Questions addressed in today’s Chatshaala:
1)What is budding in Chlorohydra?
2)How does budding differ in Hydra and plants?
3)What is the difference between budding and grafting?
4)What are the uses of grafting in plants?
Created context:
If an Alphonso (scion) is grafted onto a wild mango (stock), what is the expected outcome for the mango formed from them?
Alphonso on an Alphonso branch
Wild mango on a wild branch
For example, if we bud a pink-colored hibiscus onto a red-colored hibiscus, what flowers can be expected?
Can there be a mixture of red and pink petals?
Seethalakshmi expects a mixture of red and pink hibiscus flowers through grafting. Is that possible?
5)How can genetic variation occur,pollination?
We discussed Mendel’s theory, including the dominance and recessive nature of alleles, as well as homozygous and heterozygous crosses.
Created context:
If the grafted Alphonso tree has a 15-year lifespan and the wild mango tree has a 30-year lifespan, what will happen to the Alphonso after 15 years?
Enas says there’s an 80% chance that the wild tree will support Alphonso’s growth.
I said there’s a 50% chance, since aging could occur in the cells after 15 years, as the lifespan is mentioned as 15 years.
6)How do telomeres and telomerase play a role in aging?
Telomeres, the specific DNA–protein structures found at both ends of each chromosome, protect genome from nucleolytic degradation, unnecessary recombination, repair, and interchromosomal fusion. Telomeres therefore play a vital role in preserving the information in our genome. As a normal cellular process, a small portion of telomeric DNA is lost with each cell division. When telomere length reaches a critical limit, the cell undergoes senescence and/or apoptosis.
The level of telomerase activity is important in determining telomere length in aging cells and tissues.
Telomerase and the aging process - PMC.
TINKE moment of the Day:
Recent studies have documented that grafting enables exchanges of both RNA and DNA molecules between the grafting partners, thus providing a molecular basis for grafting-induced genetic variation.
Our results demonstrate that inter-species grafting in plants could produce extensive and heritable alterations in DNA methylation. We suggest that these readily altered, yet heritable, epigenetic modifications due to interspecies hetero-grafting may shed one facet of insight into the molecular underpinnings for the still contentious concept of graft hybrid. Inter-Species Grafting Caused Extensive and Heritable Alterations of DNA Methylation in Solanaceae Plants - PMC.
Grafting and budding are horticultural techniques used to join parts from two or more plants so that they appear to grow as a single plant. In grafting, the upper part (scion) of one plant grows on the root system (rootstock) of another plant. In the budding process, a bud is taken from one plant and grown on another.
Grafting and Budding Nursery Crop Plants | NC State Extension Publications.
Layering is a vegetative propagation technique where the stem or branch of a plant is manipulated to promote root development while still attached to the parent plant. Once roots are established, the new plant can be detached from the parent and planted. Layering is utilized by horticulturists to propagate desirable plants.
In hydra, a bud develops as an outgrowth due to repeated cell division at one specific site. These buds develop into tiny individuals and, when fully mature, detach from the parent body and become new independent individuals.

