Fruitfly:Activity pattern Vs Phyllanthus:Leaf folding movements

Theertha · June 10, 2024, 3:53pm

A) FRUITFLY ACTIVITY PATTERN:

Objective: To observe the activity pattern of fruit flies using a tomato slice and compare it with _Phyllanthus urinaria_leaf folding movements.

Hypothesis: A greater number of fruit flies can be observed during the day compared to at night.

Any guesses on how many fruit flies will be attracted to this tomato slice after 2 hours? @⁨Batul Ma’am⁩ @⁨Smiti⁩ @⁨Aastha⁩ @⁨~Enas⁩ @⁨~Khushi ⁩ @⁨Amirtha CUBE⁩ & othes
Setup Kept near to kitchen.
Place : Cherukkad, Kozhikode
Date:8/06/2024
Time:9.13am
Theertha.

Congratulations for the prediction @⁨Batul Ma’am⁩ @⁨Aastha⁩ &@⁨Arunan sir ⁩ .Here is the result after 2 hr.Approximately 75 Fruitflies observed after 2 hrs.
Time of setup :9.13am,8/06/2024.
CUBE Homelab cherukkad Kozhikode.
Date:8/06/2024,Time:11am, Theertha. Any guesses on the Fruitflies at 1pm ? @⁨Batul Ma’am⁩ @⁨Aastha⁩ @⁨Arunan sir ⁩ & others.

Approximately 110 Fruitflies where observed at 1pm.
CUBE Homelab, cherukkad Kozhikode.
Date:8/06/2024,Time:1pm Photo :1pm.Photo : Theertha.
@⁨Aastha⁩ The picture that I have shared on 11am, i got flies count as 74 &76 .Could you also count these two pictures and comment on the number of flies?

Approximately 130 Fruitflies where observed at 3pm.@⁨Aastha⁩ please comment your counting .
CUBE Homelab, cherukkad Kozhikode.
Date:8/06/2024,Time:1pm Photo :3pm.Photo : Theertha.

Approximately 150 Fruitflies observed at 5pm , CUBE Homelab cherukkad Kozhikode,Date:8/06/2024.Theertha.

Breaking news:
A large decrease in the number of Fruitflies from from 150 to 11,5 pm to 7 pm.@⁨Batul Ma’am⁩ @⁨Himanshu Joshi⁩ & others.
Number of Fruitflies:11 @⁨Aastha⁩ now is it easy to count ?
CUBE Homelab, cherukkad Kozhikode.
Date:8/06/2024.
Time :7pm.
Theertha.

Number of Fruitflies observed:21 at 9.12 pm .CUBE Homelab cherukkad Kozhikode.Date:8/06/2024.Theertha.

12 Fruitflies were observed at 11pm on 8/06/2024.CUBE Homelab cherukkad Kozhikode.Theertha .

SETUP:2

Objective: To observe the activity pattern of fruit flies using a tomato slice and compare it with Phyllanthus leaf folding movements.

Hypothesis: A greater number of fruit flies can be observed during the day compared to at night.
@⁨Himanshu Joshi⁩ .i have restarted where I left on 8/06/2024.
Placed a slice of tomato on the white paper and kept it the kitchen.
Time :11pm
Date:9/06/2024.
Place: cherukkad, Kozhikode
Theertha.

One fruitfly observed at 1am.
Time :1am.
Date:10/06/2024.
Place: cherukkad, Kozhikode
Theertha.

Zero fruitsflies at 3am .
Time :3am
Date:10/06/2024.
Place: cherukkad, Kozhikode
Theertha.

Again,Zero fruitsflies at 5am .
Time :5am
Date:10/06/2024.
Place: cherukkad, Kozhikode
Theertha.

Observed 3 Fruitflies at 7am…
Time :7am
Date:10/06/2024.
Place: cherukkad, Kozhikode
Theertha.

Observed 7 fruitflies at 9am.
Time :9am
Date:10/06/2024.
Place: cherukkad, Kozhikode
Theertha.

21 Fruitflies observed at 11am.
Time :11am
Date:10/06/2024.
Place: cherukkad, Kozhikode
Theertha.

41 Fruitflies observed at 1pm.
Time :1pm
Date:10/06/2024.
Place: cherukkad, Kozhikode
Theertha.

47 flies at 3.24pm.
Time :3.24pm
Date:10/06/2024.
Place: cherukkad, Kozhikode
Theertha.

~100flies at 5pm
Date:10/06/2024.
Place: cherukkad, Kozhikode
Theertha.

25flies at 6.38pm
Date:10/06/2024.
Place: cherukkad, Kozhikode
Theertha.

14fruitflies observed at 9pm
Date:10/06/2024.
Place: cherukkad, Kozhikode
Theertha.

12fruitflies observed at 11.30pm

Date:10/06/2024.
Place: cherukkad, Kozhikode
Theertha.

No.of fruitflies :0
Time :1am
Date:11/06/2024.
Place: cherukkad, Kozhikode
Theertha.

No.of fruitflies :0
Time :3am
Date:11/06/2024.
Place: cherukkad, Kozhikode
Theertha.

No.of fruitflies :0
Time :5am
Date:11/06/2024.
Place: cherukkad, Kozhikode
Theertha.

No.of fruitflies :37
Time :8.30am
Date:11/06/2024.
Place: cherukkad, Kozhikode
Theertha.

Theertha · June 11, 2024, 5:35am

Number of Fruitflies:26.
Time :9am
Date:11/06/2024.
Place: cherukkad, Kozhikode
Theertha.

43frutflies observed at 11am.
Time :11am
Date:11/06/2024.
Place: cherukkad, Kozhikode
Theertha.

Theertha · June 11, 2024, 12:01pm

~185Frutflies observed at 1pm.
Time :1pm
Date:11/06/2024.
Place: cherukkad, Kozhikode
Theertha.

~122Frutflies observed at 3pm.
Time :3pm
Date:11/06/2024.
Place: cherukkad, Kozhikode
Theertha.

~92Frutflies observed at 5pm.
Time :5pm
Date:11/06/2024.
Place: cherukkad, Kozhikode
Theertha.

29 Frutflies observed at 5pm.
Time :7pm
Date:11/06/2024.
Place: cherukkad, Kozhikode
Theertha.

25Frutflies observed at 5pm.
Time :9pm
Date:11/06/2024.
Place: cherukkad, Kozhikode
Theertha.

19 Frutflies observed at 5pm.
Time :11pm
Date:11/06/2024.
Place: cherukkad, Kozhikode
Theertha.

GN · June 12, 2024, 1:10am

Are you keeping a fresh-cut tomato each day?

Just getting curious: If we keep more than one piece, would the same number of flies distribute over them, or would more flies visit?

KiranyadavR · June 12, 2024, 3:10pm

Inspired by @Theertha, Another slice of tomato was also kept on the same day. @Amritha from Palakkad Kerala also kept a slice of Tomato on a plane sheet of paper.

Date of set up: 08.06.2024 at 11.00 am

Theertha · June 13, 2024, 12:11pm

I have changed the tomoto slice twice .
One tomato slice used from 11 am 11 pm on 8/06/2024 and that changed and added another tomato slice at 11pm ,9/06/2024 and continued observations for 2days in 2 hr interval.

I thought that if I kept 2 tomoto slices, I will get equal number of flies at each of them .

I made a set up to see this .

Kept 2 slices of tomato on a white paper and observed it .
Time of setup:8.41am
Observations:9.47am
Time :
A(left side):12
B(Right side ):10

Time :10.44am
A(left side):31
B(Right side ):12

Time :12.04pm
A(left side):36
B(Right side ):29

Time :2.57pm
A(left side):60
B(Right side ):36

Time :4.52pm
A(left side):30
B(Right side ):80

Till 2.57pm, number of Fruitflies higher in setup A than setup B .But at 4.52 pm A>B.
sorry that I haven’t followed 1hr intervel properly.May this continue? Or it is an exception case observed at 4.52pm ?
@GN @KiranyadavR @Arunan & others.

Theertha · June 13, 2024, 12:25pm

Graph made using observations over three days of fruit flies on tomato slices.
What do you say ?@⁨Rahul CUBE⁩ @⁨Batul Ma’am⁩ @⁨Aastha⁩ @⁨Himanshu Joshi⁩ @⁨Smiti⁩ & others.
Where did these flies went at night,for sleeping?

Related Graph made by Cubists:

Observations by @saswathy679

observations by @_Seema_11

Observations by @Rahul

IMG-20240607-WA0000(3)

[07/06, 7:02 am] Rahul CUBE: Some results from my own experiments suggesting that drosophila has sexual dimorphism in their sleep patterns. Many cubists have been studying the activity patterns in drosophila. Can someone see some connection between the two? @⁨Arunan sir ⁩, @⁨Theertha⁩, @⁨Abhijeet CUBE⁩ ??

[07/06, 8:01 am] Rahul CUBE: Sorry, I forgot to clarify one thing regarding the X-axis here. It is not the time of the clock but the zeitegeber duration (light/dark). So, 0 on the X-axis is 9am and 12 on the X-axis is 9pm

[07/06, 8:27 pm] Rahul CUBE: They are active most of the time. But this graph tells you the pattern of activity and sleep when monitoring individual flies. You’re right they are mostly more active and not sleeping around dusk and dawn. And the general trend is that both males and females spend a higher proportion of time being active during the day and females sleep higher during the night compared to males.
[07/06, 8:30 pm] Rahul CUBE: @⁨Batul Ma’am⁩ , @⁨Theertha⁩ Another interesting thing to notice is that female flies have a sleep-wake pattern similar to humans. What do you think?

Theertha · June 14, 2024, 3:24pm

Phyllanthus urinaria leaf folding movements:

Objective: To observe leaf folding movements in Phyllanthus urinaria under continuous darkness.
Hypothesis: Plants kept in continuous darkness will show delayed leaf closing and early leaf opening.

Setup:
Four Phyllanthus urinaria plants were collected on 27/05/2024.
Complete leaf opening was observed at 7 am, and complete leaf closing occurred at 6.30 pm on 6/06/2024.
On 06/06/2024 at 6:33 pm, two Phyllanthus urinaria plants were placed in a plastic bucket and covered. Two of them were placed in the shaded region.
Date of photo:30/05/2024, Cherukkad, Theertha.

Summary of the observations:
6/08/2024:Sunrise :6:03 am Sunset :6:48 pm.
leaves completely opened at 7 am and closed at 6.30pm.

Kept 2 Phyllanthus plant on sunshade and 2 in complete dark at 6.33pm on 6/08/2024.
7/06/2024 DAY:1
Sunrise :6:02 am Sunset :6:49 pm
Sunshade plant :
Leaf complete opening:7am

Leaf complete Closing:5.24pm

Dark plant:

Leaf complete opening:7am

Leaf complete Closing:5.24pm

8/06/2024.DAY:2
Sunrise :6:03 am Sunset :6:49 pm.
Sunshade plant :

Leaf complete opening:7am

Leaf complete Closing:6.30pm
Dark room plant:

Leaf complete opening:9.52am

Uploading: IMG_20240608_180112.jpg…

Uploading: IMG_20240608_202001.jpg…

Leaf complete Closing: Partial leaf closing .(10.55pm).

9/06/2024:DAY:3
Sunrise :6:03 am Sunset :6:50pm
Sunshade plant :

Leaf complete opening:7am

Leaf complete Closing:6.30pm

Dark room plant

Leaf complete opening:11.40am.

Leaf complete Closing:Partial leaf closing 9.44pm.
Day 4
10/06/2024
Sunrise :6:03 am Sunset :6:50pm
Sunshade plant :

Leaf complete opening:7.30am

Leaf complete Closing:6.38pm
Dark room plant

Leaf complete opening: 4.15 pm (photo taken after 7am).

Leaf complete Closing:Partial :11pm.
DAY:5
11/06/2024
Sunrise :6:03 am Sunset :6:50pm
Sunshade plant :

Leaf complete opening:8am

Leaf complete Closing:6.10pm
Dark room plant

Leaf complete opening:12pm.

Leaf complete Closing:Partial closing 11.39pm.

Summary
Leaves are not completely closed after the second day, and delayed leaf opening and closing were observed. Also Leaves are detaching from the main stem.

Conclusion: Phyllanthus does have an internal clock that repeats every day. But that rhythm is synchronized with light,so in the absence of light, the leaf opening and closing rhythm changes.

Theertha · June 14, 2024, 3:28pm

Summary:

Phyllanthus urinaria leaf folding movements:
Objective: To observe leaf folding movements in Phyllanthus urinaria under continuous darkness.
Hypothesis: Plants kept in continuous darkness will show delayed leaf closing and early leaf opening.

Summary of the observations:
6/08/2024:Sunrise :6:03 am Sunset :6:48 pm.
leaves completely opened at 7 am and closed at 6.30pm.

Kept 2 Phyllanthus plant on sunshade and 2 in complete dark at 6.33pm on 6/08/2024.
7/06/2024 DAY:1
Sunrise :6:02 am Sunset :6:49 pm
Sunshade plant :
Leaf complete opening:7am
Leaf complete Closing:5.24pm

Dark plant:
Leaf complete opening:7am
Leaf complete Closing:5.24pm

8/06/2024.DAY:2
Sunrise :6:03 am Sunset :6:49 pm.
Sunshade plant :
Leaf complete opening:7am
Leaf complete Closing:6.30pm
Dark plant:
Leaf complete opening:9.52am
Leaf complete Closing: Partial leaf closing .(10.55pm).

9/06/2024:DAY:3
Sunrise :6:03 am Sunset :6:50pm
Sunshade plant :
Leaf complete opening:7am
Leaf complete Closing:6.30pm

Dark room plant
Leaf complete opening:11.40am.
Leaf complete Closing:Partial leaf closing 9.44pm.
Day 4
10/06/2024
Sunrise :6:03 am Sunset :6:50pm
Sunshade plant :
Leaf complete opening:7.30am
Leaf complete Closing:6.38pm
Dark room plant
Leaf complete opening: 4.15 pm (photo taken after 7am)
Leaf complete Closing:Partial :11pm.
DAY:5
11/06/2024
Sunrise :6:03 am Sunset :6:50pm
Sunshade plant :
Leaf complete opening:8am
Leaf complete Closing:6.10pm
Dark room plant
Leaf complete opening:12pm.
Leaf complete Closing:Partial closing 11.39pm

Interpretation:
Leaves are not completely closed after the second day.
And delayed leaf opening and closing were observed.
Leaves are detaching from the main stem.

Conclusion:
Phyllanthus do have a internal clock that repeat s everyday.
But that rhythm is synchronised with light that’s why in the absence of light leaf opening and closing rhythm got changed.