S4E126 CUBE chatShaala: "Experimental design to investigate- Do fruit flies sleep?"

We had an interesting discussion on sleep, where the context was fruit fly (Drosophila). what is sleep in fruit flies? How do we define sleep? Do microbes or rotifers sleep? Cubists also made one design to verify the hypothesis: fruit flies do sleep.

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References

http://brain.harvard.edu/hbi_news/why-severe-sleep-deprivation-can-be-lethal/#:~:text=Contrary%20to%20the%20gut-targeted,ROS%20accumulation%20in%20the%20gut. Severe sleep deprivation kills flies

In fruit flies, sleep can be conveniently estimated by measuring the locomotor activity of the flies using techniques and instruments adapted from the field of circadian behavior.

To facilitate the genetic study of sleep, we documented that rest behavior in Drosophila

melanogaster is a sleep-like state. The animals choose a preferred location, become

immobile for periods of up to 157 min at a particular time in the circadian day, and are

relatively unresponsive to sensory stimuli.https://www.sciencedirect.com/science/article/pii/S0896627300808776

Sleep was defined as any period ≥5 min without an activity count, as previously determined (Hendricks et al. 2000; Shaw et al. 2000; Huber et al. 2004; Ho and Sehgal 2005). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2323820/#bib25

In order to be considered sleep-like, an inactive state should have the following features (6, 16): (1) consolidated circadian periods of immobility, (2) a species-specific posture and/or resting place, (3) an increased arousal threshold (although the state can be reversed by intense stimulation), and (4) a homeostatic regulatory mechanism.Joan CHendricks1*Stefanie MFinn1Karen APanckeri1JessicaChavkin1Julie AWilliams1AmitaSehgal1Allan IPack1

Sleep is also present in invertebrates, and an extensive analysis in Drosophila melanogaster has shown that sleep in fruit flies shows most of the fundamental features that characterize sleep in mammals.Sleep and wakefulness in Drosophila melanogaster https://pubmed.ncbi.nlm.nih.gov/18591491/

As described, sleep in Drosophila and other insects is largely measured through behavioural metrics, notably immobility https://www.frontiersin.org/articles/10.3389/fphys.2019.01167/full

Nematodes

Vinegar is acidic. Vinegar’s pH level varies based upon the type of vinegar it is. White distilled vinegar, the kind best suited for household cleaning, typically has a pH of around 2.5.https://www.healthline.com/health/ph-of-vinegar#_noHeaderPrefixedContent

The Microworms feed on the yeast and bacteria produced from the oatmeal. After 3 to 7 days, the surface of the media will appear to shimmer with the movement of the Microworms produced and they will start climbing up the sides of the container.http://fisheries.tamu.edu/files/2013/09/Culture-O-Microworms-Panagrellus-sp.-As-An-Alternative-to-Brine-Shrimp-for-Larval-Fish-Forage.pdf

We determined that SCFA-attractive responses depend on two larval exclusive chemoreceptors, Or30a and Or94b. Of those SCFA, propionic acid improves larval survival in suboptimal rearing conditions and supports growth. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5660182/

Propionic acid is a bactericidal and functions as preservative and increases the shelf life of food. https://www.intechopen.com/online-first/75974

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495th Day of CUBE Chatshaala: 27th July
29 CUBists from 23 Centres had joined the webinar

Assam
Jorhat : Ishita Sonowal

Delhi
ANDC : Aastha Ahuja
New Delhi : Raj Yashwantrao Patil
DSC, Delhi : Dr P. Chitralekha

Kerala
Kozhikode : Arunima
Thriprayar : Aswathy Suresh
Palakkad : Dr. Nisy Prasad
Kandassankadavu : Lakshmy PJ
Alapad, Thrissur : Vishnupriya CJ

Maharashtra
Kalyan : Aditya Joshi
Ghatkopar, Mumbai : Anjanikumar
Thane : Anshu Kadam
Breach Candy, South Mumbai : Arunan MC

Wardha : Dilip Raghavan
Worli, Mumbai : Isha Pawle

Wardha : Janhvi Deshmukh
NES Ratnam College, Mumbai : Kareena Dhuri
Powai : Lydia Mathew
Bandra, Mumbai : Saida Sayyed
Panvel : Shraddha Sonavane
NES Ratnam College, Mumbai : Sunita Chahar
Prabhadevi, Mumbai : Yash Sheregare

Uttar Pradesh
Kanpur : Hina Mudgal
Moradabad : Kiran Yadav

Uttarakhand
Sakshi

West Bengal
Kolkata : Batul Pipewala
Kolkata : Sukriti Mai
Diya Ekka

Anurag Kumar Pandey

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