The Story of 3 AM flies in my HomeLab, Nallalam, Kozhikode

On 7th June ChatShaala, @Sailekshmi shared how she conducted Food Preference Test in Fruitflies at her Homelab. I got excited when she said that she kept the bait at 10 pm, that is at the night time!!
Then there came a question- What if we keep a freshcut tomato slice at night time? Will the fruitflies come? And after how many hours can we expect fruitflies??? If we ever spot flies at midnight, can they be sleep mutants!!? So many questions to be answered! So getting inspired from the ChatShaala discussion, I set up a bait at 10 PM the same night, June 7. I arranged a slice of freshly cut tomato on a white plate and kept in on my kitchen slab.
I was so much excited!!! Will any flies visit my tomato slice at night time?:sleeping:



Observations recorded during the night and early morning. No flies were observed on the set up.
Nallalam, Kozhikode
What might the fruitflies do at night time? @⁨~M S Sailekshmi⁩ @⁨Theertha Cube⁩ @⁨Cube Seethalakshmi⁩

Next day - June 8, 2025
3 flies are observed on the tomato slice at 6am!!!
Nallalam, Kozhikode

The number became 4 at 8 am in the morning.

25 flies at 9.50am in the morning on 8/06/25!!!
This is a huge number when I compare with the observations of my previous food preference studies
@⁨~M S Sailekshmi⁩

27 flies at 11 am on 8/06/25
Nallalam, Kozhikode
@⁨~M S Sailekshmi⁩

5 flies at 11.21am on the tomato slice!!
@⁨~M S Sailekshmi⁩
At what time do you expect to see more flies? And share your observations too. Let’s compare @⁨~M S Sailekshmi⁩

43 fruit flies at 1.30pm on a tomato slice. Nallalam, Kozhikode. Photo: Ayana @⁨~M S Sailekshmi⁩


44 fruit flies at 2.30pm on tomato a slice kept in my kitchen @⁨~M S Sailekshmi⁩

29 flies at 6pm

16flies at 7pm

3 fruitflies observed on 9/06/25 at 3 am.

9/06/25at 3am are they sleep mutant flies?
@⁨Batul Ma’am⁩ @⁨~M S Sailekshmi⁩ @⁨HBCSE Arunan Sir⁩ @⁨Theertha Cube⁩ @⁨Cube Seethalakshmi⁩

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Now trapping the 3AM flies!!!
On close observation it was known that the two were gravid fruitflies. So what if I capture the pregnant flies in two different clean plastic bottles? Will the progenies of these mother flies be also active at 3 AM??


3AM Fly -A inside the bottle A

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