S3E36 Cube chatShaala: Exploring new ways of Learning

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Timing:5.30 pm to 9.30 pm
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CUBE ChatShaala on 14th December 2020
39 CUBists from 32 Centres had joined the webinar.
Map

ANDHRA PRADESH
Nellore: Tejasree, Ujwalaraj

ASSAM
Sonari: Susanta Tanti

BIHAR
Sitamarhi: Aryan Kumar

DADRA AND NAGAR HAVELI
Silvassa: Jeene Jimmy, Saumya Jimmy

DELHI
AND College: Dr Sarita Kumar
DDU College: Dr Sudhir Verma

JHARKHAND
Kokar, Ranchi: Man Masih Beck
Ranchi: Manisha Rani, Suraj Kumar
Morabadi, Ranchi: Rechel Tirkey, Dr Shalini Lal

KERALA
Muvattupuzha: Abhijith Vinod
Kozhikode: Arunima
Thriprayar, Thrissur: Aswathy Suresh
Ernakulam: Devika Devanath, Rexeena Sebastian
Palakkad: Shyam TG
Thanniam, Thrissur: Sidhy PP

MAHARASHTRA
South Bombay: Arunan MC
Ghatkopar, Mumbai: Aashutosh Mule, Anjanikumar Kashyap
Nerul: Drishtant M Kawale
Powai: Lydia Mathew
Andheri, Mumbai: Mandar Chavan
Byculla, Mumbai: Nazish Kaunchale
Thane: Prashant Palane
Bandra, Mumbai: Saida Sayyed
Panvel, Navi Mumbai: Shraddha Sonavane
Murbad, Kalyan: Shubham Isame
Prabhadevi, Mumbai: Yash Sheregare

TAMIL NADU
Chennai: Anjali Chauhan, Anu Kiruthika

UTTAR PRADESH
Kanpur: Hina Mudgal
Moradabad: Kiran Yadav

WEST BENGAL
Garden High School, Kalyani: Aranyak Sarma
Kolkata: Batul Pipewala

Unknown Affiliation
Rahul

Quoted by @saswathy679
IMG-20201214-WA0068
14 December 2020 CUBE Chatshaala.

Discussed about Model System CARDAMINE PLANT. from panvel @Shraddha got many cardamine plants at near her home (first week of October 2020). 6 Cardamine she brought and transplant in to native soil in Mumbai. In Panvel have 2 pot culture of cardamine. First one she transplanted & second one collected seeds from pot 1st and sowing in to soil directly.

1.Why cardamine is taken as a model system🤔?
Cardamine is in the family of Brassicasia which are belongs to Arabidopsis plant. Arabidopsis plant is the powerful model system in plants. Genetic studys are done in this plant. But this plant not seen in our India. It’s more visible at low temperature countries.
Cardamine are easily available at everywhere in India. So that’s why cardamine taken as a model system in the place of Arabidopsis plant.
IMG-20201214-WA0069

In CUBE, lots of Cardamine Collaborators spread over the india now.
In Ranchi people done Genetic studies in this cardamine flower buds. They done florals dip method & produced Transformed seeds from that genetic studies. But they didn’t test control Experiment in cardamine seeds.

Nazhish from mumbai, she tryed to help them for test control experiment. Seed germination using kanamycin different concentration solution.

What is Kanamycin🤔?
Kanamycine is an antibiotics to kill bacteria in side the plants cells.

Then why only Kanamycin.
Other antibiotics
•Penicillin
•Streptomycin
•Ampicillin
•Tetracycline

1.Why not Penicillin :thinking:? Penicillin is also an antibiotics!
Penicillin is using for high fever like pneumonia.
2.If penicillin use as a medicine for high fever,can use malaria disease also🤔?
Penicillin is only using for bacteria caused diseases. Malaria is caused by protozoa.
IMG-20201214-WA0067 1.What is the difference between Bacteria & Protozoa :thinking:?
Bacteria doesn’t have nucleus in side the cell, so that’s why it is Prokaryotes.
Protozoa have nucleus in side the cell. So that’s why it is a Eukaryotic.
IMG-20201214-WA0071
1.How will Kanamycin kill bacteria in side the plant cell :thinking:?
Kanamysin is an antibiotics which are effect Bacteria (70s ribosomes). And also Mitochondria, Chloroplast also contain 70s ribosomes, so kanamycin is also kill bacteria as well as effect Mitochondria & Chloroplast. So that’s why the picture is shows that higher concentration of kanamycin will effect plants body. Seedlings growth will decrease and also plant color, green to change yellow.
IMG-20201214-WA0066
Nazish used Non transformed seeds for this Experiment. If Nazish use Ranchi Seeds ( Transformed seeds ) what will happen :thinking:? Will she get same results? or any variation come in the result?

[14/12/2020 - December 2020 – White Board.]
•We can use Streptomycin in the place of Kanamycin. Because Both of them are effect Ribosomes of Bacteria.
• Penicillin are effect the plant cell wall. Its using Animals cell because animals Doesn’t have any cell wall.