S3E78 Cube chatShaala: Exploring new ways of Learning

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Timing:5.30 pm to 9.30 pm

Webinar will be recorded and recording link will be post in the same thread after the webinar.

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307th day of CUBE ChatShaala: 25th January 2021
19 CUBists from 17 Centres had joined the webinar
Map

DELHI
Garima Kalakoti
Dyal Singh College: Dr P. Chitralekha

KARNATAKA
Bangalore: Shama Sayyed

KERALA
The Cochin College, Ernakulam: Aishwarya Elizabeth, Manjula S Bhat
Kozhikode: Arunima
Thriprayar, Thrissur: Aswathy Suresh
Kandassankadavu: Lakshmy PJ
SN College, Nattika: Seethalakshmi CR
Palakkad: Shrudhiga, Sukanya P
Thanniam, Thrissur: Sidhy PP

MAHARASHTRA
Nerul: Drishtant M Kawale
Worli, Mumbai: Isha Pawle
Powai: Lydia Mathew
Mahad, Raigad: Manasi Prasad
Dahanu, Palghar: Sachin Pradhan

UTTAR PRADESH
Moradabad: Kiran Yadav

WEST BENGAL
Kolkata: Batul Pipewala

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25th January 2021
Effect of temperature on Life cycle of Fruit flies or on activities of Fruit flies?.

As many times we had discussed that Raj has been trying to catch fruit flies at place where temperature is min.13’ and max 24’ now a days…

But couldn’t find any fruit flies on the other hand two fruit flies got trapped in trap today where the temperature is max.20’ and min 6’C…

Is there any effect of Low temperature on fruit flies?..

So this was related with Rotte ning of fruit at low temperature and diffusion of Molecules in to the surrounding…
Fermentation get slows at low temperature that’s why Fruits will take long to get rotten…
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Can Aswathy also observe the effect of temperature on the life cycle of Fruit flies at her CUBE HOME LAB…

A design was made to varify the statement…

One bottle will be kept as control where a female gravid fly will allowed to lays eggs and after it lays .the fly will be removed from the bottle and bottle will be kept outside…

Where as in Test bottle, the bottle will be kept in side the fridge after the fly lays eggs…

Questions:would larvae survive at low temperature in fridge?.

Would it take more days to complete life cycle??.

Would it be possible to find cold tolerance fruit flies?.

These are the sort of questions that were being asked…
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[11:18 PM, 1/25/2021] KIRAN CUBE: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4811728/
Natural variability in Drosophila larval and pupal NaCl tolerance

The regulation of NaCl is essential for the maintenance of cellular tonicity and functionality, and excessive salt exposure has many adverse effects. The fruit fly, Drosophila melanogaster, is a good osmoregulator and some strains can survive on media with very low or high NaCl content. Previous analyses of mutant alleles have implicated various stress signaling cascades in NaCl sensitivity or tolerance; however, the genes influencing natural variability of NaCl tolerance remain for the most part unknown. Here, we use two approaches to investigate natural variation in D. melanogaster NaCl tolerance…
Effect of concentration of salt on life cycle of fruit flies?. Similarly can we find out the effect of salt…

We got an update from Aswathy’s fruit flies single line Cultures…
Aswathy’s further plan is to perform olfactory assay at home…

We had discussed about olfactory assay.

Questions: what’s the objective of performing olfactory Assay?.

  1. How do we calliberate olfactometer?.

  2. Why Larvae should move towards Banana Paste when they have choice to eat Rava?.

  3. Does Rava has any kind of smell?.

  4. How to extract 2nd Instar Larvae from TRSV medium. Bottle?.

  5. Why only 2nd Instar Larvae?why not other staged larvae or adult Fruit fly?.
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    How to extract 2nd Instar Larvae from TRSV MEDIUM BOTTLE?.
    Isha had joined the session and discussed the method that they were used to do in CUBE LAB…

why there’s need to transfer Larvae in Ringer’s solution?.
And as per Wikipedia Ringer’s solution contains sets of salt to maintain isotonic condition…
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We got an update from Isha’s fruit flies Cultures.

Isha has started making single line Cultures from one Gravid female fruit fly and Today 25-25 flies are transferred in another two TRSV bottles in order to maintain the Culture…

Celebration of Goof up.
The first bottle in which one single gravid fly was transferred is getting liquify as tomato puree was used while making TRSV MEDIUM.