Archives of CUBE Context 2 Curriculum what's app group discussion

[KiranKalakotiR (Kiran Kalakoti)]

Date: 01st August 2021

[7:50 AM, 8/2/2021] @magpie Nematode moving on rava sugar vinegar medium 10minutes after transfer 1/8/21 Homelab Kolkata Batul
[7:51 AM, 8/2/2021] @magpie No movement of nematodes on the rava sugar vinegar medium 24hrs after transfer, the whole plate has been scanned have they gone deep in the rava or are they dead?
[8:27 AM, 8/2/2021] P. Chitralekha: Yes. Great work Shraddha. How did you provide continuous dark condition?
[9:08 AM, 8/2/2021] @Lydia : As a testable hypothesis, we can state that if adult flies emerge from the trapped pupae kept inside the bottle, then this means that there is no harm in detaching the pupa from its surface onto which it sticks during pupation or that it won’t harm the pupa in the process of development, and hence we can observe its further developmental stages.
[9:17 AM, 8/2/2021] @Lydia : There is a lot of condensation in this bottle @Aswathy Suresh sN nattika Drosophila
What do you do immediately after you pour the medium into the bottle?
Do you allow the media bottle to cool down?
[9:20 AM, 8/2/2021] @Arunan : What are the fifteen stages of pupae, according to the article? @Janhvi Deshmukh
[9:21 AM, 8/2/2021] @Arunan Daily update please! Expectation, Observation along with interpretation, please!
[9:33 AM, 8/2/2021] @Shraddha276 : Thank you😊 @Sunita Chahar ma’am @Batul Pipewala Kolkata @P. Chitralekha
[9:33 AM, 8/2/2021] @Shraddha276 : Yes @Batul Pipewala Kolkata mam leaves opened as well as closed time is most important. I think on basis of that only we can conclude whether 24hrs continuous light & dark exposure affected or not.
[9:34 AM, 8/2/2021] @Arunan : @KIRAN CUBE
[9:34 AM, 8/2/2021] @Shraddha276 :I Kept plants in box like place under cupboard & this covered by another box by placing half inside & half outside.
So it’s helping me to maintain continuous dark conditions & in aeration also.
[9:36 AM, 8/2/2021] @Arunan : Sorry, sent as an appetizer for Kitchen Lab Movement!
Looking for the real ones, from others!
[9:37 AM, 8/2/2021] @Arunan : Any news on Pagalapos studies? @Lydia
@Hina Mudgal @Lakshmi PJ @KIRAN CUBE @P. Chitralekha@Aditya Joshi
[9:38 AM, 8/2/2021] @Arunan : Looking for Monsoon Fast Plants @Shraddha@Yash @Lakshmi PJ @KIRAN CUBE and others.
[9:42 AM, 8/2/2021] @Arunan : @Lydia Please give a design stepwise, so that all of us can test your Hypothesis, in our HomeLabs
[9:44 AM, 8/2/2021] Arunan: Good question @Lydia cUBe Drosphila
Can @Aswathy Suresh @Aastha Ahuja @Janhvi Deshmukh comment?

What is the comment by @Lydia ?
[9:46 AM, 8/2/2021] Batul Pipewala : Are there two set ups 1 and 2?How are they different
[9:46 AM, 8/2/2021] P. Chitralekha:
[9:46 AM, 8/2/2021] Anshu Kadam: @Arunan sir I’m not sure in which soil sample we will find C. elegans. But according to our previous findings and observations, we found Caenorhabditis genus nematodes in soil sample of Jamun tree of Ratnam College, Bhandup and Almond tree in HBCSE surroundings.
@Batul Pipewala which soil sample are you using? And if possible for you, then try getting soil sample from some fruiting trees!!!
[9:47 AM, 8/2/2021] Arunan HBSCE: Any one has Mango trees in their locality in sprouting, flowering or fruiting in the First week of August?
@Lakshmi PJ @Dr. Nisy Prasad @Shrudhiga @KIRAN CUBE @P. Chitralekha Cube JNU @Batul Pipewala @Lydia@Ishita and all others
[9:48 AM, 8/2/2021] Batul Pipewala: Please share pics of your Caenorhabditis nematodes isolated from jamun/badam trees
[9:50 AM, 8/2/2021] DP: Is there fruiting still seen anywhere, esp in the northernmost parts? @MANZOOR JAVAID in J&K or may be even in Delhi, Haryana Punjab areas?
[9:52 AM, 8/2/2021] Arunan: *commonly known as passion fruit, is a vine species of passion flower native to southern Brazil through Paraguay and northern Argentina.[1] It is cultivated commercially in tropical and subtropical areas for its sweet, seedy fruit.*Passiflora edulis - Wikipedia
[9:54 AM, 8/2/2021] Shraddha : No mam, there is only one set up.in 1st picture I was trying to show how plants kept inside & in 2nd picture position of box used to covered the plants.
[9:55 AM, 8/2/2021] Arunan : Comments please @Seethalakshmy CUBE @Shrudhiga and all Collaborators of Ant Model system!
You have made me fall for these!
Please join and let’s take it forward @Seethalakshmy CUBE
[9:58 AM, 8/2/2021] Arunan : So spiders are not insects? @Seema
Any reference?
[10:00 AM, 8/2/2021] Arunan: Amazing! @Seethalakshmy CUBE
Any reference you could find of these insects?
@Seethalakshmy CUBE
[10:02 AM, 8/2/2021] Arunan: Wonderful! @Sunita Chahar Same result means what? Please clarify.
[10:03 AM, 8/2/2021] Arunan : What do you mean by both sets? @Sunita Chahar
[10:03 AM, 8/2/2021] CUBist: Really amazing for doing this sir… What are this species called as sir? Do they have a local name?
[10:14 AM, 8/2/2021] CUBist: Plants kept in continous dark also did not close the leaves after 4 to 5 days
[10:25 AM, 8/2/2021] CUBist: According to the article we see fifteen stages of pupae sir…
1)stage 1:white puparium
2)stage 2: brown puparium
3)stage 3: bubble prepupa
4)stage 4:Bouyant
After the 4 rth stage we see in the article phenerocephalic pupa as a heading…we can stuck here what is phenerocephalic pupa?so we can read from 5 th stage to 13 stages so what we guess from here the pupa start to develop a bodypart …
5)stage 5:a)Malpighian tubules migrating
B)white Malpighian tubules
6)stage 6:green Malpighian tubules
7)stage 7: yellow body
8)stage 8: yellow eyed
9)stage 9:Amber
10)stage 10:red eye bald
11) stage 11: a)head bristle b) thoracic bristles
12)stage 12: a)wings tips grey b)wings grey
13) stage 13:wings black
14)stage 14 :mature bristles
15)stage 15:a) meconium b)eclosion
[10:28 AM, 8/2/2021] Arunan: What do we mean in Continuous Light?

What was the pattern of folding and unfolding of leaves, before keeping your Phyllanthus plant in Continuous light? @Sunita Chahar

Which species of Phyllanthus are you keeping in Normal light and in Continuous light?
Will be interesting get more clarity so that more cubists can join from thier HomeLabs!
[10:31 AM, 8/2/2021] Arunan: What is amazing about it? @Shrudhiga
I just kept a ruler with millimeter scale, as a reference to get the measurement of the ants!

Don’t you think that can tell us many things?
[10:31 AM, 8/2/2021] Arunan: What is amazing about it? @Shrudhiga
I just kept a ruler with millimeter scale, as a reference to get the measurement of the ants!

Don’t you think that can tell us many things? @Shrudhiga
[10:36 AM, 8/2/2021] CUBist: Yes sir… Size is also a criteria for the species identification of ants… There are also other criterias like color, body organization, and other special features of any one individual species
[10:38 AM, 8/2/2021] Arunan : *In my childhood, such ants were generally called “small black ants”! Sometimes, they were called ‘chonan’ in South Kerala. @Shrudhiga @Seethalakshmy CUBE

There used to be black chonans and yellow chonans!
However, in Mumbai, no one has names to call any ants to differentiate them! All are Ants!

They are all potential victims of insecticide spray, if they surpass their limit!
[10:39 AM, 8/2/2021] Arunan: Not clear @Sunita Chahar
[10:42 AM, 8/2/2021] Arunan: Please tell where you are stuck.
Other cubists will help! @Sidhy Kerala @Lakshmi PJ @Aswathy Suresh @Yash and others.
It will be a restart for them too in Cell phone microscopy @Aswathy Suresh @Janhvi Deshmukh @Aditya Joshi
[10:46 AM, 8/2/2021] Arunan HBSCE: The Phyllanthus in Continuous blight slept only for 3 to 4 hours? @P. Chitralekha
What about the Control plants, kept in normal sunlight light? @P. Chitralekha
@Lakshmi PJ what is your comment based on your experience?
[10:50 AM, 8/2/2021] Arunan : Won’t according to seasons, the length of day time change? More in summer months than in winter months, with Equinox in March & September having almost equal day and night length? @Sunita Chahar
[10:55 AM, 8/2/2021] Arunan : Ehy not we organize more collaborators to start looking at what happens to control plants, now on through Equinox times, 2nd & 3rd week of September, from across the country?
A COLLABORATIVE PROGRAM to celebrate 500 Days of CUBE HomeLab Movement! @P. Chitralekha @Sunita Chahar @Lydia @Batul Pipewala
[10:55 AM, 8/2/2021] Arunan : Why*
[11:02 AM, 8/2/2021] Arunan : Do you agree? @Janhvi Deshmukh and why?
[11:05 AM, 8/2/2021] CUBist: Yes sir I agree with chitra mam because there are three plant one plant are from different species one looks like phylluntus but leaf are so small and Also have thorns and we can’t se thorns in phyllunthus …and one is phyllunthus amarus sir …@Arunan
[11:06 AM, 8/2/2021] Arunan : Second time, this line is giving you trouble! @Aswathy Suresh
What do you think has happened?

Was there sometime you had more second generation flies in this bottle? @Aswathy Suresh
Don’t you have some other SLBK A bottles like SLBKA18, SLBKA19, with larvae, unhatched Pupae and adult flies? @Aswathy Suresh
[11:10 AM, 8/2/2021] KIRAN CUBE: Ohhh God😳 Why there’s some much of water condensation in this bottle?.
[11:11 AM, 8/2/2021] Arunan: What is your summary headline of all SLBK line fly stocks?

Please announce in one big headline! @Aswathy Suresh
[11:14 AM, 8/2/2021] Arunan : Why no news about SLBK C bottles? @Aswathy Suresh
Or, did I miss it because of too elaborate reporting style?
[11:18 AM, 8/2/2021] CUBist: Mushroom growth under our bathroom sink
[12:04 PM, 8/2/2021] Arunan E: Wow! @Ujaan Banerjee
Can you post another photo with better lighting?
Please give Location, Date and Name of Data Collector along with all photos.
Please post more!
[12:09 PM, 8/2/2021] Batul Pipewala: @Arunan@Anshu Kadam @Aswathy Suresh the nematodes are not liking the rava sugar vinegar medium
[12:10 PM, 8/2/2021] Arunan: How do we show the possibility of confusion between simple leaves of Phyllanthus and leaflets of some compound leaves, like Gulmohar tree?
@Janhvi Deshmukh @Sunita Chahar @P. Chitralekha @Yamal Gupta Cube KMC Delhi Botany Prof. @Shyam T.G
[12:15 PM, 8/2/2021] Arunan : Interesting! Why no water of condensation in SLBKB20 bottle in contrast to SLBK A21 bottle transferred the same day from the common stock medium?

@Aswathy Suresh Drosophila any urgent guess?@KIRAN @Yash @Lydia
[12:18 PM, 8/2/2021] Arunan: Here again, no water of condensation compared to SLBK A21 bottle of the same day! Let’s have some analysis! @Aswathy Suresh @Yash @Lydia
[12:21 PM, 8/2/2021] Arunan : Wow! @Aswathy Suresh
From just 2 flies transferred almost a month ago, so many pupal cases & so many flies! @Aswathy Suresh

How do you explain this? @Aswathy Suresh
[12:24 PM, 8/2/2021] Arunan : How about sprouting leaves? We can see some leaves on top with lighter colors! @Dr. Nisy Prasad Cube What do you think that is?
[12:26 PM, 8/2/2021] Arunan : What is meant as dormant state? What is its significance?
[12:28 PM, 8/2/2021] Arunan : What is your objective? And what is your conclusion? @Shraddha
[12:30 PM, 8/2/2021] Arunan : GRAT! @Janhvi Deshmukh Is this t just a chance happening or the bee is having some specific interaction like looking to flowers and feeding nectar? @Janhvi Deshmukh
[12:52 PM, 8/2/2021] Batul Pipewala: @Aswathy Suresh please collaborate as you too have microworms growing in rava medium
[1:37 PM, 8/2/2021] Anshu Kadam : Why?? @Batul Pipewala. Are you not able to see any nematodes?
How many hours since you transferred the nematodes to the medium?
Please share videos and photos of Rava Sugar Vinegar medium!!
[2:07 PM, 8/2/2021] CUBist: Open Bait setup janhvi deshmukh homelab bajaj collage of science wardha…
[2:07 PM, 8/2/2021] CUBist: It’s a second interlarva
[2:11 PM, 8/2/2021] Arunan: Please give essential details of the Dates when bait was started & Date of observation, Location & objective along with each photo @Janhvi Deshmukh
[2:11 PM, 8/2/2021] Arunan: Please give some reference link. @Janhvi Deshmukh
[2:19 PM, 8/2/2021] CUBist I was got 13,14 flies in my open bait setup on 29/07/2021 in my bait I used tomato and cucumber as a bait…at 8.30pm I got flies on it and closed it with the box tip …next day I observed 2 gravid fruitfly in the bait I kept this bait in my backyard near the dishwashing area…due moisture some flies are died …on 01/08/2021 I seen some larve on my bait …and in today observation I seen a second insterlarve and also some 1 insterlarve…
[2:20 PM, 8/2/2021] CUBist: This is 28/07/2021 when I trapped 13,14 flies …at 8.30 pm
[2:21 PM, 8/2/2021] CUBist: This video on 01/08/2021 when I see some larve moiving in bait…
[2:22 PM, 8/2/2021] CUBist: @Arunan @Aswathy Suresh @KIRAN CUBE @Batul Pipewala
[2:24 PM, 8/2/2021] Batul Pipewala: In how many days do you expect to see the pupae?
[2:46 PM, 8/2/2021] +91 03221: Two day mam @Batul Pipewala
[2:50 PM, 8/2/2021] Arunan: A slice of the 1st day of CUBE National Meet 19, 20, 21March 2021 5-30pm to 9-30 pm https://webinar.hbcse.tifr.res.in/b/kir-vgc-6jf

Meta Studio

[2:53 PM, 8/2/2021] Arunan : Why? @Janhvi Deshmukh What is the reasoning?
[3:44 PM, 8/2/2021] Yash :
[3:45 PM, 8/2/2021] Yash: @Batul Pipewala there is another nematode that is moving here… Near the soil… @Anshu Kadam @Arunan@Shivam CUBE
[3:46 PM, 8/2/2021] Yash: Wow @Janhvi Deshmukh
Can you share the reference to this Article…
Which pupae are you able to See in the Bait that you have?
@Aswathy Suresh @Arunan@Lydia
[3:48 PM, 8/2/2021] Yash : Lets hope for the gravid looking like fruit fly in the fresh TRS-V media
To lay eggs @Aswathy Suresh sN nattika Drosophila
[3:49 PM, 8/2/2021] Yash : @Aswathy Suresh
[3:50 PM, 8/2/2021] KIRAN CUBE: 500th Day of CUBE chatShaala: 01st August 2021
43 CUBists had joined the webinar
INDIA

Assam
Jorhat: Ishita Sonowal

Delhi
New Delhi: Raj Yashwantrao Patil

Maharashtra
Bandra: Saida Sayyed
Anchal Yadav
Manasi Parab
Kinjal Gohli
Vinay Gahlot
Gautam Maurya
Karan Fulwariya
Divya Rao
Jasmin Shaikh
Sachi Bind
MC Arunan
Chaitanya Gaikwad
Raj Ptail
Sunita Chahar
Sarita Kumar
Anjani Kumar Kashyap
Sachin Pradhan
Anshu Kadam
Rachana Acharya
Shalini Gautam
Lydia Mathew
Shraddha sonavane
Aditya Joshi
Khan shahreen
Janhvi Deshmukh
Priti Kanade
Kareena Dhuri

Jharkhand
Man Masih Beck

Kerala
Aswathy Suresh
Vishnupriya
Seethalakshmi
Lakshmy
Arunima
Shyam TG

Kolenchery
Abhijith Vinod
Ajin

Uttar Pradesh
Moradabad: Kiran Yadav

Uttrakhand
Darshana V. …
[3:51 PM, 8/2/2021] Yash: Breaking News Update
More fruit flies observed in Fruit fly cultures at 9th day from Transferring a Single Gravid fruit fly!

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from Locality

Location - Prabhadevi, Mumbai
Gravid fruit fly Transferred on - 24/07/2021

3 day observation
SLPbA1
Breaking News - ~ 15 First generation Fruit flies arrive at the 7th Day of Transfer ring Gravid Fruit fly
Date and time of video - 31/07/2021 - 11:45 AM

31/07/2021
Observation
No of Fruit flies - ~ 15
No of pupae - ~ 70
No of larvae - - 12 - 3mm larvae, 5 - 2mm

1/08/2021
Observation
No of fruit flies - ~40
No of unhatched pupae - 40

2/08/2021
Observation
No of fruit flies - ~50
No do unh…
[3:53 PM, 8/2/2021] CUBist: Actually I didn’t see any pupae i see only the larve @Yash Isc Xmas Cube
[3:54 PM, 8/2/2021] CUBist: @Yash Isc Xmas Cube
[3:59 PM, 8/2/2021] Yash I: Location - Prabhadevi, Mumbai
Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from Locality

Gravid fruit fly Transferred on - 24/07/2021
SLPbB1

31/07/2021
Observation
No of Fruit flies - 8
No of pupae - 56
No of larvae - 3- 3mm Larvae, 12 - 2mm larvae

1/08/2021
No of fruit flies - ~ 25
No of unhatched pupae - 35
No of larvae - 8 - 3mm larvae

2/08/2021
No of fruit flies - ~ 30
No of unhatched pupae - ~30
No of larvae - 12 - 3mm larana
alert - No of dead flies - 8 fruit flies
[4:07 PM, 8/2/2021] Yash: Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from Locality

Update of Single species culture of Fruit flies
Location - Prabhadevi, Mumbai
SLPbC1

31/07/2021
Observation
No of Fruit flies - 5
No of pupae - ~ 60
No of larvae - 10- 3mm larvae

1/08/2021
Observation
No of fruit flies - 20
No of unhatched pupae - 32
No of larvae - 7 - 3mm larvae

2/08/2021
Observation
No of fruit flies - ~35
No of unhatched pupae - ~ 40
No of larvae - 8 - 3mm larvae
[4:10 PM, 8/2/2021] Dr. Sarita Kumar : Hi Kiran…my name has been entered under Maharashtra😊
[4:11 PM, 8/2/2021] KIRAN CUBE: Thanks for the correction.
We will do the needful
[4:13 PM, 8/2/2021] Yash: Guess? guess!!.. Different Stages of Pupae seen in SLPbC Culture! -
Date - 2/08/2021.
Location - Prabhadevi, Mumbai
I am guessing the one marked in Orange marked is an empty pupal case.
Blue marked - Yellow Pupae
green marked - Wing stage Pupae?!
@Janhvi Deshmukh @Arunan HBSCE sir @KIRAN CUBE maam @Batul Pipewala Kolkata maam @Aswathy Suresh sN nattika Drosophila @Lydia cUBe Drosphila @Isha Pawle @Aastha Ahuja
[4:16 PM, 8/2/2021] KIRAN CUBE: Woow am i able to see the red color of eye in the right bottom pupa marked under green circle.
[4:34 PM, 8/2/2021] Dr. Sarita:
[4:58 PM, 8/2/2021] Seethalakshmy CUBE: Great @Yash
What is the black dot present it?
Empty pupal case as well as yellow pupal case can able see this black dot.
Green marked one it’s not clearly visible, so i don’t know is present or not.
[5:00 PM, 8/2/2021] Aastha : I think that black dot is the mouth part of the larvae in blue and orange circle
[5:00 PM, 8/2/2021] Seethalakshmy CUBE: What is wing stage pupae?
Can you explain?
[5:01 PM, 8/2/2021] Aastha : Why does the color of early pupa in blue circle to the late pupa in orange circle change to dark brown from whitish ?
[5:02 PM, 8/2/2021] Aastha: Green circle pupa has wings in it they are the black color lines present on both sides of pupa
[5:05 PM, 8/2/2021] Aastha: Arrow indicates the black line in the pupa which is wing @Seethalakshmy CUBE , photo credits @Yash
[5:21 PM, 8/2/2021]CUBist: I think the yellow round is stage 7 : yellow body
[5:25 PM, 8/2/2021] Seethalakshmy CUBE: where did you find these ant? @Arunan HBSCE sir
Size of the ant is 2 mm right?
Is it lonely? Have you observed what it does?
[5:29 PM, 8/2/2021] CUBist: And green circle is a stage 12 :wings tips grey I guess
@Yash
[5:47 PM, 8/2/2021] Aastha : Can you please resend the reference for stages of pupa I think it was shared earlier but my bad I missed it
[5:57 PM, 8/2/2021] Seethalakshmy CUBE: I think this is a worker ant.
Beause according to it’s size as well as it’s behavior
Only the worker ant searching food

[KiranKalakotiR (Kiran Kalakoti)]

1st August 9.30pm to 2nd August 9.30pm

[9:42 PM, 8/1/2021] Dr. Nisy Prasad: Mango Tree 1 at Muthalamada, Palakkad, Kerala,10.6037° N, 76.7365° E… Tree don’t have Fruits,Flowers,Flushing Leaves… Date: 1.8.2021,… Nisy Prasad,

[9:43 PM, 8/1/2021] Dr. Nisy Prasad: Mango Tree 2 at Muthalamada, Palakkad, Kerala,10.6037° N, 76.7365° E… Tree Don’t Have Flushing Leaves, Flowers, Fruits… Date: 1.8.2021,…Nisy Prasad

[9:43 PM, 8/1/2021] Dr. Nisy Prasad: Mango Trees3 & 4 at Muthalamada , Palakkad, Kerala…10.6037° N, 76.7365° E… Trees don’t have Flushing Leaves, Flowers,Fruits… Date : 1.8.2021,…Nisy Prasad

[9:44 PM, 8/1/2021] Dr. Nisy Prasad: Mango Tree 5 at Muthalamada, Palakkad,Kerala… 10.6037° N, 76.7365° E… Tree don’t have Flushing Leaves, Flowers, Fruits… Date: 1.8.2021… by Nisy Prasad

[9:44 PM, 8/1/2021] Dr. Nisy Prasad: Summary…

Total Trees Surveyed = 16.

Trees with out Flushing Leaves, Flowers, Fruits = 16

All the Trees Found to be at Dormant state… Place : Muthalamada, Palakkad, Kerala

Latitude & Longitude : 10.6037° N, 76.7365° E

Date : 1.8.2021, Time : 2.30pm.

Data by Nisy Prasad, CUBE, Palakkad.

[10:33 PM, 8/1/2021] Shraddha: Continuous light experiment-

day 1(1/8/21)

plants transferred under continuous light 31/07/21(8:30pm)

leaf closing time-

1- 8:55pm

2- 9:02pm

3-9:05pm

4-8:50 pm

intensity of light- 472-533Lux

location- Panvel.

data collected by- Shraddha.

[10:33 PM, 8/1/2021] Shraddha CUBE: Control set up-

day 1(1/8/21)

leaf closing time-

1- 6:35pm

2- 6:25pm

3-6:40pm

4-6:59 pm

sunset time- 7:13pm

intensity of light- 558Lux

location- Panvel.

data collected by- Shraddha.

[10:33 PM, 8/1/2021] Shraddha CUBE: Continuous dark experiment-

day 1(1/8/21)

plants transferred under continuous dark 31/07/21(8:30pm)

leaf closing time-

1- 8:10pm

2- 8:30pm

3-8:25pm

4-6:20 pm

intensity of light- 0 Lux

location- Panvel.

data collected by- Shraddha.

[10:43 PM, 8/1/2021] Sunita Chahar: Shraddha, very good set up

[10:43 PM, 8/1/2021] Sunita Chahar: I will suggest to take photos through timestamp app

[11:48 PM, 8/1/2021] +91-03221: I see honeybee in my phylluntus …

[11:50 PM, 8/1/2021] Man Masih Beck: *Summary of 500th Day of CUBE ChatShaala:

1st Aug, 2021*

The time I joined, discussion was on- Are Moinas attracted to light?

Many claims of observing Moina being attracted to light have been made by the CUBists since long. To end the debate, @Saida Cube Rotifers and her brother are trying to design an experiment to unveil the truth.

A Cuboidal fish tank filled with DC water is taken. The tank is divided into hypothetical compartments of A to E Columns and 1 to 3 Rows, coordinates of which are to be known from A1 to E3.

When torched from E2, the Moinas in the tank started accumulating at D2 which indicates that Moinas could be attracted towards light.

While discussing the color change observed in the Moinas, @Sunita Chahar prompted an important discussion if M…

[11:54 PM, 8/1/2021] Man Masih Beck: Screenshots: 1st Aug, 2021

[12:41 AM, 8/2/2021] Shraddha CUBE: Mam actually, I am combining 4 plants data so, I could not used timestamp.timestamp shows only time when I will captured photo.

[12:49 AM, 8/2/2021] +91 86570 87106: They shows location too.

[12:51 AM, 8/2/2021] Shraddha CUBE: Ohh!!

Ok I will try to capture with timestamp app

[12:58 AM, 8/2/2021] Shraddha CUBE: Temperature-27°C (01/08/21)

Vihighar, Maharashtra, India Daily Weather | AccuWeather

[1:09 AM, 8/2/2021] Jai Kishan CUBE: Why don’t we find out the role of Anthocyanin pigments

[7:22 AM, 8/2/2021] Batul Pipewala Kolkata: Great set up with replicates and controls .

What is the time the leaves opened next day? Dont you think that is also important?

[7:49 AM, 8/2/2021] Batul Pipewala Kolkata: Nematode moving on rava sugar vinegar medium 10minutes after transfer 1/8/21 Homelab Kolkata Batul

[7:51 AM, 8/2/2021] Batul Pipewala Kolkata: No movement of nematodes on the rava sugar vinegar medium 24hrs after transfer, the whole plate has been scanned have they gone deep in the rava or are they dead?

[8:27 AM, 8/2/2021] P. Chitralekha: Yes. Great work Shraddha. How did you provide continuous dark condition?

[9:08 AM, 8/2/2021] Lydia: As a testable hypothesis, we can state that if adult flies emerge from the trapped pupae kept inside the bottle, then this means that there is no harm in detaching the pupa from its surface onto which it sticks during pupation or that it won’t harm the pupa in the process of development, and hence we can observe its further developmental stages.

[9:17 AM, 8/2/2021] Lydia: There is a lot of condensation in this bottle @Aswathy Suresh CUBE

What do you do immediately after you pour the medium into the bottle?

Do you allow the media bottle to cool down?

[9:20 AM, 8/2/2021] Arunan HBSCE: What are the fifteen stages of pupae, according to the article? @Janhvi Deshmukh

[9:21 AM, 8/2/2021] Arunan HBSCE: Daily update please! Expectation, Observation along with interpretation, please!

[9:33 AM, 8/2/2021] Shraddha CUBE: Thank you😊 @Sunita Chahar ma’am @Batul Pipewala Kolkata ma’am @P. Chitralekha mam

[9:33 AM, 8/2/2021] Shraddha CUBE: Yes @Batul Pipewala Kolkata mam leaves opened as well as closed time is most important. I think on basis of that only we can conclude whether 24hrs continuous light & dark exposure affected or not.

[9:34 AM, 8/2/2021] Arunan HBSCE: @KIRAN CUBE

[9:34 AM, 8/2/2021] Shraddha CUBE: I Kept plants in box like place under cupboard & this covered by another box by placing half inside & half outside.

So it’s helping me to maintain continuous dark conditions & in aeration also.

[9:36 AM, 8/2/2021] Arunan HBSCE: Sorry, sent as an appetizer for Kitchen Lab Movement!

Looking for the real ones, from others!

[9:37 AM, 8/2/2021] Arunan HBSCE: Any news on Pagalapos studies? @Lydia

@Hina Mudgal@Lakshmi PJ@KIRAN CUBE @P. Chitralekha @Aditya Joshi

[9:38 AM, 8/2/2021] Arunan HBSCE: Looking for Monsoon Fast Plants @Shraddha CUBE @Yash Sheregare@Lakshmi PJ@KIRAN CUBE and others.

[9:42 AM, 8/2/2021] Arunan HBSCE: @Lydia Please give a design stepwise, so that all of us can test your Hypothesis, in our HomeLabs

[9:44 AM, 8/2/2021] Arunan HBSCE: Good question @Lydia

Can @Aswathy Suresh CUBE @Aastha Ahuja Cube @Janhvi Deshmukh comment?

What is the comment by @Lydia ?

[9:46 AM, 8/2/2021] Batul Pipewala Kolkata: Are there two set ups 1 and 2?How are they different

[9:46 AM, 8/2/2021] P. Chitralekha:

[9:46 AM, 8/2/2021] Anshu Kadam@Arunan HBSCE sir I’m not sure in which soil sample we will find C. elegans. But according to our previous findings and observations, we found Caenorhabditis genus nematodes in soil sample of Jamun tree of Ratnam College, Bhandup and Almond tree in HBCSE surroundings.

@Batul Pipewala Kolkata ma’am which soil sample are you using? And if possible for you, then try getting soil sample from some fruiting trees!!!

[9:47 AM, 8/2/2021] Arunan HBSCE: Any one has Mango trees in their locality in sprouting, flowering or fruiting in the First week of August?

@Lakshmi PJ@Dr. Nisy Prasad Cube @Shrudhiga @KIRAN CUBE @P. Chitralekha @Batul Pipewala Kolkata @Lydia @Ishita Assamand all others

[9:48 AM, 8/2/2021] Batul Pipewala Kolkata: Please share pics of your Caenorhabditis nematodes isolated from jamun/badam trees

[9:50 AM, 8/2/2021] DP HBCSE: Is there fruiting still seen anywhere, esp in the northernmost parts? @MANZOOR JAVAID in J&K or may be even in Delhi, Haryana Punjab areas?

[9:52 AM, 8/2/2021] Arunan HBSCE: *commonly known as passion fruit, is a vine species of passion flower native to southern Brazil through Paraguay and northern Argentina.[1] It is cultivated commercially in tropical and subtropical areas for its sweet, seedy fruit.*Passiflora edulis - Wikipedia

[9:54 AM, 8/2/2021] Shraddha CUBE: No mam, there is only one set up.in 1st picture I was trying to show how plants kept inside & in 2nd picture position of box used to covered the plants.

[9:55 AM, 8/2/2021] Arunan HBSCE: Comments please @Seethalakshmy CUBE @Shrudhiga and all Collaborators of Ant Model system!

You have made me fall for these!

Please join and let’s take it forward @Seethalakshmy CUBE

[9:58 AM, 8/2/2021] Arunan HBSCE: So spiders are not insects? @Seema

Any reference?

[10:00 AM, 8/2/2021] Arunan HBSCE: Amazing! @Seethalakshmy CUBE

Any reference you could find of these insects?

@Seethalakshmy CUBE

[10:02 AM, 8/2/2021] Arunan HBSCE: Wonderful! @Sunita Chahar Same result means what? Please clarify.

[10:03 AM, 8/2/2021] Arunan HBSCE: What do you mean by both sets? @Sunita Chahar

[10:03 AM, 8/2/2021] Shrudiga: Really amazing for doing this sir… What are this species called as sir? Do they have a local name?

[10:14 AM, 8/2/2021] Sunita Chahar: Plants kept in continous dark also did not close the leaves after 4 to 5 days

[10:25 AM, 8/2/2021] Janhvi: According to the article we see fifteen stages of pupae sir…

1)stage 1:white puparium

2)stage 2: brown puparium

3)stage 3: bubble prepupa

4)stage 4:Bouyant

After the 4 rth stage we see in the article phenerocephalic pupa as a heading…we can stuck here what is phenerocephalic pupa?so we can read from 5 th stage to 13 stages so what we guess from here the pupa start to develop a bodypart …

5)stage 5:a)Malpighian tubules migrating

B)white Malpighian tubules

6)stage 6:green Malpighian tubules

7)stage 7: yellow body

8)stage 8: yellow eyed

9)stage 9:Amber

10)stage 10:red eye bald

11. stage 11: a)head bristle b) thoracic bristles

12)stage 12: a)wings tips grey b)wings grey

13. stage 13:wings black

14)stage 14 :mature bristles

15)stage 15:a) meconium b)eclosion

[10:28 AM, 8/2/2021] Arunan HBSCE: What do we mean in Continuous Light?

What was the pattern of folding and unfolding of leaves, before keeping your Phyllanthus plant in Continuous light? @Sunita Chahar

Which species of Phyllanthus are you keeping in Normal light and in Continuous light?

Will be interesting get more clarity so that more cubists can join from thier HomeLabs!

[10:31 AM, 8/2/2021] Arunan HBSCE: What is amazing about it? @Shrudhiga

I just kept a ruler with millimeter scale, as a reference to get the measurement of the ants!

Don’t you think that can tell us many things?

[10:31 AM, 8/2/2021] Arunan HBSCE: What is amazing about it? @Shrudhiga

I just kept a ruler with millimeter scale, as a reference to get the measurement of the ants!

Don’t you think that can tell us many things? @Shrudhiga

[10:36 AM, 8/2/2021] Shrudiga: Yes sir… Size is also a criteria for the species identification of ants… There are also other criterias like color, body organization, and other special features of any one individual species

[10:38 AM, 8/2/2021] Arunan HBSCE: *In my childhood, such ants were generally called “small black ants”! Sometimes, they were called ‘chonan’ in South Kerala. @Shrudhiga @Seethalakshmy CUBE

There used to be black chonans and yellow chonans!

However, in Mumbai, no one has names to call any ants to differentiate them! All are Ants!

They are all potential victims of insecticide spray, if they surpass their limit!

[10:39 AM, 8/2/2021] Arunan HBSCE: Not clear @Sunita Chahar

[10:42 AM, 8/2/2021] Arunan HBSCE: Please tell where you are stuck.

Other cubists will help! @Sidhy Kerala Drosophila @Lakshmi PJ@Aswathy Suresh CUBE @Yash Sheregareand others.

It will be a restart for them too in Cell phone microscopy @Aswathy Suresh CUBE @Janhvi Deshmukh @Aditya Joshi

[10:46 AM, 8/2/2021] Arunan HBSCE: The Phyllanthus in Continuous blight slept only for 3 to 4 hours? @P. Chitralekha

What about the Control plants, kept in normal sunlight light? @P. Chitralekha

@Lakshmi PJwhat is your comment based on your experience?

[10:50 AM, 8/2/2021] Arunan HBSCE: Won’t according to seasons, the length of day time change? More in summer months than in winter months, with Equinox in March & September having almost equal day and night length? @Sunita Chahar

[10:55 AM, 8/2/2021] Arunan HBSCE: Ehy not we organize more collaborators to start looking at what happens to control plants, now on through Equinox times, 2nd & 3rd week of September, from across the country?

A COLLABORATIVE PROGRAM to celebrate 500 Days of CUBE HomeLab Movement! @P. Chitralekha @Sunita Chahar @Lydia @Batul Pipewala Kolkata

[10:55 AM, 8/2/2021] Arunan HBSCE: Why*

[11:02 AM, 8/2/2021] Arunan HBSCE: Do you agree? @Janhvi Deshmukh and why?

[11:05 AM, 8/2/2021] Janhvi: Yes sir I agree with chitra mam because there are three plant one plant are from different species one looks like phylluntus but leaf are so small and Also have thorns and we can’t se thorns in phylluntus …and one is phylluntus amarus sir …@Arunan HBSCE sir

[11:06 AM, 8/2/2021] Arunan HBSCE: Second time, this line is giving you trouble! @Aswathy Suresh CUBE

What do you think has happened?

Was there sometime you had more second generation flies in this bottle? @Aswathy Suresh CUBE

Don’t you have some other SLBK A bottles like SLBKA18, SLBKA19, with larvae, unhatched Pupae and adult flies? @Aswathy Suresh CUBE

[11:10 AM, 8/2/2021] KIRAN CUBE: Ohhh God😳 Why there’s some much of water condensation in this bottle?.

[11:11 AM, 8/2/2021] Arunan HBSCE: What is your summary headline of all SLBK line fly stocks?

Please announce in one big headline! @Aswathy Suresh CUBE

[11:14 AM, 8/2/2021] Arunan HBSCE: Why no news about SLBK C bottles? @Aswathy Suresh CUBE

Or, did I miss it because of too elaborate reporting style?

[11:18 AM, 8/2/2021] +91 14022: Mushroom growth under our bathroom sink

[12:04 PM, 8/2/2021] Arunan HBSCE: Wow! @Ujaan Banerjee

Can you post another photo with better lighting?

Please give Location, Date and Name of Data Collector along with all photos.

Please post more!

[12:09 PM, 8/2/2021] Batul Pipewala Kolkata: @Arunan HBSCE @Anshu Kadam (Nematodes) @Aswathy Suresh CUBE the nematodes are not liking the rava sugar vinegar medium

[12:10 PM, 8/2/2021] Arunan HBSCE: How do we show the possibility of confusion between simple leaves of Phyllanthus and leaflets of some compound leaves, like Gulmohar tree?

@Janhvi Deshmukh @Sunita Chahar @P. Chitralekha @Yamal Gupta Cube KMC Delhi Botany Prof. @Shyam T.G Cube SN Nattika Kerala

[12:15 PM, 8/2/2021] Arunan HBSCE: Interesting! Why no water of condensation in SLBKB20 bottle in contrast to SLBK A21 bottle transferred the same day from the common stock medium?

@Aswathy Suresh CUBE any urgent guess?@KIRAN CUBE @Yash Sheregare@Lydia

[12:18 PM, 8/2/2021] Arunan HBSCE: Here again, no water of condensation compared to SLBK A21 bottle of the same day! Let’s have some analysis! @Aswathy Suresh CUBE @Yash Sheregare@Lydia

[12:21 PM, 8/2/2021] Arunan HBSCE: Wow! @Aswathy Suresh CUBE

From just 2 flies transferred almost a month ago, so many pupal cases & so many flies! @Aswathy Suresh CUBE

How do you explain this? @Aswathy Suresh CUBE

[12:24 PM, 8/2/2021] Arunan HBSCE: How about sprouting leaves? We can see some leaves on top with lighter colors! @Dr. Nisy Prasad Cube What do you think that is?

[12:26 PM, 8/2/2021] Arunan HBSCE: What is meant as dormant state? What is its significance?

[12:28 PM, 8/2/2021] Arunan HBSCE: What is your objective? And what is your conclusion? @Shraddha CUBE

[12:31 PM, 8/2/2021] Arunan HBSCE: GRAT! @Janhvi Deshmukh Is this t just a chance happening or the bee is having some specific interaction like looking to flowers and feeding nectar? @Janhvi Deshmukh

[12:52 PM, 8/2/2021] Batul Pipewala Kolkata: @Aswathy Suresh CUBE please collaborate as you too have microworms growing in rava medium

[1:37 PM, 8/2/2021] Anshu KadamWhy?? @Batul Pipewala Kolkata ma’am. Are you not able to see any nematodes?

How many hours since you transferred the nematodes to the medium?

Please share videos and photos of Rava Sugar Vinegar medium!!

[2:07 PM, 8/2/2021] Janhvi: Open Bait setup janhvi deshmukh homelab bajaj collage of science wardha…

[2:07 PM, 8/2/2021] Janhvi: It’s a second interlarva

[2:11 PM, 8/2/2021] Arunan HBSCE: Please give essential details of the Dates when bait was started & Date of observation, Location & objective along with each photo @Janhvi Deshmukh

[2:12 PM, 8/2/2021] Arunan HBSCE: Please give some reference link. @Janhvi Deshmukh

[2:19 PM, 8/2/2021] Janhvi: I was got 13,14 flies in my open bait setup on 29/07/2021 in my bait I used tomato and cucumber as a bait…at 8.30pm I got flies on it and closed it with the box tip …next day I observed 2 gravid fruitfly in the bait I kept this bait in my backyard near the dishwashing area…due moisture some flies are died …on 01/08/2021 I seen some larve on my bait …and in today observation I seen a second insterlarve and also some 1 insterlarve…

[2:20 PM, 8/2/2021] Janhvi: This is 28/07/2021 when I trapped 13,14 flies …at 8.30 pm

[2:21 PM, 8/2/2021] Janhvi: This video on 01/08/2021 when I see some larve moiving in bait…

[2:22 PM, 8/2/2021] Janhvi: @Arunan HBSCE sir @Aswathy Suresh CUBE @KIRAN CUBE @Batul Pipewala Kolkata mam

[2:24 PM, 8/2/2021] Batul Pipewala Kolkata: In how many days do you expect to see the pupae?

[2:46 PM, 8/2/2021] Janhvi: Two day mam @Batul Pipewala Kolkata mam

[2:50 PM, 8/2/2021] Arunan HBSCE: A slice of the 1st day of CUBE National Meet 19, 20, 21March 2021 5-30pm to 9-30 pm https://webinar.hbcse.tifr.res.in/b/kir-vgc-6jf

Meta Studio

[2:53 PM, 8/2/2021] Arunan HBSCE: Why? @Janhvi Deshmukh What is the reasoning?

[3:44 PM, 8/2/2021] Yash Sheregare:

[3:45 PM, 8/2/2021] Yash Sheregare: @Batul Pipewala Kolkata maam there is another nematode that is moving here… Near the soil… @Anshu Kadam (Nematodes) @Arunan HBSCE sir @Shivam CUBE NEMATODE

[3:46 PM, 8/2/2021] Yash Sheregare: Wow @Janhvi Deshmukh

Can you share the reference to this Article…

Which pupae are you able to See in the Bait that you have?

@Aswathy Suresh CUBE @Arunan HBSCE sir @Lydia

[3:48 PM, 8/2/2021] Yash Sheregare: Lets hope for the gravid looking like fruit fly in the fresh TRS-V media

To lay eggs @Aswathy Suresh CUBE

[3:49 PM, 8/2/2021] Yash Sheregare: @Aswathy Suresh CUBE

[3:50 PM, 8/2/2021] KIRAN CUBE: 500th Day of CUBE chatShaala: 01st August 2021

43 CUBists had joined the webinar

INDIA

Assam

Jorhat: Ishita Sonowal

Delhi

New Delhi: Raj Yashwantrao Patil

Maharashtra

Bandra: Saida Sayyed

Anchal Yadav

Manasi Parab

Kinjal Gohli

Vinay Gahlot

Gautam Maurya

Karan Fulwariya

Divya Rao

Jasmin Shaikh

Sachi Bind

MC Arunan

Chaitanya Gaikwad

Raj Ptail

Sunita Chahar

Sarita Kumar

Anjani Kumar Kashyap

Sachin Pradhan

Anshu Kadam

Rachana Acharya

Shalini Gautam

Lydia Mathew

Shraddha sonavane

Aditya Joshi

Khan shahreen

Janhvi Deshmukh

Priti Kanade

Kareena Dhuri

Jharkhand

Man Masih Beck

Kerala

Aswathy Suresh

Vishnupriya

Seethalakshmi

Lakshmy

Arunima

Shyam TG

Kolenchery

Abhijith Vinod

Ajin

Uttar Pradesh

Moradabad: Kiran Yadav

Uttrakhand

Darshana V. …

[3:51 PM, 8/2/2021] Yash Sheregare: Breaking News Update

More fruit flies observed in Fruit fly cultures at 9th day from Transferring a Single Gravid fruit fly!

Short term Objective - 1) Culturing Single species Native fruit flies

2. Identify and culture Drosophila melanogaster species from Locality

Location - Prabhadevi, Mumbai

Gravid fruit fly Transferred on - 24/07/2021

3 day observation

SLPbA1

Breaking News - ~ 15 First generation Fruit flies arrive at the 7th Day of Transfer ring Gravid Fruit fly

Date and time of video - 31/07/2021 - 11:45 AM

31/07/2021

Observation

No of Fruit flies - ~ 15

No of pupae - ~ 70

No of larvae - - 12 - 3mm larvae, 5 - 2mm

1/08/2021

Observation

No of fruit flies - ~40

No of unhatched pupae - 40

2/08/2021

Observation

No of fruit flies - ~50

No do unh…

[3:53 PM, 8/2/2021] Janhvi: Actually I didn’t see any pupae i see only the larve @Yash Sheregare

[3:54 PM, 8/2/2021] Janhvi: @Yash Sheregare

[3:59 PM, 8/2/2021] Yash Sheregare: Location - Prabhadevi, Mumbai

Short term Objective - 1) Culturing Single species Native fruit flies

2. Identify and culture Drosophila melanogaster species from Locality

Gravid fruit fly Transferred on - 24/07/2021

SLPbB1

31/07/2021

Observation

No of Fruit flies - 8

No of pupae - 56

No of larvae - 3- 3mm Larvae, 12 - 2mm larvae

1/08/2021

No of fruit flies - ~ 25

No of unhatched pupae - 35

No of larvae - 8 - 3mm larvae

2/08/2021

No of fruit flies - ~ 30

No of unhatched pupae - ~30

No of larvae - 12 - 3mm larana

alert - No of dead flies - 8 fruit flies

[4:07 PM, 8/2/2021] Yash Sheregare: Short term Objective - 1) Culturing Single species Native fruit flies

2. Identify and culture Drosophila melanogaster species from Locality

Update of Single species culture of Fruit flies

Location - Prabhadevi, Mumbai

SLPbC1

31/07/2021

Observation

No of Fruit flies - 5

No of pupae - ~ 60

No of larvae - 10- 3mm larvae

1/08/2021

Observation

No of fruit flies - 20

No of unhatched pupae - 32

No of larvae - 7 - 3mm larvae

2/08/2021

Observation

No of fruit flies - ~35

No of unhatched pupae - ~ 40

No of larvae - 8 - 3mm larvae

[4:10 PM, 8/2/2021] Dr. Sarita kumar Delhi CUBE: Hi Kiran…my name has been entered under Maharashtra😊

[4:11 PM, 8/2/2021] KIRAN CUBE: Thanks for the correction.

We will do the needful

[4:13 PM, 8/2/2021] Yash Sheregare: Guess? guess!!.. Different Stages of Pupae seen in SLPbC Culture! -

Date - 2/08/2021.

Location - Prabhadevi, Mumbai

I am guessing the one marked in Orange marked is an empty pupal case.

Blue marked - Yellow Pupae

green marked - Wing stage Pupae?!

@Janhvi Deshmukh @Arunan HBSCE sir @KIRAN CUBE maam @Batul Pipewala Kolkata maam @Aswathy Suresh CUBE @Lydia @Isha Pawle @Aastha Ahuja Cube

[4:16 PM, 8/2/2021] KIRAN CUBE: Woow am i able to see the red color of eye in the right bottom pupa marked under green circle.

[4:34 PM, 8/2/2021] Dr. Sarita kumar Delhi CUBE:

[4:58 PM, 8/2/2021] Seethalakshmy CUBE: Great @Yash Sheregare​:+1:

What is the black dot present it?

Empty pupal case as well as yellow pupal case can able see this black dot.

Green marked one it’s not clearly visible, so i don’t know is present or not.

[5:00 PM, 8/2/2021] Aastha Ahuja Cube: I think that black dot is the mouth part of the larvae in blue and orange circle

[5:00 PM, 8/2/2021] Seethalakshmy CUBE: What is wing stage pupae?

Can you explain?

[5:01 PM, 8/2/2021] Aastha Ahuja Cube: Why does the color of early pupa in blue circle to the late pupa in orange circle change to dark brown from whitish ?

[5:02 PM, 8/2/2021] Aastha Ahuja Cube: Green circle pupa has wings in it they are the black color lines present on both sides of pupa

[5:05 PM, 8/2/2021] Aastha Ahuja Cube: Arrow indicates the black line in the pupa which is wing @Seethalakshmy CUBE , photo credits @Yash Sheregare

[5:21 PM, 8/2/2021] Janhvi: I think the yellow round is stage 7 : yellow body

[5:25 PM, 8/2/2021] Seethalakshmy CUBE: where did you find these ant? @Arunan HBSCE sir

Size of the ant is 2 mm right?

Is it lonely? Have you observed what it does?

[5:29 PM, 8/2/2021] Janhvi: And green circle is a stage 12 :wings tips grey I guess

@Yash Sheregare

[5:47 PM, 8/2/2021] Aastha Ahuja Cube: Can you please resend the reference for stages of pupa I think it was shared earlier but my bad I missed it

[5:57 PM, 8/2/2021] Seethalakshmy CUBE: I think this is a worker ant.

Beause according to it’s size as well as it’s behavior

Only the worker ant searching food

[6:05 PM, 8/2/2021] Seethalakshmy CUBE: https://ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=8537

Spiders, as the Explorit Science Center explains, have two main body parts. “The body consists of a combined head and thorax called the cephalothorax, and the abdomen. The cephalothorax has the eyes, mouthparts (no antennae) and four pairs of legs. Animals that share these characteristics include ticks, mites, scorpions and spiders. The group is called the Arachnida.”

[6:33 PM, 8/2/2021] Janhvi: @Aastha Ahuja Cube

[6:36 PM, 8/2/2021] Seethalakshmy CUBE: Cuckoo’ s voice

Location : Pazhuvil, Thrissur

Date : 2/8/21

Time : 6:32 pm

Data collector : Seethalakshmi. C. R. Cube Nattika

[6:36 PM, 8/2/2021] Janhvi: Yes😌

[6:43 PM, 8/2/2021] Arunan HBSCE: Potential Mosquito breeding sites, 30 July 2021 Bhulabhai Desai Road, South Mumbai Photo: mcarunan CUBE Sophia HomeLab, South Mumbai

[7:01 PM, 8/2/2021] Gautam J Mourya Cube: https://www.vedicus.com/patharchatta

I have this plant at my home

This plant is effective for the ones who has kidney stones

[7:04 PM, 8/2/2021] Gautam J Mourya Cube: It’s scientific name is BRYOPHYLLUM PINNATUM

[7:16 PM, 8/2/2021] Batul Pipewala Kolkata: pic@@Yash Sheregare

[7:18 PM, 8/2/2021] Arunan HBSCE: Please join in today’s Chatshaala if not joined yet. Interesting discussion going on.

[7:50 PM, 8/2/2021] Aastha Ahuja Cube: thanks

[8:08 PM, 8/2/2021] KIRAN CUBE: Interesting discussion is going on "How Galapagos and Pagalapos islands are simmilar?

Kindly join the CUBE chatShaala @Batul Pipewala Kolkata @Saida Cube Rotifers @Isha Pawle @Ishita Assam @Raj Elphinstone Cube @Aswathy Suresh CUBE @Lydia and other Cubists

[9:09 PM, 8/2/2021] Shraddha CUBE: Continuous dark experiment-

day 2 (2/8/21)

plants transferred under continuous dark 31/07/21(8:30pm)

leaf opening time-

1- 8:45am

2- 9:20am

3-9:40am

4-8:55am

sunrise time- 6:14am

Temperature - 29°/26°C

location- Panvel.

data collected by- Shraddha.

[9:09 PM, 8/2/2021] Shraddha CUBE: Continuous light experiment-

day 2 (2/8/21)

plants transferred under continuous light 31/07/21(8:30pm)

leaf opening time-

1- 8:37am

2- 8:07am

3-8:25am

4-7:48am

sunrise time- 6:14am

Temperature - 29°/26°C

Intensity of light- 545 Lux

location- Panvel.

data collected by- Shraddha.

[9:10 PM, 8/2/2021] Shraddha CUBE: Control set up

leaf opening time-

1.6:50am

2.7:05am

3.6:55am

4.7:01am

sunrise time- 6:14am

temperature- 29°/26°C

Intensity of light- 916Lux

day length- 12.57.43 Sunrise and sunset times in Panvel

[9:10 PM, 8/2/2021] Shraddha CUBE: Control set up-

day 2(2/8/21)

leaf closing time-

1- 6:27pm

2- 6:10pm

3-6:47pm

4-6:59 pm

sunset time- 7:12pm

temperature-29°/26°C

intensity of light-97 Lux

location- Panvel.

data collected by- Shraddha.

[9:27 PM, 8/2/2021] Jai Kishan CUBE: We are discussing Epigenetics in Pagalapos

[9:27 PM, 8/2/2021] Jai Kishan CUBE: Can environmental changes in Pagalapos result in epigenetic changes in microbes?

[9:27 PM, 8/2/2021] Jai Kishan CUBE: Please join Chatshalla

[KiranKalakotiR (Kiran Kalakoti)]

2nd August 9.30pm to 3rd August 9.30pm

[9:37 PM, 8/2/2021] Batul Pipewala Kolkata: If we compare this data with leaf closing time how much is their daylength meaning leaf open duration.

If we do the same calculations for pots in a dark do we see a pattern?

[9:50 PM, 8/2/2021] Shraddha CUBE: Continuous light experiment-

day 2(2/8/21)

plants transferred under continuous light 31/07/21(8:30pm)

leaf closing time-

1- 8:24pm

2- 7:39pm

3-7:39pm

4-7:47 pm

intensity of light- 243Lux

location- Panvel.

temperature-29°/26°C

data collected by- Shraddha.

[10:00 PM, 8/2/2021] Shraddha CUBE: Continuous dark experiment-

day 2(2/8/21)

plants transferred under continuous dark 31/07/21(8:30pm)

leaf closing time-

1- 6:20pm

2- 6:10pm

3-8:25pm (older lvs partially

closed)

4-5:15pm

intensity of light- 0 Lux

location- Panvel.

temperature-29°/26°C

data collected by- Shraddha. In plant 3 - yonger leaves closed but most of older leaves was open @8:30pm. Whereas in other plants older + younger leaves fully closed

[10:10 PM, 8/2/2021] Batul Pipewala Kolkata: Are all 4 plants of same species?

[10:18 PM, 8/2/2021] Sunita Chahar: So circadian rythmn changed on the second day .

[10:19 PM, 8/2/2021] Sunita Chahar: Keep us updated daily

[10:55 PM, 8/2/2021] Saida Cube Rotifers: 501th Day of CUBE Chatshaala: 2nd August

https://www.google.com/maps/d/edit?mid=1PXyWOjNidOePjVua7lMLbWhoHggYjIhS&usp=sharing

30 CUBists from 19 Centres had joined the webinar

INDIA

Assam

Jorhat : Ishita Sonowal

Delhi

New Delhi : Raj Yashwantrao Patil

Kerala

Thriprayar : Aswathy Suresh

Kandassankadavu : Lakshmy PJ

Pazhuvil, Trissur : Seethalakshmi CR

Maharashtra

Kalyan : Aditya Joshi

Thane : Anshu Kadam

Breach Candy, South Mumbai : Arunan MC

Maharashtra : Divya Rao

Adarsh Vidyalaya, Mumbai : Gautam Mourya

Worli, Mumbai : Isha Pawle

Mumbai : Jaikishan Advani

Wardha : Janhvi Deshmukh

Adarsh Vidyalaya, Mumbai : Karan Fulwariya

Powai : Lydia Mathew

Mulund, Mumbai : Priti Kanade

Dahanu Road, Palghar : Sachin Pradhan

Bandra, Mumbai : Saida Sayyed

Panvel : Shr…

[11:42 PM, 8/2/2021] Seethalakshmy CUBE: Sir, i think these are crane flies but iam not sure about it

[11:46 PM, 8/2/2021] Seethalakshmy CUBE: Ohh i see.

In Kerala some ants have local name. Like ikkiliurumbu, panchasaaaraurumbu, kuniyan, Puliyurumbu, kadiyan urumbu etc …these are commonly seen in Kerala.

What i am understand from it’s local name is depends upon their habitat or behavior etc …

That is behind it’s naming.

[11:47 PM, 8/2/2021] Seethalakshmy CUBE: @Arunan HBSCE sir

[12:01 AM, 8/3/2021] Shraddha CUBE: Yes mam

[7:47 AM, 8/3/2021] Shraddha CUBE: Control set up Date- (Day3) 03/08/21

leaf opening time-

1.6:47am

2.6:54am

3.6:59am

4.7:04am

sunrise time- 6:15am

temperature- 29°/25°C

Intensity of light- 21Lux

day length- 12.56.55 Sunrise and sunset times in Panvel

[7:48 AM, 8/3/2021] Shraddha CUBE: Continuous light experiment-

day 3 (3/8/21)

plants transferred under continuous light 31/07/21(8:30pm)

leaf opening time-

1- 5:58am

2- 5:50am

3-5:45am

4-6:28am

sunrise time- 6:15am

Temperature - 29°/25°C

Intensity of light- 545Lux

location- Panvel.

data collected by- Shraddha.

[8:00 AM, 8/3/2021] Abhijeet Singh: Spider taking shelter beneath leafs during rain. 3rd Aug 2021. Ulwe, Navi Mumbai.

[8:19 AM, 8/3/2021] +91 50803: wow!! beautiful capture.

[8:22 AM, 8/3/2021] P. Chitralekha: when do the leaves begin to open?

[8:52 AM, 8/3/2021] Shraddha CUBE: Around 4:55 am leaves begin to open

[8:55 AM, 8/3/2021] Shraddha CUBE: Continuous dark experiment-

day 3 (3/8/21)

plants transferred under continuous dark 31/07/21(8:30pm)

leaves opening time-

1- 8:18am

2- 8:26am

3-7:52am

4-7:43am

sunrise time- 6:15am

Temperature - 29°/25°C

location- Panvel.

data collected by- Shraddha.

[9:03 AM, 8/3/2021] Arunan HBSCE: Please give a summary of your current research on Phyllanthus model system, with clear statement of the objective, please. @Shraddha CUBE so that all will start responding to you and can become collaborators in this eminently exciting research question. @Shraddha CUBE @Sunita Chahar @Khan Shaheen @Cutest_Soul_❤️ @Lakshmi PJ@Aditya Joshi and others.

[9:03 AM, 8/3/2021] Arunan HBSCE: Yesterday we had a reintroduction of Pagalapos thanks to such recent photos of Drainage Cover slabs with depressions that can collect water during rains or gardening etc.!

Let’s following up on it!

@Sukriti CUBE Mango @Saida Cube Rotifers among others contributed to get back to the Cubist’s discovery of Pagalapos!

Please do a google seach with key word Pagalapos!

Exclusive CUBE sites prop up!

[9:03 AM, 8/3/2021] Arunan HBSCE: This context gave ample scope to reintroduce concept map on Habitat, Biodiversity in a crevice of less than 10 cm diameter, variants, mutation, selection, Evolution in a tea-cup etc.

A lot of insecurities in understandings in all of us, being surfaced! Let’s follow up. @Saida Cube Rotifers @Sukriti CUBE Mango @Saida Cube Rotifers @Arunima SN Nattika Cardamine @Lakshmi PJ@Batul Pipewala Kolkata and orhers

[9:04 AM, 8/3/2021] Arunan HBSCE: Any simple research question in mind, like why this time of the year? Does cuckoo’s call happen in other areas in India in first half of August?

[9:06 AM, 8/3/2021] Arunan HBSCE: @Seethalakshmy CUBE

[9:12 AM, 8/3/2021] Arunima SN Nattika Cardamine: Date:3/08/21.Time: 9 am.Cardamine plant growing in the pot.it is a power full model system we can do genetic engineering. (floral dip method). These cardamine is brought from high altitude region Munnar and introduced to a low altitude region Kozhikode. Arunima CUBE KOZHIKODE KERALA.

[9:20 AM, 8/3/2021] Arunan HBSCE: @Arunima SN Nattika Cardamine Great to see this High altitude Cardamine growing in low altitude.

Please give some essential details on dates, altitudes, how easy or difficult to cultivate etc. @Arunima SN Nattika Cardamine

Are there any Control Cardamine from coastal area?

[9:22 AM, 8/3/2021] Arunan HBSCE: How do we connect the context of cultivation of Cardamine to Curricular & Syllabus topics of Horticulture?

@Arunima SN Nattika Cardamine @Aswathy Suresh CUBE @Lakshmi PJ@Ishita Assam @KIRAN CUBE @Shyam T.G Cube SN Nattika Kerala

[9:37 AM, 8/3/2021] Arunan HBSCE: Follow up please! @Janhvi Deshmukh

[9:39 AM, 8/3/2021] Arunan HBSCE: @Ujaan Banerjee Please post close up and clear photos.

[9:41 AM, 8/3/2021] Arunan HBSCE: @VishnupriyaFollow up please!

[9:42 AM, 8/3/2021] Arunan HBSCE: Please give details of these CUBE HomeLab Panvel Cardamines @Shraddha CUBE

[9:45 AM, 8/3/2021] Janhvi: I seen larve on 1/08/2021 on my open bait setup …now I am waiting for pupae …at second day I seen some big larve I think it’s a second insterlarve …

Location: wardha Maharashtra janhvi deshmukh homelab…

[9:48 AM, 8/3/2021] Arunan HBSCE: Testable Hypothesis by @Lakshmi PJ SN Nattika Phyllanthus

The amputated eye of the snail do not regenerate.

How do we test to verify or falsify this statement?

@Saida Cube Rotifers @AnjaniKashyap@Vishnupriya@Lakshmi PJ@Ishita Assam @Aastha Ahuja Cube

[9:54 AM, 8/3/2021] Arunan HBSCE: Any Ant model system collaborators to take this forward? @Seethalakshmy CUBE @Shrudhiga @Vishnupriya@Aditya Joshi @Sachin Pradhan@Raj Elphinstone Cube @Batul Pipewala Kolkata

[9:56 AM, 8/3/2021] Arunan HBSCE: What do we mean by variety of Butterfly classification? @Vishnupriya@Seethalakshmy CUBE @Dr. Sarita kumar Delhi CUBE

[9:57 AM, 8/3/2021] Arunan HBSCE: Please give updates on these Cardamines @Lakshmi PJ SN Nattika Phyllanthus

[9:58 AM, 8/3/2021] Janhvi: Handmaiden moth I seen in spinach growing in my pot… Janhvi deshmukh homelab bajaj collage of science wardha Maharashtra …

[9:59 AM, 8/3/2021] Arunan HBSCE: More documentation of Butterflies of CUBE Locality Lab Khopat, Thane ( W) @Abhijeet Singh

[10:01 AM, 8/3/2021] Arunan HBSCE: Confession of a Cardamine mentor continued Photo: mcarunan, CUBE HomeLab Sophia, South Mumbai Date: 13July 2021 Comment: Good to very good

[10:59 AM, 8/3/2021] Priti Kanade Cube Mumbai: Our cardamine can grow anywhere!

Even in a coconut shell

This is here from how much time?

How are you maintaining so many of them for such a long time?

[11:23 AM, 8/3/2021] SukritiYes, sir. It was really interesting to know that those relatively small depressions (isolated from each other by the concrete/metal base of the lids) are correlated with the islands (isolated from each other by the sea).

A question comes up in my mind: How the colonization of species gradually happened in these depressions?

Let’s say one of the lid is replaced today with a brand new one (but has the same design as the earlier lids). How will be the organisms colonizing this new place? What are the basic requirements of an organism to survive in a new place?

[11:32 AM, 8/3/2021] Arunan HBSCE: Are we expecting the same profile of organisms in the depressions, once we replace an old man-hole lid with a new one? @Sukriti CUBE Mango Why?

[11:33 AM, 8/3/2021] Arunan HBSCE: Cardamine culture 40 days ago, on 19 June 202. Confession of a Cardamine mentor to be continued below Photo: mcarunan, CUBE HomeLab Sophia, South Mumbai. Date: 19 June 2021 Comment on upkeep: Good

[11:35 AM, 8/3/2021] Arunan HBSCE: @Priti Kanade Cube Mumbai for your curiosity!

[11:36 AM, 8/3/2021] Arunan HBSCE: Confession of a Cardamine mentor Photo: mcarunan, CUBE HomeLab Sophia, South Mumbai. Date: 29July 2021 Comment: After approximately, 40 days of germination, Satisfactory to Good, but could be maintained better by photographing for Daily update and hence through daily caring! Jai ho!

[11:36 AM, 8/3/2021] Arunan HBSCE: @Priti Kanade Cube Mumbai for your curiosity! And ofcourse, for all others!

[11:44 AM, 8/3/2021] Arunan HBSCE: To a similar question when asked to the Jumping Gene fame Nobel winner Barbara McClintock on her model system Maize plants she apparently said, “I just ask the plants, and they tell me the story”.

@Priti Kanade Cube Mumbai

So, I just asked the Cardamine seeds first and then the plants; they told me what they wanted!

That’s all!

[11:46 AM, 8/3/2021] SukritiThe new lid depression will be colonized by the organisms who are living in the nearest old lid depression.

I would not say that the exact same profile of organisms will be there from the start. Because habituation in a already established ecosystem and in a totally new ecosystem is different. There might be some early colonisers followed by some late colonisers, who will gradually acquire their niches in their newly formed habitat.

So with time, they might show the same profile of organisms if both the depressions are maintained under the same environmental conditions.

@Arunan HBSCE sir

[12:10 PM, 8/3/2021] Arunan HBSCE: An interesting research question and a doable one,indeed.

Perhaps, starting with why some Pagalapos wells are preferred by mosquitoes, and not others? @Sukriti CUBE Mango @Saida Cube Rotifers @KIRAN CUBE

[12:21 PM, 8/3/2021] P. Chitralekha: And in control?

[12:43 PM, 8/3/2021] Arunan HBSCE: Crow kid is getting pampered! Mind you, the parent looks not that happy. Please comment. 3August,2021 CUBE HomeLab Sophia, Bhulabhai Desai Road, Mumbai Photo: mcarunan

[12:48 PM, 8/3/2021] Arunima SN Nattika Cardamine: Date:3/08/21.time :12 20pm.cardamine growing like a weed in between aloe vera.Arunima CUBE KOZHIKODE KERALA.

[12:49 PM, 8/3/2021] Arunan HBSCE: Wow! @Janhvi Deshmukh

Reference please! How do we know this is Handmaiden moth?

On what ground we assume that it is a moth and not a wasp? @Janhvi Deshmukh

[12:51 PM, 8/3/2021] Arunan HBSCE: I can s e another weed almost looking like Phyllanthus, on the left?

Any idea, what is it?@Arunima SN Nattika Cardamine

[12:52 PM, 8/3/2021] Arunan HBSCE: That plant looks sleeping even at 12:20 pm! @Arunima SN Nattika Cardamine

[1:01 PM, 8/3/2021] Janhvi: Syntomoides imaon - Wikipedia

[1:02 PM, 8/3/2021] Janhvi: @Arunan HBSCE sir

[1:04 PM, 8/3/2021] Shraddha CUBE: In Control- from 6:20 am

[1:05 PM, 8/3/2021] SukritiMosquitoes prefer these places for their breeding, right.

But the amount of water stored in these depressions is quite less. It might get evaporated soon (if continuos rainfall is absent). Then what will happen to those eggs? It might lead to a significant downfall in offspring survivability.

It seems that mosquito does not care about it. In other words, they show no such parental care for their eggs and offsprings.

[1:18 PM, 8/3/2021] Shraddha CUBE: Two days data of continuous light & Dark experiment in Phyllanthus urinaria plants-date of photo- 03/08/21 location- Panvel. Data collected by-Shraddha

[1:18 PM, 8/3/2021] Shraddha CUBE: Two days data of continuous light & Dark experiment in Phyllanthus urinaria plants date of photo- 03/08/21 location- Panvel. Data collected by-Shraddha

[1:18 PM, 8/3/2021] Shraddha CUBE: Two days data of continuous light & Dark experiment in Phyllanthus urinaria plants date of photo- 03/08/21 location- Panvel. Data collected by-Shraddha

[1:27 PM, 8/3/2021] Batul Pipewala Kolkata: V interesting data,if you can put the control data in same table it is easier to compare.

The ones in the dark on the first day were not affected they had approx 12hr day(leaves open) and night(leaves closed).On 2nd day however the leaves were open for lesser time than control.

[1:28 PM, 8/3/2021] Shraddha CUBE: @Lakshmi PJ@Janhvi Deshmukh @Aditya Joshi @Arunan HBSCE Sir @KIRAN CUBE mam @Dr Chun Mei Chang mam @Priti Kanade Cube Mumbai @Shyam T.G Cube SN Nattika Kerala Sir @Batul Pipewala Kolkata mam @Sunita Chahar mam @P. Chitralekha mam @Usha Mukundan mam & Others…

[1:30 PM, 8/3/2021] Batul Pipewala Kolkata: With continuous light as expected the leaves closed later than control on the first day

[1:35 PM, 8/3/2021] Shraddha CUBE: Ok mam, I will try to put control data also in same table.

[1:37 PM, 8/3/2021] Batul Pipewala Kolkata: Five days after set up Nematodes multiplying on raw potato.3/8/21Homelab Kolkata Batul

[1:38 PM, 8/3/2021] Batul Pipewala Kolkata: Two days after transfer of nematodes no movement of nematodes seen on rava sugar vinegar medium 3/8/21Homelab Kolkata Batul

[1:52 PM, 8/3/2021] Batul Pipewala Kolkata: WAVE LIKE MOTION OF NEMATODES ON POTATO SURFACE WITH BACTERIAL GROWTH HOME LAB KOLKATA BATUL3/8/21.Magnification using 10X magnifying glass and camera zoom 4X

[1:57 PM, 8/3/2021] Batul Pipewala Kolkata: Size of the nematode approx 2mm

[2:11 PM, 8/3/2021] Arunan HBSCE: Please write a short summary for all to understand your finding @Shraddha CUBE

[2:13 PM, 8/3/2021] Arunan HBSCE: Any reference? @Sukriti CUBE Mango

Will be very interesting to learn how parental care is relevant or not for a particular species of animals, like mosquitoes! @Sukriti CUBE Mango @Batul Pipewala Kolkata

[2:15 PM, 8/3/2021] Arunan HBSCE: Not clear! Please write a short summary of your results. @Shraddha CUBE

[2:16 PM, 8/3/2021] Shraddha CUBE: Ok Sir

[2:19 PM, 8/3/2021] Arunan HBSCE: When do we know that these are ready to harvest?

[2:26 PM, 8/3/2021] Arunan HBSCE: This is the passion fruit plant grown on fourth floor balcony giving fruits. 3 CUBE Sophia Bhulabhai Desai Road, South Mumbai 3 August 2021. This plant is a gift from a cubist Sulekha Sudhakaran of CUBE Social Mechanics, Thiruvananthapuram Photo: mcarunan

[2:31 PM, 8/3/2021] SukritiSure, sir. I will search for references and comment back soon.

[2:31 PM, 8/3/2021] Shyam T.G Cube SN Nattika Kerala: It will become yellowish in colour, when ready for harvest

[2:38 PM, 8/3/2021] Arunan HBSCE: MOSQUITO ovitrap set up by class 10 vaibhavi chaurasia.

Dated 2.08.21

Location - kanpur

Objective- MOSQUITO awareness program.

Study of lifecycle. Sent by Hina CUBE Kanpur

[3:05 PM, 8/3/2021] KIRAN CUBE: @Shraddha CUBE please join today’s CUBE chatshaala and let’s discuss your experiment and finding

[3:19 PM, 8/3/2021] Lakshmi PJ SN Nattika Phyllanthus: Why this control plants are wake and sleep up late @Shraddha CUBE

[3:30 PM, 8/3/2021] Janhvi: Crow are usually awake early in the morning because they are hungry…they are scavenger and look for dead things to eat like rats,bits of rotten meat… I think crow are hungry they shouting for food…@Arunan HBSCE sir…

[4:54 PM, 8/3/2021] Shraddha CUBE: Yes @KIRAN CUBE ma’am

[4:55 PM, 8/3/2021] Shraddha CUBE: @Lakshmi PJ this is not leaves wake & sleep up time.

data showing how many hours in day leaves open & remain closed in a night.

[5:06 PM, 8/3/2021] KIRAN CUBE: S4E133 CUBE chatShaala: "Pagalapos Islands"

Kindly click on the above👆🏻 given link to find out the summary of last discussion.

Invitation to join today’s CUBE chatShaala, Please add name of place/affiliation along with the FULL NAME while joining the Webinar

Here’s the direct link of the CUBE chatShaala webinar: https://webinar.hbcse.tifr.res.in/b/kir-vgc-6jf

Timing:5.30-9.30pm

Looking forward to your participation

Thanks.

[5:16 PM, 8/3/2021] Saida Cube Rotifers: Moina culture update

Cube home lab Bandra

Moina culture in the tank - (2l DC)

MT1 35 moina

MT2 25 Moina

MT3 27 Moina

Moina bottle culture - (125ml DC culture )

MB1 40 Moina

MB2 40 Moina

MB3 50 Moina

MB4 20 Moina

MB5 40 Moina

MB6 15 Moina

[5:19 PM, 8/3/2021] Batul Pipewala Kolkata: Are the number of moinas decreasing in the tank?

[6:05 PM, 8/3/2021] Janhvi: It’s a pupae

[6:06 PM, 8/3/2021] Janhvi: I seen some reddish type of larve on bait…

[KiranKalakotiR (Kiran Kalakoti)]

3rd August 9.30pm to 4th August 2021 9.30pm

[1:37 PM, 8/3/2021] Batul Pipewala Kolkata: Five days after set up Nematodes multiplying on raw potato.3/8/21Homelab Kolkata Batul
[1:38 PM, 8/3/2021] Batul Pipewala Kolkata: Two days after transfer of nematodes no movement of nematodes seen on rava sugar vinegar medium 3/8/21Homelab Kolkata Batul
[1:52 PM, 8/3/2021] Batul Pipewala Kolkata: WAVE LIKE MOTION OF NEMATODES ON POTATO SURFACE WITH BACTERIAL GROWTH HOME LAB KOLKATA BATUL3/8/21.Magnification using 10X magnifying glass and camera zoom 4X
[1:57 PM, 8/3/2021] Batul Pipewala Kolkata: Size of the nematode approx 2mm
[5:06 PM, 8/3/2021] KIRAN CUBE: S4E133 CUBE chatShaala: "Pagalapos Islands"
Kindly click on the above👆🏻 given link to find out the summary of last discussion.

Invitation to join today’s CUBE chatShaala, Please add name of place/affiliation along with the FULL NAME while joining the Webinar

Here’s the direct link of the CUBE chatShaala webinar: https://webinar.hbcse.tifr.res.in/b/kir-vgc-6jf

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
[10:00 PM, 8/3/2021] Aswathy Suresh CUBE: 502nd Day of CUBE Chatshaala: 3rd August 2021

24 CUBists from 23 Centres had joined the webinar

INDIA

Delhi
New Delhi : Raj Yashwantrao Patil
DSC, Delhi : Dr P. Chitralekha

Jharkhand
Kokar, Ranchi : Man Masih Beck
Morabadi, Ranchi : Rechel Tirkey

Kerala
Kandassankadavu : Lakshmy PJ
Pazhuvil, Thrissur : Seethalakshmi CR
Palakad : Shyam TG

Maharashtra
Thane : Anshu Kadam, Aditi Anup M
Breach Candy, South Mumbai : Arunan MC
Maharashtra : Divya Rao
Mumbai : Jaikishan Advani
Wardha : Janhvi Deshmukh
NES Ratnam College, Mumbai : Kareena Dhuri
Adarsh Vidyalaya, Mumbai : Karan Fulwariya
NES Ratnam College, Mumbai : Kareena Dhuri
Dahanu Road, Palghar : Sachin Pradhan
Bandr…
[12:32 PM, 8/4/2021] Batul Pipewala Kolkata: Nematode culture on raw potato 6days after isolation from soil.4/8/21Homelab Kolkata Batul
[12:43 PM, 8/4/2021] Batul Pipewala Kolkata: Tail end
[12:43 PM, 8/4/2021] Batul Pipewala Kolkata: Middle portion
[12:43 PM, 8/4/2021] Batul Pipewala Kolkata: Anterior end of nematode. Please help identify this soil nematode @Dr Arunan,@Anshu.This nematode belongs to Rhabditis family due to the presence of bulb and enlarged oesophagus,it is 2mm in length.Homelab Kolkata Batul 4/8/21
[5:02 PM, 8/4/2021] KIRAN CUBE: S4E134 CUBE chatShaala: CURD vs. CURDLING
Kindly click on the above👆🏻 given link to find out the summary of last discussion.

Invitation to join today’s CUBE chatShaala, Please add name of place/affiliation along with the FULL NAME while joining the Webinar

Here’s the direct link of the CUBE chatShaala webinar: https://webinar.hbcse.tifr.res.in/b/kir-vgc-6jf

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
[9:42 PM, 8/4/2021] Aswathy Suresh CUBE: 503rd Day of CUBE Chatshaala : 4th August 2021.

14 Cubists from 14 Centers had joined the webinar.

INDIA

Delhi
ANDC : Aastha Ahuja

Jharkhand
Kokar, Ranchi : Man Masih Beck
Morabadi, Ranchi : Rechel Tirkey

Kerala
Thriprayar : Aswathy Suresh
Kandassankadavu : Lakshmy PJ
Pazhuvil, Trissur : Seethalakshmi CR

Maharashtra
Ghatkopar, Mumbai: Aashutosh Mule
Ghatkopar: Anjani Kumar
Breach Candy, South Mumbai : Arunan MC
Worli, Mumbai: Isha Pawle
Panvel : Shraddha Sonavane

Uttar Pradesh
Moradabad : Kiran Yadav

West Bengal
Kolkata : Batul Pipewala

Naaz Khan

{Note : Cubists who have joined today but their name isn’t included in the list, kindly reply to this message.}

[KiranKalakotiR (Kiran Kalakoti)]

4th August 2021 9.30pm to 5th August 2021 9.30pm
[7:50 AM, 8/5/2021] Arunan HBSCE: Milk Curdling Biological vs Chemical What is the alternative when you see there is no milk at home, to start the experiment? How about using milk powder, to make milk and proceed? 4 August, 2021 CUBE HomeLab Sophia, Bhulabhai Desai Road, South Mumbai Photo: mcarunan
[7:52 AM, 8/5/2021] Batul Pipewala Kolkata: Dense Nematode population on raw potato medium 7days after isolation.Homelab Kolkata Batul 5/8/21
[8:09 AM, 8/5/2021] Arunan HBSCE: Curdling of Milk Biological vs Chemical process. A quick design at CUBE HomeLab, Sophia, Bhulabhai Desai Road, Mumbai 5:00 pm 4 August 2021 Photo: mcarunan
[8:14 AM, 8/5/2021] Arunan HBSCE: Curdling Studies Biological vs Chemical Milk with 3 drops each of Tap water, Curd, Lemon & Vinegar in bottle cap. After 2 hrs. of addition, on 4 Aug. 2021 Photo: mcarunan CUBE HomeLab Sophia, Bhulabhai Desai Road, South Mumbai.
[2:15 PM, 8/5/2021] Batul Pipewala Kolkata: Nematodes transferred today 5/8/21 to new raw potato slices A and B from earlier potato culture.Homelab Kolkata Batul
[5:01 PM, 8/5/2021] KIRAN CUBE: S4E134 CUBE chatShaala: CURD vs. CURDLING
Kindly click on the above👆🏻 given link to find out the summary of last discussion.

Invitation to join today’s CUBE chatShaala, Please add name of place/affiliation along with the FULL NAME while joining the Webinar

Here’s the direct link of the CUBE chatShaala webinar: https://webinar.hbcse.tifr.res.in/b/kir-vgc-6jf

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
[5:33 PM, 8/5/2021] Batul Pipewala Kolkata: SUMMARY OF ISOLATION AND CULTURE OF NEMATODES USING RAW POTATO started on 29/7/21
[5:34 PM, 8/5/2021] Batul Pipewala Kolkata: BREAKING NEWS NEMATODES SWIMMING AT THE EDGES OF A BACTERIAL POOL ON RAW POTATO SURFACE 48HRS AFTER SET UP Home lab Kolkata Batul
[5:35 PM, 8/5/2021] Batul Pipewala Kolkata: Nematodes growing well on the raw potato medium 1/8/21
[5:36 PM, 8/5/2021] Batul Pipewala Kolkata: 2/8/21 Nematodes on the potato
[5:36 PM, 8/5/2021] Batul Pipewala Kolkata: Five days after set up Nematodes multiplying on raw potato.3/8/21Homelab Kolkata Batul
[5:36 PM, 8/5/2021] Batul Pipewala Kolkata: Nematode culture on raw potato 6days after isolation from soil.4/8/21Homelab Kolkata Batul
[5:38 PM, 8/5/2021] Batul Pipewala Kolkata: Dense Nematode population on raw potato medium 7days after isolation.Homelab Kolkata Batul 5/8/21
[5:44 PM, 8/5/2021] Batul Pipewala Kolkata: Nematode pool on day 7 much bigger and surrounded by fungal growth. Pic 5/8/21 Homelab Kolkata Batul
[6:36 PM, 8/5/2021] Batul Pipewala Kolkata: Boiled potato slices with nematodes transferred to them.5/8/21Homelab Kolkata Batul
[10:02 PM, 8/5/2021] Aswathy Suresh CUBE: 504th Day of CUBE Chatshaala : 5th August 2021.

24 Cubists from 23 Centers had joined the webinar.

INDIA

Delhi
ANDC : Aastha Ahuja
DSC, Delhi : Dr P. Chitralekha
ANDC : Dr Sarita Kumar
ANDC Delhi : Subham

Goa
Goa : Ankit Yadav

Kerala
Thriprayar : Aswathy Suresh
Thanniam, Thrissur : Sidhy PP

Maharashtra
Thane : Anshu Kadam , Aditi Anup M
Ghatkopar: Anjani Kumar
Breach Candy, South Mumbai : Arunan MC
Maharashtra : Divya Rao,Jasmin Shaikh
Adarsh Vidyalaya, Mumbai : Karan Fulwariya
NES Ratnam College, Mumbai : Khan Shaheen, Sunita Chahar
Worli, Mumbai: Isha Pawle
Bandra, Mumbai : Saida Sayyed
Panvel : Shraddha Sonavane
Prabhadevi, Mumbai : Yash Sheregare

Tamil Nadu
PS…

[KiranKalakotiR (Kiran Kalakoti)]

5th August 2021 9.30pm to 6th August 2021 9.30pm
[11:56 AM, 8/6/2021] Batul Pipewala Kolkata: Interestingly the liquified potato plus microbial pool and hence nematodes are growing over and above the fungi at the border of the pool.6/8/21Homelab Kolkata Batul
[11:57 AM, 8/6/2021] Batul Pipewala Kolkata: Nematodes after 24hrs on new raw potato slice without soil or milk.6/8/21 Homelab Kolkata Batul
[12:46 PM, 8/6/2021] +91 03221: Mam in 24 hour you see nameatodes in raw potatoes @Batul Pipewala Kolkata mam
[12:51 PM, 8/6/2021] +91 03221: It’s looks so long … @Batul Pipewala Kolkata mam what the size of this nematodes mam…?
[12:51 PM, 8/6/2021] Batul Pipewala Kolkata: I had transferred nematodes yesterday from the earlier potato slice1 to a fresh potato slice
[12:52 PM, 8/6/2021] +91 03221: Ohh …it’s look long…
[12:52 PM, 8/6/2021] Batul Pipewala Kolkata: It is magnified using a 10X magnifying glass and 4X camera zoom.Its size is 2mm
[12:54 PM, 8/6/2021] +91 03221: Ok mam
[1:25 PM, 8/6/2021] +91 40128: Tomato was kept in open space in balcony. After 2 days…
[1:26 PM, 8/6/2021] +91 03221: I see some fruitfly fly …
[1:26 PM, 8/6/2021] +91 03221: With some microbial growth also
[1:26 PM, 8/6/2021] +91 40128: Yes their was upto 4 5 fruit fly in 11a.
[1:26 PM, 8/6/2021] +91 40128: 11Am
[1:26 PM, 8/6/2021] +91 40128: That’s may be fungus
[1:27 PM, 8/6/2021] +91 40128: It’s may be fungul growth
[1:27 PM, 8/6/2021] +91 03221: You trapped fruitsfly then?
[1:27 PM, 8/6/2021] +91 40128: Yes, as well fungus too
[1:28 PM, 8/6/2021] +91 03221: Ohh what is your hypothesis? @Radhakrishn Sakhi
[1:29 PM, 8/6/2021] +91 40128: Fruit flys trapped on moist food
[1:30 PM, 8/6/2021] +91 40128: As well fungul growth is mainy seen after 2 day
[1:34 PM, 8/6/2021] +91 03221: what was your objective ?? @Radhakrishn Sakhi
[1:35 PM, 8/6/2021] +91 40128: An tomato kept in open space
[1:40 PM, 8/6/2021] +91 03221: I mean what is your next plan ???
[1:40 PM, 8/6/2021] +91 40128: O sorry, actually iam in class
[1:42 PM, 8/6/2021] +91 03221: Yes
[2:05 PM, 8/6/2021] +91 03221: This is my open bait setup on 29/07/2021 I trapped 13,14 flies in my open bait setup in which I used tomato and cucumber I keep the setup in my backyard near the dishwashing area…where I trapped the flies…on 01/08/2021 I seen some larve moiving inside the bait …and 03/08/2021 I seen some pupue…but after sometime I seen some different types of larve you can see in the picture …what you think it’s from different fly ???@Aswathy Suresh CUBE @Batul Pipewala Kolkata mam @Arunan HBSCE sir @KIRAN CUBE mam…
[2:08 PM, 8/6/2021] +91 03221: When i see the bait today I got one fruitfly in the bait and I see some big larve convert into pupae its a pupae? @Aswathy Suresh CUBE @KIRAN CUBE @Arunan HBSCE sir @Batul Pipewala Kolkata mam
[2:09 PM, 8/6/2021] +91 03221: Janhvi deshmukh homelab bajaj collage of science wardha Maharashtra…
[2:10 PM, 8/6/2021] +91 03221: My collaborators @Aswathy Suresh CUBE @KIRAN CUBE @Yash Sheregare CUBE @Batul Pipewala Kolkata mam …
[2:39 PM, 8/6/2021] Aswathy Suresh CUBE: SUMMARY OF CUBE CHATSHAALA
05/08/2021

Concept map of MOINA

•Micro biology •Molecular biology •Biochemistry •Bacterial growth curve •Cell biology.

We often discuss bacterial growth curve whenever it comes to the concept of low level of oxygen.also we had the discussion around the differences and similarities in Chromosome, DNA, gene, protein,chromatids.discussion sometimes on what’s the body fluid in Moina…

What is the connection between Micro biology & Moina!?

Difference between Micro - Macro organisms! Examples.

Concept map is not complete yet. Please add your points.
[2:42 PM, 8/6/2021] Aswathy Suresh CUBE: SUMMARY OF CUBE CHATSHAALA.

SNAIL

@Subham from odisha was discussed about maintained setup of Gaint African snails from his college lab.

How to maintain snails!? What was the setup!

@Saida explained her home lab setup for maintain snails.

Feeding
This herbivore does not discriminate between living or dead plant matter. It has such an enormous appetite that it feeds on more than 500 types of plants, including those farmed by humans.

The Giant African Snail eats leaves, flowers, fruits, stems, barks, wood, seeds, grains, nuts, seaweed and even lichens, fungi and other snails. Economically valuable crops for humans such as cauliflower, cocoa, papaya, peanut, cassava, banana and m…
[2:42 PM, 8/6/2021] Aswathy Suresh CUBE: CARDAMINE

Discussion about ;

How can we identify different species of Cardamine looking their morphology features?

Which morphology part need to look for identifying at species level? Flowers, Leaves, Stamens etc

What is crucifrom?
So cruciform is about petal shape!

Brassicaceae (/ˌbræsɪˈkeɪsii/) or Cruciferae (/kruːˈsɪfəri/)[2] is a medium-sized and economically important family of flowering plants commonly known as the mustards, the crucifers, or the cabbage family. Brassicaceae - Wikipedia
Cruciform means having the shape of a cross or Christian cross. Cruciform - Wikipedia
I also didnt know what is cruciform and its meaning… So this helped…

Is it shape or arrangement?

https://www.sciencephoto.com/media/…
[5:10 PM, 8/6/2021] KIRAN CUBE: S4E136 CUBE chatShaala: " Identification of different species of Cardamine"

Kindly click on the above👆🏻 given link to find out the summary of last discussion.

Invitation to join today’s CUBE chatShaala, Please add name of place/affiliation along with the FULL NAME while joining the Webinar

Here’s the direct link of the CUBE chatShaala webinar: https://webinar.hbcse.tifr.res.in/b/kir-vgc-6jf

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
[5:31 PM, 8/6/2021] Batul Pipewala Kolkata: HOLD YOUR BREATH!!You see here the developing nematode embryo.Homelab KolkataBatul 6/8/21 Video under 45X of compound microscope
[5:37 PM, 8/6/2021] Batul Pipewala Kolkata: Three nematode embryos twisting and turning at the centre.video under 10X of compound microscope. Homelab Kolkata Batul 6/8/21
[5:42 PM, 8/6/2021] CUBist: Within 6 hours fungul attack

[KiranKalakotiR (Kiran Kalakoti)]

6th August 2021, 9.30pm to 7th August 2021, 9.30pm
[5:42 PM, 8/6/2021] +91 40128: Within 6 hours fungul attack
[6:10 PM, 8/6/2021] Aswathy Suresh CUBE: Phyllanthus discussion going on. Shraddha sharing her continuous light experiment in phyllanthus plants…

Please join everyone @Arunan HBSCE sir @Saida Sayyed @⁨Arunima @⁨Lakshmi @Janhvi Deshmukh @⁨Adithya @Batul Pipewala Kolkata ma’am @⁨Shrudhika⁩ @Ishita Assam @Darshan Dudhane @Radhakrishn Sakhi
[6:59 PM, 8/6/2021] Janhvi Deshmukh: @Batul Pipewala Kolkata mam can tell the stages of embryogenesis in nematodes?
[7:03 PM, 8/6/2021] Janhvi Deshmukh: Embryo Introduction @Batul Pipewala Kolkata mam can you tell about the reference I found the reference but I can’t understand ??
[7:12 PM, 8/6/2021] Batul Pipewala Kolkata: Stages in embryogenesis of C elegans and larval development. Referencehttps://www.researchgate.net/figure/Life-cycle-of-C-elegans-at-20-C-An-adult-hermaphrodite-produces-roughly-300-eggs_fig3_265581569
[8:58 PM, 8/6/2021] Batul Pipewala Kolkata: Aim of the experiment : To study olfactory response and chemotaxis in nematodes.
[9:03 PM, 8/6/2021] Batul Pipewala Kolkata: Results: Nematodes moved to the curd at 8.30pm the set up was done at 3.0pm that is after 5hrs 30min.date 6/8/21Homelab Kolkata BatulP.
[9:20 PM, 8/6/2021] Janhvi Deshmukh: Mam what is this ??this is also a nematodes
[9:38 PM, 8/6/2021] Janhvi Deshmukh: @Radhakrishn Sakhi you can see larva here kajol …it’s a fruit fly larve may be fruitfly lay there egg on tomato can you keep it open …why don’t you try to close in the bottle or box ?

[KiranKalakotiR (Kiran Kalakoti)]

7th August 2021, 9.30pm to 8th August 2021, 9.30pm

Leaves are open at 12:40am in continuous dark set up date of photo-07/08/21. Location-Panvel.Leaves are open at 12:40am in continuous light set up date of photo-07/08/21. Location-Panvel.

All leaves are closed at 12:55 am in Control plants. date-7/8/21. Location- Panvel

One Seed germinated. It was observed on 5th day (5/8/21)in pot 3 of continuous light set up​:+1:
Wow! @Batul Pipewala Kolkata Wonderful!

Can’t we also take videos using mobile phones?!
[9:16 AM, 8/7/2021] Arunan HBSCE: What an impressive progress in HomeLab Nematode studies using Potato slice and curd!
Wow! @Anshu Kadam (Nematodes) What are you waiting for?
[9:16 AM, 8/7/2021] Arunan HBSCE: Lease look around! And ask this butterfly! It will take you to its host plants! @Shrudhiga
[9:16 AM, 8/7/2021] Arunan HBSCE: What is the magnification? @Batul Pipewala Kolkata
[10:55 AM, 8/7/2021] Arunan HBSCE: Please describe which type of pharyngeal shape or morphology, you are referring to. @Batul Pipewala KolkataBoth Caenorhabditis nematodes and LABs inhabit rotting fruit in nature
The most obvious source of citrate in nature is in fruits. Coincidently, one of the common places to find C. elegans and other nematodes is within rotting fruits (Felix and Braendle, 2010; Felix and Duveau, 2012). We wanted to confirm this previous finding locally in the Korean peninsula. We collected rotten fruit and adjacent soil from several fruit farms during each fruit harvest season. In the laboratory, we picked out nematodes from within these rotten fruit samples, and identified the nematodes by 18S rDNA sequencing. We successfully found Caenorhabiditis species such as C. remanei and C. briggsae in pear and yuzu fruit samples (Table 1). We also found other types of nematodes such as Panagrellus and Pristionchus that were also previously reported to be found in fruit (Felix and Duveau, 2012)."
A natural odor attraction between lactic acid bacteria and the nematode Caenorhabditis elegans | The ISME Journal
[10:55 AM, 8/7/2021] Arunan HBSCE: Great reference. Let’s understand the most relevant part!
[10:55 AM, 8/7/2021] Arunan HBSCE: Wow!
Let’s do it with replicates and Controls! A great HomeLab version for Nematodes including C elegans! @Batul Pipewala Kolkata
[11:29 AM, 8/7/2021] Batul Pipewala Kolkata: Thank you Sir,will do it with controls again
[11:30 AM, 8/7/2021] Batul Pipewala Kolkata: Magnification is under 10X of compound microscope
[11:34 AM, 8/7/2021] Batul Pipewala Kolkata: A broad oesophagus and bulb like pharynx are features of family Rhabtidae
[11:37 AM, 8/7/2021] Arunan HBSCE: Please discuss about the Design of that experiment in detail, so that others can add on it as well. @Batul Pipewala Kolkata
Let’s all learn together, how to DESIGN a good experiment, collaboratively! @Batul Pipewala Kolkata
[11:37 AM, 8/7/2021] Arunan HBSCE: 10X objective, right?
What was the eye piece magnification?
[11:38 AM, 8/7/2021] Arunan HBSCE: In that case, what is the size of the eggs and embryos as found by you, yesterday? @Batul Pipewala Kolkata
[11:39 AM, 8/7/2021] Arunan HBSCE: Photo of a tough diagram please! @Batul Pipewala Kolkata @Anshu Kadam (Nematodes)
[11:39 AM, 8/7/2021] Arunan HBSCE: rough*

Fruitfly near the washbasing … janhvi deshmukh homelab bajaj collage of science wardhaSize of the nematode embryo is approx 200microns
Doubt: Do bacteria don’t grow on potato?
How long nematodes can be maintained on Potato?
200 micron is like a pointed dot and is 0.2 mm. 1/5th of a millimeter! Not bad!
[1:58 PM, 8/7/2021] Batul Pipewala Kolkata: Nematodes can multiply on the potato surface till the starch has been used up where they are growing.They continue to multiply on the same spot on the potato where there was microbial pool though rest of the surface is unoccupied
[1:59 PM, 8/7/2021] Batul Pipewala Kolkata: They can be maintained for 15 to 20days

Date:7/08/21.Time 2 10pm.Chlorosis is observed in the cardamine plant.Date:7/08/21.Time 2 10pm.Chlorosis is observed in the cardamine plant.SUMMARY - CUBE CHATSHAALA
6th August 2021

Phyllanthus Plants - Continuous Light/Dark Experiment.

Shraddha shared her data of continues Light/Dark (with control) experiment started in Panvel - Mumbai. Addressing research question Continues Light/Dark will be effecting sleep wake pattern of Phyllanthus plants.

Experiment taken plant was Phyllanthus urinaria.

4 plants each for Control, Continuous Light and Continuous Dark.

Plot one graph based on the 6 days data’s from morning & evening , when plant is started to waking and started to sleeping (during time)

Control Data is constant in this graph. Light/Dark showing some changes throughout every days.

What will be the effect of plants near Street lamps?

Comparing Circadian rhythms gene in Animals & Plants.

Example one article saying about Arabidopsis - circadian clock

•CIRCADIAN AND CLOCK ASSOCIATED1 (CCA1)
• LATE ELONGATED HYPOCOTYL (LHY)

These gene are effecting the circadian rhythm of plants.
@Lakshmy PJ CUBE @Sunita Chahar CUBE ma’am @P. Chitralekha Cube JNU ma’am please add your comments

A simplified version of the Arabidopsis circadian clock is illustrated in Figure 3 (see also Table 1). It comprises three interlocked feedback loops, with two single Myb domain transcription factors, CIRCADIAN AND CLOCK ASSOCIATED1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY), playing roles in each loop. TOC1, the founding member of a family of pseudo-response regulators (PRRs), closes one loop, while three TOC1 paralogs, PRR5, PRR7, and PRR9, close a second loop. A third loop includes a Myb transcription factor, LUX ARRHYTHMO (LUX).

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1425852/
Continues Light & Dark Experiment Done by @Sunita Chahar CUBE from Mumbai

Experiment started on 4th July 2021 with 3 pots. Taken Phyllanthus species - Phyllanthus amarus. Each pots have 4 plants. Still continuing this experiment…

Setup 1 (Pot 1) : Control
Setup 2 (Pot 1) : LED
Setup 3 (Pot 1) : Dark

Expectation : Delay the pattern on sleep wake cycle which are kept it in Light and Dark.

Observation : Phyllanthus plants are not closing their leaves which are kept in continues LED and Dark.

Conditions of plants;
LED kept plants : 1 plant is remains today. Other plants dried and yellowish colored.
Dark kept plants : Died.

Starting experiment time plants showed the delay of sleeping waking time but after 5 days later (10/07/2021) showed these plants are not closing leaves which are kept in continues dark and light period.

@Sunita Chahar CUBE ma’am please add your points

Similar kind of behavior started to observed @Shraddha Sonavane CUBE in her continues Light experiment setup also after 6th days later started experiment.

2 pots plants are not sleeping which are kept in LED at 9:30pm. Leaves are still opened in at 9:30pm.
Also 12:00am 4:00am checked these plants - still these plants were opened leaves.

Photo shared by Shraddha - 05/08/2021 ; 9:30pm.

One pot plant started to drying. Plants showing not healthy conditions. Watering every day morning time (Once in a day).

What could be the reason plants not started to closing leaves!? Is there any effect of Light!There must be some reason for ‘chlorosis’. Firstly, what do we mean by chlorosis?
Is it due to lack of essential nutrients including water?@Arunima SN Nattika Cardamine
How often, have you been watering daily? @Arunima SN Nattika Cardamine
Can we pinpoint the reason for this behavior of Cardamine? @Arunima SN Nattika Cardamine
Let’s Celebrate the Goof up! @Arunima SN Nattika Cardamine
To me, the trend in Waking up timing, in Continuous Light and Continuous Dark are similar!
What do you say? @Lakshmy PJ CUBE @Shraddha Sonavane CUBE @Sunita Chahar CUBE @Cutest_Soul_❤️ @Khan Shaheen @Aditiya Joshi CUBE and others
The trend of Sleeping time too shows similar trend till 4 th day for Continuous Light and Continuous Dark situations!
What do you say? @Lakshmy PJ CUBE @Shraddha Sonavane CUBE @Sunita Chahar CUBE
Can somebody summarise our understanding on circadian genes and their role in plants, starting with Arabidopsis? @Lakshmy PJ CUBE @Shraddha Sonavane CUBE k
@Sunita Chahar CUBE please give a summary from your point of view.There must be some reason for ‘chlorosis’. Firstly, what do we mean by chlorosis?
Is it due to lack of essential nutrients including water?@Arunima SN Nattika Cardamine
How often, have you been watering daily? @Arunima SN Nattika Cardamine
Can we pinpoint the reason for this behavior of Cardamine? @Arunima SN Nattika Cardamine
Let’s Celebrate the Goof up! @Arunima SN Nattika Cardamine
To me, the trend in Waking up timing, in Continuous Light and Continuous Dark are similar!
What do you say? @Lakshmy PJ CUBE @Shraddha Sonavane CUBE @Sunita Chahar CUBE @Cutest_Soul_❤️ @Khan Shaheen @Aditiya Joshi CUBE and others
The trend of Sleeping time too shows similar trend till 4 th day for Continuous Light and Continuous Dark situations!
What do you say? @Lakshmy PJ CUBE @Shraddha Sonavane CUBE @Sunita Chahar CUBE
Can somebody summarise our understanding on circadian genes and their role in plants, starting with Arabidopsis? @Lakshmy PJ CUBE @Shraddha Sonavane CUBE k
@Sunita Chahar CUBE please give a summary from your point of view.Plant : Phyllanthus urinaria.
The younger leaves of phyllanthus urinaria are different from older leaves, mainly in their size and shape( Heteroblasty)
Juvenile leaves include 2 cotyledons, followed by eophylls (up to 8 ).
a) cotyledons
b) eophylls ( young leaves).
Data collected by: Theertha M.D.
Place: Cherukkad, Kozhikode.
CUBE SN Nattika.

Changes from normal behaviour.
Plant : Phyllanthus urinaria.
Date:13/07/2021.
Place: Cherukkad, Kozhikode.
Time:4pm.
Data collected by :Theertha.
CUBE SN Nattika.

Changes from normal behaviour.
Finally the plant perished.
Place: Cherukkad, Kozhikode.
Data collected by: Theertha M.D.
CUBE SN Nattika.
Why this happens?
Insect attack!?Enviormental changes!? Mutation!?viral infections!? @Lakshmy PJ CUBE @Arunan HBSCE @KIRAN CUBE @P. Chitralekha Cube JNU and others.

Nematode larvae at different stages of development. Homelab Kolkata 7/8/21

Position of vulva is at the centre of my Rhabditidae nematodes.Homelab Kolkata 7/8/21

Position of vulva on my Rhabtidae nematodes. Homelab Kolkata 7/8/21

VIDEOS SHARED BY BATUL AT 4.46PM
BREAKING NEWS Nematodes growing on Rava sugar medium 24hrs after transfer of nematodes.
Nine days after set up flourishing nematode culture on raw potato slice7/8/21Homelab Kolkata BatulP
7/8/21Homelab Kolkata BatulP

Rava sugar medium without vinegar.The orange spots are.of saffron

Bacterial growth on boiled potato after 24hrs however nematodes transferred to it have not survived,they need a moist film on the surface whereas the potato surface was dry.Homelab Kolkata 7/8/21
Yes
So what we do next in this situation @Batul Pipewala Kolkata mam?
I have transferred nematodes to raw potato surface and am adding water to it .They have survived but they need to multiply
How is it remaining so wet? How did you add nematodes?
And how many did you add?
Excellent! @Batul Pipewala Kolkata Please give the date of setting up along with the date of photo.@Batul Pipewala Kolkata
Please give details of dates and how many nematodes transferred from which source? @Batul Pipewala Kolkata
I add a drop of water before taking the pic as it makes it more clear,other than that no water is added to it.The wetness is as the potato is getting liquified where nematodes are growing
Please join Chatshaala for discussion on the brilliant HomeLab Nematode culture pictures, by Batul CUBE Kolkata.
[6:10 PM, 8/7/2021] Batul Pipewala Kolkata: A few nematodes from the original potato slice on which nematodes were isolated from soil.Date of set up yesterday 6/8/21
[6:11 PM, 8/7/2021] Batul Pipewala Kolkata: Date of set up 29/7/21, date of photo 7/8/21
[6:11 PM, 8/7/2021] Batul Pipewala Kolkata:
Wow @Batul Pipewala Kolkata plenty of nematodes.
Wow It is really amazing
@Batul Pipewala Kolkata

VIDEO SHARED BY BATUL AT 7.58PM
Great design and discussion on HomeLab isolation of C elegans & it as a nematode in decaying fruits than just a mere soil nematode!
Please join in collaboratively designing HomeLab experiments!
[8:07 PM, 8/7/2021] Priti Kanade: +1
[8:09 PM, 8/7/2021] Priti Kanade:
[8:10 PM, 8/7/2021] Priti Kanade: Abhijeet can you resharw
I missed it
[8:10 PM, 8/7/2021] Priti Kanade: I’m also having bittergourd
Got it
[8:12 PM, 8/7/2021] Priti Kanade: Here
[8:39 PM, 8/7/2021] Priti Kanade: Interesting thing is how they manage to find any source even though they can’t see
[8:40 PM, 8/7/2021] Priti Kanade: Awaiting response
[8:40 PM, 8/7/2021] Priti Kanade: +
Interesting
How these insects camouflage with plants?

[9:38 PM, 8/7/2021] Priti Kanade: That’s great
I actually couldn’t understand
Is it the egg we are seeing?
[9:38 PM, 8/7/2021] Priti Kanade: I don’t have much idea about nematode embryo
Yes we are seeing the tiny worm growing inside the egg

[KiranKalakotiR (Kiran Kalakoti)]

8th August 2021, 9.30pm to 9th August 2021, 9.30pm

A phylogeny and molecular barcodes for Caenorhabditis, with numerous new...

key additional reason for the lack of known diversity in the Caenorhabditis genus is that these so-called “soil nematodes” are rarely found in soil samples. Soil samples yield a variety of nematode species, including a few species of the family Rhabditidae (to which Caenorhabditis belongs). For example, some Oscheius species are readily found in soil samples [15]. However, despite extensive sampling for many years, we failed to isolate Caenorhabditis from soil [16]. Rare positive instances correspond to soil of orchards (e.g. C. elegans strain JU258, Madeira 2001), or soil below trees with rotting fruits (C. sp. 5 JU727). Instead, C. elegans, C. briggsae and C. remanei were found in compost heaps containing decaying vegetal material.
@Aswathy Suresh CUBE @Isha Pawle @Shraddha Sonavane CUBE @Anshu Kadam (Nematodes)

plant1, 2,3- leaves open from 05/8/21 in continuous dark. date of photo-- 7/8/21 time-9:54pm
location- Panvel
photo by- Shraddha[10:37 PM, 8/7/2021] Seethalakshmy CUBE: Sir, the right side pot i kept some sugar.
The ants like sugar that’s why right side pot contains more ants.
[10:37 PM, 8/7/2021] Seethalakshmy CUBE: Left one is water
[10:41 PM, 8/7/2021] Seethalakshmy CUBE: This is our Formicarium
Formicarium is used to culture the ants.

Date of setup : 24/5/21
Location : pazhuvil
Time :10:00am

materials required for formicarium

*plastic /glass jar
*Soil and stones
*two bottle caps
(1.Sugar
2. Water)

How do make
*we need plastic /glass jar (here i am using 34cm length jar)
*Put some soil and stones to repeat this process
Now the jar filled
*take two lids of bottles
Take sugar and water and kept on the jar,
Wait for ants.
After these setup i got some ants.
Immediative Objective : To culture the ants

Research question : How do ants communicateLet’s find out whether the ants you have have in this set up, are of single species or not?
How do we know this?@Seethalakshmy CUBE
Have you found out?
What about at later time?
Do they behave like that till next day morning? @Shraddha Sonavane CUBE
What is your observation?
How does it compare with that of @Sunita Chahar CUBE and collaborators?
Wonderful!
What does mealy bug look like? How is it different from the black any seen in the same picture?@Shrudhiga

Which ant is this and what is it doing on this brinjal plant?
Did the butterfly tell you which plants it visits?@Shrudhiga
[8:01 AM, 8/8/2021] Arunan HBSCE: Which plant is this? @Shrudhiga
Was the butterfly looking for nectar here or trying to lay eggs on leaves? @Shrudhiga Or, just wandering around looking for its favourite plants?@Shrudhiga @Shraddha Sonavane CUBE

Does this tell
[8:04 AM, 8/8/2021] Arunan HBSCE: I guess, on mosquito larvae, we may see clear segmentation! What do you say? @Yash Sheregare CUBE
But, in another insect larva like that of fruitfly do we see such segmentation? @Yash Sheregare CUBE @Aswathy Suresh CUBE @Aastha Ahuja Cube @Dr. Sarita kumar Delhi CUBE
[8:05 AM, 8/8/2021] Arunan HBSCE: Updates on Fruitflies of HomeLab Wardha, please! @Janhvi Deshmukh
[8:07 AM, 8/8/2021] Arunan HBSCE: How is other fruitfly cultures in TRSV medium, different from this culture ? @Yash Sheregare CUBE @Aswathy Suresh CUBE @Lydia cUBe Drosphila @Aastha Ahuja CubeWow! 12 Day old culture from a single mother fruitfly, shows so many adult flies in 12-13 days?!

Why do you think you were able to achieve this?
Or, is it normally expected? @Yash Sheregare CUBE @Aswathy Suresh CUBE @Janhvi Deshmukh
[8:17 AM, 8/8/2021] Arunan HBSCE: Please do not disturb the bottle much! @Yash Sheregare CUBE Flies would know how to negotiate!
[8:21 AM, 8/8/2021] Arunan HBSCE: What chemical changes causes milk to curdle when Lemon juice is added?
And when inoculum from previous curd is added? @Janhvi Deshmukh @Aastha Ahuja Cube @Ishita Assam @Lakshmy PJ CUBE @Arunima SN Nattika Cardamine @Shraddha Sonavane CUBE and others.
[8:24 AM, 8/8/2021] Arunan HBSCE: Looking for more follow up photos from other parts of Mumbai and rest of India and Srilanka @Aditiya Joshi CUBE
Please coordinate with others @Aditiya Joshi CUBE
[8:25 AM, 8/8/2021] Arunan HBSCE: Does Lactic acid break the structure of Casein protein? @Janhvi Deshmukh Please describe. @Seethalakshmy CUBE @Lakshmy PJ CUBE @Ishita Assam @Aastha Ahuja Cube
[8:27 AM, 8/8/2021] Arunan HBSCE: Looking for photo updates on this set up, after 6th August. @Batul Pipewala Kolkata
[8:30 AM, 8/8/2021] Arunan HBSCE: Looks like insects with two prominent eyes! @Batul Pipewala Kolkata Apologies for a not do clear photo!
Can curry leaf leaflets have axillary buds?

Or some insect camouflaging to escape predators? In the bargain giving curry leaves a different morphological identity?

Is ant getting fooled too? @Dr. Sarita kumar Delhi CUBE @P. Chitralekha Cube JNU @Lakshmy PJ CUBE @Shraddha Sonavane CUBE @KIRAN CUBE and others

What is our guess? Are these two insects on the curry plant? Apologies for not so clear a photo.Reposting for comments. Photo: mcarunan CUBE Sophia, Bhulabhai Desai Road, South Mumbai.
Follow up pictures, please. @Cutest_Soul_❤️
Looking for follow up photos @Abhijeet Singh
Please post follow up on this set up with photos @Batul Pipewala Kolkata
Fantastic astic photos! @Theertha CUBE What is your objective?
Interesting @Theertha CUBE

What is your guess?
What was the watering schedule?
Where exactly the set up was kept? Outside the room or inside?
Was in the dark or in continuous light.
Please give all essential information.
Updates on Rava medium, please @Batul Pipewala Kolkata

This is my open bait setup on 29/07/2021 I trapped 13,14 flies in my open bait setup in which I used tomato and cucumber I keep the setup in my backyard near the dishwashing area…where I trapped the flies…on 01/08/2021 I seen some larve moiving inside the bait …and 03/08/2021 I seen some pupue…but after sometime I seen some different types of larve you can see in the picture …what you think it’s from different fly ???@Aswathy Suresh CUBE @Batul Pipewala Kolkata mam @Arunan HBSCE sir @KIRAN CUBE mam…

See some red colour pupaeCasin can easily seperated from milk by making the milk more acidic …the lactic acid that is produced as a by-product makes milk more acidic…the lump that form when the milk is acidified are the casien Protein (curd)…@Arunan HBSCE sir…

Bacterial growth on boiled potato after 24hrs however nematodes transferred to it have not survived,they need a moist film on the surface whereas the potato surface was dry.Homelab Kolkata 7/8/21

MOSQUITO prevention and control. Water accumulation are probable breeding sites of these vectors. WHAT ARE VECTORS? Check out for eggs ,larvae, pupae… eradicate these water potholes ,to prevent spread of these MOSQUITO vectors. @ hina ,rahil cl 11cube kanpur . Date 8. 08.21
Pothos planters, can we find some MOSQUITO breeding here. Rain water accumulation is a favourite site for MOSQUITO breeding. Prevention is better than cure, avoid collection of water in and around your surroundings. To check out this disease causing vector to breed. @ hina, rahil class11 cube kanpur. Dated 8.08.21

Can we get some idea about this patrolling ant? Hope, this is ant! Apologies for the poor quality of the picture. @Seethalakshmy CUBE @Shrudhiga @Lakshmy PJ CUBESir, I think if there was any white mealybug in that plant then there is a possibility that the ants will arrive as mealybugs deliver a source of nutrition for ants…

VIDEO SHARED BY BATUL AT 9.26AM
Nematodes are multiplying on rava sugar medium 48hrs after transfer from original potato slice.The reddish colour is due to saffron added to rava.Homelab Kolkata Batul P 8/8/21
[9:28 AM, 8/8/2021] +91 22237: Sir, I think it would be drinking nectar as it is wandering around and sitting on a plant for some time…
[9:29 AM, 8/8/2021] +91 22237: Impatiens balsamina https://g.co/kgs/DMorsG

This was the plant which my butterfly was spotted…
Please name the plants, at least a few! @Shrudhiga

Sunday MOSQUITO surveillance by cubist from kanpur, MOSQUITO breeding sites. Water accumulation in and around RESIDENTIAL areas are probable breeding sites of these vectors. Dated 8.08.21 @ hina allen cube kanpur.
You still could not guess which butterfly this could be?
Any guess?
How do we do search to find using search terms?@Shrudhiga @Arunima SN Nattika Cardamine
Jamides celeno Jamides celeno - Google Search

This is my butterfly I think because it seems like that

VIDEO SHARED BY BATUL AT 9.37AM
Dense nematode culture on original raw potato slice 10days after set up.set up date 29/7/21 date of pic 8/8/21.No maintenance is needed.I add two drops of water before I take pics for clarity but they are not needed for their growth.Homelab Kolkata **
[9:37 AM, 8/8/2021] Arunan HBSCE: @Hina Mudgal Kanpur CUBE and fellow cubists, Great to see the initiative. Please post Daily updates to inspire cubists from other centers! Hope, after observing you will empty the vessel , as a public health conscious citizen science, program.

What types of mosquitoes and larvae your group expects in this? Why?
Please give the names of collaborators with the names of CuBE HomeLab.
[9:42 AM, 8/8/2021] Arunan HBSCE: Common name please!

What do you call these butterflies, in your locality? Don’t they have a name in the locality?@Shrudhiga
If not, why not?

Alert!!! Nematodes are NOT multiplying on new raw potato slice without soil set up in duplicates B and C and seeded with few nematodes from original slice three days ago.Again today nematodes have been added to B and C.2 to 3 drops of water is added to it to keep it moist.Are bacteria from soil important for their survival ?CUBE homelab Kolkata BatulP 8/8/21Pothos planter MOSQUITO breeding sites. SURVEILLANCE team works on Sundays, cube kanpur. 08.08 21
Pothos planters, can we find some MOSQUITO breeding here. Rain water accumulation is a favourite site for MOSQUITO breeding. Prevention is better than cure, avoid collection of water in and around your surroundings. To check out this disease causing vector to breed. @ hina, rahil class11 cube kanpur. Dated 8.08.21
How we will ensure that Cubists discovered a new MEDIUM for growing soil nematodes?
Please announce, with prominence, if you are confident.
No I would like to wait to see how many days the rava sugar medium for nematodes work.
Any finding of mosquito breeding this season in and near HomeLabs of cubists in CUBE Kanpur?

Please announce. @Hina Mudgal Kanpur CUBE Rahil
Sir, I’m noticing this butterfly for the first time…So actually I wanted to know about it’s local name but no one here has an idea about this butterfly…I haven’t got its local namePothos planters, can we find some MOSQUITO breeding here. Rain water accumulation is a favourite site for MOSQUITO breeding. Prevention is better than cure, avoid collection of water in and around your surroundings. To check out this disease causing vector to breed. @ hina, rahil class11 cube kanpur. Dated 8.08.21
How we will ensure that Cubists discovered a new MEDIUM for growing soil nematodes?
Please announce, with prominence, if you are confident.
No I would like to wait to see how many days the rava sugar medium for nematodes work.
Any finding of mosquito breeding this season in and near HomeLabs of cubists in CUBE Kanpur?

Please announce. @Hina Mudgal Kanpur CUBE Rahil
Sir, I’m noticing this butterfly for the first time…So actually I wanted to know about it’s local name but no one here has an idea about this butterfly…I haven’t got its local name
MOSQUITO prevention and control. MOSQUITO killed by Clap trap . Location outdoor plantation areas. Garden lab. Cll mall pic by rahil cl 11. Dated 8.08.21. @ cube kanpurAre they drowning due to excess water in the cavity I have made on potato slice?@Anshu Kadam (Nematodes) @Aswathy Suresh CUBE @Lakshmy PJ CUBE @Janhvi Deshmukh @Arunan HBSCE
Looks like Aedes@@Hina Mudgal Kanpur CUBE How many Aedes mosquitoes did you find?
Can you see mosquito larvae in this water? If yes any pics
3 mosquito, in the same location …@ batul.
Why? Why?

Why such pretty things are not given names by local population?
Are all butterflies called by the same name? Is it true all parts of India?
Are we still before the time of Enlightenment scientists of Europe?

[KiranKalakotiR (Kiran Kalakoti)]

9th August 2021, 9.30pm to 10th August 2021, 9.30pm
[8/9, 9:23 PM] Arunan HBSCE: Please join in CUBE Chatshaala, if you have not joined today? Interesting discussions on.
[8/9, 10:11 PM] Lydia cUBe Drosphila: Yes it does
Smelling and testing comes under chemical sensation
@⁨Seethalakshmy CUBE⁩ what is chemotaxis and what do you think would be chemical sensation?
[8/9, 10:14 PM] Drishtant Cube Moina: 508th day of CUBE ChatShaala: 9th August 2021
15 CUBists from 15 Centres had joined the webinar
https://www.google.com/maps/d/edit?mid=118h-d48g1_WUcOjIDTSuKXvzN__moWqA&usp=sharing

DELHI
AND College: Aastha Ahuja
Dyal Singh College, Delhi: Dr P. Chitralekha

JHARKHAND
Kokar, Ranchi: Man Masih Beck
Morabadi, Ranchi: Rechel Tirkey

KERALA
Thriprayar: Aswathy Suresh
Pazhuvil, Trissur: Seethalakshmi CR
Thanniam, Thrissur: Sidhy PP
Thrissur: Vinod Kumar K

MAHARASHTRA
Kalyan: Aditya Joshi
Breach Candy, South Mumbai: Arunan MC
Dahanu Road, Palghar: Sachin Pradhan
Bandra, Mumbai: Saida Sayyed
NES Ratnam College, Mumbai: Dr Sunita Chahar

UTTAR PRADESH
Moradabad: Kiran Yadav

WEST BENGAL
Kolkata: Batul Pipewala

Note
CUBist(s) who had joined today but their name isn’t included in the list, kindly reply to this message.
[8/9, 10:25 PM] Jai Kishan CUBE: @⁨Dr Chun Mei Chang⁩
But can’t we alter the levels of metabolites by adding inhibitors or drugs which inhibit the gene regulation required for normal functioning of circadian rhythm?
Will it not be not so difficult even in home lab environment with same experiments, as compared to finding metabolites using HPLC/GC ,etc!
[8/9, 11:52 PM] Seethalakshmy CUBE: Yes, you are right
[8/9, 11:55 PM] Seethalakshmy CUBE: so what is your next plan? Did you want to prove it?
[8/9, 11:58 PM] Seethalakshmy CUBE: I think yes sir
[8/9, 11:59 PM] Seethalakshmy CUBE: Taste (gustation) and smell (olfaction) are called chemical senses because both have sensory receptors that respond to molecules in the food we eat or in the air we breathe. There is a pronounced interaction between our chemical senses. For example, when we describe the flavor of a given food, we are really referring to both gustatory and olfactory properties of the food working in combination.

Reference :

https://courses.lumenlearning.com/waymaker-psychology/chapter/reading-taste-and-smell/
[8/10, 12:13 AM] Seethalakshmy CUBE: To generate the chemotaxis behavior, an animal must recognize a chemical, orient itself in the gradient, and move in a coordinated fashion to the peak concentration of attractant.

The Behavioral Mechanism of Chemotaxis and Thermotaxis

[8/10, 12:14 AM] Seethalakshmy CUBE: @⁨Batul Pipewala Kolkata⁩ ma’am, @⁨Arunan HBSCE⁩ sir, @⁨Lydia cUBe Drosphila⁩, @⁨Aswathy Suresh CUBE⁩
[8/10, 12:30 AM] Seethalakshmy CUBE: Sir, my culture ant is Black crazy ants.
Size :2.3-3mm
Non- clubbed antenna.

the black crazy ant, like other crazy ants, gets its name from its very fast, erratic movement when disturbed. This ant is very hairy and has very long legs and antennae

Reference :
http://piat.org.nz/index.php?page=black-crazy-an
[8/10, 12:33 AM] Seethalakshmy CUBE: Black crazy ants :-

http://idtools.org/id/ants/pia/Fact_Sheets/Paratrechina_longicornis.html
[8/10, 12:56 AM] Seethalakshmy CUBE: This is our Formicarium
Formicarium is used to culture the ants.

Date of setup : 24/5/21
Location : pazhuvil
Time :10:00am

materials required for formicarium

*plastic /glass jar
*Soil and stones
*two bottle caps
(1.Sugar
2. Water)

How do make
*we need plastic /glass jar (here i am using 34cm length jar)
*Put some soil and stones to repeat this process
Now the jar filled
*take two lids of bottles
Take sugar and water and kept on the jar,
Wait for ants.

After these setup i got some ants.

Immediative Objective : To culture the ants

Research question : How do ants communicate.

Now 5th month.
1st - 2nd month they starts to dig soil and starts a new colony
3rd - 4th months i can see they carrying some their own pupae, larvae etc… ( a white thing )
But here a intersting is happens sometimes they carrying the pupae and larvae take out nest. Iam frightened because these thing may be they are shifting their colonies?
As well as some other colonies ant starts to visit their colony my culture ants hated those thing.
Inside the formicarium i can see some paths. I think it have seperate room like thing, because when iam give some sugar they starts to carry inside the nest, and starts to arrange them.
Daily iam observe them that will help to me study it’s behavior.
[8/10, 12:59 AM] Seethalakshmy CUBE: As well as i can see some insects wings and body parts.
An intersting thing sometimes they less active at night. I don’t know what is the reason behind it?
[8/10, 1:06 AM] Seethalakshmy CUBE: They communicate with each other by using their antenna.
I kept some sugar near it,that message will passing through other ant.
When another ant visit their colony they are very disturbed.
What i understand from this they produce various type of chemicals. At particular time.
That is also part of communication.
But which hormone?
[8/10, 1:14 AM] Seethalakshmy CUBE: Where did they produce hormones? Through antenna?
[8/10, 7:42 AM] Batul Pipewala Kolkata:
[8/10, 7:43 AM] Batul Pipewala Kolkata: I have read that ants are scavengers they eat up dead waste so that’s why they could be collecting dead insect parts
[8/10, 8:50 AM] Arunan HBSCE: Any reference to convince others? @⁨Seethalakshmy CUBE⁩
[8/10, 8:51 AM] Arunan HBSCE: What is your doubt about? @⁨Seethalakshmy CUBE⁩ You seen to be not sure.
[8/10, 8:51 AM] Arunan HBSCE: seem*
[8/10, 8:55 AM] Arunan HBSCE: Close up pictures of your ants please! @⁨Seethalakshmy CUBE⁩
How do the male and Queen of Black Crazy Ants look in comparison to workers of the same species? @⁨Seethalakshmy CUBE⁩
[8/10, 8:56 AM] Arunan HBSCE: Which ant species in your locality, closely resemble Black Crazy Ants?
Please give some description so that readers can follow up on ants in their area.@⁨Seethalakshmy CUBE⁩
[8/10, 9:01 AM] Seethalakshmy CUBE: This is a symbiotic relationship between ant and aphid/mealybugs. where ant provide protection from natural enemies and clean the colony and in return scales provide them sugar in the form of honey dew.

Reference :
https://www.researchgate.net/post/Do-ants-feed-upon-aphids-and-mealy-bugs
[8/10, 9:05 AM] Arunan HBSCE: Photo update as evidence please with all essential information like Date, Time, Location, Name of Data collector and CUBE HomeLab. @⁨Seethalakshmy CUBE⁩
[8/10, 9:06 AM] Arunan HBSCE: Don’t you think the date, like 24/5/21 does not match with the description? @⁨Seethalakshmy CUBE⁩
Please clarify.
[8/10, 9:07 AM] Arunan HBSCE: Can’t it be because of daily sleep-wake pattern of these ants, like in Fruitflies or in Phyllanthus plants? @⁨Seethalakshmy CUBE⁩
[8/10, 9:09 AM] Arunan HBSCE: From where did you get the information to understand about chemicals in ants used for communication? @⁨Seethalakshmy CUBE⁩
[8/10, 9:11 AM] Arunan HBSCE: How can hormones help in communication?
*Do you think humans too produce hormones for communication between human beings?* @⁨Seethalakshmy CUBE⁩
[8/10, 9:12 AM] Seethalakshmy CUBE: Ohhh sry sir now 4 th month. Sry for mistake .
Date of setup :24/5/2021.
[8/10, 9:20 AM] Seethalakshmy CUBE: Yes sir! Iam sure, i got a reference to this.
[8/10, 9:24 AM] Arunan HBSCE: Please rewrite the whole with the correction.
Let’s take the responsibility and tell others what is the mistake. Let’s have a culture of recognising and accepting mistakes and correcting. @⁨Seethalakshmy CUBE⁩
[8/10, 9:26 AM] Arunan HBSCE: Please post photo evidences and again post the summary of your Ant model system work at CUBE HomeLab, Pazhuvil. @⁨Seethalakshmy CUBE⁩
[8/10, 9:27 AM] Arunan HBSCE: Please clarify for all to understand with essential details and reference. @⁨Seethalakshmy CUBE⁩
[8/10, 9:28 AM] Seethalakshmy CUBE: Maybe sir! Iam not completly agree with this. ants are sleeping they didn’t take long sleep like phyllanthus i think because each second they are very alert
[8/10, 9:33 AM] Seethalakshmy CUBE: THEY DO - but not in the sense we understand sleep. Research conducted by James and Cottell into sleep patterns of insects (1983) showed that ants have a cyclical pattern of resting periods which each nest as a group observes, lasting around eight minutes in any 12-hour period. Although this means two such rest periods in any 24-hour period, only one of the rest periods bears any resemblance to what we would call sleep. Mandible and antennae activity is at a much lower level (usually up to 65 per cent lower) than during the other rest period in one 24-hour period, indicating a much deeper “resting” phase. Basing and McCluskey in 1986 used brain activity recorders on black, red, and soldier ants to determine whether the deeper resting period constituted actual “sleep”. A steep decline in brain wave fluctuations supported the “sleep” hypothesis in black and red ants, but surprisingly showed a higher level of brain activity in soldier ants in a deep resting phase.

Do ants sleep? | Notes and Queries | guardian.co.uk.
[8/10, 9:37 AM] Seethalakshmy CUBE: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6995257/
[8/10, 9:42 AM] Arunan HBSCE: Reference, please! @⁨Seethalakshmy CUBE⁩
[8/10, 9:44 AM] Arunan HBSCE: What about sleep wake pattern in Fruitflies in comparison with that of ants? @⁨Seethalakshmy CUBE⁩

Seethalakshmi:

This picture i got from this reference. Their colonies show circadian rhythm. Day time they are more active. In night they less active. Not completly inactive, but less active.
[8/10, 9:56 AM] Seethalakshmy CUBE:
[8/10, 9:59 AM] Seethalakshmy CUBE: though the sleeping behaviour of most ants resembles more of a power nap than an eight-hour knock-out. A variety of studies have shown that workers may take anywhere from eight minutes of rest every 12 hours, to over 250 one-minute naps in one day; often at irregular intervals. While the numbers vary considerably, they pale in comparison to the regal sleeping schedule. Queens will often enjoy more regular, longer and deeper sleeping cycles that can last up to nine hours per day — twice the amount of rest a simple worker enjoys.

Do Ants Sleep?

[8/10, 10:01 AM] Seethalakshmy CUBE: On the workers’ side, the power napping behaviour helps ensure “enough worker ants are awake at any one time to protect and serve the colony.”
[8/10, 10:02 AM] Arunan HBSCE: Please post the photos of your ants. @⁨Seethalakshmy CUBE⁩
[8/10, 10:03 AM] Seethalakshmy CUBE: Ohk sir
[8/10, 10:03 AM] Arunan HBSCE: Do you think that resemble the activity Pattern in Fruitflies found by @⁨Aswathy Suresh CUBE⁩ and others?

PDF shared by Seethalakshmi 10.26am
[8/10, 10:26 AM] Seethalakshmy CUBE: The best studied communication behavior in ants is probably chemical communication. Social insects use chemicals to repel predators, and defensive responses are closely connected with alarm communication, and the discharge of alarm pheromones and defensive substances is accompanied by characteristic body movements and postures.

Reference :

Ethological Aspects of Chemical Communication in Ants

[8/10, 10:26 AM] Seethalakshmy CUBE: Ants talk by throwing up on each other to transmit hormones | WIRED UK
[8/10, 10:26 AM] Seethalakshmy CUBE: Ants use pheromones in a number of different ways, such as releasing ‘danger’ pheromones upon death to alert nearby ants, or to create chemical trails from their nest to promising food sources. Other ants in the colony can use their antennas to detect these pheromones and respond accordingly.

https://sciencenode.org/feature/understanding-chemical-communication-in-ant-societies.php
[8/10, 10:29 AM] Seethalakshmy CUBE: Sure sir
[8/10, 10:36 AM] Arunan HBSCE: Histone Deacetylation is studied by Cubists in the case of Moina. Do you think there is an interesting connection between the two? @⁨Seethalakshmy CUBE⁩ @⁨Saida Sayyed⁩
[8/10, 10:37 AM] Arunan HBSCE: Do the ants touch each other’s antenna for communication?

Video shared by Moina Shaikh at 10.59am
[8/10, 10:59 AM] +91 98677 25289: Location : Seawoods Darave (Railway station)
Date : 8 August 2021
Time : 7:10 pm
Video taken by : Moina Latif Shaikh
Insect : Common Green Grasshopper
[8/10, 11:00 AM] +91 98677 25289: Omocestus viridulus - Wikipedia

[8/10, 11:05 AM] Seethalakshmy CUBE: Wowwww! Great pictures.
How can take good pictures like this?

What they are doing? @⁨Moina Shaikh☺️⁩
Which plant is this?
[8/10, 11:07 AM] Janhvi Deshmukh: Yes good pictures of grosshopper @⁨Moina Shaikh​:smiling_face:⁩ they are male female?
[8/10, 11:15 AM] Aditiya Joshi CUBE: Nice pics @⁨Moina Shaikh☺️⁩

Seethalakshmi:

Breaking News

Iam observed 2new plants(red) in my cardamine Culture. This is the first time my plants have followed so many days. 2 plants already i have ( blue).

Total number of plants : 4

Location : Pazhuvil, Thrissur, Cube Home Lab.
Date of photo : 10/8/21.
Time: 11:00 am.
Date of collection : 25/6/2021.
Data collector : Seethalakshmi. C. R. Cube Nattika
[8/10, 11:30 AM] Seethalakshmy CUBE: @⁨Arunan HBSCE⁩ sir,@⁨KIRAN CUBE⁩ ma’am,@⁨Arunima SN Nattika Cardamine⁩ ,@⁨Lydia cUBe Drosphila⁩ ,@⁨Lakshmy PJ CUBE⁩ ,@⁨Shraddha Sonavane CUBE⁩ And others
[8/10, 11:54 AM] +91 98677 25289: Both (one male and one female)
Seethalakshmi:

𝐂𝐚𝐫𝐝𝐚𝐦𝐢𝐧𝐞 𝐌𝐨𝐝𝐞𝐥 𝐬𝐲𝐬𝐭𝐞𝐦
𝑺𝒉𝒐𝒓𝒕 𝒕𝒆𝒓𝒎 𝒐𝒃𝒋𝒆𝒄𝒕𝒊𝒗𝒆: to observe the leaf patter of cardamine( simple to compound leaf) .
𝑳𝒐𝒏𝒈 𝒕𝒆𝒓𝒎 𝒐𝒃𝒋𝒆𝒄𝒕𝒊𝒗𝒆: floral dip method .
Date of photos:7/4/21
Time:6:20.
Date of seed collection : 30/3/21
Time:12:00pm
Location : Pazhuvil,Thrissur,Kerala
Data collector: Seethalakshmi.C.R.cube sn clg Nattika

My previous Data in cardamine Model system.
3rd attempt ( failed😓)

[KiranKalakotiR (Kiran Kalakoti)]

10th August 2021, 9.30pm to 11th August 2021, 9.30pm

9.30 pm Janhvi Deshmukh

[8/10, 9:40 PM] Janhvi Deshmukh: This is my open bait setup in which l see two types of pupae and today I see some water condensation in bait I afraid that when pupae hatched out the flies are died due to water condensation so what can I do that time?
Location:wardha
Date :10/08/2021
Data collector : janhvi deshmukh
Janhvi deshmukh homelab bajaj collage of science wardha Maharashtra
[8/10, 9:41 PM] Man Masih Beck RANCHI CUBE: So you’re having Pupae of houseflies and pupae of fruitflies in the same container. Are they interacting with each other?
[8/10, 9:44 PM] Man Masih Beck RANCHI CUBE: Is your lid detachable?
[8/10, 9:44 PM] Janhvi Deshmukh: No man they not interacting each other
[8/10, 9:44 PM] Janhvi Deshmukh: Yes
[8/10, 9:46 PM] Man Masih Beck RANCHI CUBE: why dont you transfer your lid into some other larger transparent container? like plastic bags or larger plastic box? This way, you can atleast save the pupae attached to the lid.
[8/10, 9:51 PM] Janhvi Deshmukh: Ya I do that but what about other pupae which in side of box? @⁨Man Masih Beck RANCHI CUBE⁩
[8/10, 10:14 PM] Aswathy Suresh CUBE: So, How many culture bottles currently do you have In Prabadhavi Mumbai?
[8/10, 10:17 PM] Aswathy Suresh CUBE: Why 17 days old bottle media became liquidy? Is it effected your flies?
[8/10, 10:24 PM] Aswathy Suresh CUBE: FRUIT FILES COLLABORATORS
Different parts of country.

Aswathy Suresh (me) - Bottukadavu,Thrissur Kerala.
Yash Shergare - Prabadhavi, Mumbai.
Isha Pawle - Worli, Mumbai.
Rechal Tirkey - Morabadi, Ranchi.
Kiran Yadav - Moradabad, Uttar Pradesh.
Janhvi Deshmukh - Wardha, Mumbai
Aastha Ahuja - Faridabad Delhi
Ishita - Jorhat, Assam.

Currect Fruit Flies Collaborators (Who are culturing fruit flies in homelab).
[8/10, 10:30 PM] Aswathy Suresh CUBE: One our Hypothesis is Native (Wild growing) D.melanogaster have better olfaction (Smelling capacity) compared to CsBz. Because CsBz - D.melanogaster started to culture almost 70 years ago (1950). Almost 3360 generation passed. There could a chance that mutant will form (Because of several other reasons).
[8/10, 10:37 PM] Aswathy Suresh CUBE: Great @⁨Yash Sheregare CUBE⁩ .
[8/10, 10:39 PM] Aswathy Suresh CUBE: How many days old culture bottle is this @⁨Yash Sheregare CUBE⁩!? What is your further plan?
[8/10, 10:51 PM] Aswathy Suresh CUBE: Nice Summary . What is your objective? Could you please elaborate?
[8/11, 9:07 AM] Arunan HBSCE: Good! @⁨Aswathy Suresh CUBE⁩

But, @⁨Man Masih Beck RANCHI CUBE⁩ & @⁨Aastha Ahuja Cube⁩ proposed another Hypothesis that Recently trapped local Fruitflies have better capacity for smelling than Lab cultures of local Fruitflies kept in HomeLab for more than one year.
Please comment. @⁨Aswathy Suresh CUBE⁩ @⁨Man Masih Beck RANCHI CUBE⁩ @⁨Aastha Ahuja Cube⁩ @⁨Seethalakshmy CUBE⁩ @⁨Yash Sheregare CUBE⁩
[8/11, 9:23 AM] Batul Pipewala Kolkata: I too agree with this hypothesis @⁨Aswathy Suresh CUBE⁩ can try this experiment and prove or falsify this hypothesis.So what all do you need for such an experiment? What plan would you follow please write details @⁨Aswathy Suresh CUBE⁩
VIDEO OF NEMATODE BY BATUL PIPEWALA at 9.28AM

Original Nematode culture set up with soil on 29/7/21 showing dense growth of nematodes 13days later today 11/8/21 Cube Homelab Kolkata BatulP
video shared by Batul Pipewala at 9.43am
Nematode culture B started on 6/8/21 by transferring nematodes from original culture A shows nematode growth six days later pic 11/8/21 Homelab Kolkata BatulP

[8/11, 9:44 AM] Batul Pipewala Kolkata: The culture had 4drops of milk added to it on first day,there is no soil and everyday two drops of water is added twice.
[8/11, 9:53 AM] Arunan HBSCE: Which medium is this? @⁨Batul Pipewala Kolkata⁩
[8/11, 9:55 AM] Man Masih Beck RANCHI CUBE: After calculating we found that around 48 generations have passed in 1 year old culture bottles of @⁨Aswathy Suresh CUBE⁩ .
Let’s take a Hypothetical situation:
In human terms, 48 generations ago, humans would have been in pre-Christ era 400 BC(taking human age as 50 years on an average). The way people used to think and behave at that time was quite different than the people now.
Suppose some people were trapped and were being maintained in a closed environment since 400 BC.
Then in 2021, their descendants are being compared behaviourly with that of free human descendants. Don’t you think that there would be a difference in the behaviour of isolated humans vs free humans?
@⁨Aswathy Suresh CUBE⁩ @⁨Batul Pipewala Kolkata⁩ @⁨Arunan HBSCE⁩ @⁨Yash Sheregare CUBE⁩
There is a reason why I believe there would be a difference between behaviours…
[8/11, 9:56 AM] Batul Pipewala Kolkata: Raw potato medium
[8/11, 9:58 AM] Batul Pipewala Kolkata:
[8/11, 9:58 AM] Arunan HBSCE: Please describe the " kind of interactions which are normal in nature"?@⁨Batul Pipewala Kolkata⁩
[8/11, 10:00 AM] Arunan HBSCE: Any comments? @⁨Aswathy Suresh CUBE⁩ @⁨Lydia cUBe Drosphila⁩
[8/11, 10:02 AM] Arunan HBSCE: Yes, the same medium & culture doing well in SLBKE & SLBK B?! @⁨Yash Sheregare CUBE⁩ @⁨Aswathy Suresh CUBE⁩
So, what could have gone wrong with SLBK A & SLBK C fly cultures?
[8/11, 10:06 AM] Arunan HBSCE: Please give all essential information like which ants, how old is the set up, what is placed in the two containers and why?
This is especially important since your updates have not been on a daily basis. So it is important to communicate all essential information with each photo. @⁨Seethalakshmy CUBE⁩
[8/11, 10:08 AM] Arunan HBSCE: Do you have a Control photo of the daytime activity? Please always post such Control evidence for people to get convinced. @⁨Seethalakshmy CUBE⁩
[8/11, 10:10 AM] Arunan HBSCE: Please give the essential information like magnification used and what is your guess, etc. @⁨Batul Pipewala Kolkata⁩
[8/11, 10:10 AM] Batul Pipewala Kolkata: Interactions with their natural microbiome consisting of beneficial bacteria,fungi,diatoms,algaearound them,as also mites,snails living in the soil with whom they interact.Their survival and evolution depends on these natural environment which is absent in a lab
[8/11, 10:11 AM] Arunan HBSCE: How about a video of this?
[8/11, 10:13 AM] Batul Pipewala Kolkata: Magnification is 450 times,observation under 45X of compound microscope. Size of the organisms will be in microns.My guess is they.are diatoms or blue green algae
[8/11, 10:14 AM] Batul Pipewala Kolkata: Ok sir
[8/11, 10:16 AM] Arunan HBSCE: Please give essential description with each photo and especially when updates are infrequent when we want others including potential collaborators, to respond.@⁨Saida Sayyed⁩
Please repost with essential details @⁨Saida Sayyed⁩
[8/11, 10:20 AM] Arunan HBSCE: Great! @⁨Aditiya Joshi CUBE⁩ What is your guess on the species that you see more frequently encounter in Kalyan- Murbad Road? @⁨Aditiya Joshi CUBE⁩

Which other plants are growing fast during monsoon and complete the Lifecycle, which we can consider as Monsoon Fast Plants of Kalyan Locality? @⁨Aditiya Joshi CUBE⁩
[8/11, 10:22 AM] Arunan HBSCE: Which species of Phyllanthus are observed in Kalyan Locality in August?
And which percentage of which species?
[8/11, 10:22 AM] Arunan HBSCE: @⁨Aditiya Joshi CUBE⁩
[8/11, 10:22 AM] Janhvi Deshmukh: Great ! @⁨Aditiya Joshi CUBE⁩ so you can which phylluntus species is that?
[8/11, 10:24 AM] Janhvi Deshmukh: We can’t see fruit here ? @⁨Aditiya Joshi CUBE⁩
[8/11, 10:25 AM] Arunan HBSCE: What is your guess of the species?
Can we develop a simple method to identify the Phyllanthus species growing as weeds near city roads? @⁨Aditiya Joshi CUBE⁩
Please develop one and post for others to refine it. @⁨Aditiya Joshi CUBE⁩
[8/11, 10:26 AM] Arunan HBSCE: Any guess which species? @⁨Aditiya Joshi CUBE⁩
[8/11, 10:26 AM] Arunan HBSCE: Which are the other common plants growing along with Phyllanthus species? @⁨Aditiya Joshi CUBE⁩
[8/11, 10:29 AM] Arunan HBSCE: Please give the details of the survey. What percentage of sites surveyed showed Mosquito breeding sites?
What was the design of the survey?
How was it done?
Who were the participants? @⁨Hina Mudgal Kanpur CUBE⁩
[8/11, 10:30 AM] Arunan HBSCE: How frequent is the rain? Which exact locality in Kanpur? @⁨Hina Mudgal Kanpur CUBE⁩
[8/11, 10:33 AM] Arunan HBSCE: What is the percentage of germination?
Please give some more details like when were seeds sown?
What is your objective?
That will help in others joining and taking cues from your success story @⁨Lakshmy PJ CUBE⁩

What do you say? @⁨Seethalakshmy CUBE⁩
[8/11, 10:34 AM] Arunan HBSCE: Percentage of seeds germinated, out of the total seeds sown? @⁨Lakshmy PJ CUBE⁩
[8/11, 10:35 AM] Arunan HBSCE: What is your objective? @⁨Janhvi Deshmukh⁩ When did you set up this trap?
[8/11, 10:37 AM] Arunan HBSCE: How are you sure some pupae are of houseflies? Please explain @⁨Janhvi Deshmukh⁩
[8/11, 10:37 AM] Arunan HBSCE: How do you expect pupae to interact? @⁨Janhvi Deshmukh⁩
[8/11, 10:38 AM] Arunan HBSCE: What is your objective? @⁨Janhvi Deshmukh⁩
[8/11, 10:39 AM] Janhvi Deshmukh: My objective to trapped the fruit fly and see the Lifecycle of fruitfly…I keep this set on 28/07/201 at 7.30 pm
I trapped flies on 29/07/2021 on 8.30pm
Location:wardha
Date :11/08/2021
Data collector: janhvi deshmukh
Janhvi deshmukh homelab bajaj collage of science wardha
[8/11, 10:41 AM] Janhvi Deshmukh: Actually I see some big pupae and some small pupae …big pupae are red in.colour and size is 4 mm and small pupae some.whitish yellow in colour size is 3 mm and the shape also different of big pupae sir …@⁨Arunan HBSCE⁩ sir

Small and Big Pupa @arun

[8/11, 10:44 AM] Janhvi Deshmukh: And also today I seen some housefly hatched …@⁨Arunan HBSCE⁩ sir
[8/11, 10:45 AM] Arunan HBSCE: Interesting! @⁨Man Masih Beck RANCHI CUBE⁩
Looking for comments from all!
Let’s develop this idea, critically.
Are we talking about mutations? @⁨Man Masih Beck RANCHI CUBE⁩
@⁨Yash Sheregare CUBE⁩ @⁨Aswathy Suresh CUBE⁩ @⁨Lakshmy PJ CUBE⁩ @⁨Janhvi Deshmukh⁩ @⁨Seethalakshmy CUBE⁩ @⁨Saida Sayyed⁩ and others
[8/11, 10:47 AM] Arunan HBSCE: Any reference, with respect to Nematodes like C elegans and it’s microbiome?

What exactly do we mean by microbiome? @⁨Batul Pipewala Kolkata⁩
[8/11, 10:48 AM] Arunan HBSCE: In what range of microns? What is your assessment and why? @⁨Batul Pipewala Kolkata⁩
[8/11, 10:50 AM] Arunan HBSCE: When do you expect these flies in your trap to complete the Lifecycle?
@⁨Janhvi Deshmukh⁩
[8/11, 10:51 AM] Janhvi Deshmukh: After 4 or 5 days @⁨Arunan HBSCE⁩ sir
[8/11, 10:51 AM] Arunan HBSCE: Don’t you expect new flies to emerge within these days?
Why no mention of that? @⁨Janhvi Deshmukh⁩
[8/11, 10:53 AM] Janhvi Deshmukh: I expect 1 or 2 flies emerge in 2 days @⁨Arunan HBSCE⁩ sir
[8/11, 10:53 AM] Arunan HBSCE: Photo evidence please! @⁨Janhvi Deshmukh⁩
What happened to Fruitflies?

What is the Lifecycle of Fruitflies in comparison with that of Houseflies?@⁨Janhvi Deshmukh⁩
[8/11, 10:54 AM] Janhvi Deshmukh: I sir today I share some pictures @⁨Arunan HBSCE⁩ sir
[8/11, 10:54 AM] Arunan HBSCE: 4 or 5 days after trapping? What is your reasoning? Please describe your reasoning. @⁨Janhvi Deshmukh⁩
[8/11, 10:55 AM] Arunan HBSCE: On what basis do you have this expectation? @⁨Janhvi Deshmukh⁩
[8/11, 10:55 AM] Janhvi Deshmukh: No sir after trapping on 29/07/2021 I think after 4 or five days I see flies @⁨Arunan HBSCE⁩ sir
[8/11, 10:56 AM] Arunan HBSCE: Let’s not claim without evidence or without a plausible reasoning. @⁨Janhvi Deshmukh⁩ @⁨Aditiya Joshi CUBE⁩ @⁨Saida Sayyed⁩
[8/11, 10:56 AM] Janhvi Deshmukh: Actually I see some pupae chage it’s colour @⁨Arunan HBSCE⁩
[8/11, 10:57 AM] Arunan HBSCE: Do you have photo evidence? @⁨Janhvi Deshmukh⁩ @⁨Aswathy Suresh CUBE⁩ @⁨Yash Sheregare CUBE⁩
[8/11, 10:57 AM] Arunan HBSCE: Any reference to reinforce your argument? @⁨Janhvi Deshmukh⁩ @⁨Seethalakshmy CUBE⁩ @⁨Saida Sayyed⁩
[8/11, 10:57 AM] Janhvi Deshmukh: Yes sir @⁨Arunan HBSCE⁩
[8/11, 11:12 AM] Seethalakshmy CUBE: Great​:+1: @⁨Man Masih Beck RANCHI CUBE⁩
Yes , behavior of both humans are different.
According to this here @⁨Aswathy Suresh CUBE⁩ 1year old culture mutation is possible, but i think less mutation compared to 70 year old culture.
We compare with recently traped local flies with her 1 year old culture mutation will happens
[8/11, 11:24 AM] Seethalakshmy CUBE: Sure sir! I will post
[8/11, 11:25 AM] Aswathy Suresh CUBE: 509th day of CUBE ChatShaala: 10th August 2021

41 CUBists from 40 Centres had joined the webinar

INDIA

DELHI
AND College: Aastha Ahuja
Delhi : Garima Kalakoti
Delhi University : Kamakshi Srivastave
New Delhi:Raj Yashwantrao Patil

JHARKHAND
Kokar, Ranchi: Man Masih Beck
Morabadi, Ranchi: Rechel Tirkey

HARIYANA
Panipat : Aashi

KERALA
Thriprayar: Aswathy Suresh
Kandassankadavu : Lakshmy P J
Pazhuvil, Trissur: Seethalakshmi CR
Thanniam, Thrissur: Sidhy PP

MAHARASHTRA
Breach Candy, South Mumbai: Arunan MC
Worli, Mumbai : Isha Pawle
Wardha : Janhvi Deshmukh
Bandra, Mumbai: Saida Sayyed
NES Ratnam College, Mumbai: Khan Shaheen
CHM College, Ulhasnagar : Sakshi Didla
Prabhadevi, Mumbai : Yash Sheregare

TAMIL NADU
PSGR Krishnammal College Coimbatore : Arunima

UTTARAKHAND
Berinag, Pithoragarh: Darshana Joshi
Uttarakhand : Sakshi

UTTAR PRADESH
Noida:Divyanshi Chauhan
kanpur:Heshica Battina Chowdary
Moradabad: Kiran Yadav
Ghaziabad:Shriya varma
VigyanShaala : Neha Yadav,Aakansha Yadav,Aarti Vankatesan,Sachin Ansari,Poonam Yadav,Kanchan Baflia.

WEST BENGAL
Kolkata: Batul Pipewala

•Diksha
•Dr. K. Mohandas
•Mirza Mubarshra
•Nikhel
•Prachi Pant
•R G
•Snehal
•Teki Sandhyasri
•Visheshta Sharma

Note
CUBist(s) who had joined today but their name isn’t included in the list, kindly replied to this message.

Video shared by Janhvi at 11.27am Data collector: janhvi deshmukh
Janhvi deshmukh homelab bajaj collage of science wardha

See some fruitfly pupae some are yellow in colour and one are brown in colour.

Today i see 3 house fly
[8/11, 11:45 AM] Man Masih Beck RANCHI CUBE: Great Yesterday you had hypothesized that the fruitflies would be emerging out on 11th or 12th August. But instead, housefly emerged out on 11th Aug. What hypothesis can you make after the emergence of houseflies today??
[8/11, 12:17 PM] Aswathy Suresh CUBE: Great @⁨Janhvi Deshmukh⁩. How many old this setup!?
[8/11, 12:17 PM] Janhvi Deshmukh: 10 days old @⁨Aswathy Suresh CUBE⁩
[8/11, 12:18 PM] Janhvi Deshmukh: Yes mam but we want fruitfly so I am waiting for fruitfly now @⁨Man Masih Beck RANCHI CUBE⁩
[8/11, 12:19 PM] Aswathy Suresh CUBE: Did you observed today any newly emerged flies in this setup!?
[8/11, 12:21 PM] Janhvi Deshmukh: No on 8 th August I observed one fly emerge aswathy
[8/11, 12:21 PM] Aswathy Suresh CUBE: What is your further plan!?
[8/11, 12:21 PM] Aswathy Suresh CUBE: So, Today how many flies in there!?
[8/11, 12:22 PM] Janhvi Deshmukh: No. Only housefly seen in the box all fruitfly die …
[8/11, 12:47 PM] Aswathy Suresh CUBE: Ooohh… What is your further step in order to trap only fruit flies not other insects!? Is there any plan on your mind!?

Video shared by Shrudiga at 1.06pm
I’ve observed this buttetfly in my home… Looks beautiful… I think they are just roaming around… But looks beautiful

[8/11, 1:06 PM] +91 97783 22237: Short banded sailer Short banded sailer - Google Search

Its name is Short banded sailer…
[8/11, 1:23 PM] Aswathy Suresh CUBE: Summary : CUBE Home Lab.
Location - Bottukadavu;Thrissur Kerala.

Model System : Fruit Flies.

I Had 4 Single Line Culture in my Place - A, B, C, E

A - Line - 4 Subculture Bottles
SLBK A18, A19, A20, A21

B - Line - 3 Subculture Bottles
SLBK B18, B19, B20

C - Line - 4 Subculture Bottles
SLBK C18, C19, C20, C21

E - Line - 4 Subculture Bottles
SLBK E13, E14, E15, E16.

Date of Subcultures

•28.05.2021 - SLBK A18, B18, C18, E13

•13.06.2021 - SLBK A19, C19, E14

•02.07.2021 - SLBK A20, B19, C20, E15

•19.07.2021 - SLBK A21, B20, C21, E16

Total 15 subculture bottles have.

In this bottles only A21, B19, B20, E15, E16 ; 5 subculture bottles have ~15-20 Flies, ~10-15 Larvae, 10-20 Pupae present.

SAD BREAKING NEWS happened in Last month July was I lost C culture completely I tried to revive them back but i couldn’t save in emergency time (Because I had exams & Other things need to done in last week around August 1st) so I couldn’t done anything for to save these C Line cultures.

Currently I have A - Line, B - Line, E - Line.

A - Line : also in trouble, because three only one fly is alive. Hope can revive that culture also.

B - Line : Reasonable number of Flies, Larvae, Pupae have in B culture.

E - Line : Also have Reasonable number of Flies, Larvae, Pupae have Present in E culture.

All sub culture bottles I can able to see Microworms…

(No worry about these cultures).

Summary By Aswathy Suresh CUBE SN Nattika

Collaborators : @⁨Arunan HBSCE⁩ sir @⁨Yash Sheregare CUBE⁩ @⁨Isha Pawle⁩ @⁨Ishita Assam⁩ @⁨Janhvi Deshmukh⁩ @⁨Rechel Turkey Cube Ranchi Moina⁩ @⁨Aastha Ahuja Cube⁩ @⁨Man Masih Beck RANCHI CUBE⁩ @⁨KIRAN CUBE⁩ @⁨Batul Pipewala Kolkata⁩

video shared by Batul Pipewala at 2.59pm
Nematodes observed using mobile phone and magnifying glass of 10X at Homelab Kolkata BatulP 11/8/21
[8/11, 3:40 PM] Aswathy Suresh CUBE: Iam expecting using this fly I can revive back my A line culture. I still have a hope from this one fly.
In A21 Bottle I can’t able to see more eggs,larvae, pupae in there. Only this one fly is left inside culture.
[8/11, 3:43 PM] Aswathy Suresh CUBE: Media of the culture is Quite good. There is no Liquidy or water condensation can’t able seen yet
[8/11, 3:44 PM] Aastha Ahuja Cube: Are there any larvae or pupa in c culture bottle ?

Can you take a closer look at those bottles ?remember you thought you lost b line on 13 June but you found larvae in it on 24June , is it possible that we can get such thing in C bottles also don’t loose hope search for something in those bottles …

When did you actually loose C line?

Why are we not preparing replicate bottle is it not possible to create replicates ?
[8/11, 3:49 PM] Priti Kanade: To verify Man’s hypothesis
[8/11, 3:49 PM] Priti Kanade: By carrying out olfaction experiment
1 - Ctrl WT
2- Csbz
3- One year old lab culture

With apt replicates for each of them
[8/11, 3:50 PM] Aswathy Suresh CUBE: Actually, I lost C culture on 22.07.2021.
[8/11, 4:00 PM] Aswathy Suresh CUBE: I transferred 2 flies from C18 culture to C21 culture bottle on 19.07.2021.

SLBK C21
Date ways observed ;

•18.07.2021 - Made TRSV media

•19.07.2021 - Transferred 2 flies from C18 (28.05.2021 subculture) to C21(new media bottles).

•20.07.2021 - Media Normal (No water, No liquidly, No Condensation).

•21.07.2021- Started to observed Condensation around bottle wall. 2 Flies were alive.

•22.07.2021 - media bottom became started to present bubbles, 2 Flies where dyed.

This was happened . @⁨Aastha Ahuja Cube⁩
[8/11, 4:02 PM] Aastha Ahuja Cube: What about c 20 ?
[8/11, 4:02 PM] Aastha Ahuja Cube: Were those 2 flies gravid females
[8/11, 4:03 PM] Aswathy Suresh CUBE: Yes, I did made subculture for to save C - Line flies. Because that time I don’t have any C line flies to transfer in new media bottles. I was waiting for to emerging new flies from C18 bottle.
[8/11, 4:22 PM] Aswathy Suresh CUBE: C 20 Culture there was no flies.

SLBK C20
Date ways observation

•01.07.2021 - Made TRSV Media.

•02.07.2021 - Transfer 30 flies from C19 (13.06.2021 subculture) to C20.

•03.07.2021 - Total 5 flies were Dyed, 15 flies alive. Media perfectly ok, there is no water condensation, No water condensation.

04.07.2021 - I didn’t check these bottle.

•06.07.2021 - Observed total (8+5 flies Dyed) flies dyed. 17 alive. Media had no water condensation, solidified conditions.

•07.07.2021 - No observation

•08.07.2021 - Total flies 12 flies alive. Remaining 18 flies were died. 2 small larvae (1st instar) I observed in side culture bottle.

•10.07.2021 - There is no flies completely dead inside culture. But tgere no water condensation, media had no problem.

Expected some eggs, larvae would be there. But after I didn’t get any flies from this C20 culture bottle.
[8/11, 4:23 PM] Aswathy Suresh CUBE: No, One was female & another one was male.
[8/11, 4:45 PM] Lakshmy PJ CUBE: I got it from cardamine plants at home.
Initially I collected cardamine plants from plant nursery
[8/11, 4:50 PM] Lakshmy PJ CUBE: Long term objective -Floral dip method
Short term objective -

1. To observe life cycle
2. observing heteroblasty
   3)Identifying cardamine plants

Seeds collected -25/7/2021
No of seeds on one seed pod-19
Date of sawing Cardamine seeds- -25/7/2021

Date:30/7/2021
Seeds germinated( cotyledonary stage)-13

Date:31/7/2021
Seeds germinated (cotyledonary stage)-15

Date:6/8/2021
Seeds germinated-17
Cotyledonary stage+ one simple leaf arised

Date:10/8/2021
Seeds germinated-17
Cotyledonary stage+ two simple leaf arised

@⁨Seethalakshmy CUBE⁩ @⁨KIRAN CUBE⁩ @⁨Arunan HBSCE⁩
[8/11, 4:52 PM] Lakshmy PJ CUBE: Out of 19 seeds 17 seeds germinated.
Percentage of seed germination - 89%
@⁨Arunan HBSCE⁩ @⁨Seethalakshmy CUBE⁩
[8/11, 4:52 PM] KIRAN CUBE: Beautiful Cardamine, how have you been maintaining Cardamine in your Home Lab as @⁨Seethalakshmy CUBE⁩ is facing some issues in getting good culture of Cardamine.

Please tell us your method of maintaining these beautiful Cardamine
[8/11, 4:53 PM] Arunan HBSCE: Please give a short summary of your work so far so that all can follow. @⁨Janhvi Deshmukh⁩
[8/11, 4:54 PM] KIRAN CUBE: Tell us the whole story from the beginning how you have harvested seeds? What type of seeds did you sow?
[8/11, 4:54 PM] Arunan HBSCE: Please give us a short summary for us to make any comment. @⁨Janhvi Deshmukh⁩

Hina Mudgal:

[8/11, 4:55 PM] KIRAN CUBE: @⁨Lakshmy PJ CUBE⁩ how do we indentify species of Cardamine?.
[8/11, 4:57 PM] KIRAN CUBE: Ohhh are these eggs of mosquito? Which mosquito’s eggs?
[8/11, 4:58 PM] Hina Mudgal Kanpur CUBE: MOSQUITO eggs trapped, in ovitrap day 3.
Set up by vyom gupta class 9.
Objective to identify the stages of lifecycle of MOSQUITO.
Dated - 11.08.21
[8/11, 4:59 PM] Hina Mudgal Kanpur CUBE: We are identifying the type of MOSQUITO @ hina cube kanpur.
[8/11, 5:04 PM] Janhvi Deshmukh: On 28/07/201 at 7.30 pm I kept the setup on my backyard near the dishwashing area on 29/07/201 at 8.30 pm I got 13 flies I close the box with tip after that on 01/08/2021 I see some larve moiving inside the bait on 02/08/2021 I seen some big larve which is 4 mm in size some are 3 mm in size on 4 rth August I seen some pupue some are whitish yellow in colour size 3mm and some are red in colour size 4 mm I seen some 6,7 fruitfly pupae and 10 to 13 housefly pupae on 7 aug I see one fruitfly emerged I think due to water condensation they die today I see some housefly emerge now I am waiting for fruitfly …
Location:wardha
Data collector: janhvi deshmukh
Janhvi deshmukh homelab bajaj collage of science wardha
[8/11, 5:05 PM] Arunan HBSCE: In how many days?
[8/11, 5:10 PM] KIRAN CUBE: Invitation to join today CUBE chatShaala
PLEASE ADD NAME OF PLACE/AFFILIATION ALONG WITH THE NAME WHILE JOINING THE WEBINAR
Here’s the direct link of the CUBE chatShaala webinar: https://webinar.hbcse.tifr.res.in/b/kir-vgc-6jf

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
[8/11, 6:33 PM] KIRAN CUBE: Excellent discussion is going on in CUBE chatShaala around Cardamine which gives us great context to understand Genetic Engineering much better by just handling this weed we can understand how a foreign gene can be incorporated in plants.

Please join😇
Seethalakshmi:

𝗪𝗵𝗮𝘁 𝗛𝗮𝗽𝗽𝗲𝗻𝘀 ???

  𝗘𝘅𝗽𝗲𝗿𝗶𝗺𝗲𝗻𝘁 
 𝐅𝐨𝐫 𝐞𝐱𝐩𝐞𝐫𝐢𝐦𝐞𝐧𝐭𝐚𝐭𝐢𝐨𝐧 

𝐍𝐞𝐜𝐞𝐬𝐬𝐚𝐫𝐲 𝐒𝐮𝐩𝐩𝐥𝐢𝐞𝐬 :
*3 small beakers.(20 ml)

• Milk
• Vinegar
• Curd
  𝐒𝐭𝐞𝐩𝐬 :

After heating the milk, let it cool for 1 hour.
Take 3 small beakers and add 5 ml of milk each small beakers
In all beaker
Add 2.5𝐦𝐥 vinegar to Beaker 𝗔.
Beaker 𝗕 - Only 5𝐦𝐥 milk.
Beaker 𝗖 - add 2.5 𝐦𝐥 Curd to 5 𝐦𝐥 of milk .

*To observe each hour.

• Beaker 𝗔 : 𝑽𝒊𝒏𝒆𝒈𝒂𝒓 (2.5𝐦𝐥) + 𝑴𝒊𝒍𝒌(5𝐦𝐥)
  *Beaker 𝗕 : 𝑶𝒏𝒍𝒚 𝒎𝒊𝒍𝒌(5𝐦𝐥)
• Beaker 𝗖 : 𝑪𝒖𝒓𝒅(2.5𝐦𝐥)+ 𝑴𝒊𝒍𝒌 (5𝐦𝐥).

𝐄𝐱𝐩𝐞𝐜𝐭𝐚𝐭𝐢𝐨𝐧 :
All of them become Curd.
Only Beaker C we get True curd. Others are false Curd.
Location : Pazhuvil, Thrissur, Kerala.
Date of setup : 11/8/21.
Time: 5:20 pm.
Data collector : Seethalakshmi CUBE Nattika.
Seethalakshmi:

Batul Pipewala:

Nematodes growing on rava sugar are smaller in size approx 1mm compared to the ones growing on raw potato approx 2mm size.Cube Homelab Kolkata BatulP
Seethalakshmi:

After 1 hr

Beaker𝗔: (𝒗𝒊𝒏𝒆𝒈𝒂𝒓 +𝑴𝒊𝒍𝒌 )
I can see some precipitate .
Time:6:20 pm
Date of photo: 11/8/2021
Location : Pazhuvil.
Data collector: Seethalakshmi.C.R. CUBE

Batul Pipewala:

New medium prepared as rava sugar medium is developing fungus after 6days.This time to half spoon of rava and 1/4th spoon of sugar which is boiled with half cup water ,two drops of vinegar is also added.Nematodes will be transferred from old rava medium to this fresh medium.11/8/21Cube Homelab Kolkata BatulP

Seethalakshmi:

After 1 hr
Beaker B
Only Milk

After 1 hr
Beaker B
Curd+milk

[8/11, 7:21 PM] Seethalakshmy CUBE: @⁨Arunan HBSCE⁩ sir,@⁨KIRAN CUBE⁩ ma’am,@⁨Batul Pipewala Kolkata⁩ ma’am, @⁨Jai Kishan CUBE⁩ ,@⁨Rechel Turkey Cube Ranchi Moina⁩ ,@⁨Aswathy Suresh CUBE⁩ ,@⁨Janhvi Deshmukh⁩ ,@⁨Aditiya Joshi CUBE⁩ , @⁨Man Masih Beck RANCHI CUBE⁩ And others
[8/11, 8:11 PM] KIRAN CUBE: Somebody ended the CUBE chatshaala by mistake, i m restarting it please join back everyone
[8/11, 8:12 PM] Seethalakshmy CUBE: Ok ma’am
[8/11, 8:13 PM] KIRAN CUBE: Please join back everyone
[8/11, 8:25 PM] Arunan HBSCE: Joined.
[8/11, 8:49 PM] Dr. Sarita kumar Delhi CUBE: Am unable to join🤔
[8/11, 8:49 PM] Janhvi Deshmukh: Mam try to join

[KiranKalakotiR (Kiran Kalakoti)]

11th August 2021, 9.30pm.to 12th August 2021, 9.30pm
Dr. Chun Mei Chang
have you thought of adding boric acid instead? its a better bactericide and fungicide and won’t change the pH of the media.
Seethalakshmi:

𝐂𝐮𝐫𝐝𝐥𝐢𝐧𝐠 𝐨𝐟 𝐌𝐢𝐥𝐤 . 𝗕𝗶𝗼𝗹𝗼𝗴𝗶𝗰𝗮𝗹 vs 𝗖𝗵𝗲𝗺𝗶𝗰𝗮𝗹 processes.
𝗢𝗯𝘀𝗲𝗿𝘃𝗮𝘁𝗶𝗼𝗻 :
After 6hrs
𝗕𝗲𝗮𝗸𝗲𝗿 𝗔(𝐌𝐢𝐥𝐤+𝐕𝐢𝐧𝐞𝐠𝐚𝐫) : precipitation is observed as well as it starts to semi-solid.
𝗕𝗲𝗮𝗸𝗲𝗿 𝗕 : ( 𝐎𝐧𝐥𝐲 𝐌𝐢𝐥𝐤 ) : A yellow colour thin layer is formed on the top. it is liquidy.
𝗕𝗲𝗮𝗸𝗲𝗿 𝗖 ( 𝐂𝐮𝐫𝐝+𝐌𝐢𝐥𝐤 ) : A yellow colour layer is formed. Semi solid.
Location : Pazhuvil .
Date of setup:11/8/21
Time:5:20 Pm
Data collector : Seethalakshmi .C.R. CUBE Nattika .
[8/12, 12:48 AM] Seethalakshmy CUBE: Great ! @⁨Lakshmy PJ CUBE⁩
So days passing,more seeds starts to germinate right?
Why some seeds germinate late?
These all seeds are from a single plant?
[8/12, 12:50 AM] Seethalakshmy CUBE: How many days after sowing did you get this result?@⁨Lakshmy PJ CUBE⁩
[8/12, 12:57 AM] Seethalakshmy CUBE: Yes @⁨KIRAN CUBE⁩ ma’am you are right.
Can you Tell us "𝗦𝘂𝗺𝗺𝗮𝗿𝘆 𝗼𝗳 𝗖𝗮𝗿𝗱𝗮𝗺𝗶𝗻𝗲 𝗶𝗻 𝘆𝗼𝘂𝗿 𝗖𝗨𝗕𝗘 𝗛𝗼𝗺𝗲 𝗟𝗮𝗯 "
That will help to me . To overcome my problems.
[8/12, 9:16 AM] Arunan HBSCE: What is your interpretation? @⁨Seethalakshmy CUBE⁩
[8/12, 10:06 AM] Batul Pipewala Kolkata: will try
[8/12, 10:09 AM] Arunan HBSCE: Are you convinced? @⁨Batul Pipewala Kolkata⁩ can’t see any fungus in this. @⁨Batul Pipewala Kolkata⁩
[8/12, 10:13 AM] Batul Pipewala Kolkata: I have added two drops of vinegar but last time I added vinegar 5ml the nematodes did not survive so if they do not survive this time I will look for an alternative as boric acid.
[8/12, 10:13 AM] Arunan HBSCE: Please share with others what is the approach to identify based on eggs?
Color of eggs? Shape of eggs? Single eggs vs joined together?
Which references are authentic to base on?
Please share with all?
Is Vyom Gupta actively involved? Please encourage your students to post to the CUBE groups. @⁨Hina Mudgal Kanpur CUBE⁩
[8/12, 10:15 AM] Arunan HBSCE: Any idea which are the host plants of this butterfly?
How will we find it? @⁨Shrudhiga⁩
Any clear references?
[8/12, 10:17 AM] Arunan HBSCE: How about designing better traps for Fruitflies?
Please give your idea, based on your experience as well as from the experience of other cubists? @⁨Janhvi Deshmukh⁩
Batul:

Original nematode culture started on 29/7/21 after 14days has begun to show more dead nematodes or immobile nematodes and is smelling awful so I am discarding it.The nematodes have already been transferred to a fresh raw potato slice on 6/8/21 and are doing well
Please clarify this @⁨Seethalakshmy CUBE⁩
Batul:

Dead and immobile nematodes in the original potato culture of 29/7/21Cube homelab Kolkata BatulP 12/8/21
[8/12, 10:21 AM] Arunan HBSCE: How do compare the smelling capacity of recently trapped with flies from 1 year old cultures? @⁨Seethalakshmy CUBE⁩ @⁨Aswathy Suresh CUBE⁩ @⁨Man Masih Beck RANCHI CUBE⁩
What will be the logic & Design of the experiment?
[8/12, 10:25 AM] Arunan HBSCE: Please post videos of all bottles of SLBK A and SLBK C along with SLBK B & SLBK E.

Please help others to see what is in all these bottles. @⁨Aswathy Suresh CUBE⁩
Let’s also know how to take good videos.
[8/12, 10:26 AM] Arunan HBSCE: What is the source of this nematode? Please give the Context.
What is your immediate plan? @⁨Batul Pipewala Kolkata⁩
[8/12, 10:28 AM] Arunan HBSCE: Please post photos to convince others of what you were able to see.
It may so happen, others may see more flies or larvae or pupae.
Please post to convince others. @⁨Aswathy Suresh CUBE⁩ @⁨Yash Sheregare CUBE⁩
[8/12, 10:29 AM] Arunan HBSCE: What is your immediate plan? @⁨Aswathy Suresh CUBE⁩
[8/12, 10:30 AM] Arunan HBSCE: @⁨Aswathy Suresh CUBE⁩ please follow up to convince others including @⁨Aastha Ahuja Cube⁩
[8/12, 10:31 AM] Batul Pipewala Kolkata: The source of nematodes in this slide is from rava sugar medium.
[8/12, 10:31 AM] Arunan HBSCE: What do you really mean by Olfaction Experiment? @⁨Priti Kanade⁩ @⁨Aswathy Suresh CUBE⁩ @⁨Man Masih Beck RANCHI CUBE⁩
Please describe.
[8/12, 10:33 AM] Arunan HBSCE: Please post more pictures of SLBK C for others to get convinced @⁨Aswathy Suresh CUBE⁩
@⁨Aastha Ahuja Cube⁩ What is your comment?

Shraddha:

Please identify. Black hairy Caterpillar observed on Phyllanthus urinaria plant. Date-12/8/21 location-Panvel. data collected by-Shraddha
[8/12, 10:35 AM] Batul Pipewala Kolkata: Why dont you keep the Caterpillar alongwith some phyllanthus leaves to see it grow into an adult ?
[8/12, 10:36 AM] Arunan HBSCE: Bubbles? What bubbles? Please post photos. @⁨Aswathy Suresh CUBE⁩
Let’s analyse collaboratively and understand what was going on in this bottle. @⁨Aastha Ahuja Cube⁩ @⁨Aswathy Suresh CUBE⁩ @⁨Yash Sheregare CUBE⁩
[8/12, 10:39 AM] Arunan HBSCE: Which SLBKC bottle was the last good culture? @⁨Aswathy Suresh CUBE⁩
Please post photos from your archive.
What do you say? @⁨Aastha Ahuja Cube⁩ @⁨Yash Sheregare CUBE⁩ @⁨Man Masih Beck RANCHI CUBE⁩
[8/12, 10:41 AM] Batul Pipewala Kolkata: Microbiome consists of microbes like bacteria and others present inside the organism and in their immediate surroundings.
the wild, C. elegans harbors a characteristic gut microbiome community that is recruited from its surrounding environment (Dirksen et al. … This set is composed of 12 bacteria from 9 different families that represent the core microbiome of C. elegans based on analyses and empirical studies of intestinal colonization.
https://www.g3journal.org/content/10/9/3025#:~:text=In%20the%20wild%2C%20C.,surrounding%20environment%20(Dirksen%20et%20al.&text=This%20set%20is%20composed%20of,empirical%20studies%20of%20intestinal%20colonization.
[8/12, 10:45 AM] Arunan HBSCE: This requires analysis @⁨Aastha Ahuja Cube⁩ @⁨Aswathy Suresh CUBE⁩ @⁨Man Masih Beck RANCHI CUBE⁩ @⁨Yash Sheregare CUBE⁩
Please post photos of this bottles from your archives from 02 .07.2021 @⁨Aswathy Suresh CUBE⁩

Let’s analyse it collaboratively, because how can 30 flies transferred into a medium bottle have no good culture in the form of larvae & pupae? Interesting to understand what was gone wrong? A great opportunity!
@⁨Aswathy Suresh CUBE⁩ @⁨Aastha Ahuja Cube⁩ @⁨Yash Sheregare CUBE⁩
[8/12, 10:46 AM] Arunan HBSCE: Wah, wah! In how many days? @⁨Lakshmy PJ CUBE⁩
[8/12, 10:49 AM] Arunan HBSCE: Which mosquitoes lay single, scattered eggs? Which mosquitoes do lay eggs in groups attached to each other? @⁨Hina Mudgal Kanpur CUBE⁩ @Rahil and others.
Let’s continue discussion to understand that identification can not be difficult. @⁨Hina Mudgal Kanpur CUBE⁩
[8/12, 10:57 AM] +91 22237: Butterflies of Singapore: Life History of the Short Banded Sailor).

This is the reference for its host plant… Its names are not familiar for me
VIDEO SHARED BY SHRUDIGA AT 11.05am
Date-12/8/2021 Time-11.00AM Location - Cube home lab Thathamangalam Data collector- Shrudhiga

VIDEO SHARED BY MAN AT 11.07AM
Astonished by its color dissimilarity in the bottom and the upper region of the plant …And this color varies from dark green to Light reddish-brown…Why does this vary from plant to plant and from region to region specifically the upper and bottom portion…Is my plant not healthy inside the bag
Date-12/8/2021 Time-11.00AM Location - Cube home lab Thathamangalam Data collector- Shrudhiga
Video shared by Man at 11.07am
ReMan’s Lab, Ranchi 12-08-2021 Alert! Alert! Immediate Objective: To save the Brown Hydra at ReMan’s Lab Video: ~1 cm long(head to foot) Brown Hydra at ReMan’s Lab first observed on 8th Aug, 2021 by @Rechel Turkey Cube Ranchi Moina How you can help: As we are left with no moina, suggestions for alternative feed or design to maintain Hydra is needed urgently @Seethalakshmy CUBE @Aswathy Suresh CUBE @Drishtant Cube Moina @Rechel Turkey Cube Ranchi Moina @Abhijith Vinod Cube Kolenchary Drosophila and others…

Aswathy: PHOTO

new subcultures
SLBK A 22 ; SLBK B21 ; SLBK E17

Model system: Fruit Flies
Transfer date : 12.08.2021
Media Date : 11.08.2021

Single Line Cultures in Bottukadavu Thrissur Kerala A, B, E Line Have Today.

•SLBK A22 - Transferred 1 Fly

•SLBK B21 - Transferred 12 Flies

•SLBK E17 - Transferred 14 Flies

Collaborators : @Arunan HBSCE sir @Yash Sheregare CUBE @Isha Pawle @Aastha Ahuja Cube @Janhvi Deshmukh @Rechel Turkey Cube Ranchi Moina

VIDEO SHARED BY ASWATHY AT 11.30AM
SLBK A22 - A Line Culture
Transfer Date : 12.08.2021

Model System : Fruit Flies
Media : TRSV ; 11/08/2021

Transfer Flies : 1 Fly (Look Like Gravid One)

Expectations :
13.08.2021 : 1st Instar Larvae
14.08.2021 : 2nd Instar Larvae
15.08.2021 : 3rd Instar Larvae
16.08.2021 : Pupae (4th Day)
20.08.2021 : Next generation flies (8th Day).

Observation : 1st Day

Media perfectly solidified condition. Fly is happily feeding media, moving.

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
[11:44 AM, 8/12/2021] Aswathy Suresh CUBE: SLBK B21 - B Line Culture
Transfer Date : 12.08.2021

Model System : Fruit Flies
Media : TRSV ; 11/08/2021

Transfer Flies : 12 Flies
Gravid Flies : 5 Flies
Male Flies : 7 Flies

Expectation :
13.08.2021 : ~20 - 1st Instar Larvae
14.08.2021 : ~30 - 1st & 2nd Instar Larvae
15.08.2021 : ~40 - 1st, 2nd & 3rd Instar Larvae
16.08.2021 : ~20 - Pupae + ~20 Larvae (Different stage)
20.08.2021 : ~20 new flies + ~30 - Pupae + ~30 Larvae (Different stage)

Observation : 1st Day

Media is Solidified.Flies are happily moving around. Expecting some eggs.

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur Kerala.
[11:49 AM, 8/12/2021] Aswathy Suresh CUBE: SLBK E17 - E Line Culture
Transfer Date : 12.08.2021

Model System : Fruit Flies
Media : TRSV ; 11/08/2021

Transfer Flies : 14 Flies
Gravid Flies : 6 Flies
Male Flies : 8 Flies

Expectation :
13.08.2021 : ~30 - 1st Instar Larvae
14.08.2021 : ~40 - 1st & 2nd Instar Larvae
15.08.2021 : ~50 - 1st, 2nd & 3rd Instar Larvae
16.08.2021 : ~25 - Pupae + ~30 Larvae (Different stage)
20.08.2021 : ~25 new flies + ~30 - Pupae + ~60 Larvae (Different stage)

Observation : 1st Day

Media is Solidified.Water droplets present on bottle wall.Flies are happily moving around. Expecting some eggs.

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur Kerala.
[11:51 AM, 8/12/2021] Arunan HBSCE: What is the amount of vinegar or acetic acid that prevents microbial growth, according to you? @Batul Pipewala Kolkata
What does references tell on this?

Let’s understand the scientific basis at every step, even when we want to discard the design, in a convincing manner. @Batul Pipewala Kolkata

Let’s show what is different in the practice of Science, than just trial and error resorted to, by others@Batul Pipewala Kolkata
[11:53 AM, 8/12/2021] Arunan HBSCE: I can see some fly larvae there! @Batul Pipewala Kolkata
[12:24 PM, 8/12/2021] Arunan HBSCE: Why do you think these nematodes are dead? They may be immobile! Look, they are not disintegrating! They look very intact!

Please give us reasons @Batul Pipewala Kolkata
[12:26 PM, 8/12/2021] Arunan HBSCE: Always give the context for others to followoe the story.
Are you sure this was Rava Sugar medium or Rava Sugar Vinegar medium? @Batul Pipewala Kolkata
[12:28 PM, 8/12/2021] Arunan HBSCE: Are these caterpillars feeding on Phyllanthus or they just happened to be there by chance? @Shraddha Sonavane CUBE
What are considered to be pests of Phyllanthus plants? Any reference? @Shraddha Sonavane CUBE
[12:38 PM, 8/12/2021] Arunan HBSCE: This should be announced as BREAKING NEWS! @Lakshmy PJ CUBE
[12:41 PM, 8/12/2021] Arunan HBSCE: Why study Lifecycle of this dangerous animal?@Hina Mudgal Kanpur CUBE
Is it not going to be a public health issue? @Hina Mudgal Kanpur CUBE
[12:43 PM, 8/12/2021] Arunan HBSCE: What do you mean by ’ true curd’? @Seethalakshmy CUBE
Any reference? @Seethalakshmy CUBE @Jai Kishan CUBE and others
[12:46 PM, 8/12/2021] Arunan HBSCE: Won’t these be two different species?
On the other hand , if we want to prove that one medium ( rava- sugar) is better than the other medium (potato), what will be the DESIGN of the experiment? @Batul Pipewala Kolkata
[12:46 PM, 8/12/2021] Drishtant Cube Moina: If not Moina, then you should feed other crustaceans such as Copepods, Ostracods that are common in freshwater habitats. I think you should look around for them in the same waterbody from where you came across the Brown Hydra.
What do others say?
[12:50 PM, 8/12/2021] Arunan HBSCE: Please list the names @Shrudhiga along with common names.

Let’s make ourselves familiar through this collaborative exercise. @Shrudhiga
[12:52 PM, 8/12/2021] Arunan HBSCE: What is your objective? What is the context? @Shrudhiga
[12:52 PM, 8/12/2021] Man Masih Beck RANCHI CUBE: yes… copepods are present at my lab
[12:53 PM, 8/12/2021] Arunan HBSCE: Which plant is this according to you? @Shrudhiga
[12:53 PM, 8/12/2021] Arunan HBSCE: Any resemblance to any other plants? @Shrudhiga
[12:55 PM, 8/12/2021] Arunan HBSCE: Excellent! @Man Masih Beck RANCHI CUBE Where did you get the Hydra from? @Man Masih Beck RANCHI CUBE @Rechel Turkey Cube Ranchi Moina
[12:58 PM, 8/12/2021] Arunan HBSCE: Any special care did you take this time while making and transferring the medium, knowing that last time all 4 bottles did not function equally well? @Aswathy Suresh CUBE

Hope, you had a better design this time. Please describe @Aswathy Suresh CUBE @Yash Sheregare CUBE @Lydia cUBe Drosphila @Man Masih Beck RANCHI CUBE @Saida Sayyed
[1:00 PM, 8/12/2021] Arunan HBSCE: Please take photos with good even background @Aswathy Suresh CUBE
[1:02 PM, 8/12/2021] Arunan HBSCE: Is this not a BREAKING NEWS , that you are starting a culture from a single fly, without knowing whether it is male, female. If female, did it get an opportunity to mate and fertilize its eggs? @Aswathy Suresh CUBE @Seethalakshmy CUBE @Yash Sheregare CUBE @KIRAN CUBE and others
[1:03 PM, 8/12/2021] Arunan HBSCE: What is your expectation? @Aswathy Suresh CUBE
[1:05 PM, 8/12/2021] Arunan HBSCE: Let’s hear, from where these Hydra were harvested and when.

What is the story of these Hydra, so far?
[1:05 PM, 8/12/2021] Arunan HBSCE: Please describe @Man Masih Beck RANCHI CUBE @Rechel Turkey Cube Ranchi Moina

(Old post) ReMan’s Lab Ranchi
*Accidental Discovery by @Rechel Turkey Cube Ranchi Moina *
Aug 8, 2021

Sample collection- 24th July, 2021
Location- Pond from Birsa Agricultural University(BAU)'s campus

Using the plankton net lent from @Dr. G C Baskey Cube Ranchi, Dept. of Zoology, DSPMU Ranchi, Rechel and I(Man Masih) collected water samples from the BAU campus. The objective was to discover native Daphnia or Moina species from Ranchi. Although we found many Cyclops and few other crustaceans in the sample, we couldn’t find any moina or daphnia. Since it seemed that the water samples were insignificant for us, Rechel suggested me to discard them which I didn’t due to my laziness. Today, Rechel visited ReMan’s Lab again and after observing the samples she found the Brown Hydra.

Note- For 14 days,(24th July to 7th Aug) the samples remained unnoticed.

Further Plans
We want to develop a design to study Regeneration in Brown Hydra
@Arunan HBSCE @Shalini CUBE Rachi @Dr. G C Baskey Cube Ranchi @Abhijith Vinod Cube Kolenchary Drosophila @Binita Cube
VIDEO SHARED BY SEETHALAKSHMY AT 1.12PM
[1:12 PM, 8/12/2021] Seethalakshmy CUBE: 𝗨𝗽𝗱𝗮𝘁𝗲 𝗼𝗻 𝗔𝗡𝗧 𝗠𝗼𝗱𝗲𝗹 𝗦𝘆𝘀𝘁𝗲𝗺 .

𝗜𝗺𝗺𝗲𝗱𝗶𝗮𝘁𝗶𝘃𝗲 𝗼𝗯𝗷𝗲𝗰𝘁𝗶𝘃𝗲 : 𝗧𝗼 𝗰𝘂𝗹𝘁𝘂𝗿𝗲 𝘁𝗵𝗲 𝗔𝗻𝘁𝘀.

Date of setup :24/5/21
Date of photo:12/8/21
Time:12:51 pm
Location: Pazhuvil ,CUBE Home Lab
Data collector: Seethalakshmi.C.R.
[2:06 PM, 8/12/2021] Ankit Yadav Xms Work Shop Cube Patkar College: How do you culture ants??
[2:19 PM, 8/12/2021] Aswathy Suresh CUBE: Immediate plan : Make new media and transfer remained one fly. That one fly look like gravid one. So, Iam expecting some new progeny’s will get from this gravid fly.

It was SLBK C19 ; 13.06.2021 made subculture bottles had good number of ~40 more flies, ~25 more larvae, ~60 more Pupae shell observed. That was the last Good culture bottle of C - line.
VIDEO SHARED BY ASWATHY AT 2.32PM
[2:32 PM, 8/12/2021] Aswathy Suresh CUBE: SLBK C19 - 13.06.2021
(subculture of C species)

Transfer flies : ~30
Gravid flies : ~15
Male flies : ~15

15 Days old
Conditions

•~+40 adult flies.
•~25 Pupate.
•~30 Dead flies.
•~15 - 3mm sized Larvae.
•Microworms still observed inside culture bottle.

Update by : Aswathy Suresh CUBE SN Nattika.
[2:34 PM, 8/12/2021] Aswathy Suresh CUBE: This was the last good culture of C - Line C19
[2:51 PM, 8/12/2021] Seethalakshmy CUBE: First we set the formicarium.

This is our Formicarium
Formicarium is used to culture the ants.

Date of setup : 24/5/21
Location : pazhuvil
Time :10:00am

materials required for formicarium

*plastic /glass jar
*Soil and stones
*two bottle caps
(1.Sugar
2. Water)

How do make
*we need plastic /glass jar (here i am using 34cm length jar)
*Put some soil and stones to repeat this process
Now the jar filled
*take two lids of bottles
Take sugar and water and kept on the jar,
Wait for ants.

After these setup i got some ants.
[2:57 PM, 8/12/2021] Batul Pipewala Kolkata: What if you collect some pond water it must have some copepods and water fleas you can try feeding the brown hydra with that
[2:59 PM, 8/12/2021] Batul Pipewala Kolkata: How do you know the fly is gravid by looking at it? Do you use your mobile zoom?
[3:03 PM, 8/12/2021] Batul Pipewala Kolkata: Nematodes survived in rava sugar medium without vinegar@@Arunan HBSCE sir
[3:36 PM, 8/12/2021] Arunan HBSCE: Fantastic! Another Goof-up for celebration!

"The objective was to discover native Daphnia or Moina species from Ranchi.
“Although we found many Cyclops and few other crustaceans in the sample, we couldn’t find any moina or daphnia. Since it seemed that the water samples were insignificant for us, Rechel suggested me to discard them which I didn’t due to my laziness.
Today, Rechel visited ReMan’s Lab again and after observing the samples she found the Brown Hydra.”

“Note- For 14 days,(24th July to 7th Aug) the samples remained unnoticed.”

@Man Masih Beck RANCHI CUBE
Please write a separate account, clearly to celebrate your Goof-ups! @Man Masih Beck RANCHI CUBE &@Rechel Turkey Cube Ranchi Moina
[3:42 PM, 8/12/2021] Arunan HBSCE: @Man Masih Beck RANCHI CUBE writes, “Further Plans
We want to develop a design to study Regeneration in Brown Hydra”.
@Man Masih Beck RANCHI CUBE Is not culturing the Hydra your immediate Objective?

Don’t we have a question how the non green hydra survived so long?

Any explanation?
Please post lavish photos of your set up, to inspire others. @Man Masih Beck RANCHI CUBE @Rechel Turkey Cube Ranchi Moina

How about, posting photo of the habitat from where you collected the samples?
Why not tell us how you collected samples.
Exciting Goof-up and chance findings of a treasure! @Man Masih Beck RANCHI CUBE @Rechel Turkey Cube Ranchi Moina @Dr. G C Baskey Cube Ranchi
[3:45 PM, 8/12/2021] Arunan HBSCE: Please post magnified images with a reference ruler for size and to start identifying from today onward. @Seethalakshmy CUBE
Any clear idea which species of worker ants are these?
Please guess and state which ants these are. @Seethalakshmy CUBE @Shrudhiga
[3:48 PM, 8/12/2021] Arunan HBSCE: Don’t you think this foaming is the result of microbial growth?
Do you see this in all bottles made on the same day?
Which bottles have this foaming?
Please post photos of all 4 bottles of made on 19July to see all have this foaming. @Aswathy Suresh CUBE
[3:49 PM, 8/12/2021] Batul Pipewala Kolkata: The rava sugar medium with 5ml vinegar did not help nematode survive,without vinegar growth is v good but the medium developed fungus in 6days.So this time I am using rava sugar with two drops of vinegar to see if it works
[3:51 PM, 8/12/2021] Arunan HBSCE: 30 dead flies after how many days, in this bottle?

And when did you transfer flies from this to the next bottles?
Please give a summary.
[3:52 PM, 8/12/2021] Batul Pipewala Kolkata: Sir many of them were disintegrating and intact ones were immobile they will die too without feeding. Duaer larvae form at a certain stage in which larva undergoes changes
[3:53 PM, 8/12/2021] Arunan HBSCE: Don’t you think waiting for 40 days for transferring to fresh medium is too risky? @Aswathy Suresh CUBE

What will be the ideal time before which we should transfer flies, to fresh culture bottles? @Aswathy Suresh CUBE @Aastha Ahuja Cube @Yash Sheregare CUBE @Sidhy Kerala Drosophila
[3:55 PM, 8/12/2021] Batul Pipewala Kolkata: 15ml vinegar is being used for 100ml medium to prevent fungal growth in TRSV so 1ml or 2 to 3 drops vinegar is good for 10ml medium which I have made for my petri plate.
[3:57 PM, 8/12/2021] Arunan HBSCE: When did you transfer @SLBK C 19 flies to SLBKC 20 bottles? @Aswathy Suresh CUBE
Why did you wait for so long? Any special reason or we don’t have a definite DESIGN yet to MAINTAIN STOCK CULTURE of Fruitflies? @Aswathy Suresh CUBE @Yash Sheregare CUBE @Man Masih Beck RANCHI CUBE @Lydia cUBe Drosphila @KIRAN CUBE
[3:59 PM, 8/12/2021] Arunan HBSCE: Is that not dangerous, by providing opportunities for microbe to have field day? @Batul Pipewala Kolkata
[4:01 PM, 8/12/2021] Arunan HBSCE: What exactly was your design of experiment, to throw out that hypothesis? @Batul Pipewala Kolkata
Did you have sufficient Replicates and Controls before ruling out the possibility?@Batul Pipewala Kolkata
[4:03 PM, 8/12/2021] Arunan HBSCE: Evidence,please! The photo does not substantiate your claim! Rather, all worms and eggs are intact!

Please provide evidence.
"Let’s base all our decisions on OBJECTIVE EVIDENCE*. @Batul Pipewala Kolkata
[4:07 PM, 8/12/2021] Arunan HBSCE: How many replicates? @Batul Pipewala Kolkata What about Controls?
Please show us photo of the set up with which we are ruling out the possibility that nematodes could grow in TRSV medium!

And @Aswathy Suresh CUBE has proved it by chance in her TRSV medium!
[4:09 PM, 8/12/2021] Arunan HBSCE: Please write down all details to falsify the Hypothesis that TRSV medium supports growth of Nematodes.

Janhvi: PhOTO
[4:26 PM, 8/12/2021] Janhvi Deshmukh: I see some fruitfly feeding on bittergourd
[4:26 PM, 8/12/2021] Janhvi Deshmukh: Janhvi deshmukh homelab bajaj collage of science wardha
[4:35 PM, 8/12/2021] Shraddha Sonavane CUBE:

[5:07 PM, 8/12/2021] KIRAN CUBE: INVITATION TO JOIN TODAY’S CUBE CHATSHAALA

Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in CUBE what’s app groups after the session

Webinar Link : https://webinar.hbcse.tifr.res.in/b/kir-vgc-6jf

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
[5:46 PM, 8/12/2021] KIRAN CUBE: Quickly join, today we have kalpana fellows in the CUBE chatShaala.

Currently introducing them to Moina

@Aswathy Suresh CUBE @Lakshmy PJ CUBE @Aastha Ahuja Cube @Ishita Assam and other Cubists
[5:58 PM, 8/12/2021] Sunita Chahar CUBE: Kiran
Our students are not able to join
[5:58 PM, 8/12/2021] Sunita Chahar CUBE: Server error today
[6:02 PM, 8/12/2021] Sunita Chahar CUBE: I too tried,its showing server error
[6:08 PM, 8/12/2021] Batul Pipewala Kolkata: Added Nalini Singh of Cube Ratnam college please share your work on Nematodes here@@Nalini Singh
[6:09 PM, 8/12/2021] Sunita Chahar CUBE: Give some direct link Kiran, students are struggling to join
[6:09 PM, 8/12/2021] KIRAN CUBE: Direct link
https://webinar.hbcse.tifr.res.in/b/kir-vgc-6jf
[6:17 PM, 8/12/2021] Batul Pipewala Kolkata: Added Shahin of Cube Ratnam college please share your work on Nematodes here giving your location and date@@Shahin🍁
[6:18 PM, 8/12/2021] +91 19166: Thank you for joining us maam there are two more members akansha yadav and poonam yadav
[6:18 PM, 8/12/2021] Janhvi Deshmukh: Welcome Shahin please share your experiment with us
[6:18 PM, 8/12/2021] Batul Pipewala Kolkata: Their WhatsApp numbers please
[6:19 PM, 8/12/2021] +919166: +91 03954 akansha yadav
[6:20 PM, 8/12/2021] KIRAN CUBE: Attention!

Owing to unexpected technical issues regarding the regular Big Blue Button Platform where the CUBE Chatshaala is held everyday, we are shifting (temporarily, for today) to

Jitsi.

Jitsi Meet

In case of any issues, please let us know in the group or on personal.
[6:25 PM, 8/12/2021] +91 19166: +912194 poonam yadav
[6:29 PM, 8/12/2021] KIRAN CUBE: Attention!

Owing to unexpected technical issues regarding the regular Big Blue Button Platform where the CUBE Chatshaala is held everyday, we are shifting (temporarily, for today) to

Jitsi.

Jitsi Meet

In case of any issues, please let us know in the group or on personal.
[6:34 PM, 8/12/2021] Lakshmy PJ CUBE: I collected cardamine plants from yellow seed pods.
In one single seed pod there are 19 seeds.
The seeds were yellow coloured.
Its just like sigle line culture like @Aswathy Suresh CUBE. I am trying to make pure line of cardamine plants.
[6:36 PM, 8/12/2021] Lakshmy PJ CUBE: We can go with the method that we followed in Phyllanthus identification.
By looking on to their leaves, stem, leaves, flowers and fruits. @KIRAN CUBE
[6:38 PM, 8/12/2021] Lakshmy PJ CUBE: Within 10 days. @Arunan HBSCE
[6:43 PM, 8/12/2021] Lakshmy PJ CUBE: Shraddha could you send much more clear picture.

Is it clear to you @Lakshmy PJ CUBE
Sunita: PHOTO OF PHYLLANTHUS
[7:17 PM, 8/12/2021] Sunita Chahar CUBE: From LED to natural light
[7:17 PM, 8/12/2021] Sunita Chahar CUBE: 9th August to 12th August
[7:17 PM, 8/12/2021] Sunita Chahar CUBE: 4 days over
[7:18 PM, 8/12/2021] Sunita Chahar CUBE: Not revived
Photo by Sunita Chahar
Hemant:
[7:21 PM, 8/12/2021] +910727: Why this snails seeing increased in maharashtra kolhapur district farm area
[7:21 PM, 8/12/2021] Sunita Chahar CUBE: Plants in natural light
[7:21 PM, 8/12/2021] +910727: Specifically after last rain

[KiranKalakotiR (Kiran Kalakoti)]

12th August 2021, 9.30pm to 13th August 2021. 9.30pm
Seethalakshmi:

[11:46 PM, 8/12/2021] Seethalakshmy CUBE: This is my culture Ant
Size of ant :- 2.8 mm.
Black Ant
Date of photo : 12/8/21.
Time: 6:18 pm.
Location : Pazhuvil.
Data collector : Seethalakshmi
[11:47 PM, 8/12/2021] Seethalakshmy CUBE: @Arunan HBSCE sir,@Vishnupriya CUBE ,@Shrudhiga ,@KIRAN CUBE ma’am,@Man Masih Beck RANCHI CUBE @Lakshmy PJ CUBE ,@Lydia cUBe Drosphila ,@Aswathy Suresh CUBE And others
[11:47 PM, 8/12/2021] Seethalakshmy CUBE: @Batul Pipewala Kolkata ma’am
Video by Sidhy at 11.57pm
Trapped fruit flies in two bottles with banana bait.

Each bottle have 4 or 5 flies.

Date : 12/08/2021

Bait kept in the kitchen at 5:45 PM

Time of trapping: 6:20 PM to 6:30 PM
Fruit flies were already present in the kitchen at the time of keeping the bait.

Location : Thanniam , Thrissur , Kerala

By Sidhy.P.P , CUBE S N College Nattika
Video by seethalakshmi at 12.10am
[12:10 AM, 8/13/2021] Seethalakshmy CUBE: Close up view of Ant .
in this video we can see pure black abdomen Ants as well as black abdomen with white rings Ants

Location : Pazhuvil
Time :12:44 pm.
Date: 12/8/21
Data collector: Seethalakshmi
[12:12 AM, 8/13/2021] Seethalakshmy CUBE: Yes sir , I think these are black crazy ants
[12:17 AM, 8/13/2021] Seethalakshmy CUBE: Size :2.3-3mm
Non- clubbed antenna.

the black crazy ant,
like other crazy ants, gets its name from its very fast, erratic movement when disturbed. This ant is very hairy and has very long legs and antennae

Reference :
http://piat.org.nz/index.php?page=black-crazy-an

http://idtools.org/id/ants/pia/Fact_Sheets/Paratrechina_longicornis.htm
Photo by Seethalakshmi
[12:33 AM, 8/13/2021] Seethalakshmy CUBE: Hermetia illucens

Kingdom: Animalia
Phylum: Arthropoda
Class : Insecta
Order: Diptera
Family : Stratiomyidae
Genus : Hermetia
Species : illucens
Location : Pazhuvil
Time: 2:12pm
Date: 12/8/21

Reference:

Hermetia illucens

[12:36 AM, 8/13/2021] Seethalakshmy CUBE: Video of this insect
[8:47 AM, 8/13/2021] Arunan HBSCE: Fantastic! So, many fruits & vegetables attract them. Can we make a list of what all attract Fruitflies at our homes? Can we first make a list of possible items that attract Fruitflies and then, verify it.
Please start, now. @Janhvi Deshmukh
[8:47 AM, 8/13/2021] Arunan HBSCE: Is it fresh Bittergourd or partially rotten once?
How was it thrown into the bin?

Please always give essential description with Objective, Location, Date, Time, Name of Data Collector and the name of HoneLab with each diagram.
[8:47 AM, 8/13/2021] Arunan HBSCE: What is your plan? Hope, you will follow up to see till the caterpillar turns into a moth or butterfly @Shraddha Sonavane CUBE

What is your DESIGN? @Shraddha Sonavane CUBE
[8:47 AM, 8/13/2021] Arunan HBSCE: Any reference how others identify Cardamine species? @Lakshmy PJ CUBE
[8:47 AM, 8/13/2021] Arunan HBSCE: Always give essential description of Objective, Location, Date, Time and Name of Data collector and the HomeLab with each photo.@Shraddha Sonavane CUBE
[8:47 AM, 8/13/2021] Arunan HBSCE: What is your guess? @Shraddha Sonavane CUBE
[8:47 AM, 8/13/2021] Arunan HBSCE: Always give all essential description of Objective, Location, Date, Time and Name of Data collector and the HomeLab with each photo. so that readers understand your story, each time @Sunita Chahar CUBE
[8:47 AM, 8/13/2021] Arunan HBSCE: Always give all essential description of Objective, Location, Date, Time and Name of Data collector and the HomeLab with each photo. so that readers understand your story, each time @Sunita Chahar CUBE
[8:47 AM, 8/13/2021] Arunan HBSCE: Interesting! @हेमंत जीवन मगदूम
Please tell us more about it? Which crops or plants these snails feed on?
[8:47 AM, 8/13/2021] Arunan HBSCE: Always give all essential description of Location, Date, Time and Name of Data collector and the HomeLab with each photo. so that readers understand your story, each time & respond promptly.@हेमंत जीवन मगदूम
[8:47 AM, 8/13/2021] Arunan HBSCE: When was the last rain. Please give a bit more specific information.
It’s wonderful to see these snails with can be used to study Learning & Memory among other topics. @हेमंत जीवन मगदूम
[8:48 AM, 8/13/2021] Arunan HBSCE: Not clear! What do you mean by " my culture ant"?
What do you mean by Black Ant?

Are all “Black ants” of the same?

Can you make it more clear?

How many different species of " Black ants" are reported?
Which species of Black ants you think you have in your Formicarium? @Seethalakshmy CUBE
Which are the other type of Black ants?
[8:48 AM, 8/13/2021] Arunan HBSCE: Do you think this is a worker ant, male,Queen or anything else. @Seethalakshmy CUBE
[8:48 AM, 8/13/2021] Arunan HBSCE: Congratulations! @Sidhy Kerala Drosophila

This means, you trapped Fruitflies in your kitchen in less than an hour, during evening from 5:45 pm to 6:30 pm!
What is your further plan? @Sidhy Kerala Drosophila
What is your Long term Objective, Short term and Immediate term Objectives? @Sidhy Kerala Drosophila
[8:48 AM, 8/13/2021] Arunan HBSCE: How did you set up the trap? @Sidhy Kerala Drosophila
[8:48 AM, 8/13/2021] Arunan HBSCE: What is your guess? Are all of them of the same species?
Which species, do you think these could be? Why? Any reference! @Seethalakshmy CUBE

Are they all worker ants?
How do you think the quen ant be? @Seethalakshmy CUBE
Any reference?
[8:48 AM, 8/13/2021] Arunan HBSCE: Are “black ants” different from black Crazy Ants?
Which are the similar looking ants one can get confused? @Seethalakshmy CUBE
[8:48 AM, 8/13/2021] Arunan HBSCE: Photo date please! @Aswathy Suresh CUBE
[9:15 AM, 8/13/2021] KIRAN CUBE: 511th day of CUBE chatShaala: 12th August 2021

32 CUBists had joined the webinar

INDIA

1. Kiran Yadav
2. MC Arunan
3. Aswathy Suresh
4. Shramana
5. Kamakshi
6. Kanchan Batila.
7. Aashi
8. Seetha lakshmy
9. Yash Sheregare
10. Prachi pant
    11.HeshicaBattinaChowdary
11. Anjani Kumar Kashyap
12. Darshana
13. Medha Adiga
14. Batul Pipewala
15. Isha Pawale
16. Diksha
17. Saritha Kumar
18. Sachi Bind
19. Man Mansih Beck
20. Lakshmy P J
21. Aastha Ahuja
22. Vaishali Pant
23. Reshita Deore
24. Shraddha Sonavane
25. Rechal Tirkey
26. Aishwarya Menon
27. Tavleen Kaur
28. Atheena
29. Nalini Singh
30. Priyanka Chaudhary
31. Shriya Verma
32. Poonam Yadav

Note:
CUBist(s) who had joined today but their name isn’t included in the list, kindly replied to this message
[9:22 AM, 8/13/2021] Arunan HBSCE: You forgot to give the photo date of this bottle @Aswathy Suresh CUBE
Please tell us what happened to these flies?
When you subculture them? Then what happened? @Aswathy Suresh CUBE
[9:23 AM, 8/13/2021] Arunan HBSCE: How long before the microbial growth spoilt the culture? @Batul Pipewala Kolkata
[9:23 AM, 8/13/2021] Arunan HBSCE: Don’t we have a theoretical prediction, than trial & error that tells us, what concentration of vinegar prevents microbial growth that interferes with Namatode maintenance @Batul Pipewala Kolkata

Can mere Trial & Error be called Scientific methodology?

What is the scientific basis of “two drops of vinegar”?

What exactly vinegar do in cultures? @Batul Pipewala Kolkata
Let’s be very clear about it @Batul Pipewala Kolkata @Aswathy Suresh CUBE @Yash Sheregare CUBE @Lydia cUBe Drosphila @Seethalakshmy CUBE
[9:23 AM, 8/13/2021] Arunan HBSCE: Not clear at all from your photo evidence! @Batul Pipewala Kolkata Please clarify.
[9:23 AM, 8/13/2021] Arunan HBSCE: Please give us the context of this picture and what is your objective? @Seethalakshmy CUBE
[9:23 AM, 8/13/2021] Arunan HBSCE: Which are the other crazy ants? @Seethalakshmy CUBE
Please clarify.
[9:23 AM, 8/13/2021] Arunan HBSCE: Always give all essential description of Objective, Location, Date, Time and Name of Data collector and the HomeLab with each photo. so that readers understand your story, each time & respond promptly.@हेमंत जीवन मगदूम
[9:23 AM, 8/13/2021] Arunan HBSCE: Always give all essential description of Objective, Location, Date, Time and Name of Data collector and the HomeLab with each photo. so that readers understand your story, each time & respond promptly.@Seethalakshmy CUBE
[9:23 AM, 8/13/2021] Arunan HBSCE: Why are curry leaves showing abnormal axillary buds? Please note, in one of them, an ant is interested! @P. Chitralekha Cube JNU @Dr. Sarita kumar Delhi CUBE @Sunita Chahar CUBE @KIRAN CUBE and others. Photo of Curry leaf plant, 6 August, 2021, CUBE HomeLab, Sophia, Bhulabhai Desai Road, South Mumbai by mcarunan
[9:32 AM, 8/13/2021] Lydia cUBe Drosphila: What do you think, why there is a colour variation?
[9:33 AM, 8/13/2021] Lydia cUBe Drosphila: What does this box contain? And how do you know it’s a housefly?
[9:36 AM, 8/13/2021] Lydia cUBe Drosphila: True! Very beautiful!
How did you identify this butterfly as a short banded sailer butterfly??
[9:59 AM, 8/13/2021] Lydia cUBe Drosphila: What could be the reason @Aswathy Suresh CUBE? What do you think? Did you try to figure out what could’ve gone wrong with your culture?
[10:02 AM, 8/13/2021] Lydia cUBe Drosphila: This is a serious situation
You can’t lose any flies that you’ve cultured for so long!
What’s your plan now? How are you planning to revive or get your cultures back again?
[10:03 AM, 8/13/2021] Janhvi Deshmukh: Yes from some days I see fruitfly attractred some fruits some vegetables some dishes …I seen cucumber , Onion,lemon , bittergourd, tomato,banana, Ginger and do a dish like capati ,rice etc …in my homelab.
Location wardha
Date : 13/08/2021
Janhvi deshmukh homelab bajaj collage of science wardha @Arunan HBSCE sir
[10:09 AM, 8/13/2021] Lydia cUBe Drosphila: @Lakshmy PJ CUBE please help in simplifying the term heteroblasty? What is it?
And how will you observe the life cycle of cardamine plants? What are the different stages?
[10:22 AM, 8/13/2021] Sunita Chahar CUBE: Axillary bud proliferation is some mechanism to increase biodiversity.
[10:22 AM, 8/13/2021] Sunita Chahar CUBE: Its a very good observation!!
[11:00 AM, 8/13/2021] Arunan HBSCE: What is your plan to trap and culture Fruitflies? @Janhvi Deshmukh
Please give a detail Design, stepwise.
[11:00 AM, 8/13/2021] Arunan HBSCE: Do you think curry leaf leaflet shows bud at the junction, as shown in the photo? Is that not strange? @Sunita Chahar CUBE
What is the difference between leaflet and leaves? Or, between simple leaf and compound leaf? @Sunita Chahar CUBE
[11:25 AM, 8/13/2021] Aswathy Suresh CUBE: •This time I followed standard protocol of TRSV media.

•In TRSV media while making added smashed tomato pieces.
(last time added tomato paste, it became liquefy very fast)

•I make sure while flies transferring time media bottle have any water condensation in 3 bottles.
[11:27 AM, 8/13/2021] Sidhy Kerala Drosophila: Thank you Sir
Further plan is to make TRSV medium and start single line cultures.

Immediate objective :

Make single line cultures of fruit flies.

Short term objectives :

1.Family level identification.
2. Conduct experiments based on observations and hypothesis.
3. Understand the life span of fruit fly.
4. Find reliable references for the works on fruit flies.

Long term objectives :

1.Genus and Species level identification.
2. Olfactory sensitivity test.

Collaborating with fruit fly model system group.
[11:35 AM, 8/13/2021] Sidhy Kerala Drosophila: Put banana pieces in two bottles . Banana pieces dipped in vinegar. Hope that will prevent fungal growth. Kept the bottles open in the kitchen. When fruit flies entered , closed the bottles using newspaper and rubber band / thread. Put small holes on it.
In one bottle , there is an ant along with the fruit flies.
[11:39 AM, 8/13/2021] Aswathy Suresh CUBE: Expectations :

12.08.2021 :
Transfer 1 Fly

13.08.2021 :
~20 Eggs

14.08.2021 :
~20 Eggs
~20 1st Instar Larvae

15.08.2021 :
~30 Eggs
~20 1st Instar Larvae
~20 2nd Instar Larvae
~20 3rd Instar Larvae.

16.08.2021 :
~20 Eggs
~30 1st Instar Larvae
~20 2nd instar Larvae
~20 3rd Instar Larvae
~20 Pupae.

20.08.2021 :
~30 Eggs
~40 Different Stage Larvae
~30 Pupae
~20 New Flies.
[11:46 AM, 8/13/2021] Yash Sheregare CUBE: @Sidhy Kerala Drosophila
How are you planning to make a single line culture ?
Are you going to transfer trapped fruit fly in the medium that you will make?
@Aswathy Suresh CUBE @Janhvi Deshmukh @Lydia cUBe Drosphila
[11:47 AM, 8/13/2021] Aswathy Suresh CUBE: Yes sir, I made the all bottle media which I made on 18.07.2021 at the same day and at the same time. That time media making I made a change that I added tomato pasted while cooking media for to get Uniform color in all media bottles.
Microbial growth maybe not much possible on 3days old bottle I think sir.
[11:49 AM, 8/13/2021] Yash Sheregare CUBE: @Aswathy Suresh CUBE Let’s expect some visible larvae in 2 days!

If not then what can be done?
Can we mate a male fly from other culture of C or B line with this Gravid Looking like fly?

Will it be then called a SLBk-A/B or A/C culture?

@Lydia cUBe Drosphila @Janhvi Deshmukh @Arunan HBSCE sir @Batul Pipewala Kolkata maam @Isha Pawle @KIRAN CUBE maam
[11:49 AM, 8/13/2021] Aswathy Suresh CUBE: This was happened in all 4 single line culture bottles at that time. But it was observed on 3 rd day of culture after that I didn’t observed these bubble formation.
Now can’t able seen any bubble formation in those media bottles.
VIDEO BY YASH AT 11.54AM
Breaking News - Plenty of pupae on 6th day of Sub culture Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from Locality

SLPbA2 Flies transferred on - 7/08/2021
6th Day Sub culture
13/08/2021
No of flies - 30
No of larvae - 15- 3mm larvae
No of pupae - 35

Location - Prabhadevi, Mumbai
[12:00 PM, 8/13/2021] Aswathy Suresh CUBE: SLBK C19 - subculture bottle on 13th June 2021. After 15 days later (27th June 2021) observed 30 dead flies.
After 21 Days later transferred flies in to C20 bottle (2nd July 2021)
[12:00 PM, 8/13/2021] Yash Sheregare CUBE: Breaking News - 65 Pupae seen on the 6th day of Sub culture in SLPbB2 culture

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from Locality
6th Day Sub culture

Media prepared - 6/08/2021
Fruit flies transferred - 7/08/2021

13/08/2021 (6th Day culture)
No of flies - ~25
No of larvae - - 20
No of pupae - 65 @Aswathy Suresh CUBE @Sidhy Kerala Drosophila @Isha Pawle @Janhvi Deshmukh @Arunan HBSCE sir
[12:04 PM, 8/13/2021] Yash Sheregare CUBE: Breaking News - 130 Pupae on the 6th day of Sub culture in SLPbB2 culture!

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from Locality
6th Day Sub culture
13/08/2021
No of flies - 40
No of larvae - 20-1mm larvae, 10-2mm Larvae - 8 - 3mm larvae
No of pupae - 130 @Aswathy Suresh CUBE @Sidhy Kerala Drosophila @Isha Pawle @Janhvi Deshmukh @Arunan HBSCE sir @Lydia cUBe Drosphila @KIRAN CUBE maam
[12:08 PM, 8/13/2021] Yash Sheregare CUBE: Head Line Update (Alert)
SLPbA2 has 20 fruit flies alive and 30-40 dead fruit flies in culture

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from Locality

Update of 21th day
Location - Prabhadevi, Mumbai
Gravid fruit fly Transferred on - 24/07/2021

13/08/2021
Observation
No of fruit flies - ~ 20
No of larvae - - 0
No of pupae - 20 unhatched pupae
Dead flies - 30-40
[12:09 PM, 8/13/2021] Aswathy Suresh CUBE: How many old this culture? When are you planning to transfer this flies in to new bottle?
[12:10 PM, 8/13/2021] Yash Sheregare CUBE: Correction* - SLPbA1 culture
[12:13 PM, 8/13/2021] Yash Sheregare CUBE: This is 21 day old culture
I transferred approximately 30 fruit flies from SLPbA1 SLPbA2 on 7/08/2021

After that still there were only 5 flies in SLPbA1 culture on 7/08/2021

Today after 6 days there are 20 fruit flies…
@Aswathy Suresh CUBE
I have a SLPbA2 culture having 30 fruit flies today
[12:17 PM, 8/13/2021] Aswathy Suresh CUBE: Dead flies number is more than alive flies inside culture! What could be the reason according to you!? What you think?
[12:18 PM, 8/13/2021] Yash Sheregare CUBE: Headline update
SlPbB1 with media in bad - fungal infection all over the media surface

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from Locality
Update of 21th day
Location - Prabhadevi, Mumbai
Gravid fruit fly Transferred on - 24/07/2021

13/08/2021
Observation
No of fruit flies - ~ 15
No of larvae - - 5
No of pupae - 15 unhatched pupae
Dead flies - 20-30
[12:25 PM, 8/13/2021] Sidhy Kerala Drosophila: I am planning to make three single line cultures by transferring the gravid flies from these bottles to TRSV medium. Will prepare the medium today and transfer the flies tomorrow.

PHOTO BY YASH
I think because the media now is liquidy and flies have less place to walk and even feed,
they died due to liquid part of the medium…
They are seen at the bottom of the medium on the surface…
Here is a picture of the SLPbA1 media showing bottom surface!
FRUIT FLY CULTURE VIDOES BY ASWATHY AND YASH
[12:30 PM, 8/13/2021] Yash Sheregare CUBE: Headline update
SlPbC1 still a healthy culture having larvae, pupae and adult fruit flies with less dead fruit flies.

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from Locality
Update of 21th day
Location - Prabhadevi, Mumbai
Gravid fruit fly Transferred on - 24/07/2021

13/08/2021
Observation
No of fruit flies - ~ 20
No of larvae - - 30
No of pupae - 50 unhatched pupae
Dead flies - 10-15
[1:05 PM, 8/13/2021] Aswathy Suresh CUBE: Today 1st Day after transfer 1 fly, expected some eggs on bottle wall side but can’t able see any Eggs inside SLBK A22 new subculture on 12.08.2021. Water droplets present down side of culture. Fly is alive inside culture bottle.

Objective : To find D.melanogaster in my native area.

Update : 1st Day (13/08/2021)
Location : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
[1:21 PM, 8/13/2021] Aswathy Suresh CUBE: 1st Day : SLBK B21
B - Line subculture

Update : 13/08/2021;1:16pm
Location : Bottukadavu - Thrissur Kerala.

Transferred Date : 12.08.2021
Transferred Flies : ~12
Gravid Flies : 5
Male Flies : 7
Dead Flies : 3

Objective : Find D. melanogaster from native single line cultures.

Expectation : 1st Day
~30 Eggs.

Observation : 1st Day
0 Eggs
Media Well Solidified
Water droplets present in bottle wall side. Observed today.

Update : Aswathy Suresh CUBE SN Nattika.
[1:24 PM, 8/13/2021] Lydia cUBe Drosphila: This bottle looks so good @Yash Sheregare CUBE! Satisfying!
What’s your plan with this bottle? Did you start with any identification?
[1:27 PM, 8/13/2021] Aswathy Suresh CUBE: 1st Day : SLBK E17
E - Line subculture

Update : 13/08/2021;1:23pm
Location : Bottukadavu - Thrissur Kerala.

Transferred Date : 12.08.2021
Transferred Flies : ~14
Gravid Flies : 6
Male Flies : 8

Objective : Find D. melanogaster from native single line cultures.

Expectation : 1st Day
~50 Eggs.

Observation : 1st Day
0 Eggs
Media Well Solidified
Observed water droplets present in bottle wall side.
No Dead Flies.

Update : Aswathy Suresh CUBE SN Nattika.
[1:31 PM, 8/13/2021] Lydia cUBe Drosphila: What could be the reason for so many dead flies? How old is this bottle?
As per the details provided here,
You transferred one gravid fly on 24/7/2021. Today is 13/8/21. It’s not even a month! So I don’t think the flies are too old to die. What do you think?
[1:36 PM, 8/13/2021] Janhvi Deshmukh: No sir it’s not a rotten bittergourd it’s a dry bittergourd which is use for making dish @Arunan HBSCE sir
[1:38 PM, 8/13/2021] Janhvi Deshmukh: 130 pupae on 6 th day wow what is your next plan @Yash Sheregare CUBE
VIDEO SHARED BY BATUL AT 2.12PM
Nematode culture growing on raw potato slice without soil started on 6/8/21 seven days have passed today13/8/21Homelab Kolkata Batul P
[2:13 PM, 8/13/2021] Batul Pipewala Kolkata: why do you think @Aswathy Suresh CUBE bottles do not show such high number of progeny?
[3:08 PM, 8/13/2021] Aswathy Suresh CUBE: Wow…. Such A Good Healthy Culture.
[3:10 PM, 8/13/2021] Aswathy Suresh CUBE: How Many Flies Did You Transferred In B2 Bottle? How many of them are gravid flies?
[4:39 PM, 8/13/2021] Arunan HBSCE: Do you think 5:30 pm to 6:00 pm should be a good time when Fruitflies look around for food and mate? @Sidhy Kerala Drosophila
[4:39 PM, 8/13/2021] Arunan HBSCE: What is the size range of Black Crazy Ants, according to references? @Seethalakshmy CUBE
[4:39 PM, 8/13/2021] Arunan HBSCE: Interesting close up! @Seethalakshmy CUBE What is special about this?
[4:39 PM, 8/13/2021] Arunan HBSCE: Looks like, you have a new method of preparing bait! @Sidhy Kerala Drosophila
Why did you choose to ip banana in vinegar?
Do you think Vinegar by itself is an attractant for Fruitflies? @Sidhy Kerala Drosophila
Any reference?
What is the size of the bottle? Looks like it is a very small bottle.
Did you choose it deliberately? @Sidhy Kerala Drosophila
[4:39 PM, 8/13/2021] Arunan HBSCE: Interesting idea! @Yash Sheregare CUBE
Please explain so that all others will understand your design.
[4:39 PM, 8/13/2021] Arunan HBSCE: Wah,wah! @Yash Sheregare CUBE @Aswathy Suresh CUBE @Aastha Ahuja Cube @Sidhy Kerala Drosophila @Rechel Turkey Cube Ranchi Moina @Man Masih Beck RANCHI CUBE and others to comment!
[4:39 PM, 8/13/2021] Arunan HBSCE: But, in 6 to 7 days, we should be expecting several pupae and before that several larvae!
Did you miss observing this on 3&4 days and on 6&7 days? @Aswathy Suresh CUBE
What is your comment? @Yash Sheregare CUBE
[4:39 PM, 8/13/2021] Arunan HBSCE: How many flies were transferred to SLBKC 20 bottle on 2 July? @Aswathy Suresh CUBE
Was there larvae and pupae in SLBK 19 bottle on 2 July 2021?
@Aswathy Suresh CUBE @Yash Sheregare CUBE please comment.
[4:39 PM, 8/13/2021] Arunan HBSCE: Are you sure, on 6th day of transfer of Single Gravid female, you got 25 flies on the 6th day? @Yash Sheregare CUBE
[4:47 PM, 8/13/2021] Arunan HBSCE: How many flies transferred to SLPbB2 and when? @Yash Sheregare CUBE I am confused!
[4:49 PM, 8/13/2021] Arunan HBSCE: Yes, @Yash Sheregare CUBE How many day old culture is this?
How many Fruitflies transferred to this bottle?
[5:01 PM, 8/13/2021] Arunan HBSCE: Are you sure, @Yash Sheregare CUBE you transferred one single Gravid Female in SlPbB1 on 24/07/2021?
And today, after 21 days and after transferring flies to SlPbB2, still you have larvae, pupae and adults in the Gravid female bottle. @Yash Sheregare CUBE

Please clarify.
[5:04 PM, 8/13/2021] KIRAN CUBE: INVITATION TO JOIN TODAY’S CUBE CHATSHAALA

Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in CUBE what’s app groups after the session

Webinar Link : https://webinar.hbcse.tifr.res.in/b/kir-vgc-6jf

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
[5:37 PM, 8/13/2021] KIRAN CUBE: Attention!

Owing to unexpected technical issues regarding the regular Big Blue Button Platform where the CUBE Chatshaala is held everyday, we are shifting (temporarily, for today) to

Jitsi.

Jitsi Meet

In case of any issues, please let us know in the group or on personal.
[6:51 PM, 8/13/2021] Seethalakshmy CUBE: Culture Ant which means - The ants that I culture.
Black Ant means - The ant which have black colour body.
Black Crazy ant - The black ant it moves crazily.
[6:56 PM, 8/13/2021] Seethalakshmy CUBE: Many types of back ants have : - Garden ant, Carpenter Ant etc…
CARDAMINE PHOTO BY Lakshmy AT 6.56PM
[6:56 PM, 8/13/2021] Lakshmy PJ CUBE: Date:12/8/2021
Time:5:42pm
Place: Mampilly
Cotyledonary stage+ 3 leaf stage
[6:59 PM, 8/13/2021] Seethalakshmy CUBE: Black garden ant - Wikipedia

This a type of black Ant
(Lasius niger), also known as the common black ant, is a formicine ant.
[7:00 PM, 8/13/2021] Seethalakshmy CUBE: Sir, in my formicarium I have Black crazy ant.
[7:04 PM, 8/13/2021] Seethalakshmy CUBE: This is a worker ant.
In crazy ant colony:
Size of workers: 2. 3 - 3mm

https://entnemdept.ufl.edu/creatures/urban/ants/crazy_ant.htm#:~:text=The%20crazy%20ant%20worker%20is,(hair-like%20projections).
CARDAMINE PHOTO BY Lakshmy AT 7.09PM
Date:12/8/2021
Time:5:42pm
Place: Mampilly
Cotyledonary stage+ 3 leaf stage
[7:11 PM, 8/13/2021] Seethalakshmy CUBE: Yes sir, the crazy ants quite different from common black ants.

Yes sir, at first sight we can feel both them are same. But looking their movement we can easily identify the crazy ant.
[7:18 PM, 8/13/2021] Seethalakshmy CUBE: Yes sir, these are same species I think, i want to check this because
may be some ants Abdomen is white bands they eat some liquid type food.
[7:25 PM, 8/13/2021] Seethalakshmy CUBE: So i want to prove this, so my next plan is to give sugar syrup to all of them, and observe them
[7:28 PM, 8/13/2021] Seethalakshmy CUBE: Two types of crazy ants

• Black crazy Ants
  *Yellow crazy Ants
  [7:31 PM, 8/13/2021] Seethalakshmy CUBE: Hermetia illucens

Kingdom: Animalia
Phylum: Arthropoda
Class : Insecta
Order: Diptera
Family : Stratiomyidae
Genus : Hermetia
Species : illucens

Reference:

Hermetia illucens

Location : Pazhuvil
Time :2:12pm
Date:12/8/21
Data collector : Seethalakshmi. C. R.
[7:34 PM, 8/13/2021] Seethalakshmy CUBE: 2.3-3 mm
[7:36 PM, 8/13/2021] Seethalakshmy CUBE: Iam Wondering about it’s Eyes as well as body shining.
APHID PHOTO BY Janhvi AT 7.48PM:
It’s a aphids ? Location: wardha. Date :13/08/2021. Time :7.48 pm. Data collector: janhvi deshmukh homelab bajaj collage of science wardha

PHOTO AND VIDEO OF MOSQUITO BY HINA AT 8.27PM
MOSQUITO SURVEILLANCE by cube kanpur. Pic by athrv dixit class 10. Spotted MOSQUITO larvae in plant pots. Location- Gumti road.kanpur. dated 13.08.21 Observation- rain water accumulation is breeding sites for this vector. @ hina allen cube
[8:39 PM, 8/13/2021] Arunan HBSCE: Jitsi Meet

Please join back. Very important to mentor the Kalpana fellows.
[9:24 PM, 8/13/2021] Batul Pipewala Kolkata: Is Alzheimer's Hereditary / Genetic? | Alzheimer's Association.
[9:26 PM, 8/13/2021] Batul Pipewala Kolkata: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876682/

[KiranKalakotiR (Kiran Kalakoti)]

13th August 2021. 9.30pm to 14th August 2021, 9.30pm
[9:55 PM, 8/13/2021] Seethalakshmy CUBE: Crazy Ants
[10:04 PM, 8/13/2021] Arunan HBSCE: @Abhijeet Singh Your contribution today, was exemplary! Please continue coordinating with Saida, Kiran & Aswathy to post the map and also the summary of today’s Chatshaala.

If I remember correctly, you joined CUBE in your 8th standard and is back with us, now. Do keep it up!
[10:11 PM, 8/13/2021] Abhijeet Singh: 512th day of CUBE chatShaala: 13th August 2021
INDIA

21 CUBists had joined the webinar

1. Saida Sayyed
2. MC Arunan sir
3. Ashwathy Suresh
4. Kamakshi
5. Aashu
6. Lakshmy PJ
7. Heshica battina Chowdary
8. Sare kamat
9. Prachi pant
10. Aarti Venkatesan
11. Sachin pradhan
12. Shraddha Sonavane
13. Scahi bind
14. Batul Ma’am
15. Seethalakshmi
16. Shramana
17. Viashali pant
18. Khushi Goel
19. Sarita kumar
20. Rishita Deore
21. Abhijeet Singh

Note:
CUBist(s) who had joined today but their name isn’t included in the list, kindly replied to this message
[11:16 PM, 8/13/2021] Saida Sayyed: 512th day of CUBE chatShaala: 13th August 2021
21 CUBists from 19 CUBE centres had joined the webinar had joined the webinar

INDIA

Delhi
Abhishek Singh
Dr Sarita Kumar: ANDC, Delhi
Kamakshi Srivastave : Delhi University
Kanishka Parashar: Jamia Millia Islamia

Gujarat
Rishita Deore

Kerala
Aswathy Suresh: Thriprayar
Lakshmy PJ: Kandassankadavu
Seethalakshmi CR: Pazhuvil, Trissur

Maharashtra
Anshu Kadam: Thane
Arunan MC: Breach Candy, South Mumbai
Sachin Pradhan: Dahanu Road, Palghar
Saida Sayyed: Bandra, Mumbai
Shraddha Sonavane: Panvel

Uttar Pradesh
Heshica Battina Chowdary: kanpur

West Bengal
​​Batul Pipewala: Kolkata
Shramana: Kolkata

Aarti Venkatesan
Khushi Goel
Prachi Pant
Sare kamat
Scahi bind
Viashali Pant

Note:
CUBist(s) who had joined today but their name isn’t included in the list, kindly replied to this message
WHITEBOARD PHOTOS SHARED BY SAIDA AT 12.49AM
[7:29 AM, 8/14/2021] Abhijeet Singh: So @Seethalakshmy CUBE if I(or anybody who doesn’t know what are black ants and black crazy ants) look into your culture and want to distinguish between the common black ants and crazy black ants what peculiar character should we look for?
Which character would easiest to differentiate between the two!?
[8:28 AM, 8/14/2021] Abhijeet Singh: Yes sir!!
I’m going the paper and trying to understand what are the characters of climbing plants they are taking as inspiration to development robots for their purposes.

Going through the paper they say that
However clear and useful biological investigations require a methodological approach to really understand the possible reasons for a particular phenomenon in order to facilitate and correctly translate the working principles from nature to the artificial world

The review is divided into 3 parts i.e. 1) Adaptation. 2) Movement. 3) Behaviour.

ADAPTATION
This section includes the details the current *morphological( form and structure) and biomechanical(structure, function and motion of the mechanical aspects of biological systems) tools and methods used to Investigate the material structure-properties in climbing plants, as well as their tissue architecture and variation in material properties from macro to micro-scale.

MOVEMENT
Includes exploring the recent studies on movements and with and overview of the tracking methods and some software for analysing the kinematics.

BEHAVIOUR
Analysing the approaches used to explore the theories behind the behaviour in climbing plants*

The beauty and efficiency of nature have long Inspired engineers aiming at transferring ideas from biology to technology
The specific term biomimetics ( mimicking biological processes) was coined by bio-engineer Otto Schmitt.

I need to go through it and also see for other references available.
PHOTOS BY ABHIJEET SINGH AT 8.36AM
Very interesting picture😃! As if the plant is using hands to take support for growing!. Ulwe, Navi-Mumbai. 14th August 2021.
Mom tied a rope so that they can grow by taking support. And it seems like it is doing good for them. Ulwe, Navi-Mumbai, 14th August 2021
[9:09 AM, 8/14/2021] Batul Pipewala Kolkata:
[11:52 AM, 8/14/2021] Arunan HBSCE: Good, elementary query @Abhijeet Singh
I am sure @Seethalakshmy CUBE will now attempt to give us some cue.
Please @Seethalakshmy CUBE
[12:17 PM, 8/14/2021] Arunan HBSCE: Please give essential description of the context along with nae of the Data collector & Date along with K cation of the CUBE HomeLab @Abhijeet Singh
[12:17 PM, 8/14/2021] Arunan HBSCE: Some comments please! @Batul Pipewala Kolkata
[12:18 PM, 8/14/2021] Arunan HBSCE: Do you think this is an Insect? Why or why not? @Janhvi Deshmukh
[12:21 PM, 8/14/2021] Arunan HBSCE: Please describe this photo urgently @Hina Mudgal Kanpur CUBE
What is seen like frog eggs?

Always give the essential description to start a discussion. @Hina Mudgal Kanpur CUBE
[12:56 PM, 8/14/2021] Aswathy Suresh CUBE: 25-30days in between Those flies were died completely inside the bottle. Before that I already transferred progeny’s in to new subculture bottle C20
[12:57 PM, 8/14/2021] Janhvi Deshmukh: Yes I think it’s a insect I see some aphids on same plant …so thats why I have a question it’s a aphids??
VIDEO BY SIDHY AT 12.58PM
Fruit flies on Hibiscus flower parts. Date: 14/08/2021 Time: 11:58 AM. Location: Thanniam , Thrissur, Kerala. Data collector : Sidhy.P.P , CUBE S N College, Nattika
[1:00 PM, 8/14/2021] Janhvi Deshmukh: wow !what they do in hibiscus such a beautiful video …what do you think what fruitfly do on hibiscus?
[1:00 PM, 8/14/2021] Janhvi Deshmukh: @Sidhy Kerala Drosophila
[1:00 PM, 8/14/2021] Aswathy Suresh CUBE: Vinegar mainly using for to prevent the fungal contamination because it contains acetic acid and culture we can maintain for quite longer, maybe one month or more than one month in good condition.
[1:01 PM, 8/14/2021] Aswathy Suresh CUBE: Great work @Abhijeet Singh
[1:04 PM, 8/14/2021] Aswathy Suresh CUBE: Ma’am, It could be some other reason I guess . The all flies died with in 10 days. And progeny’s didn’t get it from those flies. It could be the reason those C line flies are old flies maybe.
[1:08 PM, 8/14/2021] Sidhy Kerala Drosophila: I think they can feed on these soft and fleshy mucilage containing parts of hibiscus flower.
[1:09 PM, 8/14/2021] Janhvi Deshmukh: Hibiscus feed on flowers??? @Sidhy Kerala Drosophila @Aswathy Suresh CUBE @Arunan HBSCE sir @Batul Pipewala Kolkata mam and others
[1:10 PM, 8/14/2021] Aswathy Suresh CUBE: My plan is to save remain culture SLBK A, B, E with out loosing any flies. I think I need to make more subculture in one Line.
VIDEO BY SIDHY AT 1.16PM
Trapped fruit flies using Hibiscus flower parts as a bait.Five flies are trapped. Date: 14/08/2021 Time: 12:45 PM Location : Thanniam , Thrissur , Kerala. By Sidhy.P.P , CUBE S N College,Nattika.
[1:16 PM, 8/14/2021] Janhvi Deshmukh: Ohh great ! So how many flies are there ?
[1:16 PM, 8/14/2021] Janhvi Deshmukh: @Sidhy Kerala Drosophila
[1:17 PM, 8/14/2021] Sidhy Kerala Drosophila: There are 5 flies
[1:17 PM, 8/14/2021] Janhvi Deshmukh: Oh so you see any gravid fruitfly? @Sidhy Kerala Drosophila
[1:18 PM, 8/14/2021] Sidhy Kerala Drosophila: Yes. There is one gravid fly.
[1:19 PM, 8/14/2021] Janhvi Deshmukh: Ohh great ! So 4 are male and female
[1:19 PM, 8/14/2021] Janhvi Deshmukh: @Sidhy Kerala Drosophila
[1:19 PM, 8/14/2021] Janhvi Deshmukh: So now what is your objective?@@Sidhy Kerala Drosophila
[1:20 PM, 8/14/2021] Aswathy Suresh CUBE: What is the context discussing about imaginal disc? @Saida Sayyed @Yash Sheregare CUBE @Lydia cUBe Drosphila mam @Batul Pipewala Kolkata mam
[1:21 PM, 8/14/2021] Aswathy Suresh CUBE: Aedes mosquito how many day want to complete their life cycle? @Hina Mudgal Kanpur CUBE mam @Seethalakshmy CUBE @Dr. Sarita kumar Delhi CUBE mam
[1:27 PM, 8/14/2021] Sidhy Kerala Drosophila: Objective is to know whether we can see fruit fly life cycle in this set up using Hibiscus flower.
[1:27 PM, 8/14/2021] Batul Pipewala Kolkata: @Kamakshi of kalpana fellows wants to study the role of imaginal discs in Alzheimers that was the context @Aswathy Suresh CUBE
[1:29 PM, 8/14/2021] Janhvi Deshmukh: Ohh yes mam
[1:29 PM, 8/14/2021] Aswathy Suresh CUBE: Great . What is the relation between imaginal disc & Alzheimer’s study ? Is there any correlation?
[1:30 PM, 8/14/2021] Sidhy Kerala Drosophila: I am a student
[1:30 PM, 8/14/2021] Janhvi Deshmukh: Yes
[1:35 PM, 8/14/2021] Batul Pipewala Kolkata: Summary by kamakshi
Hello everyone,

Today (13th August 2021) in cube chatshaala, we started the discussion with imaginal disc present in drosophila (fruit flies). The discussion started from imaginal disc lead us to understand the relationship of development of flies to their different stages during life cycle. We also understood the concept of imaginal disc, it’s contribution in growth and in which other organisms it is found during development, that are insects. Insects of different economic value and pathogenic activity were discussed including mosquitoes.
Relationship of imaginal discs and brain activity was understood with the help of previously available data on public resources. Also design of the methodology was discussed to understand if there is…
[2:36 PM, 8/14/2021] Dr. Sarita kumar Delhi CUBE: Duration varies according to the environmental conditions…It can be over in 10-12 days and can extend to months.
[2:55 PM, 8/14/2021] DP HBCSE: We will continue our barefoot chat live session-31, this afternoon from 3-4 pm at https://webinar.hbcse.tifr.res.in/b/dp6-vs2-a2s

More Details: BFC Session-31 [August 14 2021] Indian Philsophical Schools - #2 by karnamdpdurga
[5:11 PM, 8/14/2021] KIRAN CUBE: INVITATION TO JOIN TODAY’S CUBE CHATSHAALA

Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in CUBE what’s app groups after the session

Webinar Link :

Jitsi Meet

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
[5:20 PM, 8/14/2021] Arunan HBSCE: What is the defining features of insects? @Janhvi Deshmukh
[5:21 PM, 8/14/2021] Arunan HBSCE: Where exactly did you keep this hibiscus flower parts? @Sidhy Kerala Drosophila
[5:24 PM, 8/14/2021] Arunan HBSCE: Great! @Sidhy Kerala Drosophila Let’s discuss this amazing finding today.
Please join in CUBE Chatshaala.

PHOTO OF CARDAMINE BY LAKSHMY SHARED AT 8.06PM
Long term objective -Floral dip method
Short term objective -

1. To observe life cycle
2. observing heteroblasty
   3)Identifying cardamine plants

Seeds collected -25/7/2021
No of seeds on one seed pod-19
Date of sawing Cardamine seeds- -25/7/2021
Seeds germinated -17
Date:14/8/2021
Cotyledonary stage+ 3 leaf stage.
Time:6:48pm
Place: Mampilly
Data collector- Lakshmy @Arunan HBSCE @Arunima SN Nattika Cardamine @KIRAN CUBE
[8:27 PM, 8/14/2021] Sidhy Kerala Drosophila: On a paper which is kept on a book on the table .
[9:39 PM, 8/14/2021] Sidhy Kerala Drosophila: If the flower parts get dried , it will be difficult for the larvae to feed on it. It was necessary to make a set up for keeping the flower parts fresh with moisture. So , I made a goof up.

Inside the bottle ,keep water in a bottle cap and soak cotton or tissue paper in it . And keep the cut end of the pedicel dipped or embedded in that cotton. I think , this will help to prevent drying of flower parts in a fruit fly culture bottle. But it was to be done as an initial step of setting up the trap.

Now it is not dried. To prevent drying in the coming days , I am planning to keep the bottle in water or amidst wet cotton. Don’t know this will help or not. Got only this idea.

[KiranKalakotiR (Kiran Kalakoti)]

14th August 2021, 9.30pm to 15th August 2021, 9.30pm
[10:17 PM, 8/14/2021] Saida Sayyed: 513th day of CUBE chatShaala: 14th August 2021
26 CUBists from 17 CUBE centres had joined the webinar had joined the webinar

INDIA

Delhi
Aastha Ahuja: ANDC
Raj Yashwantrao Patil

Haryana
Aashi: Panipat

Kerala
Aswathy Suresh: Thriprayar
Hemant Jeevan Magadum: trivandrum
Lakshmy PJ: Kandassankadavu
Sandra NS: Thrissur
Seethalakshmi CR: Pazhuvil, Trissur
Sidhy PP: Thanniam, Thrissur

Maharashtra
Abhijeet Singh: Navi Mumbai
Arunan MC: Breach Candy, South Mumbai
Isha Pawle: Worli, Mumbai
Janhvi Deshmukh: Wardha
Sachi Bind
Saida Sayyed: Bandra, Mumbai
Shraddha Sonavane: Panvel
Shruti Choubey: Ambernath
Vanshika Punjabi: CHM College, Ulhasnagar

Uttar Pradesh
Kiran Yadav: M…
[11:45 PM, 8/14/2021] Abhijeet Singh: Summary of Today’s Chatshaala(14th August 2021)

Today, chatshaala started with my(Abhijeet) journey in Cube since I joined i.e from 7th std. So I joined and started working on Mosquito project with my friend collaborators from my school only. We used to catch mosquitoes collaboratively and individually at school and our respective homes. The aim was to predict that in which month the number of mosquitoes will increase (because of which we maybe able to see increase in the number of mosquito born diseases for example malaria etc) in our locality so that we can prevent it from happening by destroying the possible breeding site of mosquitoes which includes water collected in thrown water bottle, coconut shell, pot holes besides roads, gardens and anywhere were water is collected as mosquitoes lay eggs in that.
We also worked on Earthworm, daphnia culturing, and snails.
Till 9th I worked then took break for 3 years for 10th and 12th board exams.

After that we discussed about what Ashwathy has been doing since when she joined that is I guess April 2020. Discussion on trapping was done then ashwathy told that she was mainly trying to find Drosophila Melanogaster from the traps through which she used to catch fruitflies. Discussion on why D.melanogaster is taken as model organism was done and turns out that it genes similar to that of humans and the sleep-wake cycle or activity rythm too Also many nobel prize has been announced with D.melanogaster studies

We can onto the discussion that what’s the difference between Model organism and Model system. At 1st we were confused with the terms but after discussion we came to an understanding that both are one and the same it’s just how an individual keeps it in front of us with respect to the organism he/she is working with.

Again we came to discuss the activity pattern or rythm of fruitflies with the help of Ashwathy’s work. So ashwathy tried to explain it with the help of a setup. So what we get was that Fruitflies are active during day time and very much or almost inactive at the night time as of with humans.
[11:47 PM, 8/14/2021] Abhijeet Singh: The whiteboard image will be shared soon.

Please add your comments @Aswathy Suresh CUBE @Saida Sayyed @Batul Pipewala Kolkata @Lakshmy PJ CUBE @Arunan HBSCE

[2:02 PM, 8/15/2021] Lydia cUBe Drosphila: Interesting Chatshaala board discussions!
What was the context for discussing imaginal disc?
[2:05 PM, 8/15/2021] Lydia cUBe Drosphila: Interesting!
Looking for more information on this
Biomimetics is a field that is gaining more interest these days.
Are there any current studies that are going on in this area?!
[2:08 PM, 8/15/2021] Lydia cUBe Drosphila: Yes
Do you have cultures that are working well for a month or more?
Would be good to show others as a proof!
[2:12 PM, 8/15/2021] Lydia cUBe Drosphila: Okay
So why not track the history of those flies? We can find out the exact reason for the death of the flies so that it does not repeat again
[2:14 PM, 8/15/2021] Lydia cUBe Drosphila: Great
Have you made a plan for that?
Why not send a detailed plan as to how you will go about ahead?
All the collaborators will also help you
[2:21 PM, 8/15/2021] Lydia cUBe Drosphila: Thank you, Abhijeet, for this summary!
What do you think of Aswathy’s circadian rhythm studies on fruit flies? What were the learnings from yesterday’s discussion?
VIDEO SHARED BY BATUL AT 2.49PM
BREAKING NEWS Dense growth of nematodes on raw potato slice B subculture observed using magnifying glass of 10X and phone camera 4X .The set up was started on 6/8/21 nine days ago with 4drops of milk and nematodes from original culture started with soil.Cubehomelab Kolkata 15/8/21Batul
[2:51 PM, 8/15/2021] Abhijeet Singh: Yes @Lydia cUBe Drosphila di.
This is interesting indeed!
Yes like humans now tend to look towards nature for the solutions of modern issues, that nature has been doing for years!
So biomimetics came up with that only I guess.

Just to give you context about how this discussion started at 1st place is that, my mom planted some karela seeds last week June or something. After a week or two I saw that how beautifully they are growing and I posted pictures in the group (Earlier chat, please follow the thread to see it). So @Arunan HBSCE found some paper with how these climbing plants are becoming an inspiration for the development of robots to face the modern issues. I’m going through that paper only actually.

As far as current studies are concerned, I didn’t look for them yet. I did some random searches to find if something has achieved in this feild but I’ve look for it more clearly. I’m going through the paper that sir sent and trying to understand the technique and methodologies they are using to do so…

I’ll keep following up this thing and look for more references! Please keep asking me for follow ups if I left somewhere. @Lydia cUBe Drosphila
[3:03 PM, 8/15/2021] Abhijeet Singh: The work that @Aswathy Suresh CUBE di is doing since last year is incredible. The activity pattern or circadian rythm is very intersting.
The thing that I’m actually amazed of is that she has been culturing the fruitflies which she caught last year. And she told that she has cultured over 40 generation of flies which is absolutely amazing.
This is gr8 @Aswathy Suresh CUBE . Please let me know what are your further plans with it!!

Learning that we got was that it is absolutely okay to get confused with terms which we think we know. As we were discussing about Model Organisms and Model System so getting confused is okay. But the thing we should actually address these things often to be clear in the end.
[3:08 PM, 8/15/2021] Arunan HBSCE: Camera is shaking. Please take better photos. @Batul Pipewala Kolkata
[3:13 PM, 8/15/2021] Arunan HBSCE: @Abhijeet Singh Please keep discussing with others, while you read the paper and say what you understood and where you are not clear.
Let’s do it collaboratively, by making mistakes and progressing together! @Abhijeet Singh @Lydia cUBe Drosphila
[3:18 PM, 8/15/2021] Arunan HBSCE: Yes, getting confused is not only ok, but knowing that recognition of our confusion is the first step towards acquiring clear knowledge What fo you say? @Abhijeet Singh @Aswathy Suresh CUBE @Lydia cUBe Drosphila
Open discussion clarifies and leads to constructing knowledge!
What do you say? @Abhijeet Singh
[3:20 PM, 8/15/2021] Arunan HBSCE: Please post photo updates and further plan of work in your setting up of Single Line Cultures of Tanniam Fruitflies @Sidhy Kerala Drosophila
[3:22 PM, 8/15/2021] Arunan HBSCE: Please share the details of these to establish better collaboration. @Lakshmy PJ CUBE
What are s heteroblasty and it’s significance?
[5:00 PM, 8/15/2021] KIRAN CUBE: INVITATION TO JOIN TODAY’S CUBE CHATSHAALA

Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in CUBE what’s app groups after the session

Webinar Link
https://webinar.hbcse.tifr.res.in/b/kir-vgc-6jf

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
VIDEO SHARED BBY BATUL AT 5.44PM
Video of subcultureB with dense nematode growth started on 6thaug on raw potato ,nine days ago with nematodes from original culture and 4drops of milk added on first day.Thereafter 2drops of water added everyday.Cube Homelab Kolkata BatulP 15/8/21
Better video☝🏼

MOSQUITO prevention and control. MOSQUITO caught by claptrap.
Location- gwaltoli.
Dated 15.08.21
Time 4.00pm
Data pic by vaibhavi chaurasia class 10
Cube kanpur.

[KiranKalakotiR (Kiran Kalakoti)]

15th August 2021, 9.30pm to 16th August 2021, 9.30pm
[9:50 PM, 8/15/2021] Arunan HBSCE: Don’t you think the background if changed to black, we could get diagnostic features of this mosquito? @Hina Mudgal Kanpur CUBE
What do you think? @Hina Mudgal Kanpur CUBE
[9:52 PM, 8/15/2021] Arunan HBSCE: What do you think about the photo?How can we improve by simple technique? @Hina Mudgal Kanpur CUBE @Saida Sayyed @Aswathy Suresh CUBE @Abhijeet Singh @Shraddha Sonavane CUBE @KIRAN CUBE
[10:40 PM, 8/15/2021] Lydia cUBe Drosphila: Great!!
A beautiful context for inspiration to understand more on biomimetics!
I too had karela plant in my house balcony. I don’t have them now… But now I’ll also plant some karela seeds and try to correlate and see if I can find something interesting!
Even I’ll look on this topic more w.r.t. to current studies in this field…we can find something and understand together!
[10:43 PM, 8/15/2021] Lydia cUBe Drosphila: Yes that’s really great!
We need to follow up with @Aswathy Suresh CUBE so that she can do more such works and inspire other collaborators as well! Follow up on other collaborators as well! @Yash Sheregare CUBE too is having an incredible fruit fly culture with him! Looking forward to current updates from all the collaborators!

So, what’s the difference between model organism and model system?
[10:44 PM, 8/15/2021] Lydia cUBe Drosphila: Yes Sir!
That’s what I also said!
I too got interested in this topic! Even I’ll look for papers related to current studies or the studies that have been done in this field!
[10:48 PM, 8/15/2021] Lydia cUBe Drosphila: That’s what we majorly do in CUBE!
Celebration of our goof-ups!
Confusion is sometimes considered a mistake by some. Acknowledging those downfalls, confusions and mistakes helps us in understanding things in a better way! @Aswathy Suresh CUBE @Abhijeet Singh @Yash Sheregare CUBE
[11:40 PM, 8/15/2021] Saida Sayyed: 514th day of CUBE chatShaala: 15th August 2021
19 CUBists from 17 CUBE centres had joined the webinar had joined the webinar

INDIA

Assam
Ishita Sonowal: Jorhat

Bihar
Shalu Sinha: Patna

Delhi
Aastha Ahuja: ANDC
Dr P. Chitralekha: DSC, Delhi

Haryana
Aashi: Panipat

Kerala
Aswathy Suresh: Thriprayar
Lakshmy PJ: Kandassankadavu
Seethalakshmi CR: Pazhuvil, Trissur

Maharashtra
Abhijeet Singh: Ulwe, Navi Mumbai
Arunan MC: Breach Candy, South Mumbai
Saida Sayyed: Bandra, Mumbai
Shraddha Sonavane: Panvel

Uttar Pradesh
Kiran Yadav: Moradabad

West Bengal
Batul Pipewala : Kolkata

Sunita rajbhar
Nishani Singh
Nalini Singh
Prachi Pant:

SRI LANKA
Nis…
[11:43 PM, 8/15/2021] Seethalakshmy CUBE: Which mosquito is this? can you take good photos of it. This is not clear!
Just focus and try to take good photos
@Hina Mudgal Kanpur CUBE ma’am

VIDEO SHARED BY JANHVI AT 9.03AM
Location: yavatmal. Date :16/08/2021. Data collector: janhvi deshmukh I am in my aunt house yavatmal I seen so many phylluntus growing here and when I see some features like five petals , symmetrical vein of leaf …so it’s a phylluntus amarus … Janhvi deshmukh homelab bajaj collage of science wardha maharshtra
PHOTOS OF PHYLLANTHUS SHARED BY JANHVI AT 9.03AM
[10:00 AM, 8/16/2021] Shraddha Sonavane CUBE: @Janhvi Deshmukh what you mean by symmetrical vein?

What are other characteristics of Phyllanthus amarus?
[10:10 AM, 8/16/2021] Shraddha Sonavane CUBE: What is difference between Petals & Tepals?
[10:10 AM, 8/16/2021] Shraddha Sonavane CUBE: Which are others species of Phyllanthus having disymmetrical vein?
[10:10 AM, 8/16/2021] Shraddha Sonavane CUBE:
great!! You got many Phyllanthus.

What is your next plan?
[10:42 AM, 8/16/2021] Abhijeet Singh: In Black background we would be easily able go distinguish the white stripes. That is how we used to do in school, when we did mosquito project. @Hina Mudgal Kanpur CUBE Ma’am

[10:00 AM, 8/16/2021] Shraddha Sonavane CUBE: @Janhvi Deshmukh what you mean by symmetrical vein?

What are other characteristics of Phyllanthus amarus?
[10:10 AM, 8/16/2021] Shraddha Sonavane CUBE: What is difference between Petals & Tepals?
[10:10 AM, 8/16/2021] Shraddha Sonavane CUBE: Which are others species of Phyllanthus having disymmetrical vein?
[10:10 AM, 8/16/2021] Shraddha Sonavane CUBE:
great!! You got many Phyllanthus.

What is your next plan?
[10:42 AM, 8/16/2021] Abhijeet Singh: In Black background we would be easily able go distinguish the white stripes. That is how we used to do in school, when we did mosquito project. @Hina Mudgal Kanpur CUBE Ma’am

VIDEO AND PHOTO SHARED BY BATUL AT 10.44AM
Nematodes transferred from subculture B started on 6th Aug on raw potato to subcultures C1 and C2 today 16/8/21.Both C1 and C2 are raw potato slices on which 4drops of milk was added one day before.Cube homelab Kolkata Batul P 16/8/21
Subcultures C1 and C2 started today with nematodes from subcultureB 16/8/21Cube Homelab Kolkata BatulP
Phylluntus urinaria @Shraddha Sonavane CUBE
Aditiya Joshi:

[11:11 AM, 8/16/2021] Aditiya Joshi CUBE: Objective: to keep the coconut water for fermenting (toddy) and try to grow the yeast/bacteria/fungi from the fermented coconut water on boiled potato slice.
Setup: two transparent plastic bottles (250ml). These were washed using dilute soap solution to clear the remnants of the contents. Then washed with water 4 times properly to clear all the soap. Dried overnight. Coconut water was collected in a container and then poured in these two bottles. One is kept open and other us capped. Kept by a wall in balcony.
Expectation: the coconut water in open bottle will show bubbles, change in smell and color indicating fermentation after 24 hours. The coconut water in capped bottle too will show these signs of fermentation but lesser compared to open bottle due to less exposure to microbes.
Date: 16/8/2021
Time: 10:40am
Location: Kala Talav, Kalyan west. @Arunan HBSCE @Saida Sayyed @Lakshmy PJ CUBE @KIRAN CUBE @Aswathy Suresh CUBE @Seethalakshmy CUBE @Batul Pipewala Kolkata and all others.

[11:11 AM, 8/16/2021] Aditiya Joshi CUBE: Coconut water filled in bottlee
PHOTO: Bottles kept for drying after washing. The white container will be used to keep the potato slices after inoculation
PHOTO: Collection of coconut water
[11:16 AM, 8/16/2021] Seethalakshmy CUBE: Great @Aditiya Joshi CUBE.
[11:18 AM, 8/16/2021] Shraddha Sonavane CUBE: Are you sure? @Janhvi Deshmukh
What do you think
Is it unequal leaf vein or leaf base?
[11:20 AM, 8/16/2021] Seethalakshmy CUBE: What do you mean by colour change?
Can you tell us about it?
[11:20 AM, 8/16/2021] Janhvi Deshmukh: Leaf base @Shraddha Sonavane CUBE
[11:21 AM, 8/16/2021] Shraddha Sonavane CUBE:
[11:28 AM, 8/16/2021] Aditiya Joshi CUBE: What I think or expect is, the coconut water will become more turbid. Color change here was meant by this.
[11:33 AM, 8/16/2021] Aditiya Joshi CUBE: Beautiful @Abhijeet Singh
I remember this term biomimetics once used in a show on discovery chanel.
There, they referred to gecko lizards. These lizards can walk on varying surfaces including smooth glass.
So, the ppl are studing the structure and design of their legs and footpads. By this, they can make some appliances like robots or vehicles which can walk on different surfaces.
Interesting @Abhijeet Singh . Keep discussing.
[11:34 AM, 8/16/2021] Seethalakshmy CUBE: Ok
Any reference?
[11:57 AM, 8/16/2021] Aswathy Suresh CUBE: SUMMARY
CUBE - Chatshaala
15.08.2021

Discussed about smelling sense capacity of 2 different D.melanogaster one from native (brought it from kitchen area) & another from Lab cultured fruit flies more than 70years passed (CsBz melanogaster - one of the lab bred).

Long term objective :
To perform olfactory assay at range of concentrations of IAA (banana essense)
using standard CsBz 2nd instar larvae and Native Drosophila 2nd instar larvae and compare their olfactory sensitivity to comment on evolutionary changes in terms of fruit odor smelling!

Hypothesis :
Native have good smelling senses compare to Lab bred one which are passed more than 70years, 3360 generations covered.

Possibilities :
Native want to find their own food so their antenna receptors always functional . But Lab bred culture flies don’t want find their food because of availability of food, Compare to native CsBz will have less functional.

CsBz flies almost passed 3360 generation in this 70 year. So, there could be possible some mutants will be form inside the culture. Because these flies culturing in closed environment could be chance mutants will serve inside culture. [ Mutation is a big word - here less sensing smell capacity mention about mutants.

How can we check their smelling capacity through experiment!
is it possible we can do it in our home ?

Surely we can do it in our home using what are available in our home…

We can replace Lab materials in to home materials to perform this Olfactory experiment

How to calibrates olfactometer working or not through control experiment?
How can Calculate mean !? Connected Biostatistics

Yesterday discussion summary - 15.08.2021

@Arunan HBSCE sir @Abhijeet Singh @Saida Sayyed @Shraddha Sonavane CUBE @Seethalakshmy CUBE @Aastha Ahuja Cube @Yash Sheregare CUBE @Lydia cUBe Drosphila mam…please add your points.
[12:24 PM, 8/16/2021] Lydia cUBe Drosphila: Any pictures to prove this claim? Would be helpful for your collaborators!
[12:25 PM, 8/16/2021] Lydia cUBe Drosphila: Nice setup!
What’s your plan with this Aditya?
And on what basis did you select coconut water for yeast/bacterial growth? Any reference or any experience/context behind this
[12:26 PM, 8/16/2021] Lydia cUBe Drosphila: What is your plan with potato slices?
[12:31 PM, 8/16/2021] Lydia cUBe Drosphila: Ohh nice! Can this be connected to the model systems that we all work on? A context to curriculum area of Biomimetics connected to any of the model systems? Just thinking
[12:32 PM, 8/16/2021] Lydia cUBe Drosphila: Thank you @Aswathy Suresh CUBE for the summary!
What’s the plan of all fruit fly collaborators for carrying out the olfactory experiment at home? How is it possible? And most importantly why do we have to do this experiment?
[12:42 PM, 8/16/2021] Arunan HBSCE: Please give a summary of your work when you were in school, especially with reference which months you got to see Aedes mosquitoes, more and in which months less? @Abhijeet Singh
[12:44 PM, 8/16/2021] Arunan HBSCE: Not sure, whether you were able to transfer one nematode? @Batul Pipewala Kolkata
[12:45 PM, 8/16/2021] Arunan HBSCE: What is your objective?
What was your design? @Batul Pipewala Kolkata
[12:48 PM, 8/16/2021] Arunan HBSCE: @Aditiya Joshi CUBE please ost daily updates to inspire others. @Aditiya Joshi CUBE
[12:48 PM, 8/16/2021] Arunan HBSCE: post*
[12:50 PM, 8/16/2021] Aswathy Suresh CUBE: Yes, currently i have 2 good subcultures SLBK B19, E15 - 46 days old culture (subculture started date - 02.07.2021) more than passed 1 month have Larvae, Unhatched pupae, also have adult flies as well inside culture.
[12:51 PM, 8/16/2021] Aswathy Suresh CUBE: How can we found what could be the reason that those flies death?
[12:54 PM, 8/16/2021] Arunan HBSCE: In that case, why not repost with this clarification using precise terminology. @Aditiya Joshi CUBE
[12:58 PM, 8/16/2021] Aswathy Suresh CUBE: I didn’t made a any proper plan ma’am, (Because even making more subculture thats also make more difficulty finding more bottles.) So Iam thinking which culture facing major problems… only that culture can make more subculture in order to save the progeny’s of particular single line cultures.
[1:09 PM, 8/16/2021] Aswathy Suresh CUBE: Which mosquito is this? @Hina Mudgal Kanpur CUBE mam
[1:13 PM, 8/16/2021] Aswathy Suresh CUBE: Nice setup @Aditiya Joshi CUBE . What is your expectations?
[1:19 PM, 8/16/2021] Aswathy Suresh CUBE: Is there any special breeding season in Aedes mosquitoes? @Dr. Sarita kumar Delhi CUBE mam
[1:43 PM, 8/16/2021] Dr. Sarita kumar Delhi CUBE: Aedes breeds best at 27 degree Celsius and 80-85% humidity. Rainy season with high humidity, and optimum temperature is best…
[1:49 PM, 8/16/2021] Abhijeet Singh: Yes yes! Ashwathy to me about the culture that @Yash Sheregare CUBE is having.

So like as I mentioned earlier that getting confused with terms os okay and only an open discussion can help us to get through with it!

So what we get to know is that there’s no difference in Model system and model organism. It’s just the matter of terms and how an individual express it to us.

So model system or model organism is a non-human species that is extensively studied to understand particular biological phenomena, with the expectation that discoveries made in the model organism will provide Insight into the workings of other organisms

To elaborate :- if anyone want to study a biological process and in order to do that he/she selects a particular species so that species …
[1:53 PM, 8/16/2021] Abhijeet Singh: Yes yes!
That’s exactly what biomeimetics is all about!

Yes I’ll keep Posting my understanding about it!
Please get the discussion going @Aditiya Joshi CUBE !!
[2:06 PM, 8/16/2021] Arunan HBSCE: Do t you think it is high time you subculture these flies of 46 day old​:upside_down_face: culture? @Aswathy Suresh CUBE
[2:26 PM, 8/16/2021] Batul Pipewala Kolkata: I am not making a claim of transferring a single nematode because larvae could be attached to even one nematode picked up from the potato surface.
[2:28 PM, 8/16/2021] Batul Pipewala Kolkata: My objective is to maintain the nematodes isolated from the soil sample.As one potato slice culture begins to die in around 14days I have transferred the nematodes before that stage occurs to fresh potato slices C1and C2
[2:55 PM, 8/16/2021] Seethalakshmy CUBE: Sir,
When vinegar add to the Milk

This is a chemical reaction. In this activity, the milk is heated and then mixed with the white
vinegar or lemon juice, which is an acid. The heat and the acid cause the protein casein in the
milk to denature, or break apart. This is a change on the molecular level. Chemical changes
occur on the molecular level, while physical changes remain the same on the molecular level.
[2:58 PM, 8/16/2021] Seethalakshmy CUBE: Milk contains a protein called casein and it is negatively charged….vinegar contain hydrogen ions which are positively charged. Hence when u mixed these two together the hydrogen ion and the casein protein attract each other and form white plastic like lumps called curd and the process is called curdling.

https://www.quora.com/What-happens-when-you-mix-milk-with-vinegR

PHOTO BY SUNITA CHAHAR

[3:10 PM, 8/16/2021] Sunita Chahar CUBE: Birds nest fungi in our garden
[3:10 PM, 8/16/2021] Sunita Chahar CUBE: Bhandup Mumbai
[3:11 PM, 8/16/2021] Seethalakshmy CUBE: Wow!! What is this? @Sunita Chahar CUBEma’am
[3:17 PM, 8/16/2021] Seethalakshmy CUBE: The curd is produced due to the presence of reaction between lactic acid bacteria and the milk protein casein. Lactic Acid or milk acid is an organic acid with chemical formula C3H6O3. When milk sugar or lactose undergoes fermentation, the product obtained is lactic acid. It is found in cottage cheese, leban, sour milk, yogurt, and Koumiss.
[3:22 PM, 8/16/2021] Arunan HBSCE: But, we can do that too, meaning, transferring a single nematode for culturing whole generations from that. What about that? @Batul Pipewala Kolkata What precautions?
[3:29 PM, 8/16/2021] Arunan HBSCE: What do you mean by denaturing of Casein protein? @Seethalakshmy CUBE
[3:37 PM, 8/16/2021] Arunan HBSCE: Is there a connection between Lactic acid of curd and Acetic acid of Vinegar in curdling? @Seethalakshmy CUBE
[3:39 PM, 8/16/2021] Seethalakshmy CUBE: Sir,
Denaturation, in biology, process modifying the molecular structure of a protein. Denaturation involves the breaking of many of the weak linkages, or bonds (e.g., hydrogen bonds), within a protein molecule that are responsible for the highly ordered structure of the protein in its natural (native) state. Denatured proteins have a looser, more random structure; most are insoluble. Denaturation can be brought about in various ways—e.g., by heating, by treatment with alkali, acid, urea, or detergents, and by vigorous shaking.

Reference : Denaturation | Definition, Examples, & Facts | Britannica
[3:41 PM, 8/16/2021] Arunan HBSCE: How do you explain in your language for all to understand. @Seethalakshmy CUBE
[3:43 PM, 8/16/2021] Seethalakshmy CUBE: Sir , it means The original structure of protein is changes in to another form it is irreversible.
[3:44 PM, 8/16/2021] Aditiya Joshi CUBE: @Aswathy Suresh CUBE I expect to see fermentation in both the bottles. But more fermentation will be seen in the open bottle than the closed. Or the open bottle will show fermentation sooner than the later.
Then I will be spreading the fermented coconut water from both bottles on the boiled potato slices and try to see if there are colonies of the yeasts/bacteria from fermented coconut water grown on the potato slice.
[3:45 PM, 8/16/2021] Seethalakshmy CUBE: Irreversible means - they can’t reach previous form
[3:46 PM, 8/16/2021] Arunan HBSCE: Good. But, how? @Seethalakshmy CUBE Please give an example?
[3:48 PM, 8/16/2021] Arunan HBSCE: Why not?
[3:51 PM, 8/16/2021] Arunan HBSCE: Why not?@Seethalakshmy CUBE
[3:53 PM, 8/16/2021] Aditiya Joshi CUBE: https://courses.lumenlearning.com/introchem/chapter/denaturation-and-protein-folding/

If the protein is subject to changes in temperature, pH, or exposure to chemicals, the internal interactions between the protein’s amino acids can be altered, which in turn may alter the shape of the protein. Although the amino acid sequence (also known as the protein’s primary structure) does not change, the protein’s shape may change so much that it becomes dysfunctional, in which case the protein is considered denatured.
[3:53 PM, 8/16/2021] Seethalakshmy CUBE: Sir, in chemistry we use many terms like deactive, denatured…
Here deactive means they deactive at a particular time/ particular temperature. If they reach its suitable time they active.
This process is reversible.

But denatured means they loss all capacity to form previous str. Here in the case of casein protein they denatured. By using vinegar. The action of vinegar completely changed it’s structure
[3:54 PM, 8/16/2021] Aditiya Joshi CUBE: Example of egg is given here
[3:55 PM, 8/16/2021] Aditiya Joshi CUBE: Denaturation Protein
[3:57 PM, 8/16/2021] Arunan HBSCE: Please describe in your own words for all to understand @Aditiya Joshi CUBE
Protein denaturation is also a consequence of cell death. Denatured proteins can exhibit a wide range of characteristics, from conformational change and loss of solubility to aggregation due to the exposure of hydrophobic groups. Denatured proteins lose their 3D structure and therefore cannot function.
PHOTOS SHARED BY DP AT 4.47PM: Can any of you identify this insect? The body is about 2-3 mm without the front antenna.
[4:48 PM, 8/16/2021] DP HBCSE: It has two long stripes along the body, has a relatively long head or snout.
[4:54 PM, 8/16/2021] KIRAN CUBE: S4E146 of CUBE chatShaala: Difference in the olfaction sensitivity of fruit flies

Kindly click on the above👆🏻 given link for the summary of last discussion.

Invitation to join today’s CUBE chatShaala,
Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in all CUBE what’s app groups and on metastudio after the session

Here’s the direct link of the CUBE chatShaala webinar: https://webinar.hbcse.tifr.res.in/b/kir-vgc-6jf

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
[4:59 PM, 8/16/2021] Seethalakshmy CUBE: Yes, @Aditiya Joshi CUBE we can take this example for denaturation.
The egg white contains protein ( which known as albumin )it is transparent and liquid.
When we boil/ cooking the egg white turns into opaque and soild.
This is dueto denaturation of protein.
When food is cooked, some of its proteins become denatured. This is why boiled eggs become hard.

The same process is occur when we add vinegar into milk.

I think what I said was clear. If you do not understand something, please ask.and correct me if iam wrong.
@Arunan HBSCE sir, @Aditiya Joshi CUBE, @Aswathy Suresh CUBE, @Lydia cUBe Drosphila, @KIRAN CUBE ma’am,@Batul Pipewala Kolkata ma’am, @Man Masih Beck RANCHI CUBE, @Abhijeet Singh and others
PHOTO SHARED BY Arunima AT 5.06PM:
Date:16/08/21. Time 11 45am. catterpillar psyche butterfly is attacking the cardamine plant. Arunima CUBE home lab Kozhikode Kerala
[5:23 PM, 8/16/2021] Seethalakshmy CUBE:
where did you find this?
@DP HBCSE sir
[5:32 PM, 8/16/2021] DP HBCSE: At my office. I sit in front of a window which has this tree. It was crawling and then hopped off. It didnot have any wings, so I assume it hopped and not flew.
[5:38 PM, 8/16/2021] P. Chitralekha Cube JNU: But denatured means they loss all capacity to form previous st.

Not always true. Check Anfinsen’s experiment which fetched him the 1972 Nobel prize.
[5:42 PM, 8/16/2021] P. Chitralekha Cube JNU: Except that in one temperature is changed and in the other pH. How do these changes denature proteins? Why doesn’t boiling milk denature the proteins in milk?
[5:44 PM, 8/16/2021] Seethalakshmy CUBE: Ma’am can you send reference?
[5:45 PM, 8/16/2021] P. Chitralekha Cube JNU: https://www.google.com/search?gs_ssp=eJzj4tTP1TcwNkwxzjBg9OJIzEvLzCtOzQMAPZsGRw&q=anfinsen&rlz=1CDGOYI_enIN944IN944&oq=anfinse&aqs=chrome.2.69i59j69i57j46i67j0i512j69i60l2.4849j0j4&hl=en-GB&sourceid=chrome-mobile&ie=UTF-8
[5:50 PM, 8/16/2021] P. Chitralekha Cube JNU: Anfinsen's dogma - Wikipedia
[6:00 PM, 8/16/2021] Seethalakshmy CUBE: Not all proteins are thought to be affected by temperature. Some proteins are denatured at very small temperatures. A specific temperature is required for each protein to denaturation .
[6:04 PM, 8/16/2021] P. Chitralekha Cube JNU: Why? How does higher temperature denature proteins?
[6:08 PM, 8/16/2021] Seethalakshmy CUBE: The weak forces between charged groups and the weaker forces of mutual attraction of nonpolar groups are disrupted at elevated temperatures, however; as a result, the tertiary structure of the protein is lost. … When denaturing agents are removed from a protein solution, the native protein re-forms in many cases.
[6:13 PM, 8/16/2021] P. Chitralekha Cube JNU: If temperature is lowered after denaturing the protein, will the protein regain its native form?
[6:14 PM, 8/16/2021] Seethalakshmy CUBE: That’s also a confusing factor
[6:18 PM, 8/16/2021] P. Chitralekha Cube JNU: Basic question to be addressed is why do proteins denature? Is there a reversible and an irreversible denaturation?
[6:20 PM, 8/16/2021] Seethalakshmy CUBE: The body strictly regulates pH and temperature to prevent proteins such as enzymes from denaturing.
Some proteins can refold after denaturation while others cannot.
Yes ma’am the reference says some protiens can re - gain it’s previous str.

Course Hero

[6:24 PM, 8/16/2021] P. Chitralekha Cube JNU:

PHOTOS SHARED BY SEETHALAKSHMI AT 9.13AM
[9:22 PM, 8/16/2021] KIRAN CUBE: VOTE NOW! Name Our Black Crazy Ant Colony [Interactive Video] - YouTube

[9:23 PM, 8/16/2021] Ankit Yadav Xms Work Shop Cube Patkar College: This paper is regarding imaginal discs with special reference to fruit fly
Imaginal disc r sac like structure found inside larva of insects that undergo metamorphosis.
These discs decide external structures such as head, thorax, limbs and genitalia.
It is still a mystery that how these discs decides fate of an insect. The reason imaginal discs are of such interest to developmental biologist is that it provide a powerful model to study multiple aspects of biological mechanism underlying diseases.

[KiranKalakotiR (Kiran Kalakoti)]

16th August 2021, 9.30pm to 17th August 2021, 9.30pm
[9:23 PM, 8/16/2021] Ankit Yadav Xms Work Shop Cube Patkar College: This paper is regarding imaginal discs with special reference to fruit fly
Imaginal disc r sac like structure found inside larva of insects that undergo metamorphosis.
These discs decide external structures such as head, thorax, limbs and genitalia.
It is still a mystery that how these discs decides fate of an insect. The reason imaginal discs are of such interest to developmental biologist is that it provide a powerful model to study multiple aspects of biological mechanism underlying diseases.
[9:34 PM, 8/16/2021] Seethalakshmy CUBE: Crazy Ants
[10:17 PM, 8/16/2021] Aswathy Suresh CUBE: 515th day of CUBE ChatShaala: 16th August 2021
24 CUBists from 24 Centres had joined the webinar.

INDIA

DELHI
DSC, Delhi : Dr P. Chitralekha

GOA
Goa : Diksha Gaunker

JHARKHAND
Kokar, Ranchi : Man Masih Beck

KERALA
Thriprayar : Aswathy Suresh
Pazhuvil, Thrissur : Seethalakshmi CR
Palakkad : Shrudhiga
Thanniam, Thrissur : Sidhy PP
Alapad, Thrissur : Vishnupriya CJ

MAHARASHTRA
Kalyan : Aditya Joshi
Breach Candy, South Mumbai : Arunan MC
Worli, Mumbai : Isha Pawle
Govandi, Mumbai : Rafikh Shaikh
Titwala : Ritik Baviskar
Dahanu Road, Palghar : Sachin Pradhan
Panvel : Shraddha Sonavane
NES Ratnam College, Mumbai : Sunita Chahar
Prabhadevi, Mumbai : Yash Sheregare

TAMIL NADU
PSGR Krishnammal College Coimbatore : Arunima

UTTAR PRADESH
Moradabad : Kiran Yadav
Uttar Pradesh : Ankit Yadav

WEST BENGAL
Kolkata : Batul Pipewala

: C L A B
: Deeksha pandey
: Prachi Pant

Note
Cubist(s) who had joined today but their name isn’t included in the list, kindly replied to this message.
[10:57 PM, 8/16/2021] Lydia cUBe Drosphila: Good!
Please post pictures and videos of these cultures. Looking forward to it.
[11:03 PM, 8/16/2021] Lydia cUBe Drosphila: You might be having dates of all those cultures bottles.
We’ll track each bottle one by one
For eg. If B is the bottle in which your flies died, then write a post here of the B bottle specifically with date of transfer of flies into B bottle, number of flies transferred, bottle name from which the flies where transferred (suppose A) into B bottle, then dates of when larvae, pupae were seen (if seen) in bottle B.
Similarly, write the history of bottle A as well from which the flies were transferred into B.
[11:10 PM, 8/16/2021] Lydia cUBe Drosphila: Okay…yes that is a problem
How many culture bottles do you have now?
Please give the names of your culture bottles one by one with date of transfer of flies of that bottle + number of flies in that bottle (females, males, gravid)
[11:16 PM, 8/16/2021] Lydia cUBe Drosphila: Yes
I had a doubt❓
Tell me what you think
Can we call a plant that we study a model organism? Or would it be called a model system? Drosophila, Moina, mosquito, snails can be called model organisms, but what about Phyllanthus, Cardamine? What do you think? Will there be a difference in the terms model organism and model system in this case?
[11:23 PM, 8/16/2021] Lydia cUBe Drosphila: Does this happen all the time? If a change in the original structure of a protein occurs, is this change always irreversible?
[11:27 PM, 8/16/2021] Lydia cUBe Drosphila:
What if you keep a potato slice just like that without spreading the fermented coconut water on it? What do you expect? Do you plan to keep a potato slice as a control?
[11:28 PM, 8/16/2021] Aditiya Joshi CUBE: Yes
[11:29 PM, 8/16/2021] Aditiya Joshi CUBE: A non inoculated slice

VIDEOS SHARED BY ASWATHY AT 12.49 AM
[12:06 AM, 8/17/2021] Aswathy Suresh CUBE: Example of 46 days old bottles - SLBK E15

started date of culture : 02.07.2021

17/08/2021
No of Flies : ~60
No of Pupae : ~150
No of Unhatched Pupae : ~40
No of Hatched Pupae : ~110
No of Dead Flies : ~50

Condition of media : Solidified.

Location : Bottukadavu - Thrissur Kerala.
[12:13 AM, 8/17/2021] Aswathy Suresh CUBE: 29 Days old culture bottle - SLBK E16

started date of culture : 19.07.2021

17/08/2021(today)
No of Flies : ~60-65
No of Pupae : ~120
No of Unhatched Pupae : ~40
No of Hatched Pupae :
~80
No of Dead Flies : ~30

Condition of media : Solidified.

Location : Bottukadavu - Thrissur Kerala.
[12:14 AM, 8/17/2021] Seethalakshmy CUBE: No @Lydia cUBe Drosphila

The body strictly regulates pH and temperature to prevent proteins such as enzymes from denaturing.
Some proteins can refold after denaturation while others cannot.
The reference says some protiens can re - gain it’s previous str.

Course Hero

[12:25 AM, 8/17/2021] Seethalakshmy CUBE: Good question @Abhijeet Singh
Yeah we can look their morphological features.
That’s actually iam trying to find.
What got from references
Back crazy ants have
Non- clubed antenna
Size:2.3- 3 mm( worker)
And their movement ( random movement )
[12:31 AM, 8/17/2021] Aswathy Suresh CUBE: SLBK A22
subculture started date : 12.08.2021

Update : Fruit Flies

Transfer Flies : 1 Fly (Look Like Gravid One)

Expectations :
16.08.2021 : ~30 Eggs + ~20 Larvae + ~10 Pupae (on 5th day expectations)

Observation : 5th Day
17/08/2021
No of Eggs : ~5
No of Larvae : 0
No of Pupae : 0
No of Flies : 1 (Transferred one)

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
[12:36 AM, 8/17/2021] Aswathy Suresh CUBE: SLBK B21
subculture started date : 12.08.2021

Update : Fruit Flies

Transfer Flies : 12 Flies
Gravid Flies : 5, Male Flies : 7

Expectations :
16.08.2021 : ~60 Eggs + ~35 Larvae + ~30 Pupae (on 5th day expectations)

Observation : 5th Day
17/08/2021
No of Eggs : ~40
No of Larvae : 20
No of Pupae : 30
No of Flies : ~2 (Transferred flies)
Dead Flies : ~10

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
[12:40 AM, 8/17/2021] Aswathy Suresh CUBE: SLBK E17
subculture started date : 12.08.2021

Update : Fruit Flies

Transfer Flies : 14 Flies
Gravid Flies : 6, Male Flies : 8

Expectations :
16.08.2021 : ~70 Eggs + ~40 Larvae + ~30 Pupae (on 5th day expectations)

Observation : 5th Day
17/08/2021
No of Eggs : ~30
No of Larvae : 0
No of Pupae : 0
No of Flies : ~14 (Transferred flies)
Dead Flies : 0

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
[12:46 AM, 8/17/2021] Aswathy Suresh CUBE: Fruit flies eggs can able see in the above part of media, bottle wall sides. Video taken from SLBK E17 (subculture bottle). Date of video 16/08/2021

VIDEO SHARED BY SEETHALAKSHMY AT 12.54PM
[12:54 AM, 8/17/2021] Seethalakshmy CUBE: Update on Ant model system.

Inside my formicarium again i can see some pupae( small white ant)as well as larvae( a cylindrical shape with black dot).
Ants carrying their own larvae and pupae.
Location : Pazhuvil, Thrissur, Kerala.
Date :16/8/21.
Time :11:41 pm.
Data collector : seethalakshmi. C. R. CUBE Nattika
[1:01 AM, 8/17/2021] Seethalakshmy CUBE: A few ants hold larvae and pupae inside the formicarium and a few outside.
Location : Pazhuvil
Date:16/8/21
Time:11:43 pm
Data collector: Seethalakshmi
[1:05 AM, 8/17/2021] Man Masih Beck RANCHI CUBE: is there a way you could remove the larvae from the colony to observe the different larval stages more closely?
How many larval stages do your ants have @Seethalakshmy CUBE ?
[8:07 AM, 8/17/2021] Lakshmy PJ CUBE: Heteroblasty is the condition in which plants show a certain degree of variation among individual metamers during ontogeny. In some cases, however, there are abrupt and substantial changes in form and function (e. g. in leaf form, leaf size, phyllotaxy, internode length, anthocyanin pigmentation, rooting ability, or wood structure). These plants were called “heteroblastic” by Karl Goebel more than a century ago, but the functional significance of this type of ontogenetic change, the evolutionary trajectories in different plant groups, even their frequency in the plant kingdom are still unresolved issues. We argue that slow progress is partly due to an on-going terminological
confusion and the lack of distinction between other developmental processes such as ontogenetic drift.

https://www.researchgate.net/publication/226433716_Heteroblasty-A_Review
@Aditiya Joshi CUBE @Arunan HBSCE @KIRAN CUBE @P. Chitralekha Cube JNU @Seethalakshmy CUBE
[8:27 AM, 8/17/2021] Seethalakshmy CUBE: Figure - PMC

[8:34 AM, 8/17/2021] Seethalakshmy CUBE: I got this from above reference. But this is life cycle of camponotus ant. But didn’t get crazy-ants life cycle
[9:56 AM, 8/17/2021] Seethalakshmy CUBE: 𝗦𝘂𝗺𝗺𝗮𝗿𝘆 𝗼𝗳 515𝘁𝗵 𝗱𝗮𝘆 𝗼𝗳 𝗖𝗨𝗕𝗘 𝗖𝗵𝗮𝘁𝘀𝗵𝗮𝗮𝗹𝗮 .

Yesterday we started the discussion 𝗔𝘀𝘄𝗮𝘁𝗵𝘆’𝘀 fruitflies. She trying to bulid up olfactometer in her 𝗛𝗼𝗺𝗲 𝗟𝗮𝗯 .
As well as we talk about
𝗖𝗵𝗮𝗶 𝗮𝗻𝗱 𝘄𝗵𝘆 ?

• 𝗖𝗵𝗮𝗶 𝗮𝗻𝗱 𝘄𝗵𝘆 ?" is jointly organised by TIFR and Prithvi Theatre, Juhu. It is a monthly forum to enable informal discussions of interesting scientific issues outside a traditional academic setting.

we discussed about Ants model system.
𝗦𝗲𝗲𝘁𝗵𝗮𝗹𝗮𝗸𝘀𝗵𝗺𝗶 raised a question :The olfactometer only for fruitflies larvae? We can use this setup for other organism like Ants, snails?
So we want to check the life cycle of Ants.
Life cycle of Ants
Egg-larvae - pupae - adult
Which type of larval stage we can suggest for this experiment??
Then seethalakshmi tell about her Home Lab.
We need to find which types of ants she culture her 𝗛𝗼𝗺𝗲 𝗟𝗮𝗯 ?
How can identify an Ant species?
What are Morphological features of Ants???

Yes, we will continue the discussion…

please give your inputs
@Aswathy Suresh CUBE ,@Man Masih Beck RANCHI CUBE ,@Arunan HBSCE sir,@KIRAN CUBE ma’am,@Sunita Chahar CUBE ma’am,@Batul Pipewala Kolkata ma’am,@P. Chitralekha Cube JNU ma’am,@Shraddha Sonavane CUBE ,@Aditiya Joshi CUBE and others …
[10:48 AM, 8/17/2021] Abhijeet Singh: So,
We actually did a year around mosquito project.
We were 8-10 students from 7-9th grade. We used to catch mosquito at our school together (before and after school hours) and at our homes by clap-trap method.
We used to collect the mosquito in a small container or box and the next day we put all that mosquitoes on a black paper. So that we can distinguish between Aedes and non-aedes ones(as aedes mosquito has white stripes on their legs)

We take the total count and and aedes mosquitoes number in that. We did this whole year with the hypothesis that we will fine more number of aedes in the late monsoon months(August to September end and initial weeks of October maybe)
Basic idea behind our hypothesis was that as after rain the mosquitoes will get more…
[10:53 AM, 8/17/2021] Abhijeet Singh: I did look for pictures if there’s any in my backup or mail but I couldn’t find. I’ll try to show that if I get any reference or something.
[11:00 AM, 8/17/2021] Shivam CUBE NEMATODE: Do share White bord screenshots from yesterday’s discussion.
[11:00 AM, 8/17/2021] Abhijeet Singh: Looking forward updates from @Hina Mudgal Kanpur CUBE Ma’am .
Please let us what do you think of this everyone!!!

REFERENCE
Yesterday discussed topics related important reference given in public chats.

Chai & Why

TIFR’s unique outreach initiative since 2009 has been the “Chai and Why?” series, a forum to enable informal discussions of interesting scientific issues outside a traditional academic setting in a Science Café format.
http://univ.tifr.res.in/gs2021/Gallery/Images/022-Canteen.JPG

http://chaiandwhy.org/#:~:text=TIFR’s%20unique%20outreach%20initiative%20since,monthly%20session%20from%20September%202009

Light Receptors In Fruit Fly Larvae

The larval eye is situated anteriorly and lateral, at the tip of the larva,Larval eye of drosophila - Google Search

[11:28 AM, 8/17/2021] Seethalakshmy CUBE: thank you @Aswathy Suresh CUBE for giving white boards
[11:32 AM, 8/17/2021] Aswathy Suresh CUBE:
[12:17 PM, 8/17/2021] Abhijeet Singh: Actually that’s great point to look into this more clearly!
I think that if we go definition wise…

In biology ‘system’ is something like a group of things working together to make stuff happen that helps in growth, nutrition and reproduction.

‘organism’ is any living structure, can be plant or fungi or bacteria capable of growth and reproduction.

Drosophila, Moina, mosquito, snails can be called model organisms, but what about Phyllanthus, Cardamine?
I haven’t categorised then into ‘model system and organism’ So if your trying to convey that they are seperate please let me know how and why do you think that!?

Can we call a plant that we study a model organism? Or would it be called a model system?
I think we can call it both . Plants have differe…
[12:42 PM, 8/17/2021] Abhijeet Singh: Photos amd references will do great if you can share them with us!!
@Janhvi Deshmukh
[12:45 PM, 8/17/2021] Abhijeet Singh: So why fermented coconut water is important @Aditiya Joshi CUBE .
What ingredients do yeast or bacteria or fungii need to grow?
What is the basic idea behind fermentation!?
[12:49 PM, 8/17/2021] Abhijeet Singh: Yeahhh I was also thinking about it!!
It would be great!
But proper understanding and references in this field is what we require before. Lets tune in Chatshaala for discuss on it!! @Lydia cUBe Drosphila @Aditiya Joshi CUBE @Saida Sayyed
[12:52 PM, 8/17/2021] Abhijeet Singh: With a brush or something it would be easy to pick them up and transfer!!
@Batul Pipewala Kolkata
What do you think?
And also what are we studing in nematodes?
Can you give a context about it?
[1:21 PM, 8/17/2021] Aditiya Joshi CUBE: Actually, the traditional toddy is made from palm or coconut. There the juice/sap of palm/coconut flower. That is collected and fermented to give an alcoholic drink toddy.
About fermentation what I think is, the sugars like glucose will be converted to pyruvic acid. This pyruvic acid then will be converted into alcohol.
Yeasts/bacteria will require sugar or a carbon source, nitrogen source some ions like sodium, potassium, chloride, etc.
So, I think, the yeast/bacteria will utilise these nutrients and result in formation of alcohol.
I will try to observe the growth of these yeast/bacteria on a boiled potato slice.
[1:21 PM, 8/17/2021] Aditiya Joshi CUBE: @Abhijeet Singh
[1:37 PM, 8/17/2021] Arunan HBSCE: What do we mean by “internal interactions”?
[1:39 PM, 8/17/2021] Arunan HBSCE: How are we sure this is an insect? @DP HBCSE
PHOTO SHARED BY ADITIYA JOSHI AT 1.41PM
[1:41 PM, 8/17/2021] Aditiya Joshi CUBE: Ivy gourd vine extending ‘hands’ to grasp the support… We can see tip of tendril bit curved to creat a small hook like thing… Kala Talav, Kalyan west. 16/8/2021. 9:30 pm
[1:42 PM, 8/17/2021] Arunan HBSCE: When we boil, why egg white becomes hard?

That way, even egg yolk too becomes hard, on boiling, right? Doesn’t it? @Seethalakshmy CUBE @Aditiya Joshi CUBE
[1:47 PM, 8/17/2021] Arunan HBSCE: How are we sure this is Psyche caterpillar? @Arunima Dey Aren’t other caterpillars possible in Cardamine?
[2:04 PM, 8/17/2021] Seethalakshmy CUBE: Yes sir
[2:05 PM, 8/17/2021] Arunan HBSCE: An old CUBE saying

Confusion is the starting point of understanding. What do you say? @Seethalakshmy CUBE
[2:06 PM, 8/17/2021] Arunan HBSCE: Examples? @Seethalakshmy CUBE
[2:06 PM, 8/17/2021] Seethalakshmy CUBE: But iam not sure the egg yold contains protein like albumin.
[2:07 PM, 8/17/2021] Seethalakshmy CUBE: Sure sir, I agree to this.
[2:14 PM, 8/17/2021] Arunan HBSCE: As Lydia wants to know, what do you expect in this Control slice? @Aditiya Joshi CUBE
[2:17 PM, 8/17/2021] Arunan HBSCE: Ooooh! No larvae mentioned! @Aswathy Suresh CUBE
[2:19 PM, 8/17/2021] Seethalakshmy CUBE: Yes sir, the egg yolk contains protein but at same time it contains lots of fat.
[2:20 PM, 8/17/2021] Arunan HBSCE: Interesting. Don’t you expect any larvae?
Aren’t you surprised? @Aswathy Suresh CUBE And do we still call it a healthy culture? @Aswathy Suresh CUBE @Lydia cUBe Drosphila @Yash Sheregare CUBE @Sidhy Kerala Drosophila @Man Masih Beck RANCHI CUBE @Aastha Ahuja Cube
[2:20 PM, 8/17/2021] Batul Pipewala Kolkata: can we remove the larvae and pupae and click their pics?Can we collect the pupae in a separate bottle and observe its metamorphosis
[2:22 PM, 8/17/2021] Arunan HBSCE: No larvae mentioned? Why? How can we call as healthy culture? @Aswathy Suresh CUBE
[2:27 PM, 8/17/2021] Arunan HBSCE: How about photo evidence of eggs? @Aswathy Suresh CUBE All are very eager to see.@Aswathy Suresh CUBE

VIDEO SHARED BY BATUL AT 2.28PM

Dense Nematode culture on raw potato slice started on 6thAug,eleven days old.On first day 3to4 drops of milk was added and nematodes transferred from original potato slice that had soil.After that only two drops of water added everyday.Cube Homelab Kolkata BatulP 17/8/21
[2:32 PM, 8/17/2021] Arunan HBSCE: Congrats! @Aswathy Suresh CUBE This is the stock culture you saved because of collaborative interaction and help from others. @Aswathy Suresh CUBE Please comment.
[2:38 PM, 8/17/2021] Arunan HBSCE: What about at least two of the latest SLBK C bottles? @Aswathy Suresh CUBE
Every one is eager. In fact please post state of all SLBK C bottles. @Aswathy Suresh CUBE
What do you say? @Lydia cUBe Drosphila @Yash Sheregare CUBE @Aastha Ahuja Cube @Raj Yashwantrao Patil CUBE @KIRAN CUBE
[2:40 PM, 8/17/2021] Arunan HBSCE: Why are the ants going out?@Seethalakshmy CUBE Look at 9 seconds.
[2:42 PM, 8/17/2021] Arunan HBSCE: Are you sure pupae will look like white ants? Any reference? @Seethalakshmy CUBE
[2:44 PM, 8/17/2021] Arunan HBSCE: Please give all essential details of which ant, Date, Time, Location of CUBEHomeLab, Name of Data collector along with each photo. @Seethalakshmy CUBE
[2:47 PM, 8/17/2021] Arunan HBSCE: More confusing!Please describe in your own words and of course with reference link. @Lakshmy PJ CUBE
What is the Context? @Lakshmy PJ CUBE
[2:47 PM, 8/17/2021] Arunan HBSCE: More confusing!Please describe in your own words and of course with reference link. @Lakshmy PJ CUBE
What is the Context? @Lakshmy PJ CUBE
[2:48 PM, 8/17/2021] Arunan HBSCE: Please explain in your own words, in a simple way as you understood it. @Seethalakshmy CUBE
PHOTO BY BATUL AT 2.49PM
[2:49 PM, 8/17/2021] Batul Pipewala Kolkata: No nematode growth on Rava sugar vinegar medium started on 11/8/21 Though I had reduced the vinegar in the medium nematodes did not multiply.17/8/21
[2:50 PM, 8/17/2021] Arunan HBSCE: Please ask for help, very openly, showing where are you stuck? @Seethalakshmy CUBE
[2:54 PM, 8/17/2021] Arunan HBSCE: What is Clap trap method? @Abhijeet Singh
[2:57 PM, 8/17/2021] Arunan HBSCE: Great! @Abhijeet Singh Which year you did this work? Please post some photos, as evidence, from your presentation etc. @Abhijeet Singh Please contact your collaborators too.
@Hina Mudgal Kanpur CUBE Please join in discussion.
[3:00 PM, 8/17/2021] Arunan HBSCE: Wasn’t there some different result and different conclusion came from others in your own school? @Abhijeet Singh
[3:01 PM, 8/17/2021] Arunan HBSCE: Please post some evidence. Please ask your collaborators to post to you too. @Abhijeet Singh
[3:01 PM, 8/17/2021] Arunan HBSCE: How about contacting your collaborators? Who were your collaborators? @Abhijeet Singh
[3:02 PM, 8/17/2021] Batul Pipewala Kolkata: The question arises how nematodes are surviving in @Aswathy Suresh CUBE medium TRSV? Is tomato necessary?

Glimpses of CUBE History CUBE Feb2018 HBCSE, TIFR, Mumbai
[3:12 PM, 8/17/2021] Arunan HBSCE: What is Imaginal disks? Are they imaginary? Are Imaginal disks different from larval sense organs and larval brain? @Aswathy Suresh CUBE @Seethalakshmy CUBE
[3:17 PM, 8/17/2021] Arunan HBSCE: Please comment on topics to continue discussion for othetsto understand and for us to get more clarity.
@Seethalakshmy CUBE
[3:17 PM, 8/17/2021] Arunan HBSCE: others*
[3:25 PM, 8/17/2021] Aswathy Suresh CUBE: Larvae can’t able to see inside E15 culture bottle, Bottle full covered with fruit flies pupae shell sir, so can’t able to seen them.
[3:27 PM, 8/17/2021] Aswathy Suresh CUBE: Larvae can’t able see, but lot of unhatched pupae I observed inside culture ~40. That means still some eggs,larvae also there in the culture.
[3:31 PM, 8/17/2021] Aswathy Suresh CUBE: Yes, This bottle can called healthy culture because After 46 days later still have Larvae, Unhatched Pupae Adult Flies also there. Approximately 60 Adult flies were present inside the culture. Media have no contamination, still solidified condition.
[3:35 PM, 8/17/2021] Aswathy Suresh CUBE: Most of the Larvae were present inside the media portion… That’s why I couldn’t able to count them. Approximately 20 more larvae I observed yesterday.(sorry for not mentioning larvae).
[3:45 PM, 8/17/2021] Arunan HBSCE: Don’t we have expectations of larvae in a healthy bottle brimming with flies? @Aswathy Suresh CUBE
So, when not located, as against our expectation, should we not be worried and made it as a BREAKING NEWS STORY?
What’s your comment? @Aswathy Suresh CUBE @Lydia cUBe Drosphila @Yash Sheregare CUBE @Man Masih Beck RANCHI CUBE @Raj Yashwantrao Patil CUBE @Sachin Pradhan CUBE @Aastha Ahuja Cube
[3:46 PM, 8/17/2021] Batul Pipewala Kolkata:
[3:46 PM, 8/17/2021] Arunan HBSCE: Incidentally, in this bottle SLBKE16, I was able to locate larvae at 11 seconds into the video. @Aswathy Suresh CUBE
[3:49 PM, 8/17/2021] Batul Pipewala Kolkata: Yes sir I could see 5 larvae moving in this bottle
[3:50 PM, 8/17/2021] Arunan HBSCE: Aby examples? @Seethalakshmy CUBE
What kind of protein in Casein compared to Globin? How do we describe it? @Seethalakshmy CUBE
[3:50 PM, 8/17/2021] Aswathy Suresh CUBE: Yes sir, this was one of the culture weak condition observed in last june month. It revived by the help of other collaborators @Arunan HBSCE sir @KIRAN CUBE mam @Batul Pipewala Kolkata mam @Aastha Ahuja Cube. Iam always thankful for that .
[3:52 PM, 8/17/2021] Arunan HBSCE: How do you expect to get eggs in this bottle? @Aswathy Suresh CUBE Please give your reasoning.
[3:58 PM, 8/17/2021] Arunan HBSCE: Are you not worried that there are no larvae, even after 5 days of transferring 14 flies of which 6 were gravid flies into this bottle? @Aswathy Suresh CUBE
Do you see some problem, again? @Aswathy Suresh CUBE Let’s discuss with every one.
[3:58 PM, 8/17/2021] Aswathy Suresh CUBE: Yes, I still keeping C subcultures bottles. but there is no flies, Larvae, Pupae in those bottles. Bottle where completely empty condition.
[3:59 PM, 8/17/2021] Arunan HBSCE: Good! Looking for your involvement @Shivam CUBE NEMATODE
[4:03 PM, 8/17/2021] Arunan HBSCE: Any reference for how alcohol is formed? @Aditiya Joshi CUBE @Abhijeet Singh @Seethalakshmy CUBE
[4:04 PM, 8/17/2021] Arunan HBSCE: Great! Looking for updates on other model systems including Phyllanthus of your HomeLab @Aditiya Joshi CUBE
[4:05 PM, 8/17/2021] Arunan HBSCE: Why? Any explanation? @Seethalakshmy CUBE
[4:06 PM, 8/17/2021] Arunan HBSCE: So, what exactly is your confusion about? @Seethalakshmy CUBE
[4:07 PM, 8/17/2021] Aswathy Suresh CUBE: Yes I can also see some 3rd instar larvae were curling bottle wall
[4:07 PM, 8/17/2021] Arunan HBSCE: Great idea! We need close up photos of ants too! @Batul Pipewala Kolkata @Seethalakshmy CUBE @Shrudhiga
[4:08 PM, 8/17/2021] Aswathy Suresh CUBE:
[4:10 PM, 8/17/2021] Aswathy Suresh CUBE: Imaginal disc is a sac-like epithelial structure found inside the larva of insects that undergo metamorphosis.
[4:10 PM, 8/17/2021] Aswathy Suresh CUBE: Larva turns into a pupa, almost all the larval tissues degenerate and the imaginal discs turn into the external structures of the head, thorax, limbs and genitalia.
[4:13 PM, 8/17/2021] Arunan HBSCE: Why do you think you missed it earlier?
A nice of Goof Up!

Was it because that was not part of the expectation?

What lessons can we learn and pass on to others from this, as vital lesson in Collaboration during establishing
CUBE HomeLabs! @Aswathy Suresh CUBE @Lydia cUBe Drosphila @Saida Sayyed @Aastha Ahuja Cube @KIRAN CUBE and others
[4:16 PM, 8/17/2021] Arunan HBSCE: What is the lesson in Collaboration from this we learn, as CUBE starts with Collaboratively? @Aswathy Suresh CUBE
[4:18 PM, 8/17/2021] Arunan HBSCE: Let’s highlight that lesson, for others to share their Daily Updates! @Aswathy Suresh CUBE
[4:21 PM, 8/17/2021] Arunan HBSCE: What one sees will be different from what collaborators see!
We have examples, every day!

So, please give photo evidence for your positive as well as negative observations & conclusions, for others to verify! @Aswathy Suresh CUBE
[4:23 PM, 8/17/2021] Arunan HBSCE: @Lydia cUBe Drosphila was asking for and it is good to give evidence @Aswathy Suresh CUBE Who knows, we may see some possibilities!
[4:29 PM, 8/17/2021] Arunan HBSCE: Wow!
@Batul Pipewala Kolkata
What lessons from this for all Cubists?

Surely, @Aswathy Suresh CUBE and others like you and me could come up with this because Aswathy has been consistently keeping her HomeLab active and posting Reports with photos!

Another feather in Aswathy’s cap!

What lessons we learn from this? @Batul Pipewala Kolkata @Seethalakshmy CUBE and others
[4:30 PM, 8/17/2021] Arunan HBSCE: Let’s find out approximately how many? Is it according to the expectations? @Aswathy Suresh CUBE

PHOTO SHARED BY KIRAN AT 4.46PM
[4:46 PM, 8/17/2021] KIRAN CUBE: BREAKING NEWS fruit fly has got emerge out of pupal case on 17th day But found dead. Story of pupae which were found under the lid of dustbin, 5 of the pupae were transferred in plastic container on 1st August 2021.
[5:06 PM, 8/17/2021] KIRAN CUBE: S4E146 of CUBE chatShaala: Difference in the olfaction sensitivity of fruit flies

Kindly click on the above👆🏻 given link for the summary of last discussion.

Invitation to join today’s CUBE chatShaala,
Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in all CUBE what’s app groups and on metastudio after the session

Here’s the direct link of the CUBE webinar: https://webinar.hbcse.tifr.res.in/b/kir-hye-cn4
Note: we have changed the room link to avoid technical error

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.

KOEL CALL SHARED BYE SEETHALAKSHMY AT 6.06PM
Koel cooing
Location : Pazhuvil
Date:17/8/21
Time: 4:20pm
Data collector: seethalakshmI

VIDEOS SHARED BY BATUL AT 6.11PM
Anterior end of the soil nematode family Rhabditidae showing c)straight stoma(mouth)b)enlarged oesophagus and c)pharyngeal bulb.Observation under 45X of compound microscope.The nematode has been picked up from the raw potato slice subculture B. Cube homelab Kolkata BatulP 17/8/21
Nematodes multiplying on subculture B raw potato slice observed using magnifying glass of 10X and camera zoom4X.17/8/21Cube Homelab Kolkata BatulP
Video showing movement of soil nematodes size approx 2mm under 10X of compound microscope. CUBE Homelab Kolkata BatulP 17/8/21
What about others???
Can you hear the koel cooing in your location ???
@Aditiya Joshi CUBE
@Drishtant Cube Moina, @DP HBCSE sir, @Man Masih Beck RANCHI CUBE, @Shrudhiga, @Aswathy Suresh CUBE, @Sidhy Kerala Drosophila, @Dr. Binumol SN Nattika ma’am, @Batul Pipewala Kolkata ma’am, @Arunan HBSCE sir, @KIRAN CUBE ma’am
And others
None that I can recollect. But are these Koel calls at your place consistent? or random outlier?
No Cuckoo calls heard in my locality at Kokar, Ranchi, Jharkhand.
[6:36 PM, 8/17/2021] Seethalakshmy CUBE: No @DP HBCSE sir, not regularly.
[6:37 PM, 8/17/2021] Seethalakshmy CUBE: Only in my area.
What about other places in Kerala
Ok, have the rains reduced in Kerala and weathered warmed a little? It could be some false alarm for that Koel. In Mumbai and I guess also in Ranchi, rains are still on…
Cuckoo calls heard a day before but when I was about to record it, it stopped😅
Yes, sir, it rained today as well as sunny.
[6:50 PM, 8/17/2021] Seethalakshmy CUBE: Ok, next time try to record the voice
[6:51 PM, 8/17/2021] Seethalakshmy CUBE: Ok! Is it rain there?
[6:55 PM, 8/17/2021] Seethalakshmy CUBE: Is there any connection between the rain and their calling?
yes. it rains daily in the second half of the day.
Ohk

FILE SHARED BY RAHUL AT 8.46PM
No I dont hear koels calling in my locality
happy to share with the new cubists a collaborative work on earthworm regeneration by ANDC cubists back in 2015-16
Yes please
How do you identify genus and species of earthworm??
516th day of CUBE ChatShaala: 17th August 2021.

22 CUBists from 22 Centres had joined the webinar.

INDIA

ASSAM
Jorhat : Ishita Sonowal

DELHI
DSC, Delhi : Dr P. Chitralekha

HARYANA
Panipat : Aashi

JHARKHAND
Morabadi, Ranchi : Rechel Tirkey

KERALA
Kolenchery : Aparna Saleeph
Thriprayar : Aswathy Suresh
Pazhuvil, Trissur : Seethalakshmi CR
Thanniam, Thrissur : Sidhy PP

MAHARASHTRA
Ulwe, Navi Mumbai : Abhijeet Singh
Breach Candy, South Mumbai : Arunan MC
Powai : Lydia Mathew
Mumbai : Nagarjuna G.
Mulund, Mumbai : Priti Kanade
Dahanu Road, Palghar : Sachin Pradhan
Bandra, Mumbai : Saida Sayyed
Kalyan : Shivam Kumar Sriwas
Panvel : Shraddha Sonavane

TAMIL NADU
PSGR Krishnammal College Coimbatore : Arunima

UTTAR PRADESH
Moradabad : Kiran Yadav

WEST BENGAL
Kolkata : Batul Pipewala

: Ashi
: Meena

Note
Cubist(s) who had joined today but their name isn’t included in the list, kindly replied to this message

[KiranKalakotiR (Kiran Kalakoti)]

17th August 2021, 9.30pm to 18th August 2021, 9.30pm
@Aswathy Suresh CUBE @Saida Sayyed @Abhijeet Singh @aashi.

Why finding mean is important? What’s the context of discussing mean in case of olfactometer?
@Shraddha Sonavane CUBE we all remember your wonderful work that you have done on Phyllanthus recently.

Any follow up or update on your work?.
What does this whiteboard tells us?
What’s the Chai and Why? Session
[11:32 PM, 8/17/2021] Aswathy Suresh CUBE: @Isha Pawle please give your single line cultures of fruit flies in Worli - Mumbai

•How many Single Line Culture you made? When?

•Can you please tell the names of single line culture that you made.

•How did you trapped fruit flies?

Please give your current Home Lab Updates
[11:36 PM, 8/17/2021] Aswathy Suresh CUBE: @Yash Sheregare CUBE please give the updates from your home lab fruit flies culture in Prabhadevi - Mumbai.

•How many subculture you made newly?

•What is the current condition of your single line cultures today?
[11:40 PM, 8/17/2021] Aswathy Suresh CUBE: @Janhvi Deshmukh Do you keep any new setups for to trap fruit flies in Wardha - Mumbai?

•What is your further plan?

•Do you still facing any issues while trapping fruit flies?

Please give update from your Home Lab.
[11:46 PM, 8/17/2021] Aswathy Suresh CUBE: Understanding the confusion in understanding how to calculate mean (we should highlight some of such kind of instance in chai and why? session that we take for granted)…Collaboration, Communication, curiousity, context to curriculum and consistency.
[11:51 PM, 8/17/2021] Aswathy Suresh CUBE: Chai & Why

TIFR’s unique outreach initiative since 2009 has been the “Chai and Why?” series, a forum to enable informal discussions of interesting scientific issues outside a traditional academic setting in a Science Café format.
http://univ.tifr.res.in/gs2021/Gallery/Images/022-Canteen.JPG
[12:28 AM, 8/18/2021] Seethalakshmy CUBE: I think the larvae not is visible at everytime. I didn’t much have idea about it, how can collect it’s larvae in the formicarium???
Please give some suggestions!
@Batul Pipewala Kolkata ma’am, @Man Masih Beck RANCHI CUBE, @Arunan HBSCE sir, @Shrudhiga, @KIRAN CUBE ma’am, @Vishnupriya CUBE, @Aditiya Joshi CUBE, @Sunita Chahar CUBE ma’am and others…
[12:46 AM, 8/18/2021] Seethalakshmy CUBE: The gelatin dessert “Jello” gels due to the presence of a protein. When Jello is heated, its structure is altered so that it is no longer a gel but a liquid. If the denaturing conditions reversed by cooling in the refrigerator, the protein reforms into its original gel structure.
[12:47 AM, 8/18/2021] Seethalakshmy CUBE: Yes ma’am. This is a good idea! But how can collect their larvae in to the formicarium???
As well as how can feed them?
[12:51 AM, 8/18/2021] Seethalakshmy CUBE: Yes sir, that’s iam trying to say some of them are carrying inside the formicarium, as well as they carriying to out side the formicarium.
[12:51 AM, 8/18/2021] Seethalakshmy CUBE: Yes sir!
[12:56 AM, 8/18/2021] Seethalakshmy CUBE: Yes sir! That’s i want to find. Which species of ant these???
I need others help for identifying the Ant species.
Cubists can help me to overcome this problem.
This is a major problem that i faced my Model system.
Here iam stuck!
[12:58 AM, 8/18/2021] Seethalakshmy CUBE: Yes sir! Do all ants have same type of life cycle??
Please help me to find out Crazy Ant’s life cycle.
@Batul Pipewala Kolkata ma’am, @KIRAN CUBE ma’am, @Man Masih Beck RANCHI CUBE, @Arunan HBSCE sir, @Aswathy Suresh CUBE, @Sunita Chahar CUBE ma’am, @Aditiya Joshi CUBE,@Lakshmy PJ CUBE…
And others
[1:01 AM, 8/18/2021] Seethalakshmy CUBE: Yes sir,
What is imaginal disk??
Can you explain it???
@Aswathy Suresh CUBE, @Saida Sayyed, @Abhijeet Singh, @KIRAN CUBE ma’am and others
[1:04 AM, 8/18/2021] Seethalakshmy CUBE: My confusion is how can protien get refold??
It have 2 category like reversible and irreversible reaction??
PHOTO AND VIDEO OF POTATO SLICE SHARED BY BATUL AT 10.36PM
Soil Nematodes subcultureB after 12days started on 6thAug with raw potato slice with 3to 4 drops of milk and nematodes from original culture.18/8/21Cube homelab Kolkata BatulP
Close up video of the nematodes size approx 1.5mm to 2mm using magnifying glass and mobile camera .The culture is 12days old on raw potato slice.Cube homelab Kolkata BatulP 18/8/21

After 24 hrs of fermentation. Left side bottle is open bottle. Right side bottle is closed bottle. Marked blue(left bottle/open) we can see a whitish layer formed. Marked Red(right bottle/closed) a thin whitish layer formed. Date:17/8/2021. Aditya Joshi. Kala Talav, Kalyan west

Small bubbles are seen in open bottle. Smells different from original coconut water. Somewhat smells like near bakery or smelly bread. 17/8/2021. Aditya Joshi. Kala Talav, Kalyan west. 24 hrs of fermentation
[11:07 AM, 8/18/2021] Seethalakshmy CUBE: So what is your interpretation?
[11:07 AM, 8/18/2021] Seethalakshmy CUBE: @Aditiya Joshi CUBE

48 hrs of fermentation… A thicker whitish layer in open bottle. 18/8/2021. Aditya Joshi. Kala Talav, Kalyan west.

Whitish layer in closed bottle after 48 hrs of fermentation. Aditya Joshi. Kala Talav Kalyan west. 18/8/2021
Only the open bottle shows bubbles?
Why the bubbles forms?

Bottle was swirled or shaken and then was tilted. The bus was dipped and then it was spread on potato slices. I used both hands. But to take picture, bottle was placed on ground n tilted.

18/8/2021. The fermented coconut water was spread on the boiled potato slices. Method: boiled potato slices were taken in a container with lid. A ear bud was used to take the fermented coconut water from the bottles. One bud for open bottle and one for closed bottle. The bud was dipped/soaked in the fermented coconut water. This bud was then spreaded on potato in S shape. Marked red are inoculated slices with open bottle coconut water. Marked blue are slices with closed bottle coconut water. Marked green are the non inoculated potato slices. Expectation: No colonies seen on non inoculated potato slices. Colonies should appear on inoculated slices. Date: 18/8/2021. Aditya Joshi. Kala Talav, Kalyan west.
[12:07 PM, 8/18/2021] Aditiya Joshi CUBE: I think these are the signs of fermentation
[12:08 PM, 8/18/2021] Aditiya Joshi CUBE: No. Some small bubbles were also in closed bottle.
Maybe these are of carbon dioxide released during fermentatiom
[12:08 PM, 8/18/2021] Aditiya Joshi CUBE: Fermentation*
Slices are kept in closed plastic box
See the point is that @Seethalakshmy CUBE that we are constantly trying explain the stuff that we are doing, in very basic and simpler terms and with examples and photos and references if possible.

Because people like me may not know what are clubed antenna . So it’ll get a hard way for me to get interested in your ants!!

What’s special about crazy ants!?
[1:30 PM, 8/18/2021] Abhijeet Singh: Great!!
I can see the legs of the ants are very shiny or whitish kind of. Is it because of the flash!?
[1:48 PM, 8/18/2021] Abhijeet Singh: We catch mosquitoes by clap trap method by making a hollow
in between both the hands, so that the mosquito is killed (but not smashed). We catch
only those mosquitoes that come to bite us in our house.

So this photo is from the presentation that we made for Cube National meet 2017 I guess(I’ll share that presentation shortly). We can see the white stripes easily on the Aedes mosquito with a black background. @Lydia cUBe Drosphila @Hina Mudgal Kanpur CUBE @KIRAN CUBE @Saida Sayyed @Aswathy Suresh CUBE
VIDEO SHARED BY SEETHALAKSHMI AT 1.59AM
Close up video of ants.
Location : Pazhuvil.
Time : 11:47pm.
Date:16/8/21.
Data collector : seethalakshmi CUBE
In this video ants have slightly blue colour​:roll_eyes:. May be due to flash light ??
This was our presentation in 2017 for mosquito project! @Arunan HBSCE @Hina Mudgal Kanpur CUBE @Lydia cUBe Drosphila @Saida Sayyed @KIRAN CUBE @Seethalakshmy CUBE @Aswathy Suresh CUBE @Batul Pipewala Kolkata
Please start with the objective so that readers’ curiosity is raised.
So do you think this is Nobel winning C elegans? On what basis? @Batul Pipewala Kolkata
So, what will be the approximate size? @Batul Pipewala Kolkata
Excellent
[2:42 PM, 8/18/2021] Arunan HBSCE: No Koel call these days, 3rd week of August, surely! Sorry, we should have been more particular to find out till when Koel calls were there in South Mumbai.
[2:43 PM, 8/18/2021] Arunan HBSCE: Some reference to your location like Chembur,Mumbai @DP HBCSE
Koel cooing
Location : Pazhuvil
Date:18/8/21
Time: 2:03pm
Data collector: seethalakshmi
[2:47 PM, 8/18/2021] Arunan HBSCE: Wow! More information like Authors, Year please @Rahul Kushwaha
[2:56 PM, 8/18/2021] Arunan HBSCE: Don’t you see worker ants carrying some white stuff? Are these not larvae or pupae?
Please look at your photos and tell us more about these.Wgat does references tell you?
[2:56 PM, 8/18/2021] Arunan HBSCE: @Seethalakshmy CUBE
[2:57 PM, 8/18/2021] Arunan HBSCE: Reference please! @Seethalakshmy CUBE
[2:59 PM, 8/18/2021] Arunan HBSCE: Please describe your major problem along with photos, so that readers can help you. Please spell it out. @Seethalakshmy CUBE
[3:01 PM, 8/18/2021] Arunan HBSCE: Don’t you have any reference link? Is it difficult to search for references? What key words you used? What search results you got? @Seethalakshmy CUBE please describe your worry.
[3:02 PM, 8/18/2021] Arunan HBSCE: Do you find it difficult to search for what is Imaginal disk. Please describe where you get stuck in this. @Seethalakshmy CUBE
[3:04 PM, 8/18/2021] Arunan HBSCE: Please give an example of a protein. @Seethalakshmy CUBE And explain how is it made up of @Seethalakshmy CUBE
Are there more nematodes in this slice than what you transferred? @Batul Pipewala Kolkata Please start with your objective with each such picture.
So, do you think C elegans. An be cultivated on raw potato slice with a few drops of milk? @Batul Pipewala Kolkata @Anshu Kadam (Nematodes)
What happened to the Controls, like without milk?@Batul Pipewala Kolkata
What is your objective? Always start with the objective to raise the curiosity of the reader @Aditiya Joshi CUBE What is your interpretation of the observation?

What is your further plan?@Aditiya Joshi CUBE
[3:13 PM, 8/18/2021] Arunan HBSCE: Please start with objective please! @Aditiya Joshi CUBE And what is your interpretation of your observation?
What is your further plan? @Aditiya Joshi CUBE
[3:16 PM, 8/18/2021] Arunan HBSCE: Why? Why should fermentation result in such indicators? @Aditiya Joshi CUBE
[3:17 PM, 8/18/2021] Arunan HBSCE: Why Carbon dioxide in fermentation? @Aditiya Joshi CUBE @Seethalakshmy CUBE
[3:18 PM, 8/18/2021] Arunan HBSCE: What is your expectation? @bunny~~ And why? @Seethalakshmy CUBE @Aditiya Joshi CUBE
[3:21 PM, 8/18/2021] Arunan HBSCE: Very important in Science and Science communication @Abhijeet Singh @Seethalakshmy CUBE Please help clarifying to get collaborators like @Abhijeet Singh
Wow! Please give more on it @Abhijeet Singh
Please start collecting mosquitoes in August till 23rd September when the Day and Night time will be equal, EQUINOX! @Abhijeet Singh @Hina Mudgal Kanpur CUBE @Seethalakshmy CUBE @Aswathy Suresh CUBE and others
No reference for measurement of size?!! @Seethalakshmy CUBE

Size of ant
Size of worker ant in my formicarium.
Size:2.8 mm
Location : Pazhuvil, Thrissur
Date:12/8/21
Time: 6:14
Data collector : seethalakshmi
@Arunan HBSCE sir
Please make dome specific comments @Seethalakshmy CUBE
What is striking in this compilation? @Seethalakshmy CUBE

What do you understand from Gaurangi’s and Ubaid’s findings? @Seethalakshmy CUBE

VIDEOS SHARED BY YASH AT 3.48PM
Today’s Headline - 11th Day update of SLPbA2 sub culture shows 50 fruit flies and few dead fruit flies!
Media in SlPbA2 is little liquidy as compared to SLPbB2 and C2!

Media date - 06/08/2021
Transfer date - 7/08/2021
No of flies transferred - 35

16/08/2021
No of flies -50
No of larvae - 5 - 3mm larvae
No of pupae - 20
No of dead flies - 10 @KIRAN CUBE maam @Arunan HBSCE sir @Aswathy Suresh CUBE @Sidhy Kerala Drosophila @Janhvi Deshmukh
SLPbB2

Today’s Headline - Today is the 11th day of Sub culture and SLPbB2 shows ~ 70 fruit flies with a few unhatched pupae.

Media date - 6/08/2021
Transfer date - 7/08/2021
No of fruit flies Transferred - 30

18/08/2021
No of flies - 70
No of larvae - - 4
No of pupae - 50
@KIRAN CUBE maam @Arunan HBSCE sir @Batul Pipewala Kolkata maam @Aswathy Suresh CUBE @Lydia cUBe Drosphila @Isha Pawle @Janhvi Deshmukh
SLPbC2 *Breaking News Record
Record of ~ 100 Fruit flies seen on the 11th Day of Sub culture in SLPbC2

Media date - 6/08/2021
Transfer date - 7/08/2021
No of fruit flies Transferred - 25

18/08/2021
No of flies - 100
No of larvae - - 8
No of pupae - ~ 20
No of dead flies - 10 @KIRAN CUBE maam @Arunan HBSCE sir @Batul Pipewala Kolkata maam @Aswathy Suresh CUBE @Sidhy Kerala Drosophila @Lydia cUBe Drosphila @Janhvi Deshmukh @Aastha Ahuja Cube
Breaking News Headline
SLPbA1 culture shows Zero flies, 0 pupae and larvae on 21st day!

Media prepared - 24/07/2021
Fruit fly transferred on - 24/07/2021 - One gramina fruit fly was transferred
18/08/2021
No of flies - 0
No of larvae - - 0
No of pupae - ~ 0
@KIRAN CUBE maam @Arunan HBSCE @Batul Pipewala Kolkata maam @Aswathy Suresh CUBE @Sidhy Kerala Drosophila @Lydia cUBe Drosphila @Janhvi Deshmukh @Aastha Ahuja Cube and others
Breaking News Headline
SLPbB1 culture shows 1fruit fly, 0 pupae and larvae on 21st day!

Media prepared - 24/07/2021
Fruit fly transferred on - 24/07/2021 - One gravid fruit fly was transferred
18/08/2021
No of flies - 1
No of larvae - - 0
No of pupae - ~ 0 @⁨Kiran Maam HBCSE⁩ maam @⁨Arunan Sir HBCSE⁩ sir @⁨Batul Ma’am CUBE⁩ maam @⁨Ashwathy CUBE Nattika⁩ @⁨Sidhy CUBE Nattika⁩ @⁨Isha HBCSE⁩ @⁨Lydia HBCSE⁩ @⁨Aastha⁩ @⁨Janhvi Deshmukh⁩ and others
Breaking News Headline
SLPbC1 culture shows 2fruit fly, 5 pupae and 0 larvae on 21st day!

Media prepared - 24/07/2021
Fruit fly transferred on - 24/07/2021 - One gramina fruit fly was transferred
18/08/2021
No of flies - 2
No of larvae - - 0
No of pupae - ~ 5

Summary of Fruit fly Cultures at CUBE Home lab Prabhadevi
SLPbA1 | SLPbB1 | SLPbC1 - Started on 24/07/2021
SLPbA2 | SLPbB2 | SLPbC2 - Started on 7/08/2021

Status of Cultures
Healthy Cultures - SLPb C2 +++
SLPbB2 - ++
SLPbA2 - +

+++ - Most Healthy culture showing plenty of Fruit flies, pupae and few larvae
++ -good culture - showing plenty of Fruit flies, pupae and less larvae

• • Reasonably good culture showing plenty of Fruit flies, few pupae and very less larvae

Bad cultures -SLPbB1 - ×××
SLPbA1 - ××
SLPbC1 - ×
××× - meaning - it Contains no Fruit flies, pupae and larvae with lot of fungal growth
×× - meaning - it Contains 1 or 2 Fruit flies, pupae and larvae with lot of fungal growth
× - Meaning it contains 1 or 2 Fruit flies and few pupae and no larvae with very less fungal growth
@Arunan HBSCE sir @KIRAN CUBEmaam @Batul Pipewala Kolkata maam @Aswathy Suresh CUBE @Lydia cUBe Drosphila @Sidhy Kerala Drosophila @Isha Pawle and others

VIDEO SHARED BY SIDHY AT 4.20PM
Single line culture of fruit flies.

TRSV medium - 15/08/2021, 11:00 PM
Transferred gravid fly on 16/08/2021 , 11:40 AM

3 Single line cultures : In the video , from left – SLTT A , SLTT B , SLTT C. ( SLTT - Single line culture Thanniam Thrissur)
Date of video : 16/08/2021
Location: Thanniam , Thrissur , Kerala.

Immediate objective : Make single line culture stocks of fruit flies.

Short term objectives :

1.Family level identification.
2. Conduct experiments based on observations and hypothesis.
3. Understand the life span of fruit fly.

Long term objectives :

1.Genus and Species level identification.
2. Olfactory sensitivity test.

Sidhy.P.P , CUBE S N College , Nattika.
Single line cultures of fruit flies.
SLTT A , B , C
Started on 16/08/2021
TRSV medium

18/08/2021
Observed ~ 20 small larvae in A and B , and ~ 10 small larvae in C.

Location: Thanniam , Thrissur , Kerala.
Sidhy.P.P , CUBE S N College, Nattika.
Do you think think this is Black Crazy ant? Why?@Seethalakshmy CUBE @Abhijeet Singh

S4E146 of CUBE chatShaala: Difference in the olfaction sensitivity of fruit flies

Webinar Link CUBE (tifr.res.in) Please click on the Link (BLUE) to join the CUBE ChatShaala Timing:5.30 pm to 9.30 pm Today’s session will be followed by a workshop on mosquito by 8pm onwards Kindly add your place/affiliation along with your name while joining Looking forward to your participation Thanks Please click on the below given link if you face any issue in joining the webinar

Kindly click on the above👆🏻 given link for the summary of last discussion.

Invitation to join today’s CUBE chatShaala,
Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in all CUBE what’s app groups and on metastudio after the session

Here’s the direct link of the CUBE webinar: https://webinar.hbcse.tifr.res.in/b/kir-hye-cn4
Note: we have changed the room link to avoid technical error

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
Congratulations to CUBE fruit fly Collaborators.

Sidhy has restarted single line Cultures
Congrats sidhy​:clap:
Kamakhya Temple reopens for devotees today amid COVID pandemic

Kamakhya Temple reopens for devotees today amid COVID pandemic

Source : “The Sentinel” via Dailyhunt

Download Now
http://dhunt.in/DWND
@Yash Sheregare CUBE all flies in SLPbA1 are died ??

PHOTO SHARED BY BATUL AND SEETHALAKSHMI AT 8.41AM
Update on Ant Model system…
They store sugars here.
Location : Pazhuvil.
Time : 6:44 pm.
Data collector : Seethalakshmi. C. R.

[KiranKalakotiR (Kiran Kalakoti)]

18th August 2021, 9.30pm to 19th August 2021, 9.30pm
Renamed the Cultures

In July 2020 , I had made 5 single line cultures. They were named as SLTT-A , SLTT-B, SLTT-C , SLTT-D and SLTT-E . After losing all these cultures , again started 2 new single line cultures in February 2021. To distinguish these cultures from 2020 cultures , named them as SLTT21-A and SLTT21- B. These two cultures lost in April 2021.

On 16 August 2021 , started 3 new single line cultures. And named them same as that of 2020 cultures. This will make confusions and affect the data of almost six months long single line cultures of 2020 which were also identified as of Drosophilidae family. I didn’t think this while naming. I thought that 2020 cultures are not existing and now I am starting the cultures after a gap of three and a half months.

In today’s chatshaala ,Arunan Sir asked me about this and made me understand the importance of nomenclature in research projects . Also I realised how valuable my old works and findings even when the cultures are not existing.

So , renamed all the single cultures of 2021 as a continuation of 2020 single line cultures. New names are :

SLTT - F ( SLTT21 - A) February
SLTT - G ( SLTT21 - B) February

SLTT - H 16 th August
SLTT - I 16 th August
SLTT - J 16 th August

@Arunan HBSCE Sir , @Lakshmy PJ CUBE , @KIRAN CUBE Ma’am , @Aswathy Suresh CUBE @Abhijeet Singh helped me in renaming and understanding the importance of nomenclature in projects. Thank you everyone.
Renamed the single line cultures of 16 th August 2021 as SLTT-H , SLTT-I , SLTT- J.

VIDEO SHARED BY SEETHALASHMI AT 11.54PM
Video of black crazy ants foraging on a sugar bait in Fiji, Vimeo video (© Eli Sarnat, Creative Commons Attribution, Share Alike CC BY-SA Licence)
I think my name is missing. Aditya Joshi, Kalyan, Maharashtra
Reference :

http://www.piat.org.nz/index.php?page=black-crazy-ant
This ant similar to my Formicarium Ant
Ok …I will add
VIDEO SHARED BY SEETHALASHMI AT 12.02AM
My formicarium ant. Location : Pazhuvil. Date: 13/8/21. Time: 12:04 am. Data collector : Seethalakshmi
Ants body shows slightly blue colour with flash light.
517 th day of CUBE ChatShaala: 18th August 2021.

19 CUBists from 19 Centres had joined the webinar.

INDIA

Delhi
Aastha Ahuja: ANDC
Dr P. Chitralekha: DSC

Haryana
Aashi: Panipat

Jharkhand
Morabadi, Ranchi: Rechel Tirkey

Kerala
Aswathy Suresh: Thriprayar
Lakshmy PJ: Kandassankadavu
Seethalakshmi CR: Pazhuvil, Trissur
Sidhy PP: Thanniam, Thrissur

Tamil Nadu
Meena Kumari: Chennai

Maharashtra
Aditya Joshi : Kalyan
Abhijeet Singh: Ulwe, Navi Mumbai
Arunan MC: Breach Candy, South Mumbai
Isha Pawle: Worli
Saida Sayyed: Bandra, Mumbai
Shraddha Sonavane: Panvel
Shivam Kumar Sriwas: Kalyan

Uttar Pradesh
Kiran Yadav: Moradabad
Aarti Vankatesan: Vigyanshaala
Divyanshi Chauhan: No…

Black crazy ants.
Thanks @Shraddha Sonavane CUBE

Iam just trying to take photos of my formicarium Ant. Location : Pazhuvil. Time : 2:20 pm. Date :30/5/21.Data collector : Seethalakshmi

Ant Life Cycle | Ask A Biologist

SUMMARY - CUBE Chatshaala.
18.08.2021

Single Line Culture updates @Sidhy from from Thanniam - Thrissur Kerala, Restarted fruit flies works on 16-08-2021 made new single line cultures.

There is an issue while naming the cultures.Because last year July 2020 also started 5 cultures named SLTT A, B, C, D & E. But unfortunately she lost those all culture after 6 months later due to some other reasons (Goof ups)

Then February 2021 again restarted 2 single line cultures named SLTT 21A, SLTT 21B that’s also lost after 2month later.

August 2021, made 3 new single line cultures started with one mother flies naming again same that she added in pervious single line culture bottles.

Why Naming Is Important!?

Importance of Nomenclature.

Whatever work we are doing or done in at particular model systems that all works were recorded in here or somewhere. So all details are valuable even names also important factors that will help to identify.

Aashi from Haryana were joined yesterday chatshaala.
She want to addressing about how to study microbes from various corner of home. Eg : microbes on fridge handle.

Is there any effects of sunlight in microbes.?

Difference between sunlight and some other lights, how will effects microbes!

Why only sunlight get effects microbes?

Culture microbes at homes!
How can we culture microbes at homes.

What type of media will help to grow microbes?

Why can’t use POTATO slice for to isolate microbes.

Why microbes growing very well on potato surface ? What could be the season?

Potato contains STARCH, Carbohydrates, Irons, Vitamins etc.

What is the factors that promoting microbes growth over potato slices?

STARCH

Starch is contains carbohydrates. Starch is a polymer of glucose C6 H12 O6. Complex Carbohydrates.

Importance Culture Media
Which type of Culture media selecting for to culturing microbes?

Aashi Batul Adithya Aastha please add your points
Microbes are present over the surface of potato slices Picture credit : Batul

@Aditiya Joshi CUBE Experiment isolating microbes from using coconut water.

Experiment details giving below

“18/8/2021. The fermented coconut water was spread on the boiled potato slices. Method: boiled potato slices were taken in a container with lid. A ear bud was used to take the fermented coconut water from the bottles. One bud for open bottle and one for closed bottle. The bud was dipped/soaked in the fermented coconut water. This bud was then spreaded on potato in S shape. Marked red are inoculated slices with open bottle coconut water. Marked blue are slices with closed bottle coconut water. Marked green are the non inoculated potato slices. Expectation: No colonies seen on non inoculated potato slices. Colonies should appear on inoculated slices. Date: 18/8/2021. Aditya Joshi. Kala Talav, Kalyan west.”

@Arunan HBSCE sir @Aditiya Joshi CUBE @Abhijeet Singh @Aashi @Batul Pipewala Kolkata @Aastha Ahuja Cube

VIDEOS SHARED BY ASWATHY AT 1.51AM
SLBK A22

Update : Fruit Flies
subculture started date : 12.08.2021

Transfer Flies : 1 Fly, Gravid one

Expectations :
18.08.2021 - 6th Day
~40 Eggs
~30 Larvae
~20 Pupae

Observation : 6th Day
18/08/2021
No of Eggs : ~6
No of Larvae : 0
No of Pupae : 0
No of Flies : 1 (Transferred one)

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
SLBK B21

Update : Fruit Flies
subculture started date : 12.08.2021

Transfer Flies : 12 Flies
Gravid Flies : 5, Male Flies : 7

Expectations : 6th Day
18.08.2021

~70 Eggs
~45 Larvae
~40 Pupae

Observation : 6th Day
18/08/2021
No of Eggs : ~30
No of Larvae : 5
No of Pupae : ~69
No of Flies : ~2 (Transferred flies)
Dead Flies : ~10

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
SLBK E17

Update : Fruit Flies
subculture started date : 12.08.2021

Transfer Flies : 14 Flies
Gravid Flies : 6, Male Flies : 8

Expectations :
18.08.2021 : 6th Day
~80 Eggs
~50 Larvae
~40 Pupae

Observation : 5th Day
18/08/2021
No of Eggs : 40
No of Larvae : 25
No of Pupae : 10
No of Flies : ~14 (Transferred flies)
Dead Flies : 0

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
SLBK E16

Update : Fruit Flies
subculture started date : 19.07.2021

Transfer Flies : 30 Flies
Gravid Flies : 9, Male Flies : 21

Expectations :
18.08.2021 : 31st Day
(One month old culture)
~150 Eggs
~80 Larvae
~160 Pupae

Observation : 31Day
18/08/2021
No of Eggs : ~150+
No of Larvae : ~20
No of Pupae : ~170+
No of Unhatched Pupae : ~60+
No of Flies : ~120+
Dead Flies : 40+

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
BREAKING NEWS
SLBK E15 - 47 Days old subculture bottle have ~100 Eggs, ~160 Pupae, ~30 Flies, Media still solidified condition.

SLBK E15

Update : Fruit Flies
subculture started date : 02.07.2021

Transfer Flies : 15 Flies
Gravid Flies : 8, Male Flies : 7

Expectations :
18.08.2021 : 47 Days old culture bottle
~200 Eggs
~80 Larvae
~50 Pupae

Observation : 47 Day
18/08/2021
No of Eggs : ~100
No of Larvae : ~8
No of Pupae : ~160
No of Unhatched Pupae : ~60
No of Flies : ~30
Dead Flies : ~60

Media solidified

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur.
[9:22 AM, 8/19/2021] Batul Pipewala Kolkata: Wow!
[9:23 AM, 8/19/2021] Batul Pipewala Kolkata: There are 100s of nematodes in this culture which was started with 2to 3 nematodes
[9:24 AM, 8/19/2021] Batul Pipewala Kolkata: Yes C elegans can be cultured on raw potato slice with a few drops of milk added on the first day
[9:25 AM, 8/19/2021] Batul Pipewala Kolkata: Approximate size of the nematodes is 1.5 to 2mm
[9:26 AM, 8/19/2021] Batul Pipewala Kolkata: No this is not C elegans because the middle bulb is not completely round as given in references

Cardamine species at CUBE HomeLab, Bhulabhai Desai Road, South Mumbai 19, August 2021 Photo: mcarunan let’s follow up with data from collaborators from all CUBE centers.
A close relative of the model system, Arabidopsis for plant sciences research.https://www.ceplas.eu/en/research/principal-investigators/prof-dr-miltos-tsiantis/

I think a fruit fly visited my setup to taste toddy… Open bottle of fermenting coconut water. 19/8/2021. Kala Talav, Kalyan west.

3rd day of coconut water fermentation… We can see a whitish layer settled at the bottom of bottles. The layer gets disturbed when swirled. A thin white layer is present on surface of coconut water too. 19/8/2021. Kala Talav, kalyan west.
Phyllanthus growing well in the pots. Kala Talav, Kalyan west.
This phyllanthus in pot2 have produced many branches. Its flowering too… But not producing a fruit. What can be the reason🤔 @Lakshmy PJ CUBE @Sunita Chahar CUBE @Shraddha Sonavane CUBE @Seethalakshmy CUBE @Arunan HBSCE @P.chitralekha and all others. Kala Talav, Kalyan west. 19/8/2021.

VIDEO SHARED BY ARUNAN AT 10.59AM
Date:5/08/21.Time :11 31am.seed bursting in cardamine. A small touch is enough to break the matured seed pods of cardamine. Arunima CUBE Home lab Kozhikode Kerala.
[11:50 AM, 8/19/2021] Aswathy Suresh CUBE: Larvae that I found in culture after 6th day. Most of the larvae I observed it was 3rd instar stage.1st instar & 2nd instar larvae couldn’t able to find out inside culture.

First pupae observed in E culture on 6th day, ~10 pupae formed inside culture on 18/08/2021.

Observations Based Analysis :

12.08.2021 - Transferred flies 14 flies, 6 gravid flies, 8 male flies.

13.08.2021 - No eggs.

14.08.2021 - No eggs, No Larvae observed.

15.08.2021 - No eggs, No Larvae observed.

16.08.2021 - Eggs ~10 , No Larvae, No Pupae observation.

17.08.2021 - Eggs ~30 , No Larvae, No Pupae

18.08.2021 - Eggs ~80 , 45 more larvae (Different stage) mostof them observed below the media side, 10 Pupae.
[11:55 AM, 8/19/2021] Aswathy Suresh CUBE: Expecting eggs inside the A22 culture. Because I transferred only one fly that reminded in A21 culture. Look like it is gravid one that’s why Iam expecting some eggs will laying that one fly inside in A22 culture.
[11:58 AM, 8/19/2021] Aswathy Suresh CUBE: Yes sir, I missed larvae inside E16 culture bottles. Because I am not much expected any larvae has remained after 1 month old bottles.
[11:59 AM, 8/19/2021] Aswathy Suresh CUBE: Collaboration through helping find out things that we can’t able see it but others can.
[11:59 AM, 8/19/2021] Arunan HBSCE: What is your expectations now, from tomorrow onward, in this bottle? That will help you to understand what is good or bad about your your culture conditions of Single Line E flies which were in very good shape so far.
Don’t you think so? @Aswathy Suresh CUBE
What are your comments @Yash Sheregare CUBE @Lydia cUBe Drosphila @Aastha Ahuja Cube @Batul Pipewala Kolkata and others
[12:01 PM, 8/19/2021] Arunan HBSCE: Why what is the basis for that? Is a culture of one month is too old? @Aswathy Suresh CUBE
What evidence we gave for that?
[12:04 PM, 8/19/2021] Arunan HBSCE: What is the best time (after how many days) to transfer flies for subculture from old bottles to fresh culture bottles? @Aswathy Suresh CUBE @Yash Sheregare CUBE @Aastha Ahuja Cube
[12:06 PM, 8/19/2021] Arunan HBSCE: if flies are thriving in
such bottles, don’t they mate and lay eggs and the eggs hatch to larvae? What makes you think that there can it be larvae? @Aswathy Suresh CUBE @Yash Sheregare CUBE
Is this not a nice Goof- up to celebrate so thate every one realizes that goof ups are common when we do research.
And such realization very important for progress in science! @Aswathy Suresh CUBE @Lydia cUBe Drosphila @Yash Sheregare CUBE @KIRAN CUBE
Expectations about E17 culture.

Expectations today onwards :

19.08.2021 - ~100 eggs + ~60 Larvae + ~25 Pupae

20.08.2021 - ~150 eggs + ~70 Larvae + ~30 Pupae

21.08.2021 - ~200 eggs + ~85 Larvae + ~50 Pupae + ~10 New flies + ~14 Old flies.
Monsoon plants… Raanbhendi/(wild okra)… Beautiful yellow flowers… Often seen growing in monsoon along with roadsides, gardens, backyards, railway tracks… The flowers, buds n small tender okra can also be seen here. Kala Talav, Kalyan west. 19/8/2021. Ate these seen in other parts of mumbai n India…? Lets find…
[12:23 PM, 8/19/2021] Arunan HBSCE: Sorry, without other essential information like the Date of Transfer into SLBT E17 bottles, this write up does not communicate much @Aswathy Suresh CUBE
Please repost so that others will be inspired to comment and become collaborators.
Please repost essential information. @Aswathy Suresh CUBE
[12:25 PM, 8/19/2021] Arunan HBSCE: Do you have larvae, pupae and adults today, 19 August 2021, in this bottle?@Aswathy Suresh CUBE
Please post evidence too.@Aswathy Suresh CUBE
Photo with plane white background.

Sir, I think this image will more better when shape of leaflets are clearly visible.
It will be help in identification of Cardamine
Ohh!! Toddy lover Fruit fly😋
Wow!!! @Aditiya Joshi CUBE Your phyllanthus plants are grown up.
Great @Aditiya Joshi CUBE
What is your next plan?
Can you please show the below part of the branches.There may be fruits produced. Later they fall off @Aditiya Joshi CUBE
[12:33 PM, 8/19/2021] Arunan HBSCE: @Abhijeet Singh Please continue commenting on a posts.
[12:36 PM, 8/19/2021] Arunan HBSCE: on all*
What about other plants?
Are they fruiting?
[12:51 PM, 8/19/2021] Aswathy Suresh CUBE: Not much old culture bottle is that.it Is just passed one month. Most of my observation after around one months bottles have less number of larvae. That’s why I mentioned I like that.
[12:51 PM, 8/19/2021] Aswathy Suresh CUBE: 20-25 is the Best time for to transfer flies in to new culture media bottles.
20-25 Days in normal situation (Good number of Larvae, Pupae, Adult flies).

But is there any emergency situation time come, don’t wait for that much time. immediately need to transfer those flies in new media bottle in order to save those flies.

PHOTO SHARED BY LAKHSMI AT 12.58PM
Long term objective -Floral dip method
Short term objective -

1. To observe life cycle
2. observing heteroblasty(To observe the transformation from simple to compound leaves.
   3)Identifying cardamine plants

Date:16/8/2021
Time:5:38pm
Place: Mampilly
Cotyledonary stage+ 4 leaf stage.
All are simple leaves

Phyllanthus urinaria mature fruits collected on 15/8/21
date of photo–19/08/21. Objective- To study Seed germination in Phyllanthus plant.
location- Panvel Photo taken by- Shraddha
Date:16/8/2021
Time:5:38pm
Place: Mampilly
[1:01 PM, 8/19/2021] Aditiya Joshi CUBE: I think my fruits fall off… Only some parts are seen fallen in soil…
[1:02 PM, 8/19/2021] Aditiya Joshi CUBE: Yes. Others plants in pot3 are fruiting
Please let us know how many fruitflies you have now! From all the cultures that are doing good!

I think SlPb A2/B2/C2 are doing good!

What’s your further plan @Yash Sheregare CUBE !!!
Please post updates of all culture bottles of SLBK E to show what is the right type of culture and what is not good culture and why @Aswathy Suresh CUBE
Let’s all get lessons from this. @Aswathy Suresh CUBE
Great ! @Shraddha Sonavane CUBE How many seeds in each fruit?
Yes @Lakshmy PJ CUBE I’ll post photos. I have seen flowers falling off
So @Sidhy Kerala Drosophila how are your cultures doing in order to achieve your Immediate term objective!?
How would you comment on it?
Let us know what are the difficulty you are facing now! Let’s discuss it out!
@Aswathy Suresh CUBE @Lydia cUBe Drosphila @Yash Sheregare CUBE
BREAKING NEWS SIMPLE TO COMPOUND LEAF TRANSFORMATION.Date:18/8/2021
Time:5:23pm
Place: Mampilly
Cotyledonary stage+ 4 leaf stage
The 4th simple leaf split in to compound leaf

White shiny thread like structure observed on dry stem of Phyllanthus urinaria plant. What can it be? Date of photo-19/08/21 location-Panvel data collected by-Shraddha @Aditiya Joshi CUBE @Arunan HBSCE Sir @Batul Pipewala Kolkata mam @Aswathy Suresh CUBE @Seethalakshmy CUBE @Aashi & others…
Yess that would be great!
Let’s start it doing together!
@Aswathy Suresh CUBE @Hina Mudgal Kanpur CUBE @Lakshmy PJ CUBE @Seethalakshmy CUBE
Please comment and let’s plan!!
[1:12 PM, 8/19/2021] Aditiya Joshi CUBE: Same @Shraddha Sonavane CUBE
[1:12 PM, 8/19/2021] Aditiya Joshi CUBE: I’ll post photos
Date:19/8/2021
Time:1:11pm
Place: Mampilly
Cotyledonary stage+ 4 leaf stage
The 4th simple leaf split in to compound leaf
5th leaf emerging
Date:19/8/2021
Time:1:11pm
Place: Mampilly
Cardamine plants growing healthy.
Looks like fungi to me

White thread like growth on dried stems… Kala talav, Kalyan west.@Shraddha Sonavane CUBE

White thread like growth seen on dried stems.
19/8/2021
I hope in this photo white shiny threads are clearly visible.& I can see some yellow spores in orange colour circle
Flowers have fallen fron the leaves @Lakshmy PJ CUBE @Shraddha Sonavane CUBE
Yes…it’s similar

Dried flowers underside of leaves. Pot2 phyllanthus.
Wow!! This video is so amazing! We can see the white stripes on the back of ants.
Please comment on how do we stand to our claim that the ants we have are Black Crazy Ants too! @Seethalakshmy CUBE
Wow great @Shraddha Sonavane CUBE Does these fruits burst out?
Please keep it in closed container. Otherwise they will burst out and disperse seeds.
Yes I think that they look similar from the once you sent before!
I think 3 seeds in single capsule
Is that root hairs @Shraddha Sonavane CUBE
[1:26 PM, 8/19/2021] Lakshmy PJ CUBE: Which means they are fungus? @Shraddha Sonavane CUBE
[1:26 PM, 8/19/2021] Lakshmy PJ CUBE: @Aditiya Joshi CUBE
[1:26 PM, 8/19/2021] Shraddha Sonavane CUBE: Most of fruits are turned brown but not burst out.

Yes, kept it in closed container
[1:27 PM, 8/19/2021] Shraddha Sonavane CUBE: No @Lakshmy PJ CUBE .
There are on stem
Is it because of any Pest attack @Aditiya Joshi CUBE
[1:28 PM, 8/19/2021] Shraddha Sonavane CUBE: & on branches
[1:29 PM, 8/19/2021] Shraddha Sonavane CUBE: I don’t have any idea. I have to check
[1:31 PM, 8/19/2021] Shraddha Sonavane CUBE: Mam, is it because of white shiny threads like appearance?
Actually no insect or pest are seen on plant. Its producing new branches n sprouting new leaves… Flowers too… But no fruiting yet…
Can it be a mealybug infestation? @Shraddha Sonavane CUBE @Lakshmy PJ CUBE @Aditiya Joshi CUBE
I too had observed similar white structures on the Phyllanthus plant that I had brought from my building compound. And the leaves too had wilted out…that resulted in drying up of the whole plant.
Wooooooo A22 Bottle
That’s amazing this is your 23rd Bottle of A series. @Aswathy Suresh CUBE you have been doing a wonderful efforts.

But why this bottle is not fulfilling your expectations?
Wooow you got good number of Pupae but why flies are drying.

10 dead flies in 6 days
But there is no mealy bug attack on my Phyllanthus plants yet.
Nice photo with a nice background.

Is this an invasive species or local Cardamine of Mumbai?

What species of Cardamine this could be?
@Shraddha Sonavane CUBE @Arunima SN Nattika Cardamine @Lakshmy PJ CUBE @Batul Pipewala Kolkata and other Cardamine collaborators.
How do we indentify the species of Cardamine?
I think we should check… it can be a possibility of fungi … because fungi can grown on dry & decay matter too.
What you think @Aditiya Joshi CUBE @Lakshmy PJ CUBE @Drishtant Cube Moina
Another Cardamine from another place of India.

Is this the munnar variety? Or plant brought from local plant nursery?

Which species of Cardamine this could be?
Wooowww another Cardamine but from which place of India?
@Lakshmy PJ CUBE did you find them in your surroundings or you got these Cardamine from plant nursery?
What is the expectation? When do you expect to observe floral buds in these Cardamine plants?
If this is the case, then did @Aditiya Joshi CUBE observe the white structures on dried up plants or green, healthy Phyllanthus plants?
In how many days after the seed germination does this compound leaf has arises? What do you mean by transformation? Was this leave simple before?
Dried stems… dead plants @Drishtant Cube Moina
So, let’s rule out either of the possibilities that we have thought of; Fungi or mealybugs?
What do Fungi normally need to grow/sustain? The same question goes for mealybugs.
Any more inputs to add to the protagonists?
Absolutely sir!

PHOTOS SHARED BY ABHIJEET AT 2.16PM
Fungus going on a wooden block in my balcony. Amazing site!! Date:- 19/08/2021, Time: 2.15pm, Location:- Ulwe, Navi Mumbai, Maharashtra.
Green once are:- Trichoderma Trichoderma - Wikipedia … Google lence application suggests! Date:- 17/08/2021, Time:- 2.15pm, Location:- Ulwe, Navi Mumbai, Maharashtra.
White once are :- Crepidotus https://en.m.wikipedia.org/wiki/Crepidotus Time:- 2.15pm DATE:- 19/08/2021. Location:- Ulwe, Navi Mumbai, Maharashtra.

VIDEOS SHARED BY ASWATHY AT 2.17AM
SLBK A 22 - single line sub culture bottle of A Line… Date of transfer flies 12.08.2021.

Expectations on 7th Day
19/08/2021
~50 Eggs
~40 Larvae
~30 Pupae

Observations on 7th Day
19/08/2021
~40 Eggs
~ 2 Larvae
~ 0 Pupae
1 Flies (Transferred)

Update : Aswathy Suresh CUBE SN College Nattika.
Home Lab : Bottukadavu - Thrissur Kerala.
SLBK B21 - single line sub culture bottle of B Line… Date of transfer flies 12.08.2021.

Expectations on 7 Day
19/08/2021
~80 Eggs
~55 Larvae
~50 Pupae

Observation : 8th Day
19/08/2021
No of Eggs : ~50
No of Larvae : ~10
No of Pupae : ~70
No of Flies : ~7
Dead Flies : ~10

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur Kerala
SLBK E17 - single line sub culture bottle of E Line… Date of transfer flies 12.08.2021.

Expectations on 7 Day
19/08/2021
~80 Eggs
~55 Larvae
~50 Pupae

Observation : 8th Day
19/08/2021
No of Eggs : ~60
No of Larvae : ~30
No of Pupae : ~72
No of Flies : ~14
Dead Flies : ~0

Update : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur Kerala

Pre - Pupae (Larvae turned in Pupae Condition) Picture taken from SLBK E17 subculture bottle.
I am sure there must be more larvae with such number of adult flies! @Yash Sheregare CUBE
Don’t you expect? @Aswathy Suresh CUBE @Aastha Ahuja Cube @Abhijeet Singh
Any explanation? @Yash Sheregare CUBE @Aswathy Suresh CUBE
When did you last see any larvae, pupae or adult fly in this bottle? @Yash Sheregare CUBE
Excellent summary @Yash Sheregare CUBE
Excellent write up! Please give a BREAKING NEWS? @Sidhy Kerala Drosophila
No fly? And when do you expect Pupae? @Sidhy Kerala Drosophila
How do you describe the features of these ants? @Seethalakshmy CUBE
Why not take photos along with some reference Scale? @Seethalakshmy CUBE
[3:33 PM, 8/19/2021] Arunan HBSCE: Photo of eggs please ! @Aswathy Suresh CUBE
[3:44 PM, 8/19/2021] Arunan HBSCE: Middle bulb? Is it how this structure is described in references? Please give reference. @Batul Pipewala Kolkata
What causes the beginning of compound leaves in Cardamine? @Lakshmy PJ CUBE @Arunima SN Nattika Cardamine
Any differential expression of genes implicated for starting the development of Compound leaf from Simple Leaf state? @Lakshmy PJ CUBE @Arunima SN Nattika Cardamine @KIRAN CUBE @P. Chitralekha Cube JNU @Shyam T.G Cube SN Nattika Kerala
[3:53 PM, 8/19/2021] Arunan HBSCE: Any new gene expression? @Lakshmy PJ CUBE
[3:55 PM, 8/19/2021] Arunan HBSCE: Any reference? @Shraddha Sonavane CUBE
[3:57 PM, 8/19/2021] Arunan HBSCE: @Arunima SN Nattika Cardamine Please respond.

S4E149 CUBE chatShaala: How to name and label experimental set ups?

Yesterday in the session we had the context to understand how to name and label experimental set ups. @sidhypp one of the fruit fly CUBE collaborators has restarted the single line cultures of fruit flies all over again but the name of these new bottles are same as previous ones. CUBE ROOM LINK Please click on the CUBE (BLUE) to join the CUBE ChatShaala Timing:5.30 pm to 9.30 pm Kindly add your place/affiliation along with your name while joining. Please click on the below given link if yo…

Kindly click on the above👆🏻 given link for the summary of last discussion.

Invitation to join today’s CUBE chatShaala,
Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in all CUBE what’s app groups and on metastudio after the session

Here’s the direct link of the CUBE webinar: https://webinar.hbcse.tifr.res.in/b/kir-hye-cn4
Note: we have changed the link to avoid technical error

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.

Pseudomelanism.

In a world first case ,this zebra , born in Kenya’s Mara Reserve with unique polka dot appearance

A rare genetic mutation in which animals display some sort of abnormality in their stripe pattern,zebra foal with a dark coat and white polka dots has been spotted in Kenya’s Masai Mara National Reserve.
Data taken from : National geographic 2/9/2019.
Data collected by: Theertha
CUBE SN Nattika.

Genetic change results in unusual zebra foal - Genome BC

VIDERO SHARED BY MAN AT 6.27AM
CUBE Ranchi ReMan’s Home Lab 19-08-2021
Fruitfly Model System Update

Immediate Objective: to start a single line fruitfly culture(RNSL-B) at ReMan’s Lab in TRSV Media

Short Term Objective: -To identify the trapped fruitflies
-To get native Drosophila melanogaster species

Media prepared- 18-08-2021
Transferred date- 19-08-2021

Activity
-Transferred a gravid fly in a TRSV media bottle, 24 hours after media preparation
-Tagged the bottle as RNSL-B

Expectation
-New flies will emerge on 27th Aug, 2021
Analysis of circadian leaf movement rhythms in Arabidopsis thaliana - PubMed.

Analysis of circadian leaf movement rhythms in Arabidopsis thaliana
Great @Man Masih Beck RANCHI CUBE @Rechel Turkey Cube Ranchi Moina
When did you started this single line culture ?
How many single line cultures you made?
What is your objective?
@Man Masih Beck RANCHI CUBE @Rechel Turkey Cube Ranchi Moina
I transferred one gravid fruitfly in TRSV media bottle to prepare one single line (RNSL-B).
The immediate objective is to get successful in maintaining the culture as well as observe it’s lifecycle.
Cardamine with caterpillar 13 February 2020

13 January 2020 How Shraddha Sonawane story of Host specificity of butterfly

7 Feb2020 HBCSE, TIFR Butterfly & Host plant studies, looks like.

[KiranKalakotiR (Kiran Kalakoti)]

19th August 2021, 9.30pm to 21st August 2021, 9.30pm

19 CUBists from 19 Centres had joined the webinar.

INDIA
Delhi
Aastha Ahuja: ANDC
Dr P. Chitralekha: DSC

Haryana
Aashi: Panipat

Jharkhand
Rechel Tirkey: Morabadi, Ranchi

Kerala
Aswathy Suresh: Thriprayar
Lakshmy PJ: Kandassankadavu
Seethalakshmi CR: Pazhuvil, Thrissur

Maharashtra
Aashutosh Mule: Ghatkopar, Mumbai
Abhijeet Singh: Ulwe, Navi Mumbai
Aditya Joshi: Kalyan
Arunan MC: Breach Candy, South Mumbai
Mandar Chavan : Andheri, Mumbai
Saee Kamat: Fergusson, Pune
Shraddha Sonavane: Panvel

Uttar Pradesh
Kiran Yadav: Moradabad
Kanchan Bafila: Pithoragarh

West Bengal
Batul Pipewala: Kolkata

Meena
Prachi Pant

Note
Cubist(s) who had joined today but their name isn’t included in the list, kindly replied to this message.
After 4th Compound leaf, First floral buds was observed. date of photo-31 Jan 2020. location- G6 lab, CUBE HBCSE,Mumbai. Photo taken by- Shraddha Sonavane
The crazy ant worker is relatively small (2.3-3 mm). The head, thorax, petiole, and gaster are dark brown to blackish (Creighton, 1950); the body often has faint bluish iridescence. The body has long, coarse, scattered, suberect to erect, grayish or whitish setae (hair-like projections).

https://entnemdept.ufl.edu/creatures/urban/ants/crazy_ant.htm
Yes sir, The reference ( published by orkin canada) says this

The larvae then shed their skin to become pupae, which look like white adult ants but with their legs and antennae folded up against their body.

Ants | Facts & Identification, Control & Prevention

ants but with their legs and antennae folded up against their body.

Ants | Facts & Identification, Control & Prevention

Meet the BLACK CRAZY ANTS (Paratrechina longicornis) │ Pest or Neighbors?

@Arunan HBSCE, @Man Masih Beck RANCHI CUBE, @Batul Pipewala Kolkata ma’am, @KIRAN CUBE ma’am, @Aswathy Suresh CUBE, @Lakshmy PJ CUBE, @Shraddha Sonavane CUBE, @Shraddha Sonavane CUBE, @Abhijeet Singh, @Vishnupriya CUBE

VIDEO SHARED BY SEETHALAKSHMI AT 1.02AM
Iam observed a big ants in their colony.
Location : Pazhuvil.
Time :11:40 pm.
Date:16/8/21
Data collector : seethalakshmi
[1:06 AM, 8/20/2021] Seethalakshmy CUBE: More videos about it
Loction : pazhuvil
Date:16/8/21
Time:11:40 pm
[1:11 AM, 8/20/2021] Seethalakshmy CUBE: Yes, first we try to find the breeding places of mosquitoes
[1:14 AM, 8/20/2021] Seethalakshmy CUBE: Yes This my major problem
How can check it’s morphology?
Is it possible by using mobile microscopy
[1:16 AM, 8/20/2021] Seethalakshmy CUBE: Yes, we are know but we need scientific evidence
Including their morphology.
That is essential for convincing others
[1:18 AM, 8/20/2021] Seethalakshmy CUBE: That’s very essential for my ants study
Please help me plan this
How can check its morphology???
@Batul Pipewala Kolkata ma’am @Arunan HBSCE sir, @KIRAN CUBE ma’am, @Aditiya Joshi CUBE, @Lakshmy PJ CUBE, @Aswathy Suresh CUBE, @Abhijeet Singh, @Man Masih Beck RANCHI CUBE
And others
[1:19 AM, 8/20/2021] Seethalakshmy CUBE: Ok sir. I will take
[1:29 AM, 8/20/2021] Seethalakshmy CUBE: Black crazy ants
Kingdom:
Animalia
Phylum:
Arthropoda
Class:
Insecta
Order:
Hymenoptera
Family:
Formicidae

Body division : head, Thorax abdomen
Long - antenna and long 6 six legs.
Its common name arises from its characteristic erratic and rapid movement, and habit of not following trails as often as other ants
Habitat
Nesting : damaged trees, soil, indoors
Food: Sugar, sweety substances, insects
Colour : brownish black
Size of workers :2.3-3mm
Non - clubbed antenna ( hair like )

How to introduce model systems in a simple way that every people can understand, get inspired and do some works on it. Specially who are join in Chai & Why section, even non biologists.

Today we were discussed about model systems.
Phyllanthus
Nematodes

But we covered other areas like Butterfly - Plant interaction, Cardamine (Gene expression).

@Lakshmy PJ CUBE @Shraddha Sonavane CUBE @Aditiya Joshi CUBE mentioned about Journey about when did started doing work in CUBE.

Story of Butterfly’s studies done in HBCSE Campus (Butterfly - Plants interaction study done by cubits @KIRAN CUBE @Yash Sheregare CUBE @Drishtant Cube Moina @Lydia cUBe Drosphila …& others)

Psyche butterfly Larvae & Eggs were found in cardamine plants.

7 Feb2020 HBCSE, TIFR Butterfly & Host plant studies, looks like.

Cardamine Leaves stage study
Research Q : Floral dip method in Cardamine flowering bud.
Which leaves stage cardamine flowering bud will form in order to study floral dip method.
4-6 stages of leaves were showing flowering. Total 45 to 50 days taken for to get first floral bud in cardamine plants.

Gene Expression in Cardamine plants.
KNOX Genes : Compound leaves formation because of KNOX genes expression.
Simple leaves change in to compound leaves.

One of other Gene expression example is Moina. Body color change in White to Red. This condition called Hypoxia Condition. Happen in low oxygen levels (Unfavorable conditions)

What are the exact process of gene expression in cells ?

Before that we need to know what is Gene? Where can gene present inside nucleus!.
Tryingto find out this kind of fundamental questions answers through communication.!
Yesterday Discussion Based Reference Given in CHATBOX.

Analysis of circadian leaf movement rhythms in Arabidopsis thaliana Arabidopsis thaliana is the model organism for the study of the higher plant circadian clock. The physiological change in position of young leaves and cotyledons in Arabidopsis seedlings reveals an overt circadian rhythm.
Analysis of circadian leaf movement rhythms in Arabidopsis thaliana - PubMed.

Although Arabidopsis lacks the pulvinus, it displays rhythms in cell elongation and, thus, in growth rate. For example, there is a circadian rhythm in the elongation rate of the abaxial and adaxial cells of the petiole that confers an oscillation in position of cotyledons and leaves (Engelmann and Johnsson, (1998)).circadian rhythm Arabidopsis - Google Search

Analysis of circadian leaf movement rhythms in Arabidopsis thaliana - PubMed.
Analysis of circadian leaf movement rhythms in Arabidopsis thaliana

Life History of the Psyche

https://www.semanticscholar.org/paper/KNOX-genes%3A-versatile-regulators-of-plant-and-Hay-Tsiantis/3a4eaa60fa0951d978689e116fbb1dd4004738cf/figure/7
Interesting picture on Knox gene

A developmental framework for dissected leaf formation in the Arabidopsis relative Cardamine hirsutashow that lateral leaflet formation in C. hirsuta requires the establishment of growth foci that form after leaf initiation. A developmental framework for dissected leaf formation in the Arabidopsis relative Cardamine hirsuta - PubMed

Class I KNOTTED1-like homeobox (KNOX) proteins also promote leaflet initiation in C. hirsuta, and here we provide evidence that this action of KNOX proteins is contingent on the ability to organize auxin maxima via the PINFORMED1 (PIN1) auxin efflux transporter
Summary of CUBE Chatshaala (19/8/21)
We started discussion on Chai & Why session.
✓What is Chai & Why?
✓ Introduction of Chai & Why.
✓How to introduce all model systems.

So we started discussion on C.elegans & Phyllanthus.

In C.elegans model organism - we discussed how to connect Round worm in stomach with Nobel prize winning work & with Isolating of C.elegans on potato slices.

In Phyllanthus model organism - we discussed about how to connect Nobel prize winning work in fruit flies with Continuous light & dark experiment in Phyllanthus. also leaf angle titration connect with acid base titration.
Also highlighted on Phyllanthus niruri controversy.
[2:15 AM, 8/20/2021] Shraddha Sonavane CUBE: Today’s discussion was on 4 different plants -
1.Mimosa
2. Phyllanthus
3.Cardamine
4.Mango mapping

In Phyllanthus we discussed about
@Lakshmy PJ CUBE’s story of Phyllanthus-
Initially @Lakshmy PJ CUBE was working on Mimosa plants for Circadian rhythm study.she also done seed germination study in Mimosa plant.
Then she working on Phyllanthus for Circadian rhythm study, Identification of Phyllanthus species,leaf angle titration.
Collaborators- @P. Chitralekha Cube JNU mam @Nidhi @Anushka Datta @ Anulekha @Shraddha

Then we discussed on my (Shraddha) story of Phyllanthus. I found some plants in plants pot at home.
I got interested because of Circadian rhythm study was in my MSc syllabus & because of observations done by @Harshad sir,in CUBE HBCSE.
[2:15 AM, 8/20/2021] Shraddha Sonavane CUBE: Great observations done by Susanta Tanti Sir & their students while returning to Assam from Mumbai by train.
They were observed how many Mango trees was flowering & then they mapped it.

Then we discussed on Cardamine plant-

• Lifecycle of Psyche butterflies on Cardamine plant were observed in G6 lab HBCSE.

• how many days required to get first flower buds?

• after how many Compound leaves first flower buds arose?

• why Cardamine having compound leaves & Arabidopsis having simple leaves?

Which genes responsible for leaf diversity in both?

•how do gene expressed?so we compare Cardamine- plant model organism with Moina

While understanding gene expression questions was arise-
1.what is a gene?
2.where it’s located inside the cell?
3.how gene expressed?
4.why Chromosomes are important in cell?
5.what is Chromosomes & Chromatin?
@Seethalakshmy CUBE @Aswathy Suresh CUBE @Aditiya Joshi CUBE @Lakshmy PJ CUBE @Aastha Ahuja Cube @Mandar
It’s a caradamine? @Lakshmy PJ CUBE @Drishtant Cube Moina @Arunan HBSCE sir @Aditiya Joshi CUBE and others
How to identify caradamine ?
I think its oxalis… M not sure. But I thik its oxalis… This also grows as a weed in lawn, backyards, pots, gardens… @Janhvi Deshmukh
Yes I see in my area near the tulsi @Aditiya Joshi CUBE

Oxalis
oxalis
Ohh @P. Chitralekha Cube JNU mam thanks mam
[10:13 AM, 8/20/2021] Arunan HBSCE: What is the question? @Shraddha Sonavane CUBE
[10:17 AM, 8/20/2021] Arunan HBSCE: Don’t you think photos of some of your ants which you call as Black Crazy Ants in which the abdomen of the ants show prominent light band? @Seethalakshmy CUBE
Do you have any current data of Mosquitoes of Mumbai or Navimumbai? @Abhijeet Singh
What is your interpretation @Batul Pipewala Kolkata when compared with the experience of @Aswathy Suresh CUBE
One of the nematodes is much larger than the other. Any explanation? @Batul Pipewala Kolkata
Wt is the name of this nematode?
Are you convinced these are nematodes? @Ankit Yadav Xms Work Shop Cube Patkar College
Which ants are these? @Seethalakshmy CUBE please start describing from here.
What is the summary, so far, on your CUBE HomeLab for Fruity Model system? @Yash Sheregare CUBE
Yes sir. They hv all features of nematodes
What is the summary, so far, on your CUBE HomeLab for Fruity Model system? @Sidhy Kerala Drosophila
“here” means where? Please describe. What is your interpretation? @Seethalakshmy CUBE

Which Ants are these?@Seethalakshmy CUBE
Don’t you think the ants in your fomicarium looks different from these? @Seethalakshmy CUBE
Look, how this video maker use a reference scale, so well.
Great! @Seethalakshmy CUBE How many formicarium do you have in your CUBE HomeLab Pazhuvil? @Seethalakshmy CUBE
@Sidhy Kerala Drosophila @Aswathy Suresh CUBE
Maintaining log book is one of the essential part in research but CUBE always does simple things.

Why not to give update on your single line cultures and this update will be archive in this context 2 Curriculum group.

A very simple way to maintain a log book: oopps😇 e-log book (send some good photos and videos along with minimum essential write up) by sending daily update on CUBE WHAT’S APP GROUPS

@Arunan HBSCE @Batul Pipewala Kolkata @Saida Sayyed @Aditiya Joshi CUBE what do other say?
Please list those features @Ankit Yadav Xms Work Shop Cube Patkar College, so that others too will get a clear idea on what are the features of Nematodes.

Colonies on potato slices after 48hrs of spreading fermented coconut water from open bottle. Marked blue are colonies similar to that of the ones we have observed on potato slice by @Arunan HBSCE . Marked red are the cottony growth patches. Marked yellow are shiny slimy growth patches. Other white circular colonies can also be seen… 20/8/2021. Kala Talav, Kalyan west. @KIRAN CUBE @Arunan HBSCE @Aswathy Suresh CUBE @Lakshmy PJ CUBE @Batul Pipewala Kolkata @Saida Sayyed
Great!
Which colony is this?
Is it colony of yeast or fungus or any others ?
How can identify?
@Aditiya Joshi CUBE
Congrats for this summary of yesterday’s Chatshaala discussion @Shraddha Sonavane CUBE

Please write a short summary of Cardamine and Psyche butterfly interaction study that you started at CUBE Lab HBBCSE, while working on Cardamine Model system in January 2020 @Shraddha Sonavane CUBE
Control set up please, to compare @Aditiya Joshi CUBE
Hope, you had set up Control. @Aditiya Joshi CUBE

Colonies appear after 48 hours of spreading fermented coconut water from closed bottle… Various colonies can be seen… Marked blue are similar colonies we have seen got by @Arunan HBSCE . We can also see multiple small circular cream color colonies. Some white colonies. And some mucoid or slimy colonies. 20/8/2021. Kala talav, Kalyan west. @KIRAN CUBE @Arunan HBSCE @Seethalakshmy CUBE @Aswathy Suresh CUBE @Lakshmy PJ CUBE @Batul Pipewala Kolkata @Saida Sayyed

Control slices got colonies too!!! They were not inoculated with the fermented coconut water. But growth can be seen… 20/8/2021. Kala Talav, Kalyan west.
[11:36 AM, 8/20/2021] Aditiya Joshi CUBE: The setup has turned smelly… The smell similar to spoilt bread/pav.
[11:37 AM, 8/20/2021] Aditiya Joshi CUBE: There were some fruitflies on the box. A small fly must have manages to go inside… As I opened the box, it flew away…

Observation of slices after 24 hours of inoculation with fermented coconut water from open bottle. We can see some growth or colonies seen. 19/8/2021. Kala Talav, Kalyan west.

Observation after 24 hours from inoculation with fermented coconut water from closed bottle. Some colonies or growth can be seen… 19/8/2021. Kala Talav, Kalyan west.

Control slices after 24 hrs. 19/8/2021. Kala Talav, Kalyan west.
[12:58 PM, 8/20/2021] Aditiya Joshi CUBE: No ma’am. It was not aur tight. A fly managed to enter it…
[12:59 PM, 8/20/2021] Aditiya Joshi CUBE: I kept control slices on open too… But within an hour, they were gone… I think some crows or rats might have taken away or eaten up
[1:27 PM, 8/20/2021] Aditiya Joshi CUBE: Possible goof ups n sources of errors…

1. The box and slices were kept open all the time while spreading the fermented coconut water. Spreading wad done very near or next to bottles kept.
2. No test slices were kept on open.
3. Control slices kept open were lost.
4. Flies were sitting at mouth and inside of open bottle. Also they were sitting on box, lid and one also managed to enter inside.
   5.Though I have washed hands, knife, lid n box, there can be some microbes from these n air n by flies that might have settled on control slices.
5. I also opened it after 24hrs for clicking photos.
   No sir, currently I don’t have any data!
   I think we can start catching mosquito in our locality!
   In collaboration with @Hina Mudgal Kanpur CUBE Ma’am @Seethalakshmy CUBE and others!
   Hi All looking for collaboration on nematode study in the home lab, can you set up some raw potato slices with 4dtops of milk and soil to isolate nematodes and let them multiply on the potato.please reply you can ask questions if any
   Okay ma’am I’d love to collaborate
   what is your plan
   [1:41 PM, 8/20/2021] Ishita Assam: Plan of work

• collect soil sample
• potato slices to serve as medium
• control and sample
• 4 drop of milk at the center to attract the nematodes
  [1:42 PM, 8/20/2021] Ishita Assam: Ma’am when can I see the nematodes in the potato slice
  What about water to keep set up moist?
  From where will you collect soil sample?
  Yes ma’am the soil sample will be damp and also I’d have to keep the potato slice moistened using a tissue paper at the bottom
  After 24 to 48hrs of set up you should be able to see a small microbial pool on the potato slice and at the edges of this some worm like organisms 1 to 2mm in size moving
  [1:44 PM, 8/20/2021] Ishita Assam: I’d have to collect from an area where there’s a lot of trees and where leaves fall and rot
  [1:44 PM, 8/20/2021] Ishita Assam: Should I also cover it in case sone other insect lays in it?
  Since C elegans has not been found in rotting leaves but has been found in rotting fruits or vegetables soil from such a place is advisable
  Rotting fruits and vegetables, where can I get such soil then ma’am
  [1:46 PM, 8/20/2021] Batul Pipewala Kolkata: Yes but not airtight otherwise a lot of fungus grows.Insect larvae are inevitable but nothing to worry they do not harm the nematodes as I have seen in my set ups
  [1:48 PM, 8/20/2021] Batul Pipewala Kolkata: From under any Fruiting tree like jamun for example
  Okay ma’am
  Other nematodes will definitely be present in soil from other places@@Ishita Assam
  [1:49 PM, 8/20/2021] Ishita Assam: I’ll update when I start this
  [1:50 PM, 8/20/2021] Ishita Assam: Okay ma’am I will try and collect such but if I don’t I would continue with normal soil from garden and all
  Why not we do a search for to see any colony morphology resemblance? @Aditiya Joshi CUBE
  I’ll try too
  Why not? “Ideally”, won’t we expect a sample of microbes from the atmosphere or knife used for slicing potato etc.? @Aditiya Joshi CUBE
  what is your plan?
  Can’t it be the young ones! Any reference? @Batul Pipewala Kolkata
  Was the photo taken intentionally not to have light on the full slices? @Aditiya Joshi CUBE
  Yes sir. From knife, air, my hands
  Control seems to show clear colonies. Any interpretation?
  No sir. The shadow is of walls of box. N in flash light colonies were not seen much. It was shining. But yes, its opportunity for me to learn how to take better pictures n focus.
  [2:01 PM, 8/20/2021] Arunan HBSCE: Let’s start! Don’t you think this rainy season may have resulted in a surge in mosquito breeding places and mosquitoes? @Abhijeet Singh
  [2:03 PM, 8/20/2021] Arunan HBSCE: What is your guess? @Ishita Assam instead of we waiting for instructions! @Ishita Assam
  [2:05 PM, 8/20/2021] Arunan HBSCE: Can’t we challenge this? @Ishita Assam
  [2:05 PM, 8/20/2021] Arunan HBSCE: What if you do not cover it? @Ishita Assam
  [2:07 PM, 8/20/2021] Arunan HBSCE: How about designing an experiment to challenge this? @Ishita Assam
  [2:09 PM, 8/20/2021] Arunan HBSCE: Only trees? Why not under fruiting Phyllanthus plants and several others? @Ishita Assam @Batul Pipewala Kolkata
  Any clear references?
  I can collect soil samples from non fruiting area and an area where fruits fall and rot but then the thing is I won’t be sure if I’ll get C.elegans or not
  Why not post the full Design now itself, after stating the objective?@Ishita Assam
  Sir last time I got larvae in my potato slice
  Could not follow y you wont be sure if it is C elegans?Is it because you need a microscope to see their features@@Ishita Assam ?
  [2:13 PM, 8/20/2021] Arunan HBSCE: Why not do both, with a clear design starting from now? @Ishita Assam @Batul Pipewala Kolkata
  [2:13 PM, 8/20/2021] Arunan HBSCE: Design, please @Aditiya Joshi CUBE
  Yes sir I can design an experiment
  [2:16 PM, 8/20/2021] Arunan HBSCE: Please post those evidences @Ishita Assam Please give a summary and state where you got stuck?
  How are you going troubleshoot, this time? @Ishita Assam
  [2:17 PM, 8/20/2021] Arunan HBSCE: Let’s start! @Ishita Assam
  Yes sir
  What could be your research question in soil Nematodes? @Ishita Assam
  On 20th August 1897, Sir Ronald Ross discovered that Anopheles mosquitoes were responsible for the transmission of the malarial parasite. To mark this breakthrough discovery in the fight against malaria, each year we celebrate 20th August as
  World Mosquito Day
  @ hina allen cube kanpur.
  
  

MOSQUITO prevention and control. Check out for MOSQUITO breeding sites to eradicate this vector from spreading diseases like , DENGUE, malaria, chikungunya and more . @ hina, rahil , samiksha, utkarsh, vyom .

VIDEO SHARED BY BATUL AT 4.32PM
BREAKING NEWS The pharynx structure of my soil nematode looks v much like Caenorhabditis elegans from the reference given below.looking for your comments @⁨Dr Arunan⁩ @⁨Anshu Kadam⁩ @⁨Aditya Joshi⁩ @⁨Ishita⁩ @⁨Shahin🍁⁩ @⁨Akanksha⁩ video from Homelab Kolkata BatulP 20/8/21

Reference
https://www.wormatlas.org/albertson_1976/Albertson_1976.html
@Arunan HBSCE @Anshu Kadam (Nematodes) @Aditiya Joshi CUBE @ @Shahin​:maple_leaf:

VIDEO SHARED BY BATUL AT 4.45PM
As given in the reference a is the buccal cavity, b is the pro corpus, c is the meta corpus and d is the terminal bulb.video taken under 45X of compound microscope. Cube Homelab Kolkata BatulP 20/8/21
MOSQUITO prevention and control. @ cube kanpur
Presentation on cardamine made by @Priti Kanade in 2017 for National Meet!
@Arunan HBSCE @KIRAN CUBE @Saida Sayyed @Shraddha Sonavane CUBE @Seethalakshmy CUBE @Lydia cUBe Drosphila @Aswathy Suresh CUBE @Batul Pipewala Kolkata

S4E150 CUBE chatShaala: Cardamine: context to understand Gene expression and KNOXX gene

CUBE ROOM LINK Please click on the CUBE (BLUE) to join the CUBE ChatShaala Timing:5.30 pm to 9.30 pm Kindly add your place/affiliation along with your name while joining. Please click on the below given link if you face any issue in joining the webinar, i hope this would help in resolving any issue in joining. Thanks Looking forward to your participation

Kindly click on the above👆🏻 given link for the summary of last discussion.

Invitation to join today’s CUBE chatShaala,
Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in all CUBE what’s app groups and on metastudio after the session

Here’s the direct link of the CUBE webinar: https://webinar.hbcse.tifr.res.in/b/kir-hye-cn4
Note: we have changed the link to avoid technical error

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.
SLTT-J
Single line culture of fruit flies.
Started on 16/08/2021,11:40 AM. Date of video: 19/08/2021
TRSV medium - 15/08/2021, 11:00 PM

Expectation:
Pupae on fourth or fifth day.
New flies on 8 th day.

Observation on 19/08/2021, 3:57 PM
~15 larvae
~25 eggs
20/08/2021, 12:18 PM ~15 larvae , ~25 eggs
Location : Thanniam, Thrissur, Kerala
Sidhy.P.P, CUBE S N College , Nattika.
SLTT-H
Single line culture of fruit flies.
Started on 16/08/2021, 11:40 AM , Date of video: 19/08/2021
TRSV medium - 15/08/2021, 11:00 PM

Expectation:
Pupae on fourth or fifth day.
New flies will emerge on 8 th day.

Observation on 19/08/2021, 3:56 PM
~ 30 larvae
~ 20 eggs
20/08/2021,12:20 PM : ~ 40 larvae , ~20 eggs
Location : Thanniam, Thrissur, Kerala
Sidhy.P.P, CUBE S N College , Nattika.

VIDEOS SHARED BY SIDHY AT 5.16PM
SLTT-I
Single line culture of fruit flies.
Started on 16/08/2021, 11:40 AM. Date of video:19/08/2021
TRSV medium - 15/08/2021, 11:00 PM

Expectation:
Pupae on fourth or fifth day.
New flies on 8 th day.

Observation on 19/08/2021, 3:56 PM
~ 15 larvae
~ 20 eggs
20/08/2021, 12:18 PM ~ ~25 larvae , ~20 eggs
Location : Thanniam, Thrissur, Kerala
Sidhy.P.P, CUBE S N College , Nattika.
MOSQUITO prevalence in water accumulation, Rainy season are hot spots for them to breed . Check out for them. Spread awareness of this vector. Kill it before it spreads another epidemic . @ hina cube kanpur , dated 20. O8.21
A fruitfly on onion piece… 8:19 pm. Kala Talav, Kalyan west. 20/8/2021
Pic by salman mustafa, location swaroop nagar. Time 7.00pm
20.08.21

• Needs better focus .
  @ hina Allen .

What is RER test? Is it possible to make alternatives to perform RER at home?

How can we culture Earthworms easily using simple things at home?

RER Test Experiment : Making a design of experiment how to perform in a simple way that every ones can do it in their home labs!

Which kind of nutrition will get earthworms culturing by newspaper?

What are the components presents in Newspaper?

Cellulose are carbohydrates!

What is the difference between starch & cellulose?

Beta Glucose & Alpha Glucose Differences?

@Arunan HBSCE sir @Aditiya Joshi CUBE @Abhijeet Singh @Seethalakshmy CUBEObjective was to observed after how many Compound leaves first flower buds arose.
519 th day of CUBE ChatShaala: 20th August 2021.

https://www.google.com/maps/d/edit?mid=130gpg9gUtd61uh2e-7vDbctkkfTpu4xq&usp=sharing

18 CUBists from 18 Centres had joined the webinar.

INDIA

Assam
Ishita Sonowal: Jorhat

Delhi
Aastha Ahuja: ANDC

Haryana
Aashi: Panipat

Kerala
Aswathy Suresh: Thriprayar
Seethalakshmi CR: Pazhuvil, Thrissur
Siddhy: Thanniam Thrissur

Maharashtra
Abhijeet Singh: Ulwe, Navi Mumbai
Aditya Joshi: Kalyan
Arunan MC: Breach Candy, South Mumbai

Uttar Pradesh
Kiran Yadav: Moradabad
Divyanshi Chauhan: Noida
Ravleen Kaur: Bijnore

Uttrakhand
Sakshi

West Bengal
Batul Pipewala: Kolkata

Meena
Medha
Prachi Pant
Deeksha Pandey

Note: Cubist(s) who had joined today but their name isn’t included in the list, kindly replied to this message[12:26 AM, 8/21/2021] Shraddha Sonavane CUBE: Cardamine- Psyche butterflies interaction

Cardamine is new host plant for Psyche butterflies.

While working on Cardamine model organism in January 2020, initially I was observed one Caterpillar on Cardamine plant​:seedling: in 2nd right side patch of Cardamine forest in front of HBCSE main building.
I was curious about caterpillar so I was collected caterpillar with same Cardamine plant on which caterpillar found.
I kept same plant in container having some water so all roots dipped inside Water.
Then,This container & some more Cardamine plants were added in another container. both containers were kept in glass tank.
One Open side of glass tank was tightly covered with net & rubber band.

After 4-5 days I observed Caterpillar was turned into green pupa.In between 4-5 days, I observed that Caterpillar was eaten most of leaves & fecal matter was there.

After 5-6 days, new adult white butterfly with prominent black spot on upper wing was emerged.

Because of their appearance I could easily found that their name - Oriental Psyche (Leptosia nina nina)
[12:27 AM, 8/21/2021] Shraddha Sonavane CUBE: Because of more observations I could found one Egg & 6 more Caterpillars on Cardamine plants

The Egg was cylindrical shape & white in colour on terminal leaflet in upright standing manner.

Again Similar set up were made to study life cycle of newly found Caterpillars.

In these set up I could observed 2 different pupa colour- 1 green colour &
2. Yellowish brown/Creamish colour.

But when adult butterflies emerged, I could observe
2 different eyes👀 colour- 1.Brown colour
2.green colour
Summary of 519th day of Cube Chatshaala*
We started the discussion with what I(Abhijeet) have plan for the Chai and Why? Programme.
So I’ve worked on Model Organisms like Mosquito, Earthworm, and Snails when I was active in cube in my secondary school (7th to 9th std)
So today we discussed about Earthworm.

How can we culture and maintain Earthworms in our Homelab?
So culturing earthworms at home would be very easy! We don’t need high-tech laboratory stuff. We just need a plastic cup, paper, earthworm and waste cloth

1. Take cup and pinch holes at bottom with the help of a pin.
2. Make pieces of paper, and fill 1/3rd of the cup.
3. Make that paper inside cup moist with water
4. Put earthworm inside cup.
5. Cover the cup with cloth and rubber band.
6. keep the cup(s) inside a tray with newspaper wetted with enough water .What’s special in Earthworms?
   Humans have Spinal cord which helps in making co-ordination between different body parts in each activity that we do throughout. If due to some reason the spinal cord is injured we get paralyzed and the co-ordination is lost.

Earthworms have Ventral Nerve Cord(VNC) with same function as Spinal cord in humans i.e maintaining coordination in body. But if VNC of earthworms get injured it gets regenerated

So can we study how this regeneration happens in earthworms but not in humans!!!

RAPID ESCAPE RESPONSE TEST (RER) was designed to see how in how much time this regeneration of VNC occurs and also for selecting earthworms with good co-ordination in their body.

How is RER test performed!?

1. Make salt solution of a particular concentration.
2. put some 2-5 ml of that in test-tube(experiment)
   3)take another test-tube fill with 2-5ml of water(control)
3. add one earthworm at a time in both and watch their response within the 2 min time
4. note the observation.
5. Remove the earthworm from soln and wash is very nicely and keep the back in culture
   Expectations:-
6. The earthworm should immediately response towards the salt solution and escape from it.

Inference:-

1. As the salt solution is harmful for earthworms they should escape from it showing excellent co-ordination. And earthworm in water(control) would not show any response towards water.
2. Earthworm which showed the coordination and came out of salt solution can be selected for studying.
   After RER test we can use the earthworm that passed the test for studying the time it takes for them to regenerate their VNC.

On the ventral part we give a lesion(enough that we can say that VNC is lesioned).
After giving the lesion perform the RER test to confirm that earthworm’s body co-ordination is lost.
Expectations:- The earthworm should not be able to escape the salt solution.

Continue doing RER test with 2 days interval till the VNC is regenerated and the co-ordination is gained again.

@Aswathy Suresh CUBE can you please comment on how can we perform RER in our homelabs!
@Aditiya Joshi CUBE @Seethalakshmy CUBE please comment on the nutrition that can be derived by earthworm by eating paper in cup cultures!!!When I (Shraddha) & @KIRAN CUBE mam did search on Leptosia nina - Wikipedia & https://www.ifoundbutterflies.org/sp/811/Leptosia-nina/early-stages
for host plants of Psyche butterflies, we found that Cleome,Capparis are host plants but not Cardamine plants