S3E90 Cube Chatshaala: Exploring new ways of Learning

Meeting URL

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Timing:5.30 pm to 9.30 pm

As we do it daily, CUBE chatShaala will be starting at 5.30 pm and as a continuation of it, from 8.30 pm, there will be NAIL REGENERATION WORKSHOP today in CUBE chatShaala.

Looking forward to your participation.
Thanks

IMG_20210206_222655
06th February 2021
Picture presentation: How well we are able to observe things?.

Same Another observation made by Aswathy from one of the bait Culture of fruit flies is that Red color is seen at the posterior end of Larvae…

Discussion revolved around why this red color is seen in these Larvae?.

Some presumption made by the Cubists which are as follows

  1. Red color is seen because larvae are feeding on tomato and tomato is red in color, hence red color is seen in the mid gut.

  2. These could be of different species

  3. Comparison was made with Moina as moinas turn red when undergone low oxygen level condition, is color change can also be seen in larva of fruit flies?.
    https://febs.onlinelibrary.wiley.com/doi/full/10.1111/j.1742-4658.2008.06642.x link for hypoxia in larvae

  4. may be a varient

  5. Another presumption made that due to to ph of tomato.
    https://www.researchgate.net/publication/303774643_Molecular_mechanism_and_functional_significance_of_acid_generation_in_the_Drosophila_midgut
    How do we varify all these presumption?..
    IMG_20210206_222441
    https://www.researchgate.net/publication/303774643_Molecular_mechanism_and_functional_significance_of_acid_generation_in_the_Drosophila_midgut. Midgut pH assays. pH was measured in the larval midgut using ymol blue, m-Cresol purple, Cresol red, Bromocreosol purple, Bromothymol blue, Phenol Red and Congo red indicator dyes (all purchased from Sigma- Aldrich). Indicators were added to melted Drosophila diet (0.1% w/v), immediately mixed, and allowed to cool to room temperature. Larvae of the appropriate genotype were added, and aer 2 hours the midgut excised in Schneider’s insect medium (Invitrogen). Micrographs were taken immediately using a Sony CyberShot NEX-C3 mounted on a Leica stereo microscope, as pH remains stable for only a few minutes aer dissection. Images were processed using Adobe Photoshop CS5.1. Where noted, the diet was also supplemented with 1 mM Omeprazole (Sigma-Alrdich) or 100 μ M acetazolamide (Sigma-

Screenshot_2021-02-06-23-04-41-03
Bait(where one slice of tomato) was kept on 17th January 2021 in order to trap fruit flies from Native(Moradabad UP).

One Gravid fruit fly got trapped on 25th January 2021(on 9th Day).

First Larva(4mm) was seen on 03rd February 2021

First pupa was seen on 06th February 2021.
Objective: to study life cycle of fruit fly
IMG_20210206_222340
Approx 5mm size Larva seen on the top of bottle today (06th February 2021)
IMG-20210206-WA0089
Nail Regeneration Vs. Nail Growth
How does Nail grow?
How do they Regenerate?.

1 Like

319th day of CUBE ChatShaala: 6th February 2021
25 CUBists from 23 Centres had joined the webinar
Map

ANDHRA PRADESH
Nellore: R Srivalli, Tejasree B, Ujwalaraj

ASSAM
Sonari: Susanta Tanti

DELHI
AND College: Aastha Ahuja
Dyal Singh College: Dr P. Chitralekha

JHARKHAND
Morabadi, Ranchi: Rechel Tirkey

KERALA
Thriprayar, Thrissur: Aswathy Suresh
Kandassankadavu: Lakshmy PJ
SN College, Nattika: Seethalakshmi CR
Thanniam, Thrissur: Sidhy PP
Kozhikode: Theertha

MADHYA PRADESH
Indore: Farhan Ashraf

MAHARASHTRA
South Bombay: Arunan MC
Nerul: Drishtant M Kawale
HBCSE, Mumbai: DP
Thane: Hitesh Pareek
Worli, Mumbai: Isha Pawle
Powai: Lydia Mathew
Mulund, Mumbai: Priti Kanade
Dahanu, Palghar: Sachin Pradhan

UTTAR PRADESH
Kanpur: Hina Mudgal
Moradabad: Kiran Yadav

WEST BENGAL
Kolkata: Batul Pipewala

J. Sujitha