunlight get effects microbes?

Culture microbes at homes!
How can we culture microbes at homes.

What type of media will help to grow microbes?

Why can’t use POTATO slice for to isolate microbes.

Why microbes growing very well on potato surface ? What could be the season?

Potato contains STARCH, Carbohydrates, Irons, Vitamins etc.

What is the factors that promoting microbes growth over potato slices?

STARCH

Starch is contains carbohydrates. Starch is a polymer of glucose C6 H12 O6. Complex Carbohydrates.

Importance Culture Media
Which type of Culture media selecting for to culturing microbes?

Aashi Batul Adithya Aastha please add your points
Microbes are present over the surface of potato slices Picture credit : Batul

@Aditiya Joshi CUBE Experiment isolating microbes from using coconut water.

Experiment details giving below

“18/8/2021. The fermented coconut water was spread on the boiled potato slices. Method: boiled potato slices were taken in a container with lid. A ear bud was used to take the fermented coconut water from the bottles. One bud for open bottle and one for closed bottle. The bud was dipped/soaked in the fermented coconut water. This bud was then spreaded on potato in S shape. Marked red are inoculated slices with open bottle coconut water. Marked blue are slices with closed bottle coconut water. Marked green are the non inoculated potato slices. Expectation: No colonies seen on non inoculated potato slices. Colonies should appear on inoculated slices. Date: 18/8/2021. Aditya Joshi. Kala Talav, Kalyan west.”

@Arunan HBSCE sir @Aditiya Joshi CUBE @Abhijeet Singh @Aashi @Batul Pipewala Kolkata @Aastha Ahuja Cube

VIDEOS SHARED BY ASWATHY AT 1.51AM
SLBK A22

Update : Fruit Flies
subculture started date : 12.08.2021

Transfer Flies : 1 Fly, Gravid one

Expectations :
18.08.2021 - 6th Day
~40 Eggs
~30 Larvae
~20 Pupae

Observation : 6th Day
18/08/2021
No of Eggs : ~6
No of Larvae : 0
No of Pupae : 0
No of Flies : 1 (Transferred one)

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
SLBK B21

Update : Fruit Flies
subculture started date : 12.08.2021

Transfer Flies : 12 Flies
Gravid Flies : 5, Male Flies : 7

Expectations : 6th Day
18.08.2021

~70 Eggs
~45 Larvae
~40 Pupae

Observation : 6th Day
18/08/2021
No of Eggs : ~30
No of Larvae : 5
No of Pupae : ~69
No of Flies : ~2 (Transferred flies)
Dead Flies : ~10

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
SLBK E17

Update : Fruit Flies
subculture started date : 12.08.2021

Transfer Flies : 14 Flies
Gravid Flies : 6, Male Flies : 8

Expectations :
18.08.2021 : 6th Day
~80 Eggs
~50 Larvae
~40 Pupae

Observation : 5th Day
18/08/2021
No of Eggs : 40
No of Larvae : 25
No of Pupae : 10
No of Flies : ~14 (Transferred flies)
Dead Flies : 0

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
SLBK E16

Update : Fruit Flies
subculture started date : 19.07.2021

Transfer Flies : 30 Flies
Gravid Flies : 9, Male Flies : 21

Expectations :
18.08.2021 : 31st Day
(One month old culture)
~150 Eggs
~80 Larvae
~160 Pupae

Observation : 31Day
18/08/2021
No of Eggs : ~150+
No of Larvae : ~20
No of Pupae : ~170+
No of Unhatched Pupae : ~60+
No of Flies : ~120+
Dead Flies : 40+

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
BREAKING NEWS
SLBK E15 - 47 Days old subculture bottle have ~100 Eggs, ~160 Pupae, ~30 Flies, Media still solidified condition.

SLBK E15

Update : Fruit Flies
subculture started date : 02.07.2021

Transfer Flies : 15 Flies
Gravid Flies : 8, Male Flies : 7

Expectations :
18.08.2021 : 47 Days old culture bottle
~200 Eggs
~80 Larvae
~50 Pupae

Observation : 47 Day
18/08/2021
No of Eggs : ~100
No of Larvae : ~8
No of Pupae : ~160
No of Unhatched Pupae : ~60
No of Flies : ~30
Dead Flies : ~60

Media solidified

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
[9:22 AM, 8/19/2021] Batul Pipewala Kolkata: Wow!
[9:23 AM, 8/19/2021] Batul Pipewala Kolkata: There are 100s of nematodes in this culture which was started with 2to 3 nematodes
[9:24 AM, 8/19/2021] Batul Pipewala Kolkata: Yes C elegans can be cultured on raw potato slice with a few drops of milk added on the first day
[9:25 AM, 8/19/2021] Batul Pipewala Kolkata: Approximate size of the nematodes is 1.5 to 2mm
[9:26 AM, 8/19/2021] Batul Pipewala Kolkata: No this is not C elegans because the middle bulb is not completely round as given in references

Cardamine species at CUBE HomeLab, Bhulabhai Desai Road, South Mumbai 19, August 2021 Photo: mcarunan let’s follow up with data from collaborators from all CUBE centers.
A close relative of the model system, Arabidopsis for plant sciences research.https://www.ceplas.eu/en/research/principal-investigators/prof-dr-miltos-tsiantis/

I think a fruit fly visited my setup to taste toddy… Open bottle of fermenting coconut water. 19/8/2021. Kala Talav, Kalyan west.

3rd day of coconut water fermentation… We can see a whitish layer settled at the bottom of bottles. The layer gets disturbed when swirled. A thin white layer is present on surface of coconut water too. 19/8/2021. Kala Talav, kalyan west.
Phyllanthus growing well in the pots. Kala Talav, Kalyan west.
This phyllanthus in pot2 have produced many branches. Its flowering too… But not producing a fruit. What can be the reason🤔 @Lakshmy PJ CUBE @Sunita Chahar CUBE @Shraddha Sonavane CUBE @Seethalakshmy CUBE @Arunan HBSCE @P.chitralekha and all others. Kala Talav, Kalyan west. 19/8/2021.

VIDEO SHARED BY ARUNAN AT 10.59AM
Date:5/08/21.Time :11 31am.seed bursting in cardamine. A small touch is enough to break the matured seed pods of cardamine. Arunima CUBE Home lab Kozhikode Kerala.
[11:50 AM, 8/19/2021] Aswathy Suresh CUBE: Larvae that I found in culture after 6th day. Most of the larvae I observed it was 3rd instar stage.1st instar & 2nd instar larvae couldn’t able to find out inside culture.

First pupae observed in E culture on 6th day, ~10 pupae formed inside culture on 18/08/2021.

Observations Based Analysis :

12.08.2021 - Transferred flies 14 flies, 6 gravid flies, 8 male flies.

13.08.2021 - No eggs.

14.08.2021 - No eggs, No Larvae observed.

15.08.2021 - No eggs, No Larvae observed.

16.08.2021 - Eggs ~10 , No Larvae, No Pupae observation.

17.08.2021 - Eggs ~30 , No Larvae, No Pupae

18.08.2021 - Eggs ~80 , 45 more larvae (Different stage) mostof them observed below the media side, 10 Pupae.
[11:55 AM, 8/19/2021] Aswathy Suresh CUBE: Expecting eggs inside the A22 culture. Because I transferred only one fly that reminded in A21 culture. Look like it is gravid one that’s why Iam expecting some eggs will laying that one fly inside in A22 culture.
[11:58 AM, 8/19/2021] Aswathy Suresh CUBE: Yes sir, I missed larvae inside E16 culture bottles. Because I am not much expected any larvae has remained after 1 month old bottles.
[11:59 AM, 8/19/2021] Aswathy Suresh CUBE: Collaboration through helping find out things that we can’t able see it but others can.
[11:59 AM, 8/19/2021] Arunan HBSCE: What is your expectations now, from tomorrow onward, in this bottle? That will help you to understand what is good or bad about your your culture conditions of Single Line E flies which were in very good shape so far.
Don’t you think so? @Aswathy Suresh CUBE
What are your comments @Yash Sheregare CUBE @Lydia cUBe Drosphila @Aastha Ahuja Cube @Batul Pipewala Kolkata and others
[12:01 PM, 8/19/2021] Arunan HBSCE: Why what is the basis for that? Is a culture of one month is too old? @Aswathy Suresh CUBE
What evidence we gave for that?
[12:04 PM, 8/19/2021] Arunan HBSCE: What is the best time (after how many days) to transfer flies for subculture from old bottles to fresh culture bottles? @Aswathy Suresh CUBE @Yash Sheregare CUBE @Aastha Ahuja Cube
[12:06 PM, 8/19/2021] Arunan HBSCE: if flies are thriving in
such bottles, don’t they mate and lay eggs and the eggs hatch to larvae? What makes you think that there can it be larvae? @Aswathy Suresh CUBE @Yash Sheregare CUBE
Is this not a nice Goof- up to celebrate so thate every one realizes that goof ups are common when we do research.
And such realization very important for progress in science! @Aswathy Suresh CUBE @Lydia cUBe Drosphila @Yash Sheregare CUBE @KIRAN CUBE
Expectations about E17 culture.

Expectations today onwards :

19.08.2021 - ~100 eggs + ~60 Larvae + ~25 Pupae

20.08.2021 - ~150 eggs + ~70 Larvae + ~30 Pupae

21.08.2021 - ~200 eggs + ~85 Larvae + ~50 Pupae + ~10 New flies + ~14 Old flies.
Monsoon plants… Raanbhendi/(wild okra)… Beautiful yellow flowers… Often seen growing in monsoon along with roadsides, gardens, backyards, railway tracks… The flowers, buds n small tender okra can also be seen here. Kala Talav, Kalyan west. 19/8/2021. Ate these seen in other parts of mumbai n India…? Lets find…
[12:23 PM, 8/19/2021] Arunan HBSCE: Sorry, without other essential information like the Date of Transfer into SLBT E17 bottles, this write up does not communicate much @Aswathy Suresh CUBE
Please repost so that others will be inspired to comment and become collaborators.
Please repost essential information. @Aswathy Suresh CUBE
[12:25 PM, 8/19/2021] Arunan HBSCE: Do you have larvae, pupae and adults today, 19 August 2021, in this bottle?@Aswathy Suresh CUBE
Please post evidence too.@Aswathy Suresh CUBE
Photo with plane white background.

Sir, I think this image will more better when shape of leaflets are clearly visible.
It will be help in identification of Cardamine
Ohh!! Toddy lover Fruit fly😋
Wow!!! @Aditiya Joshi CUBE Your phyllanthus plants are grown up.
Great @Aditiya Joshi CUBE
What is your next plan?
Can you please show the below part of the branches.There may be fruits produced. Later they fall off @Aditiya Joshi CUBE
[12:33 PM, 8/19/2021] Arunan HBSCE: @Abhijeet Singh Please continue commenting on a posts.
[12:36 PM, 8/19/2021] Arunan HBSCE: on all*
What about other plants?
Are they fruiting?
[12:51 PM, 8/19/2021] Aswathy Suresh CUBE: Not much old culture bottle is that.it Is just passed one month. Most of my observation after around one months bottles have less number of larvae. That’s why I mentioned I like that.
[12:51 PM, 8/19/2021] Aswathy Suresh CUBE: 20-25 is the Best time for to transfer flies in to new culture media bottles.
20-25 Days in normal situation (Good number of Larvae, Pupae, Adult flies).

But is there any emergency situation time come, don’t wait for that much time. immediately need to transfer those flies in new media bottle in order to save those flies.

PHOTO SHARED BY LAKHSMI AT 12.58PM
Long term objective -Floral dip method
Short term objective -

1. To observe life cycle
2. observing heteroblasty(To observe the transformation from simple to compound leaves.
   3)Identifying cardamine plants

Date:16/8/2021
Time:5:38pm
Place: Mampilly
Cotyledonary stage+ 4 leaf stage.
All are simple leaves

Phyllanthus urinaria mature fruits collected on 15/8/21
date of photo–19/08/21. Objective- To study Seed germination in Phyllanthus plant.
location- Panvel Photo taken by- Shraddha
Date:16/8/2021
Time:5:38pm
Place: Mampilly
[1:01 PM, 8/19/2021] Aditiya Joshi CUBE: I think my fruits fall off… Only some parts are seen fallen in soil…
[1:02 PM, 8/19/2021] Aditiya Joshi CUBE: Yes. Others plants in pot3 are fruiting
Please let us know how many fruitflies you have now! From all the cultures that are doing good!

I think SlPb A2/B2/C2 are doing good!

What’s your further plan @Yash Sheregare CUBE !!!
Please post updates of all culture bottles of SLBK E to show what is the right type of culture and what is not good culture and why @Aswathy Suresh CUBE
Let’s all get lessons from this. @Aswathy Suresh CUBE
Great ! @Shraddha Sonavane CUBE How many seeds in each fruit?
Yes @Lakshmy PJ CUBE I’ll post photos. I have seen flowers falling off
So @Sidhy Kerala Drosophila how are your cultures doing in order to achieve your Immediate term objective!?
How would you comment on it?
Let us know what are the difficulty you are facing now! Let’s discuss it out!
@Aswathy Suresh CUBE @Lydia cUBe Drosphila @Yash Sheregare CUBE
BREAKING NEWS SIMPLE TO COMPOUND LEAF TRANSFORMATION.Date:18/8/2021
Time:5:23pm
Place: Mampilly
Cotyledonary stage+ 4 leaf stage
The 4th simple leaf split in to compound leaf

White shiny thread like structure observed on dry stem of Phyllanthus urinaria plant. What can it be? Date of photo-19/08/21 location-Panvel data collected by-Shraddha @Aditiya Joshi CUBE @Arunan HBSCE Sir @Batul Pipewala Kolkata mam @Aswathy Suresh CUBE @Seethalakshmy CUBE @Aashi & others…
Yess that would be great!
Let’s start it doing together!
@Aswathy Suresh CUBE @Hina Mudgal Kanpur CUBE @Lakshmy PJ CUBE @Seethalakshmy CUBE
Please comment and let’s plan!!
[1:12 PM, 8/19/2021] Aditiya Joshi CUBE: Same @Shraddha Sonavane CUBE
[1:12 PM, 8/19/2021] Aditiya Joshi CUBE: I’ll post photos
Date:19/8/2021
Time:1:11pm
Place: Mampilly
Cotyledonary stage+ 4 leaf stage
The 4th simple leaf split in to compound leaf
5th leaf emerging
Date:19/8/2021
Time:1:11pm
Place: Mampilly
Cardamine plants growing healthy.
Looks like fungi to me

White thread like growth on dried stems… Kala talav, Kalyan west.@Shraddha Sonavane CUBE

White thread like growth seen on dried stems.
19/8/2021
I hope in this photo white shiny threads are clearly visible.& I can see some yellow spores in orange colour circle
Flowers have fallen fron the leaves @Lakshmy PJ CUBE @Shraddha Sonavane CUBE
Yes…it’s similar

Dried flowers underside of leaves. Pot2 phyllanthus.
Wow!! This video is so amazing! We can see the white stripes on the back of ants.
Please comment on how do we stand to our claim that the ants we have are Black Crazy Ants too! @Seethalakshmy CUBE
Wow great @Shraddha Sonavane CUBE Does these fruits burst out?
Please keep it in closed container. Otherwise they will burst out and disperse seeds.
Yes I think that they look similar from the once you sent before!
I think 3 seeds in single capsule
Is that root hairs @Shraddha Sonavane CUBE
[1:26 PM, 8/19/2021] Lakshmy PJ CUBE: Which means they are fungus? @Shraddha Sonavane CUBE
[1:26 PM, 8/19/2021] Lakshmy PJ CUBE: @Aditiya Joshi CUBE
[1:26 PM, 8/19/2021] Shraddha Sonavane CUBE: Most of fruits are turned brown but not burst out.

Yes, kept it in closed container
[1:27 PM, 8/19/2021] Shraddha Sonavane CUBE: No @Lakshmy PJ CUBE .
There are on stem
Is it because of any Pest attack @Aditiya Joshi CUBE
[1:28 PM, 8/19/2021] Shraddha Sonavane CUBE: & on branches
[1:29 PM, 8/19/2021] Shraddha Sonavane CUBE: I don’t have any idea. I have to check
[1:31 PM, 8/19/2021] Shraddha Sonavane CUBE: Mam, is it because of white shiny threads like appearance?
Actually no insect or pest are seen on plant. Its producing new branches n sprouting new leaves… Flowers too… But no fruiting yet…
Can it be a mealybug infestation? @Shraddha Sonavane CUBE @Lakshmy PJ CUBE @Aditiya Joshi CUBE
I too had observed similar white structures on the Phyllanthus plant that I had brought from my building compound. And the leaves too had wilted out…that resulted in drying up of the whole plant.
Wooooooo A22 Bottle
That’s amazing this is your 23rd Bottle of A series. @Aswathy Suresh CUBE you have been doing a wonderful efforts.

But why this bottle is not fulfilling your expectations?
Wooow you got good number of Pupae but why flies are drying.

10 dead flies in 6 days
But there is no mealy bug attack on my Phyllanthus plants yet.
Nice photo with a nice background.

Is this an invasive species or local Cardamine of Mumbai?

What species of Cardamine this could be?
@Shraddha Sonavane CUBE @Arunima SN Nattika Cardamine @Lakshmy PJ CUBE @Batul Pipewala Kolkata and other Cardamine collaborators.
How do we indentify the species of Cardamine?
I think we should check… it can be a possibility of fungi … because fungi can grown on dry & decay matter too.
What you think @Aditiya Joshi CUBE @Lakshmy PJ CUBE @Drishtant Cube Moina
Another Cardamine from another place of India.

Is this the munnar variety? Or plant brought from local plant nursery?

Which species of Cardamine this could be?
Wooowww another Cardamine but from which place of India?
@Lakshmy PJ CUBE did you find them in your surroundings or you got these Cardamine from plant nursery?
What is the expectation? When do you expect to observe floral buds in these Cardamine plants?
If this is the case, then did @Aditiya Joshi CUBE observe the white structures on dried up plants or green, healthy Phyllanthus plants?
In how many days after the seed germination does this compound leaf has arises? What do you mean by transformation? Was this leave simple before?
Dried stems… dead plants @Drishtant Cube Moina
So, let’s rule out either of the possibilities that we have thought of; Fungi or mealybugs?
What do Fungi normally need to grow/sustain? The same question goes for mealybugs.
Any more inputs to add to the protagonists?
Absolutely sir!

PHOTOS SHARED BY ABHIJEET AT 2.16PM
Fungus going on a wooden block in my balcony. Amazing site!! Date:- 19/08/2021, Time: 2.15pm, Location:- Ulwe, Navi Mumbai, Maharashtra.
Green once are:- Trichoderma Trichoderma - Wikipedia … Google lence application suggests! Date:- 17/08/2021, Time:- 2.15pm, Location:- Ulwe, Navi Mumbai, Maharashtra.
White once are :- Crepidotus https://en.m.wikipedia.org/wiki/Crepidotus Time:- 2.15pm DATE:- 19/08/2021. Location:- Ulwe, Navi Mumbai, Maharashtra.

VIDEOS SHARED BY ASWATHY AT 2.17AM
SLBK A 22 - single line sub culture bottle of A Line… Date of transfer flies 12.08.2021.

Expectations on 7th Day
19/08/2021
~50 Eggs
~40 Larvae
~30 Pupae

Observations on 7th Day
19/08/2021
~40 Eggs
~ 2 Larvae
~ 0 Pupae
1 Flies (Transferred)

Update : Aswathy Suresh CUBE SN College Nattika.
Home Lab : Bottukadavu - Thrissur Kerala.
SLBK B21 - single line sub culture bottle of B Line… Date of transfer flies 12.08.2021.

Expectations on 7 Day
19/08/2021
~80 Eggs
~55 Larvae
~50 Pupae

Observation : 8th Day
19/08/2021
No of Eggs : ~50
No of Larvae : ~10
No of Pupae : ~70
No of Flies : ~7
Dead Flies : ~10

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur Kerala
SLBK E17 - single line sub culture bottle of E Line… Date of transfer flies 12.08.2021.

Expectations on 7 Day
19/08/2021
~80 Eggs
~55 Larvae
~50 Pupae

Observation : 8th Day
19/08/2021
No of Eggs : ~60
No of Larvae : ~30
No of Pupae : ~72
No of Flies : ~14
Dead Flies : ~0

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur Kerala

Pre - Pupae (Larvae turned in Pupae Condition) Picture taken from SLBK E17 subculture bottle.
I am sure there must be more larvae with such number of adult flies! @Yash Sheregare CUBE
Don’t you expect? @Aswathy Suresh CUBE @Aastha Ahuja Cube @Abhijeet Singh
Any explanation? @Yash Sheregare CUBE @Aswathy Suresh CUBE
When did you last see any larvae, pupae or adult fly in this bottle? @Yash Sheregare CUBE
Excellent summary @Yash Sheregare CUBE
Excellent write up! Please give a BREAKING NEWS? @Sidhy Kerala Drosophila
No fly? And when do you expect Pupae? @Sidhy Kerala Drosophila
How do you describe the features of these ants? @Seethalakshmy CUBE
Why not take photos along with some reference Scale? @Seethalakshmy CUBE
[3:33 PM, 8/19/2021] Arunan HBSCE: Photo of eggs please ! @Aswathy Suresh CUBE
[3:44 PM, 8/19/2021] Arunan HBSCE: Middle bulb? Is it how this structure is described in references? Please give reference. @Batul Pipewala Kolkata
What causes the beginning of compound leaves in Cardamine? @Lakshmy PJ CUBE @Arunima SN Nattika Cardamine
Any differential expression of genes implicated for starting the development of Compound leaf from Simple Leaf state? @Lakshmy PJ CUBE @Arunima SN Nattika Cardamine @KIRAN CUBE @P. Chitralekha Cube JNU @Shyam T.G Cube SN Nattika Kerala
[3:53 PM, 8/19/2021] Arunan HBSCE: Any new gene expression? @Lakshmy PJ CUBE
[3:55 PM, 8/19/2021] Arunan HBSCE: Any reference? @Shraddha Sonavane CUBE
[3:57 PM, 8/19/2021] Arunan HBSCE: @Arunima SN Nattika Cardamine Please respond.

S4E149 CUBE chatShaala: How to name and label experimental set ups?

Yesterday in the session we had the context to understand how to name and label experimental set ups. @sidhypp one of the fruit fly CUBE collaborators has restarted the single line cultures of fruit flies all over again but the name of these new bottles are same as previous ones. CUBE ROOM LINK Please click on the CUBE (BLUE) to join the CUBE ChatShaala Timing:5.30 pm to 9.30 pm Kindly add your place/affiliation along with your name while joining. Please click on the below given link if yo…

Kindly click on the above👆🏻 given link for the summary of last discussion.

Invitation to join today’s CUBE chatShaala,
Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in all CUBE what’s app groups and on metastudio after the session

Here’s the direct link of the CUBE webinar: https://webinar.hbcse.tifr.res.in/b/kir-hye-cn4
Note: we have changed the link to avoid technical error

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.

Pseudomelanism.

In a world first case ,this zebra , born in Kenya’s Mara Reserve with unique polka dot appearance

A rare genetic mutation in which animals display some sort of abnormality in their stripe pattern,zebra foal with a dark coat and white polka dots has been spotted in Kenya’s Masai Mara National Reserve.
Data taken from : National geographic 2/9/2019.
Data collected by: Theertha
CUBE SN Nattika.

Genetic change results in unusual zebra foal - Genome BC

VIDERO SHARED BY MAN AT 6.27AM
CUBE Ranchi ReMan’s Home Lab 19-08-2021
Fruitfly Model System Update

Immediate Objective: to start a single line fruitfly culture(RNSL-B) at ReMan’s Lab in TRSV Media

Short Term Objective: -To identify the trapped fruitflies
-To get native Drosophila melanogaster species

Media prepared- 18-08-2021
Transferred date- 19-08-2021

Activity
-Transferred a gravid fly in a TRSV media bottle, 24 hours after media preparation
-Tagged the bottle as RNSL-B

Expectation
-New flies will emerge on 27th Aug, 2021
Analysis of circadian leaf movement rhythms in Arabidopsis thaliana - PubMed.

Analysis of circadian leaf movement rhythms in Arabidopsis thaliana
Great @Man Masih Beck RANCHI CUBE @Rechel Turkey Cube Ranchi Moina
When did you started this single line culture ?
How many single line cultures you made?
What is your objective?
@Man Masih Beck RANCHI CUBE @Rechel Turkey Cube Ranchi Moina
I transferred one gravid fruitfly in TRSV media bottle to prepare one single line (RNSL-B).
The immediate objective is to get successful in maintaining the culture as well as observe it’s lifecycle.
Cardamine with caterpillar 13 February 2020

13 January 2020 How Shraddha Sonawane story of Host specificity of butterfly

7 Feb2020 HBCSE, TIFR Butterfly & Host plant studies, looks like.

[KiranKalakotiR (Kiran Kalakoti)]

19th August 2021, 9.30pm to 21st August 2021, 9.30pm

19 CUBists from 19 Centres had joined the webinar.

INDIA
Delhi
Aastha Ahuja: ANDC
Dr P. Chitralekha: DSC

Haryana
Aashi: Panipat

Jharkhand
Rechel Tirkey: Morabadi, Ranchi

Kerala
Aswathy Suresh: Thriprayar
Lakshmy PJ: Kandassankadavu
Seethalakshmi CR: Pazhuvil, Thrissur

Maharashtra
Aashutosh Mule: Ghatkopar, Mumbai
Abhijeet Singh: Ulwe, Navi Mumbai
Aditya Joshi: Kalyan
Arunan MC: Breach Candy, South Mumbai
Mandar Chavan : Andheri, Mumbai
Saee Kamat: Fergusson, Pune
Shraddha Sonavane: Panvel

Uttar Pradesh
Kiran Yadav: Moradabad
Kanchan Bafila: Pithoragarh

West Bengal
Batul Pipewala: Kolkata

Meena
Prachi Pant

Note
Cubist(s) who had joined today but their name isn’t included in the list, kindly replied to this message.
After 4th Compound leaf, First floral buds was observed. date of photo-31 Jan 2020. location- G6 lab, CUBE HBCSE,Mumbai. Photo taken by- Shraddha Sonavane
The crazy ant worker is relatively small (2.3-3 mm). The head, thorax, petiole, and gaster are dark brown to blackish (Creighton, 1950); the body often has faint bluish iridescence. The body has long, coarse, scattered, suberect to erect, grayish or whitish setae (hair-like projections).

https://entnemdept.ufl.edu/creatures/urban/ants/crazy_ant.htm
Yes sir, The reference ( published by orkin canada) says this

The larvae then shed their skin to become pupae, which look like white adult ants but with their legs and antennae folded up against their body.

Ants | Facts & Identification, Control & Prevention

ants but with their legs and antennae folded up against their body.

Ants | Facts & Identification, Control & Prevention

Meet the BLACK CRAZY ANTS (Paratrechina longicornis) │ Pest or Neighbors?

@Arunan HBSCE, @Man Masih Beck RANCHI CUBE, @Batul Pipewala Kolkata ma’am, @KIRAN CUBE ma’am, @Aswathy Suresh CUBE, @Lakshmy PJ CUBE, @Shraddha Sonavane CUBE, @Shraddha Sonavane CUBE, @Abhijeet Singh, @Vishnupriya CUBE

VIDEO SHARED BY SEETHALAKSHMI AT 1.02AM
Iam observed a big ants in their colony.
Location : Pazhuvil.
Time :11:40 pm.
Date:16/8/21
Data collector : seethalakshmi
[1:06 AM, 8/20/2021] Seethalakshmy CUBE: More videos about it
Loction : pazhuvil
Date:16/8/21
Time:11:40 pm
[1:11 AM, 8/20/2021] Seethalakshmy CUBE: Yes, first we try to find the breeding places of mosquitoes
[1:14 AM, 8/20/2021] Seethalakshmy CUBE: Yes This my major problem
How can check it’s morphology?
Is it possible by using mobile microscopy
[1:16 AM, 8/20/2021] Seethalakshmy CUBE: Yes, we are know but we need scientific evidence
Including their morphology.
That is essential for convincing others
[1:18 AM, 8/20/2021] Seethalakshmy CUBE: That’s very essential for my ants study
Please help me plan this
How can check its morphology???
@Batul Pipewala Kolkata ma’am @Arunan HBSCE sir, @KIRAN CUBE ma’am, @Aditiya Joshi CUBE, @Lakshmy PJ CUBE, @Aswathy Suresh CUBE, @Abhijeet Singh, @Man Masih Beck RANCHI CUBE
And others
[1:19 AM, 8/20/2021] Seethalakshmy CUBE: Ok sir. I will take
[1:29 AM, 8/20/2021] Seethalakshmy CUBE: Black crazy ants
Kingdom:
Animalia
Phylum:
Arthropoda
Class:
Insecta
Order:
Hymenoptera
Family:
Formicidae

Body division : head, Thorax abdomen
Long - antenna and long 6 six legs.
Its common name arises from its characteristic erratic and rapid movement, and habit of not following trails as often as other ants
Habitat
Nesting : damaged trees, soil, indoors
Food: Sugar, sweety substances, insects
Colour : brownish black
Size of workers :2.3-3mm
Non - clubbed antenna ( hair like )

How to introduce model systems in a simple way that every people can understand, get inspired and do some works on it. Specially who are join in Chai & Why section, even non biologists.

Today we were discussed about model systems.
Phyllanthus
Nematodes

But we covered other areas like Butterfly - Plant interaction, Cardamine (Gene expression).

@Lakshmy PJ CUBE @Shraddha Sonavane CUBE @Aditiya Joshi CUBE mentioned about Journey about when did started doing work in CUBE.

Story of Butterfly’s studies done in HBCSE Campus (Butterfly - Plants interaction study done by cubits @KIRAN CUBE @Yash Sheregare CUBE @Drishtant Cube Moina @Lydia cUBe Drosphila …& others)

Psyche butterfly Larvae & Eggs were found in cardamine plants.

7 Feb2020 HBCSE, TIFR Butterfly & Host plant studies, looks like.

Cardamine Leaves stage study
Research Q : Floral dip method in Cardamine flowering bud.
Which leaves stage cardamine flowering bud will form in order to study floral dip method.
4-6 stages of leaves were showing flowering. Total 45 to 50 days taken for to get first floral bud in cardamine plants.

Gene Expression in Cardamine plants.
KNOX Genes : Compound leaves formation because of KNOX genes expression.
Simple leaves change in to compound leaves.

One of other Gene expression example is Moina. Body color change in White to Red. This condition called Hypoxia Condition. Happen in low oxygen levels (Unfavorable conditions)

What are the exact process of gene expression in cells ?

Before that we need to know what is Gene? Where can gene present inside nucleus!.
Tryingto find out this kind of fundamental questions answers through communication.!
Yesterday Discussion Based Reference Given in CHATBOX.

Analysis of circadian leaf movement rhythms in Arabidopsis thaliana Arabidopsis thaliana is the model organism for the study of the higher plant circadian clock. The physiological change in position of young leaves and cotyledons in Arabidopsis seedlings reveals an overt circadian rhythm.
Analysis of circadian leaf movement rhythms in Arabidopsis thaliana - PubMed.

Although Arabidopsis lacks the pulvinus, it displays rhythms in cell elongation and, thus, in growth rate. For example, there is a circadian rhythm in the elongation rate of the abaxial and adaxial cells of the petiole that confers an oscillation in position of cotyledons and leaves (Engelmann and Johnsson, (1998)).circadian rhythm Arabidopsis - Google Search

Analysis of circadian leaf movement rhythms in Arabidopsis thaliana - PubMed.
Analysis of circadian leaf movement rhythms in Arabidopsis thaliana

Life History of the Psyche

https://www.semanticscholar.org/paper/KNOX-genes%3A-versatile-regulators-of-plant-and-Hay-Tsiantis/3a4eaa60fa0951d978689e116fbb1dd4004738cf/figure/7
Interesting picture on Knox gene

A developmental framework for dissected leaf formation in the Arabidopsis relative Cardamine hirsutashow that lateral leaflet formation in C. hirsuta requires the establishment of growth foci that form after leaf initiation. A developmental framework for dissected leaf formation in the Arabidopsis relative Cardamine hirsuta - PubMed

Class I KNOTTED1-like homeobox (KNOX) proteins also promote leaflet initiation in C. hirsuta, and here we provide evidence that this action of KNOX proteins is contingent on the ability to organize auxin maxima via the PINFORMED1 (PIN1) auxin efflux transporter
Summary of CUBE Chatshaala (19/8/21)
We started discussion on Chai & Why session.
✓What is Chai & Why?
✓ Introduction of Chai & Why.
✓How to introduce all model systems.

So we started discussion on C.elegans & Phyllanthus.

In C.elegans model organism - we discussed how to connect Round worm in stomach with Nobel prize winning work & with Isolating of C.elegans on potato slices.

In Phyllanthus model organism - we discussed about how to connect Nobel prize winning work in fruit flies with Continuous light & dark experiment in Phyllanthus. also leaf angle titration connect with acid base titration.
Also highlighted on Phyllanthus niruri controversy.
[2:15 AM, 8/20/2021] Shraddha Sonavane CUBE: Today’s discussion was on 4 different plants -
1.Mimosa
2. Phyllanthus
3.Cardamine
4.Mango mapping

In Phyllanthus we discussed about
@Lakshmy PJ CUBE’s story of Phyllanthus-
Initially @Lakshmy PJ CUBE was working on Mimosa plants for Circadian rhythm study.she also done seed germination study in Mimosa plant.
Then she working on Phyllanthus for Circadian rhythm study, Identification of Phyllanthus species,leaf angle titration.
Collaborators- @P. Chitralekha Cube JNU mam @Nidhi @Anushka Datta @ Anulekha @Shraddha

Then we discussed on my (Shraddha) story of Phyllanthus. I found some plants in plants pot at home.
I got interested because of Circadian rhythm study was in my MSc syllabus & because of observations done by @Harshad sir,in CUBE HBCSE.
[2:15 AM, 8/20/2021] Shraddha Sonavane CUBE: Great observations done by Susanta Tanti Sir & their students while returning to Assam from Mumbai by train.
They were observed how many Mango trees was flowering & then they mapped it.

Then we discussed on Cardamine plant-

• Lifecycle of Psyche butterflies on Cardamine plant were observed in G6 lab HBCSE.

• how many days required to get first flower buds?

• after how many Compound leaves first flower buds arose?

• why Cardamine having compound leaves & Arabidopsis having simple leaves?

Which genes responsible for leaf diversity in both?

•how do gene expressed?so we compare Cardamine- plant model organism with Moina

While understanding gene expression questions was arise-
1.what is a gene?
2.where it’s located inside the cell?
3.how gene expressed?
4.why Chromosomes are important in cell?
5.what is Chromosomes & Chromatin?
@Seethalakshmy CUBE @Aswathy Suresh CUBE @Aditiya Joshi CUBE @Lakshmy PJ CUBE @Aastha Ahuja Cube @Mandar
It’s a caradamine? @Lakshmy PJ CUBE @Drishtant Cube Moina @Arunan HBSCE sir @Aditiya Joshi CUBE and others
How to identify caradamine ?
I think its oxalis… M not sure. But I thik its oxalis… This also grows as a weed in lawn, backyards, pots, gardens… @Janhvi Deshmukh
Yes I see in my area near the tulsi @Aditiya Joshi CUBE

Oxalis
oxalis
Ohh @P. Chitralekha Cube JNU mam thanks mam
[10:13 AM, 8/20/2021] Arunan HBSCE: What is the question? @Shraddha Sonavane CUBE
[10:17 AM, 8/20/2021] Arunan HBSCE: Don’t you think photos of some of your ants which you call as Black Crazy Ants in which the abdomen of the ants show prominent light band? @Seethalakshmy CUBE
Do you have any current data of Mosquitoes of Mumbai or Navimumbai? @Abhijeet Singh
What is your interpretation @Batul Pipewala Kolkata when compared with the experience of @Aswathy Suresh CUBE
One of the nematodes is much larger than the other. Any explanation? @Batul Pipewala Kolkata
Wt is the name of this nematode?
Are you convinced these are nematodes? @Ankit Yadav Xms Work Shop Cube Patkar College
Which ants are these? @Seethalakshmy CUBE please start describing from here.
What is the summary, so far, on your CUBE HomeLab for Fruity Model system? @Yash Sheregare CUBE
Yes sir. They hv all features of nematodes
What is the summary, so far, on your CUBE HomeLab for Fruity Model system? @Sidhy Kerala Drosophila
“here” means where? Please describe. What is your interpretation? @Seethalakshmy CUBE

Which Ants are these?@Seethalakshmy CUBE
Don’t you think the ants in your fomicarium looks different from these? @Seethalakshmy CUBE
Look, how this video maker use a reference scale, so well.
Great! @Seethalakshmy CUBE How many formicarium do you have in your CUBE HomeLab Pazhuvil? @Seethalakshmy CUBE
@Sidhy Kerala Drosophila @Aswathy Suresh CUBE
Maintaining log book is one of the essential part in research but CUBE always does simple things.

Why not to give update on your single line cultures and this update will be archive in this context 2 Curriculum group.

A very simple way to maintain a log book: oopps😇 e-log book (send some good photos and videos along with minimum essential write up) by sending daily update on CUBE WHAT’S APP GROUPS

@Arunan HBSCE @Batul Pipewala Kolkata @Saida Sayyed @Aditiya Joshi CUBE what do other say?
Please list those features @Ankit Yadav Xms Work Shop Cube Patkar College, so that others too will get a clear idea on what are the features of Nematodes.

Colonies on potato slices after 48hrs of spreading fermented coconut water from open bottle. Marked blue are colonies similar to that of the ones we have observed on potato slice by @Arunan HBSCE . Marked red are the cottony growth patches. Marked yellow are shiny slimy growth patches. Other white circular colonies can also be seen… 20/8/2021. Kala Talav, Kalyan west. @KIRAN CUBE @Arunan HBSCE @Aswathy Suresh CUBE @Lakshmy PJ CUBE @Batul Pipewala Kolkata @Saida Sayyed
Great!
Which colony is this?
Is it colony of yeast or fungus or any others ?
How can identify?
@Aditiya Joshi CUBE
Congrats for this summary of yesterday’s Chatshaala discussion @Shraddha Sonavane CUBE

Please write a short summary of Cardamine and Psyche butterfly interaction study that you started at CUBE Lab HBBCSE, while working on Cardamine Model system in January 2020 @Shraddha Sonavane CUBE
Control set up please, to compare @Aditiya Joshi CUBE
Hope, you had set up Control. @Aditiya Joshi CUBE

Colonies appear after 48 hours of spreading fermented coconut water from closed bottle… Various colonies can be seen… Marked blue are similar colonies we have seen got by @Arunan HBSCE . We can also see multiple small circular cream color colonies. Some white colonies. And some mucoid or slimy colonies. 20/8/2021. Kala talav, Kalyan west. @KIRAN CUBE @Arunan HBSCE @Seethalakshmy CUBE @Aswathy Suresh CUBE @Lakshmy PJ CUBE @Batul Pipewala Kolkata @Saida Sayyed

Control slices got colonies too!!! They were not inoculated with the fermented coconut water. But growth can be seen… 20/8/2021. Kala Talav, Kalyan west.
[11:36 AM, 8/20/2021] Aditiya Joshi CUBE: The setup has turned smelly… The smell similar to spoilt bread/pav.
[11:37 AM, 8/20/2021] Aditiya Joshi CUBE: There were some fruitflies on the box. A small fly must have manages to go inside… As I opened the box, it flew away…

Observation of slices after 24 hours of inoculation with fermented coconut water from open bottle. We can see some growth or colonies seen. 19/8/2021. Kala Talav, Kalyan west.

Observation after 24 hours from inoculation with fermented coconut water from closed bottle. Some colonies or growth can be seen… 19/8/2021. Kala Talav, Kalyan west.

Control slices after 24 hrs. 19/8/2021. Kala Talav, Kalyan west.
[12:58 PM, 8/20/2021] Aditiya Joshi CUBE: No ma’am. It was not aur tight. A fly managed to enter it…
[12:59 PM, 8/20/2021] Aditiya Joshi CUBE: I kept control slices on open too… But within an hour, they were gone… I think some crows or rats might have taken away or eaten up
[1:27 PM, 8/20/2021] Aditiya Joshi CUBE: Possible goof ups n sources of errors…

1. The box and slices were kept open all the time while spreading the fermented coconut water. Spreading wad done very near or next to bottles kept.
2. No test slices were kept on open.
3. Control slices kept open were lost.
4. Flies were sitting at mouth and inside of open bottle. Also they were sitting on box, lid and one also managed to enter inside.
   5.Though I have washed hands, knife, lid n box, there can be some microbes from these n air n by flies that might have settled on control slices.
5. I also opened it after 24hrs for clicking photos.
   No sir, currently I don’t have any data!
   I think we can start catching mosquito in our locality!
   In collaboration with @Hina Mudgal Kanpur CUBE Ma’am @Seethalakshmy CUBE and others!
   Hi All looking for collaboration on nematode study in the home lab, can you set up some raw potato slices with 4dtops of milk and soil to isolate nematodes and let them multiply on the potato.please reply you can ask questions if any
   Okay ma’am I’d love to collaborate
   what is your plan
   [1:41 PM, 8/20/2021] Ishita Assam: Plan of work

• collect soil sample
• potato slices to serve as medium
• control and sample
• 4 drop of milk at the center to attract the nematodes
  [1:42 PM, 8/20/2021] Ishita Assam: Ma’am when can I see the nematodes in the potato slice
  What about water to keep set up moist?
  From where will you collect soil sample?
  Yes ma’am the soil sample will be damp and also I’d have to keep the potato slice moistened using a tissue paper at the bottom
  After 24 to 48hrs of set up you should be able to see a small microbial pool on the potato slice and at the edges of this some worm like organisms 1 to 2mm in size moving
  [1:44 PM, 8/20/2021] Ishita Assam: I’d have to collect from an area where there’s a lot of trees and where leaves fall and rot
  [1:44 PM, 8/20/2021] Ishita Assam: Should I also cover it in case sone other insect lays in it?
  Since C elegans has not been found in rotting leaves but has been found in rotting fruits or vegetables soil from such a place is advisable
  Rotting fruits and vegetables, where can I get such soil then ma’am
  [1:46 PM, 8/20/2021] Batul Pipewala Kolkata: Yes but not airtight otherwise a lot of fungus grows.Insect larvae are inevitable but nothing to worry they do not harm the nematodes as I have seen in my set ups
  [1:48 PM, 8/20/2021] Batul Pipewala Kolkata: From under any Fruiting tree like jamun for example
  Okay ma’am
  Other nematodes will definitely be present in soil from other places@@Ishita Assam
  [1:49 PM, 8/20/2021] Ishita Assam: I’ll update when I start this
  [1:50 PM, 8/20/2021] Ishita Assam: Okay ma’am I will try and collect such but if I don’t I would continue with normal soil from garden and all
  Why not we do a search for to see any colony morphology resemblance? @Aditiya Joshi CUBE
  I’ll try too
  Why not? “Ideally”, won’t we expect a sample of microbes from the atmosphere or knife used for slicing potato etc.? @Aditiya Joshi CUBE
  what is your plan?
  Can’t it be the young ones! Any reference? @Batul Pipewala Kolkata
  Was the photo taken intentionally not to have light on the full slices? @Aditiya Joshi CUBE
  Yes sir. From knife, air, my hands
  Control seems to show clear colonies. Any interpretation?
  No sir. The shadow is of walls of box. N in flash light colonies were not seen much. It was shining. But yes, its opportunity for me to learn how to take better pictures n focus.
  [2:01 PM, 8/20/2021] Arunan HBSCE: Let’s start! Don’t you think this rainy season may have resulted in a surge in mosquito breeding places and mosquitoes? @Abhijeet Singh
  [2:03 PM, 8/20/2021] Arunan HBSCE: What is your guess? @Ishita Assam instead of we waiting for instructions! @Ishita Assam
  [2:05 PM, 8/20/2021] Arunan HBSCE: Can’t we challenge this? @Ishita Assam
  [2:05 PM, 8/20/2021] Arunan HBSCE: What if you do not cover it? @Ishita Assam
  [2:07 PM, 8/20/2021] Arunan HBSCE: How about designing an experiment to challenge this? @Ishita Assam
  [2:09 PM, 8/20/2021] Arunan HBSCE: Only trees? Why not under fruiting Phyllanthus plants and several others? @Ishita Assam @Batul Pipewala Kolkata
  Any clear references?
  I can collect soil samples from non fruiting area and an area where fruits fall and rot but then the thing is I won’t be sure if I’ll get C.elegans or not
  Why not post the full Design now itself, after stating the objective?@Ishita Assam
  Sir last time I got larvae in my potato slice
  Could not follow y you wont be sure if it is C elegans?Is it because you need a microscope to see their features@@Ishita Assam ?
  [2:13 PM, 8/20/2021] Arunan HBSCE: Why not do both, with a clear design starting from now? @Ishita Assam @Batul Pipewala Kolkata
  [2:13 PM, 8/20/2021] Arunan HBSCE: Design, please @Aditiya Joshi CUBE
  Yes sir I can design an experiment
  [2:16 PM, 8/20/2021] Arunan HBSCE: Please post those evidences @Ishita Assam Please give a summary and state where you got stuck?
  How are you going troubleshoot, this time? @Ishita Assam
  [2:17 PM, 8/20/2021] Arunan HBSCE: Let’s start! @Ishita Assam
  Yes sir
  What could be your research question in soil Nematodes? @Ishita Assam
  On 20th August 1897, Sir Ronald Ross discovered that Anopheles mosquitoes were responsible for the transmission of the malarial parasite. To mark this breakthrough discovery in the fight against malaria, each year we celebrate 20th August as
  World Mosquito Day
  @ hina allen cube kanpur.
  
  

MOSQUITO prevention and control. Check out for MOSQUITO breeding sites to eradicate this vector from spreading diseases like , DENGUE, malaria, chikungunya and more . @ hina, rahil , samiksha, utkarsh, vyom .

VIDEO SHARED BY BATUL AT 4.32PM
BREAKING NEWS The pharynx structure of my soil nematode looks v much like Caenorhabditis elegans from the reference given below.looking for your comments @⁨Dr Arunan⁩ @⁨Anshu Kadam⁩ @⁨Aditya Joshi⁩ @⁨Ishita⁩ @⁨Shahin🍁⁩ @⁨Akanksha⁩ video from Homelab Kolkata BatulP 20/8/21

Reference
https://www.wormatlas.org/albertson_1976/Albertson_1976.html
@Arunan HBSCE @Anshu Kadam (Nematodes) @Aditiya Joshi CUBE @ @Shahin​:maple_leaf:

VIDEO SHARED BY BATUL AT 4.45PM
As given in the reference a is the buccal cavity, b is the pro corpus, c is the meta corpus and d is the terminal bulb.video taken under 45X of compound microscope. Cube Homelab Kolkata BatulP 20/8/21
MOSQUITO prevention and control. @ cube kanpur
Presentation on cardamine made by @Priti Kanade in 2017 for National Meet!
@Arunan HBSCE @KIRAN CUBE @Saida Sayyed @Shraddha Sonavane CUBE @Seethalakshmy CUBE @Lydia cUBe Drosphila @Aswathy Suresh CUBE @Batul Pipewala Kolkata

S4E150 CUBE chatShaala: Cardamine: context to understand Gene expression and KNOXX gene

CUBE ROOM LINK Please click on the CUBE (BLUE) to join the CUBE ChatShaala Timing:5.30 pm to 9.30 pm Kindly add your place/affiliation along with your name while joining. Please click on the below given link if you face any issue in joining the webinar, i hope this would help in resolving any issue in joining. Thanks Looking forward to your participation

Kindly click on the above👆🏻 given link for the summary of last discussion.

Invitation to join today’s CUBE chatShaala,
Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in all CUBE what’s app groups and on metastudio after the session

Here’s the direct link of the CUBE webinar: https://webinar.hbcse.tifr.res.in/b/kir-hye-cn4
Note: we have changed the link to avoid technical error

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
SLTT-J
Single line culture of fruit flies.
Started on 16/08/2021,11:40 AM. Date of video: 19/08/2021
TRSV medium - 15/08/2021, 11:00 PM

Expectation:
Pupae on fourth or fifth day.
New flies on 8 th day.

Observation on 19/08/2021, 3:57 PM
~15 larvae
~25 eggs
20/08/2021, 12:18 PM ~15 larvae , ~25 eggs
Location : Thanniam, Thrissur, Kerala
Sidhy.P.P, CUBE S N College , Nattika.
SLTT-H
Single line culture of fruit flies.
Started on 16/08/2021, 11:40 AM , Date of video: 19/08/2021
TRSV medium - 15/08/2021, 11:00 PM

Expectation:
Pupae on fourth or fifth day.
New flies will emerge on 8 th day.

Observation on 19/08/2021, 3:56 PM
~ 30 larvae
~ 20 eggs
20/08/2021,12:20 PM : ~ 40 larvae , ~20 eggs
Location : Thanniam, Thrissur, Kerala
Sidhy.P.P, CUBE S N College , Nattika.

VIDEOS SHARED BY SIDHY AT 5.16PM
SLTT-I
Single line culture of fruit flies.
Started on 16/08/2021, 11:40 AM. Date of video:19/08/2021
TRSV medium - 15/08/2021, 11:00 PM

Expectation:
Pupae on fourth or fifth day.
New flies on 8 th day.

Observation on 19/08/2021, 3:56 PM
~ 15 larvae
~ 20 eggs
20/08/2021, 12:18 PM ~ ~25 larvae , ~20 eggs
Location : Thanniam, Thrissur, Kerala
Sidhy.P.P, CUBE S N College , Nattika.
MOSQUITO prevalence in water accumulation, Rainy season are hot spots for them to breed . Check out for them. Spread awareness of this vector. Kill it before it spreads another epidemic . @ hina cube kanpur , dated 20. O8.21
A fruitfly on onion piece… 8:19 pm. Kala Talav, Kalyan west. 20/8/2021
Pic by salman mustafa, location swaroop nagar. Time 7.00pm
20.08.21

• Needs better focus .
  @ hina Allen .

What is RER test? Is it possible to make alternatives to perform RER at home?

How can we culture Earthworms easily using simple things at home?

RER Test Experiment : Making a design of experiment how to perform in a simple way that every ones can do it in their home labs!

Which kind of nutrition will get earthworms culturing by newspaper?

What are the components presents in Newspaper?

Cellulose are carbohydrates!

What is the difference between starch & cellulose?

Beta Glucose & Alpha Glucose Differences?

@Arunan HBSCE sir @Aditiya Joshi CUBE @Abhijeet Singh @Seethalakshmy CUBEObjective was to observed after how many Compound leaves first flower buds arose.
519 th day of CUBE ChatShaala: 20th August 2021.

https://www.google.com/maps/d/edit?mid=130gpg9gUtd61uh2e-7vDbctkkfTpu4xq&usp=sharing

18 CUBists from 18 Centres had joined the webinar.

INDIA

Assam
Ishita Sonowal: Jorhat

Delhi
Aastha Ahuja: ANDC

Haryana
Aashi: Panipat

Kerala
Aswathy Suresh: Thriprayar
Seethalakshmi CR: Pazhuvil, Thrissur
Siddhy: Thanniam Thrissur

Maharashtra
Abhijeet Singh: Ulwe, Navi Mumbai
Aditya Joshi: Kalyan
Arunan MC: Breach Candy, South Mumbai

Uttar Pradesh
Kiran Yadav: Moradabad
Divyanshi Chauhan: Noida
Ravleen Kaur: Bijnore

Uttrakhand
Sakshi

West Bengal
Batul Pipewala: Kolkata

Meena
Medha
Prachi Pant
Deeksha Pandey

Note: Cubist(s) who had joined today but their name isn’t included in the list, kindly replied to this message[12:26 AM, 8/21/2021] Shraddha Sonavane CUBE: Cardamine- Psyche butterflies interaction

Cardamine is new host plant for Psyche butterflies.

While working on Cardamine model organism in January 2020, initially I was observed one Caterpillar on Cardamine plant​:seedling: in 2nd right side patch of Cardamine forest in front of HBCSE main building.
I was curious about caterpillar so I was collected caterpillar with same Cardamine plant on which caterpillar found.
I kept same plant in container having some water so all roots dipped inside Water.
Then,This container & some more Cardamine plants were added in another container. both containers were kept in glass tank.
One Open side of glass tank was tightly covered with net & rubber band.

After 4-5 days I observed Caterpillar was turned into green pupa.In between 4-5 days, I observed that Caterpillar was eaten most of leaves & fecal matter was there.

After 5-6 days, new adult white butterfly with prominent black spot on upper wing was emerged.

Because of their appearance I could easily found that their name - Oriental Psyche (Leptosia nina nina)
[12:27 AM, 8/21/2021] Shraddha Sonavane CUBE: Because of more observations I could found one Egg & 6 more Caterpillars on Cardamine plants

The Egg was cylindrical shape & white in colour on terminal leaflet in upright standing manner.

Again Similar set up were made to study life cycle of newly found Caterpillars.

In these set up I could observed 2 different pupa colour- 1 green colour &
2. Yellowish brown/Creamish colour.

But when adult butterflies emerged, I could observe
2 different eyes👀 colour- 1.Brown colour
2.green colour
Summary of 519th day of Cube Chatshaala*
We started the discussion with what I(Abhijeet) have plan for the Chai and Why? Programme.
So I’ve worked on Model Organisms like Mosquito, Earthworm, and Snails when I was active in cube in my secondary school (7th to 9th std)
So today we discussed about Earthworm.

How can we culture and maintain Earthworms in our Homelab?
So culturing earthworms at home would be very easy! We don’t need high-tech laboratory stuff. We just need a plastic cup, paper, earthworm and waste cloth

1. Take cup and pinch holes at bottom with the help of a pin.
2. Make pieces of paper, and fill 1/3rd of the cup.
3. Make that paper inside cup moist with water
4. Put earthworm inside cup.
5. Cover the cup with cloth and rubber band.
6. keep the cup(s) inside a tray with newspaper wetted with enough water .What’s special in Earthworms?
   Humans have Spinal cord which helps in making co-ordination between different body parts in each activity that we do throughout. If due to some reason the spinal cord is injured we get paralyzed and the co-ordination is lost.

Earthworms have Ventral Nerve Cord(VNC) with same function as Spinal cord in humans i.e maintaining coordination in body. But if VNC of earthworms get injured it gets regenerated

So can we study how this regeneration happens in earthworms but not in humans!!!

RAPID ESCAPE RESPONSE TEST (RER) was designed to see how in how much time this regeneration of VNC occurs and also for selecting earthworms with good co-ordination in their body.

How is RER test performed!?

1. Make salt solution of a particular concentration.
2. put some 2-5 ml of that in test-tube(experiment)
   3)take another test-tube fill with 2-5ml of water(control)
3. add one earthworm at a time in both and watch their response within the 2 min time
4. note the observation.
5. Remove the earthworm from soln and wash is very nicely and keep the back in culture
   Expectations:-
6. The earthworm should immediately response towards the salt solution and escape from it.

Inference:-

1. As the salt solution is harmful for earthworms they should escape from it showing excellent co-ordination. And earthworm in water(control) would not show any response towards water.
2. Earthworm which showed the coordination and came out of salt solution can be selected for studying.
   After RER test we can use the earthworm that passed the test for studying the time it takes for them to regenerate their VNC.

On the ventral part we give a lesion(enough that we can say that VNC is lesioned).
After giving the lesion perform the RER test to confirm that earthworm’s body co-ordination is lost.
Expectations:- The earthworm should not be able to escape the salt solution.

Continue doing RER test with 2 days interval till the VNC is regenerated and the co-ordination is gained again.

@Aswathy Suresh CUBE can you please comment on how can we perform RER in our homelabs!
@Aditiya Joshi CUBE @Seethalakshmy CUBE please comment on the nutrition that can be derived by earthworm by eating paper in cup cultures!!!When I (Shraddha) & @KIRAN CUBE mam did search on Leptosia nina - Wikipedia & https://www.ifoundbutterflies.org/sp/811/Leptosia-nina/early-stages
for host plants of Psyche butterflies, we found that Cleome,Capparis are host plants but not Cardamine plants

[KiranKalakotiR (Kiran Kalakoti)]

21st August 2021, 9.30pm to 22nd August 2021, 9.30pm
But I got complete life cycle of Psyche butterflies on Cardamine plants.

So we did survey in whole HBCSE campus for Cleome or Capparis & for Cardamine plants
& we get only 3 Cleome plants in whole Campus (at besides G6 lab, near BARC Compound ), whereas we observed huge Cardamine forest (more than 1000 plants) in whole Campus. (Mostly at sorrounding to main HBCSE building)

So it was like ecological survey of 3 different plants in HBCSE campus.

So, Questions came in mind-

✓Cleome & Capparis are belongs to Cleomaceae & Capparaceae family resp.
✓Cardamine belongs to Brassicaceae/ Cruciferae family.
1.why female Psyche butterflies laid egg on Cardamine plants?
✓we get an answer that all 3 families are different but they having common origin meaning they are belonging to same Order - Brassicales

The characteristics of Brassicales order is they are rich source of secondary metabolites i.e.glucosinolates

Hence in all 3 plant families members, Glucosinolates is present.

because of more number of Cardamine plants compare to Cleome/Capparis plants in a Campus so for survive female Psyche butterflies choose Cardamine plants for egg laying.

All observations done in HBCSE campus.
Date- during Jan - Feb 2020.

Collaborators during observations- @KIRAN CUBE mam @Yash Sheregare CUBE @Shivam CUBE NEMATODE
Collaborators in India- @drishya @arunima & all others…

Size of the ant. By using reference sclae. Size: 2.3-3mm.
Time:6:56 pm.
Location : Pazhuvil.
Date:20/8/21.
Data collector : Seethalakshmi. C.R.
Yes sir . They have

VIDEO SHARED BY SEETHALAKSHMI AT 1.43PM
@Arunan HBSCE sir
They have the white bands
Location: Pazhuvil
Date: 20/8/21
Time:7:09 pm
Data collector : Seethalakshmi
Wonderful explanation @Shraddha Sonavane CUBE
Glucosinolates acts like antibiotics so it might help to protect egg from any fungal or bacterial infection
Update on Ant Model system
Location :Pazhuvil
Time:5:27pm
Date :20/8/21 , Data collector : Seethalakshmi
The size of leaves of Cleome and Capparis can also be one of the factor Shraddha
Plant kept in LED for one month and shifted to normal conditions,shows delayed opening of leaves
Plant s under normal conditions
[8:40 AM, 8/21/2021] Shraddha Sonavane CUBE: Thank you @Ankit Yadav Xms Work Shop Cube Patkar College

Yes. Glucosinolates help in identification of host plants & as you said it also help in protection of next generations.

It means female butterflies not only select food for next generations but also protect themselves from microbial infections and other predators attacks.

Glucosinolates are slightly bitter in taste so, many organism not prefer glucosinolates containg food.
this is winning situation​:partying_face: for Psyche butterflies.

Because less competition for food and When Caterpillar eat these glucosinolates containg food it might chances that in their guts some amount of glucosinolates present so probably less chance of predators attacks.
[8:45 AM, 8/21/2021] Shraddha Sonavane CUBE: Sorry mam I didn’t get.
But You mean size of leaves of Cleome & Capparis is larger than Cardamine is it?
@Sunita Chahar CUBE mam
[8:53 AM, 8/21/2021] Shraddha Sonavane CUBE: Congratulations @Sunita Chahar CUBE mam
plant are still surviving in stress conditions for more than one month.
[8:53 AM, 8/21/2021] Shraddha Sonavane CUBE: How many minutes/hours of delayed?

What’s leaves opening time in Control plants and in shifted plant?
I could see some white cottony structure on branches. What are they?

Is it mealy bug attack or something else?
[9:17 AM, 8/21/2021] Abhijeet Singh: Okay, this was new for me!!!
Does butterfly actually select the plant to lay eggs?
@Shraddha Sonavane CUBE @KIRAN CUBE
[9:19 AM, 8/21/2021] Abhijeet Singh: If you have photos please send it @Shraddha Sonavane CUBE !!!
@Hina Mudgal Kanpur CUBE​:innocent: Looking for updates on Mosquito studies from CUBE Kanpur to inspire others.
Not convincing evidence. @Batul Pipewala Kolkata Please give references from Wormbook showing how O.tipulae looks. @Anshu Kadam (Nematodes) please collaborate.
So @Sunita Chahar CUBE Ma’am before keeping the plant in LED what was the pattern in opening of leaves and what’s the delay that you are referring to after one Month!?

That’s something very interesting @Sunita Chahar CUBE Ma’am. Please let us know more about this with context!!
Excellent collections from CUBE Adarsha Vidylaya collection @Abhijeet Singh No names of Collaborators? @Abhijeet Singh
GREAT! @Sidhy Kerala Drosophila
Please post daily updates and make comments on collaborators’ posts, to move forward collectively. @Sidhy Kerala Drosophila @Aswathy Suresh CUBE @Yash Sheregare CUBE @Aastha Ahuja Cube and others
CONGRATS! @Sidhy Kerala Drosophila
Yes. female butterflies sense plant with help of Chemoreceptors. These Chemoreceptors are present on their legs
[9:39 AM, 8/21/2021] Arunan HBSCE: Where exactly this was located sp that you can warn others to look for such habitat spots of Mosquito breeding? @Hina Mudgal Kanpur CUBE Please take initiative to get more collaborators in this nationally important CUBE Public Health Research.
@Hina Mudgal Kanpur CUBE @Abhijeet Singh Right time to prevent mosquito breeding across the country.
[9:46 AM, 8/21/2021] Arunan HBSCE: What is the objective of Cubist Salman Mustafa and his collaborators? @Hina Mudgal Kanpur CUBE
What is your comment? @Abhijeet Singh from your school cubists experience?
[9:47 AM, 8/21/2021] Arunan HBSCE: Comments please @Ishita Assam @Abhijeet Singh @Sachin Pradhan CUBE
[9:49 AM, 8/21/2021] Arunan HBSCE: GREAT! @Shraddha Sonavane CUBE Who were your collaborators in Cardamine- Psyche butterfly interaction research? @Shraddha Sonavane CUBE
[9:50 AM, 8/21/2021] Arunan HBSCE: Please list the names and centers of your collaborators @Shraddha Sonavane CUBE
[9:52 AM, 8/21/2021] Arunan HBSCE: Please make comments @Ishita Assam @Aswathy
@Aswathy Suresh CUBE @Seethalakshmy CUBE and others
[9:55 AM, 8/21/2021] Arunan HBSCE: Have you found locations near your residence where we can expect to find Earthworms, for developing model systems for Spinal cord regeneration studies? @Aswathy Suresh CUBE @Sidhy Kerala Drosophila @Ishita Assam @Abhijeet Singh @Seethalakshmy CUBE
[9:57 AM, 8/21/2021] Arunan HBSCE: Can cellulose in paper alone be sufficient nutrient source, for maintaining Earthworm culture? @Abhijeet Singh @Ishita Assam @Aswathy Suresh CUBE @Sidhy Kerala Drosophila
[10:08 AM, 8/21/2021] Arunan HBSCE: Do the Earthworms eat paper and defecate in the same cup? Would it become like Vermicompost, in the culture cups? @Abhijeet Singh @Aditiya Joshi CUBE @Ishita Assam @Sachin Pradhan CUBE
[10:11 AM, 8/21/2021] Arunan HBSCE: Photos please of Cardamine & Cleome sites as evidence @Shraddha Sonavane CUBE
Great write up @Shraddha Sonavane CUBE
I think it will help to get all life cycle of Psyche butterflies on Cardamine plant *Cardamine* plant: New host plant of Psyche butterflies(Leptosia nina)
What are Glucosinolates? @Shraddha Sonavane CUBE @KIRAN CUBE
Can you comment on the achievement by the addition of a single step in the method while posting photos of ants? @Seethalakshmy CUBE
Do you still think these are Crazy Black Ants? Which Genus and species? @Seethalakshmy CUBE
Collaborators - @KIRAN CUBE mam @Yash Sheregare CUBE @Shivam CUBE NEMATODE
[10:18 AM, 8/21/2021] Arunan HBSCE: Any reference to convince all that these are the Black Crazy Ants? @Seethalakshmy CUBE
How do workers differ from male ants of this species? @Seethalakshmy CUBE
[10:19 AM, 8/21/2021] Arunan HBSCE: Any reference? @Ankit Yadav Xms Work Shop Cube Patkar College
[10:20 AM, 8/21/2021] Arunan HBSCE: Please mention your objectives with each such post for others to take interestin your work @Seethalakshmy CUBE
[10:21 AM, 8/21/2021] Arunan HBSCE: Please clarify @Sunita Chahar CUBE What is your suggestion?
[10:23 AM, 8/21/2021] Arunan HBSCE: Please give details for others to understand and to get inspired and to make comments. @Sunita Chahar CUBE
[10:24 AM, 8/21/2021] Arunan HBSCE: Time of opening and closing please, of each.
And please state the objective of this work.@Sunita Chahar CUBE
Collaborators In India- Drishya from SN college Nattika , Arunima from Kozhikode, Kerala ,@batul mam from Chandani, Kolkatta ,West Bengal.
Wormbook comparision of O tipulae is a drawing whereas I have given a pic from wormatlas showing a light micrograph and it is matching please comment @Arunan HBSCE
[10:25 AM, 8/21/2021] Arunan HBSCE: Reference please @Shraddha Sonavane CUBE
[10:27 AM, 8/21/2021] Arunan HBSCE: Yes, kindly articulate your comments clearly, so that all can understand and thereby extend the conversation @Sunita Chahar CUBE
[10:29 AM, 8/21/2021] Arunan HBSCE: What exactly did you understand from the posts of @Sunita Chahar CUBE on Phyllanthus @Shraddha Sonavane CUBE
Please summarise what you understood. Let’s have clearer discussion. @Shraddha Sonavane CUBE your participation is very effective.
[10:31 AM, 8/21/2021] Arunan HBSCE: Reference please @Shraddha Sonavane CUBE
[10:34 AM, 8/21/2021] Arunan HBSCE: Excellent! @Shraddha Sonavane CUBE
Don’t you think we should further confirm from other centers, though collaborators? @Shraddha Sonavane CUBE
[10:35 AM, 8/21/2021] Arunan HBSCE: Please mention their contribution in this work @Shraddha Sonavane CUBE
[10:36 AM, 8/21/2021] Arunan HBSCE: Pictures please repost with references for all to make comments @Batul Pipewala Kolkata
Referencehttps://www.wormatlas.org/albertson_1976/Albertson_1976.html

VIDEO SHARED BY BATUL AT 11.28AM
As given in the reference a is the buccal cavity, b is the pro corpus, c is the meta corpus and d is the terminal bulb.video taken under 45X of compound microscope. Cube Homelab Kolkata BatulP 20/8/21
BREAKING NEWS The pharynx structure of my soil nematode looks v much like Caenorhabditis elegans from the reference given above.please comment @Anshu Kadam (Nematodes) @ @Arunan HBSCE @Ishita Assam @Shahin🍁 video from Homelab Kolkata BatulP 20/8/21h
[11:35 AM, 8/21/2021] Lakshmy PJ CUBE: This cardamine plants brought from plant nursary. @KIRAN CUBE
I dont know i think it may be cardamine hirsuta or cardamine flexuosa
[11:35 AM, 8/21/2021] Lakshmy PJ CUBE: Thrissur, Mampilly
[11:37 AM, 8/21/2021] Lakshmy PJ CUBE: I expect within 40-50days. There will be first floral bud.
[11:39 AM, 8/21/2021] Lakshmy PJ CUBE: There is gene called KNOX gene respondsible in formation of compound leaves in cardamine. @Arunan HBSCE @KIRAN CUBE @Shraddha Sonavane CUBE
There is one major difference between Starch and Cellulose.
For starch, glucose repeat units are located in the same direction, and each successive glucose unit is rotated 180 degrees in cellulose.

Cellulose is thicker than sugar, which is water-insoluble. On the other hand, the Starch is less weak than cellulose and can be dissolved in warm water.

Difference Between Starch and Cellulose - Similar polymers on BYJU’S

@Arunan HBSCE sir, @Aswathy Suresh CUBE, @Aditiya Joshi CUBE, @Abhijeet Singh
And others
[12:36 PM, 8/21/2021] Arunan HBSCE: On what basis we can say this? @Lakshmy PJ CUBE How are Cardamine hirsuta and Cardamine flexuosa simar and different from other Cardamines? @Lakshmy PJ CUBE
Don’t we have any reference on Indian Cardamine species?@Lakshmy PJ CUBE
[12:39 PM, 8/21/2021] Arunan HBSCE: How does Knox gene result in formation of compound leaves? @Lakshmy PJ CUBE Please give some relevant references and say what do you understand.
[12:40 PM, 8/21/2021] Arunan HBSCE: Can’t we get better reference than that of coaching classes? @Seethalakshmy CUBE
Let’s all learn together. @Seethalakshmy CUBE

Fig SI9. The difference between starch and cellulose packing structures

Comparison between Starch, Glycogen and Cellulose

Cellulose is a linear polymer and starch is consist of linear and branches. These arrangement do effect the properties.
The reason is due to the different types of bonding between cellulose and starch. Cellulose has beta-1,4 bonds that are not digested by our enzymes (which can digest alfa-1,4 and alfa-1,6 bonds that are present in starch and glycogen). However, some bacteria from the gastrointestinal tract of human and other animal species can use cellulose as an energy source since they are able to break the beta 1,4 bonds. Actually, that is one of the concepts of fiber (carbohydrates that are not digested by the enzymes produced by the animal, being fermented by bacteria).
The arrangement of the glucose units in starch also differs between amylose (linear structure with alfa-1,4 bonds) and amylopectin (branched structure with alfa-1,6 bonds), explaining the differences in glucose absorption between ingredients with different proportions of these two polymers.

VIDEOS SHARED BY ASWATHY AT 1.17PM
SLBK B 21
[single line 22ndsubculture bottle of B line (species).]

Update : 21 August 2021 ; 1:04pm

Date of transfer flies : 12.08.2021
Transfer no of flies : 12 Flies.
Gravid Flies : 5, Male Flies : 7

Expectations on 9th Day
21/08/2021
~60 Larvae
~60 Pupae
~40 Flies (including new flies)

Observations on 9th Day
21/08/2021
~ 20 Larvae
~ 70 Pupae
~35 Flies (including new flies)

Update : Aswathy Suresh CUBE SN College Nattika.
Home Lab : Bottukadavu - Thrissur Kerala.
SLBK E17
[single line 18thsubculture bottle of E line (species)]

Update : 21 August 2021 ; 1:12pm

Date of transfer flies : 12.08.2021
Transfer no of flies : 14 Flies.
Gravid Flies : 6, Male Flies : 8

Expectations :
21.08.2021 : 9th Day
~80 Eggs
~50 Larvae
~40 Pupae

Observation : 9th Day
21/08/2021
No of Eggs : 50
No of Larvae : 20
No of Pupae : 20
No of Flies : ~10 (Including newly emerged flies).
Dead Flies : 5

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
We baked bread today. This cloth was used to cover the dough while kept for rising. Smell of Yeast on the fermenting dough may have attracted Fruitflies to this piece of cloth. 20 August 2021 CUBE HomeLab Sophia, Bhulabhai Desai Road, Mumbai Photo: mcarunan
SLBK A 22
[single line 23rdsubculture bottle of A line (species).]

Update : 21 August 2021 ;1:21pm

Date of transfer flies : 12.08.2021
Transfer no of flies : 1 (Gravid)

Expectations on 9th Day
21/08/2021
~50 Eggs
~20 Larvae
~10 Pupae
~40 Flies (including new flies)

Observations on 9th Day
21/08/2021
~20 Eggs
~ 3 Larvae
~ 0 Pupae
1 Fly (Transferred)

Update : Aswathy Suresh CUBE SN College Nattika.
Home Lab : Bottukadavu - Thrissur Kerala.
SLBK E15

Update : Fruit Flies
subculture started date : 02.07.2021

Transfer Flies : 15 Flies
Gravid Flies : 8, Male Flies : 7

Expectations :
21.08.2021 : 50 Days old culture bottle
~200 Eggs
~60 Larvae
~100 Pupae

Observation : 50th Day
21/08/2021
No of Larvae : ~10
No of Pupae : ~200
No of Unhatched Pupae : ~70
No of Flies : ~10
Dead Flies : ~70+

Media solidified

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
SLBK E16

Update : Fruit Flies
subculture started date : 19.07.2021

Transfer Flies : 30 Flies
Gravid Flies : 9, Male Flies : 21

Expectations :
21.08.2021 : 34th Day
(More than one month old culture)
~350 Eggs
~80 Larvae
~160 Pupae
~100 Flies

Observation : 34th Day
21/08/2021
No of Eggs : ~150+
No of Larvae : ~30
No of Pupae : ~200+
No of Unhatched Pupae : ~40+
No of Flies : ~70+
Dead Flies : 80+

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
[1:45 PM, 8/21/2021] Arunan HBSCE: Please post separate ose up, photos showing larvae and eggs in this Bottle SLBK A22, as clear evidence. @Aswathy Suresh CUBE
[1:46 PM, 8/21/2021] Arunan HBSCE: Close* up

Most of eggs present inside the media, ~100 more eggs observed in E16 bottles. Picture taken from SLBK E16. Date of picture 2:43pm.

SLBK A22 - 1st instar larvae found inside culture bottle, picture taken on 21/08/2021: 2:53pm
Okay
Any update on this now?
Nice!
How do you make sure that there are still new flies emerging in this bottle?

What are your plans with these cultures?
[3:27 PM, 8/21/2021] Lydia cUBe Drosphila: This is not that old bottle. How long does a culture bottle survive on average?

And what could be the reason for the death of so many flies? What do you think?
[3:31 PM, 8/21/2021] Lydia cUBe Drosphila: Yes understood
Why not explain using some examples? Some relevant examples of proteins…a protein that can reverse back to the original structure and another protein that is irreversible…a comparison kind of.
By this, we’ll always remember such examples.
[3:33 PM, 8/21/2021] Lydia cUBe Drosphila: How does a non-clubbed antenna look?
Can you please send some pictures of the ants with these characteristics that you think are morphological features.
Within 5 days, 10 flies dead!
Were those flies too old when you transferred?
[3:36 PM, 8/21/2021] Lydia cUBe Drosphila: What’s the difference between these 2 kinds of ants?
[3:39 PM, 8/21/2021] Lydia cUBe Drosphila: Are those white stripes on aedes mosquitoes legs quite visible?
[3:44 PM, 8/21/2021] Lydia cUBe Drosphila: True!
Got it
I got some doubts from some of my friends… I wasn’t able to convince them completely w.r.t. organism and system…so thought of asking.
Thank you!
[3:44 PM, 8/21/2021] Lydia cUBe Drosphila: Yes exactly!
We’ll get a context to get to know more on these curriculum topics!
[3:52 PM, 8/21/2021] Lydia cUBe Drosphila: Egg yolk too contains proteins right?
So same is the case for both egg whites and egg yolk…right?
[3:55 PM, 8/21/2021] Lydia cUBe Drosphila: Would the presence of fats interfere with the protein denaturation process?
[3:59 PM, 8/21/2021] Lydia cUBe Drosphila: Yes Sir!
True!

A healthy culture bottle will give us all the stages of a fruit fly! We do expect to see eggs, larvae, pupae and adult flies!
More interested to see the larva, as an indicator that fertilized eggs hatch to larvae.
Please post larvae of SLBK A22.
How are we sure this is 1st instar larva? @Aswathy Suresh CUBE

S4E151 CUBE chatShaala: Understanding regeneration

CUBE ROOM LINK Please click on the CUBE (BLUE) to join the CUBE ChatShaala Timing:5.30 pm to 9.30 pm Kindly add your place/affiliation along with your name while joining. Please click on the below given link if you face any issue in joining the webinar, i hope this would help in resolving any issue in joining. Thanks Looking forward to your participation

Kindly click on the above👆🏻 given link for the summary of last discussion.

Invitation to join today’s CUBE chatShaala,
Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in all CUBE what’s app groups and on metastudio after the session

Here’s the direct link of the CUBE webinar: https://webinar.hbcse.tifr.res.in/b/kir-hye-cn4
Note: we have changed the link to avoid technical error

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
INVITATION TO JOIN TODAY’S CUBE CHATSHAALA

Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in CUBE what’s app groups after the session

Webinar Link :

Jitsi Meet

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
Attention!

Owing to unexpected technical issues regarding the regular Big Blue Button Platform where the CUBE Chatshaala is held everyday, we are shifting (temporarily, for today) to

Jitsi.

Jitsi Meet

In case of any issues, please let us know in the group or on personal.

VIDEOS SHARED BT BATUL AT 6.21 PM
BREAKING NEWS The pharynx structure of my soil nematode looks v much like Caenorhabditis elegans from the reference given above.please comment
As given in the reference a is the buccal cavity, b is the pro corpus, c is the meta corpus and d is the terminal bulb.video taken under 45X of compound microscope. Cube Homelab Kolkata BatulP 20/8/21

Referencehttps://www.wormatlas.org/albertson_1976/Albertson_1976.html
Wow

Summary of CUBE Chatshaala(21/8/21)

Discussed about *Circadian rythm( sleep wake cycle )
vs
Circanual rhythm.( mango fruiting )

• What is Altitude and Longitude? How can define?

• Why it is Important in Mango maping?

• Mango starts to Flowering at first in which region ( North or South)?
  As well as which month??

Others please give your inputs
@Arunan HBSCE sir, @Aswathy Suresh CUBE, @Abhijeet Singh, @Saida Sayyed
And others
[12:14 AM, 8/22/2021] Shraddha Sonavane CUBE: Yes Sir, It will be really great if @Arunima SN Nattika Cardamine @Drishya share their work on Butterflies & Cardamine interaction in their Home labs. @Arunima SN Nattika Cardamine & @Drishya can you please share your work on Butterflies & Cardamine interaction
[12:16 AM, 8/22/2021] Shraddha Sonavane CUBE: because of collaborations only, we could provide Nectar for newly emerged Psyche butterflies by providing Sugar solution with help of cotton & Vernonia plants as Nectar plant.
Summary of 520th day of Cube Chatshaala
Discussion started with @batul Ma’am’s nematodes culturing and identification.
Currently batul Ma’am culturing nematodes on potato slices. With that she is trying to identify C.elegans from her nematodes if there’s any in her culture!
Identification:-
So for identification of C.elegans she is using a photo of C.elegans from a research paper(The Pharynx of Caenorhabditis elegans Albertson 1975) and compare the morphological characteristics with the nematodes that she has cultured in her homelab!
Identification so far:-
So far she is able to compare the Anterior part(i.e. pharynx).
After that discussion went on about the speculation and casual observation that we make in our experiments and our interpretation of that! With reference to Aswathy saw Nematodes after 15days in her drosophila TRSV media which has vinegar and Batul Ma’am is not able to culture nematodes with RSV media with has Vinegar.
A very important point that came after discussion is that:-
Speculation, Casual observation is always welcomed!! but it is to be verified with evidence and experimental design with enough replicates and control

This leads us to the very fundamental idea of CUBE and ofcourse of science is:- CONSTRUCTION AND NOT INSTRUCTION
This knowledge construction can be through 2 types of collaborators:-
1)Involved in Discussion
2)Working on same model organism

Moving forward with Discussion on Circadian Rhythm and understanding it properly. A circadian rhythm or circadian cycle, is a natural, biological process that regulates and repeats roughly every 24 hours. … The term circadian comes from the Latin circa, meaning “around” (or “approximately”), and diēm, meaning “day”.(example:- opening and closing patter of leafs in phyllanthus)
Also Circannual Rhythm. The term circannual is Latin, circa meaning approximately and annual relating to one year.(example mango flowering)
Moving forward with discussing the CIRCADIAN RHYTHM with respect to stomatal opening and closing in plant’s leafs and photosynthesis process(with context to secondary school biology curriculum) .
AND
CIRCANNUAL RHYTHM with respect to mango Flowering and Fruiting pattern throughout India.

3days after inoculation of fermented water. Slices in red marked outline are inoculated with fermented coconut water from closed bottle. White cottony colonies, Greenish n blackish colonies are seen. Also, circular whitish colonies can be seen too. Also few small thread like colonies too. Marked blue are slices inoculated with fermented coconut water from open bottle. Thread like colonies are seen. Also blackish n white cottony growth are seen… Thread like colonies are seen on control slices. The color of these thread like colonies is seen to be changed. 21/8/2021. Kala Talav, Kalyan west.

There is a nasty smell… Strong smell similar to that of spoilt bread… There were some small flies too
[7:28 AM, 8/22/2021] Abhijeet Singh: How this chemoreceptors help them! @Shraddha Sonavane CUBE
Please elaborate!
[7:39 AM, 8/22/2021] Abhijeet Singh: I think sir! Because we have seen earthworm surving on paper for a complete year!
Humans cannot digest Cellulose and that us why I think we cook our vegetables and then eat!
But earthworm can digest it and survive it.
So cellulose can be the source of nutrition for earthworms to survive!
[7:39 AM, 8/22/2021] Abhijeet Singh: What do you think @Aswathy Suresh CUBE @Aditiya Joshi CUBE @Ishita Assam and others!!?
[7:44 AM, 8/22/2021] Abhijeet Singh: Yes Yes! That’s is very interesting thing sir! I would like to tell this to everyone that, in cube lab, we had a one year old cup culture. Same tissue paper was given to earthworms to feed on. The the tissue paper in the cup was eaten and again eaten and again since one year! So we can literally see that the paper had become very similar to what soil looks like!
I’ll look for photographs from collaborators if I can get I’ll post it in the group!
Koel call
Date : 21.08.2021
Time : 5:46pm
Location : Bottukadavu - Thrissur Kerala.
Data collector : Aswathy Suresh CUBE S N Nattika
How do you compare with diagrams in this reference?
@Batul Pipewala Kolkata @Anshu Kadam (Nematodes) @Saida Sayyed @Aswathy Suresh CUBE @Lydia cUBe Drosphila

Figure 4, Distinguishing features of Caenorhabditis elegans, compared to a...

What is the difference between alpha & beta 1,4 linkage? @Seethalakshmy CUBE
Any clear diagram to distinguish? @Seethalakshmy CUBE @Abhijeet Singh @Aswathy Suresh CUBE @Aastha Ahuja Cube @Raj Yashwantrao Patil CUBE @Sachin Pradhan CUBE @Ishita Assam
My plan is to transfer these flies in to new media bottles.
Also planing to harvest second instar larvae using these flies in order to perform olfactory assay experiment.
[11:11 AM, 8/22/2021] Arunan HBSCE: @Shraddha Sonavane CUBE What do you think is the work of Drishya and @Arunima SN Nattika Cardamine
[11:12 AM, 8/22/2021] Arunan HBSCE: Please mention the major contribution of each of your collaborators @Shraddha Sonavane CUBE
One healthy culture bottle survive maximum 2 months. After media become liquefied and also flies,larvae,pupae number will decrease inside after 2 months old culture bottles.
Can you tell us first about what you expected in Control slices?
Please give *your interpretation based on these Control slices, after writing subheadings. @Aditiya Joshi CUBE
Transferred flies from B20 bottles which was made on 19.07.2021 after I transferred in to B21-12.08.2021 bottle. ~20 days old flies I transferred from B20 to B21 bottle.
[11:17 AM, 8/22/2021] Arunan HBSCE: Please be clearer in your description starting with Control. @Aditiya Joshi CUBE
[11:20 AM, 8/22/2021] Arunan HBSCE: What nutrition Earthworms can derive from Cellulose? @Abhijeet Singh @Seethalakshmy CUBE @Aswathy Suresh CUBE
What about other nutrients?
[11:24 AM, 8/22/2021] Aswathy Suresh CUBE: Yes sir, This is a zoomed picture of larvae that I taken from SLBK A22. Picture is not much clear, But we can able to see the mouth hook part of this larvae is not much fully developed. 2 black sports kind thing can see at anterior portion. Spiracles is not present at the tip of body. that’s why I concluded this is first instar larvae of fruit flies
[11:32 AM, 8/22/2021] Aswathy Suresh CUBE: Yesterday discussed about Mango flowering in different parts of country based on Latitude & Longitude. Is there any significants chances we can able to see in mango flowering from lower to higher latitude (N) regions.
Also discussed about importance of latitude and longitude marking while in data collection.

[KiranKalakotiR (Kiran Kalakoti)]

22nd August 2021, 12.09pm to 23rd August 2021, 9.30pm
Guava …
Please give reference link of larval features of 1st, 2nd and 3rd instar, to say why you think this is 1st instar and hence to convince all. @Aswathy Suresh CUBE
[12:46 PM, 8/22/2021] Arunan HBSCE: Wonderful! @Prashant Kumar Sahu
Please te us the exact Location, Date and Name of Photographer along with each photo. Please repost with essential description @Prashant Kumar Sahu
[12:48 PM, 8/22/2021] Arunan HBSCE: Please start giving your Hypothesis on Mango fruiting with respect to a. Latitudes & b. Longitudes in India @Aswathy Suresh CUBE @Batul Pipewala Kolkata @Saida Sayyed @Abhijeet Singh
[12:48 PM, 8/22/2021] Arunan HBSCE: @Seethalakshmy CUBE
[12:51 PM, 8/22/2021] Arunan HBSCE: Please write about your prediction on Mango flowering in Kanyakumari and Thiruvananthapuram, as discussed yesterday, based on previous experience of Cubists? @Aswathy Suresh CUBE @Abhijeet Singh @Seethalakshmy CUBE
How do Mango trees respond in the 3rd week of August? CUBE HomeLab Sophia, Bhulabhai Desai Road, South Mumbai, Latitude19°N, Longitude79°E 20 August 2020 Photo: mcarunan
[1:39 PM, 8/22/2021] Lydia cUBe Drosphila: Interesting!
What do you think of these observations that you got? Why is there a difference?
One more thing…after inoculating these potato slices, where did you keep them? And for how long?
[1:43 PM, 8/22/2021] Lydia cUBe Drosphila: In what all is cellulose present other than paper?
[1:44 PM, 8/22/2021] Lydia cUBe Drosphila: Looking forward to the same!
[1:46 PM, 8/22/2021] Lydia cUBe Drosphila: Okay
So why not send us your plan in detail.
Tell us, larvae from which culture bottle you are preferring…details of that culture bottle.
Send us your detailed plan for the olfactory assay and of the culture bottles that you’re going to use.
[1:49 PM, 8/22/2021] Lydia cUBe Drosphila: So what’s the story of the B20 bottle? From where did you transfer the flies into B20?
~20 days old flies are not that old, right @Aswathy Suresh CUBE?
Also, what’s the lifespan of a fruit fly? How long can a fruit fly live?
[1:52 PM, 8/22/2021] Lydia cUBe Drosphila: Nice!
Please send pictures of the 3 stages of fruit fly larvae, where we can clearly distinguish between the stages. It will be helpful for you as well as for others.
Also, what are the main features or characteristics that you look for in a larva to identify its stage?
Look for the tiny external head, three thoracic segments, eight abdominal segments, and a telson
extending beyond the anus. These segments are delineated by ventral rows of tiny hooks, denticle
belts, which prevent the larva from slipping backward as it moves forward with waves of extension
and contraction. You should also see a number of sensory bristles in the first instar larva distributed
over the cuticle.
[2:43 PM, 8/22/2021] Shraddha Sonavane CUBE: Sir, I only remembered about @Arunima’s work.she had observed Psyche butterflies larvae in her home lab during lockdown. But I don’t know exactly what was work done by Drishya.
[2:43 PM, 8/22/2021] Shraddha Sonavane CUBE: @KIRAN CUBE mam help in
1.set ups
2.In ecological survey of three different plants in Whole HBCSE campus.
3.during adult butterfly catching from HBCSE garden.

@KIRAN CUBE
@Yash Sheregare CUBE & @Shivam CUBE NEMATODE helped me by asking different questions in causeries, also in observations mainly during when butterflies was emerged from Pupae & in making poster for Science day presentation at TIFR, Mumbai on 29 Feb 2020.
Sun, Aug 29 at 11:00 GMT+05:30 Online Event Redirecting...

A gustatory receptor involved in host plant recognition for oviposition of a swallowtail butterfly
Authors- Katsuhisa Ozaki, Masasuke Ryuda, Ayumi Yamada, Ai Utoguchi, Hiroshi Ishimoto, Delphine Calas, Frédéric Marion-Poll, Teiichi Tanimura & Hiroshi Yoshikawa .
Published: 15 November 2011 https://doi.org/10.1038/ncomms1548
Female butterflies lay eggs on their host only when they detect specific chemicals through their foreleg chemosensilla while drumming on the leaf surface. Here we show that the butterfly, Papilio xuthus, uses a gustatory receptor specific for synephrine to select its host in oviposition behaviour.
@Shraddha Sonavane CUBE Please coordinate with @Arunima SN Nattika Cardamine to tell us more about Cardamine- Psyche butterfly interaction. @Shraddha Sonavane CUBE

ALERT:-
From last year’s data collected by Cubists, we are having a Hypothesis that Mango Flowering start from south Kerala and

Hence, collecting data of what mango trees does now onward is very useful to test the Hypothesis of Cubists.

All cubist are hereby requested to look around for MANGO FLOWERING in their locality and send pictures Of mango tree with NAME OF DATA COLLECTOR, NAME OF LOCALITY LAB, DATE, TIME, LONGITUDE AND LATITUDE, and MINIMUM AND MAXIMUM TEMPERATURE.

Crucial time for Mango Mapping!!
Looking forward for collaboration from all cubists across India!

Comparing with diagrams too there are two bulb like structures in my nematode instead of swollen pharynx please see the whole video

VIDEOS SHARES BY 7.37PM
16day old nematode culture shows good growth of nematodes potato is almost degraded .22/8/21Cubehomelab Kolkata BatulP
Nematodes in subculture B cultured with milk added on first day on raw potato and nematodes transferred from original raw potato slice22/8/21Cubehomelab Kolkata BatulP
Nematodes growing on raw potato slice subcultureC started on 16thAug21.Milk was added once again yesterday to help in growth of nematodes so the surface is looking white.Nematode can be seen on the marked area.22/8/21Cubehomelab Kolkata Batul P

[11:38 PM, 8/22/2021] Aswathy Suresh CUBE: Hypothesis is that lower latitude going upper latitude region there is kind of difference can able see in mango flowering. Based on last year observation came from Thiruvananthapuram - Kerala, 8.5241° N part mango started flowering on september last week onwards. Thrissur - Kerela 10.5276° N started mango flowering observed on October last weeks onwards. Going to upper part like Mumbai 19.0760° N - November/December , Kolkata 22.5726° N its taking December/January (If iam wrong please correct it). This is a kind of pattern is repeating or not in every year (Circannual Rhythm) this is we are addressing questions. We need to check this throughout collecting data from different parts of country.
[11:50 PM, 8/22/2021] Aswathy Suresh CUBE: Kanyakumari is 8.0883° N, 77.5385° E.
Trivandrum is 8.5241° N, 76.9366° E

Kanyakumari & Trivandrum are 8N away from equator (0N). But there is not much drastic change can’t able to see between these of them 8.0N - 8.5N.

Prediction : Kanakumari mango flowering expecting in first week of September onwards. In Trivandrum - mango flowering expecting in last week of September.
Nice picture We can able to see some Fruit flies present inside the Guava fruit.
Yes sir! We can search for wet places. Earthworms mostly found in nutrient black soil.
Awesome .

Why fruit flies are attracted towards cloth ? Because of smell of yeast ? What are they doing on clothes ?
[12:05 AM, 8/23/2021] Seethalakshmy CUBE: Yes sir! For culturing them cellulose as well as water is enough
[12:07 AM, 8/23/2021] Seethalakshmy CUBE: Sry sir! I didn’t get the question
[12:09 AM, 8/23/2021] Seethalakshmy CUBE: common name: crazy ant
scientific name: Paratrechina longicornis (Latreille) (Insecta: Hymenoptera: Formicidae)

https://entnemdept.ufl.edu/creatures/urban/ants/crazy_ant.htm
The crazy ant is so morphologically distinctive that it is one of the few Paratrechina that is not consistently misidentified in collections (Trager 1984). The crazy ant worker is relatively small (2.3-3 mm). The head, thorax, petiole, and gaster are dark brown to blackish (Creighton, 1950); the body often has faint bluish iridescence. The body has long, coarse, scattered, suberect to erect, grayish or whitish setae (hair-like projections).
And also their rapid movement as well as white bands
Yes we needs more improvement for identifying the genus and species level.
As well as we needs to study it’s morphology
[12:15 AM, 8/23/2021] Saida Sayyed: Breaking news
I lost all my Moina bottle culture

The tray in which I keep Moina fell over
[12:15 AM, 8/23/2021] Saida Sayyed: Currently, I have 2 tanks culture
30 Moina in each
Ok sir!
Immediative objective : To culture the Ants

Short term objective : To study it’s morphology.

Long term objective : How do ants communicate
Coordinating Growth and Review Maturation — Insights from Drosophila - PMC.
[12:35 AM, 8/23/2021] Aswathy Suresh CUBE: B20 flies I transferred from B19 Bottle - subculture of B line. Yes ma’am, those flies are not much old flies that’s also freshly got new flies from B19 bottle.
[12:36 AM, 8/23/2021] Aswathy Suresh CUBE: Life Span of Drosophila 70 Days - 90 Days.
[12:37 AM, 8/23/2021] Aswathy Suresh CUBE: A typical, healthy and well-maintained outbred Drosophila population will have a median lifespan of approximately 70 days and maximum of approximately 90 days at 25 °C. At a more detailed level of physiological decline, numerous markers of ageing-related loss of function can be observed.

https://www.sciencedirect.com/science/article/pii/S0925443917303319#:~:text=A%20typical%2C%20healthy%20and%20well,of%20function%20can%20be%20observed.
[7:46 AM, 8/23/2021] Lydia cUBe Drosphila: So by this, what do you think?
Can you please tell the transfer dates of B19, B20 and B21?
By this we can say how old the flies were in bottle B21.
[7:48 AM, 8/23/2021] Lydia cUBe Drosphila: What do you think of this claim? Do you think it’s right? How long does your flies live? Do your flies live for 70-90 days? And how does the lifespan of fruit fly depend on the temperature?
[7:49 AM, 8/23/2021] Lydia cUBe Drosphila: Ohh, how do you know that ants communicate?
Is there any particular behavior that we can notice and say that these ants are communicating?
[7:53 AM, 8/23/2021] Lydia cUBe Drosphila: Nice!
Please share the reference link to this picture.
Have you seen or identified these characteristics in the larvae in your culture bottles?
[7:59 AM, 8/23/2021] Lydia cUBe Drosphila: What do you think @Aswathy Suresh CUBE?
What could have happened?
It’s not flowering nor fruiting now!

I’ll try to get as many photos and samples to confirm that not flowering and fruiting is normal with mango trees in Jamshedpur in the month of August!

VIDEOS SHARED BY 10.30AM
*BREAKING NEWS Model organism C elegans can be cultured on raw potato slice with milk added to it on first day.Successfully maintained culture started on 29thJuly21,and subsequent subcultureB started on 6thAug,21 and subcultureC started on 16thAug,21 till date.video below shows nematodes growing in subcultureC today 28/8/21 Cubehomelab Kolkata Batul P
[11:04 AM, 8/23/2021] Aswathy Suresh CUBE: I didn’t felt that fruit flies live for 70-90 days. Because I never seen it in my culture. Also my culture is mixed with young and old flies. And every subcultures bottles after 15 days later can able see ~30 dead flies. This means flies have only ~30days life span, I suspect.
[11:05 AM, 8/23/2021] Aswathy Suresh CUBE: B19 - Transfer date 02.07.2021

B20 - Transfer date 19.07.2021

B21 - Transfer date 12.08.2021
[11:08 AM, 8/23/2021] Aswathy Suresh CUBE: I think the smell of fermented yeast, attracted these flies towards clothes.
[11:49 AM, 8/23/2021] Abhijeet Singh: Is there a story behind expecting approx, 50 eggs and larve and pupae and ~40 flies on the 9th day?
What was your idea behind your expectations!?
@Aswathy Suresh CUBE
[11:53 AM, 8/23/2021] Abhijeet Singh: Yes we can!!
With the help of blackpaper as background it’s clearly visible!!
I’ve posted the photos too!
Please follow the thread to see the photos.
If you don’t find it I’ll send it again!!
[12:05 PM, 8/23/2021] Abhijeet Singh: Cellulose is mainly used to produce paperboard and paper. Smaller quantities are converted into a wide variety of derivative products such as cellophane and rayon.

Cellulose for industrial use is mainly obtained from wood pulp and cotton.

Some animals, particularly ruminants and termites, can digest cellulose with the help of symbiotic micro-organisms that live in their guts, such as Trichonympha. In human nutrition, cellulose is a non-digestible constituent of insoluble dietary fiber, acting as a hydrophilic bulking agent for feces and potentially aiding in defecation.
Reference:-

Cellulose

VIDEOS SHARED BY YASH 12.48PM
Today’s Headline - 16th Day update of SLPbA2 sub culture shows 12 fruit flies, few larvae, pupae and plenty of dead fruit flies!
Media in SlPbA2 is liquidy as compared to SLPbB2 and C2!

Media date - 06/08/2021
Transfer date - 7/08/2021
No of flies transferred on 7/08/2021 - 35

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality

23/08/2021
No of flies -12
No of larvae - 12- 3mm larvae, 5 - 2mm larvae
No of pupae - 20
No of dead flies - 30 @Aswathy Suresh CUBE @Arunan HBSCE sir @Lydia cUBe Drosphila @Isha Pawle and others…
Today’s Headline - 16th Day update of SLPbB2 sub culture shows 40 fruit flies and plenty of larvae and pupae.

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality

Media date - 06/08/2021
Transfer date - 7/08/2021
No of flies transferred - 30

16/08/2021
No of flies - 40
No of larvae - 10 - 3mm larvae, 5- 2mm larvae, 10 - 1mm larvae
No of pupae - 40 unhatched pupae
No of dead flies - 20
Today’s Headline - 16th Day update of SLPbC2 sub culture shows 50+ fruit flies and plenty of larvae of all stages and pupae.

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality

Media date - 06/08/2021
Transfer date - 7/08/2021
No of flies transferred - 30

23/08/2021
No of flies - 50+
No of larvae - 10 - 3mm larvae, 15- 2mm larvae, 10 - 1mm larvae
No of pupae - 10 unhatched pupae
No of dead flies - 10-15
Wow! @Aswathy Suresh CUBE Congratulations!
Did we just save the A line culture by transferring the gravid fruit fly to new media bottle!?
@Lydia cUBe Drosphila @KIRAN CUBE
We remember there were no larvae in A21 culture 10 days back and now there are plenty of eggs and larvae in new A22 sub culture!
PubMed Central Image Viewer. WoW! This nematode cultured by @Batul Pipewala Kolkata maam looks like C elegans according to the reference above! @Anshu Kadam (Nematodes) @Shivam CUBE NEMATODE what do you think?
Mango tree not started flowering!

Flowering not started
No sprouting
No Fruiting Yet.

Date : 23.08.2021,
Time : 12:17 PM

Latitude: 10° N Longitude: 75° E

Temperature :
Maximum : 32°C
Minimum: 28°C

Location: Ponnani, Malappuram, Kerela
CUBE Homelab Ponnani.
Data Collector: Amal kallingal
Sir I’m expecting flowering to start by September.
I’ll keep getting updates and I’ll add the observation in the given link!!
This is my expecting from one gravid fly that transferred from A21 to A22 after 9th day.

One gravid fly lay 50 eggs per day (Referance says) . In this calculation we will get 450 eggs on 9th day. Also get ~50-60 progeny’s get in 9th days.

But usual observation eggs are present inside cultures ~20-40 is maximum can able see but it fertilized or have not no idea because Larvae present in culture very less.[3:07 PM, 8/23/2021] Aswathy Suresh CUBE: Yes @Yash Sheregare CUBE, Eggs Can able to see inside the culture ~20-40, But larvae are very less present culture. Only 3 larvae I can able to see inside culture.
[3:07 PM, 8/23/2021] Aswathy Suresh CUBE: Expecting some more larvae will come out from these eggs.
How many days old culture bottle is this @Yash Sheregare CUBE?

When do you expecting pupae from this bottle? @Man Masih Beck RANCHI CUBE

[3:27 PM, 8/23/2021] DP HBCSE:
[3:28 PM, 8/23/2021] DP HBCSE: Some have already started! Is this to the South of your place @Abhijeet Singh​?
Expecting pupae on 26th Aug(7th day of transfer)
butterfly (psyche )can connect with Cardamine
Great capture @Batul Pipewala Kolkata ma’am

But I still feel that the part which you are claiming to be 1st bulb, doesn’t look like a bulb to me.

@Yash Sheregare CUBE @Arunan HBSCE @KIRAN CUBE and others
If you see the whole video rather than still photo you may be able to see a bulb rather than swollen pharynx
Pruitfly on Apple piece in wine glass at CUBE HomeLab Bhulabhai Desai Road, Mumbai 23/8/08/2021 Photo: mcarunan

VIDEO SHARED BY SEETHALAKSHMI AT 6.51AM

Phone microscopy to see nematodes on a glass slide using flash below
Nematodes moving on potato surface caught by mobile camera and a magnifying glass

VIDEOS SHARED BY BATUL AT 9.11PM
Picking up a nematode to transfer to another potato slice

The Natural Biotic Environment of Caenorhabditis elegans

[KiranKalakotiR (Kiran Kalakoti)]

23rd August 2021, 9.30pm to 24th August 2021, 9.30pm
Mango tree not started flowering!

Flowering not started
No sprouting
No Fruiting Yet.

Date : 23.08.2021,
Time : 6:35 PM

Latitude: 19.0237° N, 73.0404° E

Temperature :
Maximum : 25°C
Minimum: 31°C

Location: CBD Belapur,(Mango Garden) Navi Mumbai, Maharashtra
CUBE Homelab CBD Belapur
Data Collector: Swayam Gaikwad
Mango tree 2 not started flowering!

Flowering not started
No sprouting
No Fruiting Yet.

Date : 23.08.2021,
Time : 6:35 PM

Latitude: 19.0237° N, 73.0404° E

Temperature :
Maximum : 25°C
Minimum: 31°C

Location: CBD Belapur(Mango garden) Navi Mumbai, Maharashtra
CUBE Homelab CBD Belapur
Data Collector: Swayam Gaikwad

VIDEO SHARED BY DRISHTANT AT 12.20AM
Fungus in its own niche captured via mobile microscopy
Growing on an empty stalk of a custard apple.
Are these conidia? What do we call the white filament-like structures?

Taken via a convex lens obtained from laser light.
No digital zooming was done.

Magnification: Unknown
Place: Nerul, Navi Mumbai
Date: 23rd August 2021
Video: Drishtant MK
White stalk is Sporangium and tip bulbous round structure is Sporangiophore inside of which is spores r present
Great
What do you mean by non - moving larvae like structure? Is it pupae?

Cylindrocladium leaf and fruit spot of custard apple in Queensland -...

This is beautiful. It is tough to imagine that ugly looking fungus can appear so good when magnified. What are the typical features by which we distinguish fungus?

Mushrooms also have a similar structure. Do their heads also have spores? Or am I completely off?
[8:59 AM, 8/24/2021] Arunan HBSCE: Can we get a longer shot where this set up is placed? @Batul Pipewala Kolkata
[9:07 AM, 8/24/2021] Arunan HBSCE: " This nematode is particularly abundant in microbe-rich environments, especially rotting plant matter such as decomposing fruits and stems. In this environment, it is part of a complex interaction network, which is particularly shaped by a species-rich microbial community. These microbes can be food, part of a beneficial gut microbiome, parasites and pathogens, and possibly competitors. C. elegans is additionally confronted with predators; it interacts with vector organisms that facilitate dispersal to new habitats, and also with competitors for similar food environments"
" Full appreciation of this nematode’s biology warrants further exploration of its natural environment"
A great introduction @Batul Pipewala Kolkata to look for C elegans in Indian/ Srilankan soil @Batul Pipewala Kolkata @Anshu Kadam (Nematodes) @Ishita Assam
[9:11 AM, 8/24/2021] Arunan HBSCE: Please give reference link too @Ankit Yadav Xms Work Shop Cube Patkar College
Please give a description @Dr. G C Baskey Cube Ranchi
Please send the photos, Videos and data for the CHAI and WHY?
Fruitfly:- @Aswathy Suresh CUBE @Isha Pawle @Yash Sheregare CUBE @Ishita Assam
Cardamine:- @Arunima SN Nattika Cardamine @Shraddha Sonavane CUBE @Rechel Turkey Cube Ranchi Moina @Nazish Cube Drosophila @Lakshmy PJ CUBE
Nematodes:- @Arunima SN Nattika Cardamine @Anshu Kadam (Nematodes) @Ishita Assam
Phyllanthus:- @Man Masih Beck RANCHI CUBE @Lakshmy PJ CUBE @Shraddha Sonavane CUBE @p.chitralekha
Yeast:- @Seethalakshmy CUBE @Aditiya Joshi CUBE
[9:22 AM, 8/24/2021] Arunan HBSCE: Why on 7th day?@Man Masih Beck RANCHI CUBE What’s the reasoning? @@Man Masih Beck RANCHI CUBE @Aswathy Suresh CUBE
[9:24 AM, 8/24/2021] Arunan HBSCE: Please make your argument more clearly using some reference too @Shivam CUBE NEMATODE
This looks fine! @Lakshmy PJ CUBE
[9:27 AM, 8/24/2021] Arunan HBSCE: Time and date please! @Lakshmy PJ CUBE
[9:29 AM, 8/24/2021] Arunan HBSCE: Good to see you back @Nazish Cube Drosophila

Colonies on boiled potato slices

Few colonies on boiled potato slices

Boiled potato slices in a plastic box

Dipping cotton buds for spreading fermented coconut water on boiled potato slices. Aditya Joshi, Kalyan west

Bottles kept for fermenting coconut water. Aditya Joshi. Kala Talav, Kalyan west.

Phyllanthus growing in pots n plastic box. Aditya Joshi. Kala Talav, Kalyan west.

These are few photos of of toddy experiment setup n colonies and PhyllanthusAfter how many days? Please post a summary starting with Objectives @Aditiya Joshi CUBE and interpretation.
Objectives and some essential summary including further plan of work, please @Aditiya Joshi CUBE
Objective : To study the effect of Continous light on sleep -wake cycle of Phyllanthus

Plants kept in grill,store room and under the table
Can we please get previous national meet videos

Fruit Flies are present on Tomato kept fruit basket observed in kitchen.

Picture Credit : Shaharubanu CUBE SN Nattika.
Date : 24.08.2021, Time : 11:46am

Fruit flies observed in kitchen garbage bin lid area.

Picture Credit : Shaharubanu CUBE SN Nattika
Date : 24.08.2021, Time : 11:51am

Fruit Fly - On Pineapple Slices

Picture Credit : Shaharubanu CUBE SN Nattika
Date : 24.08.2021, Time : 11:57am

Close Up Picture Of Fruit Fly.
Picture Taken : Shaharubanu CUBE SN Nattika.
Date : 24.08.2021, Time : 11:46am

While walking through my local park I saw a slug (although it was alone) on the pavement. Does this mean a future revival can take place for the Cube Ghatkopar Snail homelab as snails and slugs are similar to one another and belong to the same class gastropoda​:flushed:

VIDEO SHARED BY RECHEL AT 12.05PM
The floral dip method by Ranchi cubists @⁨Manisha Di Botany⁩ @⁨Dr Shalini Lal⁩ before lockdown. This was also presented in previous CUBE national meet.
Slides used to make the video for cardamine floral dip method
Uploading: DR2.jpeg…

Width of leaves of different species of Phyllanthus plants. location-Panvel.Data collected by-Shraddha

Cardamine plants from home lab, Panvel. 3 simple leaves & 1st compound leaf with partially developed & differentiated leaflet ,2nd compound leaf with completely differentiated leaflet
Cardamine plants grown without soil (on moss ) showing Cardamine & moss interactions. Location- Panvel data collected by-Shraddha

Phyllanthus urinaria plants kept in continuous light experiment.

Phyllanthus plants kept in continuous dark experiment

Phyllanthus urinaria characteristics features.

Please send photos, video, presentation of previous national meet in the group as soon as possible!
@Nazish Cube Drosophila @Arunima SN Nattika Cardamine @Yash Sheregare CUBE @Drishtant Cube Moina @Anshu Kadam (Nematodes) and others!!

How to compare & identify Phyllanthus plants from other leguminous plantsSeeds of Phyllanthus urinaria plants

Home lab set up for Continuous light and dark experiment in Phyllanthus urinaria plants.

A picture from earlier video of National Meet 2020. The 6 Nobel Prize Winners and the 6 Time Noble Prize winning Model system - Fruit fly!

Phyllanthus urinaria plants kept in normal sunlight as control for continuous light and dark experiment

How to compare & identify Phyllanthus plants from other leguminous plants
Phyllanthus urinaria.
Data collected by: Theertha
Date:24/8/2021
Place: Cherukkad, Kozhikode.
CUBE SN Nattika
Phyllanthus amarus.Data collected by: Theertha.
Place: Cherukkad, Kozhikode
Date:24/08/2021
CUBE SN Nattika
Phyllanthus debilis.Data collected by: Theertha.
Place: Cherukkad, Kozhikode
Date:24/08/2021
CUBE SN Nattika

Fruit fly culture bottles with TRSV medium. Date: 01/09/2020. Location: Thanniam , Thrissur, Kerala. Sidhy.P.P , CUBE S N College, Nattika.

Cardamine plant .close relative of arabidopsis thaliana.

Cardamine plant development of floral buds to seeds

Preparation of TRSV medium for fruit fly culture. CUBE Home lab Thanniam, Thrissur , Kerala. By Sidhy.P.P , CUBE S N College, Nattika.Chai and why? Nematodes – Google Drive

Nematodes @Chai and why@@Saida Sayyed @Abhijeet Singh
Preparation of 150 ml of TRS-V Medium

Ingredients
Water​:cup_with_straw:( 150 ml)
Egg Size Tomato (~50 gm)
2 table spoon Rava​:rice:(20 gm)
1table spoon Sugar (15 gm)
Vinegar (15ml)

Protocol
1.Taken a pan. Turn on the flame.
2. Add ~150ml water ,2 table spoon rava ,1 table spoon sugar.
3. Then add 1smashed tomato.
4.Mix continuously for 10 minutes at low flame .
5. Turn off the flame and leave it for cooling for 5mins.
6.Then add ~15ml Vinegar in medium and mix it well.
7. Pour in to clean and dry bottle and add cotton plug to it.
8. Leave it for solidification for 24hrs
9. After 24 hrs transfer flies to the media bottle.

Video Credit- Isha Pawle
While uploading the Google drive link in the group Please make sure that Google drive folder includes all the photos videos and all the stuff of the particular model organization of all the collaborators working on in cube on that!

the excess to the Google drive link should be to everyone
Mobile Microscopy​:microscope:

To observe the fly using any microscope we first need to immobilise the flies.
Transfer some flies from a single line culture into a empty bottle.
Keep this bottle into refrigerator.
After 1 hours Remove the bottle and we can see flies getting immobilise.

Requirements For Mobile Microscopy
1- Torch🔦
2- Mobile camera📱
3- White Sheet Papar📄
4- Glass Sheet or a Transparent sheet Paper
5- Needle
6- Brush
And 7- Immobilised Flies

Setup for Mobile Microscopy
Starting from the bottom first place a TORCH
Above the torch place A WHITE SHEET PAPER
Then above it place a GLASS SHEET.
At last place our object i.e. the FLY
Take a MOBILE CAMERA and adjust the focus accordingly. We can zoom in or zoom out to get a clear picture of the fly.

Video credit - Isha Pawle

Seeds harvested from cardamine plant

Phyllanthus model system.
Identification
Plant : Phyllanthus tenellus.
Long stalked phyllanthus.
Erect annual herb.
Leaves:
Alternate,leaf hair absent,oblong in shape(subacute -leaf tip,acute to rounded - leaf base)
Fruit :
Capsule:Smooth.
On short stalked .
Flowers with 1.5-5 mm long stalks.
5 tepal.Stem-irregular round

Google Sites: Sign-in

Date:24/08/2021.
Place: Cherukkad,Kozhikode.
Data collected by: Theertha.
CUBE SN Nattika.
https://drive.google.comhttps://drive.google.com/file/d/14isoHZ6A0ZHiznkyaUh0-kqJaXhtxxGw/view?usp=drivesdk
Title: "Isolation of soil nematodes from soil sample on a raw potato surface in Home labs”

https://drive.google.com/file/d/1kCklrqGWlJ7ji9ont0iDgOZA6-5ozFGG/view?usp=drivesdk[6:53 PM, 8/24/2021] Sunita Chahar CUBE: Phyllanthus niruri: Benefits, uses, and risks
[6:57 PM, 8/24/2021] Sunita Chahar CUBE: The Genus Phyllanthus: An Ethnopharmacological, Phytochemical, and Pharmacological Review
[6:59 PM, 8/24/2021] Sunita Chahar CUBE: https://www.cabi.org/isc/datasheet/46049
[7:01 PM, 8/24/2021] Sunita Chahar CUBE: http://www.bioinformaticssikkim.gov.in/medicinal%20Plants/131.html

rapping Fruitflies for Studying the Activity Pattern of Fruitflies in July 2018 - Near the biogas plant kachada dubba n can with fermented rice water near by to attract fruitfly.

Photo: Sulekha Sudhakaran, CUBE Locality Lab, Paruthiara, a CUBE Node of the CUBE Hub, Vennicode ( lThiruvananthapuram Dist.).

CUBE Ranchi Re-Mans’ Home Lab
24-08-2021
Fruitfly Update

Breaking News
2 pupae observed on the 6th day of transfer

Immediate Objective: to start a single line fruitfly culture(RNSL-B) at ReMan’s Lab in TRSV Media

Short Term Objective: -To identify the trapped fruitflies
-To get native Drosophila melanogaster species

Media preparation- 18-08-2021
Transferred date- 19-08-2021

OBSERVATION
on the 6th day of transfer
•the transferred fly
•~16 larvae
•~2 pupae
•~1 fly

Expectation
-Earlier Expectation: New flies will emerge on 27th Aug, 2021(pupae developing on 26th Aug, 8th day of transfer)
-New Expectation: New flies will emerge on 25th Aug, 2021(pupae developing on 6th day of transfer) ​@Aswathy Suresh CUBE @Arunima SN Nattika Cardamine @Drishtant Cube Moina

[KiranKalakotiR (Kiran Kalakoti)]

24th August 2021, 9.30pm to 25th August 2021, 9.30pm

VIDEO SHARED BY SIDHY AT 11.35PM
SLTT-H
Single line culture of fruit flies.
Started on 16/08/2021, 11:40 AM
TRSV medium - 15/08/2021, 11:00 PM

Expectation:
Pupae on fourth or fifth day 20 , 21/08/2021
New flies will emerge on 8 th day ( 24/08/2021)

Observation on 24/08/2021
~ 30 larvae
~ 21 pupae
~12 flies

Location : Thanniam, Thrissur, Kerala
Sidhy.P.P, CUBE S N College , Nattika.
SLTT-I
Single line culture of fruit flies.
Started on 16/08/2021, 11:40 AM
TRSV medium - 15/08/2021, 11:00 PM

Expectation:
Pupae on fourth or fifth day 20 , 21/08/2021
New flies will emerge on 8 th day(24/08/2021)

Observations : Pupae on 6 th day.
24/08/2021- ~ 40 larvae ,10 pupae ,No new flies emerged.

Location : Thanniam, Thrissur, Kerala
Sidhy.P.P, CUBE S N College , Nattika.
SLTT-J
Less number of larvae and pupae on 8 th day

Single line culture of fruit flies.
Started on 16/08/2021, 11:40 AM
TRSV medium - 15/08/2021, 11:00 PM

Expectation:
Pupae on fourth or fifth day 20 , 21/08/2021
New flies will emerge on 8 th day(24/08/2021)

Observations : Pupae on 6 th day.
24/08/2021- ~ 10 larvae , 2 pupae ,No new flies emerged.

Location : Thanniam, Thrissur, Kerala
Sidhy.P.P, CUBE S N College , Nattika.
In this video i can see your voter mark
Is it still there?

By looking at the Pupae what do you predict?
When are you hoping to see flies coming out of pupae? @Sidhy Kerala Drosophila
Can you post some close up photos of pupae?
[8:55 AM, 8/25/2021] Arunan HBSCE: Why not celebrate by giving a BREAKING NEWS STORY with HEADLINES? @Sidhy Kerala Drosophila
[9:03 AM, 8/25/2021] Arunan HBSCE: Wonderful observation! A fine detective work like that of Sherlock Holmes! @Seethalakshmy CUBE @Sidhy Kerala Drosophila
Almost 120 day’s the Voter’s mark remains in this Nail! Wow! @Sidhy Kerala Drosophila @Seethalakshmy CUBE

By looking the pupae , the expectation was that the flies will come out of the pupae today 25/08/2021. In SLTT- I and SLTT - J , one fly came out of the pupae today morning. These pupae were formed on 22/08/2021. There had 5 pupae formed on same day. From 2 of them flies came out. Hope that the remaining 3 flies also will come today.
From the pupae (in the photo) the flies will come out soon.
What would you write as a BREAKING NEWS HEADLINE? @Sidhy Kerala Drosophila

BREAKING NEWS 118day old nail mark !! Date of mark 29/4/21 date of pic 25/8/21 Gender F, Age53, No of days 118days, Growth of nail 12mm.Cube Homelab Kolkata BatulP
Not much difference in nail growth over 120days between 53yr old female and @Sidhy Kerala Drosophila female age 21
Pic of 120day old nail mark shows growth of 13mm@@Sidhy Kerala Drosophila
New generation on 9 th day in SLTT-I and SLTT- J. One day later than the expectation.

The expectation was pupae on 5 th day and flies on 8 th day. What happened is , pupae formed on 6 th day and flies emerged on 9 th day. The period from pupae to fly is same as the expectation. Flies emerged on third day after the formation of pupae.

SLTT- H , which started along with I and J , got new generation on 8 th day
New Generation of Fruitflies from Single Motherfly? @Sidhy Kerala Drosophila
Before the Pandemic Fly culture 20 Feb 2020 CUBE HBCSE Photo : CUBE Archives mcarunan

Phyllanthus closing leaves at 6:02 pm From CUBE Archives 2019 mcarunan Please see the next pic.Yes Sir. SLTT-H , SLTT-I and SLTT-J are newly started single line cultures of fruit flies. Transferred one gravid fly to each bottle on 16/08/2021.
Phyllanthus leaves open at 9:02 am From CUBE Archives 2019 mcarunan Please see the previous pic.

Whiteboard screenshots of Sleep wake cycle of fruit fly and opening and closing of Phyllanthus plant.
On Wikipedia page, they mentioned that Psyche butterflies larvae feeds on Capparis but Cardamine plants is not mentioned there. This is screenshot of Wikipedia page.
Invitation to join today’s CUBE chatShaala,
Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in all CUBE what’s app groups and on metastudio after the session

Here’s the direct link of the CUBE webinar: https://webinar.hbcse.tifr.res.in/b/kir-hye-cn4

Note: Today we are delaying the CUBE chatShaala for an hour

Timing:6.30-9.30pm
Looking forward to your participation
Thanks.
Attention!
For the Chai and Why? we need photos of homelab setup with the back of your home environment. As depicted in the sample picture that i have attached! The photos should reciprocate the idea that yes Homelab are actually at home and nothing sophisticated is required for science
Kind Request to all the CUBists to send the photos as soon as possible because we have to put that photos in the presentation for ‘Chai and Why?’ for the mock presentation that is tomorrow.

Thank you!

[KiranKalakotiR (Kiran Kalakoti)]

25th August 2021, 9.30pm to 26th August 2021, 9.30pm
Using potato, damp soil, damp tissue paper for observing nematodes.
[11:50 PM, 8/25/2021] Lydia cUBe Drosphila: That’s true
You have a mixed culture of young and old flies
Do you think you can plan something to find out how long fruit flies can live/their lifespan? What say?
Do you think knowing the lifespan of fruit flies is important?
[11:54 PM, 8/25/2021] Lydia cUBe Drosphila: Okay!
B19 indicates that this is the 19th culture bottle of single line B…right?
So you had B1, B2 and so on and now till B21…right? B1 started with 1 gravid fly…
And then their progenies are being transferred into the further culture bottles…right?
Just correct if I’m wrong anywhere
What happened to the initial culture bottles of single line B? Do you have any or do you only have the cultures B19, B20 and B21?
[11:55 PM, 8/25/2021] Lydia cUBe Drosphila: One more piece of information is required.
Can you please tell us the number of flies transferred to culture bottles B19, B20 and B21
[11:56 PM, 8/25/2021] Lydia cUBe Drosphila: What particularly in the smell of fermented yeast, attracted the fruit flies?
[11:58 PM, 8/25/2021] Lydia cUBe Drosphila: Nice!
Understood!
What is your objective with these mosquitoes?
(I’m sorry if I missed out on this too in the previous messages…not able to keep a track of all the messages these days)
Interesting!
A twist in the conversation! Which microorganisms live in the guts of ruminants? Maybe an example can help!
Also, can these microorganisms be used as cellulose digesters for some bio-based aspects or experiments ?
What do you think? Even I don’t know about this…I was just wondering about this while reading your message

Do we consume any food that has cellulose in it?

High levels of cellulose are found in root and leafy vegetables, legumes, and some fruits such as pears and apples
https://www.nap.edu/read/1222/chapter/13#293

Green beans were highest in cellulose and hemicellulose

Dietary fiber constituents of selected fruits and vegetables - PubMed

We do consume some of these foods often. What do you think? How do we digest or consume these foods even though we don’t have the ability to do so? Won’t this be a problem ?
What do you think could be a reason for the death of fruit flies @Yash Sheregare CUBE? Even @Aswathy Suresh CUBE is facing the same issue.
[12:19 AM, 8/26/2021] Lydia cUBe Drosphila: These are working well @Yash Sheregare CUBE
SLPbB2 and SLPbC2 cultures
Then why is SLPbA2 having an issue?
[12:23 AM, 8/26/2021] Lydia cUBe Drosphila: This is good news! @Aswathy Suresh CUBE
Hoping to see and get more updates on this new A22 culture bottle
[12:25 AM, 8/26/2021] Lydia cUBe Drosphila: I think cardamine is a host plant for one butterfly species, right @Shraddha Sonavane CUBE?
That way we can connect I guess.
[12:27 AM, 8/26/2021] Lydia cUBe Drosphila: Yes
Life cycle of different model organisms can be connected…this comes under the curriculum topic, developmental biology
Yes @Lydia cUBe Drosphila, We can connect through butterfly Cardamine interactions.
Because Cardamine is host plants of Psyche butterflies.

VIDEO BY SEETHALAKSHMI AT 12.59PM
Update on ANT Model system

Immediative objective : To culture the Ants

Short term objective : To study it’s morphology.

Long term objective : How do ants communicate
Location : Pazhuvil
Date:25/8/21.
Time:9:51 pm
Data collector : Seethalakshmi
Have already sent earlier attached in drive
Some are given by me. Of phyllanthus and toddy. If needed, I will send more

Frugal HomeLab at South Mumbai, 26 August 2021
Nematode subcultureC started on 16thAug,21, 11days later 26/8/21Cubehomelab Kolkata BatulP
[9:43 AM, 8/26/2021] Arunan HBSCE: How do we Design an experiment to find the Lifespan of Fruitflies? @Aswathy Suresh CUBE @Lydia cUBe Drosphila @Ishita Assam @Raj Yashwantrao Patil CUBE @Yash Sheregare CUBE to design and start to show this today for Chai& Why Mock session.
[9:44 AM, 8/26/2021] Arunan HBSCE: What do we mean by fermented yeast? @Aswathy Suresh CUBE
Wow! Flowering of Mango trees observed at 10.5°N at Thrissur, Kerala. @Aswathy Suresh CUBE What percentage of trees in this locality are flowering? @Aswathy Suresh CUBE
What is your further plan? @Yash Sheregare CUBE Please post a few good photos to show your HomeLab set up with Location and Date @Yash Sheregare CUBE
Ants release pheromones through which they communicate to fellow ants. That’s why ants move in a line…after one another.

During our childhood, we
used to disturb their sequence by moving fingers arround them. Ants used to disperse around and then again form a line.
Later on found out that we were dispersing the pheromones…disturbing their moving pattern…

It’s a very interesting phenomenon…
[9:56 AM, 8/26/2021] Arunan HBSCE: How about keeping a slide like this to show the extent of collaboration and a glimpse of concept map that all cubists work together, from across the country, to all at the end of Chai& Why? session, today?
[9:56 AM, 8/26/2021] Arunan HBSCE: @Saida Sayyed @KIRAN CUBE
[9:59 AM, 8/26/2021] Arunan HBSCE: Good, you transferred one fly to A22 bottle. Please tell us exactly what happened each day. @Aswathy Suresh CUBE
Excellent! @Man Masih Beck RANCHI CUBE Can you take videos of your activity like how you observe and Record the Data in your CUBE HomeLab from today as a video to be screened for Chai& We hy? session? @Man Masih Beck RANCHI CUBE Let’s start.[10:05 AM, 8/26/2021] Arunan HBSCE: Three larvae of SLBK A are great! Hope, you are following it up daily.

Please give update and post long-shot photos of your Lab Set up for Chai& Why? @Aswathy Suresh CUBE
[10:08 AM, 8/26/2021] Arunan HBSCE: Please take this observation asa movie how do you do at HomeLab. Let’s use it for Chai& Why to show how your lab world on a daily basis @Man Masih Beck RANCHI CUBE @Saida Sayyed
Excellent! @Aswathy Suresh CUBE What percentage of Mango trees in this locality is sprouting fresh leaves?
What percentage is flowering? Wonderful! @Aswathy Suresh CUBE
What percentage out of total trees are in this category? @Aswathy Suresh CUBE What is your Sample Size?
Beautiful! @Shraddha Sonavane CUBE
Can we develop this as a HomeLab experiment to show how exactly you
assemble all items to do this study for Chai& Why session? A movie will do. @Shraddha Sonavane CUBE Please collaborate @Aditiya Joshi CUBE @Lakshmy PJ CUBE @Shrudhiga
Surely more than 6persons eon Nobel Prize working on Fruitflies!@Yash Sheregare CUBE please clarify.
Long-shot photos to show the HomeLab set upar NewPanvel, please @Shraddha Sonavane CUBE
Please take a video on how do you do cold treatment to Cardamine seeds, showing your refrigerator, HomeLab set up for germination of seeds. @Arunima SN Nattika Cardamine Let’s use that for today’s hai &Why? mock session. @Arunima SN Nattika Cardamine
Please give a long-shot to show your Cardamine HomeLab set upay Kozhikode. @Arunima SN Nattika Cardamine
Date and also a long shotto show the HomeLab at your place @Sidhy Kerala Drosophila
Beautiful. Reference scale, Date,HomeLab location. Technique of Photography @Arunima SN Nattika Cardamine
Excellent! @Isha Pawle Can we have a on the spot movie of this transfer to show that we exploit the phenomena of Negative Geotaxis? @Ishita Assam
Dates and a few Long shots too, please @Arunima SN Nattika Cardamine
Longer shots with dates please! Wonderful😇 @Arunima SN Nattika Cardamine
We need to design a plan in order to find the life span of fruit flies - culture bottles ma’am.Here’s the short video of my fruit fly home lab.
From Worli, Mumbai.[10:42 AM, 8/26/2021] Aswathy Suresh CUBE: Definitely it’s important. Because Current situation facing major issue is flies dying in culture without any reason.we need to find out the answer. Before that we have to know how many days flies will alive (Life span)
[10:57 AM, 8/26/2021] Aswathy Suresh CUBE: Yes, B Line started on 26th May 2020 during the pandemic started time. started B line culture with 1 mother fly, trapped using tomato bait. Culture named SLBK B. Starting from there after several subculture passed B1,B2,B3,B4…B19, B20, B21 (subcultures) . Every months transferring all progeny’s from old media bottle to new media bottle in order to maintain that line culture. Last made subculture is SLBK B21 , Currently I have SLBK B18, B19, B20, B21 subculture bottles. But only B21 bottles have Larvae, Pupae, Adult Flies. Old bottles were taken for making new subcultures…cleaned and reused for making further subcultures (Because of unavailability of bottles).
[11:02 AM, 8/26/2021] Aswathy Suresh CUBE: I don’t have B subculture bottle which I started on 26/05/2020.That bottle were remained after 1 and half month. After that I taken that bottle and cleaned reused for making further subcultures.

Currently I have only B21 (Date of bottle : 12.08.2021) which have flies,larvae, pupae.
[11:03 AM, 8/26/2021] Aswathy Suresh CUBE: B Line
Great! @Isha Pawle We can make it shorter by editing some early part and show the long shot of the HomeLab setting. @Isha Pawle
Is this fine @Arunan HBSCE sir?
Total 5 mango tree surveyed. one of the mango tree started to flowering, another one started to sprouting. Rest of them (3 mango tree) no flowering, no sprouting, no fruiting.

26 August 2021. Fruit fly culture bottles in Home lab Thanniam,Thrissur, Kerala.
8 July 2020. Fruit fly Home lab Thanniam, Thrissur, Kerala. Sidhy.P.P , CUBE S N College, Nattika.
[1:15 PM, 8/26/2021] Arunan HBSCE: Looks fine. Last bit could be edited a few seconds more, I guess. @Isha Pawle
[1:16 PM, 8/26/2021] Arunan HBSCE: So what is the percentage calculation? @Aswathy Suresh CUBE
Can we get another still Long shot to see where in the house it is being maintained? @Sidhy Kerala Drosophila

Hello I am Binita. In June 2017 two months after returning back from HBCSE, I started first Kitchen lab. Undergraduate research is not yet popular in Jharkhand, so except @Dr. G C Baskey Cube Ranchi and @Shalini CUBE Rachi people were skeptical about undergraduate research. I wanted to check the hypothesis so I convinced my parents and family members. A space was needed for other people to discuss and perform science, we did several experiments in this space. 10 months after the lab started we got a space in college. If you ever thing that you are not able to do science because of some problem, then I am sure the problem in your head. Also I strongly believe that places and surrounding should not decide how fit we are for doing what we want to do. I got a chance to work in CCMB for master’s thesis in Dr. Rakesh Mishra’s lab. Currently I am taking a reading break, applying for PhD and running a VIRTUAL KITCHEN LAB. The idea is to gather people to share their journey in science. I hope you all will enjoy watching these videos. https://www.youtube.com/channel/UCXwh1LampcQbILWhqNmrynw There are some amazing videos that are coming up soon.
https://www.youtube.com/channel/UCXwh1LampcQbILWhqNmrynw
Home Lab - Bottukadavu Thrissur Kerala. Maintaining model organisms Fruit Flies, Moina.
[2:40 PM, 8/26/2021] Arunan HBSCE: Date please and repost with essential dryails. @⁨Aswath@Aswathy Suresh CUBE

[2:41 PM, 8/26/2021] Arunan HBSCE: details*
Date of photo : 26.08.2021
Time : 2:05pm
Photo taken : Aswathy Suresh CUBE SN Nattika.
Location : Bottukadavu - Thrissur Kerala.
Total surveyed Trees = 5
Flowering Mango trees = 1
Sprouting Mango trees = 1
Fruiting Mango trees = 0

% Flowering mango trees = 20%
% Sprouting mango trees = 20%
% No Flowering, Sprouting, Fruiting mango trees = 60%

Data Collector : Aswathy Suresh CUBE SN College Nattika.
Location : Bottukadavu - Thrissur Kerala.

A slice of the 1st day of CUBE National Meet 19, 20, 21March 2021 5-30pm to 9-30 pm https://webinar.hbcse.tifr.res.in/b/kir-vgc-6jf

https://www.youtube.com/metastudioCHAI AND WHY? SESSION
We are happy to announce that we have got the chance to be the part of Chai and Why?session organised by TIFR to share and narrate some of CUBE inventions that we came up collaboratively during the Pandemic .

Chai & Why? -TIFR’s unique outreach initiative since 2009 has been the “Chai and Why?” series, a forum to enable informal discussions of interesting scientific issues outside a traditional academic setting in a Science Café format.

We invite everyone to be the part of this session. The session would be conducted on zoom and would also be live telecast on YouTube

EVENT DATE: 29th August 2021
TIME: 11am to 12.15pm

Please reply to this message for more information.

Thanks😊
Great news👍
Very important sesions. Invitation to join today’s CUBE chatShaala,

Note: Today we are delaying the CUBE chatShaala & starting now.

Please join

Timing:6.30-9.30pm
Looking forward to your participation
Thanks.

Cardamine with developing floral bud for genetic engineering (floral dip method)dipping the floral bud in the solution containing genes.

A great achievement for our CUBE HOME LABS and CUBE CHATSHAALA as we completed a year today.
This is a histogram showing the number of participants who joined us each month from March 2020 to 18th March 2021.
How will Aditya isolate and characterize Yeast from fermented Coconut water?

Lab Assistants?
Which plates?

How the yeast colonies look?

Candida albicanshttps://en.wikipedia.org/wiki/Sabouraud_agar#/media/File:Candida_albicans_PHIL_3192_lores.jpg
Saccharomyces cerevisiae

Yeasts are eukaryotic, single-celled microorganisms classified as members of the fungus kingdom. The first yeast originated hundreds of millions of years ago, and at least 1,500 species are currently recognized.[1][2][3] They are estimated to constitute 1% of all described fungal species.[4]

Yeasts are unicellular organisms that evolved from multicellular ancestors,[5] Yeast - Wikipedia
Saccharomyces cerevisiae which is also known by its common name brewer yeast or baker yeast.
Boiled Potato slice, laced with S- shaped toddy ( fermented coconut water) trace after 2 days of incubation in room temperature on 22 July 2021 (at 15:50 hrs)), to isolate microbes that makes toddy from coconut water. CUBE Kishore Bharati HomeLab Initiative for Collaborative Science, Sophia, South Mumbai. Photo: mcarunan 22 July 2022

Will pasteurizing coconut water kill the spores also

Environmental and Genetic Determinants of Colony Morphology in Yeast Environmental and Genetic Determinants of Colony Morphology in Yeast
Baker’s yeast forms smooth round colonies when grown in favorable conditions. When starved for one or more nutrients, yeast can alter its growth pattern to produce complex structures consisting of numerous interacting cells. One mode of growth, the colony morphology response, produces visually striking, lacy colony architectures.

Uploading: WhatsApp Image 2021-08-26 at 9.18.46 PM(1).jpeg…

Why are some leaflets of Curry leaf plant showing axillary bud- like projections?

[KiranKalakotiR (Kiran Kalakoti)]

26th August 2021, 9.30pm to 27th August 2021, 9.30pm
Can someone share photographs of CUBE Lab, HBCSE, showing that even in labs we used to work with plastic bottles instead of beaker and flask !
This is for the Chai And Why?
!!!

Update on Fruit fly model
New Sub culture - SLPbA3, SLPbB3 and SLPbC3
Media Prepared on - 26/08/2021
Transferred Flies on - 27/08/2021 - 11:20am:11:30am :11:45am respectively

SLPbA3 - No of flies Transferred - 2
SLPbB3 - No of flies Transferred - ~ 30
SLPbC3 - No of flies Transferred - ~ 30
Location - Prabhadevi, Mumbai

Rechel’s CUBE Homelab, morabadi Ranchi

DATE:27/08/2/21

picture description

I Kept the setup in one of the corner of my room, currently having two model systems: Fruit fly & Cardamine
Materials visible in the picture :
(From left to right) washed bottles for fruit fly culture.
Stationeries, plastic cups, logbook, tool box ( dropper, cotton, laser light lens for microscopy, glass slide etc. inside it)
4 fruit flies culture bottles and plastic bottles.

Kept snake plant with the Home lab setup just for decoration.Can someone send good pictures of Phyllanthus plant!?
We should be able to see the hight and all in the photo.
This is for Chai and why!
@Shraddha Sonavane CUBE @Lakshmy PJ CUBE @Arunima SN Nattika Cardamine @KIRAN CUBE and others
Yes
Thank you @Janhvi Deshmukh
@Abhijeet Singh

VIDEOS SHARED BY ASWATHY AT 1.22PM
SLBK A22 (subculture of A line)…date of transfer 12.08.2021…transferred one fly.

16 days old culture bottle observed 2 flies (one is newly emerged) 2 pupae, 2 Larvae.

Update by : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu Thrissur Kerala.
SLBK B21 (subculture of B line)… date of transfer 12.08.2021…transferred 12 flies - ~5 gravid flies, ~7 male flies.

16 days old culture bottle observed ~20 flies, 50 pupae, 15 visible larvae.

Update by : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu Thrissur Kerala.

Rechel’s CUBE Homelab, morabadi, Ranchi

FRUIT FLY UPDATE
Date:27/08/2021

currently having 4 fruit fly culture bottles
RF-IIa T.D: 6/5/21. (1 fly)
RF-IIb T.D: 19/6/21 (~20 flies)
RF-IIc. TD: unknown (~50 flies)
RF-IIc". TD: unknown (~50 flies)

Good up: I didn’t mentioned the details (transfer date & media date) when I transferred into RF-IIc and RF-IIc, but I clicked the picture so that I could tag it later but didn’t find the picture in gallery.

History of the fruit fly culture

1. Trap kept on 09/03/2021 ( bait: chiku fruit peel & grapes) . Trapped 4 fruit flies on 11/03/2021.

2. Prepared TRSV medium on 11/03/2021 . I was able to transfer 2 fruit flies in the TRSV medium culture bottle (labbeled RF-II ) on 13/03/2021.

3. Media was prepared on 05/05/2021 Made subculture RF-IIa on 06/05/2021 . Transferred 4 flies from RF-II .

4. Media was prepared on 06/06/2021. Further subculture RF-IIb on 19/06/2021 . Transferred around 10 to 15 flies from RF-IIa .

5 . Further transferred fruit flies from RF-IIb to two another TRSV medium culture bottles. (Later labbeled as RF-IIc & RF-IIc" )

Further plan: to make TRSV medium to make single line cultureSLBK E17 (subculture of E line)… date of transfer 12.08.2021…transferred 14 flies - ~6 gravid flies, ~8 male flies.

16 days old culture bottle observed ~30 flies, ~60 pupae, ~20 visible larvae, ~25 dead flies.

Update by : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur Kerala.
Rechel’s CUBE Homelab, morabadi, Ranchi.

FRUIT FLY UPDATE
Date:27/08/2021
4 fruit fly culture bottles
RF-IIa T.D: 6/5/21. (1 fly)
RF-IIb T.D: 19/6/21 (~20 flies)
RF-IIc TD: unknown (~50 flies)
RF-IIc". TD: unknown (~50 flies)

Phyllanthus amarus plants grown in Panvel Home lab,location -Panvel, photo taken by- Shraddha, Date of photo-3/09/2020

ReMan’s Lab Ranchi 27-08-2021 Observing RNSL-B Fruitfly Culture Usually i click pictures in broad daylight. But when it’s a cloudy day like today, I have to do like this. Let’s find out #How_do_you_do_it? @Aswathy Suresh CUBE @Arunima SN Nattika Cardamine @Lakshmy PJ CUBE @Raj Yashwantrao Patil CUBE @Sachin Pradhan CUBE @Seethalakshmy CUBE and others…
Good idea @Man Masih Beck RANCHI CUBE.
We can follow this method
Can you taje some close up pictures by using this method?

Actually my phone does not support closeup pictures. So to overcome that i use “jugaad” technique.[4:38 PM, 8/27/2021] +91 93247 51102: Species: - Phyllanthus urinaria .
Family: Phyllanthaceae .
Identifying features:-
Habit :herb(6-15m long)
Leaf shape: oblong ( pointed leaf).
Fruit surface :- rough.
Fruit colour : - green .
Placentation : - Axil.
Number of tepals :- 6.
Male and female flowers are present.
[5:10 PM, 8/27/2021] +91 93247 51102: Fruit surface is smooth for amarus .
In amarus number of petals are 5 .
Leaf base symmetrical in amarus.
Leaf apex : obtuse (round/ slightly point

[KiranKalakotiR (Kiran Kalakoti)]

27th August 2021, 9.30pm to 28th August 2021, 9.30pm
Nematode populations
We isolated 38 new strains of C. elegans and 25 new strains of C. briggsae from single wild hermaphrodites in Kenya and South Africa in March–April 2006. Despite extensive sampling from 36 sites, Caenorhabditis nematodes were only found in four locations: (1) compost from a private garden (Johannesburg, South Africa); (2) compost from a plant nursery (Ceres, South Africa); (3) compost from a mushroom farm (Limuru, Kenya) and (4) leaf litter in a public park (Nairobi, Kenya). Strain name designations for C. elegans are ED3040–ED3077, and for C. briggsae are ED3078–ED3102. The sampling sites and strain designations are described in Table 1. Various other nematode species were also found in these sites, including many strains of Oscheius tipulae, which have also been characterized (Baïlle et al., 2008).
Nematode populations
We isolated 38 new strains of C. elegans and 25 new strains of C. briggsae from single wild hermaphrodites in Kenya and South Africa in March–April 2006. Despite extensive sampling from 36 sites, Caenorhabditis nematodes were only found in four locations: (1) compost from a private garden (Johannesburg, South Africa); (2) compost from a plant nursery (Ceres, South Africa); (3) compost from a mushroom farm (Limuru, Kenya) and (4) leaf litter in a public park (Nairobi, Kenya). Strain name designations for C. elegans are ED3040–ED3077, and for C. briggsae are ED3078–ED3102. The sampling sites and strain designations are described in Table 1. Various other nematode species were also found in these sites, including many strains of Oscheius tipulae, which have also been characterized (Baïlle et al., 2008).
https://www.nature.com/articles/6801079/figures/1

[10:12 AM, 8/28/2021] Arunan HBSCE: C elegans in Africa @Batul Pipewala Kolkata @Saida Sayyed @KIRAN CUBE
[10:15 AM, 8/28/2021] Arunan HBSCE: Which is the latest publication on Indian Cenorhabditis? @Batul Pipewala Kolkata
[11:02 AM, 8/28/2021] Arunan HBSCE: “As a model organism, the nematode Caenorhabditis elegans has provided an outstanding system for elucidating fundamental problems in biology. However, the attitude regarding the natural history and ecology of Caenorhabditis has long been ‘hakuna matata’, to use the Swahili phrase meaning ‘no worries’. To achieve a robust understanding of the evolutionary context in which C. elegans’ developmental and genetic patterns emerged, it is necessary to characterize representative natural populations on a worldwide scale and to conduct comparative analyses of Caenorhabditis species.”
https://www.nature.com/articles/6801079#:~:text=As%20a%20model,of%20Caenorhabditis%20species.
Why hunting for Indian C elegans is important?

526th Day of CUBE Chatshaala: 27th August 2021
526th Day of CUBE Chatshaala: 27thAugust 2021 – Google My Maps
16 CUBists from 16 CUBE centres had joined the webinar
INDIA
Delhi
Dr P. Chitralekha: DSC

Jharkhand
Man Masih Beck: Kokar, Ranchi
Rechel Tirkey: Morabadi, Ranchi

Kerala
Arunima: Kozhikode
Aswathy Suresh: Thriprayar
Lakshmy PJ: Kandassankadavu
Seethalakshmi CR: Pazhuvil, Trissur

Maharashtra
Abhijeet Singh: Ulwe, Navi Mumbai
Aditya Joshi: Kalyan
Arunan MC: Breach Candy, South Mumbai
Khan Shaheen: NES Ratnam College, Mumbai
Manasi …
Latest map from where C elegans has been found green dots and pink areas show where C elegans has not been found despite sampling.white areas show where sampling for C elegans has not been done

The Natural History of Model Organisms: C. elegans outside the Petri dish

[12:13 PM, 8/28/2021] Batul Pipewala Kolkata: Article by Marie Ann Felix
[12:35 PM, 8/28/2021] Batul Pipewala Kolkata: Outstanding questions about the natural history of C. elegans.

What is the pattern of C. elegans migration at different geographical distances? What are the relevant vectors? Are humans now major vectors over large scales?

Over a small scale, ongoing local population surveys will provide insights into migration patterns. Over large scales, selective sweeps estimated to date from 100–200 years ago have spread to different continents: their migration and selection might thus relate to human activity (e.g., via human travelling and the trafficking of goods, and via selection, perhaps by chemical compounds) (Andersen et al., 2012).

What is C. elegans geographical center of origin? Are there species that are very closely related to C. elegans (see phylogeny in Box 2)?

Increased sampling in poorly sampled and isolated geographical regions might provide access to divergent populations that did not undergo the recent selective sweeps and so inform us of C. elegans’ older history and habitats.

What does C. elegans eat?

C. elegans likely feeds mostly on bacteria, but we do not know which bacteria. The sterols it requires might come from yeast or from the rotting plant substrate itself.

What is the generation time and the number of generations per year in the wild?

The latter is surely far less than in the laboratory (ca. 150), but is it by one or two orders of magnitude?

What are the relevant dauer entry and exit cues?

Whether associated organisms have an influence on dauer formation has not been investigated.

Where does C. elegans spend its winter? What are the source populations?

In temperate regions, C. elegans is most often sampled in autumn, when it is found at high density. We ignore how and where this organism survives winter, for example, whether it becomes associated with a carrier organism.
Last week we had a wonderful conversation on Indian Philosophies. We will continue our barefoot chat live session-32, this afternoon from 3-4 pm at https://webinar.hbcse.tifr.res.in/b/dp6-vs2-a2s

More Details: BFC Session-32 [August 28 2021] Order and Chaos in our physical and social worlds
@Seethalakshmy CUBE, @Raj Yashwantrao Patil CUBE @Shrudhiga

VIDEO SHARED BY SANA AT 3.37PM
last year video (4/11/2020)
objective of the study:To replicate nutrient broth made in labs and inoculate microbes

Observation:A thick biofilm on home made lab
Long term objective:To grow bacteria in our home by using frugal Nutrient broth.
Home lab:Sana Jalili,Mira road
What is the frugal nutrient broth you made at home
How did you make nutrient broth in HomeLab? Please describe.
Invitation to join today’s CUBE chatShaala,
Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in all CUBE what’s app groups and on metastudio after the session

Here’s the direct link of the CUBE webinar: https://webinar.hbcse.tifr.res.in/b/kir-hye-cn4

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.

VIDEOS SHARED BY SUSANTA
Half an hour time lapse video of Sleep Wake Cycle : Phyllanthus
Date :28 August 2021
Time : 5.07 PM to 5.37 PM
Location :Sonari, Assam 27°N95°E video :Susanta Tanti

PHOTOS SHARED BY ADITIYA AT 7.20PM
Microbial colonies in less than 24 hours on boiled potato slice
GENTLE REMINDER to join tomorrow’ CHAI AND WHY? session at 11am

Date: 29th August 2021 (Sunday)
Time: 11am

Youtube link: https://youtu.be/lqUTobuDgvs

FaceBook link: Redirecting...

Looking forward to your participation
Thanks
Following are the links to spread the event.

Zoom link: Launch Meeting - Zoom

Youtube link: https://youtu.be/lqUTobuDgvs

FaceBook link: Redirecting...
All the best everyone. Rock it tmrw. Let the ripples be felt widely.
All the best to all teams!!!
Quite excited…all the best everyone…looking forward👍🏻
All the very best to Everyone and each team
Let’s have Chai and Ask Why tomorrow!
[10:22 PM, 8/28/2021] Priti Kanade: All the Best​:+1:t2:
[10:23 PM, 8/28/2021] Priti Kanade:
All the best
All the best give your best shot everyone!!

[KiranKalakotiR (Kiran Kalakoti)]

28th August 2021, 9.30pm to 29th August 2021, 9.30pm
Following are the links to spread the event.

Zoom link: Launch Meeting - Zoom

Youtube link: https://youtu.be/lqUTobuDgvs

FaceBook link: Redirecting...
All the best everyone. Rock it tmrw. Let the ripples be felt widely.
All the best to all teams!!!
Quite excited…all the best everyone…looking forward👍🏻
All the very best to Everyone and each team
Let’s have Chai and Ask Why tomorrow!
[10:22 PM, 8/28/2021] Priti Kanade: All the Best​:+1:t2:
[10:23 PM, 8/28/2021] Priti Kanade:
All the best
All the best give your best shot everyone!!
527th Day of CUBE Chatshaala: 28th August 2021
527th Day of CUBE Chatshaala: 28thAugust 2021 – Google My Maps
31 CUBists from 31 CUBE centres had joined the webinar
INDIA
Assam
Ishita Sonowal: Jorhat
Krishnendu Roy: Silchar
Susanta Tanti: Sonari

Bihar
Aryan Kumar:Sitamarhi

Delhi
Aastha Ahuja: ANDC
Dr P. Chitralekha: DSC
Raj Yashwantrao Patil: New Delhi

Jharkhand
Binita Ghosh: Ranchi
Man Masih Beck: Kokar, Ranchi
Rechel Tirkey: Morabadi, Ranchi

Kerala
Aswathy Suresh: Thriprayar
Lakshmy PJ: Kandassankadavu
Seethalakshmi CR: Pazhuvil, Trissur

Maharashtra
Abhijeet Singh: Ulwe, Navi Mumbai
Aditya Joshi: Kalyan
Arunan MC: Breach Candy, South Mumbai
Gaurangi Dhuri :Tilaknagar, Mumb…

Chai and Why? session

[image] CHAI AND WHY? SESSION We are happy to announce that we have got the chance to be the part of Chai and Why? (organized by TIFR) to share and narrate some of CUBE inventions that we came up collaboratively during the Pandemic in order to sustain research even at home during the pandemic. Chai & Why? -TIFR’s unique outreach initiative since 2009 has been the “Chai and Why?” series, a forum to enable informal discussions of interesting scientific issues outside a traditional academic set…

Click here for more info

GENTLE REMINDER to join today’ CHAI AND WHY? session at 11am

Date: 29th August 2021 (Sunday)
Time: 11am

Youtube link: https://youtu.be/lqUTobuDgvs

FaceBook link: Redirecting...

Looking forward to your participation
Thanks
We are live, it’s live now
CHEERS EVERYONE
Very well done, Saida, Man, Binita, Arunan and all the cubists.
Amazing chai and Why session.
Amazing Session​:confetti_ball:

A proud CUBIST

There may be many who may have missed the event live, do send the the youtube link widely.

Congratulations to all of us
Great collaboration
Yes
Yeah!
Session went really well…and especially without any delay!!!
Attended on youtube. Fantastic session!
Great work everyone!
Congratulations and celebrations
Thanks @Shivam CUBE NEMATODE
@Binita Cube isn’t going to like this screenshot for sure.
I enjoyed it , it was a good session , congratulations to all
Amazing work !congrats!!
Wow
Thank you @Arunan HBSCE for the kind and caring alarm ‘comment’.
@Binita Cube
What a feast
Indeed

he never miss doing that

these screenshots are inevitable.
What a feast. Mind you, it is just the beginning and surely a great beginning. Many great discoveries, indeed.
[12:47 PM, 8/29/2021] +9150803: Yeah! He is
[12:47 PM, 8/29/2021] +91 50803: Hahaha
[12:47 PM, 8/29/2021] +9150803: Oh Captain, my captain !!
You are the sail @Rahul Kushwaha
We will keep doing more chai and why sessions, next time on a specific activity.
That’s a great idea. We actually have enough flesh in all our projects. And also multiple students doing it. It’s time to develop now on individual projects as well.
Some more screenshots of Chai and why? Session
Poll results
Great collaboration…
Great show by all the enthusiastic cubits…
Excellent session Cubists…
Amazing session
Congratulations to all!

This is only the beginning!

How to make a home lab?

Please join in today’s CUBE Chatshaala to celebrate and review.

VIDEO SHARED BY MAN AT 5.39PM

Late Post Breaking News
Flies appeared after 9th day of transfer

CUBE Ranchi Re-Mans’ Home Lab
28-08-2021
Fruitfly Update

Immediate Objective: to start a single line fruitfly culture(RNSL-B) at ReMan’s Lab in TRSV Media

Short Term Objective: -To identify the trapped fruitflies
-To get native Drosophila melanogaster species

Media preparation- 18-08-2021
Transferred date- 19-08-2021

OBSERVATION
after 9 days of transfer
•Larvae ~10
•Pupae ~50
•Fly ~8 to 10

Expectation vs Reality
Earlier expectation was that New flies will emerge on 27th Aug, 2021 but they emerged on 28th Aug instead

Goof-up
I had not mentioned the time of transferring fruitflies that was on 19th Aug at around 5 pm.
@Aswathy Suresh CUBE @Yash Sheregare CUBE @Lydia cUBe Drosphila and others…

[KiranKalakotiR (Kiran Kalakoti)]

29th August 2021, 9.30pm to 30th August 2021, 9.30pm

Drosophila A Guide to Species Identification and Use

@Aswathy Suresh CUBE @Arunan HBSCE

Mango tree not started flowering!

Flowering not started
No sprouting
No Fruiting Yet.

Date : 25.08.2021,
Time : 01:47 PM

Latitude: 19.0760° N,
Longitude: 72.8777° E

Temperature :
Maximum : 25°C
Minimum: 32°C

Location: Somaiya college, Vidyavihar, Mumbai Maharashtra
CUBE Homelab Vidyavihar
Data Collector: Abhijeet Singh
Mango tree not started flowering!

Flowering not started
No sprouting
No Fruiting Yet.

Date : 27.07.2021,
Time : 05:08 PM

Latitude: 26.6438° N,
Longitude:84.9040° E

Temperature :
Maximum : 26°C
Minimum: 35°C

Location: Motihari, Bihar
CUBE Homelab Motihari
Data Collector: Arshad Shaikh
Aparna K Dinesh -
SN college Nattika
Noted. Thank you!

29th August 2021 CUBE Chatshaala - White Boards .

@Rechel Turkey Cube Ranchi Moina Please add your summary about your design of experiment, How to study Life span of moina.
Please give the location first before giving Longitude & Latitude @Abhijeet Singh

29/08/2021 Chatshala summary

In yesterday’s chatshala, we started discussing with the microscopic picture which was shared by @Navneel from Kolkata. We were trying to identify that organisms which was looking very similar to Moina and daphnia.

We also discussed classification of fruit fly and moina/daphnia so that we can differentiate between them on the basis of morphological characteristics.

@Batul Pipewala Kolkata told the story of identification of moina in CUBE by @kajal.

in Ranchi we were culturing Moina since August 2019 and lost Moina in July 2021. Which was the same well studied Moina macrocopa JSK1 which was brought by Sweta and Manjuel after August, 2019 CUBE national meet from CUBE, HBCSE, MUMBAI.

Discussed about the standard protocol for moina culture , we culture Moina in container containing 250ml dechlorinated water (DC water)and feed them 1 drop of milk everyday . We feed milk drops, so that milk bacteria will grow and moinas will feed on them.

@Soumyadip from Kolkata also told that he was also having crustaceans something like daphnia /Moina when he was culturing Azola .

Then we discussed about the life cycle and life span of Moina . So in order to check the life span we tried to set up an experimental design.

according to the hypothetical experimental design to check life span of Moina, 1st we need to identify the adult gravid Moina that can give birth to baby moinas.

Then we can isolate them in a container with 250ml DC water and feed them 1drop milk everyday day.

When the mother Moina will give birth we will get day1 moina . Then mother Moina will be separated out and we can observe day 1 moina for further observation.

When these moinas will become adult and gravid and produce baby Moina we will keep separating out new baby Moinas and we will keep observing them until they die.

                   @Arunan HBSCE @Aswathy Suresh CUBE @Batul Pipewala Kolkata @KIRAN CUBE @<U+2068>Navaneel<U+2069> @Man Masih Beck RANCHI CUBE @Janhvi Deshmukh @Saida Sayyed @Arunima SN Nattika Cardamine  and others

Added Navneel St Xavier’s College, Kolkata.
Great!
Please repost just the Design of Experiment for studying lifespan of Moina @Rechel Turkey Cube Ranchi Moina
Thank you sir.
[1:21 PM, 8/30/2021] Arunan HBSCE: Please start posting photo updates of each of our model systems. @Aswathy Suresh CUBE @Yash Sheregare CUBE @aditya and others.
[1:26 PM, 8/30/2021] Arunan HBSCE: Please write a short summary with a photo of your work @Navaneel

Present image of the organism under discussion. Data collector Navneel CUBE St Xavier’s College, Kolkata
Can see 7 baby daphnia like organisms inside @Navaneel
Hello everyone, thank you for including me in the discussion. I am Navaneel Sarangi, from St. Xavier’s College Kolkata. Project details : Experimental observations of Pondwater sample from Saltlake, Kolkata -

Objectives : Immediate Objective : To establish microscopic biodiversity of single Pondwater samples from Saltlake, Kolkata. (I have not decided whether to focus on a particular phylum)

Short term Objective : To analyse methods of enrichment of pondwater samples, as well as isolation of organisms of interest followed by culturing of the same.

Long term Objective : To establish taxonomic microbial biodiversity of pondwater samples (multiple) from Salt Lake Kolkata. Database setup with nomenclature annotation of samples visualized. Establishment of protocols for enrichment of pond water samples for microscopic analysis. Establishment of protocols for isolation of organisms of interest followed by culturing of the same.

Apart from this, I am an aspiring astrobiologist, and involved in different research activities through my position of VIsiting Scholar at Blue Marble Space Institute of Science. However, any and all aspects of science interest me, especially those we can perform without the complications of an institutional laboratory.
Micro biology and Space great combination​:ok_hand:
What is the actual size of this waterflea? @Navaneel
Hello Navaneel,

Really appreciate the detailed note of your immediate, short term and long term objectives regarding the culture and maintenance of aquatic microorganisms.
I have some questions regarding the same:

1. What do you mean by establishing microscopic biodiversity? I mean isn’t it already established in the natural source? (Salt lake) do you mean culturing?

2. What do we mean by analysis methods of enrichment of samples? A more simple elaborate sentence will be better for someone like me to understand the motive.

3. Is Salt Lake, kolkata a source of freshwater body? Why is the name salt lake ?

VIDEOS SHARED BY ASWATHY AT 3.41PM
SLBK A 22 - single line sub culture bottle of A Line… Date of transfer flies 12.08.2021.

Transfer no of flies : 1

Observations on 18th Day
30/08/2021
0 Eggs
1 Larvae (3mm)
0 Unhatched Pupae
1 Fly
1 Dead Fly

Update : Aswathy Suresh CUBE SN College Nattika.
SLBK B 21 - single line sub culture bottle of B Line… Date of transfer flies 12.08.2021.

Transfer no of flies : 12, Gravid flies : 6, Male flies : 6

Observations on 18th Day
30/08/2021
~10 Larvae (3mm)
~40 Unhatched Pupae
~20 Flies
~20 Dead Fly

Update : Aswathy Suresh CUBE SN College Nattika.
SLBK E 17 - single line sub culture bottle of E Line… Date of transfer flies 12.08.2021.

Transfer no of flies : 14, Gravid flies : 6, Male flies : 8

Observations on 18th Day
30/08/2021
~40 Larvae
~60 Unhatched Pupae
~8 Flies
~30 Dead Flies

Update : 30/08/2021 ; 3:49pm
Update by : Aswathy Suresh CUBE SN College Nattika.
What is your research project at BMSIS? CUBE is not limited you can discuss your astrobiology project here too
Please give a summary of your Single Line Cultures as BREAKING NEWS @Aswathy Suresh CUBE @Yash Sheregare CUBE @Isha Pawle @Sidhy Kerala Drosophila and others.
How many are doing good?

1. A better sentence would be to analsyse the microscopic biodiversity, and classify the same according to taxonomical heirarchy. Thank you for pointing it out.

2. Pond samples are a real-word reflection of microbial communities. These ecosystems have unqiue interactions, which control the population of each niche present. What factors help in incresing the general population of microbes in the medium ? What factors influecne particular niches ?

Eg : If we observe a community of Paramecium in our sample, can we selectively enrichen the paramecium community ?

3. SaltLake is a Municipality division in Kolkata, easily demarcated from the rest of the city. It used to be a part of the East Kolkata Wetlands, which still supports a brackish ecosystem. It was filled up in the 1950s to build one of the first planned cities of India. You can find more information here : http://www.ekwma.in/ek/wp-content/uploads/2015/09/NewsletterVolume1.pdf
   
   
   
   Location for sampling
   CHAI AND WHYCUBE presentation showed that it is possible to do research on biological research model systems at home using easily available cheap alternatives by understanding the science behind it.
   Please give your views on the presentation @Navaneel @Lakshmy PJ CUBE @Arunima SN Nattika Cardamine @Aswathy Suresh CUBE @Sana Institute Of Science @Dr Chun Mei Chang @Sunita Chahar CUBE
   What is the source of water?Is it the municipal taps?
   Yes. But I dont think it is unified with he central distribution system of saltlake

I have a question. The modified microbial culture media using coconut water, what were te constituents and standardisation of the same?

VIDEO SHARED BY SEETHALAKSHMI AT 6.17PM
𝗨𝗽𝗱𝗮𝘁𝗲 𝗼𝗻 𝗔𝗻𝘁 𝗠𝗼𝗱𝗲𝗹 𝘀𝘆𝘀𝘁𝗲𝗺

Date of photo : 30/8/21.
Time: 3:23pm.
Date of setup :25/5/21.
Location : Pazhuvil.
𝗜𝗺𝗺𝗲𝗱𝗶𝗮𝘁𝗶𝘃𝗲 𝗢𝗯𝗷𝗲𝗰𝘁𝗶𝘃𝗲 : To culture the ants.
𝗦𝗵𝗼𝗿𝘁 𝘁𝗲𝗿𝗺 𝗼𝗯𝗷𝗲𝗰𝘁𝗶𝘃𝗲 : To study it’s morphology.
𝗟𝗼𝗻𝗴 𝘁𝗲𝗿𝗺 𝗼𝗯𝗷𝗲𝗰𝘁𝗶𝘃𝗲 : How do ants communicate.

Size of ants:2.3-3 mm
[7:29 PM, 8/30/2021] Batul Pipewala Kolkata: @Aditiya Joshi CUBE please answer his query
[7:29 PM, 8/30/2021] Batul Pipewala Kolkata: Is it unfiltered water from ganga?
Navaleen: Yes I think so. It is from the older pipelines which were used to dredge

VIDEO SHARED BY SEETHALAKSHMI AT 8.34PM
Close - Up view of my culture Ant, ( By using magnifying lens.)
Feeding sugar syrup
Location: Pazhuvil
Date:30/8/21
Time:3:44pm
Data collector : Seethalakshmi
@Navaneel I used boiled potato slice as medium. I just spread the fermented coconut water on that slice.
[8:47 PM, 8/30/2021] +91 98308 28389: Can we use the coconut water as a broth for culture
[8:48 PM, 8/30/2021] +91 98308 28389: Maybe use it with rava to concote an alternative to agar ? I am not very sure of that tho.
[8:52 PM, 8/30/2021] Aditiya Joshi CUBE: Yes. I think we can.
[8:53 PM, 8/30/2021] Aditiya Joshi CUBE: Yes we can surely try it. Like in TRSV medium prepared but cubists.
[9:26 PM, 8/30/2021] +91 28389: https://www.gesundheitsindustrie-bw.de/en/article/news/the-discovery-of-homeotic-genes
[9:27 PM, 8/30/2021] +91 28389: Please check the link for homeotic genes

Update on cardamine model system.

Location : Pazhuvil.
Date:30/8/21.
Time : 4:53 pm.
Data collector : Seethalakshm

[KiranKalakotiR (Kiran Kalakoti)]

30th August 2021, 9.30pm to 31st August 2021, 9.30pm

How do you plan to study it’s morphology?
Start with objective @Seethalakshmy CUBE !
What do want to show us through this picture!?
Amazing capture @Seethalakshmy CUBE !

I think during Chai and Why? We lost the account of your identification studies.

Were are we in the identification of your ants!

Also @Gaurangi Dhuri has some similar interests as what your long term objective suggests! Please collaborate!
Chai and Why … presentations were amazing and impactful. How students have made home labs and doing research during pandemic…was so inspirational. Congratulations to everyone for the efforts and passion in science.
[11:04 PM, 8/30/2021] Seethalakshmy CUBE: Our plan is,
find immobilized ant from our culture( or catch an ant and kept in refrigerator ). Looking their body parts by using phone microscopy.
@Anjani Kumar Kashyap CUBE
[11:08 PM, 8/30/2021] Seethalakshmy CUBE: Ok @Abhijeet Singh!
my objective is To culture the Cardamine in my homelab.
Actually this is not a good culture.
Because the cardamine shows less growth as well as some plants are died.
I want to overcome this.
[11:10 PM, 8/30/2021] Seethalakshmy CUBE: Thank you!@Abhijeet Singh
Yes! you are right! We want to restart this.
[11:13 PM, 8/30/2021] Seethalakshmy CUBE: Sure! Let’s discuss collaboratively!
Yes @Gaurangi Dhuri
Please ask questions as well as tell us your ideas!
Have you noticed any specific morphological feature? Which order it belongs to?
They are coming under order Hymenoptera @Dr. Sarita kumar Delhi CUBE ma’am
Any specific character of Hymenopteran? Have you ever observed wings in ants like present in other Hymenopterans such as bees, wasps…

hello navaneel.I appreciate the work in your home lab​:tada: Keep it up!!
I am interested to know about the

1. Microbial diversity you are finding out.
   3.Why only salt lake as a source of marine ecosystem ?
2. What is your expected results.
   3.Also I would like to know the detailed observation you got from daphnia photo
   4.What is nomenclature annotation that you mentioned

@Navaneel @Batul Pipewala Kolkata @Saida Sayyed @Arunan HBSCE and others
What do you mean by immobilized ant? What benefit does that give over any non immobilized ant?
Yess…
[8:43 AM, 8/31/2021] Seethalakshmy CUBE: Yes @Dr. Sarita kumar Delhi CUBE ma’am
Hymenoptera have 2 pairs of wings (fore wings and hind wings) the fore wing is long compared to hind wing and membranous
[8:51 AM, 8/31/2021] Seethalakshmy CUBE: Immobilized means immovable / dead ant!
[8:54 AM, 8/31/2021] Seethalakshmy CUBE:

Expt documentation : Study of microbes in tap water from saltlake kolkata.
[9:40 AM, 8/31/2021] +9128389: Tap water is a common source of drinking water. In this expt, I have used coconut water (5ml ) with an ear bud full of tap water inoculum, kept in the blue lid urine collection cup. I will check for microbial growth 24 hours later. The vessel has been sealed with parafilm to prevent contamination. The urine cup seal was broken 10 mins prior to start of experiment.

To check for direct microbial growth, I envisioned potato surface based microbial growth. Here in the picture, we have a control potato slice with no inoculum on the right. The plate has been sealed with parafilm.

On the left we have the experiment potato plate. The inoculum has been streaked with an ear bud in a z pattern of continuous streaking.
[9:42 AM, 8/31/2021] +91 8389: In the absense of an autoclave, all the instruments were sterilised with home made 70% ethanol and flamed with a gas lighter. Heat sensitive material such as plastic was thoroughly cleaned with ethyl alcohol. To prevent airborne contamination, the exposure of experiment surface to air was minimised as much as possible.

Coconut water broth was collected 20 mins prior to the experiment.
Please give essential description namely Objective, Location, Date, Name of photographer along with each photo. @⁨Navnee@Navaneel
Objective : Study of microbes in Tap from Saltlake, Kolkata
Location : Saltlake, Kolkata
Date : 31/08/21
Photographer : Navaneel Sarangi
[10:03 AM, 8/31/2021] Arunan HBSCE: Plese give one or two examples from the presentation to initiate discussion @Batul Pipewala Kolkata
[10:05 AM, 8/31/2021] Arunan HBSCE: To me, it looks like gutter or drainage water, greeting stuck. @Batul Pipewala Kolkata @Navaneel
I wish it was sir. It is actually a prominent water source for the surronding market. I was very suprised to observe that
Are you @Navaneel asking about the medium used for streaking the fermented coconut water by Aditya @Aditiya Joshi CUBE?
Yes sir.
[10:11 AM, 8/31/2021] Arunan HBSCE: What is the summary of your culture method of these ants in your home lab? @Seethalakshmy CUBE
Are you recommending this method to others and are you inviting others to be collaborators? Please clarify and give a short summary from 25 May 2021 onward. Great to see this.@Seethalakshmy CUBE
[10:14 AM, 8/31/2021] Arunan HBSCE: @Seethalakshmy CUBE But you have not mentioned the name of these ants which you are culturing at your Pazhulvil HomeLab from May 2021.
Any idea which ants are these?
Common name along with vernacular name and reference to verify this, please. @Seethalakshmy CUBE
[10:15 AM, 8/31/2021] Arunan HBSCE: Please give long shot pictures of this site please @Navaneel
What type of magnifying lense? @Seethalakshmy CUBE
What is the size of these ants? @Seethalakshmy CUBE Why no reference item for measurement?
Are we not to give some reference on size?
Are we not interested to give some details about the type of ants that you are posting pictures of like whether these are all worker ants? @Seethalakshmy CUBE
Or is there a queen ant in this photo?
[10:21 AM, 8/31/2021] Arunan HBSCE: What progress did you make in the study of these ants in the last three months, from May 2021? @Seethalakshmy CUBE
[10:22 AM, 8/31/2021] Arunan HBSCE: @Aditiya Joshi CUBE A great opportunity to give photo updates with a summary since people are interested in your work @Aditiya Joshi CUBE
[10:23 AM, 8/31/2021] Arunan HBSCE: Please elaborate to continue discussion and for further development @Aditiya Joshi CUBE @Navaneel
Since you are finding microbes from tap water why are you streaking with coconut water.Another question if you make it airtight will it not increase proportion of anaerobic organisms and kill the aerobic ones?@Navaneel @Aditiya Joshi CUBE
[10:23 AM, 8/31/2021] Arunan HBSCE: Please elaborate your idea and a Design for the same @Aditiya Joshi CUBE
[10:28 AM, 8/31/2021] Arunan HBSCE: Great! @Navaneel

Please give a short description to inspire readers, to start discussing. @Navaneel

Anything interesting did you personally find, in this reference!
Please give a short description at least, as a summary with your objectives to inspire readers, to discuss further.
@Seethalakshmy CUBE
Why are we interested in Cardamine? @Seethalakshmy CUBE
How are you sure these are Cardamine? @Seethalakshmy CUBE
[10:33 AM, 8/31/2021] Arunan HBSCE: What is the culture condition? @Seethalakshmy CUBE
Why so few seedlings?
What is your objective?
Let’s inspire others to collaborate, by giving essential information.
[10:35 AM, 8/31/2021] Arunan HBSCE: Absolutely @Abhijeet Singh
Please give us the short story, starting with your objective @Seethalakshmy CUBE
[10:37 AM, 8/31/2021] Arunan HBSCE: What is the purpose? @Seethalakshmy CUBE
Are all ants from the colony be alike?
[10:38 AM, 8/31/2021] Arunan HBSCE: Please give the details @Seethalakshmy CUBE
Let’s all join after knowing your objectives.
[10:39 AM, 8/31/2021] Arunan HBSCE: Any reference and any further reasons to believe that these are hymenopterans? @Seethalakshmy CUBE
[10:42 AM, 8/31/2021] Arunan HBSCE: Reference please. @Seethalakshmy CUBE

Quarantine Mood Sparing breakfast(Upma) with fruit flies . Successfully trapped two fruit flies (one is gravid) today in the morning at 7am. Date of photo: 31st August 2021
[10:47 AM, 8/31/2021] Arunan HBSCE: Please include objective in each post like this @Navaneel
Any BREAKING NEWS from your Salt Lake HomeLab?
[10:50 AM, 8/31/2021] Arunan HBSCE: What do you mean by prominent water source? Do people use the water retained in the tank for daily use?

Or, people use the running water from the tube into the drainage looking pit for drinking etc. Please clarify.
[10:51 AM, 8/31/2021] Arunan HBSCE: What is the exact source of your water for water fleas and paramecium etc., shown in some earlier pictures? @Navaneel
[10:52 AM, 8/31/2021] Arunan HBSCE: Please clarify to continue discussion with @Aditiya Joshi CUBE and others. @Navaneel
[10:58 AM, 8/31/2021] Arunan HBSCE: GREAT to see HomeLab at Homi Bhabha Center🤣 Almost scandalous😌 @KIRAN CUBE
Looking forward to your Quarantine Labwork @KIRAN CUBE

Please repost the photo with corrections.
[11:11 AM, 8/31/2021] +91 28389: ok sir
[11:11 AM, 8/31/2021] +91 28389: Yes sir we are
[11:12 AM, 8/31/2021] +91 28389: Not streaking with coconut water. I am using coconute water as a broth to culture any microbe in the tap water. If the quantity of microbes is less, then we may not get any colonies on the potato, but the coconut water may enrichen the same
[11:15 AM, 8/31/2021] +91 28389: For short term growth like 24 hours, my previous laboratory experience has been that there is no drop in optimum growth of culture for aerobic microbes. But as you mentioned, I may do a comparative study of sealing them and not sealing them to see if there are any changes.

[11:22 AM, 8/31/2021] Arunan HBSCE: Please be careful because you are possibly enriching microbes from elsewhere​:face_with_monocle:, like from tap water using nutrient rich coconut water. @Navaneel
What are your EXPECTATIONS?

Please explain your DESIGN fully to be aware of possible consequences.
[11:25 AM, 8/31/2021] Arunan HBSCE: Don’t you expect any pathogenic bacteria being enriched in this process?
Let’s write down the full Design clearly and discuss the consequences. @Navaneel

Do you see a difference between that of @Aditiya Joshi CUBE and of your Design of HomeGrown Microbes? @Navaneel
Design of the experiment :

an earbud soaked in tap water is immersed into 5ml of Coconut water. The experiment is being carried out in a safe enclosed environment, adjacent to a doctor’s chamber.

Expectations : There should be no microbes present in the tap water submerged culture as it is chlorianted by the Municipality. Likewise, the surface culture on potato should not yeild any bacterial isolates.

Alternate Hypothesis : If there is presence of colonies of microbes on submerged or surface culture, there is contamination of microbes in the tap water being used for drinking resources.

Since most of us use this water in one way or the other without additional filtering, and there have been no untoward incident so far, I expect the microbes been enrichened are not pathogenic.

Opening and closing of leafs pattern observed in Chini Gulab or Sun Rose . Botanical name:- Portulaca grandiflora My mother brought this plant from nearby nursery two weeks before. I observed the leafs showing some closing and opening kind of pattern. So to look into it I took a photo yesterday in the evening and a photo this morning. I can clearly see that the plant’s leafs are opening and closing. Compare the two photos. What do you see @Lakshmy PJ CUBE @Arunima SN Nattika Cardamine @Aswathy Suresh CUBE @Arunan HBSCE @Batul Pipewala Kolkata @Aditiya Joshi CUBE . Can we also developed Chini gulab as a model for sleep wake studies in Phyllanthus??? Please post your comment and help me do this! Looking forward…!Yes sir. In case Aditya’s experiment design, the microbes being enrichened are directly derived from the fermented coconut broth. Since the coconut broth is usually consumed in some manner or the other, the organisms are not pathogenic.

In my case, I am not using the coconut broth itself as the isolate, but as a medium for enrichment.
How does Doctors’ chamber help?
The doctor’s chamber provides a sterile environment to work in and has air flow systems isolated from any living area. All air inlets are fitted with HEPA filters. I myself use a lab coat, sterile nitrile gloces and mask while processing any sample. I make sure to sterilsie all and any surface with 70% ethanol before and after the experiment.
[11:31 AM, 8/31/2021] Arunan HBSCE: Why do you doubt? @Abhijeet Singh
Time in AM & PM is essential for getting clear idea.
Please give essential details
[11:34 AM, 8/31/2021] Arunan HBSCE: Do you expect to get collaborators, if your initial requirements are this stringent? Can’t we do microbial research without such demanding requirements? @Navaneel
[11:35 AM, 8/31/2021] +9128389: yes sir, that is some additional precaution. Tap water shouldnt have any pathogenic microflora, and rhizobial cultures can also be processed without any of the above requirements. In fact i used to do the same, but due to the worry of my parents (who are doctors) I had to move to the same area
[11:35 AM, 8/31/2021] +91 28389: However, the requirement of gloves and ethanol or any sanitiser must be there
Why do you need coconut water to enrich them will not boiled potato itself provide nutrients to the microbes?
They are two seperated mediums mam. One is a submerged culture medium (Coconut water) and the other is a surface culture medium. Often, especially in the laboratory, we first perform a submerged culture to increase the concentration of microbial cells before following through with a surface culture.
Let’s move forward step by step. @Navaneel
What is your research question?
Let’s clarify and proceed.
Research Question : Are there any microbes coming with the municipality tap water
Details:-
Time of pic on left side :- 8.35 AM
Time of pic on right side:- 5.40PM

Place:- Ulwe,Navi-Mumbai.

This is a boiled potato slice showing growth of bacteria from just air after 24hrs@@Navaneel @Aditiya Joshi CUBE
yes that is why i had to seal the potato in. Otherwise there would have been air borne contamination
Correction @Navaneel On this boiled potato slice I had transferred nematodes with two drops of tap water.Nematodes also have bacteria attached to their bodies and tap water bacteria are also present besides air.
[11:58 AM, 8/31/2021] Batul Pipewala Kolkata: Sorry there is a mistake ,Correction given above
[12:02 PM, 8/31/2021] Batul Pipewala Kolkata: For airborne contamination you can put a control slice exposed to air. What do you think @Aditiya Joshi CUBE @Navaneel is the importance of controls in an experiment
[12:02 PM, 8/31/2021] Arunan HBSCE: Please post your Control simultaneously. @Batul Pipewala Kolkata
What were the Controls?
Are they not crucial?@Batul Pipewala Kolkata @Navaneel
Let’s go ste by step.
[12:03 PM, 8/31/2021] Arunan HBSCE: Let’s pay more attention to Design of experiment based on what is our Hypothesis that we are testing. @Batul Pipewala Kolkata @Navaneel
Let’s state the Hypothesis.
Yes, my control is on the side of the expt potato. It has every single exposure except to tap water. Any bacterial growth on that potato would be independt of tap water
[12:03 PM, 8/31/2021] Batul Pipewala Kolkata: Yes Sir controls will give a clear pic by comparison with the test
[12:04 PM, 8/31/2021] Batul Pipewala Kolkata: Can you show in the pic label the control
Just one potato slice or in at least duplicate?
Let’s have a full DESIGN, stated clearly. @Navaneel @Batul Pipewala Kolkata @Aditiya Joshi CUBE
Atleast 3 potato slices as test replicates would be needed besides the controls @Navaneel
yes it is labelled mam. I am sendin the experiemnt design none the less

Mango tree started flowering!

Flowering Started
No sprouting
No Fruiting Yet

Location: Kovilpatti, Tamilnadu
CUBE Homelab Kovilpatti

Date : 24/08/21
Time : 7.21AM

Latitude: 9.1727° N,
Longitude:77.8715° E

Temperature :
Maximum : 26°C
Minimum: 35°C

Data Collector: Chitra Achari Trying to recieve more photos from Kovilpatti.
Also clarify which test slices for submerged and surface culture?

30th August 2021 - CUBE Chatshaala Discussion - White Boards

Discussed about model systems : Fruit Fly, Mosquito, Ants (Insects).
30th August 2021 : Discussion white board
Where can we find Fruit Flies?

How Fruit flies Look Like? Is it easy to distinguish from other insects?

What are the characteristics features that very well visible in fruit flies? Which part of the body well visible like… Wings, Eyes, Abdomen…? What is the actual size of this small flies?
What are similarities & Dissimilarities between Fruit flies and Mosquitoes?

Fruit Flies and Mosquitoes, both of them are belonging to;

Kingdom : Animalia

Phylum : Arthropoda (Because both of them have joined appendages)

Class : Insecta (Body divided in to 3 parts Head, Thorax abdomen. Have 3 pair of legs & 1 pair of antenna, also have compound eyes.)

Order : Diptera
Both have 2 wing (one pair of wing)

Coming in to Family level Fruit Files are belonging to Drosophildae, Mosquitoes are belonging in to Culicidae.
One interesting point added by @Navaneel that insects have fore wing & hind wings. Only Fore wing is Expressed. Hind wing converted in to halteres in the case of Dipterans.

Flies are insects of the order Diptera, the name being derived from the Greek δι- di- “two”, and πτερόν pteron “wing”. Insects of this order use only a single pair of wings to fly, the hindwings having evolved into advanced mechanosensory organs known as halteres, which act as high-speed sensors of rotational movement and allow dipterans to perform advanced aerobatics. Fly - Wikipedia

Reference given by @Yash

But did other insects have halteres first?

Are there examples of insects having just halters…?

It is generally accepted that the halteres evolved from…
So if it is easy enough for you to keep an ant in the refrigerator and immobilize it have you obtained any immobilized ants yet?
Wow! Even before September?
Please get what percentage of Mango trees at this lower Latitude are flowering before September? @Abhijeet Singh
@Aswathy Suresh CUBE
intresting mam …
How do we really know what we got is Fruitfly and likely to be Drosophila? @Aswathy Suresh CUBE @Yash Sheregare CUBE @Raj Yashwantrao Patil CUBE @Aastha Ahuja Cube
Yes sir!
I trying to receive more data from this place and state! Crucial time in mapping these observations!

Home lab at Homi Bhabha Center

Quarantine Mode
Trapped three fruit flies in two bottles.

Seems to be of different types of fruit flies.
Can we standardise the angle at which we are taking the photos? Then maybe it can be analysed better. Angle as in the lateral view, or the ventral view.
Wow… Great what is your further plan @KIRAN CUBE ?
Plan is to start the single line Cultures of these fruit flies and identify them.

After getting 1st progeny of these fruit flies I will be transferring in medium.

Expecting to see eggs tomorrow (1st September 2021)

VIDEOS SHARED BY YASH AT 4.03PM

Updates on Fruit fly cultures
Location - Prabhadevi, Mumbai

SLPbA3
Highlight - On 4th day from transferring 3 fruit flies, there are no larvae seen today.

Media date - 26/08/2021
Transfer date - 27/08/2021
No of flies transferred on 27/08/2021 - 3

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality

31/08/2021
No of flies - 3
No of larvae - 0
No of pupae - 0
No of dead flies - 0

Expectations
31/08/2021 - more larvae and pupae - ~ 20
1/09/2021 - More pupae - 30
4/09/2021 Newly hatched fruit flies @Arunan HBSCE sir @Lydia cUBe Drosphila @Aswathy Suresh CUBE @Isha Pawle @Sidhy Kerala Drosophila @Janhvi Deshmukh
Updates on Fruit fly cultures
Location - Prabhadevi, Mumbai
SLPbB3
Highlights - SLPbB3 shows 5 pupae and a few larvae on 4th day of transfer of fruit flies

Media date* - 26/08/2021
Transfer date - 27/08/2021
No of flies transferred on 27/08/2021 - 30

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality

31/08/2021
No of flies - 28
No of larvae - 6- 3rd instar larvae
No of pupae - 5
No of dead flies - 2 @Arunan HBSCE sir @Aswathy Suresh CUBE @Isha Pawle @Sidhy Kerala Drosophila @Lydia cUBe Drosphila and others
Updates on Fruit fly cultures
Location - Prabhadevi, Mumbai
SLPbC3
Highlights - Only 2 larvae seen on 4th day of sub culture on 27/08/2021

Media date* - 26/08/2021
Transfer date - 27/08/2021
No of flies transferred on 27/08/2021 - 35

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality

31/08/2021
No of flies - 33
No of larvae - 2- 3mm larvae
No of pupae* - 0
No of dead flies - 2

Expectations
31/08/2021 - Pupae and larvae - 10-20
*1/09/2021 - More pupae - 20+
4/09/2021 - Newly hatched fruit flies @Arunan HBSCE sir @Aswathy Suresh CUBE @Isha Pawle @Lydia cUBe Drosphila @Sidhy Kerala Drosophila @Man Masih Beck RANCHI CUBE and others
Goof UP - Ignorance is not a Bliss - SLPbA2 culture flies shows a Different fly other than Fruit fly!. (suspect it to be a Phorid fly)
Media is liquidy!
I didn’t check this bottle for almost a week and couldn’t transfer the fruit flies that were present.
Today’s Headline - 24th day of Culture - SLPbA2 shows no fruit flies but a Different fly - suspect it to be Phorid fly!

Media date - 06/08/2021
Transfer date - 7/08/2021
No of flies transferred on 7/08/2021 - 35

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality

31/08/2021
No of flies - 1 (suspecting a Phorid fly)
No of larvae - 0,
No of pupae - 0
No of dead flies - 30

Immediate work done to save SLPbA line - 1) Made TRS-V media on 26/08/2021 and Transfer the Fruit flies 3 alive in New Media Bottle on 27/08/ 2021
2) Keep the SLPbA2 bottle separate so that it there is no chance of this fly contaminating other cultures
Today’s Headline - 24th day of Culture - SLPbB2 shows 20 fruit flies.

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality

Media date - 06/08/2021
Transfer date - 7/08/2021
No of flies transferred - 30

31/08/2021
No of flies - 20
No of larvae - 2- 3mm larvae
No of pupae - 0
No of dead flies - 20
[4:36 PM, 8/31/2021] +91 87289: Where was this bottle kept ?
[4:39 PM, 8/31/2021] +9187289: Are this kind of flies usually seen in the place … where you catch fruitflies?
What are these brown patches in the bottle? Are these pupae?

Can we also highlight the objective to map Drosophila melanogaster from different parts of the India that’s Geographical distribution of Drosophila melanogaster across the India by the CUBist.

Aswathy
Sidhy
Yash
Man Masih
Isha
(Please add names)

We are currently 5 people to have single line cultures of fruit flies and I have also initiated hopefully get 2st progeny, can we also take the initiative and find out which species are these?
Great! @KIRAN CUBE maam the second bottle on right shows an interesting fly trapped…
Almost looks like the bottom fly has black eyes
Are they fruit flies?
Let’s find out in somedays
Or make a guess
82 pupaes! That’s a huge number in a few days there would be plenty of fruit flies @Man Masih Beck RANCHI CUBE @Aswathy Suresh CUBE @Isha Pawle

CUBE Ranchi Re-Mans’ Home Lab
31-08-2021
Fruitfly Update

Immediate Objective: to start a single line fruitfly culture(RNSL-B) line at ReMan’s Lab on TRSV Media

Short Term Objective: -To identify the trapped fruitflies
-To find native Drosophila melanogaster species from Ranchi

Media preparation- 18-08-2021
Transferred date- 19-08-2021

OBSERVATION
after 12 days of transfer
•Larvae ~7
•Pupae ~82
•Fly ~ 12 to 15

Highlights
•flies first appeared on 28th Aug(9th day after transfer)
•most larvae have turned into pupae
[5:11 PM, 8/31/2021] Yash Sheregare CUBE: Today’s Headline - 24th day of Culture - SLPbC2 shows 10 fruit flies.

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality

Media date - 06/08/2021
Transfer date - 7/08/2021
No of flies transferred - 30

23/08/2021
No of flies - 10
No of larvae* - 0
No of pupae -
No of dead flies - 25
@Arunan HBSCE sir @Aswathy Suresh CUBE @Isha Pawle @Lydia cUBe Drosphila @
[5:11 PM, 8/31/2021] Yash Sheregare CUBE: These bottle is kept near doorway beside bed…

Goof up
I am guessing the cotton plug wasn’t tight enough and that invited a Different fly inside the Single line culture - a culture that was started with just 1 pregnat fruit fly that gives a pure culture
@Gaurangi Dhuri
The brownish and some what blackish patches are the empty pupal cases
Objective - To Culture and Map and find Drosophila melanogaster in our Locality and Entire India through Collaboration by CUBists
@Batul Pipewala Kolkata mam and @Aditiya Joshi CUBE I had a question. Are you using normal potato or boiled potato for the substrate?
For nematodes I am using raw potato @Aditiya Joshi CUBE is using boiled potato as a substrate for microbiological work
Ooh …

(upload://20zldg4skVENmrMPN7IKjIdbWmP.mp4)
Movement of fruit fly. Home lab HBCSE.
Has taken 12 days to pupate 82 larvae. Is it a huge number? @Aswathy Suresh CUBE @Sidhy Kerala Drosophila @Isha Pawle @Yash Sheregare CUBE @Rechel Turkey Cube Ranchi Moina and others…
![WhatsApp Video 2021-08-31 at 6.10.02 PM(1)|video]

[KiranKalakotiR (Kiran Kalakoti)]

31st August 2021, 9.30pm to 1st September 2021, 9.30pm
Has taken 12 days to pupate 82 larvae. Is it a huge number? @Aswathy Suresh CUBE @Sidhy Kerala Drosophila @Isha Pawle @Yash Sheregare CUBE @Rechel Turkey Cube Ranchi Moina and others…

Caught some mosquitoes at home(Ulwe-Navi mumbai)
Objective:- Continuing Prediction and prevention of mosquito born diseases(dengue, chickungunya) Project.
History of Mosquito project:-
I started mosquito project when I was in 7th grade(2015-16) with my school friends(Adarsha Vidyalaya, Tilak Nagar, Mumbai) and collaborators from Deonar public school(Govandi, Mumbai). Including @gaurangi. We together used to catch mosquitoes at school and individually at our homes. After we catch the mosquitoes we place them on a black background and look for mosquitoes with white stripes(easily visible on black background). The mosquito with white stripes are Aedes mosquito and they are responsible for spreading diseases like Aedes and Chickungunya. So we mainly look for these mosquito. Did this for around one year or two and found out that Aedes mosquitoes are found highest in August.

So now let’s see if we get any Aedes mosquitoes from starting of September in Ulwe, Navi-Mumbai.
Today (31st Aug 2021) I got 10 mosquitoes.
No Aedes mosquitoes.

Looking forward for collaboration. Aug 2021) I got 10 mosquitoes.
No Aedes mosquitoes.

Looking forward for collaboration and comments from @Gaurangi Dhuri @Hina Mudgal Kanpur CUBE @Aswathy Suresh CUBE @KIRAN CUBE @Arunan HBSCE @Batul Pipewala Kolkata
Aedes is not a disease… it’s a type of mosquito *
@Hina Mudgal Kanpur CUBE what’s the update on your mosquito studies!? Please share the updates on your work!
Yeah… I would love to collaborate with you… and restart our old project
@Abhijeet Singh @Gaurangi Dhuri
[11:12 PM, 8/31/2021] Abhijeet Singh: Sorry for that! It was a mistake!
[11:12 PM, 8/31/2021] Abhijeet Singh: Looking forward…!
No problem… I shall also restart with mosquito then…
Let’s plan accordingly! What should be our immediate objective @Gaurangi Dhuri !
Also short term and long term?
Let’s built it up based on our past experience. Help me recall too!!
That time we had two hypotheses… of the most no. Of mosquitoes all over the year… and avt aedes mosquitoes when found most…

VIDEOS SHARED BY ASWATHY AT 12.40AM
Fruitfly Update

BREAKING NEWS : 58 Days Old ‘E’ Culture Bottle Still Have Flies,Larvae,Pupae.

SLBK E15
Transfer Date : 02.07.2021
Transfer Flies : ~20
Gravid : ~8, Males : ~12

Objective : To find D.melanogaster from my native area (Bottukadavu - Thrissur Kerala) Want to compare with St. D.melanogaster.

31/08/2021
(observation today)

No of Flies : ~8
No of Larvae : ~9
No of Pupae : ~10
No of Dead Flies : ~50
Media Conditions : Slightly liquefied.

Update on : 31/08/2021
Location : Bottukadavu - Thrissur Kerala.
Microworms

43 Days Old Fruitfly Culture Bottles ; Microworms Started Presenting Inside Bottle Wall.

SLBK E16
Transfer Date : 19.07.2021

Objective : To Identify This Worm (Is it Nematodes or some other parasite.)

31/08/2021
(date of observation)

Very few number of microworms observed inside the culture bottle. Fruit flies, Larvae, Pupae also present inside the culture bottle. Media solidified conditions.

In order to identify microworms before need to culture them separately.

Immediate Objective : To make media (TRSV) to culture microworms separately to identify them.

Breaking News . Observed approx 20 Larvae in A bottle that’s post 24 hours of trapping fruit flies.

Observed single egg in the B bottle after 17 hours trapping of fruit fly. Date of photo: 1st September 2021, 8amA great beginning again @Abhijeet Singh after a long break during 10th Grade till 12th Grade.
But, don’t you think the lighting is too much that we cannot see the image as expected?
What should be the lighting conditions for getting a picture that can show clearly black and white stripes? @Abhijeet Singh @Gaurangi Dhuri @Hina Mudgal Kanpur CUBE @Aswathy Suresh CUBE

Caught 5 mosquitoes yesterday night at 11.45 pm 0 aedes found as tried identifying on black background…How to identify Aedes @Gaurangi Dhuri
The image is not clear to get evidence that none of them are Aedes mosquito @Abhijeet Singh
How do we resolve it?

What is the minimum quality of an image, for achieving the objective? @Abhijeet Singh @KIRAN CUBE @Saida Sayyed @Aswathy Suresh CUBE @Aditiya Joshi CUBE @Man Masih Beck RANCHI CUBE and others.
If the body of the mosquitoes have white stripes present on it including the legs region… then it’s an aedes mosquito
Great! @Gaurangi Dhuri
Which college are you presently in? Let’s start a CUBE group with your friends and their relatives for CUBE HomeLabs. @Gaurangi Dhuri
[9:07 AM, 9/1/2021] +91 88988 87289: Maybe the sorrounding high power of light from the top should be avoided…
[9:08 AM, 9/1/2021] +91 88988 87289: I am in V.G.Kelkar college… yes sir… I have thought of introducing CUBE to our college’s research grp
From white stripes we confirm its a aedes ? @Gaurangi Dhuri
Yess
Any another features ? @Gaurangi Dhuri
Please highlight the mistake @Abhijeet Singh Let’s celebrate the Goof-ups, big and small. Let’s have Confessions of Scientists!
There are other characteristics like… the sitting position and all… but for these characteristics to be used for identification… we need to keep observing the mosquitoes when they are alive … But we can easily see the white stripes with our naked eyes when we kill the mosquito
[9:16 AM, 9/1/2021] +928389: In continuation to this
[9:19 AM, 9/1/2021] +928389: Breaking new from Navaneel’s home lab on the analysis of tap water samples.

Before breaking the seal, this is the 24 hour observation of te experimental potato substrate streaked with tap water sample. There are no apparent contamination, or growth of bacterial colony isolates on the surface of the potato sample.

This is the 24 hour observation of the control sample. It correlates that there was no contamination of the sample there are no apparent growth of bacterial colony isolates on the surface of the potato.

This is 24 hour observation of submerged culture of tap water in coconut water. Due to the natural milkiness of coconut water, it is difficult to say if there is any bacterial growth. However, I made a mistake of not keeping a control in this case which makes differentiating difficult. This needs to be noted I exp design.[9:29 AM, 9/1/2021] +91 98308 28389: Possible Avenue of reason for no growth of colonies on expt potato ;

1. There is no bacterial contamination in tap water
2. The contamination is an obligate aerobe, an since I sealed the petri dish, it couldnt grow optimally. Due to this, I have to envision some control to remove this conclusion.
3. The contamination in water sample is a slow grower and does not culture in 24 hours
4. The contamination does not find adequate growth medium in potato based substrate.
   5 @Aditiya Joshi CUBE used boiled potatoes, which degrades some complex material. I used raw potato. This may have lead to difficulty in the bacterial growth.
   [9:31 AM, 9/1/2021] +91
   28389: Next step of experiment: to debug all of the above problems, so that only 1 is the reasonable answer. I am hopeful of collaboration in that aspect.

To debug problem 5, I will use tap water and culture it on boiled potatoes and seal it.

To debug problem 3, I am going to leave my present potato samples for 24 hours more.

2. To debug this step, I am going to use tap water to culture on both raw and boiled potato but without sealing off the petri dish
   [9:32 AM, 9/1/2021] +91 928389: In case of number 4, I do not have an answer yet
   [9:32 AM, 9/1/2021] +928389: Due to the good up in the submerged culture, I am going to redo the step once more with fresh medium and control.
   [9:33 AM, 9/1/2021] +91 28389: Te present culture, just for the sake of it, I am going to plate it on potato surface medium
   [9:38 AM, 9/1/2021] +91 28389: Objective : analysis of tap water samples
   Date : 1/09/21
   Data collector : Navaneel Sarangi
   
   
   
   Objective : to study bacterial growth from coconut water medium after 24 hour culturing period on boiled potato medium with aerobic respiration
   Date 1/09/21
   Image : Navaneel Sarangi
   Location Saltlake kolkata
   
   
   Control slice
   Is “Rava” a source of nutrient for Microbes?

Is the discussion about developing an alternative Homemade Nutrient Broth like the way Fruitfly Collaborators developed an alternative of Cornmeal Media? @Aditiya Joshi CUBE @Navaneel and others…
[10:00 AM, 9/1/2021] +91 28389: Yes.
[10:00 AM, 9/1/2021] +91

Breaking news… I got 10 mosquitoes in just around 5-10 mins in the bathroom… at 10am when I was taking a bath… But yesterday night when I checked in the bathroom, I was unable to find a single mosquito…But I made a goof-up that… Many of the mosquito body parts got damaged … I think that happened as I caught the mosquitoes with wet hand and kept them when on a damp place due to which while picking them … many parts of mosquitoes break apart.
Please post some photos @Gaurangi Dhuri @Janhvi Deshmukh
Better lighting will help.@Navaneel
Yes sir, I also think that😅.
I think the problem is not lighting but focus of my camera. I’ll try to clear image next time!

What do you think @Gaurangi Dhuri @Aswathy Suresh CUBE ?
I think it’s not just focus of camera… but the background light from the top have affected the photo … due to which we are unable to see the mosquitoes clearly as in the white stripes
Yes sir I got your point!
So as we are looking for Aedes mosquito which has white stripes on its legs we need to have a clear photo to show the evidence to everyone that yes none of them is Aedes.

I think the minimum quality of an image should be that the should be clear in the sense of what are we trying to show through it!

So as we want yo show that none of the mosquito are Aedes(white striped once) the Focusing and all of the camera should done as per that!

Please correct me if I’m wrong @Arunan HBSCE and others!
Don’t you see some whitish patches?
How about giving essential information like Objective, Date, Location of HomeLab, Name of Data collector with every photo for readers to get the Context. @Navaneel

I think this image would help us to understand that how Aedes mosquito looks like! So we can clearly see the white stripes on the legs and body of the mosquito . By which we identify that yes it’s a Aedes mosquito. @Janhvi Deshmukh @Gaurangi Dhuri
Please give essential information like Objective, Date, Location of HomeLab, Name of Data collector with every photo for readers to get the Context. @Navaneel
Reference :- Wikipedia

Aedes

Yeahh … this can surely help us to know… how exactly the aedes mosquitoes look like
What is your Hypothesis? @Navaneel Please state the Hypothesis.

These are some of my aedes mosquitoes … this is an old photo … In this the white stripes are visible… If u check closely
Any explanation why the potato slice is already bot looking intact? @Navaneel
What should be the ideal design to test your Hypothesis? @Navaneel
Sir the boiled potato is inherently very unstable sir . That lead to problems in generating proper slices.

I am writing the entire expt in words, so that is is easier to read and in one place. I will send it in by today.
Please describe in full, short sentences @Navaneel so that a newcomer too can follow it.
The four mosquitoes which are at the bottom we can clearly see white stripes… and even in the top one… but in this picture also… due to some light issues… all the mosquitoes are not clearly visible to check white stripes
How about posting better photos showing lighting, focus and contrast.
How to improve the photography? @Gaurangi Dhuri
Yes sir … we need to sort out with the photography … so that we can clearly check whether the white stripes are present from photos
Yes sir!!!
So actually in earlier post I did a typing mistake that:-
Aedes mosquito are responsible for spreading diseases like ’ Aedes ’ and Chickungunya.

So the the words Aedes that I have strikedthrough was a mistake and it was conveying that Aedes is a kind of Disease.

Correction:-
Aedes mosquito are responsible for spreading diseases like Dengue, Chickungunya.
[10:31 AM, 9/1/2021] Arunan HBSCE: Which parts of mosquitoes are important for achieving your Objective?
Please always start with Objective and also give Location of CUBE HomeLab, Date, Name of Data Collector with each photo @Gaurangi Dhuri
[10:32 AM, 9/1/2021] Arunan HBSCE: What is your comment on the bathroom mosquito catch by @Gaurangi Dhuri ? @Abhijeet Singh
I think … I saw many mosquitoes in the bathroom today… because in the night they maybe coming out in other rooms for food and in morning they maybe coming to their original position… maybe they are breeding in bathroom itself…
[10:35 AM, 9/1/2021] Arunan HBSCE: So what is your plan to trouble-shoot this issue of taking photos? @Abhijeet Singh
Please comment on photos of @Navaneel
Let’s improve the quality of data while posting.
Let’s have a discussion to resolve this urgently and publish a Blog on it in Stem Games.
[10:37 AM, 9/1/2021] Arunan HBSCE: What is our Long term Objective by collecting Mosquito data? @Gaurangi Dhuri @Abhijeet Singh @Hina Mudgal Kanpur CUBE
Are they all fruitfly larvae?
[10:40 AM, 9/1/2021] +91 87289: Okk … so our basic objective to catch mosquitoes is to find in which month in the whole year are we getting more mosquitoes and in which month are we getting more aedes mosquitoes… so to identify aedes mosquitoes… we need to find whether the legs and stomach of mosquitoes are having white stripes… If there are white stripes present in the legs then it’s an aedes mosquito… so to identify this… we need to take care that … the legs are not damaged
[10:43 AM, 9/1/2021] +9187289: Our long term objective is to find the most amount of mosquitoes in which seasons… and most no. Of mosquitoes in which season… so accordingly we can take more precautions in particular season… and even inform others
[10:43 AM, 9/1/2021] +91 87289: Am I right @Abhijeet Singh @Hina Mudgal Kanpur CUBE … ??
What are the white spots in the pic are they not bacterial colonies @Navaneel
[10:47 AM, 9/1/2021] +9128389: Mam i think they are instrinsic to the potato
[10:47 AM, 9/1/2021] +91 8389: those are starchy exudates which I was observing. Quite distinct from bacterial mucilaginous colonies.
[10:48 AM, 9/1/2021] +9128389: But I can be wrong in identification, I will break isolate tomorrow to allow for debuggin the long term growth problems28389: for plating in general

Are u talking About this white spots @Navaneel … ??Did you see them before plating? Any pic evidence?
[10:48 AM, 9/1/2021] Batul Pipewala Kolkata: Yed
[10:49 AM, 9/1/2021] Batul Pipewala Kolkata: Yes
ah no. those are water vapour marks
[10:49 AM, 9/1/2021] +91 87289: Yaa that’s what I thought
[10:49 AM, 9/1/2021] +91 87289: Hence asked
I will click a better picture
Okk
Ok

[10:50 AM, 9/1/2021] +91 28389: This was the start of the experiment
[10:50 AM, 9/1/2021] +9128389: I should have clicked a closer picture
What was your expectations in each case @Navaneel !?
I think with the objective, expectations are also an important aspect!!
[11:37 AM, 9/1/2021] +91 28389: .
[11:37 AM, 9/1/2021] +91 28389: @Abhijeet Singh
[11:45 AM, 9/1/2021] Abhijeet Singh: I think you mean to say ‘goof up’.
So as you are going to redo the submerged culture, what will do do with the submerged culture you have currently!?
I think we should also follow up that! Let’s see what happens!!
That’s would be great @Navaneel !! Please send us updates!
Also when you redo the submerged experiment let’s think of what are you going to name it! Because the labels of the new one should not let us forget the one which you have currently let’s together follow it up!
@Navaneel @Aditiya Joshi CUBE @Arunan HBSCE @Aswathy Suresh CUBE
[11:51 AM, 9/1/2021] Abhijeet Singh: I think the legs are very important for differentiating between Aedes and non-aedes mosquitoes. So while trapping lets make sure that we don’t hit that hard that it’s legs gets broken from its body!
What do you think @Gaurangi Dhuri !?
Yess… I also wrote the same thing… that legs plays an important role in indentification
The photos which she sent needs to have a better resolution!
And gaurangi and I discussed about catching mosquitoes in bathroom. So actually at my place (ulwe) mosquitoes come in evening by 5.45 pm or something like that.
At day time we can see that. When we do cleaning and all in the morning they are found behind the table or underneath the bed. As in the dark places!
And better lighting and better focus too.
Please start clicking and posting better evidences. @Navaneel
yes sir
Now I’m not able to make it in a statement but let’s try to construct it through what we understand.

So what I think our long term object should be is that we should be to able to predict the diseases that may occur in a month or months particularly as per the data we collect through mosquitoes Trapping and identification of mosquitoes trapped

I don’t know if the idea is transferred but let’s discuss!!
@Gaurangi Dhuri @Arunan HBSCE @Hina Mudgal Kanpur CUBE
What do you mean by submerged experiment?! @Abhijeet Singh @Navaneel
What is the objective?
What is the research question?
Yes, what is your expectation based on your stated Hypothesis? @Navaneel
Design of the experiment :

an earbud soaked in tap water is immersed into 5ml of Coconut water. The experiment is being carried out in a safe enclosed environment, adjacent to a doctor’s chamber.

Expectations : There should be no microbes present in the tap water submerged culture as it is chlorianted by the Municipality. Likewise, the surface culture on potato should not yeild any bacterial isolates.

Alternate Hypothesis : If there is presence of colonies of microbes on submerged or surface culture, there is contamination of microbes in the tap water being used for drinking resources.

Since most of us use this water in one way or the other without additional filtering, and there have been no untoward incident so far, I expect the microbes been enrichened are not pathogenic.
How about looking around for possible breeding places too? @Gaurangi Dhuri @Abhijeet Singh @Navaneel
Can Pagalapos be sites for mosquito breeding? @Abhijeet Singh @Gaurangi Dhuri Can’t we restart Pagalapos as model for many research studies? @Saida Sayyed @KIRAN CUBE @Shivam CUBE NEMATODE
Submerged culture : The bacterial growth happens in liquid media (Coconut Water)

Surface Culture : The bacteria growth happens on the surface of a solid medium (potato)
What is meant by resolution? @Abhijeet Singh @Navaneel @Gaurangi Dhuri @Aastha Ahuja Cube @Ishita Assam
[12:07 PM, 9/1/2021] +987289: Yess… we can predict the disease caused by mosquitoes especially dengue and chickenguniya as its caused by aedes mosquitoes… in which season or which particular month… and if we get collaboraters then we can find whether the location affects the causing of disease in particular seasons or months … @Abhijeet Singh right?
[12:08 PM, 9/1/2021] +917289: Yess… It can be great work… if we connect pagalapos and mosquitoes studies…
So what @Navaneel and I mean from ‘Submerged experiment’ is that
In one of the cases navaneel is using Coconut water as a source for growing microbes(if present) in tap water.

So @Navaneel used an ear bud and dipped in tap water and kept that in a container with coconut water. So that ear bud containing tap water sample is ‘submerged’ in coconut water.

I think I’m getting it correct @Navaneel . Please correct me if I’m wrong anywhere!
If I could please know what are pagalapos ?
You mean you want to predict Mosquito borne diseases in the area through this? Please clarify.
What is the incidence of Mosquito borne diseases in your locality, as per Department of Health?
[12:09 PM, 9/1/2021] +91 28389: yes
[12:09 PM, 9/1/2021] +91 28389: absolutely
[12:11 PM, 9/1/2021] Arunan HBSCE: Fresh coconut water? Because there will be possible differences in the microbe profile of fresh coconut water vs that was kept for sometime. Don’t you think so? What is your explanation on this? @Navaneel @Abhijeet Singh @Gaurangi Dhuri @Aditiya Joshi CUBE
[12:13 PM, 9/1/2021] Arunan HBSCE: Is the last sentence dealing with your Hypothesis? Can you state the Hypothesis more clearly as in Science, so that it can be verified or falsified? @Navaneel
[12:15 PM, 9/1/2021] +91 28389: Yes sir. Using fresh coconut water. But I want to do the exp with the remnant water as well, however I have not designed the exp yet.
[12:15 PM, 9/1/2021] +91 28389: yes sir
Please describe step by step procedure, how you prepare this set up of submerged culture? @Navaneel
Objective : Study of microbial contamination in Tap Water in Saltlake, Kolkata

Hypothesis : Due to proper Chlorination of water by the Municipality, I hypothesis that there are no microbes to be cultured from the Municipal water source.
Can’t we do this study of incidence of Mosquito borne diseases, across the country? @Gaurangi Dhuri @Abhijeet Singh @NavaneelStep 1 : Clean the coconut surface. Spray 70% alcohol to clean the cocnut outer shell. Ensure hands are washed to prevent contamination

Step 2 : Use a sterilised Rock / Sickle to break the coconut. Collect water with minimum contamination with cocnut fibres in Sterile collected

Step 3: use boiled muslin cloth (for sterilisation) to seperate fibres from the coconut water.

Step 4: (Variations from this step) → Take 5ml aliqout from cocnut water source.

Step 5 : Collect 10 ml tap water in sterilised collected and soak sterilised ear bud in tap water.

Step 6 : Dip Ear bud into 5ml coconut water aliquot in sterile collected.

Step 7: (Variations) → Close cap and seal with parafilm to prevent airborn contaminations.

Step 8 : Make 5ml cocnut water control in sample collecter. Exact steps as listed above are followed except dipping ear bud into the medium. The cap is closed and parafilmed.

Variations for parafilm : Dissolvable glue. Vaseline. Wax.

Variation in steps → Parafilm should not be used if the culture is aerobic. It may generate anaerobic conditions in brothHow will u proceed with experiment?
Is there any specific reason behind dipping the bud in tap water n then putting it in coconut water @Navaneel
[12:34 PM, 9/1/2021] +91 28389: Tap water is the inoculum.
[12:34 PM, 9/1/2021] +91 8389: Coconut water itself doesnt have any microbe in it for fermentation
[12:34 PM, 9/1/2021] +91 28389: it myst be exposed to air or inoculum
Okay… But how dipping the bud will be needed. Can we simply add a drop of tap water or 1ml of tap water as such directly in coconut water
i think the bud provides an additional substratum, in case the microbes are very les sin number. it is strictly not needed, but we used to do it in our lab when there was really less concentration of a culture. And also the bud provides a mesh to capture the microbes form the tap water, instead of the entire water entering into the media and diluting it, along with chlorine ions.
[2:24 PM, 9/1/2021] +91 88988 87289: Yess we can… we have to contact with BMC offices and collect the data of different wards and stated all over the country… and then we can compare it with our studies … and check whether our observations are going right or there is something which is missing… what do u think
@Abhijeet Singh @Hina Mudgal Kanpur CUBE @Arunan HBSCE ??
[2:28 PM, 9/1/2021] +91 88988 87289: Pagalapos are basically the holes that are present on the lid of the gutters… in the rainy season this holes will have water and other times it can be dry… these holes may have different organisms… there are even possibilities that pagalapos are the breeding site for mosquitoes @Navaneel …
Am I right @Abhijeet Singh @Arunan HBSCE @Hina Mudgal Kanpur CUBE … if I am wrong … pls correct me…
Please post some photos of Pagalapos sites from your locality @Gaurangi Dhuri @Abhijeet Singh

Breaking news. …pupae stop developing… I trapped flies on 8.30 pm on 29/07/2021 …after that I seen some larve on 1/08/2021 … it’s a open Bait setup in which I used tomato and cucumber as a bait…after that I seen some big larve on 4/08/2021 it’s a housefly larve …in that setup I seen two types of larve some are 3 mm in size and some are 5 mm in size I seen some pupue on 05/07/2021 some are in 3 mm size some are 5 mm in size after that I waiting for new flies some housefly emerge and one fruitfly emerged on 08/08/2021 but there so many pupae you can seen here they stopped developing I couldn’t seen from 15 days when I seen today I found to that pupae are not developing …. And why ? Location: Wardha data collector: janhvi deshmukh. Time :5.07 pm janhvi deshmukh homelab bajaj College of science wardha Maharashtra

Fruitfly and housefly pupae… Data collector: janhvi deshmukh location :wardha

Some fruitfly pupae…on tomato and some dead housefly … location:wardha. Data collector: janhvi deshmukh@Arunan HBSCE sir @Aswathy Suresh CUBE @Aditiya Joshi CUBE @KIRAN CUBE @Yash Sheregare CUBE @Batul Pipewala Kolkata mam and others…Biologists Just Got Closer to The DNA Secrets That Stop Species From Interbreeding : ScienceAlert

This is fascinating
Something that maybe cubists can think of testing in their fly cross breeding of strains from different parts of the country, to see if they aren’t compatible due to sattelite DNA

[KiranKalakotiR (Kiran Kalakoti)]

1st September 2021, 9.30pm to 2nd September 2021, 9.30pm
VIDEOS SHARED BY ASWATHY AT 11.27PM
Fruitfly Update

21 Days old culture bottle have ~10 Larvae, 8 Pupae, 1 Fly.

SLBK A22
Transfer Date : 12.08.2021
Transfer Flies : 1 Fly

Objective : To Find Drosophila Melanogaster from Native area.

01/09/2021
(observation today)

No of Flies : 1
No of Larvae : 10
No of Pupae : 8
No of Dead Flies : 1
Media Condition : Solidified

Update : 01/09/2021
Location : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
Fruitfly Update

21 Days old culture bottle have ~20 Larvae, 70 Pupae, 20 Flies.

SLBK B21
Transfer Date : 12.08.2021
Transfer Flies : 13 Flies

Objective : To Find Drosophila Melanogaster from Native area.

01/09/2021
(observation today)

No of Flies : ~20
No of Larvae : ~20
No of Pupae : ~70
No of Dead Flies : ~20
Media Condition : Solidified

Update : 01/09/2021
Location : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
Fruitfly Update

21 Days old culture bottle have ~20 Larvae, ~90 Pupae, ~60 Flies.

SLBK E17
Transfer Date : 12.08.2021
Transfer Flies : 14 Flies
Gravid : 6 ; Male : 8

Objective : To Find Drosophila Melanogaster from Native area.

01/09/2021
(observation today)

No of Flies : ~60
No of Larvae : ~20
No of Pupae : ~90
No of Dead Flies : ~30
Media Condition : Solidified

Update : 01/09/2021
Location : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
objective:- Prediction and prevention of mosquito born diseases Today(1st September 2021) I got 3 mosquitoes. None of them are Aedes! Observations noted in Log book and this is the photo of mosquitoes on a black background!
What is happening with E15, E16!? I think it’s important to follow them up also!
What do you think on this @Aswathy Suresh CUBE !?

VIDEOS SHARED BY SIDHY AT 12.00AM
SLTT-H
Single line culture of fruit flies.
Started on 16/08/2021, 11:40 AM
TRSV medium - 15/08/2021, 11:00 PM

Observation on 31/08/2021( 15 th day )
~ 14 larvae
2 pupae
~25 flies.

Location : Thanniam, Thrissur, Kerala
Sidhy.P.P, CUBE S N College , Nattika.
SLTT-I
Single line culture of fruit flies.
Started on 16/08/2021, 11:40 AM
TRSV medium - 15/08/2021, 11:00 PM

Observation on 31/08/2021(15 th day )
~18 larvae
7 pupae
~15 flies

Location : Thanniam, Thrissur, Kerala
Sidhy.P.P, CUBE S N College , Nattika.
SLTT-J

15 th day : No larva, No pupa , Less no. of flies , Fungal growth

Single line culture of fruit flies.
Started on 16/08/2021, 11:40 AM
TRSV medium - 15/08/2021,11:00 PM

Observation on 31/08/2021 (15 th day )
0 larva , 0 pupa , 4 flies
Fungal growth in the medium

Location : Thanniam, Thrissur, Kerala
Sidhy.P.P, CUBE S N College , Nattika.
Surely @Abhijeet Singh . SLBK E15 (58Days), E16 (43Days) are quite old previous subculture bottles of ‘E’ line. But its still have Flies, Larvae, Pupae…

SLBK E15 (58 Days) subculture bottle have ~7 Flies, ~5 Larvae, ~10 Unhatched Pupae.

SLBK E16 (43 Days) subculture bottle have ~15 Flies, ~10 Larvae, ~20 Unhatched Pupae.

(video will share tomorrow)
What is your further plan @Sidhy Kerala Drosophila?
summary

Fruitfly Culture
Location : Bottukadavu - Thrissur Kerala.

Objective : To Find Drosophila melanogaster from Native area.

Currently have 3 Single Line Culture ;

SLBK A, SLBK B, SLBK E

Maintain flies cultures by subculturing; Current subcultures are…

A Line - SLBK A22
B Line - SLBK B21
E Line - SLBK E15, SLBK E16, SLBK E17.

Description about each subculture bottles;
Current culture bottles which have flies, larvae, pupae

SLBK A22 (21 Days)
Transfer date : 12.08.2021
Transfer no flies : 1

(01/09/2021 - Observation)
No of Flies : 1
No of Larvae : 10
No of Pupae : 8
No of Dead Flies : 1
Media Conditions : Solidified

SLBK B21 (21 Days)
Transfer Date : 12.08.2021
Transfer no flies : 12
Gravid : 6 ; Male : 6

(01/09/2021 - Observation)
No of Flies : ~20
No of Larvae : ~20
No of Pupae : ~70
No of Dead Flies : ~20
Media Conditions : Solidified

SLBK E17 (21 Days)
Transfer Date : 12.08.2021
Transfer no flies : 14
Gravid : 6 ; Male : 8

(01/09/2021 - Observation)
No of Flies : ~60
No of Larvae : ~20
No of Pupae : ~90
No of Dead Flies : ~30
Media Conditions : Solidified

SLBK E16 (43 Days)
Transfer Date : 19.07.2021
Transfer no flies : 30
Gravid : 9 ; Male : 21

(01/09/2021 - Observation)
No of Flies : ~15
No of Larvae : ~10
No of Pupae : ~20
No of Dead Flies : ~40
Media Conditions : Solidified

SLBK E15 (58 Days)
Transfer Date : 02.07.2021
Transfer no flies : 15
Gravid : 8 ; Male : 7

(01/09/2021 - Observation)
No of Flies : ~7
No of Larvae : ~5
No of Pupae : ~10
No of Dead Flies : ~50
Media Conditions : Liquefied

Collaborators : @Arunan HBSCE sir @Yash Sheregare CUBE @Man Masih Beck RANCHI CUBE @Sidhy Kerala Drosophila @Rechel Turkey Cube Ranchi Moina @KIRAN CUBE ma’am @Lydia cUBe Drosphila ma’am @Aastha Ahuja Cube @Isha Pawle
Today 02/09/2021, there are only 2 flies in the bottle SLTT-J. Further plan is to transfer the flies to new TRSV medium.
[7:34 AM, 9/2/2021] Arunan HBSCE: So, you have a healthy SLBK A bottle culture with 10 Larvae and 8 Pupae. Are you sure? If so, that’s great news @Aswathy Suresh CUBE
Please write the summary of this. @Aswathy Suresh CUBE
[7:38 AM, 9/2/2021] Arunan HBSCE: Please post CUBE Bottukadavu HomeLab News in just three attractive lines.
To me, it looks like, @Aswathy Suresh CUBE has healthy A,B & E fruitfly Single Line cultures.
Please confirm @Aswathy Suresh CUBE
[9:25 AM, 9/2/2021] +91 8389: Breaking news from Navaneel’s home lab
[9:29 AM, 9/2/2021] +91 28389: Experiment 3 : bacterial culture of submerged culture specimen of tap water from saltlake kolkata

Continuation of images from 1/09/21.

In Exp 2, we had taken a ear bud full of tap water and inoculated coconut water medium. We had allowed incubation for 24 hours. Following the, we had streaked the sample on potatoes from expt 3 (as stated above). The potatoes are meant to act as a solid substrate for microbial growth. The potatoes were boiled, and the petri dishes were not sealed, but covered to allow aerobic respiration.

A control with no streaking was maintained as well

Pic A : Objective - to study bacterial contamination in tap water of saltlake kolkata. Date : 2/09/21 picture by : Navaneel Sarangi Location : Saltlake Kolkata.
In this picture we can observe a white mucilaginous coating growing on te surface of the potato. This picture is taken for reference before breaking sterility

contamination in tap water from saltlake, Kolkata. Date : 2/09/21 picture taken by : Navaneel Sarangi location : saltlake kolkata
In this picture we an seen clear mucilaginous covering and growth on the surface of the potato. I comparison this was the picture yesterday

We can trace vaguely the path of microbial growth along the potato. This also verifies that the bacterial growth was from the coconut water preferentially.

Pic c : This is an image of control. Objective : to study bacterial contamination of tap water in saltlake Kolkata date: 2/09/21 location : saltlake Kolkata picture by : navaneel Sarangi
We can observe no mucilginous growth. However there is certain red splotches. This I am not able to explain.

[9:43 AM, 9/2/2021] +91 98308 28389: Closer image of control
[9:45 AM, 9/2/2021] +91 98308 28389: The reason for growth can be : utility of a prior submerged culture step
2. Used of boiled potato
3. Use of aerobic conditions.

We will have to ascertain which ones of these are trueWhat is your immediate plan of work? @Sidhy Kerala Drosophila
[10:10 AM, 9/2/2021] Arunan HBSCE: Excellent! @Sidhy Kerala Drosophila
SLTT H fly culture is doing good on 15th day in TRSV medium.

Please write a summary of your SLTT cultures in not more than two sentences for capturing attention of readers and for potential collaborators. @Sidhy Kerala Drosophila
[10:17 AM, 9/2/2021] Arunan HBSCE: Can we assume that you have 3 different healthy SingleLine cultures from Bottukadavu locality?

If so, what is your immediate Plan of Work, to save them based on your earlier experience? @Aswathy Suresh CUBE

Don’t you think you have to have some urgent Immediate Plans? @Aswathy Suresh CUBE
[10:19 AM, 9/2/2021] Arunan HBSCE: @Sidhy Kerala Drosophila Please give your detail plan with date and time if preparing medium onward @Sidhy Kerala Drosophila
[10:20 AM, 9/2/2021] Arunan HBSCE: Please always give Location of the CUBE HomeLab and Date. @Navaneel

Very amused to see the potato is not sliced with a sharp knife with edges ‘eaten up’ @Navaneel Can’t we be more keen to *get a smooth slice like the smooth surface of the culture medium, in the petri dish? @Navaneel
yes sir I am trying that. With the boiled potato, it becomes highly unstable to get a perfect cut. Maybe a half boiled potato will be better stable to get a more smooth cut
Please give dates and after how many days etc., so that readers will find to follow you easily and join you as collaborators. @Navaneel
yes sir I mentioned here. I am sorry, I should have mentioned that the potatoes were streaked 24 hours earlier
Is this inoculated with coconut water?
What happened to your raw potato slices streaked day before yesterday?@Navaneel
[10:31 AM, 9/2/2021] Arunan HBSCE: When was this set up prepared? @Navaneel

What was your expectation and what is your observed result? Any comments? @Navaneel

And when was the photo taken, as date of observation? @Navaneel
[10:33 AM, 9/2/2021] Arunan HBSCE: Do we have any Controls? Don’t you think appropriate Controls are important for each experiment?
How will we redesign the experiment to convince all of our result? @Navaneel
What exactly is meant by ‘submerged’?
Please clarify @Navaneel
[10:38 AM, 9/2/2021] +91 98308 28389: As mentioned yesterday sir by @Abhijeet Singh and me, culture is a the growth of microbes in a favoured environment. There are various kinds of culture method, one of which is submerged culture. in this case the microbe is given a liquid medium of growth, so that the colony of microbe is submerged for growth inside the liquid.
[10:39 AM, 9/2/2021] +91 98308 28389: Sir I made 1 control, to compare between tap water exposed potato and no top water exposed potato. Ideally, we shoudl also have controls for boiled and raw potato, aerobic and anaerobic cultures as well.
[10:46 AM, 9/2/2021] Arunan HBSCE: How can we get potato slices reasonably similar to the petridish culture medium, instead of looking uneven and shattered?

How is boiled potato a simulation of petridish medium in conventional lab? This has been an important comparison while preparing alternate HomeLab medium for Fruitflies too.
So, let’s justify why slices of boiled potato can be simulation of lab medium for culturing microbes? @Navaneel @Abhijeet Singh @Aditiya Joshi CUBE and others
[10:48 AM, 9/2/2021] Arunan HBSCE: Please describe the method of preparing boiled potatoes from the beginning. @Navaneel for all to follow.

Please post short clippings of each stage of preparing the potato slice. @Navaneel
Hi @Navaneel, interesting experiment. I had a few questions about experiment design:

1. What is your control, can you explain?
2. How much sample have you added to your potatoes?

Can we do something to ensure that the growth is not from the coconut water itself?
[10:50 AM, 9/2/2021] Arunan HBSCE: Please photos of the whole sequence starting from freshly prepared potato slice till the latest observation with essential description and your conclusion, so far. @Navaneel
[10:55 AM, 9/2/2021] Arunan HBSCE: Are you having a liquid culture imitating nutrient broth culture?
Or you are using Potato to try simulating Nutrient Agar medium?
Please clarify before using complicated terms like submerged culture.
In case you have some reasons to use the term submerged culture please describe so that all can follow you and join you as collaborators. @Navaneel @Abhijeet Singh
[10:56 AM, 9/2/2021] Arunan HBSCE: Too much information in one sentence! Please clarify. @Navaneel
Yes sir, we are trying to develop a liquid culture to come as close as possible to Nutrient Broth. Potato is trying to simulate Nutrient Agar Medium sir.

For this, @Aditiya Joshi CUBE and I are trying to come up with a more standardised medium for bacterial growth using coconut water and some gelling agent like Rava.
Please finalize your solid medium simulation using boiled potato slices since you faced with several problems in your attempt.
Let’s resolve that first, since colony characteristics serves as important beginning and solid medium like boiled potato slices should help us, to start with.

What do you think needs to improved based on your initial attempt? @Navaneel
Let’s resolve this, first.
What is your comment? @Navaneel
@Aditiya Joshi CUBE please add on

Standardisation in potato medium :

1. How are we going to sterilise the medium ? Is boiling the entire potato enough ?
2. How can we create a plane surface with a soft boiled potato ?
3. Due to the inherent yellow colour of the potato, the colonies formed are often not visible. Is there any method to solve this issue ?
4. We usually overturn a petridish when colonies are growing to prevent condensed water varpour from falling on the medium. Since the potato is not fixed, the plate cannot be overturned.
   Please describe step by step, how did you do it last? @Navaneel
   And what improvement you would like to make this time, based on what you

VIDEO SHARED BY ISHA 11.31AM
2 Sep 2021
11:20 am
Worli, Mumbai
Fruitflies on lemon Slice
Process of making Potato-based Simulant of Solid Substrate (Prospective Nutrient Agar)

Step 1 : Wash Potatoes with soap and water to remove dust and contaminants. Soak in soap water to lyse most bacterial cells for 10 mins. (Not standardised : arbitary time taken - Need test for this)

Step 2 : Boild uncut potatoes (Standardisation required to extent of boiling. Essential for proper cut later on in experiment)

Step 3 : 70% ethanol wash of Potato for final sterilisation of cooled boiled potatoes

Step 4: Use sterile scalpel to careful make regular thin cuts with no potato peel in the medium.

Step 5 : Place potato in sterile petridish

Step 6 : Streak the surface of potato evenly.

Boiled Potato Slices replace to Nutrient Agar, at CUBE Sophia HomeLab, South Mumbai, Before transferring microbes from fermented coconut water, July 20, 2021 From CUBE Archives mcarunan
Modifications :
Need hard boiled potatoes. This will require modifications in the boiling steps.
Will need to pay closer attention to cutting the slices

Alternative :
Smash soft boiled potatoes and mash them till they are a hard bolus. Use this to fill into the petridish and make a flat surface wth a scalpel. Use dechlorinated or boiled and cooled water to adjust hardness of the potato medium.
Photos of each step with essential description, please @Navaneel
Also, i was wodnering about something. The most easily available pure cultures in the market which can be cultured comes from Yakult bottles. Since standardisation of the medium will require a stable replicating organism, and coconut water is still a natural uncontrolled process, we can use acified yakuly milk as a source of Lactobacillus casei Shirota strain.
Please restrict your description to what exactly, you had attempted the first time, with photos as evidence. @Navaneel
yes sir. I will send the photoes of the first time.
[11:44 AM, 9/2/2021] Arunan HBSCE: Let’s all understand through discussion.
Where exactly we need to improve. @Navaneel @Aditiya Joshi CUBE @Abhijeet Singh
[11:55 AM, 9/2/2021] Arunan HBSCE: With each photos please give essential description starting with objective in simple terms @Navaneel
Summary - SLBK A22
(subculture bottle of A line)

22 Days old Culture Bottle.

Date of Transfer Flies : 12/08/2021
Media Date : 11/08/2021
Transfer no of Flies : 1

Observation Based On Dates👇

12.08.2021 - Transferred 1 fly from A21 bottle to A22 bottle.

16.08.2021 - ~5 eggs, 0 larvae, 0 pupae, 1 fly

18.08.2021 - ~6 eggs, 0 larvae, 0 pupae, 1 fly

19.08.2021 - ~20 eggs, ~2 larvae, 0 pupae, 1 fly

21.08.2021 - ~20 eggs, ~3 larvae, 0 pupae, 1 fly

27.08.2021 - ~2 larvae, 2 pupae, 2 flies.

30.08.2021 - ~0 eggs, 1 larvae, 1 pupae, 1 fly, 1 dead fly.

01.09.2021 - ~1 fly, ~10 larvae (3mm) , ~8 pupae, 1 dead fly.
So what are your plans for these two cultures!?
Also are you able to see some microworms in these bottle culture? These are quite old cultures I think!
@Aswathy Suresh CUBE
What are we doing for saving A22!?
Do you think there is some problem with no.of flies in it!?
@Aswathy Suresh CUBE @Yash Sheregare CUBE @Man Masih Beck RANCHI CUBE
Let’s discuss on this!
What is your expectation in this bottle culture? @Aswathy Suresh CUBE And on what basis?

One Day After transferring microbes from Fermented Coconut water. Boiled Potato Slices replace to Nutrient Agar, at CUBE Sophia HomeLab, South Mumbai, July 21, 2021 From CUBE Archives mcarunan

Two Days After transferring microbes from Fermented Coconut water. Boiled Potato Slices replace to Nutrient Agar, at CUBE Sophia HomeLab, South Mumbai, July 22, 2021 From CUBE Archives mcarunan
Sir could you please say how you made the potatoes ?

Close up picture of colonies, two Days After transferring microbes from Fermented Coconut water. Boiled Potato Slices replace to Nutrient Agar, at CUBE Sophia HomeLab, South Mumbai, July 22, 2021 From CUBE Archives mcarunan

Control Three Days After Without transferring microbes from Fermented Coconut water. Boiled Potato Slices replace to Nutrient Agar, at CUBE Sophia HomeLab, South Mumbai, July 23, 2021 From CUBE Archives mcarunan

VIDEO SHARED BY ASWATHY AT 12.30PM
Yes @Abhijeet Singh observed some microworms are started to growing in SLBK E16 bottle (43 days old culture bottle) Video taken on 31.08.2021.
What is your comment on this picture? @Navaneel
Does this colony tell us anything based on colony characteristics and the source being fermented coconut water? @Navaneel Let’s look for some reference.
Let’s collaboratively move forward, by constructing knowledge, together.
Sir I was asking how you made the potatoes. Are they boiled, and how were the kept. Were the microbes from the broth which is growing there, or microbes fro air which later inoculated into the streaked coconut water
Are you finding the results interesting?

Or, does it look terrible?

Any interpretation from your side? @Navaneel
How about close up of other bottles, while searching for Nematode looking worms? @Aswathy Suresh CUBE
Why do you need so many steps will not boiling the potato be enough to kill all germs@@Navaneel
[1:36 PM, 9/2/2021] +91 28389: We will need to cool down the potatoes in air
[1:36 PM, 9/2/2021] +91 28389: If we don’t wash the potato properly, then the dirt will mix with the boiling water
[3:42 PM, 9/2/2021] Batul Pipewala Kolkata: @Navaneel you have a potential collaborator in Aashi who is in telegram CUBE grp she is a kalpana fellow interested in micro biology work.So please share your work in telegram Cube grp too
[3:46 PM, 9/2/2021] Batul Pipewala Kolkata: Join here Telegram: Join Group Chat
Also in short can you tell us what us the condition of cultures of @Yash Sheregare CUBE @Man Masih Beck RANCHI CUBE @Rechel Turkey Cube Ranchi Moina !?

Please post your homelab updates @Yash Sheregare CUBE @Man Masih Beck RANCHI CUBE!
Ok mam
Invitation to join today’s CUBE chatShaala,
Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in all CUBE what’s app groups and on metastudio after the session

Here’s the direct link of the CUBE webinar: https://webinar.hbcse.tifr.res.in/b/kir-hye-cn4

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.

Phyllanthus plants at Home Bhabha Center CUBE LAB are completely awake at 4.57pm today (2nd September 2021)

Accumulated water in an empty fallen coconut shell. Is this could be A possible breeding site of Aedes mosquitoes . Date of photo: 02nd September 2021. HBCSE CUBE LAB. @Abhijeet Singh @Gaurangi Dhuri @Saida Sayyed @Aswathy Suresh CUBE @Arunan HBSCE and others any comments. If this could be the possible breeding site then how do we prevent it?

Can we any sign of their End point of wakefulness and starting point of sleep Time: 5.20pm Date: 02nd September 2021. CUBE LAB HBCSE MANKHURD MUMBAI @Lakshmy PJ CUBE and othersYes definately this could be a possible breeding site for mosquitoes! If we see such accumulation of water we should remove the water and put the empty shell in dustbin!
Nice suggestion
Is there any way that we can find out which type mosquitoes can lay eggs here?
Yess … it can possibly be a aedes breeding site

BREAKING NEWS Revival of CUBE LAB HBCSE MANKHURD MUMBAI . 5 traps were kept at different places of HBCSE like 1. Dustbin area near HBCSE canteen 2. Dustbin area in Garden 3. CUBE LAB 4. HBCSE hostel. Tomato was used as a bait and traps were kept at 4pm today in the evening. Date of photo: 02nd September 2021 CUBE LAB HBCSEHow do we find it out?
By checking whether the mosquito larvae are present there… bz I think it would be difficult to find eggs in the shell…
@Aswathy Suresh CUBE @Lakshmy PJ CUBE @Gaurangi Dhuri @Abhijeet Singh @Saida Sayyed and others kindly join the webinar
After a long time! All the best! Is it Home Bhabha Center or Homi Bhabha Center for Science Education?
Yes Indeed
It’s CUBE LAB HBCSE TIFR

CUBE Ranchi ReMans’ Home Lab
02-09-2021
Fruitfly Update

Immediate Objective: to start a single line fruitfly culture(RNSL-B) line at ReMan’s Lab on TRSV Media

Short Term Objective: -To identify the trapped fruitflies
-To find native Drosophila melanogaster species from Ranchi

Media preparation- 18-08-2021
Transferred date- 19-08-2021

OBSERVATION
after 14 days of transfer
•Larvae ~ 5
•Pupae ~ 93
•Fly ~ 30

Highlights
•flies first appeared on 28th Aug(9th day after transfer)
•most larvae have turned into pupae
•all flies seen have red eyes
@Man Masih Beck RANCHI CUBE please join in Chatshaala. Interesting discussion on Fruitflies is going on. Please join.

[KiranKalakotiR (Kiran Kalakoti)]

2nd September 2021, 9.30pm to 3rd September 2021, 9.30pm

The Nobel Prize in Physiology or Medicine 2017

The 2017 Nobel Prize in Physiology or Medicine
jointly to
Jeffrey C. Hall, Michael Rosbash and Michael W. Young
for their discoveries of molecular mechanisms controlling the circadian rhythm

VIDEOS SHARED BY ASWATHY AT 1.34AM
Fruitfly Update

23 Days old culture bottle have ~3 Larvae, 14 Pupae, 1 Fly.

SLBK A22
Transfer Date : 12.08.2021
Transfer Flies : 1 Fly

Objective : To Find Drosophila Melanogaster from Native area.

03/09/2021
(observation today)

No of Flies : 1
No of Larvae : 3
No of Pupae : ~14
No of Dead Flies : 1
Media Condition : Solidified

Update : 03/09/2021
Location : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
Fruitfly Update

23 Days old culture bottle have ~20 Larvae, ~80 Pupae, ~25 Flies.

SLBK B21
Transfer Date : 12.08.2021
Transfer Flies : 12
Gravid Flies : 6 ; Male Flies : 6

Objective : To Find Drosophila Melanogaster from Native area.

03/09/2021
(observation today)

No of Flies : ~25
No of Larvae : ~20
No of Pupae : ~80
No of Dead Flies : ~20
Media Condition : Solidified

Update : 03/09/2021
Location : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
Fruitfly Update

23 Days old culture bottle have ~20 Larvae, ~120 Pupae, ~65 Flies.

SLBK E17
Transfer Date : 12.08.2021
Transfer Flies : 14
Gravid Flies : 6 ; Male Flies : 8

Objective : To Find Drosophila Melanogaster from Native area.

03/09/2021
(observation today)

No of Flies : ~65
No of Larvae : ~20
No of Pupae : ~120
No of Dead Flies : ~30
Media Condition : Solidified

Update : 03/09/2021
Location : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
Microworms present in SLBK A21 culture bottle. Date of observation 03.09.2021 : 12:45am. Further plan is to transfer this microworms from this culture to separate culture media bottle in order to identify them.
How can we transfer microworm from the media bottle @Aswathy Suresh CUBE
In yesterday’s Chatshaala we discussed the role of genes of circadian rhythm and their function discovered by scientists for which 2017Nobel prize was won.
The gene called period or per gene is controlled by negative feedback meaning when per gene switches on and forms per protein it will itself switch off the gene and prevent more per protein from being formed.During the day time per is degraded by the sunlight and hence cannot switch off its production and will continue to be made.
@Aswathy Suresh CUBE @Abhijeet Singh @Lakshmy PJ CUBE @Saida Sayyed @KIRAN CUBE and others

Objective:- prediction and prevention of mosquito born diseases. Yesterday i.e. 2/09/21 I got 6 mosquitoes. None of them are Aedes. Noted the observation in log book. Here is the photo of mosquitoes. Location:- Ulwe, Navi-Mumbai. Cube locality lab Ulwe.
@Gaurangi Dhuri please post your updates on mosquito studies!
@Aswathy Suresh CUBE @Batul Pipewala Kolkata @Seema @Shraddha Sonavane CUBE @Hina Mudgal Kanpur CUBE please post your updates like how are you planning for starting mosquitoes project in collaboration with us! If you have started please post your updates too!

Let’s have the data from different part of the country!
Equinox is approaching!
We can use brush - wet paint brush will help for to transfer worms in a new media.
Please add your comments on the photo @Sunita Chahar CUBE Ma’am!
Did you got more mosquitoes with time!?
What the situation of mosquitoes in your locality!?
Iam planing to take this flies for further experiments.

In SLBK E16, E14 subculture bottle I found some microworms started to growing. But I couldn’t able find out in E15 bottle.

Yes, E16 - 44 days old culture. E15 - 60 days old culture. E14 - 93 days old culture. These are quite old cultures.
Which species is this? Any guess? At what time of the day did you click this pic? Can you give details plz…
In A22 bottle currently have only 1 fly, ~14 pupae , ~3 larvae. So I am waiting for to get more progeny’s from this bottle. After to make more subculture at same time for to save A line.
Can you think of any reason of not finding worms in E15 bottle…
Expectations

transferred one fly, if it is proper gravid fly expecting 50 eggs per day.
So, 23rd Day expecting ~500 eggs, ~100 Larvae, ~300 Pupae, ~70 Flies.
[11:24 AM, 9/3/2021] Abhijeet Singh: So washing with water is not enough?
Soap water, ethnol and all are necessary?
I think if we just wash the potatoes with enough water so that the dirt goes will be enough.
As far as the germs and microbes in that dirt is concerned, I think they will eventually die when you are boiling the potatoes?
What is your interpretation on this @Navaneel @Aditiya Joshi CUBE
Also what is your interpretation on ‘Homelabs’ @Navaneel ?
If I don’t have ethnol then I won’t be able to do it? Or anyone!?
[11:28 AM, 9/3/2021] Abhijeet Singh: I think we need go through some references for that.

Aedes mosquitoes lays eggs in clean water and I think in this would be clean enough for them to lay eggs. Not Sure!

National Vector Borne Disease Control Programme (NVBDCP), Telangana, Dr. S. Prabhavathi said that while Aedes Aegypti genus mosquitoes breed in clean water, Anopheles and Culex genus mosquitoes breed in dirty water. However, the official said, Anopheles Stephensi which carries malaria-causing parasite in urban localities, breeds in waters stored in containers

Stagnant water breeds malaria, dengue

Because ma’am, this microworms are really small, I can only able to see when they curling in bottle side. Even if they are present in media,we can’t find out them.

E15 bottle is quite old culture and bottle wall is almost covered with empty pupal shells, dead flies etc . I checked them using proper light. But I couldn’t able to find out them in bottle wall area. May be they are present inside the media I guess.
How do we explain this?

So many pupae and no new flies? @Yash Sheregare CUBE @Aswathy Suresh CUBE @Lydia cUBe Drosphila @Saida Sayyed @KIRAN CUBE @Aastha Ahuja Cube
[11:29 AM, 9/3/2021] Dr. Sarita kumar Delhi CUBE: Yes…it’s a known fact…
[11:30 AM, 9/3/2021] Dr. Sarita kumar Delhi CUBE: E14 is older than E15…
@Navaneel please post your 2nd day observations with objective!
What did you expected and what did you get!?
Please Post your inference based on your expectations and observations in short with each setup!
Looking forward…!
Very interesting @Aswathy Suresh CUBE

When were you expecting fresh flies after observing so many pupae? @Aswathy Suresh CUBE
When did you start observing this bunch of pupae in SLBK A22? @Aswathy Suresh CUBE @Yash Sheregare CUBE
Are you happy? @Aswathy Suresh CUBE
Please describe, why.
Yes sir, I got one new progeny’s in between but That was died after some days .
Currently,~14 Pupae present inside culture bottle all are newly formed pupae. So I expecting next generation flies tomorrow & day after tomorrow.
Are you happy with this culture SLBK B and why?@Aswathy Suresh CUBE

What is your immediate plan of work? @Aswathy Suresh CUBE
Amazing capture! @Aswathy Suresh CUBE did you capture this through phone microscopy or normal phone camera adjustments!?
E15 - 60 days still have flies, Larvae, Pupae. E14 - 93 days old culture have no flies, no larvae, no pupae.

E14 bottle is much easy to check microworms because that bottle quite look empty.
Interesting! @Batul Pipewala Kolkata What exactly is negative feedback in gene expression and what is its implication?

Let’s explain this separately as a topic for all Cubists to grapple. @Batul Pipewala Kolkata

Another point is
surely, there must be some references you are banking on. Please post with an introduction how to read that reference article and which part is relevant in that reference article.@⁨Batu@Batul Pipewala Kolkata

Observed a Mimosa plant with beautiful flowers in my locality Mahad, Raigad; today morning at 7:30 a.m. Started the day with seeing its response to touch!
Expecting next generation flies on 04/09/2021 or 05/09/2021.
PREDICTION AND PREVENTION OF MOSQUITO BORNE DISEASES
A great idea to pursue in All India level and in Sri Lanka @Abhijeet Singh
What is your idea to rope in cubists from across the country before Autumnal Equinox, September 22,23
[11:48 AM, 9/3/2021] Arunan HBSCE: How do we improve the photo quality to make sure the features we are looking for is clear? @Abhijeet Singh @Gaurangi Dhuri @KIRAN CUBE @Raj Yashwantrao Patil CUBE @Ishita Assam @Aastha Ahuja Cube
[11:49 AM, 9/3/2021] Arunan HBSCE: What is special about Equinox? @Abhijeet Singh And what is it?
Recently, around 01.09.2021 observed ~8 pupae in A22 bottle.

Before that I got 2 pupae 24.08.2021 & 1 new fly on 27.08.2021.
Please post appropriate videos for all to review and not miss it.

We had earlier experience that SOMETHING WE MAY MISS, BUT OTHERS POINT THIS OUT TO US
@Aswathy Suresh CUBE
Yes, Now I don’t have much worry about A line culture. Because, at lest got some larvae & pupae inside A22 subculture bottle. So, I am expecting some new flies will emerges today or day after tomorrow as well.
[11:55 AM, 9/3/2021] Arunan HBSCE: When did you first notice the pupae so that you can expect when exactly, flies can emerge @Aswathy Suresh CUBE
[11:57 AM, 9/3/2021] Arunan HBSCE: Please post close up pictures of these precious SLBKA Pupae @Aswathy Suresh CUBE
Please describe how they look, according to you and when you expect them to give rise FRESH FLIES.
We must have some Expectation based on the day you started seeing LARVAE TURNING TO PUPAE😇 @Aswathy Suresh CUBE
Taken this video using normal phone camera, first focused area, then adjusted and zoomed at particular part & taken video.
So, basically you are not able to locate flies in E15, though it may have…right?
Actually ma’am , E15 culture still going culture… Mess up with lots of pupae (Hatched & Unhatched Pupae Shell) & dead flies. This case I couldn’t able to locate microworms inside culture.
[12:38 PM, 9/3/2021] Batul Pipewala Kolkata: Yes please post your observations on interesting work you are doing @Navaneel
[12:41 PM, 9/3/2021] Batul Pipewala Kolkata: Reference:https://www.nobelprize.org/prizes/medicine/2017/press-release/
@Abhijeet Singh can you take a close up photo of one mosquito each and place them to be able to see their back so markings on thorax can be seen

VIDEOS SHARED BY YASH AT 1.12PM
Updates on Fruit fly cultures
Location - Prabhadevi, Mumbai
Breaking News
SLPbA3
Highlight - On 7th day from transferring 3 fruit flies, there are no larvae seen today.
Alert - There are some black fungi like structures Growing in this media
I am suspecting that While transferring fruit flies from SLPbA2 culture, there could be possibility of transfer of unwanted fungi that was seen near the cotton plug

Media date* - 26/08/2021
Transfer date - 27/08/2021
No of flies transferred on 27/08/2021 - 3
Observation - No Larvae or pupae seen still on 7th day

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality
Immediate plan to save SLPbA3
This is what I think can be done

1. Transferring the 3 fruit flies to a new medium TRS-V media bottle - (I have additional one TRS-V media bottle that was made on 26/08/2021)
2. Observing if the fruit flies lay eggs in the new media as they haven’t yet in SLPbA3
   culture
   Any other suggestions from CUBists? @Aswathy Suresh CUBE @Man Masih Beck RANCHI CUBE @Isha Pawle @Abhijeet Singh @Arunan HBSCE sir @Sidhy Kerala Drosophila and others
   Please post close up photos for others to check @Aswathy Suresh CUBE
   Have we standardised the TRSV medium preparation?
   Have you followed that? @Yash Sheregare CUBE In that case, how do we explain for the presence of fungi? @Yash Sheregare CUBE @Aswathy Suresh CUBE @Sidhy Kerala Drosophila @Aastha Ahuja Cube @Man Masih Beck RANCHI CUBE @Rechel Turkey Cube Ranchi Moina
   Updates on Fruit fly cultures
   Location - Prabhadevi, Mumbai
   SLPbB3 - 7th day
   Highlights - SLPbB3 shows 40 pupae and a ~ 20 3rd instar larvae on 7th day of transfer of fruit flies

Media date* - 26/08/2021
Transfer date - 27/08/2021
No of flies transferred on 27/08/2021 - 30

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality

3/09/2021
No of flies - 28
No of larvae - 20- 3rd instar larvae, - 10- 2mm larvae near media
No of pupae - 40
No of dead flies - 2
What is the summary of all cultures at CUBE Prabhadevi with BREAKING NEWS? @Yash Sheregare CUBE
Are you happy? @Yash Sheregare CUBE
We cannot understand what exactly is the sum of the whole story of Single Line Cultures at CUBE HomeLab Prabhadevi @Yash Sheregare CUBE

Surely, you can help by starting with Breaking News @Yash Sheregare CUBE
[1:37 PM, 9/3/2021] Yash Sheregare CUBE: Updates on Fruit fly cultures
Location - Prabhadevi, Mumbai
SLPbC3 - 7th day
Breaking News Highlights Only 5 pupae and 5- 3rd instar larvae seen on 7th day of sub culture on 27/08/2021

Media date - 26/08/2021
Transfer date - 27/08/2021
No of flies transferred on 27/08/2021 - 35

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality

3/09/2021
No of flies - 33
No of larvae - 5 mm larvae
No of pupae* - 5
No of dead flies - 3

Expectations
31/08/2021 - Pupae and larvae - 10-20
*1/09/2021 - More pupae - 20+
3/09/2021 - More pupae - 30
4/09/2021 - Newly hatched fruit flies
[1:43 PM, 9/3/2021] Yash Sheregare CUBE: Yes I have followed the exact protocol followed by Other collaborators for making TRS-V Medium

The presence of Fungi can be explained as 1) in previous culture that is SLPbA2 ( - there was this black fungi seen in the culture and also near cotton plug
2) So while fruit flies were transferred, I am suspecting that the fungi was also transmitted along with fruit flies
3) Another Goof up I think is that the SLPbA2 (previous culture) had lose cotton plug so probably that can also be a reason for fungi like contaminants to enter inside the Media.
@Arunan HBSCE sir @Isha Pawle @Aswathy Suresh CUBE @Man Masih Beck RANCHI CUBE @Sidhy Kerala Drosophila
Does that mean that the antagonistic property of vinegar is for a period of time and not throughout the maintenance of an culture bottle?
Since it seems @Yash Sheregare CUBE suspects that fungi were stuck to cotton plug and may have developed later when anti fungal agents lost their effects.
@Aswathy Suresh CUBE @Yash Sheregare CUBE

[KiranKalakotiR (Kiran Kalakoti)]

3rd September 2021, 9.30pm to 4th September 2021, 9.30pm

Date:25/8/2021
Time:6:57pm
Place: Mampilly
Floral bud started developing+ 4th compound leaf arising same time.

Breaking news…pupae stop developing… trapped flies on 8.30pm on 29/07/2021… after that I seen some larveon 1/08/2021 it’s a open bait setup in which I used tomato and cucumber as a bait… after that I seen some big larve on 4/08/2021 it’s a housefly larve… in that setup I seen two types of larve some are 3 mm in size and some are 5 mm in size I seen some pupue le on 5/08/2021 some are 3 mm in size and some are mm in size after that I waiting for new flies some housefly emerge and one fruitfly emerged on 08/08/2021 but there are so many pupae you can seen here they stopped developing I couldn’t seen from 15 days when I seen today I found to that are not developing… … and why? …
Location: wardha
Data collector: janhvi deshmukh
Time:2.31 pm
Janhvi deshmukh homelab bajaj collage of science Wardha
@Arunan HBSCE sir @Aswathy Suresh CUBE @KIRAN CUBE @Batul Pipewala Kolkata mam @Yash Sheregare CUBE and others

Some fruitfly pupae and some housefly pupae…@Arunan HBSCE sir @Yash Sheregare CUBE @Aswathy Suresh CUBE @Batul Pipewala Kolkata mam and others

Some dead housefly and some fruitfly pupae…@Arunan HBSCE sir @Yash Sheregare CUBE @Aswathy Suresh CUBE @KIRAN CUBE mam @Batul Pipewala Kolkata mamGreat @Lakshmy PJ CUBE
How many days does this plant take to develop first Floral bud

Long term objective -Floral dip method
Short term objective -

1. To observe life cycle
2. observing heteroblasty(To observe the transformation from simple to compound leaves.
   3)Identifying cardamine plants

Date:27/8/2021
Time:1:54pm
Place: Mampilly
Flower of cardamine + 4th compound leaf.

Same point in which cardamine flower and compound leaf arising.

Date:28/8/2021
Time:6:30pm
Place: Mampilly
Fruit started developing

Date:29/8/2021
Time:6:12pm
Place: Mampilly
The fruit is developing + New floral buds developing

Date:31/8/2021
Time:6:02pm
Place: Mampilly
The fruit is developing.

Date:31/8/2021
Time:6:02pm
Place: Mampilly
Colour change noted on leaves.

Date:1/8/2021
Time:6:28pm
Place: Mampilly
The fruits is developing.Colour change noted on leaves.

Date:1/8/2021
Time:6:28pm
Place: Mampilly
The fruits is developing.Colour change noted on leaves.

Date:3/8/2021
Time:3:06 pm
Place: Mampilly
3 fruit on one single plant. The fruit development in a dichotomous manner.

Cardamine plants in coconut shell.Date:3/8/2021
Time:3:06 pm
Place: Mampilly

Date:3/8/2021
Time:3:15 pm
Place: Mampilly
Flower bud and 4th compound leaf developing simultaneously.
27days later on germination.@KIRAN CUBE The seeds germinated on 30/7/2021.On 25/8/2021 first floral bud.
In how many Cardamine plants you have observed the floral bud after 27 days of seed germination?
[3:40 PM, 9/3/2021] Aswathy Suresh CUBE: Pupae first note in A22 bottle on 27.08.2021. and after 01.09.2021 observed ~8 Pupae.
[4:34 PM, 9/3/2021] Aswathy Suresh CUBE: What about other culture bottles @Yash Sheregare CUBE?
What is your further plan @Man Masih Beck RANCHI CUBE?
How many days old bait is this @Janhvi Deshmukh ? Did you tried with new bait, in order to trap fruit flies?
One month @Aswathy Suresh CUBE

VIDEOS SHARED BY VISHNUPRIYA AT 5.31PM
Update on ant model system .

Long term Objective: how does ants communicate?

Started ant model system on April 21st 2021 by feeding ants with honey and Later on
On 22nd April created ant formicarium for closer observation of ants .

But unfortunately due to health and other issues I couldn’t continue with it . And yesterday on 2nd of September I saw some ants under wet wooden logs . And they were orange yellow ish in colour .

Placed a jar filled with soil and sugar on top and today on 3rd September 2021 they
Completely covered the whole jar .

How do we identify what type of ant it is ? @ Seethalakshmi

Date : 3rd September 2021
Time : 5.29 pm
Location: alapad thrissur kerala
Collected by: vishnupriya cj cube sn nattika
[5:31 PM, 9/3/2021] Vishnupriya CUBE: Update on ant model system .

Long term Objective: how does ants communicate?

Started ant model system on April 21st 2021 by feeding ants with honey and Later on
On 22nd April created ant formicarium for closer observation of ants .

But unfortunately due to health and other issues I couldn’t continue with it . And yesterday on 2nd of September I saw some ants under wet wooden logs . And they were orange yellow ish in colour .

Placed a jar filled with soil and sugar on top and today on 3rd September 2021 they
Completely covered the whole jar .

How do we identify what type of ant it is ? @917994274817

Date : 3rd September 2021
Time : 5.29 pm
Location: alapad thrissur kerala
Collected by: vishnupriya cj cube sn nattika
[5:40 PM, 9/3/2021] Vishnupriya CUBE: Update on butterfly model system.

Till now ( 3rd September 2021) observed 26 species of butterflies in my locality.

Out of this list , life cycle of 7 butterflies were studied.
•common crow
• common mime
• peacock royal
•common mormon
• common emigrant
• commander
• pysche butterfly

Small term objective : study life cycle of butterflies
And butterfly diversity

Long term Objective:
What exactly is mimicry? study more about butterflies that show mimicry .

Date : 3rd September 2021
Time : 5.43 pm
Location: alapad thrissur kerala
Collected by : vishnupriya cj cube sn nattika
Update on butterfly model system

Keerthana balakrishnan of SN college nattika currently pursuing her degree in malayalam language was so much interested to know more about butterflies and started to observe a lot more than before .

And its so good to see people from other fields showing interest in these things .
Today her 1st pupa hatched and its a papilio polymnestor ( blue mormon) . Video created by keerthana balakrishnan
Date : 3rd September 2021
Time : 5.50 pm
Collected by: keerthana balakrishnan sn college nattika

Single line culture bottles of fruit flies. TRSV medium prepared on 15/08/2021. Transferred gravid fly on 16/08/2021.
There is fungal growth only in SLTT - J bottle. Lost that culture. Fungal growth was observed from 14 th day. In the same bottle , the number of pupae and new generation flies emerged is less. Only five new generation flies formed. One fly got stuck in the medium on the same day it emerged. Other two young flies and mother fly died on 16 th day (There had fungal growth in the medium). Two flies were remaining on 02/09/2021.Not gravid flies. Before transferring them to medium bottle , I tried to transfer them to an empty bottle to remove(complete removal not possible) the fungal particles stick to their body. Because , the flies were walking through the fungi. While transferring , one fly escaped. Remaining one was a small fly. I think it was male. Even though the new medium was ready , I didn’t transfer the fly into the medium. That fly also died. So, lost the SLTT-J culture.

03/09/2021
Thanniam, Thrissur, Kerala
Sidhy.P.P , CUBE S N College, Nattika.

TRSV medium prepared on 02/09/2021 to subculture SLTT-H , SLTT-I and SLTT-J. But there are no J flies to transfer. Lost that culture.
03/09/2021
Location: Thanniam , Thrissur , Kerala.
Sidhy.P.P , CUBE S N College, Nattika.

I found something interesting in my courtyard. Today on 3rd of September 2021.

That place is completely filled with weeds ( green colour) maybe a weed called Japanese stiltgrass.( not sure. If wrong pls do correct me)
But I came to notice one among them was different. It had a white and green colour combination on its leaf and was so beautiful and unique from others .

Why did that particular one have a colour variation . It has got 3 branches and all the 3 coming from the root are having green and white colour combination

Why there’s a change on its colour ? .

Time: 7.25pm
Date : 3rd September 2021
Location: alapad thrissur kerala
Collected by: vishnupriya cj cube sn nattikaI found something interesting in my courtyard. Today on 3rd of September 2021.

That place is completely filled with weeds ( green colour) maybe a weed called Japanese stiltgrass.( not sure. If wrong pls do correct me)
But I came to notice one among them was different. It had a white and green colour combination on its leaf and was so beautiful and unique from others .

Why did that particular one have a colour variation . It has got 3 branches and all the 3 coming from the root are having green and white colour combination

Why there’s a change on its colour ? .

Time: 7.26pm
Date : 3rd September 2021
Location: alapad thrissur kerala
Collected by: vishnupriya cj cube sn nattika
It looks like the green colour chlorophyll is expressed in some parts and not expressed in other parts giving it a pattern of green and white.

[KiranKalakotiR (Kiran Kalakoti)]

4th September 2021, 9.30pm to 5th September 2021, 9.30pm

Close up pictures of A22 subculture bottle. ~10 more pupae + ~2 larvae observed today. Expecting new flies will emerg today/tomorrow. Picture taken 03/09/2021 : 11:09pm.

VIDEOS SHARED BY ASWATHY AT 11.40PM TO 1.44AM
[11:40 PM, 9/3/2021] Aswathy Suresh CUBE: Closeup video of E15 subculture bottle @Dr. Sarita kumar Delhi CUBE ma’am @Arunan HBSCE sir… We can able to see lot of dead flies body’s accumulated in bottle wall side and pupae shell also can able to see inside the culture bottle. Can’t able to see any microworms in bottle wall area.
[11:52 PM, 9/3/2021] Aswathy Suresh CUBE: Close up pictures of A22 subculture bottle. ~10 more pupae + ~2 larvae observed today. Expecting new flies will emerg today/tomorrow. Picture taken 03/09/2021 : 11:09pm.
Fruitfly Update

24 Days old culture bottle have ~2 Larvae, ~10 Pupae, ~4 Newly Emerged Flies .

SLBK A22
Transfer Date : 12.08.2021
Transfer Flies : 1

Objective : To Find Drosophila Melanogaster from Native area.

04/09/2021
(observation today)

No of Flies : 4 Flies (Including mother fly)
No of Larvae : 2
No of Pupae : 10
No of Dead Flies : 1
Media Condition : Solidified media

Update : 04/09/2021
Location : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
Fruitfly Update

24 Days old culture bottle have ~10 Larvae, ~90 Pupae, ~27 Flies .

SLBK B21
Transfer Date : 12.08.2021
Transfer Flies : 12
Gravid flies : 6 ; Female flies : 6

Objective : To Find Drosophila Melanogaster from Native area.

04/09/2021
(observation today)

No of Flies : ~27
No of Larvae : ~10 (3mm)
No of Pupae : ~90
No of Dead Flies : 30
Media Condition : Solidified media.

Update : 04/09/2021
Location : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
Fruitfly Update

24 Days old culture bottle have ~15 Larvae, ~125 Pupae, ~55 Flies .

SLBK E17
Transfer Date : 12.08.2021
Transfer Flies : 14
Gravid flies : 6 ; Female flies : 8

Objective : To Find Drosophila Melanogaster from Native area.

04/09/2021
(observation today)

No of Flies : ~55
No of Larvae : ~15
No of Pupae : ~125
No of Dead Flies : ~40
Media Condition : Solidified media.

Update : 04/09/2021
Location : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
SLBK E15 - 62 Days old culture bottle (Transfer date of bottle 02.07.2021) still have ~10 flies, ~10 Unhatched pupae, ~2 Larvae .Video Date : 04/09/2021 ; 12:15am. Home Lab : Bottukadavu - Thrissur Kerala.
So actually, Aedes Mosquitoes have White stripes on their legs so we can differentiate between non-aedes and aedes.

What we do is:
After we catch the mosquitoes we keep them on a black background. If the Aedes mosquito is there then in the black background the white stripes are clearly visible.

That’s how we identify Aedes.
Ohh thanks for the information! I will also try to identify Aedes mosquitoes nearby.
There are two types of Aedes …Aedes aegypti and Aedes albopictus so it’s look different from each other @Abhijeet Singh
What is the difference?
I don’t know that’s why I am asking @Manadi Cube
[1:53 PM, 9/4/2021] Arunan HBSCE: Wah, wah! @Manadi Cube
[1:54 PM, 9/4/2021] Arunan HBSCE: Great @Abhijeet Singh Any reference? @Abhijeet Singh @Manadi Cube @Gaurangi Dhuri
[1:55 PM, 9/4/2021] Abhijeet Singh: Actually we have gone through the Aedes species. We just see the stripes if present.
[1:56 PM, 9/4/2021] Abhijeet Singh: We have to check more on that
Firstly, how do we collect mosquitoes from our surroundings?
Can we also look around for Mosquito breeding sites? @Manadi Cube
Because Aedes aegypti and Aedes albopictus they both have white stripes …@Abhijeet Singh
Why not look for references and post the references here? @Janhvi Deshmukh @Abhijeet Singh @Manadi Cube @Hina Mudgal Kanpur CUBE and others
Yes sir @Arunan HBSCE sir

The circled once are Aedes. On a black background the stripes are visible. This pic is from 2017 National meet Presentation.
So another are anopheles? @Abhijeet Singh
[2:01 PM, 9/4/2021] Abhijeet Singh: Not sure😅
[2:02 PM, 9/4/2021] Abhijeet Singh: I posted the pic as a reference to how we can differentiate Aedes and Non-Aedes.
Online Healthcare & Medical Courses - FutureLearn difference between Aedes aegypti and Aedes albopictus but the look so similar
Ok @Abhijeet Singh
[3:17 PM, 9/4/2021] Manadi Cube: According to my knowledge, mosquitoes breed in stagnant water. So we must look for mosquitoes in stagnant water.
[3:18 PM, 9/4/2021] Manadi Cube: Around stagnant water*
Legs as well as abdomen

@Janhvi Deshmukh …See this picture. One is of Aedes albopictus nd one is of Aedes aegypti. Observe the difference and try to find out, which one is which…
Which one is Aedes aegypti mam @Dr. Sarita kumar Delhi CUBE mam ?
[3:49 PM, 9/4/2021] Dr. Sarita kumar Delhi CUBE: Left one…look for references now.
[3:51 PM, 9/4/2021] Dr. Sarita kumar Delhi CUBE: https://www.oecd-ilibrary.org/sites/9789264302235-5-en/index.html?itemId=/content/component/9789264302235-5-en

Read through👆
Also, how do you look for Aedes mosquito without being bitten by it? @Abhijeet Singh

[4:48 PM, 9/4/2021] Sunita Chahar CUBE: Phyllanthus grown by seeds
[4:49 PM, 9/4/2021] Sunita Chahar CUBE: 4 to 5 leaves in 14 days
[4:50 PM, 9/4/2021] Sunita Chahar CUBE: After germination
[4:50 PM, 9/4/2021] Sunita Chahar CUBE: Seeds took almost 8 to 10 days to germinate
The one fly that I transferred on 12.08.2021 from SLBK A21 to A22 bottle, that time not much sure it’s gravid or not.

6 days later, after transferred that one fly (18.08.2021) - Eggs were started present inside te culture bottle.

(7 days after) Next day 19.08.2021 - Observed 2 (3rd instar) larvae inside bottle wall. Transferred fly still alive.

15 days after (27.08.2021) - there is 2 flies (including transferred fly).

21 days later (01.09.2021) observed ~8 Pupae ~3 larvae 1 alive fly. & 1 dead fly.

(This data based on observation)

Goof ups : observation in between missed (e.g : first fly emerged date, first pupae, first 1st instar larvae etc).[5:04 PM, 9/4/2021] Aswathy Suresh CUBE: I think yes, because that transferred one fly also laying Eggs (~20) and give some Larvae (~3) , Pupae (~2) inside culture. But the issue is that it’s taken more days to hatch to from larvae then larvae to pupae…
[5:37 PM, 9/4/2021] Aswathy Suresh CUBE: Yes sir, around these days I am very happy about SLBK B culture because currently its have good number of Larvae (~20), Pupae (~80), Adult flies (~20).
"Genetics of Aquatic Organisms2021, Vol 5, Num, 2 (Pages: 77-85) Abstract Full Text:PDF Recommended articles Mail to Author How to Cite
A Cost-effective DNA Isolation Strategy from Crustaceans Enables the First Molecular Phylogenetic Identification of Moina macrocopa from India
Shital Bhanushali 1-2 ,Kritika Katti 2 ,Jyoti Ramchandani 2 ,Subhojit Sen 1
1 Mumbai University, School of Biological Sciences, UM-DAE Centre for Excellence in Basic Sciences, Kalina Campus, Santacruz (E), Mumbai 400098
2 University of Mumbai, Department of Life Sciences, Vidyanagari, Santacruz (E), Mumbai 400098
DOI : 10.4194/2459-1831-v5_2_04
Viewed : 417 - Downloaded : 154
We report the first barcode identification of water flea species Moina macrocopa (strain JSK1) from th…
NATIONAL CUBE MEET-2021
(October 1st-3rd 2021)

Hello everyone,

We’re so excited to share the news that we’re organizing NATIONAL CUBE MEET -2021 and this time the month of the National meet is also coinciding with the announcement of Nobel Prize 2021.

The event will be of three days from October 1st -3rd 2021 in the evening from 5.30pm to 9.30pm and the program will also be broadcast live on YouTube

Save the dates
1st Oct 2021-Friday
2nd Oct 2021-Saturday
3rd Oct2021- Sunday
Timing: 5.30pm to 9.30pm

EVENT DETAILS WILL BE SHARED SOON

Close -Up view of culture ant. Iam observed 3 hairs in the head part. And its legs and antenna is long. This is also an identical feature. Body colour is brownish black. Some times it shows blue in colour.
Location: pazhuvil
Date: 1/9/21
Time:3:29 pm
Data collector: Seethalakshmi

[KiranKalakotiR (Kiran Kalakoti)]

5th September 2021, 9.30pm to 6th September 2021, 9.30pm

It’s a white moth? Looks beautiful … Data collector: janhvi deshmukh … time:10.05 pm. Date :04/09/2021

@Aswathy Suresh CUBE in the culture bottle SLBK A22 there are new progenies including ~ 2 larvae , ~10 pupae , ~4 new flies. So, are they progenies of the that mother fly which was transferred to make single line?
Actually @Rechel Turkey Cube Ranchi Moina,
SLBK A21 subculture bottle I transferred ~5 flies from A20 bottle after few days later only one fly were left in SLBK A21 subculture bottle, other flies were died and didn’t get any next generation progeny’s from that bottle.That one fly I transferred in to A22 bottle (current subculture bottle).

Rechel’s CUBE Homelab morabadi Ranchi

Date: 04/09/2021

Fruit fly report

Currently having 3 native fruit fly culture bottles : RF-IIb , RF-IIC, RF-IIC’.

RF-IIb :transfer date(TD) 19/06/2021
Media Date (MD) 06/06/2021 ~ 30 flies present. Pupae and larvae are present.

RF-IIC and RF-IIC’ :
TD and MD unknown
( Goofup : didn’t mentioned the date while transferring. But it was transferred in mid-july)

~ 50 fruit flies are present in both ( RF-IIC and RF-IIC’) culture bottles. Pupae and larvae are also present.

TOMORROW’S PLAN

To make TRSV medium culture bottles to make single line
(for Reman’s lab as well)

7 Bottles were washed on 16/08/2021.
Requirements for TRSV medium.
1 Tomato : medium sized
Rava : 2 table spoon (~30g)
Sugar : 1 table spoon (~15g)
Vinegar: 1 table spoon ( ~15ml)
Water : ~ 150ml

Collaborators: @Aswathy Suresh CUBE @Man Masih Beck RANCHI CUBE @Yash Sheregare CUBE@Sidhy Kerala Drosophila and others .

VIDEOS SHARED BY RECHEL AT 12.30AM
[1:19 AM, 9/5/2021] Aswathy Suresh CUBE: Immediate plan is to make new TRSV media and transfer these flies from this 25 days culture bottle to new fresh media bottle in order to maintain this cultures.
[1:25 AM, 9/5/2021] Aswathy Suresh CUBE: Yes sir, I am waiting for to get more number flies from A22, B21 bottles. After getting enough number then Iam planing to transfer these flies in to new media bottle.
[1:30 AM, 9/5/2021] Aswathy Suresh CUBE: On 30.08.2021 dead fly is quite small, looking their abdomen it seemed Male fly.
[1:38 AM, 9/5/2021] Aswathy Suresh CUBE: Different stage larvae that I observed on 01.09.2021. Larvae started appear on bottle then only I started to noticed them…! Some earlier pupation date of formation also I missed out.

Yes, it is a kind of goof up that didn’t noted larvae earlier inside the culture. Not taken continues date data.
[1:45 AM, 9/5/2021] Aswathy Suresh CUBE: I think these all progeny’s came from this one fly that I transferred on 12.08.2021. (Even it may not be fully gravid fly).
Because I observed some eggs in bottle wall after transferred this fly. but it taken more time to get hatch to form larvae. Then larvae to pupae I guess.

VIDEO SHARED BY ARUNIMA AT 2.22PM
Date:5/09/21. Cardamine plant growing in open soil.a close relative of Arabidopsis thaliana.Belongs to the family Brassicaceae (mustard family)

New Phyllanthus experiment to study
1.Phenology pattern of plant
2.sleep wake cycle
3.studying the Effect of light on opening and closing of leaves once again

[5:46 PM, 9/5/2021] Sunita Chahar CUBE: Researchers recycle shrimp waste into supplements
[5:47 PM, 9/5/2021] Sunita Chahar CUBE: Can we recycle prawns without chemical treatments

I put the seeds on the soil, on August 16. and they are started germinating on 22 agu .and on 23 there are 3 leaves and on 26th Aug there are 4 leaves.
Which seeds did you sow?
Phyllanthus maam.

There are very few leaves in this plant and they started fruiting.
At how many leaved stage, the plant flowered
As an aside: https://kids.frontiersin.org/
Maam 16,17 leaves are there

CUBE Ranchi ReMans’ Home Lab
05-09-2021
Fruitfly Update

Alert Alert Alert
Immediate Objective: to save the RNSL-B culture by transferring the flies into new TRSV Media bottle

New Media preparation- 05-09-2021, 6 am

Planned Day to transfer flies
06-09-2021; 6 am

OBSERVATION
-On 4th September morning, it was observed that the TRSV Media was turning liquid and thus many flies were dying.
-currently around 8 flies are seen but none seems to have a bulging body.

Highlights
•flies first appeared on 28th Aug(9th day after transfer)
•all flies seen have red eyes
•4th Sep- media turns liquid
•5th Sep- new media bottles prepared
@Rechel Turkey Cube Ranchi Moina @Aswathy Suresh CUBE @Yash Sheregare CUBEand others…
Good to see you here! @Sohail Keegan Pinto
[9:34 PM, 9/5/2021] Arunan HBSCE: What is your objective? @Arunima SN Nattika Cardamine
[9:38 PM, 9/5/2021] Arunan HBSCE: But fresh larvae and pupae came only after the second fly emerged. Is it not? Can you please tell us why it took so long for the larvae and pupae to emerge, if the mother was the original fly you transferred on 12 August? @Aswathy Suresh CUBE

We have been doing *MOSQUITO breeding sites survey.
*Sample collection by killing MOSQUITO after claptrap.
*Students have set MOSQUITO ovitraps, to identify the types of MOSQUITO breeding in the locality.
@ hina cube kanpur.I thinking that , the one fly that I transferred in to media bottle was not properly gravid at that time. But the fly were laying eggs not fertilised. After few days later observed 2 larvae and 1 pupae. And one new fly observed around 30th August , but I couldn’t found it is male or female. Because the fly were died very fast.

I am suspecting that dead newly fly maybe male fly. Because that time I observed only transferred fly & newly emerged fly There could be possible that these flies mated that’s why get more larvae & pupae inside the culture currently I guess.

VIDEOS SHARED BY ASWATHY AT 1.26AM
Fruitfly updates

25th Day : ~18 Flies, ~10 Larvae, ~90 Pupae.

SLBK B21

Transfer Flies Date : 12.08.2021
Transfer Flies No : 12

06/09/2021
Today observation

No of Flies : ~18
No of Larvae : ~10
No of Pupae : ~90
No of Dead Flies : ~30

Further plan
Make new media and transfer flies from current bottle to new bottle.

Update : 06/09/2021
Home Lab : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
Fruitfly updates

25th Day : ~35 Flies, ~20 Larvae, ~120 Pupae.

SLBK E17

Transfer Fly Date : 12.08.2021
Transfer Fly No : 14 Flies
Gravid Flies : 6 ; Male Flies : 8

06/09/2021
Today observation

No of Flies : ~35
No of Larvae : ~20
No of Pupae : ~120
No of Dead Flies : ~40

Further plan
Make new TRSV media , transfer flies from this bottle to new media bottle in order to maintain E culture.

Update : 06/09/2021
Home Lab : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.

Pan-pan, Pan-pan, Pan-pan
TRSV Media for Fruitflies, failed at ReMan’s Lab

Fruitflies Update
ReMan’s Lab Ranchi
06-09-2021

Due to liquification of TRSV media since 4th of September, emergency transfer of flies had done into new culture bottle RNSL-B2

Media preparation date- 05-09-2021
Transfer date- 06-09-2021
No. of fruitflies transferred- 6
Fruitflies with bulgy and pointed abdomen- 2

@Aswathy Suresh CUBE @Yash Sheregare CUBE @Rechel Turkey Cube Ranchi Moina and others.

Fruitflies on Corn! Date:- 6th Sept 2021. Time:- 10.36AM. Cube Locality Lab Ulwe, Navi Mumbai. Data collector:- Abhijeet Singh.

Caught 11 mosquitoes yesterday night as I was awake till 3am … Date- 5sept Time - 11pm-3am… No of mosquitoes- 11… No.of aedes- 0 Data collecter- Gaurangi Dhuri … Location - Tilak nagar, Mumbai
@Gaurangi Dhuri please can we get a clear close up of the mosquito showing its body features in more detail ,one mosquito at a time
Yess… I shall click one of the mosquito’s close up pic

Hello everyone

The broad guidelines of the presentations are out now. The time duration of presentation is 20min and there will be a discussion round after the presentation which is of 15 min for each presentation.

Take us to your home lab in this CUBE NATIONAL MEET-2021, hope to see everyone to start preparing for the presentation.

We can also meet in CUBE chatShaala (https://webinar.hbcse.tifr.res.in/b/kir-hye-cn4) to create connection in model systems.

Be ready to rock.

For more information kindly reply to this message or you can also message us personaly.

Thanks and All the very best👍

[KiranKalakotiR (Kiran Kalakoti)]

6th September 2021, 9.30pm to 7th September 2021, 9.30pm

Mango tree : 1
𝗦𝘁𝗮𝗿𝘁𝘀 𝘁𝗼 𝗳𝗹𝗼𝘄𝗲𝗿𝗶𝗻𝗴
Flowering is observed in many branches of the mango tree.
Date: 6/9/2021.
Time: 12:23pm.
Temperature : 29°C/24°C
Location : Pazhuvil ,Thrissur,Kerala.
Latitude : 10.419129.
Longitude : 76.1522749.
Data collector: Seethalakshmi
1​:partying_face: Mangoes started flowering!

Mango flowers in full bloom Location :Tenkasi Tamilnadu Date 6.9.2021 Data Collector :Binumol & Shrudhiga

Date:6/9/21.Time 4 pm.objective: To identify the cardamine species. Cardamine with developing seed pods observed.CUBE home lab Kozhikode Kerala

Snail passing by road of HBCSE campus. Location: CUBE LAB Mumbai, Date of photo: 05/09/21

Snail at 4th Floor residence, Bhulabhai Desai Road, Mumbai 6 September 2021 Photo: mcarunan

Giant African snail Achatina fullica Cube Ghatkopar

Colour: cream
Location :Pazhuvil
Date: 30/5/21
Time: 1:30 pm
Size:~1cm
Data collector :Seethalakshm

Snail, at 4th Floor residence, Bhulabhai Desai Road, Mumbai 6 September 2021 Photo: mcarunan Looks surely different from the Giant African Snail in looks and size. Looking for comments.

VIDEO SHARED BY JANHVI AT 7.51PM

How is this different from the Giant African Snail? Snail at 4th Floor residence, Bhulabhai Desai Road, Mumbai 6 September 2021 Photo: mcarunan
iRAM:

Today I have trapped 23 earthworm and in one cup culture I have put 2 earthworm I have found then under the flower pots
This is worms
Yes
How many worms you have @~Iram
I have 23 earthworm
Iram, wonderful please join the CUBE Chatshaala by clicking http://hbcse.tifr.res.in/b/kir-hye-cn4
Hello @KIRAN CUBE can you please share today’s snapshot of snail whorls?
First of all, this snail doesn’t have a conical shell like Giant african snail.
And it has broad yellow stripes on the shell where as Giant african snail majorly has brown color and light yellow stripes
Now that’s new !! Cornflies??? Haha