Hydra: A Powerful model for regenerative study

[Theertha (Theertha M.D.)]

There was an interesting story by @sakshiconsultant2002
On 27/7/2022 she made a single line culture for one hydra ,later on 22/8/2022 that became 19 hydras .

Then the disscussion turned into the structure of hydra .
In this picture we can see a enlarged portion of gastric region , that is divided into
1)Outermost layer:Ectoderm
2)Middle :Mesogloea
3)Inner :Endoderm

Then we noticed that the Stem cells is distributed evenly in body column so that is helpful for the regeneration of hydra .
The previous experimental results are also showing that , if we cut the hydra in to three 1) head portion 2) middle portion and 3) tail portion each of them will regenerate because of this stem cells .

https://www.researchgate.net/figure/Anatomy-of-a-hydrozoan-polyp-AA-Hydra-polyp-is-essentially-a-two-layered-tube-with-a_fig3_50351101

Yesterday the discussion was started with Hydra initiated by @Sakshi
The Ratnam college group received Hydra and Moina from @dhanraj.tribhuvan
@sakshi @Archita15 made a setup for observing feeding habit of Hydra .@dhanraj told them that ,one moina is enough to feed for one hydra .
@Sakshi made a experimental setup that include 1:3 ratio for feeding .For 1 hydra 3 moina were added to the bottle .

The set up include :
Two seperate plastic container with 1:3 ratio of feeding ,marked as experiment and replicate .
One control is with 1:1 ratio (Dhanraj formula )for feeding .

For this experimental setup three plastic container were used .Also made some holes on the top of the aluminium foil for aeration .

The plastic container with 15 ml of dechlorinated water was used .one Container with 3 hydra each .

Then the disscussion was about the red moina . that, more than one drop of a milk make moina red .
This cause the increasing in the number of bacteria there by decrease the amount of oxygen .
In order to cope up this ,Moina will produce more hemoglobin .

[jaikishan]

Hydra has STEM cells?
Can we culture the STEM cells from hydra?
Can hydra serve as a model for studying STEM cells?

[Theertha (Theertha M.D.)]

Yes,Hydra have Stemcells.

Hydra is a stem cell model system for aging research and regenerative biology and exhibits an almost complete metazoan-specific molecular toolkit.

Our picture of Hydra stem cell biology has changed in that there are four stem cell populations i.e., ectodermal and epithelial stem cells, interstitial somatic multipotent stem cells (MPSC) and interstitial germline stem cells (GSC).

The Hydra stem cell system – Revisited

How can we Culture those Stem cells? @jaikishan @sakshiconsultant2002 .Like Plant tissue cultue,do they(Animal cells) have media to culture?

[Theertha (Theertha M.D.)]

Chatshaala discussion:30/12/2024
Summary written by @Enas_188

Hydra / Chlorohydra has capacity to regenerate its full body when they cut into pieces.

What happens to the cells at molecular level of the chlorohydra when they cut into pieces?

What is hypoxia inducible factor?
How HIF is connected with moina, people at higher altitude and Chlorohydra?

What is the size of moina, Fruifly, hydra? How can we explain it using ruler?

Sailekshmi has got some moina like organism in her college pond. Sailekshmi let’s culture them in plastic bottle containing water and adding 1 drop of milk.

Reference shared:

Hypoxia-Inducible Factor (HIF)-1 Regulatory Pathway and its Potential for...

https://www.sciencedirect.com/topics/neuroscience/hypoxia-inducible-factors#:~:text=Hypoxia-inducible%20factors%20(HIFs),angiogenesis%2C%20metabolism%2C%20and%20invasion

Hypoxia-Inducible Factor (HIF)-1 is a dimeric protein complex that plays an integral role in the body’s response to low oxygen concentrations, or hypoxia. HIF-1 is among the primary genes involved in the homeostatic process, which can increase vascularization in hypoxic areas such as localized ischemia and tumors. It is a transcription factor for dozens of target genes; HIF-1 is also essential for immunological responses and is a crucial physiological regulator of homeostasis, vascularization, and anaerobic metabolism. Furthermore, HIF-1 is increasingly studied because of its perceived therapeutic potential. As it causes angiogenesis, enhancement of this gene within ischemic patients could promote the vessel proliferation needed for oxygenation. In contrast, as HIF-1 allows for survival and proliferation of cancerous cells due to its angiogenic properties, inhibition potentially could prevent the spread of cancer. With a growing understanding of the HIF-1 pathway, the inhibition and stimulation of its transcriptional activity via small molecules is now an attractive goal. Gene therapy to achieve both vessel proliferation and tumor regression has been demonstrated in animal studies but requires significant improvement and modification before becoming commercially available. This review focuses on the potential of the HIF-1 pathway in therapeutic intervention for the treatment of diseases such as cancer and ischemia.

why people living in mountains have more red corpuscles in thier blood than...

What is the amount of oxygen o2 dissolved in water? The oxygen content of surface waters of normal salinity in the summer is typically more than 8 milligrams per liter (8 mg/L); when oxygen concentrations are less than 2 mg/L, the water is defined as hypoxic (CENR, 2000). https://www.usgs.gov › science › di… Dissolved Oxygen and Water | U.S. Geological Survey - USGS.gov

[Theertha (Theertha M.D.)]

CUBE CHATSHAALA:25/12/2024

Control
Photo:Sakshi

0.1mM
Photo:Sakshi

0.2mM
Photo:Sakshi

0.3mM
Photo:Sakshi

CUBE CHATSHAALA:25/12/2024

Sakshi is interested to Investigating the role of hypoxia inducible factor HIF in regeneration of hydra using cobalt chloride as a hif inhibitor.

The discussion was about the Chlorohydra and the inhibition of HIF. In order to inhibit HIF, Cobalt chloride used.

Photo :Sakshi @sakshiconsultant2002

Reference shared:

The use of cobalt chloride as a chemical hypoxia model - PubMed