Circadian Rhythm study in fruit fly (Drosophila)

A Simple experimental set up has been designed by the CUBists to study Circadian Rhythm or sleep wake /activity pattern in fruit flies in CUBE HOME LABs

Cubists have done this work in collaboration, Cubists had discussed in Cube Chatshaala that what minimum things are necessary to study the Circadian Rhythm in fruit flies and to attract them.
1. A BAIT
2. PLACE
3. OBSERVATION

Tomato slices as bait to attract fruit flies , slices were placed on a sheet of paper near the trash bin and it was observed (number of fruit flies visiting the bait)after one hour for five days.
Below are the observation for following days:
started: 03/08/2020,10 pm
Location Bottukadavu Kerala
latitude and longitude: 10 degree N to 76 degree E
Temperature: 24 degree C
Sunrise Time: 06:15 am
Sunset Time: 06:48pm
Number of flies on tomato 01
IMG-20200804-WA0000
Following are observations of this experiment
image

Based on the reading a graph has been plotted

image
This is a five days graph, X-axis- Time, Y-axis Number of fruit flies. This was done by @saswathy679
@saswathy679 @sidhypp @saida786110
What does this graph depicts.

image
Based on this observations we discussed why such pattern is seen , How does this pattern being regulated?
image
Very basic model was constructed in order to understand the regulatory machinery

Further more to take this work forward to get reproducible results @Seema Suthar from Parvorim Goa and Naik Riddhi, they both have taken the initiative and started observing the activity pattern of fruit flies in their home labs.
image
Date - 6 Sept 2021
Observation time : @9am
Tempt - 26°C
Latitude: 15.4942° N,
Longitude: 73.8215° E
Place - St.Inez , Panaji- Goa
No. of fruitflies observed - around 50 (including the one’s near the place) Data Collector - Seema Suthar (MHSS CUBE GOA)
image
Some ( around 10 of them ) of the Fruitflies are not on banana… their stomach became full? :sweat_smile: Or they didn’t like taste of banana​:face_with_monocle:…? Or amonst those who like to live away from grps jst like humans? Or jst simply they r sitting der for sometime? :thinking:
Photos of experiment by Seema Suthar

photo_2021-09-08_13-59-10
Naik Riddhi

image

6 Likes

Very nice. What is the explanation of the pattern seen?

3 Likes

All India Fruit fly trap CUBE Mela (4.30pm to 5.30pm) a collaborative experiment was conducted by the CUBist where bottle traps were kept by them at three different locations in order to verify the hypothesis ( Fruit flies are more active in evening from 4.30pm to 8.00pm and this could be the crucial time to trap fruit flies easily) this conclusion has been made after observing the graph of previous studies on activity pattern in fruit flies.

This experiment was conducted by 15 CUBists from across the India.

GOA
Porvorim- Naik Riddhi
Porvorim- Yuga Prabhudesai
Porvorim- Sarvada Sail
Porvorim- Ankit Yadav
Panjim-Seema

JHARKHAND
Kokar (Ranchi)- Man Masih Beck @manmasih
Moradabdi (Ranchi)- Rechel Tirkey @Rechel_tirkey

KERALA
Bottukadavu Thrissur- Aswathy Suresh
Kandassankadavu - Lakshmy PJ
Pazhuvil, Thrissur- Seethalakshmy
Thanniam Thrissur- Sidhy

MAHARASHTRA
South Mumbai- MC Arunan
Prabhadevi Mumbai- Yash Sheregare
Panvel Mumbai- Shraddha Sonawane
Isha Pawale- Kolhapur
Wardha- Janhvi Deshmukh

WEST BENGAL
Kolkata- Batul Pipewala

Following are the glimpses of this experiment

image
Kokar (Ranchi)- Man Masih Beck @manmasih

image
Aswathy Suresh from Bottukadavu Kerala @saswathy679

image
Janhvi Deshmukh from Wardha @Janhvi_deshmukh17

image
Riddhi from Goa @Riddhi

image
Lakshmy PJ from Mampilly Kerala @Lakshmy

image
Rechel Tirkey from Morabadi Ranchi @Rechel_tirkey

image
Isha Pawale From Kolhapur @Isha_23

image
Yash Sharegare from Prabha devi Mumbai @yash_sheregare

image
Yuga Prabhudesai from Goa @Yugh23

image
Sarvada From Goa @sarvada

image
Arunan
image
MC Arunan from South Mumbai @Arunan

image
Batul Pipewala from Kolkata @magpie

image
Seethalakshmy from Kerala @2020ugchsncnseethala

image
Sidhy from Thanniam Thrissur, Kerala @sidhypp

3 Likes