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Timing:5.30 pm to 9.30 pm
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336th day of CUBE ChatShaala: 18th February 2021
28 CUBists from 24 Centres had joined the webinar
Map
ANDHRA PRADESH
Nellore: Anuhya Mucheli, Sri Ranjini, Tejasri B, Ujwalaraj
ASSAM
Jorhat: Ishita Sonowal
DELHI
Aastha Ahuja
Dyal Singh College: Dr P. Chitralekha
Raj Yashwantrao Patil
JHARKHAND
Morabadi, Ranchi: Rechel Tirkey
KERALA
Kozhikode: Arunima
Kandassankadavu: Lakshmy PJ
SN College, Nattika: Seethalakshmi CR
Thanniam, Thrissur: Sidhy PP
MAHARASHTRA
South Bombay: Arunan MC
Nerul: Drishtant M Kawale
Worli, Mumbai: Isha Pawle
HBCSE, Mumbai: Kiran Yadav
Powai: Lydia Mathew
Mulund, Mumbai: Priti Kanade
Dahanu, Palghar: Sachin Pradhan
Bandra, Mumbai: Saida Sayyed
Panvel, Navi Mumbai: Shraddha Sonavane
Ambernath: Shruti Choubey, Sonu Choubey
Shivaji Nagar, Govandi: Tanveer Khan
WEST BENGAL
Kolkata: Batul Pipewala
Shruti Kandula
Omkar Wakade
Cube ChatShaala 18th Feb 2021
Summary
The discussion began with Ishita sharing about her Model Organism, Zebrafish. Zebrafish is a leading Model Organism for studying Immunology because of the Immune responses in early embryonic stages, since the eggs are transparent, was stated by Ishita. Zebrafishes being easy to maintain. Soo we asked Raj if he could start a Zebra fish culture as it is also related to Immunology. Soo where do we find the zebrafish? They can be found in Fresh Water Streams and if those are not available they are readily available in Fish Aquariums.
How to maintain and culture the Zebra Fishes? (Without Air Pump)
• Take a 1 L container.
• Add 750 ML of declorinated water.
(How to Make Declorinated Water? Take water inside a 1L container and cover it over using a cloth and keep it overnight, you will get declorinated water)
• Take the Plastic Bag in which the Zebrafish were given. (From aquariums)
• Place the Plastic Bag into the water for an hour or two.
(Why we playing the Plastic Bag for an hour or two? Fish can get used to the Tank Temperature, if we directly add the fish to tank water it might die from sudden temperature shock)
• Add the Zebra Fishes to the 1 L container.
• Change the Water on every 3rd day.
(How to change the water? Take 200 ML of water with fish from the tank and separate it and change the water inside tank and add the separated 200 ML of Water which has fish inside it)
• The Zebra Fish should be fed using Moinas/Micropellet. ( 5 Moina per Fish and 2 Micropellet per fish ) [We were not sure about Moina]
Soo where to find Moinas? How to culture them? Moinas are found in Fresh Water Ponds.
How to Culture Moinas
• Take a 250 ML bottle.
• Add declorinated water to the bottle.
• Add Moinas to the Bottle.
• Add a single drop of milk which will help the Bacterias grow.
• Moina will feed on Bacterias.
Ishita shared Zebrafishes don’t overfeed. So can we culture Moinas in the same Zebrafish Container??
We moved ahead and talked about Innate Immune System which Ishita wants to study about.
Soo what exactly is Innate Immune System? Ishita answered that Innate Immune Response is what we call Inflammation.
What is Inflammation? What is Histamine? Ishita shared about one of the Immune Cells which are called as Macrophages.
What do Macrophages do? Macrophages engulf and destroy the foreign cells. We tried to understand how Macrophages reach the site of infection/injury. At the site of injury/infection the cells send signals to the immune cells which results in Immune cells reaching the site of infection/injury.
Soo how exactly do the Macrophages move? Ranjini Ma’am explained that Macrophages are present in every part of the body and they have Amoeboid movement. Ranjini Ma’am introduced us to her PhD question which was related to Cypermethrin. (Sorry I too don’t understand it)
We further talked about Ishitas Zebrafish condition and Zebrafish Breeding Setup which included a Container, Dish and Net as shown on white board.
Later we continued with Immune Response. What is Agglutination? We continued discussing about Agglutination according to Raj, agglutination is the first immune response in which the immune cells bind to the foreign cell. Soo does Agglutination always take place? Agglutination is followed by Opsonination. Where the Immune Cell binded Forgein Cell is phagoctysed by the macrophages. Opsonination is followed by Lysis. Where the phagoctysed forgein cell is broken down into smaller molecules.