S3E24 Cube chatShaala: Exploring new ways of Learning

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Timing:5.30 pm to 9.30 pm
Webinar will be recorded and recording link will be post in the same thread after the webinar.

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CUBE ChatShaala on 2nd December 2020
32 CUBists from 27 Centres joined the webinar.
Map

ANDHRA PRADESH:
Nellore: Anuhya Mucheli

ASSAM:
Narsing High School, Silchar: Hasina Begam
Jorhat: Ishita Sonowal
Sonari: Susanti Tanti

BIHAR:
Sitamarhi: Anamika Singh

KERALA
Ernakulam: Aishwarya Elizabeth, Deepthy Maria, Devika Devanath, Essabell Philomina, Rexeena Sebastian
Thriprayar, Thrissur: Aswathy Suresh
Aroor: Pooja Baiju
Thanniam, Thrissur: Sidhy PP

MAHARASHTRA:
South Bombay: Arunan MC
Ghatkopar, Mumbai: Anjanikumar Kashyap
Shirpur, Dhule: Aryan Nitin Patil
Nerul: DK
HBCSE, Mumbai: DP
Powai: Lydia Mathew
Mumbai: Neelam Bhattarai
Mulund, Omkar Badnale
Dahanu, Palghar: Sachin Pradhan
Bandra, Mumbai: Saida Sayyed

TAMIL NADU:
Chennai: Anjali Chauhan

UTTAR PRADESH:
Meerut: Chirag Pulwani
Kanpur: Hina Mudgal
Moradabad: Kiran Yadav

WEST BENGAL:
Garden High School, Kalyani: Aranyak Sarma
Kolkata: Batul Pipewala, Sukriti

Anusree M
Mohamed Faraz

Quoted by @saswathy679
IMG-20201203-WA0001
2 December 2020
[CUBE Chatshaala]

Single Line Cultures
Bottukadavu Thrissur Kerala.

SLBK A, SLBK B, SLBK C, SLBK E, SLBK F
5 Single Line Cultures

SLBK - Single Line Bottukadavu Kerala.

2/12/2020 - Stock Cultures are SLBK A8, SLBK B8, SLBK C8, SLBK E4, SLBK F4 which I made up on 25/10/2020. 1 months & 5 days old culture bottles.

Research question need to first trap fruit flies and culture single line/ pure lines by catching gravid females and identify Native Drosophila melanogaster amongst the native fruit flies from the environment!

Research objectives

Immediate objective
To make Home made media for culturing Native Fruit flies.
To create multiple Single Line culture of Native fruit flies by keeping a bait near kitchen garbage using tomato slice/banana peel and catching gravid females and creating Native Single lines.

Short term Objective
To identify native single culture of fruit flies as Native Drosophila Melanogaster by looking at morphological characteristics relevant to Family Drosophilidae, Genus Drosophila and Species melanogaster

Long term objective
To perform olfactory assay at range of concentrations of IAA (banana essense)
using standard CsBz 2nd instar larvae and Native Drosophila melanogaster 2nd instar larvae and compare their olfactory sensitivity to comment on evolutionary changes in terms of fruit odor smelling

Today situation observe still Flies are living in that bottles and Also observed some unhatched pupae, ~15-20 larvae are present in the Culture Bottles.

To make a plan of work to do today & tomorrow onwards - How to save the culture of one particular one single species which are not identified further Species level.

HOME LABS
Fruit Flies Collaborator at different parts of country including me also :-
1.Arunan sir - Mumbai
2.Batul ma’am - Kolkatha
3.Hina ma’am - Kanpur
4.Kiran ma’am - U P
5.Yash - Prabadavei,Mumbai
6.Lydia - Mumbai
7.Isha - Mumbai
8.Deepika - Mumbai
9.Khipra - Damka, Faradhabad
10.Sidhy - Thannyam Thrissur Kerala
11.Man Mansih - Ranchi
12.Manjual - Ranchi
13.Saida - Mumbai
14.Drishtant - Navi Mumbai
IMG-20201203-WA0002
2 December 2020
[CUBE Chatshaala]

PLAN OF WORK

SLBK A8 - 25/10/2020
1 month & 5 days old bottles

2 December 2020 : Observation
•~10-15 adult flies
•~15-20 different stage of larave
•~20-25 unhatched pupae

3 December 2020 : Plan to Do
•Transfer flies from new TRSV media Bottles
Media Prepared on 2 December for whole one day solidification.
•Transfer flies from SLBK A8 - SLBK A9 (New)
•~6 Gravid Flies, ~4 Male Flies = ~10 Flies.

Expectations

  1. 3/12/2020 - Gravid Flies Laying Eggs (0 Day)
  2. 6/12/2020 - First Visible Larvae (3 Day)
  3. 7/12/2020 - First Pupae (4 Day)
  4. 11/12/2020 - New Generation Flies (8 Day)

IMG_20201202_213942