21st August 2021, 9.30pm to 22nd August 2021, 9.30pm
But I got complete life cycle of Psyche butterflies on Cardamine plants.
So we did survey in whole HBCSE campus for Cleome or Capparis & for Cardamine plants
& we get only 3 Cleome plants in whole Campus (at besides G6 lab, near BARC Compound ), whereas we observed huge Cardamine forest (more than 1000 plants) in whole Campus. (Mostly at sorrounding to main HBCSE building)
So it was like ecological survey of 3 different plants in HBCSE campus.
So, Questions came in mind-
✓Cleome & Capparis are belongs to Cleomaceae & Capparaceae family resp.
✓Cardamine belongs to Brassicaceae/ Cruciferae family.
1.why female Psyche butterflies laid egg on Cardamine plants?
✓we get an answer that all 3 families are different but they having common origin meaning they are belonging to same Order - Brassicales
The characteristics of Brassicales order is they are rich source of secondary metabolites i.e.glucosinolates
Hence in all 3 plant families members, Glucosinolates is present.
because of more number of Cardamine plants compare to Cleome/Capparis plants in a Campus so for survive female Psyche butterflies choose Cardamine plants for egg laying.
All observations done in HBCSE campus.
Date- during Jan - Feb 2020.
Collaborators during observations- @KIRAN CUBE mam @Yash Sheregare CUBE @Shivam CUBE NEMATODE
Collaborators in India- @drishya @arunima & all others…
Size of the ant. By using reference sclae. Size: 2.3-3mm.
Time:6:56 pm.
Location : Pazhuvil.
Date:20/8/21.
Data collector : Seethalakshmi. C.R.
Yes sir . They have
VIDEO SHARED BY SEETHALAKSHMI AT 1.43PM
@Arunan HBSCE sir
They have the white bands
Location: Pazhuvil
Date: 20/8/21
Time:7:09 pm
Data collector : Seethalakshmi
Wonderful explanation @Shraddha Sonavane CUBE
Glucosinolates acts like antibiotics so it might help to protect egg from any fungal or bacterial infection
Update on Ant Model system
Location :Pazhuvil
Time:5:27pm
Date :20/8/21 , Data collector : Seethalakshmi
The size of leaves of Cleome and Capparis can also be one of the factor Shraddha
Plant kept in LED for one month and shifted to normal conditions,shows delayed opening of leaves
Plant s under normal conditions
[8:40 AM, 8/21/2021] Shraddha Sonavane CUBE: Thank you 
@Ankit Yadav Xms Work Shop Cube Patkar College
Yes. Glucosinolates help in identification of host plants & as you said it also help in protection of next generations.
It means female butterflies not only select food for next generations but also protect themselves from microbial infections and other predators attacks.
Glucosinolates are slightly bitter in taste so, many organism not prefer glucosinolates containg food.
this is winning situation:partying_face:
for Psyche butterflies.
Because less competition for food and When Caterpillar eat these glucosinolates containg food it might chances that in their guts some amount of glucosinolates present so probably less chance of predators attacks.
[8:45 AM, 8/21/2021] Shraddha Sonavane CUBE: Sorry mam I didn’t get.
But You mean size of leaves of Cleome & Capparis is larger than Cardamine is it?
@Sunita Chahar CUBE mam
[8:53 AM, 8/21/2021] Shraddha Sonavane CUBE: Congratulations @Sunita Chahar CUBE mam 
plant are still surviving in stress conditions for more than one month.
[8:53 AM, 8/21/2021] Shraddha Sonavane CUBE: How many minutes/hours of delayed?
What’s leaves opening time in Control plants and in shifted plant?
I could see some white cottony structure on branches. What are they?
Is it mealy bug attack or something else?
[9:17 AM, 8/21/2021] Abhijeet Singh: Okay, this was new for me!!!
Does butterfly actually select the plant to lay eggs?
@Shraddha Sonavane CUBE @KIRAN CUBE
[9:19 AM, 8/21/2021] Abhijeet Singh: If you have photos please send it @Shraddha Sonavane CUBE !!!
@Hina Mudgal Kanpur CUBE:innocent: Looking for updates on Mosquito studies from CUBE Kanpur to inspire others.
Not convincing evidence. @Batul Pipewala Kolkata Please give references from Wormbook showing how O.tipulae looks. @Anshu Kadam (Nematodes) please collaborate.
So @Sunita Chahar CUBE Ma’am before keeping the plant in LED what was the pattern in opening of leaves and what’s the delay that you are referring to after one Month!?
That’s something very interesting @Sunita Chahar CUBE Ma’am. Please let us know more about this with context!!
Excellent collections from CUBE Adarsha Vidylaya collection @Abhijeet Singh No names of Collaborators?
@Abhijeet Singh
GREAT! @Sidhy Kerala Drosophila
Please post daily updates and make comments on collaborators’ posts, to move forward collectively. @Sidhy Kerala Drosophila @Aswathy Suresh CUBE @Yash Sheregare CUBE @Aastha Ahuja Cube and others
CONGRATS! @Sidhy Kerala Drosophila
Yes. female butterflies sense plant with help of Chemoreceptors. These Chemoreceptors are present on their legs
[9:39 AM, 8/21/2021] Arunan HBSCE: Where exactly this was located sp that you can warn others to look for such habitat spots of Mosquito breeding? @Hina Mudgal Kanpur CUBE Please take initiative to get more collaborators in this nationally important CUBE Public Health Research.
@Hina Mudgal Kanpur CUBE @Abhijeet Singh Right time to prevent mosquito breeding across the country.
[9:46 AM, 8/21/2021] Arunan HBSCE: What is the objective of Cubist Salman Mustafa and his collaborators? @Hina Mudgal Kanpur CUBE
What is your comment? @Abhijeet Singh from your school cubists experience?
[9:47 AM, 8/21/2021] Arunan HBSCE: Comments please @Ishita Assam @Abhijeet Singh @Sachin Pradhan CUBE
[9:49 AM, 8/21/2021] Arunan HBSCE: GREAT! @Shraddha Sonavane CUBE Who were your collaborators in Cardamine- Psyche butterfly interaction research? @Shraddha Sonavane CUBE
[9:50 AM, 8/21/2021] Arunan HBSCE: Please list the names and centers of your collaborators @Shraddha Sonavane CUBE
[9:52 AM, 8/21/2021] Arunan HBSCE: Please make comments @Ishita Assam @Aswathy
@Aswathy Suresh CUBE @Seethalakshmy CUBE and others
[9:55 AM, 8/21/2021] Arunan HBSCE: Have you found locations near your residence where we can expect to find Earthworms, for developing model systems for Spinal cord regeneration studies? @Aswathy Suresh CUBE @Sidhy Kerala Drosophila @Ishita Assam @Abhijeet Singh @Seethalakshmy CUBE
[9:57 AM, 8/21/2021] Arunan HBSCE: Can cellulose in paper alone be sufficient nutrient source, for maintaining Earthworm culture? @Abhijeet Singh @Ishita Assam @Aswathy Suresh CUBE @Sidhy Kerala Drosophila
[10:08 AM, 8/21/2021] Arunan HBSCE: Do the Earthworms eat paper and defecate in the same cup? Would it become like Vermicompost, in the culture cups? @Abhijeet Singh @Aditiya Joshi CUBE @Ishita Assam @Sachin Pradhan CUBE
[10:11 AM, 8/21/2021] Arunan HBSCE: Photos please of Cardamine & Cleome sites as evidence @Shraddha Sonavane CUBE
Great write up @Shraddha Sonavane CUBE
I think it will help to get all life cycle of Psyche butterflies on Cardamine plant *Cardamine* plant: New host plant of Psyche butterflies(Leptosia nina)
What are Glucosinolates? @Shraddha Sonavane CUBE @KIRAN CUBE
Can you comment on the achievement by the addition of a single step in the method while posting photos of ants? @Seethalakshmy CUBE
Do you still think these are Crazy Black Ants? Which Genus and species? @Seethalakshmy CUBE
Collaborators - @KIRAN CUBE mam @Yash Sheregare CUBE @Shivam CUBE NEMATODE
[10:18 AM, 8/21/2021] Arunan HBSCE: Any reference to convince all that these are the Black Crazy Ants? @Seethalakshmy CUBE
How do workers differ from male ants of this species? @Seethalakshmy CUBE
[10:19 AM, 8/21/2021] Arunan HBSCE: Any reference? @Ankit Yadav Xms Work Shop Cube Patkar College
[10:20 AM, 8/21/2021] Arunan HBSCE: Please mention your objectives with each such post for others to take interestin your work @Seethalakshmy CUBE
[10:21 AM, 8/21/2021] Arunan HBSCE: Please clarify @Sunita Chahar CUBE What is your suggestion?
[10:23 AM, 8/21/2021] Arunan HBSCE: Please give details for others to understand and to get inspired and to make comments. @Sunita Chahar CUBE
[10:24 AM, 8/21/2021] Arunan HBSCE: Time of opening and closing please, of each.
And please state the objective of this work.@Sunita Chahar CUBE
Collaborators In India- Drishya from SN college Nattika , Arunima from Kozhikode, Kerala ,@batul mam from Chandani, Kolkatta ,West Bengal.
Wormbook comparision of O tipulae is a drawing whereas I have given a pic from wormatlas showing a light micrograph and it is matching please comment @Arunan HBSCE
[10:25 AM, 8/21/2021] Arunan HBSCE: Reference please @Shraddha Sonavane CUBE
[10:27 AM, 8/21/2021] Arunan HBSCE: Yes, kindly articulate your comments clearly, so that all can understand and thereby extend the conversation @Sunita Chahar CUBE
[10:29 AM, 8/21/2021] Arunan HBSCE: What exactly did you understand from the posts of @Sunita Chahar CUBE on Phyllanthus @Shraddha Sonavane CUBE
Please summarise what you understood. Let’s have clearer discussion. @Shraddha Sonavane CUBE your participation is very effective.
[10:31 AM, 8/21/2021] Arunan HBSCE: Reference please @Shraddha Sonavane CUBE
[10:34 AM, 8/21/2021] Arunan HBSCE: Excellent! @Shraddha Sonavane CUBE
Don’t you think we should further confirm from other centers, though collaborators? @Shraddha Sonavane CUBE
[10:35 AM, 8/21/2021] Arunan HBSCE: Please mention their contribution in this work @Shraddha Sonavane CUBE
[10:36 AM, 8/21/2021] Arunan HBSCE: Pictures please repost with references for all to make comments @Batul Pipewala Kolkata
Referencehttps://www.wormatlas.org/albertson_1976/Albertson_1976.html
VIDEO SHARED BY BATUL AT 11.28AM
As given in the reference a is the buccal cavity, b is the pro corpus, c is the meta corpus and d is the terminal bulb.video taken under 45X of compound microscope. Cube Homelab Kolkata BatulP 20/8/21
BREAKING NEWS The pharynx structure of my soil nematode looks v much like Caenorhabditis elegans from the reference given above.please comment @Anshu Kadam (Nematodes) @
@Arunan HBSCE @Ishita Assam @Shahin🍁 video from Homelab Kolkata BatulP 20/8/21h
[11:35 AM, 8/21/2021] Lakshmy PJ CUBE: This cardamine plants brought from plant nursary. @KIRAN CUBE
I dont know i think it may be cardamine hirsuta or cardamine flexuosa
[11:35 AM, 8/21/2021] Lakshmy PJ CUBE: Thrissur, Mampilly
[11:37 AM, 8/21/2021] Lakshmy PJ CUBE: I expect within 40-50days. There will be first floral bud.
[11:39 AM, 8/21/2021] Lakshmy PJ CUBE: There is gene called KNOX gene respondsible in formation of compound leaves in cardamine. @Arunan HBSCE @KIRAN CUBE @Shraddha Sonavane CUBE
There is one major difference between Starch and Cellulose.
For starch, glucose repeat units are located in the same direction, and each successive glucose unit is rotated 180 degrees in cellulose.
Cellulose is thicker than sugar, which is water-insoluble. On the other hand, the Starch is less weak than cellulose and can be dissolved in warm water.
@Arunan HBSCE sir, @Aswathy Suresh CUBE, @Aditiya Joshi CUBE, @Abhijeet Singh
And others
[12:36 PM, 8/21/2021] Arunan HBSCE: On what basis we can say this? @Lakshmy PJ CUBE How are Cardamine hirsuta and Cardamine flexuosa simar and different from other Cardamines? @Lakshmy PJ CUBE
Don’t we have any reference on Indian Cardamine species?@Lakshmy PJ CUBE
[12:39 PM, 8/21/2021] Arunan HBSCE: How does Knox gene result in formation of compound leaves? @Lakshmy PJ CUBE Please give some relevant references and say what do you understand.
[12:40 PM, 8/21/2021] Arunan HBSCE: Can’t we get better reference than that of coaching classes? @Seethalakshmy CUBE
Let’s all learn together. @Seethalakshmy CUBE
Cellulose is a linear polymer and starch is consist of linear and branches. These arrangement do effect the properties.
The reason is due to the different types of bonding between cellulose and starch. Cellulose has beta-1,4 bonds that are not digested by our enzymes (which can digest alfa-1,4 and alfa-1,6 bonds that are present in starch and glycogen). However, some bacteria from the gastrointestinal tract of human and other animal species can use cellulose as an energy source since they are able to break the beta 1,4 bonds. Actually, that is one of the concepts of fiber (carbohydrates that are not digested by the enzymes produced by the animal, being fermented by bacteria).
The arrangement of the glucose units in starch also differs between amylose (linear structure with alfa-1,4 bonds) and amylopectin (branched structure with alfa-1,6 bonds), explaining the differences in glucose absorption between ingredients with different proportions of these two polymers.
VIDEOS SHARED BY ASWATHY AT 1.17PM
SLBK B 21
[single line 22ndsubculture bottle of B line (species).]
Update : 21 August 2021 ; 1:04pm
Date of transfer flies : 12.08.2021
Transfer no of flies : 12 Flies.
Gravid Flies : 5, Male Flies : 7
Expectations on 9th Day
21/08/2021
~60 Larvae
~60 Pupae
~40 Flies (including new flies)
Observations on 9th Day
21/08/2021
~ 20 Larvae
~ 70 Pupae
~35 Flies (including new flies)
Update : Aswathy Suresh CUBE SN College Nattika.
Home Lab : Bottukadavu - Thrissur Kerala.
SLBK E17
[single line 18thsubculture bottle of E line (species)]
Update : 21 August 2021 ; 1:12pm
Date of transfer flies : 12.08.2021
Transfer no of flies : 14 Flies.
Gravid Flies : 6, Male Flies : 8
Expectations :
21.08.2021 : 9th Day
~80 Eggs
~50 Larvae
~40 Pupae
Observation : 9th Day
21/08/2021
No of Eggs : 50
No of Larvae : 20
No of Pupae : 20
No of Flies : ~10 (Including newly emerged flies).
Dead Flies : 5
Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
We baked bread today. This cloth was used to cover the dough while kept for rising. Smell of Yeast on the fermenting dough may have attracted Fruitflies to this piece of cloth. 20 August 2021 CUBE HomeLab Sophia, Bhulabhai Desai Road, Mumbai Photo: mcarunan
SLBK A 22
[single line 23rdsubculture bottle of A line (species).]
Update : 21 August 2021 ;1:21pm
Date of transfer flies : 12.08.2021
Transfer no of flies : 1 (Gravid)
Expectations on 9th Day
21/08/2021
~50 Eggs
~20 Larvae
~10 Pupae
~40 Flies (including new flies)
Observations on 9th Day
21/08/2021
~20 Eggs
~ 3 Larvae
~ 0 Pupae
1 Fly (Transferred)
Update : Aswathy Suresh CUBE SN College Nattika.
Home Lab : Bottukadavu - Thrissur Kerala.
SLBK E15
Update : Fruit Flies
subculture started date : 02.07.2021
Transfer Flies : 15 Flies
Gravid Flies : 8, Male Flies : 7
Expectations :
21.08.2021 : 50 Days old culture bottle
~200 Eggs
~60 Larvae
~100 Pupae
Observation : 50th Day
21/08/2021
No of Larvae : ~10
No of Pupae : ~200
No of Unhatched Pupae : ~70
No of Flies : ~10
Dead Flies : ~70+
Media solidified
Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
SLBK E16
Update : Fruit Flies
subculture started date : 19.07.2021
Transfer Flies : 30 Flies
Gravid Flies : 9, Male Flies : 21
Expectations :
21.08.2021 : 34th Day
(More than one month old culture)
~350 Eggs
~80 Larvae
~160 Pupae
~100 Flies
Observation : 34th Day
21/08/2021
No of Eggs : ~150+
No of Larvae : ~30
No of Pupae : ~200+
No of Unhatched Pupae : ~40+
No of Flies : ~70+
Dead Flies : 80+
Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
[1:45 PM, 8/21/2021] Arunan HBSCE: Please post separate ose up, photos showing larvae and eggs in this Bottle SLBK A22, as clear evidence. @Aswathy Suresh CUBE
[1:46 PM, 8/21/2021] Arunan HBSCE: Close* up
Most of eggs present inside the media, ~100 more eggs observed in E16 bottles. Picture taken from SLBK E16. Date of picture 2:43pm.
SLBK A22 - 1st instar larvae found inside culture bottle, picture taken on 21/08/2021: 2:53pm
Okay
Any update on this now?
Nice!
How do you make sure that there are still new flies emerging in this bottle?
What are your plans with these cultures?
[3:27 PM, 8/21/2021] Lydia cUBe Drosphila: This is not that old bottle. How long does a culture bottle survive on average?
And what could be the reason for the death of so many flies? What do you think?
[3:31 PM, 8/21/2021] Lydia cUBe Drosphila: Yes understood
Why not explain using some examples? Some relevant examples of proteins…a protein that can reverse back to the original structure and another protein that is irreversible…a comparison kind of.
By this, we’ll always remember such examples.
[3:33 PM, 8/21/2021] Lydia cUBe Drosphila: How does a non-clubbed antenna look?
Can you please send some pictures of the ants 
with these characteristics that you think are morphological features.
Within 5 days, 10 flies dead!
Were those flies too old when you transferred?
[3:36 PM, 8/21/2021] Lydia cUBe Drosphila: What’s the difference between these 2 kinds of ants?
[3:39 PM, 8/21/2021] Lydia cUBe Drosphila: Are those white stripes on aedes mosquitoes 
legs quite visible?
[3:44 PM, 8/21/2021] Lydia cUBe Drosphila: True!
Got it
I got some doubts from some of my friends… I wasn’t able to convince them completely w.r.t. organism and system…so thought of asking.
Thank you!
[3:44 PM, 8/21/2021] Lydia cUBe Drosphila: Yes exactly!
We’ll get a context to get to know more on these curriculum topics!
[3:52 PM, 8/21/2021] Lydia cUBe Drosphila: Egg yolk too contains proteins right?
So same is the case for both egg whites and egg yolk…right?
[3:55 PM, 8/21/2021] Lydia cUBe Drosphila: Would the presence of fats interfere with the protein denaturation process?
[3:59 PM, 8/21/2021] Lydia cUBe Drosphila: Yes Sir!
True!
A healthy culture bottle will give us all the stages of a fruit fly! We do expect to see eggs, larvae, pupae and adult flies!
More interested to see the larva, as an indicator that fertilized eggs hatch to larvae.
Please post larvae of SLBK A22.
How are we sure this is 1st instar larva? @Aswathy Suresh CUBE
Kindly click on the above👆🏻 given link for the summary of last discussion.
Invitation to join today’s CUBE chatShaala, 
Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in all CUBE what’s app groups and on metastudio after the session
Here’s the direct link of the CUBE webinar: https://webinar.hbcse.tifr.res.in/b/kir-hye-cn4
Note: we have changed the link to avoid technical error
Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
INVITATION TO JOIN TODAY’S CUBE CHATSHAALA
Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in CUBE what’s app groups after the session
Webinar Link :
Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
Attention!
Owing to unexpected technical issues regarding the regular Big Blue Button Platform where the CUBE Chatshaala is held everyday, we are shifting (temporarily, for today) to
Jitsi.
In case of any issues, please let us know in the group or on personal.
VIDEOS SHARED BT BATUL AT 6.21 PM
BREAKING NEWS The pharynx structure of my soil nematode looks v much like Caenorhabditis elegans from the reference given above.please comment
As given in the reference a is the buccal cavity, b is the pro corpus, c is the meta corpus and d is the terminal bulb.video taken under 45X of compound microscope. Cube Homelab Kolkata BatulP 20/8/21
Wow
Summary of CUBE Chatshaala(21/8/21)
Discussed about *Circadian rythm( sleep wake cycle )
vs
Circanual rhythm.( mango fruiting )
-
What is Altitude and Longitude? How can define?
-
Why it is Important in Mango maping?
-
Mango starts to Flowering at first in which region ( North or South)?
As well as which month??
Others please give your inputs
@Arunan HBSCE sir, @Aswathy Suresh CUBE, @Abhijeet Singh, @Saida Sayyed
And others
[12:14 AM, 8/22/2021] Shraddha Sonavane CUBE: Yes Sir, It will be really great if @Arunima SN Nattika Cardamine @Drishya share their work on Butterflies & Cardamine interaction in their Home labs. @Arunima SN Nattika Cardamine & @Drishya can you please share your work on Butterflies & Cardamine interaction
[12:16 AM, 8/22/2021] Shraddha Sonavane CUBE: because of collaborations only, we could provide Nectar for newly emerged Psyche butterflies by providing Sugar solution with help of cotton & Vernonia plants as Nectar plant.
Summary of 520th day of Cube Chatshaala
Discussion started with @batul Ma’am’s nematodes culturing and identification.
Currently batul Ma’am culturing nematodes on potato slices. With that she is trying to identify C.elegans from her nematodes if there’s any in her culture!
Identification:-
So for identification of C.elegans she is using a photo of C.elegans from a research paper(The Pharynx of Caenorhabditis elegans Albertson 1975) and compare the morphological characteristics with the nematodes that she has cultured in her homelab!
Identification so far:-
So far she is able to compare the Anterior part(i.e. pharynx).
After that discussion went on about the speculation and casual observation that we make in our experiments and our interpretation of that! With reference to Aswathy saw Nematodes after 15days in her drosophila TRSV media which has vinegar and Batul Ma’am is not able to culture nematodes with RSV media with has Vinegar.
A very important point that came after discussion is that:-
Speculation, Casual observation is always welcomed!! but it is to be verified with evidence and experimental design with enough replicates and control
This leads us to the very fundamental idea of CUBE and ofcourse of science is:- CONSTRUCTION AND NOT INSTRUCTION
This knowledge construction can be through 2 types of collaborators:-
1)Involved in Discussion
2)Working on same model organism
Moving forward with Discussion on Circadian Rhythm and understanding it properly. A circadian rhythm or circadian cycle, is a natural, biological process that regulates and repeats roughly every 24 hours. … The term circadian comes from the Latin circa, meaning “around” (or “approximately”), and diēm, meaning “day”.(example:- opening and closing patter of leafs in phyllanthus)
Also Circannual Rhythm. The term circannual is Latin, circa meaning approximately and annual relating to one year.(example mango flowering)
Moving forward with discussing the CIRCADIAN RHYTHM with respect to stomatal opening and closing in plant’s leafs and photosynthesis process(with context to secondary school biology curriculum) .
AND
CIRCANNUAL RHYTHM with respect to mango Flowering and Fruiting pattern throughout India.
3days after inoculation of fermented water. Slices in red marked outline are inoculated with fermented coconut water from closed bottle. White cottony colonies, Greenish n blackish colonies are seen. Also, circular whitish colonies can be seen too. Also few small thread like colonies too. Marked blue are slices inoculated with fermented coconut water from open bottle. Thread like colonies are seen. Also blackish n white cottony growth are seen… Thread like colonies are seen on control slices. The color of these thread like colonies is seen to be changed. 21/8/2021. Kala Talav, Kalyan west.
There is a nasty smell… Strong smell similar to that of spoilt bread… There were some small flies too
[7:28 AM, 8/22/2021] Abhijeet Singh: How this chemoreceptors help them! @Shraddha Sonavane CUBE
Please elaborate!
[7:39 AM, 8/22/2021] Abhijeet Singh: I think sir! Because we have seen earthworm surving on paper for a complete year!
Humans cannot digest Cellulose and that us why I think we cook our vegetables and then eat!
But earthworm can digest it and survive it.
So cellulose can be the source of nutrition for earthworms to survive!
[7:39 AM, 8/22/2021] Abhijeet Singh: What do you think @Aswathy Suresh CUBE @Aditiya Joshi CUBE @Ishita Assam and others!!?
[7:44 AM, 8/22/2021] Abhijeet Singh: Yes Yes! That’s is very interesting thing sir! I would like to tell this to everyone that, in cube lab, we had a one year old cup culture. Same tissue paper was given to earthworms to feed on. The the tissue paper in the cup was eaten and again eaten and again since one year! So we can literally see that the paper had become very similar to what soil looks like!
I’ll look for photographs from collaborators if I can get I’ll post it in the group!
Koel call
Date : 21.08.2021
Time : 5:46pm
Location : Bottukadavu - Thrissur Kerala.
Data collector : Aswathy Suresh CUBE S N Nattika
How do you compare with diagrams in this reference?
@Batul Pipewala Kolkata @Anshu Kadam (Nematodes) @Saida Sayyed @Aswathy Suresh CUBE @Lydia cUBe Drosphila
What is the difference between alpha & beta 1,4 linkage? @Seethalakshmy CUBE
Any clear diagram to distinguish? @Seethalakshmy CUBE @Abhijeet Singh @Aswathy Suresh CUBE @Aastha Ahuja Cube @Raj Yashwantrao Patil CUBE @Sachin Pradhan CUBE @Ishita Assam
My plan is to transfer these flies in to new media bottles.
Also planing to harvest second instar larvae using these flies in order to perform olfactory assay experiment.
[11:11 AM, 8/22/2021] Arunan HBSCE: @Shraddha Sonavane CUBE What do you think is the work of Drishya and @Arunima SN Nattika Cardamine
[11:12 AM, 8/22/2021] Arunan HBSCE: Please mention the major contribution of each of your collaborators @Shraddha Sonavane CUBE
One healthy culture bottle survive maximum 2 months. After media become liquefied and also flies,larvae,pupae number will decrease inside after 2 months old culture bottles.
Can you tell us first about what you expected in Control slices?
Please give *your interpretation based on these Control slices, after writing subheadings. @Aditiya Joshi CUBE
Transferred flies from B20 bottles which was made on 19.07.2021 after I transferred in to B21-12.08.2021 bottle. ~20 days old flies I transferred from B20 to B21 bottle.
[11:17 AM, 8/22/2021] Arunan HBSCE: Please be clearer in your description starting with Control. @Aditiya Joshi CUBE
[11:20 AM, 8/22/2021] Arunan HBSCE: What nutrition Earthworms can derive from Cellulose? @Abhijeet Singh @Seethalakshmy CUBE @Aswathy Suresh CUBE
What about other nutrients?
[11:24 AM, 8/22/2021] Aswathy Suresh CUBE: Yes sir, This is a zoomed picture of larvae that I taken from SLBK A22. Picture is not much clear, But we can able to see the mouth hook part of this larvae is not much fully developed. 2 black sports kind thing can see at anterior portion. Spiracles is not present at the tip of body. that’s why I concluded this is first instar larvae of fruit flies
[11:32 AM, 8/22/2021] Aswathy Suresh CUBE: Yesterday discussed about Mango flowering in different parts of country based on Latitude & Longitude. Is there any significants chances we can able to see in mango flowering from lower to higher latitude (N) regions.
Also discussed about importance of latitude and longitude marking while in data collection.
Water:cup_with_straw:( 150 ml)
(~50 gm)
Rava:rice:(20 gm)
(15 gm)
Transfer some flies from a single line culture into a empty bottle.
Remove the bottle and we can see flies getting immobilise.
?
species, right 
_agariplaadil..JPG)
and Ask Why tomorrow!
.
@Rechel Turkey Cube Ranchi Moina Please add your summary about your design of experiment, How to study Life span of moina.
In yesterday’s chatshala, we started discussing with the microscopic picture which was shared by 
, like from tap water using nutrient rich coconut water. 
(suspect it to be a Phorid fly)
Even before Autumnal Equinox, lower Latitudes, like Thrissur10.4°N shows Mango trees flowering. 
Please give a summary of your work and of other cubists of Nellore, so far.
At what time we will get maximum and minimum no.of.flies?
wow it’s look to different …
@~Iram !what’s the situation today …you say that they are happy with tissue paper…but you add salt water so what the situation ?..can earthworm lives happily with salt water ?@~Iram
sorry @~Iram
