Summary of 15th June 2020 CUBE Chatshaala Webinar:
We started with @Arunima CUBE Kozhikode from SN College Kozhikode, Kerala.
She told about her cardamine cultureâŚ
We want @Arunima CUBE Kozhikode to post the summary of her work and her further plans.
We moved on to @Aswathy CUBE Thriprayar, Nattika from SN College Nattika, Kerala.
She gave a summary of her single line cultures that she is maintainingâŚand now posting pictures of her identification work.
@Aswathy CUBE Thriprayar, Nattika concluded that the flies belonging to the single lines do not belong to the family Drosophilidae.
But most of us werenât convinced as there wasnt any picture clearly showing the veins of the wingâŚ
@Aswathy CUBE Thriprayar, Nattika said that she can see just 1 break in the costal vein instead of 2 breaks in the costal vein of the wing of a Drosophilidae fly.
We need some reference pictures⌠whenever we post the picture of the wing of a fly we should post it along with the CsBz fly wing which will help us in giving evidences as well as convincing everyoneâŚ
Why so???
Because CsBz fly belongs to the Drosophilidae familyâŚso we can have a comparison there.
Iâll be posting some reference pictures belowâŚafter the summary.
@Hina Maâam CUBE Kanpur maâam too added along with @Aswathy CUBE Thriprayar, Nattika. She too will be joining @Aswathy CUBE Thriprayar, Nattika in the identification work by simultaneously starting to identify the flies in her mixed culture bottles.
@Yash CUBE and others contributed to this discussion.
We had an old CUBist from Delhi⌠@Kanishka from Jamia Millia Islamia, New DelhiâŚshe was earlier in Dyal Singh college, Delhi.
She contributed a lot with her experience on working with cardamine and the seed coat germinationâŚshe helped out @Nazish CUBE in the discussionâŚhad a great discussion with her.
@Saida Elph CUBE then told about her work that she is doingâŚcircadian rhythm of fruit flies.
The genes involved in the circadian rhythm of fruit flies and then related it with Phyllanthus plant.
There would be genes of the circadian rhythm or the biological in the Phyllanthus plant tooâŚ
@Lakshmy CUBE Kerala, @Mandar CUBE and others can look for itâŚ
@Mandar CUBE from Ratnagiri gave updates about the Phyllanthus plant that he has in his home lab. Expecting him to proceed with its identification.
@Saida Elph CUBE also had a problem in differentiating between a larva and a pupa of a fruit fly as she is new to handling and culturing fruit flies.
@drishtantmkawale & @Yash CUBE helped her out by showing some basic differences in a larva and a pupa of a fruit flyâŚ
A larva eats and is very active and moves around in the bait or the mediaâŚ
But a pupa isnât motile âŚit stays at one place itselfâŚ
This was a short summary.
Summary by @Kanishka (Kanishka Parishar from Delhi)
Thers was discussion over⌠Fruit fly single line culture and in related the media preparation. Exciting was knowing media preparation by people at home using rava, tomato, sugarâŚ
Nd how to avoid contamination they thought to used acid (vinegar) so as to drop the pH.
Also there was discussion on seed germination rate⌠Of cardamine seeds⌠The method to organise setup. Discussion over factors which affect seed germination.
Next we discussed about kanamycin antibiotics how it will affect the seed growth. By floral dip method, the students are planning to introduce the gene and then study its role.