22nd August 2021, 12.09pm to 23rd August 2021, 9.30pm
Guava …
Please give reference link of larval features of 1st, 2nd and 3rd instar, to say why you think this is 1st instar and hence to convince all. @Aswathy Suresh CUBE
[12:46 PM, 8/22/2021] Arunan HBSCE: Wonderful! @Prashant Kumar Sahu
Please te us the exact Location, Date and Name of Photographer along with each photo. Please repost with essential description @Prashant Kumar Sahu
[12:48 PM, 8/22/2021] Arunan HBSCE: Please start giving your Hypothesis on Mango fruiting with respect to a. Latitudes & b. Longitudes in India @Aswathy Suresh CUBE @Batul Pipewala Kolkata @Saida Sayyed @Abhijeet Singh
[12:48 PM, 8/22/2021] Arunan HBSCE: @Seethalakshmy CUBE
[12:51 PM, 8/22/2021] Arunan HBSCE: Please write about your prediction on Mango flowering in Kanyakumari and Thiruvananthapuram, as discussed yesterday, based on previous experience of Cubists? @Aswathy Suresh CUBE @Abhijeet Singh @Seethalakshmy CUBE
How do Mango trees respond in the 3rd week of August? CUBE HomeLab Sophia, Bhulabhai Desai Road, South Mumbai, Latitude19°N, Longitude79°E 20 August 2020 Photo: mcarunan
[1:39 PM, 8/22/2021] Lydia cUBe Drosphila: Interesting!
What do you think of these observations that you got? Why is there a difference?
One more thing…after inoculating these potato slices, where did you keep them? And for how long?
[1:43 PM, 8/22/2021] Lydia cUBe Drosphila: In what all is cellulose present other than paper?
[1:44 PM, 8/22/2021] Lydia cUBe Drosphila: Looking forward to the same!
[1:46 PM, 8/22/2021] Lydia cUBe Drosphila: Okay
So why not send us your plan in detail.
Tell us, larvae from which culture bottle you are preferring…details of that culture bottle.
Send us your detailed plan for the olfactory assay and of the culture bottles that you’re going to use.
[1:49 PM, 8/22/2021] Lydia cUBe Drosphila: So what’s the story of the B20 bottle? From where did you transfer the flies into B20?
~20 days old flies are not that old, right @Aswathy Suresh CUBE?
Also, what’s the lifespan of a fruit fly? How long can a fruit fly live?
[1:52 PM, 8/22/2021] Lydia cUBe Drosphila: Nice!
Please send pictures of the 3 stages of fruit fly larvae, where we can clearly distinguish between the stages. It will be helpful for you as well as for others.
Also, what are the main features or characteristics that you look for in a larva to identify its stage?
Look for the tiny external head, three thoracic segments, eight abdominal segments, and a telson
extending beyond the anus. These segments are delineated by ventral rows of tiny hooks, denticle
belts, which prevent the larva from slipping backward as it moves forward with waves of extension
and contraction. You should also see a number of sensory bristles in the first instar larva distributed
over the cuticle.
[2:43 PM, 8/22/2021] Shraddha Sonavane CUBE: Sir, I only remembered about @Arunima’s work.she had observed Psyche butterflies larvae in her home lab during lockdown. But I don’t know exactly what was work done by Drishya.
[2:43 PM, 8/22/2021] Shraddha Sonavane CUBE: @KIRAN CUBE mam help in
1.set ups
2.In ecological survey of three different plants in Whole HBCSE campus.
3.during adult butterfly catching from HBCSE garden.
@KIRAN CUBE
@Yash Sheregare CUBE & @Shivam CUBE NEMATODE helped me by asking different questions in causeries, also in observations mainly during when butterflies was emerged from Pupae & in making poster for Science day presentation at TIFR, Mumbai on 29 Feb 2020.
Sun, Aug 29 at 11:00 GMT+05:30 Online Event Redirecting...
A gustatory receptor involved in host plant recognition for oviposition of a swallowtail butterfly
Authors- Katsuhisa Ozaki, Masasuke Ryuda, Ayumi Yamada, Ai Utoguchi, Hiroshi Ishimoto, Delphine Calas, Frédéric Marion-Poll, Teiichi Tanimura & Hiroshi Yoshikawa .
Published: 15 November 2011 https://doi.org/10.1038/ncomms1548
Female butterflies lay eggs on their host only when they detect specific chemicals through their foreleg chemosensilla while drumming on the leaf surface. Here we show that the butterfly, Papilio xuthus, uses a gustatory receptor specific for synephrine to select its host in oviposition behaviour.
@Shraddha Sonavane CUBE Please coordinate with @Arunima SN Nattika Cardamine to tell us more about Cardamine- Psyche butterfly interaction. @Shraddha Sonavane CUBE
ALERT:-
From last year’s data collected by Cubists, we are having a Hypothesis that Mango Flowering start from south Kerala and
Hence, collecting data of what mango trees does now onward is very useful to test the Hypothesis of Cubists.
All cubist are hereby requested to look around for MANGO FLOWERING in their locality and send pictures Of mango tree with NAME OF DATA COLLECTOR, NAME OF LOCALITY LAB, DATE, TIME, LONGITUDE AND LATITUDE, and MINIMUM AND MAXIMUM TEMPERATURE.
Crucial time for Mango Mapping!!
Looking forward for collaboration from all cubists across India!
Comparing with diagrams too there are two bulb like structures in my nematode instead of swollen pharynx please see the whole video
VIDEOS SHARES BY 7.37PM
16day old nematode culture shows good growth of nematodes potato is almost degraded .22/8/21Cubehomelab Kolkata BatulP
Nematodes in subculture B cultured with milk added on first day on raw potato and nematodes transferred from original raw potato slice22/8/21Cubehomelab Kolkata BatulP
Nematodes growing on raw potato slice subcultureC started on 16thAug21.Milk was added once again yesterday to help in growth of nematodes so the surface is looking white.Nematode can be seen on the marked area.22/8/21Cubehomelab Kolkata Batul P
[11:38 PM, 8/22/2021] Aswathy Suresh CUBE: Hypothesis is that lower latitude going upper latitude region there is kind of difference can able see in mango flowering. Based on last year observation came from Thiruvananthapuram - Kerala, 8.5241° N part mango started flowering on september last week onwards. Thrissur - Kerela 10.5276° N started mango flowering observed on October last weeks onwards. Going to upper part like Mumbai 19.0760° N - November/December , Kolkata 22.5726° N its taking December/January (If iam wrong please correct it). This is a kind of pattern is repeating or not in every year (Circannual Rhythm) this is we are addressing questions. We need to check this throughout collecting data from different parts of country.
[11:50 PM, 8/22/2021] Aswathy Suresh CUBE: Kanyakumari is 8.0883° N, 77.5385° E.
Trivandrum is 8.5241° N, 76.9366° E
Kanyakumari & Trivandrum are 8N away from equator (0N). But there is not much drastic change can’t able to see between these of them 8.0N - 8.5N.
Prediction : Kanakumari mango flowering expecting in first week of September onwards. In Trivandrum - mango flowering expecting in last week of September.
Nice picture 
We can able to see some Fruit flies present inside the Guava fruit.
Yes sir! We can search for wet places. Earthworms mostly found in nutrient black soil.
Awesome 
.
Why fruit flies are attracted towards cloth ? Because of smell of yeast ? What are they doing on clothes ?
[12:05 AM, 8/23/2021] Seethalakshmy CUBE: Yes sir! For culturing them cellulose as well as water is enough
[12:07 AM, 8/23/2021] Seethalakshmy CUBE: Sry sir! I didn’t get the question
[12:09 AM, 8/23/2021] Seethalakshmy CUBE: common name: crazy ant
scientific name: Paratrechina longicornis (Latreille) (Insecta: Hymenoptera: Formicidae)
https://entnemdept.ufl.edu/creatures/urban/ants/crazy_ant.htm
The crazy ant is so morphologically distinctive that it is one of the few Paratrechina that is not consistently misidentified in collections (Trager 1984). The crazy ant worker is relatively small (2.3-3 mm). The head, thorax, petiole, and gaster are dark brown to blackish (Creighton, 1950); the body often has faint bluish iridescence. The body has long, coarse, scattered, suberect to erect, grayish or whitish setae (hair-like projections).
And also their rapid movement as well as white bands
Yes we needs more improvement for identifying the genus and species level.
As well as we needs to study it’s morphology
[12:15 AM, 8/23/2021] Saida Sayyed: Breaking news
I lost all my Moina bottle culture 
The tray in which I keep Moina fell over
[12:15 AM, 8/23/2021] Saida Sayyed: Currently, I have 2 tanks culture
30 Moina in each
Ok sir!
Immediative objective : To culture the Ants
Short term objective : To study it’s morphology.
Long term objective : How do ants communicate
Coordinating Growth and Review Maturation — Insights from Drosophila - PMC.
[12:35 AM, 8/23/2021] Aswathy Suresh CUBE: B20 flies I transferred from B19 Bottle - subculture of B line. Yes ma’am, those flies are not much old flies that’s also freshly got new flies from B19 bottle.
[12:36 AM, 8/23/2021] Aswathy Suresh CUBE: Life Span of Drosophila 70 Days - 90 Days.
[12:37 AM, 8/23/2021] Aswathy Suresh CUBE: A typical, healthy and well-maintained outbred Drosophila population will have a median lifespan of approximately 70 days and maximum of approximately 90 days at 25 °C. At a more detailed level of physiological decline, numerous markers of ageing-related loss of function can be observed.
https://www.sciencedirect.com/science/article/pii/S0925443917303319#:~:text=A%20typical%2C%20healthy%20and%20well,of%20function%20can%20be%20observed.
[7:46 AM, 8/23/2021] Lydia cUBe Drosphila: So by this, what do you think?
Can you please tell the transfer dates of B19, B20 and B21?
By this we can say how old the flies were in bottle B21.
[7:48 AM, 8/23/2021] Lydia cUBe Drosphila: What do you think of this claim? Do you think it’s right? How long does your flies live? Do your flies live for 70-90 days? And how does the lifespan of fruit fly depend on the temperature?
[7:49 AM, 8/23/2021] Lydia cUBe Drosphila: Ohh, how do you know that ants 
communicate?
Is there any particular behavior that we can notice and say that these ants are communicating?
[7:53 AM, 8/23/2021] Lydia cUBe Drosphila: Nice!
Please share the reference link to this picture.
Have you seen or identified these characteristics in the larvae in your culture bottles?
[7:59 AM, 8/23/2021] Lydia cUBe Drosphila: What do you think @Aswathy Suresh CUBE?
What could have happened?
It’s not flowering nor fruiting now!
I’ll try to get as many photos and samples to confirm that not flowering and fruiting is normal with mango trees in Jamshedpur in the month of August!
VIDEOS SHARED BY 10.30AM
*BREAKING NEWS Model organism C elegans can be cultured on raw potato slice with milk added to it on first day.Successfully maintained culture started on 29thJuly21,and subsequent subcultureB started on 6thAug,21 and subcultureC started on 16thAug,21 till date.video below shows nematodes growing in subcultureC today 28/8/21 Cubehomelab Kolkata Batul P
[11:04 AM, 8/23/2021] Aswathy Suresh CUBE: I didn’t felt that fruit flies live for 70-90 days. Because I never seen it in my culture. Also my culture is mixed with young and old flies. And every subcultures bottles after 15 days later can able see ~30 dead flies. This means flies have only ~30days life span, I suspect.
[11:05 AM, 8/23/2021] Aswathy Suresh CUBE: 
B19 - Transfer date 02.07.2021
B20 - Transfer date 19.07.2021
B21 - Transfer date 12.08.2021
[11:08 AM, 8/23/2021] Aswathy Suresh CUBE: I think the smell of fermented yeast, attracted these flies towards clothes.
[11:49 AM, 8/23/2021] Abhijeet Singh: Is there a story behind expecting approx, 50 eggs and larve and pupae and ~40 flies on the 9th day?
What was your idea behind your expectations!?
@Aswathy Suresh CUBE
[11:53 AM, 8/23/2021] Abhijeet Singh: Yes we can!!
With the help of blackpaper as background it’s clearly visible!!
I’ve posted the photos too!
Please follow the thread to see the photos.
If you don’t find it I’ll send it again!!
[12:05 PM, 8/23/2021] Abhijeet Singh: Cellulose is mainly used to produce paperboard and paper. Smaller quantities are converted into a wide variety of derivative products such as cellophane and rayon.
Cellulose for industrial use is mainly obtained from wood pulp and cotton.
Some animals, particularly ruminants and termites, can digest cellulose with the help of symbiotic micro-organisms that live in their guts, such as Trichonympha. In human nutrition, cellulose is a non-digestible constituent of insoluble dietary fiber, acting as a hydrophilic bulking agent for feces and potentially aiding in defecation.
Reference:-
VIDEOS SHARED BY YASH 12.48PM
Today’s Headline - 16th Day update of SLPbA2 sub culture shows 12 fruit flies, few larvae, pupae and plenty of dead fruit flies!
Media in SlPbA2 is liquidy as compared to SLPbB2 and C2!
Media date - 06/08/2021
Transfer date - 7/08/2021
No of flies transferred on 7/08/2021 - 35
Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality
23/08/2021
No of flies -12
No of larvae - 12- 3mm larvae, 5 - 2mm larvae
No of pupae - 20
No of dead flies - 30 @Aswathy Suresh CUBE @Arunan HBSCE sir @Lydia cUBe Drosphila @Isha Pawle and others…
Today’s Headline - 16th Day update of SLPbB2 sub culture shows 40 fruit flies and plenty of larvae and pupae.
Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality
Media date - 06/08/2021
Transfer date - 7/08/2021
No of flies transferred - 30
16/08/2021
No of flies - 40
No of larvae - 10 - 3mm larvae, 5- 2mm larvae, 10 - 1mm larvae
No of pupae - 40 unhatched pupae
No of dead flies - 20
Today’s Headline - 16th Day update of SLPbC2 sub culture shows 50+ fruit flies and plenty of larvae of all stages and pupae.
Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality
Media date - 06/08/2021
Transfer date - 7/08/2021
No of flies transferred - 30
23/08/2021
No of flies - 50+
No of larvae - 10 - 3mm larvae, 15- 2mm larvae, 10 - 1mm larvae
No of pupae - 10 unhatched pupae
No of dead flies - 10-15
Wow! @Aswathy Suresh CUBE Congratulations!
Did we just save the A line culture by transferring the gravid fruit fly to new media bottle!?
@Lydia cUBe Drosphila @KIRAN CUBE
We remember there were no larvae in A21 culture 10 days back and now there are plenty of eggs and larvae in new A22 sub culture!
PubMed Central Image Viewer. WoW! This nematode cultured by @Batul Pipewala Kolkata maam looks like C elegans according to the reference above! @Anshu Kadam (Nematodes) @Shivam CUBE NEMATODE what do you think?
Mango tree not started flowering!
Flowering not started
No sprouting
No Fruiting Yet.
Date : 23.08.2021,
Time : 12:17 PM
Latitude: 10° N Longitude: 75° E
Temperature :
Maximum : 32°C
Minimum: 28°C
Location: Ponnani, Malappuram, Kerela
CUBE Homelab Ponnani.
Data Collector: Amal kallingal
Sir I’m expecting flowering to start by September.
I’ll keep getting updates and I’ll add the observation in the given link!!
This is my expecting from one gravid fly that transferred from A21 to A22 after 9th day.
One gravid fly lay 50 eggs per day (Referance says) . In this calculation we will get 450 eggs on 9th day. Also get ~50-60 progeny’s get in 9th days.
But usual observation eggs are present inside cultures ~20-40 is maximum can able see but it fertilized or have not no idea because Larvae present in culture very less.[3:07 PM, 8/23/2021] Aswathy Suresh CUBE: Yes @Yash Sheregare CUBE, Eggs Can able to see inside the culture ~20-40, But larvae are very less present culture. Only 3 larvae I can able to see inside culture.
[3:07 PM, 8/23/2021] Aswathy Suresh CUBE: Expecting some more larvae will come out from these eggs.
How many days old culture bottle is this @Yash Sheregare CUBE?
When do you expecting pupae from this bottle? @Man Masih Beck RANCHI CUBE
[3:27 PM, 8/23/2021] DP HBCSE:
[3:28 PM, 8/23/2021] DP HBCSE: Some have already started! Is this to the South of your place @Abhijeet Singh?
Expecting pupae on 26th Aug(7th day of transfer)
butterfly (psyche )can connect with Cardamine
Great capture @Batul Pipewala Kolkata ma’am
But I still feel that the part which you are claiming to be 1st bulb, doesn’t look like a bulb to me.
@Yash Sheregare CUBE @Arunan HBSCE @KIRAN CUBE and others
If you see the whole video rather than still photo you may be able to see a bulb rather than swollen pharynx
Pruitfly on Apple piece in wine glass at CUBE HomeLab Bhulabhai Desai Road, Mumbai 23/8/08/2021 Photo: mcarunan
VIDEO SHARED BY SEETHALAKSHMI AT 6.51AM
Phone microscopy to see nematodes on a glass slide using flash below
Nematodes moving on potato surface caught by mobile camera and a magnifying glass
VIDEOS SHARED BY BATUL AT 9.11PM
Picking up a nematode to transfer to another potato slice
Water:cup_with_straw:( 150 ml)
(~50 gm)
Rava:rice:(20 gm)
(15 gm)
Transfer some flies from a single line culture into a empty bottle.
Remove the bottle and we can see flies getting immobilise.
Here’s the direct link of the CUBE webinar: 
?
species, right 
_agariplaadil..JPG)
and Ask Why tomorrow!
.
@Rechel Turkey Cube Ranchi Moina Please add your summary about your design of experiment, How to study Life span of moina.
In yesterday’s chatshala, we started discussing with the microscopic picture which was shared by 
, like from tap water using nutrient rich coconut water. 
(suspect it to be a Phorid fly)
Even before Autumnal Equinox, lower Latitudes, like Thrissur10.4°N shows Mango trees flowering. 
Please give a summary of your work and of other cubists of Nellore, so far.
At what time we will get maximum and minimum no.of.flies?
wow it’s look to different …
@~Iram !what’s the situation today …you say that they are happy with tissue paper…but you add salt water so what the situation ?..can earthworm lives happily with salt water ?@~Iram
sorry @~Iram
