Summary by @saswathy679
SUMMARY - CUBE Chatshaala
7th August 2021
NEMATODES
Yesterday started the discussion with BREAKING NEWS FROM CHANDINI KOLKATA - WEST BENGAL
@Batul from Kolkata successfully isolating and culturing nematodes from her home lab - Chandini Kolkata West Bengal.
Research Q : Find native C.elegant from native soils using Raw Potato, Moist Soil, Milk.
WHY NEMATODES ?
C.elegants are coming under Nematodes. Want to find C.elegants some other nematodes looking morphology features.
IMPORTANCE OF C.ELEGANS!
C. elegans Nobel prizes
The Nobel Prize in
Physiology or Medicine 2002
The Nobel Prize in Physiology or Medicine 2006
The Nobel Prize in Chemistry 2008
•Where Can We Find This C.Elegants? C.Elegants are Found Only in Soil?
Yesterday Batul ma’am, shared one article says is that C.elegants also present in Rotten fruits as well.
Reference A phylogeny and molecular barcodes for Caenorhabditis, with numerous new species from rotting fruits | BMC Ecology and Evolution | Full Text
Nematodes Picture from @Batul Home lab Chandini Kolkata West Bengal.
HOME LABS FROM DIFFERENT PARTS OF COUNTRY. : Studies particular model systems based on their research questions (Fruit Flies, Nematodes, Phyllanthus, Ants etc.)
Isolation on an agar culture plate.The easiest way to recover nematodes from soil is simply to place the substrate around the bacterial lawn of a standard C. elegans culture dish (see Maintenance of C. elegans). Bacteria-feeding nematodes such as C. elegans are attracted and crawl out of the sample towards the bacterial lawn (Figure 1). For fast-moving species like C. elegans, this occurs within minutes to hours.
http://www.wormbook.org/chapters/www_nematodeisolation/nematodeisolation.html
also discussed about DIFFERENT TYPES OF NEMATODES. Parasitic Nematodes example Meloidogyne incognita or Root-knot nematode
Meloidogyne incognita is probably the most economically important plant-parasitic nematode species among the tropical and subtropical regions. This nematode is extremely polyphagous, attacking both monocotyledons and dicotyledons. It is estimated that more than 3,000 plant species can be affected.[6][7][8]Meloidogyne incognita - Wikipedia
Root - Knot Nematodes are 15-16 Macron meters.
C.elegants are 1-2 Millimeters.
What is the Difference between Microns & Millimeter.
1000 MIcro meters = 1 Millimeters
Examples of micron size : RBC, Bacteria etc. (also growth of nail is macron meter)