301 CUBE CHATSHAALA
[19 JANUARY 2020]
Discussed about Fruit flies (one of model system ) @Raj came with an interesting research question fruit flies - behavioral study. His plan will be start observing normal behavior of fruit flies then after find abnormal behavior of flies from native place. To connect this study alzheimer’s disease in fruit flies.
@Saida & @Raj developed a methodology that how to find abnormal behavior of fruit flies in nature. A bowl take half of banana after flies where entered close the bait and observing their behavior.
Fruit Flies - Normal Behavior
@Raj is interest in study the behavior of Feeding & Defecations of fruit flies. So he want a better setup for study at his home lab. @Subsree also want to study in fruit flies.
How can start a culture of fruit flies at home lab🤔.?
• Take a tomato (tomatoes are good fruit for attracting fruit flies) , Cut two pieces.
•Put this tomato in a white paper, Kept near kitchen garbage bin.
•Observe flies are coming in the tomato pieces.(Night we can see very few number of flies, because flies are going to sleep.)
•Afternoon time (middle of day) we can see more flies.
How can we trapping these flies🤔.?
•Take an empty plastic bottle (narrow mouth bottle (~100ml,~250ml bottles). Make sure its transparent bottles.
•Keep bottle inverted (Because flies are moving upwards direction) - this way we can trap flies easily.
•Make sure gravid flies (mother flies) which are laying eggs. Looking their abdomen round, creamy colored, pointed abdomen, little bit bigger than males.
How can we study feeding habitat of fruit flies ?
When we trapped flies we can see flies are eating any time when they are Hungary.
->Considered 20 flies we trapped a culture bottles with media.we can see
• 8 flies (Female flies) are sitting over the media.
• 12 flies (considered as male flies) are can see the top of the bottles.
What they are doing in media ?
•Most females flies (8flies) are we can see over the media because of feeding, egg laying.
•Male flies always in top of the cotton plugs.
• Compared to female flies & male flies. Male flies are more active.
This is from normal observation.
This is an interesting research question,how can we studies it scientifically.How can we test it ?
How can we culture this trapped Fruit fly🤔.?
•After trapped we want a suitable media for culturing these fruit flies.
• TRSV Home media. (Tomato,Rava, Sugar - Vinegar media) culturing fruit flies at home lab.
TRSV - Media
1. One Egg sized Tomato
2. Rava - 2 Table spoon
3. Sugar - 1Table spoon
4. Water - 150mL (measure by sanitizer bottles)
5. Vinegar (Antifungal agent) - 15mL (measure by medicine cap)
•Take a pan put low flame - add 150 mL water, 2 table spoon rava, sugar, smashed tomatoes (using spoon) mixed the media very well till 10minutes.
•after 10 minutes off the flame take out pan from their, put 5 minutes for cool it down.
•after 5 minutes we can add 15 ml vinegar in to media, mixed it well.
•It look like a Dosha batter.we can easily por in to clean dry bottles.
•Kept for whole one day for solidification. Next day we can transfer flies in to that bottles.