S3E53 Cube chatShaala: Exploring new ways of Learning

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Timing:5.30 pm to 9.30 pm
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CUBE ChatShaala on 31st December 2020
21 CUBists from 21 Centres had joined the webinar

Nellore: Tejasree

Jorhat: Ishita Sonowal

Patna: Kshipra

AND College: Aastha Ahuja
Dyal Singh College: Dr P. Chitralekha

Faridabad: Rahul Kushwaha

Sucheta Naik

Ranchi: Manjuel Jojo
Morabadi, Ranchi: Rechel Tirkey

Kozhikode: Arunima
Thriprayar, Thrissur: Aswathy Suresh
The Cochin College, Ernakulam: Essabell Philomina
Muvattupuzha: Fathima Ashraf
Thrissur: Rayis
Thanniam, Thrissur: Sidhy PP

South Bombay: Arunan MC
Nerul: Drishtant M Kawale
Powai: Lydia Mathew
Dahanu, Palghar: Sachin Pradhan

Moradabad: Kiran Yadav

Kolkata: Batul Pipewala

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Quoted by Dr. Arunan when @⁨Aastha Ahuja⁩ was making a schematic diagram of her plan of work…

Whiteboard screenshot ( 31/12/2020 ) CUBE CHATSHAALA

Bottom left side drawing shows the dry experiment what @⁨Aastha Ahuja⁩ has done today along with @⁨Rahul Kushwaha⁩ @⁨Arunan HBSCE⁩ and others…

Objective: to study the life span of fruit fly…
The bottom bottle which is marked with A actually the bottle in which one gravid female fly is transferred from the trap( shown in the top right side with banana pieces )

The Gravid femal fly will lay eggs in A bottle and than mother fly will be transferred in B bottle in suitable time till pupae will immerge out in bottle A

Again in B bottle mother fly will lay eggs, again the mother fly will be transferred in bottle C

Let’s come back to A bottle again to make things easy…
A bottle fly is already transferred and now A bottle has eggs , and on 10th day first generation will come out in A bottle and will called it as A '(now we know the Date of birth if this generation flies so we can go with our objective) and than again first generation will be transferred in A ‘’ bottle and and when these flies get matured they will mate and lay eggs there and subsequently flies will be transferred in to another bottles…

This was the dry experiment done by @⁨Aastha Ahuja⁩ and others.

Please give response and @⁨Aastha Ahuja⁩ please makes the things more clear if i made it little tricky…
References shared during the discussion

  1. Aging Studies in Drosophila melanogasterhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664065/
    Aging Studies in Drosophila melanogaster
    Yaning Sun,1 Jason Yolitz,1,& Cecilia Wang,1,& Edward Spangler,1 Ming Zhan,2 and Sige Zou1,*

  2. " Lifespan measurement" is the basic method used to determine the effects of genetic and
    nongenetic factors involved in aging (5). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664065/

Quoted by Aastha Ahuja

Yes ! So in our dry experiment our goal was to find out life span of fruit fly

So to know that we have to follow 3 steps :-

Trapping of flies

So to trap flies we will use tomato , banana , orange fruits as a bait to trap flies in a bottle of 500 ml. Then select one gravid female fly from that trap bottle to create single line cultures

Single line cultures ( replicates :- A, B, C bottle )

So the selected gravid fly is made to lay eggs in A bottle for 24 hours ( let’s call it day 1:- 1jan 2021 )

Then next day the gravid female fly is transferred to bottle B ( day - 2 :- 2Jan 2021 ) where it will lay the eggs for 24 hours in bottle B

Next day ( day :- 3 , 3 Jan 2021) we will transfer the gravid female fly in bottle C so that it can lay eggs in bottle C too … So we will now leave our gravid female fly in bottle C because our goal was to get 3 single line cultures from a single mother ( to create replicates for our experiment )

*What happens in bottle A in upcoming 20 days

On 1 Jan 2021 eggs were layed in bottle A which are expected to convert into flies in 10 days since the temperature in faridabad is low ( 23°C max , 6°C min) a longer time for flies to complete their life cycle is expected in low temperature area . so on 10 Jan flies are expected to emerge from pupa .

Now next problem :- we wanted to know the birth and death date of these flies , so we cannot allow them to get mixed with next generation flies which are expected to emerge in next 12 days …

So for that we expect that flies will attain their sexual maturity in 2 days post their birth and they will start to lay eggs too , so on 12 Jan 2021 they are expected to lay eggs which will convert into pupa in another 6 days
So as soon as we see the first pupa in bottle A ( also called A’ ) we will transfer flies from A’ to A"

20 Jan 2021- A’ bottle - F2 generation flies

20 Jan 2021- A" bottle flies whose birth date is 10 Jan 2021

This process of separation of flies with birth date 10 Jan 2021 from F2 generation , will take place every week until their death

Similar things are expected to happen in bottle B but one day late , and bottle C 2days post to bottle A

This was the dry experiment which we all did yesterday

I hope that I didnt complicate things more :sweat_smile: