How moina can be introduced to Students at Azim Premji University, bhopal? Through demonstration? Through past experiences of searching something (moina) and getting something else (hydra) as Rechel has got !!
What should be the design for demonstration? @Sakshi @Rechel CUBE
Rechel observation of hydra sticking to the wall of the bottle.
How fruitflies can be introduced?
Post made by @sakshiconsultant2002 in Context to curriculum whatsapp group
[17/01, 3:14 pm] Sakshi: 
Hello all,
Today I will be taking chlorohydra and moina culture to my home lab .
So that we will be able to get better idea for Azim Premji University workshop
I will be taking 2 falcon tubes in which there will be 50 moinas each. And they will be labelled as M.C 1 and M.C2.
Then I will take 2 more falcon tubes in which there will be chlorohydra culture.
In each 2 tubes there will be 30 hydras. And labelled as H.C1 and H.C2.
[17/01, 3:35 pm] Sakshi:
This is labelled moina culture kept in falcon tubes of 50 ml approx.
Moinas kept in dechlorinated water.
[17/01, 3:37 pm] Sakshi:
Video of moina culture
[17/01, 3:39 pm] Sakshi:
This is hydra culture labelled with HC1 and HC2.
[20/01, 10:42 pm] Sakshi: Hello everyone!
This is C.U.B.E homelab culture of chlorohydra and moina.
The 2 Bisleri bottles of approximately 250 ml contains moina culture with around 50 moinas in each.
The 2 glass below contains chlorohydra culture where there are approximately 30 chlorohydra in each.
These cultures were brought in 4 falcon tubes on 18th January 2025.
And the culture was transferred in the bottle and glass on 19th January 2025.
[20/01, 10:45 pm] Sakshi: This is the closeup picture of chlorohydra.
This chlorohydra was fed on 18th January 2025.
On 19th the chlorohydra were not fed.
[20/01, 10:45 pm] Sakshi: This is how the culture looks like.
Picture taken on- 20th January 2025
[20/01, 10:46 pm] Sakshi: Each glass of chlorohydra contain approximately 30 chlorohydras.
[20/01, 10:47 pm] Sakshi: This is the video of moina culture!
Moinas are accumulated at the bottom where there is source of light.
Summary written by @Enas_188
What will the status of Mango Trees in Bhopal when we will go for Workshop at Azim Premji University?
When is the RIPE Magoes available in the Market? Can we predict the flowering from this information?
[17/01, 9:35 pm] Enas CUBE: Mobile FT mRNA contributes to the systemic florigen signalling in floral induction
Chunyang Li, Mei Gu, Nongnong Shi, Hang Zhang, Xin Yang, Toba Osman, Yule Liu, Huizhong Wang, Manu Vatish, Stephen Jackson & Yiguo Hong Mobile FT mRNA contributes to the systemic florigen signalling in floral induction | Scientific Reports.
[17/01, 9:46 pm] Arunan sir : Florigen signaling
Hiroyuki Tsuji 1, Ken-Ichiro Taoka 2
Affiliations Expand
PMID: 25740717 DOI: 10.1016/B978-0-12-801922-1.00005-1
Abstract
Florigen is a systemic signal that promotes flowering. Its molecular nature is a conserved FLOWERING LOCUS T (FT) protein that belongs to the PEBP family. FT is expressed in the leaf phloem and transported to the shoot apical meristem where it initiates floral transition. In the cells of the meristem, FT binds 14-3-3 proteins and bZIP transcription factor FD to form the florigen activation complex, FAC, which activates floral meristem identity genes such as AP1. The FAC model provides molecular basis for multiple functions of FT beyond flowering through changes of its partners and transcriptional targets. The surface of FT protein includes several regions essential for transport and functions, suggesting the binding of additional components that support its function. FT expression is under photoperiodic control, involving a conserved GIGANTEA-CONSTANS-FT regulatory module with species-specific modifications that contribute variations of flowering time in natural populations. Florigen signaling - PubMed
[18/01, 4:37 pm] Enas CUBE: How’s Mango Trees Flower?
Is there a connection between formation of New leaf with Flowering?
How Mango Trees flowers? What does farmers spray to the Mango to initiate Flowering?
What is Meristem ? How shoot apical Meristem (giving new leaf) converts to a Meristem which gives Flower?
[18/01, 4:37 pm] Enas CUBE: What is the role of Florigen in Mango Flowering?
[18/01, 4:38 pm] Enas CUBE: @Theertha @Arunan sir @Rechel CUBE @Chithra Ma’am @~Batul Pipewala
CUBE whatsapp group discussions:
[18/01, 6:43 pm] Batul: Suppose we divide the 200sqm of area into 10squares of 20 square metre each and take observations of atleast 2mango trees in each grid.
We will have a large representative sample of 20mango trees.@Dr Arunan @~Seethalakshmi✨️ and others
[18/01, 6:44 pm] + Batul: @Enas CUBE @Theertha and others
[18/01, 6:50 pm] Arunan sir 
: How do we apply this scheme in Mango Tree Mapping for flowering?
Do you think, we can reproduce this scheme;in the same way, in the case of Mango Flower mapping in Bhopal? @~Batul Pipewala @Enas CUBE @Seethalakhmi @Theertha and others
Summary written by @Enas_188
[20/01, 6:10 pm] Enas CUBE: How do we isolate DNA from cell? Basic mechanism of doing each step? What is the role of crushing the fruit, adding detergent, salt?
20/01, 6:14 pm] Enas CUBE: How are 3 barrier (cell wall, cell membrane, nuclear membrane) in plant is broken? When we add salt what happens to the protein, lipid and DNA present in the solution?!
Summary written by Akansha
Summary 21/1/2025 What different types of kitchen waste like potato peel, tomato slice, onion slices roots, banana peel tea powder etc attract the fruit flies in our home in the kitchen and also outside the home near windows or in the balcony. So we can perform food preferences tests by keeping different food waste at particular distance on paper and check to which fruit/vegetables waste the drosophila are attracted to most. So from this topic of kitchen waste we related it to neuroscience behind the smell how flies smell the waste through diffused molecules and then bind to receptors specific sending signals to perceive the smell in the brain in olfactory bulb region and get attracted towards it.
TRSV media bottle prepared today 22/1/2025 to culture the flies trapped on 21/1/2025
TRSV medium in a glass bottle at Homelab Kolkata Batul
Banana waste and tea powder attracting fruit flies in the kitchen waste 22/1/2025
Fruit flies trapped in a glass jar by keeping a banana peel inside the jar kept outside the house in the balcony
21/1/2025
Akansha
Photo: Batul
Food preferences in fruitflies
Fruit fly on random kitchen waste resources prefer some more than others, Mumbai, 20 January 2025 Photo mcarunan
