Here we are trying to design a protocol that would exactly be like the standard corn mean media that we make in the lab for our standard CsBz flies i.e. Drosophila melanogaster flies.
It would be better if I explain the method of preparation of the standard corn meal media (250ml).
Dextrose - 12.5gms
Sucrose - 6.25gms
Maize powder - 20.75gms
Yeast extract - 3.75gms
Agar - 4gm
Mix all these components in water.
Add water to make up the volume of the mixture to 250ml
Mix all the components in the water well.
Then cook the media mixture on a medium flame.
Cook till all the components in the media are homogenised completely and that there are no lumps in the media.
Next comes autoclaving
We autoclave using a pressure cooker.
The reason for autoclaving is to kill the spores and microorganisms present and to sterilize everything.
We sterilize the glass bottles and the media by autoclaving in a pressure cooker.
Trying maximum to minimize the possibilities of getting any fungal or bacterial growth we autoclave
Once the media is autoclaved,
We add acids in the 250ml media
Orthophosphoric acid - 0.17ml
Propionic acid - 1ml
This is what we want to do exactly in our homemade media.
So that we can standardize our homemade media as well.
This is a site as a reference for the standard cornmeal media that we make…