September 5th 2019
Long term objective :- to study ventral nerve regeneration in earthworms ( e.fetida)
Short term objective:- to perform rapid escape response assay (RER)
Immediate objective:- to maintain cup cultures
Today I’m going to perform RER assay with 15 earthwoms.
In this assay We’ll take 30 testubes with 2 test tubes for each eartworm.
In one test tube we’ll take distilled water
In other we’ll have 200mM NaCl solution
First we add the eathworm in distilled water and start timer of two minutes after 2min we transfer it to NaCl and put timer of two minutes.
The eartworm will not come out of disstilled water as it contains no ions in two minutes
And in NaCl it will come in two minutes .