CUBE Nerul, Navi Mumbai, Home lab
Update and Plan of Work 5th June 2020
Breaking News!!
The greenish growth is flourishing!! 
The first instance of observing the greenish growth in the two bottles Y1 and Y2 was on 28th of May, 2020.
Since then till yesterday i.e. till 4th of June, 2020 the growth has been increasing.
I suspect 
this greenish growth to be algae.
Why?
I can provide the following information:
- The greenish *growth has been increasing day by day.
- As I am keeping the two bottles in the window to get sunlight (not direct sunlight), the growth is seen to be increasing, it shall be a photosynthetic algae which is sustaining in the water.
- Here, I had taken Dechlorinated Water , which is freshwater . So, the algae surviving in that shall be a freshwater photosynthetic algae.
Further plan:
To introduce Moinas into the two bottles having suspected-to-be- algae.
Moinas are found in freshwater bodies like ponds, lakes and shallow streams where they feed on Algae, Bacteria, Fungi and decaying matter.
Keeping this is mind, when the Moinas will be put in these bottles having 500mL of dechlorinated water + greenish growth, I expect them to sustain/survive in it.
I have observed the same greenish growth in one of the Moinas tanks in CUBE HBCSE, Mumbai where Moinas were surviving without adding any external source of food such bacteria.
It will be mimicking their natural ecosystem!
As this too is an experiment, it shall have a defined control as well as test, both with replicas.
Control will show that the Moinas will survive definitely.
In Test, I will be checking (by adding Moinas) whether the survive or not.
I was planning to prepare 5 stock culture bottles with 500mL of dechlorinated water + 2 drops of milk + ~10 Moinas in each bottle.
And also, my control will consist of the same. So, I need not make a separate control for the experiment. The stock bottles will act as a control.
The test, two bottles Y1 and Y2 having 500mL of dechlorinated water + ~10 Moinas + greenish growth (assumed to be algae) inside the bottle as the feed.
Why only two bottles for test?
Actually, this was a spontaneous observation of greenish growth (I didn’t expect that). So, I thought that I should introduce some Moinas in them and see if they survive by feeding on algae. So, just two bottles have this greenish growth.
I do not know how do Moinas feed on algae (but they are alive). So everyone’s contribution is needed in finding that out.
Note: From the start, I have five Moina stock culture bottles fed with milk. So, when their growth curve starts coming to an end, I gather all the Moinas in a cup and then wash the bottles with tap water, then I prepare a new culture media for them. It consists of 500mL of DC Water + 2 drops of milk (in the bottles), later in which I add Moinas.
So right now, I have around 100 Moinas as my stock.
The above pictures show the increase in the greenish growth from 28th of May, 2020 to 4th of June, 2020, in the bottles Y1 and Y2, both having 500mL of dechlorinated water and are kept in the window to get sunlight (not direct sunlight).
Date of Pictures: 28th May 2020 to 4th June 2020
Data Collector: Drishtant MK.