We started discussion with Nandini from Nellore, as she is working on zebrafish Nandini uses Artemia to feed zebrafish, here artemia grows and live in salt water. Where as Zebrafish is fresh water organism So before feeding we have to take the artemia cyst and add 1lit. tap water and a spoon of salt in it After 24hrs larve of artemia would be seen so we can wash and feed it to zebrafish But rather than doing this, can’t we use other fresh water organism like moina Or daphnia? What would zebrafish will be eating in natural fresh water body like river? How do we get moina in Nellore? Then sakshi explained how she cultures moina by using 250ml plastic bottles With 200ml of DC water and 1 drop of milk everyday with help of dropper When we add milk, bacteria in the water feed on the milk and multiple, then moina feeds on those bacteria Where if we take 5 moina today then after 3 days we will get 15 moina in the bottle
What would happen if we add more milk in the culture? As amount if milk increase⬆, Bacteria will also increase⬆ and as Bacteria uses more oxygen, amount of oxygen in watter will decrease⬇️ So here Biological Oxygen Demand (BOD) is more as oxygen is less so to get as much as oxygen it can, moina will produce more hemoglobin Therefore moina will turn red in presence of hemoglobin due to more milk, bacteria and less oxygen So here CUBist study about Epigenetics to know if this red color of moina will pass on from mother to offspring So is it possible?@Theertha @Ichha @sakshiconsultant2002 @Himanshu @jaikishan @Abhi0703 @Misbah and others
Reference shared during chatshaala discussion 
For example, having a mutation in the BRCA1 gene that prevents it from working properly makes you more likely to get breast and other cancers.
https://www.google.com/url?sa=t&source=web&rct=j&url=https://www.cdc.gov/genomics/disease/epigenetics.htm%23:~:text%3DCertain%2520mutations%2520make%2520you%2520more,get%2520breast%2520and%2520other%2520cancers.&ved=2ahUKEwjeirWZprP8AhXvgWMGHYSaCt4QFnoECGsQBQ&usg=AOvVaw3C9XpgKAS5fY7E28-NQnqU