Long term:- GUSTATORY RESPONSE (role of chemoreceptors)
Short term :- behavior towards salt…
CUBE HBCSE,MUMBAI
EARTHWORM group
Date:07/09/19
@Riya
@Vishal
Plan of work
1.performing experiment on gustatory response (role of chemoreceptors)
2.identification of species of EARTHWORM which were trapped in hbcse garden…(10earthworm)…
How are you planning to perform gustatory response assay with Earthworms? What is design of an experiment?
How will you identify earthworms? What are salient characteristics of Eiseina feitida a well known earthworm species?
The design for gustatory response is
Taking a glass sheet with few strips of filter paper and making salt solution of different concentration for example (100mM,200mM,300mM)
Then placing the filter paper adjacent to each other with distance of 5mm
Then one side is moist with distilled water and paper with salt concentration and then place the earthworm in the passage between the two …
Special precaution … see that there is no solution in the passage and remember or label the sides where the salt conc is present or the DISTILLED WATER…
Why the concentration is only selected as 100,200 or 300 mM NaCl? What is the rationale behind selecting this? Why only distilled water? Don’t you think distilled water will provide more stress to the earthworm? @Rupesh @Hrushikesh
@Rupesh What conclusion did you draw of the role of chemoreceptors after performing the Gustatory Response Assay?
How many times did you perform this?
What were your observations after each run?
How much was the cut-off time given to the organism?
Have you identified your species? If yes, what characteristics did you observe in yours organisms… like the average length, the number of eggs layed and so on.
See, these and many other…are the questions that I feel are needed to be answered for you to get a clear base understanding of your GUSTATORY RESPONSE…
Till then, keep posting!!
@drishtantmkawale
From the assay we (@RiyaChari, Vishal ) concluded that an native earthworm can bare upto 200 mM Nacl concentration
So we have performed this assay for once only with the native ones …
For each concentration of Nacl we take 30 runs
We have 4 concentration
1 . Control (distilled water vs distilled water)
2 . 100 mM ( distilled water vs 100mM Nacl)
3 . 200 mM ( distilled water vs 200mM Nacl )
4 . 300 mM ( distilled water vs 300mM Nacl )
After each run I observed that the earthworm moved towards the salt till 200 mM Nacl but it repelled at 300 mM Nacl and moved towards distilled water
The cut off time of each run given was 2 minutes
Now for further gustatory response we will use eseinia fetida and see whether there is some changes or all earthworm are the same …
Till then stay calm and turn on ur notification 
As per my knowledge we calculated that 100 mM Nacl is a hypotonic solution
2. 200 mM Nacl is isotonic solution
3. 300mM Nacl is hypertonic solution
For the earthworm
So we decided to use the same amount of concentration for the assay
No I don’t think so it will provide any stress but by the academics I think it will produce some stress as the distilled water contains no ions may be the salt in the body may move from higher concentration to the lower concentration
Need some more time to study…
So keep ur fingers crossed. Till further updates 
“see whether there is some changes or all earthworm are the same”
What changes are you expecting in the earthworms?
Are those changes morphological, anatomical or physiological?
Or all three?
Do you have any cross references to refer to?
Also, photo and video references are essential to create a context and visualise the same!!
Looking forward for photo and video updates
So do you mean to say that the water/solute from the body of the earthworm would diffuse in/out?
If some earthworm stays near coastal area
May be will have a high potential of salt tolerance…
Or different species from different places may have different tolerance level
Not all earthworm are same some are find on the surface
So, where is Earthworm species Eisenia fetida normally found?
From where are you going to get these worms?
Will it be from the coastal area?
Eisenia fetida are Commonly found under the logs of wood or under some other things…
The native one which we found was clearly seen on the soil 
So have you distinguished between the native earthworms and Eisenia fetida?
Or I must ask, have you tried to identify and characterise your native earthworm species?
Why not try finding the Eisenia fetida as you mentioned, under the logs of wood or under some other things like under the flower pots?
Each and every try was given to find them while the Christmas vacation cube workshop due to the dry conditions no earthworm was found but
We got eseinia fetida from our seniors so we will perform the assay soon . Stay connected to the page for further information…