Fluorescence, Luminescence:- Let's light up the discussion!

Fluorescence, Luminescence:- Let's light up the discussion!
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Happy New Year Everyone!!

The year 2020 at CUBE HBCSE, Mumbai started with a bang as it marked the end of the CUBE X’Mas Workshop 2019.

Yesterday, we had a crucial discussion on the basic difference between the often misunderstood as one terms florescence and bioluminescence.

These were discussed on the context of the fluorescent bacteria being cultured by the Rotifer group @khushdeep @saida786110 of CUBE Elphinstone as a part of their long term objective of HGT (Horizontal Gene Transfer) in Rotifers

But before we jump on those big words like Horizontal Gene Transfer, we need to understand basic terminology which often leads to misconceptions during the crucial discussions.

So yesterday, with the help of Google Baba and inputs from everyone (CUBists from Patkar College, Elphinstone College, Ramniranjan Jhunjhunwala College, @jaikishan Sir and @Arunan Sir), FLORESCENCE was discussed as the phenomena occuring when an atoms’ electron get excited to the higher energy level (higher orbital) by an external energy source, so while getting excited, the molecule acquires energy. Some part of the same energy is retained and is later emitted when the lower energy level (lower orbital) is achieved which we see in the form of illumination or florescence

BIO-LUMINESCENCE was defined from the inputs of fellow CUBists and ofcourse with the help of Google Baba as a phenomena or chemical reaction occuring when an enzyme is broken down and in this process, light is evolved as the by-product.

Here, the enzyme Luciferase gives illumination.
The bacterium isolated from the Squid is Aliivibrio fischeri.

I would leave the rest to you guys who are reading this and ofcourse the group working on this!
And I would contribute too!!

Fig5

An image of a chemical reaction of bioluminescent enzyme luciferase which involves the evolution of CO2 and light is given out in fireflies.
Source: Google Images

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Are you sure light is emitted in Bioluminescence when Luciferase enzyme breaks down…? :hushed:

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I am not completely sure about this!
But yesterday, there was some discussion on the enzyme-substrate thing of which I missed some part.:grimacing:

How about doing a quick search on Luciferase…? @drishtantmkawale

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Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein. The name was first used by Raphaël Dubois who invented the words luciferin and luciferase , for the substrate and enzyme, respectively.

Reference: Luciferase - Wikipedia

The Bioluminescence reaction of LuciferIn is an enzyme bound reaction, enzyme being luciferase,the complex is bound to the enzyme through out the light emitting stages, namely
1 formation of luciferyl adenylate complex ,
2 reaction with oxygen and other intermediate steps till the excited stage
3 release of light energy
4 formation of ground state of the product
Are all enzyme bound reactions

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Please explain why this reaction causes energy to be released in the form of light, as against other, more commonly seen (or more correctly, felt), exothermic and endothermic reactions.


Luminance bacteria it is glow only in dark place

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Or do they appear to us only when they are in the dark places?

Yes sir luminance is glow only dark place because of may be in luminicence condition they not absorb light they emit light do few chemical reaction .

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When you say " all are enzyme bound reactions" , what does this mean? @magpie

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What did you do with colony?
What are further plans? @khushdeep

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We have the culture of luminescent bacteria …
We have to feed the bacteria to the rotifers how will we feed the rotifers
We dont have any idea about that … any one can help us
For the same .???

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Oooo
This is a serious thing!

You have cultured bacteria but don’t have any idea to feed them!
Ok cool

How do you feed the Rotifers normally while maintaining a culture of theirs?

Why do you need a separate method to feed the Rotifers?


in this article are shown hiw luminance bacteria are glow and which gene are responsible for luminance…

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luciferases enzyme will convert the luciferin substrate in to the oxyluciferin prouduct.

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Do you mean to say, in majority of cases the energy is released in he form of heat, right? But in this case the excess energy is released in the form of light when the excited molecules come back to ground level, right? @vvcstemplay @drishtantmkawale

I have another query: what is the difference between fluorescence and luminescence? @drishtantmkawale @vvcstemplay @Jade23 @Swati @G_N and others…

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“For several decades, bacterial bioluminescence has been viewed as the quintessential example of microbial group behavior. Light production from a single, isolated bacterium appears to be both biologically irrelevant and energetically wasteful. However, the overall emission from a group of cells is sufficiently high…” Doesn’t it resemble COLLABORATION in CUBE? Alone, understanding an article will be inefficient and difficult…! Together, it will be illuminating! @khushdeep @drishtantmkawale @vvcstemplay @G_N…and others!

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Where is the “light” in the process?!! @Swati

Fluorescence is the re emission of light at a different wavelength after absorbtion.
Luminescence is the production of light.
Phosphorescence is the emission of visible light after being energized even after the energizing source is removed.

White LEDs have a phosphor that converts UV and blue light to white light. The white LED is actually a UV-Blue LED.
Fluorescent lamps convert electron stream into UV light via mercury vapour, which strikes a phosphor to produce light.

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