How genetic engineering in cardamine plant by floral dip method connect to the vast biological context to Antibiotic action, Basic Biochemistry, Basic Cell Biology, Evolution, Molecular Biology, Drug action, Types of Drugs and so on…
It also shows up gaps in our understanding as well as about several misconceptions, all getting clarified slowly through collaborative learning!
Today Discussed about Nazish great finding Germination and growth study’s of cardamine seeds in the presence of kanamycin. Using different concentration kanamycin solutions and two control using tap water (with out using kanamycin solution). She got germination in all 6 set up. Using kanamycin and control test also . 85% - 90% Germination growth get in her experiment. Interesting part was while using kanamycin solutions at different concentration seedling growth shows decreasing and also the leaf color are slightly changed Green to Yellow ! Why!!?
What is the major reason while using kanamycin at different concentration shows became effect the growth of plant and changes the color in green to yellow ! Why?
Kanamycin is an antibiotics. Applied in plant to save from bacteria . Bacteria eukaryotic and plant cell is Prokaryotes While using kanamycin it will effect to ribosomes which are present in Mitochondria and Chloroplast which are contains 70s Ribosomes in plant cell. And also Bacteria is contains 70s Ribosomes. While applying kanamycin antibiotics in to plant they became stop the production of protein synthesis. So Chloroplast contains chlorophyll and it’s main function to photosynthesis and give green color to plant leafs. While stop producing proteins in chloroplasts leave color will change Green to Yellow . Mitochondria function is to production of ATP .While stop producing ATP and energy that will effect to plant growth . Growth will decreases.
So that’s why we can clearly see nazish experiment the growth of plant will going to decreases and also chage the color difference green to yellow .
What is the major reason while using kanamycin at different concentration shows became effect the growth of plant and changes the color in green to yellow ! Why?