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We maintained cup cultures for earthworms by using cellulose as their source of food. Our objective is to check the effect Valproic acid which is an HDAC inhibitor on the regeneration in earthworms.
We made 3 groups each containing 5 earthworms. Group A contains the control in which the earthworms are amputated at 40th segment and both the parts are kept seperately in different cups and is named as CE1A & CE1P meaning control earthworm 1 anterior part after amputation.
Similarly group B contains the worms amputated at 40th segment but treated with 1mM concentration of VPA at the site of amputation and named EE1A(1mM) & EE1P(1mM) and so on.
Similarly group C contains the worms amputated at 40th segment but treated with 2mM concentration of VPA at the site of amputation and named EE1A(2mM) & EE1P(2mM) and so on