Archives of CUBE Context 2 Curriculum what's app group discussion

KiranKalakotiR · August 29, 2021, 7:58am

23rd August 2021, 9.30pm to 24th August 2021, 9.30pm
Mango tree not started flowering!

Flowering not started
No sprouting
No Fruiting Yet.

Date : 23.08.2021,
Time : 6:35 PM

Latitude: 19.0237° N, 73.0404° E

Temperature :
Maximum : 25°C
Minimum: 31°C

Location: CBD Belapur,(Mango Garden) Navi Mumbai, Maharashtra
CUBE Homelab CBD Belapur
Data Collector: Swayam Gaikwad
Mango tree 2 not started flowering!

Flowering not started
No sprouting
No Fruiting Yet.

Date : 23.08.2021,
Time : 6:35 PM

Latitude: 19.0237° N, 73.0404° E

Temperature :
Maximum : 25°C
Minimum: 31°C

Location: CBD Belapur(Mango garden) Navi Mumbai, Maharashtra
CUBE Homelab CBD Belapur
Data Collector: Swayam Gaikwad

VIDEO SHARED BY DRISHTANT AT 12.20AM
Fungus in its own niche captured via mobile microscopy
Growing on an empty stalk of a custard apple.
Are these conidia? What do we call the white filament-like structures?

Taken via a convex lens obtained from laser light.
No digital zooming was done.

Magnification: Unknown
Place: Nerul, Navi Mumbai
Date: 23rd August 2021
Video: Drishtant MK
White stalk is Sporangium and tip bulbous round structure is Sporangiophore inside of which is spores r present
Great
What do you mean by non - moving larvae like structure? Is it pupae?

This is beautiful. It is tough to imagine that ugly looking fungus can appear so good when magnified. What are the typical features by which we distinguish fungus?

Mushrooms also have a similar structure. Do their heads also have spores? Or am I completely off?
[8:59 AM, 8/24/2021] Arunan HBSCE: Can we get a longer shot where this set up is placed? @Batul Pipewala Kolkata
[9:07 AM, 8/24/2021] Arunan HBSCE: " This nematode is particularly abundant in microbe-rich environments, especially rotting plant matter such as decomposing fruits and stems. In this environment, it is part of a complex interaction network, which is particularly shaped by a species-rich microbial community. These microbes can be food, part of a beneficial gut microbiome, parasites and pathogens, and possibly competitors. C. elegans is additionally confronted with predators; it interacts with vector organisms that facilitate dispersal to new habitats, and also with competitors for similar food environments"
" Full appreciation of this nematode’s biology warrants further exploration of its natural environment"
A great introduction @Batul Pipewala Kolkata to look for C elegans in Indian/ Srilankan soil @Batul Pipewala Kolkata @Anshu Kadam (Nematodes) @Ishita Assam
[9:11 AM, 8/24/2021] Arunan HBSCE: Please give reference link too @Ankit Yadav Xms Work Shop Cube Patkar College
Please give a description @Dr. G C Baskey Cube Ranchi
Please send the photos, Videos and data for the CHAI and WHY?
Fruitfly:- @Aswathy Suresh CUBE @Isha Pawle @Yash Sheregare CUBE @Ishita Assam
Cardamine:- @Arunima SN Nattika Cardamine @Shraddha Sonavane CUBE @Rechel Turkey Cube Ranchi Moina @Nazish Cube Drosophila @Lakshmy PJ CUBE
Nematodes:- @Arunima SN Nattika Cardamine @Anshu Kadam (Nematodes) @Ishita Assam
Phyllanthus:- @Man Masih Beck RANCHI CUBE @Lakshmy PJ CUBE @Shraddha Sonavane CUBE @p.chitralekha
Yeast:- @Seethalakshmy CUBE @Aditiya Joshi CUBE
[9:22 AM, 8/24/2021] Arunan HBSCE: Why on 7th day?@Man Masih Beck RANCHI CUBE What’s the reasoning? @@Man Masih Beck RANCHI CUBE @Aswathy Suresh CUBE
[9:24 AM, 8/24/2021] Arunan HBSCE: Please make your argument more clearly using some reference too @Shivam CUBE NEMATODE
This looks fine! @Lakshmy PJ CUBE
[9:27 AM, 8/24/2021] Arunan HBSCE: Time and date please! @Lakshmy PJ CUBE
[9:29 AM, 8/24/2021] Arunan HBSCE: Good to see you back @Nazish Cube Drosophila

Colonies on boiled potato slices

Few colonies on boiled potato slices

Boiled potato slices in a plastic box

Dipping cotton buds for spreading fermented coconut water on boiled potato slices. Aditya Joshi, Kalyan west

Bottles kept for fermenting coconut water. Aditya Joshi. Kala Talav, Kalyan west.

Phyllanthus growing in pots n plastic box. Aditya Joshi. Kala Talav, Kalyan west.

These are few photos of of toddy experiment setup n colonies and PhyllanthusAfter how many days? Please post a summary starting with Objectives @Aditiya Joshi CUBE and interpretation.
Objectives and some essential summary including further plan of work, please @Aditiya Joshi CUBE
Objective : To study the effect of Continous light on sleep -wake cycle of Phyllanthus

Plants kept in grill,store room and under the table
Can we please get previous national meet videos

Fruit Flies are present on Tomato kept fruit basket observed in kitchen.

Picture Credit : Shaharubanu CUBE SN Nattika.
Date : 24.08.2021, Time : 11:46am

Fruit flies observed in kitchen garbage bin lid area.

Picture Credit : Shaharubanu CUBE SN Nattika
Date : 24.08.2021, Time : 11:51am

Fruit Fly - On Pineapple Slices

Picture Credit : Shaharubanu CUBE SN Nattika
Date : 24.08.2021, Time : 11:57am

Close Up Picture Of Fruit Fly.
Picture Taken : Shaharubanu CUBE SN Nattika.
Date : 24.08.2021, Time : 11:46am

While walking through my local park I saw a slug (although it was alone) on the pavement. Does this mean a future revival can take place for the Cube Ghatkopar Snail homelab as snails and slugs are similar to one another and belong to the same class gastropoda:flushed:

VIDEO SHARED BY RECHEL AT 12.05PM
The floral dip method by Ranchi cubists @⁨Manisha Di Botany⁩ @⁨Dr Shalini Lal⁩ before lockdown. This was also presented in previous CUBE national meet.
Slides used to make the video for cardamine floral dip method
Uploading: DR2.jpeg…

Width of leaves of different species of Phyllanthus plants. location-Panvel.Data collected by-Shraddha

Cardamine plants from home lab, Panvel. 3 simple leaves & 1st compound leaf with partially developed & differentiated leaflet ,2nd compound leaf with completely differentiated leaflet
Cardamine plants grown without soil (on moss ) showing Cardamine & moss interactions. Location- Panvel data collected by-Shraddha

Phyllanthus urinaria plants kept in continuous light experiment.

Phyllanthus plants kept in continuous dark experiment

Phyllanthus urinaria characteristics features.

Please send photos, video, presentation of previous national meet in the group as soon as possible!
@Nazish Cube Drosophila @Arunima SN Nattika Cardamine @Yash Sheregare CUBE @Drishtant Cube Moina @Anshu Kadam (Nematodes) and others!!

How to compare & identify Phyllanthus plants from other leguminous plantsSeeds of Phyllanthus urinaria plants

Home lab set up for Continuous light and dark experiment in Phyllanthus urinaria plants.

A picture from earlier video of National Meet 2020. The 6 Nobel Prize Winners and the 6 Time Noble Prize winning Model system - Fruit fly!

Phyllanthus urinaria plants kept in normal sunlight as control for continuous light and dark experiment

How to compare & identify Phyllanthus plants from other leguminous plants
Phyllanthus urinaria.
Data collected by: Theertha
Date:24/8/2021
Place: Cherukkad, Kozhikode.
CUBE SN Nattika
Phyllanthus amarus.Data collected by: Theertha.
Place: Cherukkad, Kozhikode
Date:24/08/2021
CUBE SN Nattika
Phyllanthus debilis.Data collected by: Theertha.
Place: Cherukkad, Kozhikode
Date:24/08/2021
CUBE SN Nattika

Fruit fly culture bottles with TRSV medium. Date: 01/09/2020. Location: Thanniam , Thrissur, Kerala. Sidhy.P.P , CUBE S N College, Nattika.

Cardamine plant .close relative of arabidopsis thaliana.

Cardamine plant development of floral buds to seeds

Preparation of TRSV medium for fruit fly culture. CUBE Home lab Thanniam, Thrissur , Kerala. By Sidhy.P.P , CUBE S N College, Nattika.Chai and why? Nematodes – Google Drive

Nematodes @Chai and why@@Saida Sayyed @Abhijeet Singh
Preparation of 150 ml of TRS-V Medium

Ingredients
Water:cup_with_straw:( 150 ml)
Egg Size Tomato (~50 gm)
2 table spoon Rava:rice:(20 gm)
1table spoon Sugar (15 gm)
Vinegar (15ml)

Protocol
1.Taken a pan. Turn on the flame.
2. Add ~150ml water ,2 table spoon rava ,1 table spoon sugar.
3. Then add 1smashed tomato.
4.Mix continuously for 10 minutes at low flame .
5. Turn off the flame and leave it for cooling for 5mins.
6.Then add ~15ml Vinegar in medium and mix it well.
7. Pour in to clean and dry bottle and add cotton plug to it.
8. Leave it for solidification for 24hrs
9. After 24 hrs transfer flies to the media bottle.

Video Credit- Isha Pawle
While uploading the Google drive link in the group Please make sure that Google drive folder includes all the photos videos and all the stuff of the particular model organization of all the collaborators working on in cube on that!

the excess to the Google drive link should be to everyone
Mobile Microscopy:microscope:

To observe the fly using any microscope we first need to immobilise the flies.
Transfer some flies from a single line culture into a empty bottle.
Keep this bottle into refrigerator.
After 1 hours Remove the bottle and we can see flies getting immobilise.

Requirements For Mobile Microscopy
1- Torch🔦
2- Mobile camera📱
3- White Sheet Papar📄
4- Glass Sheet or a Transparent sheet Paper
5- Needle
6- Brush
And 7- Immobilised Flies

Setup for Mobile Microscopy
Starting from the bottom first place a TORCH
Above the torch place A WHITE SHEET PAPER
Then above it place a GLASS SHEET.
At last place our object i.e. the FLY
Take a MOBILE CAMERA and adjust the focus accordingly. We can zoom in or zoom out to get a clear picture of the fly.

Video credit - Isha Pawle

Seeds harvested from cardamine plant

Phyllanthus model system.
Identification
Plant : Phyllanthus tenellus.
Long stalked phyllanthus.
Erect annual herb.
Leaves:
Alternate,leaf hair absent,oblong in shape(subacute -leaf tip,acute to rounded - leaf base)
Fruit :
Capsule:Smooth.
On short stalked .
Flowers with 1.5-5 mm long stalks.
5 tepal.Stem-irregular round

Date:24/08/2021.
Place: Cherukkad,Kozhikode.
Data collected by: Theertha.
CUBE SN Nattika.
https://drive.google.comhttps://drive.google.com/file/d/14isoHZ6A0ZHiznkyaUh0-kqJaXhtxxGw/view?usp=drivesdk
Title: "Isolation of soil nematodes from soil sample on a raw potato surface in Home labs”

https://drive.google.com/file/d/1kCklrqGWlJ7ji9ont0iDgOZA6-5ozFGG/view?usp=drivesdk[6:53 PM, 8/24/2021] Sunita Chahar CUBE: Phyllanthus niruri: Benefits, uses, and risks
[6:57 PM, 8/24/2021] Sunita Chahar CUBE: The Genus Phyllanthus: An Ethnopharmacological, Phytochemical, and Pharmacological Review
[6:59 PM, 8/24/2021] Sunita Chahar CUBE: https://www.cabi.org/isc/datasheet/46049
[7:01 PM, 8/24/2021] Sunita Chahar CUBE: http://www.bioinformaticssikkim.gov.in/medicinal%20Plants/131.html

rapping Fruitflies for Studying the Activity Pattern of Fruitflies in July 2018 - Near the biogas plant kachada dubba n can with fermented rice water near by to attract fruitfly.

Photo: Sulekha Sudhakaran, CUBE Locality Lab, Paruthiara, a CUBE Node of the CUBE Hub, Vennicode ( lThiruvananthapuram Dist.).

CUBE Ranchi Re-Mans’ Home Lab
24-08-2021
Fruitfly Update

Breaking News
2 pupae observed on the 6th day of transfer

Immediate Objective: to start a single line fruitfly culture(RNSL-B) at ReMan’s Lab in TRSV Media

Short Term Objective: -To identify the trapped fruitflies
-To get native Drosophila melanogaster species

Media preparation- 18-08-2021
Transferred date- 19-08-2021

OBSERVATION
on the 6th day of transfer
•the transferred fly
•~16 larvae
•~2 pupae
•~1 fly

Expectation
-Earlier Expectation: New flies will emerge on 27th Aug, 2021(pupae developing on 26th Aug, 8th day of transfer)
-New Expectation: New flies will emerge on 25th Aug, 2021(pupae developing on 6th day of transfer) @Aswathy Suresh CUBE @Arunima SN Nattika Cardamine @Drishtant Cube Moina

KiranKalakotiR · August 29, 2021, 7:58am

24th August 2021, 9.30pm to 25th August 2021, 9.30pm

VIDEO SHARED BY SIDHY AT 11.35PM
SLTT-H
Single line culture of fruit flies.
Started on 16/08/2021, 11:40 AM
TRSV medium - 15/08/2021, 11:00 PM

Expectation:
Pupae on fourth or fifth day 20 , 21/08/2021
New flies will emerge on 8 th day ( 24/08/2021)

Observation on 24/08/2021
~ 30 larvae
~ 21 pupae
~12 flies

Location : Thanniam, Thrissur, Kerala
Sidhy.P.P, CUBE S N College , Nattika.
SLTT-I
Single line culture of fruit flies.
Started on 16/08/2021, 11:40 AM
TRSV medium - 15/08/2021, 11:00 PM

Expectation:
Pupae on fourth or fifth day 20 , 21/08/2021
New flies will emerge on 8 th day(24/08/2021)

Observations : Pupae on 6 th day.
24/08/2021- ~ 40 larvae ,10 pupae ,No new flies emerged.

Location : Thanniam, Thrissur, Kerala
Sidhy.P.P, CUBE S N College , Nattika.
SLTT-J
Less number of larvae and pupae on 8 th day

Single line culture of fruit flies.
Started on 16/08/2021, 11:40 AM
TRSV medium - 15/08/2021, 11:00 PM

Expectation:
Pupae on fourth or fifth day 20 , 21/08/2021
New flies will emerge on 8 th day(24/08/2021)

Observations : Pupae on 6 th day.
24/08/2021- ~ 10 larvae , 2 pupae ,No new flies emerged.

Location : Thanniam, Thrissur, Kerala
Sidhy.P.P, CUBE S N College , Nattika.
In this video i can see your voter mark
Is it still there?

By looking at the Pupae what do you predict?
When are you hoping to see flies coming out of pupae? @Sidhy Kerala Drosophila
Can you post some close up photos of pupae?
[8:55 AM, 8/25/2021] Arunan HBSCE: Why not celebrate by giving a BREAKING NEWS STORY with HEADLINES? @Sidhy Kerala Drosophila
[9:03 AM, 8/25/2021] Arunan HBSCE: Wonderful observation! A fine detective work like that of Sherlock Holmes! @Seethalakshmy CUBE @Sidhy Kerala Drosophila
Almost 120 day’s the Voter’s mark remains in this Nail!

Wow! @Sidhy Kerala Drosophila @Seethalakshmy CUBE

By looking the pupae , the expectation was that the flies will come out of the pupae today 25/08/2021. In SLTT- I and SLTT - J , one fly came out of the pupae today morning. These pupae were formed on 22/08/2021. There had 5 pupae formed on same day. From 2 of them flies came out. Hope that the remaining 3 flies also will come today.
From the pupae (in the photo) the flies will come out soon.
What would you write as a BREAKING NEWS HEADLINE? @Sidhy Kerala Drosophila

BREAKING NEWS 118day old nail mark !! Date of mark 29/4/21 date of pic 25/8/21 Gender F, Age53, No of days 118days, Growth of nail 12mm.Cube Homelab Kolkata BatulP
Not much difference in nail growth over 120days between 53yr old female and @Sidhy Kerala Drosophila female age 21
Pic of 120day old nail mark shows growth of 13mm@@Sidhy Kerala Drosophila
New generation on 9 th day in SLTT-I and SLTT- J. One day later than the expectation.

The expectation was pupae on 5 th day and flies on 8 th day. What happened is , pupae formed on 6 th day and flies emerged on 9 th day. The period from pupae to fly is same as the expectation. Flies emerged on third day after the formation of pupae.

SLTT- H , which started along with I and J , got new generation on 8 th day
New Generation of Fruitflies from Single Motherfly? @Sidhy Kerala Drosophila
Before the Pandemic Fly culture 20 Feb 2020 CUBE HBCSE Photo : CUBE Archives mcarunan

Phyllanthus closing leaves at 6:02 pm From CUBE Archives 2019 mcarunan Please see the next pic.Yes Sir. SLTT-H , SLTT-I and SLTT-J are newly started single line cultures of fruit flies. Transferred one gravid fly to each bottle on 16/08/2021.
Phyllanthus leaves open at 9:02 am From CUBE Archives 2019 mcarunan Please see the previous pic.

Whiteboard screenshots of Sleep wake cycle of fruit fly and opening and closing of Phyllanthus plant.
On Wikipedia page, they mentioned that Psyche butterflies larvae feeds on Capparis but Cardamine plants is not mentioned there. This is screenshot of Wikipedia page.
Invitation to join today’s CUBE chatShaala,

Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in all CUBE what’s app groups and on metastudio after the session

Here’s the direct link of the CUBE webinar: https://webinar.hbcse.tifr.res.in/b/kir-hye-cn4

Note: Today we are delaying the CUBE chatShaala for an hour

Timing:6.30-9.30pm
Looking forward to your participation
Thanks.
Attention!
For the Chai and Why? we need photos of homelab setup with the back of your home environment. As depicted in the sample picture that i have attached! The photos should reciprocate the idea that yes Homelab are actually at home and nothing sophisticated is required for science
Kind Request to all the CUBists to send the photos as soon as possible because we have to put that photos in the presentation for ‘Chai and Why?’ for the mock presentation that is tomorrow.

Thank you!

KiranKalakotiR · August 29, 2021, 7:59am

25th August 2021, 9.30pm to 26th August 2021, 9.30pm
Using potato, damp soil, damp tissue paper for observing nematodes.
[11:50 PM, 8/25/2021] Lydia cUBe Drosphila: That’s true
You have a mixed culture of young and old flies
Do you think you can plan something to find out how long fruit flies can live/their lifespan? What say?
Do you think knowing the lifespan of fruit flies is important?
[11:54 PM, 8/25/2021] Lydia cUBe Drosphila: Okay!
B19 indicates that this is the 19th culture bottle of single line B…right?
So you had B1, B2 and so on and now till B21…right? B1 started with 1 gravid fly…
And then their progenies are being transferred into the further culture bottles…right?
Just correct if I’m wrong anywhere
What happened to the initial culture bottles of single line B? Do you have any or do you only have the cultures B19, B20 and B21?
[11:55 PM, 8/25/2021] Lydia cUBe Drosphila: One more piece of information is required.
Can you please tell us the number of flies transferred to culture bottles B19, B20 and B21
[11:56 PM, 8/25/2021] Lydia cUBe Drosphila: What particularly in the smell of fermented yeast, attracted the fruit flies?
[11:58 PM, 8/25/2021] Lydia cUBe Drosphila: Nice!
Understood!
What is your objective with these mosquitoes?
(I’m sorry if I missed out on this too in the previous messages…not able to keep a track of all the messages these days)
Interesting!
A twist in the conversation! Which microorganisms live in the guts of ruminants? Maybe an example can help!
Also, can these microorganisms be used as cellulose digesters for some bio-based aspects or experiments ?
What do you think? Even I don’t know about this…I was just wondering about this while reading your message

Do we consume any food that has cellulose in it?

High levels of cellulose are found in root and leafy vegetables, legumes, and some fruits such as pears and apples
https://www.nap.edu/read/1222/chapter/13#293

Green beans were highest in cellulose and hemicellulose

We do consume some of these foods often. What do you think? How do we digest or consume these foods even though we don’t have the ability to do so? Won’t this be a problem ?
What do you think could be a reason for the death of fruit flies @Yash Sheregare CUBE? Even @Aswathy Suresh CUBE is facing the same issue.
[12:19 AM, 8/26/2021] Lydia cUBe Drosphila: These are working well @Yash Sheregare CUBE
SLPbB2 and SLPbC2 cultures
Then why is SLPbA2 having an issue?
[12:23 AM, 8/26/2021] Lydia cUBe Drosphila: This is good news! @Aswathy Suresh CUBE
Hoping to see and get more updates on this new A22 culture bottle
[12:25 AM, 8/26/2021] Lydia cUBe Drosphila: I think cardamine is a host plant for one butterfly species, right @Shraddha Sonavane CUBE?
That way we can connect I guess.
[12:27 AM, 8/26/2021] Lydia cUBe Drosphila: Yes
Life cycle of different model organisms can be connected…this comes under the curriculum topic, developmental biology
Yes @Lydia cUBe Drosphila, We can connect through butterfly Cardamine interactions.
Because Cardamine is host plants of Psyche butterflies.

VIDEO BY SEETHALAKSHMI AT 12.59PM
Update on ANT Model system

Immediative objective : To culture the Ants

Short term objective : To study it’s morphology.

Long term objective : How do ants communicate
Location : Pazhuvil
Date:25/8/21.
Time:9:51 pm
Data collector : Seethalakshmi
Have already sent earlier attached in drive
Some are given by me. Of phyllanthus and toddy. If needed, I will send more

Frugal HomeLab at South Mumbai, 26 August 2021
Nematode subcultureC started on 16thAug,21, 11days later 26/8/21Cubehomelab Kolkata BatulP
[9:43 AM, 8/26/2021] Arunan HBSCE: How do we Design an experiment to find the Lifespan of Fruitflies? @Aswathy Suresh CUBE @Lydia cUBe Drosphila @Ishita Assam @Raj Yashwantrao Patil CUBE @Yash Sheregare CUBE to design and start to show this today for Chai& Why Mock session.
[9:44 AM, 8/26/2021] Arunan HBSCE: What do we mean by fermented yeast? @Aswathy Suresh CUBE
Wow! Flowering of Mango trees observed at 10.5°N at Thrissur, Kerala.

@Aswathy Suresh CUBE What percentage of trees in this locality are flowering? @Aswathy Suresh CUBE
What is your further plan? @Yash Sheregare CUBE Please post a few good photos to show your HomeLab set up with Location and Date @Yash Sheregare CUBE
Ants release pheromones through which they communicate to fellow ants. That’s why ants move in a line…after one another.

During our childhood, we
used to disturb their sequence by moving fingers arround them. Ants used to disperse around and then again form a line.
Later on found out that we were dispersing the pheromones…disturbing their moving pattern…

It’s a very interesting phenomenon…
[9:56 AM, 8/26/2021] Arunan HBSCE: How about keeping a slide like this to show the extent of collaboration and a glimpse of concept map that all cubists work together, from across the country, to all at the end of Chai& Why? session, today?
[9:56 AM, 8/26/2021] Arunan HBSCE: @Saida Sayyed @KIRAN CUBE
[9:59 AM, 8/26/2021] Arunan HBSCE: Good, you transferred one fly to A22 bottle. Please tell us exactly what happened each day. @Aswathy Suresh CUBE
Excellent! @Man Masih Beck RANCHI CUBE Can you take videos of your activity like how you observe and Record the Data in your CUBE HomeLab from today as a video to be screened for Chai& We hy? session? @Man Masih Beck RANCHI CUBE Let’s start.[10:05 AM, 8/26/2021] Arunan HBSCE: Three larvae of SLBK A are great! Hope, you are following it up daily.

Please give update and post long-shot photos of your Lab Set up for Chai& Why? @Aswathy Suresh CUBE
[10:08 AM, 8/26/2021] Arunan HBSCE: Please take this observation asa movie how do you do at HomeLab. Let’s use it for Chai& Why to show how your lab world on a daily basis @Man Masih Beck RANCHI CUBE @Saida Sayyed
Excellent! @Aswathy Suresh CUBE What percentage of Mango trees in this locality is sprouting fresh leaves?
What percentage is flowering? Wonderful! @Aswathy Suresh CUBE
What percentage out of total trees are in this category? @Aswathy Suresh CUBE What is your Sample Size?
Beautiful! @Shraddha Sonavane CUBE
Can we develop this as a HomeLab experiment to show how exactly you
assemble all items to do this study for Chai& Why session? A movie will do. @Shraddha Sonavane CUBE Please collaborate @Aditiya Joshi CUBE @Lakshmy PJ CUBE @Shrudhiga
Surely more than 6persons eon Nobel Prize working on Fruitflies!@Yash Sheregare CUBE please clarify.
Long-shot photos to show the HomeLab set upar NewPanvel, please @Shraddha Sonavane CUBE
Please take a video on how do you do cold treatment to Cardamine seeds, showing your refrigerator, HomeLab set up for germination of seeds. @Arunima SN Nattika Cardamine Let’s use that for today’s hai &Why? mock session. @Arunima SN Nattika Cardamine
Please give a long-shot to show your Cardamine HomeLab set upay Kozhikode. @Arunima SN Nattika Cardamine
Date and also a long shotto show the HomeLab at your place @Sidhy Kerala Drosophila
Beautiful. Reference scale, Date,HomeLab location. Technique of Photography @Arunima SN Nattika Cardamine
Excellent! @Isha Pawle Can we have a on the spot movie of this transfer to show that we exploit the phenomena of Negative Geotaxis? @Ishita Assam
Dates and a few Long shots too, please @Arunima SN Nattika Cardamine
Longer shots with dates please! Wonderful😇 @Arunima SN Nattika Cardamine
We need to design a plan in order to find the life span of fruit flies - culture bottles ma’am.Here’s the short video of my fruit fly home lab.
From Worli, Mumbai.[10:42 AM, 8/26/2021] Aswathy Suresh CUBE: Definitely it’s important. Because Current situation facing major issue is flies dying in culture without any reason.we need to find out the answer. Before that we have to know how many days flies will alive (Life span)
[10:57 AM, 8/26/2021] Aswathy Suresh CUBE: Yes, B Line started on 26th May 2020 during the pandemic started time. started B line culture with 1 mother fly, trapped using tomato bait. Culture named SLBK B. Starting from there after several subculture passed B1,B2,B3,B4…B19, B20, B21 (subcultures) . Every months transferring all progeny’s from old media bottle to new media bottle in order to maintain that line culture. Last made subculture is SLBK B21 , Currently I have SLBK B18, B19, B20, B21 subculture bottles. But only B21 bottles have Larvae, Pupae, Adult Flies. Old bottles were taken for making new subcultures…cleaned and reused for making further subcultures (Because of unavailability of bottles).
[11:02 AM, 8/26/2021] Aswathy Suresh CUBE: I don’t have B subculture bottle which I started on 26/05/2020.That bottle were remained after 1 and half month. After that I taken that bottle and cleaned reused for making further subcultures.

Currently I have only B21 (Date of bottle : 12.08.2021) which have flies,larvae, pupae.
[11:03 AM, 8/26/2021] Aswathy Suresh CUBE: B Line
Great! @Isha Pawle We can make it shorter by editing some early part and show the long shot of the HomeLab setting. @Isha Pawle
Is this fine @Arunan HBSCE sir?
Total 5 mango tree surveyed. one of the mango tree started to flowering, another one started to sprouting. Rest of them (3 mango tree) no flowering, no sprouting, no fruiting.

26 August 2021. Fruit fly culture bottles in Home lab Thanniam,Thrissur, Kerala.
8 July 2020. Fruit fly Home lab Thanniam, Thrissur, Kerala. Sidhy.P.P , CUBE S N College, Nattika.
[1:15 PM, 8/26/2021] Arunan HBSCE: Looks fine. Last bit could be edited a few seconds more, I guess. @Isha Pawle
[1:16 PM, 8/26/2021] Arunan HBSCE: So what is the percentage calculation? @Aswathy Suresh CUBE
Can we get another still Long shot to see where in the house it is being maintained? @Sidhy Kerala Drosophila

Hello I am Binita. In June 2017 two months after returning back from HBCSE, I started first Kitchen lab. Undergraduate research is not yet popular in Jharkhand, so except @Dr. G C Baskey Cube Ranchi and @Shalini CUBE Rachi people were skeptical about undergraduate research. I wanted to check the hypothesis so I convinced my parents and family members. A space was needed for other people to discuss and perform science, we did several experiments in this space. 10 months after the lab started we got a space in college. If you ever thing that you are not able to do science because of some problem, then I am sure the problem in your head. Also I strongly believe that places and surrounding should not decide how fit we are for doing what we want to do. I got a chance to work in CCMB for master’s thesis in Dr. Rakesh Mishra’s lab. Currently I am taking a reading break, applying for PhD and running a VIRTUAL KITCHEN LAB. The idea is to gather people to share their journey in science. I hope you all will enjoy watching these videos. https://www.youtube.com/channel/UCXwh1LampcQbILWhqNmrynw There are some amazing videos that are coming up soon.
https://www.youtube.com/channel/UCXwh1LampcQbILWhqNmrynw
Home Lab - Bottukadavu Thrissur Kerala. Maintaining model organisms Fruit Flies, Moina.
[2:40 PM, 8/26/2021] Arunan HBSCE: Date please and repost with essential dryails. @⁨Aswath@Aswathy Suresh CUBE

[2:41 PM, 8/26/2021] Arunan HBSCE: details*
Date of photo : 26.08.2021
Time : 2:05pm
Photo taken : Aswathy Suresh CUBE SN Nattika.
Location : Bottukadavu - Thrissur Kerala.
Total surveyed Trees = 5
Flowering Mango trees = 1
Sprouting Mango trees = 1
Fruiting Mango trees = 0

% Flowering mango trees = 20%
% Sprouting mango trees = 20%
% No Flowering, Sprouting, Fruiting mango trees = 60%

Data Collector : Aswathy Suresh CUBE SN College Nattika.
Location : Bottukadavu - Thrissur Kerala.

A slice of the 1st day of CUBE National Meet 19, 20, 21March 2021 5-30pm to 9-30 pm https://webinar.hbcse.tifr.res.in/b/kir-vgc-6jf

https://www.youtube.com/metastudioCHAI AND WHY? SESSION
We are happy to announce that we have got the chance to be the part of Chai and Why?session organised by TIFR to share and narrate some of CUBE inventions that we came up collaboratively during the Pandemic .

Chai & Why? -TIFR’s unique outreach initiative since 2009 has been the “Chai and Why?” series, a forum to enable informal discussions of interesting scientific issues outside a traditional academic setting in a Science Café format.

We invite everyone to be the part of this session. The session would be conducted on zoom and would also be live telecast on YouTube

EVENT DATE: 29th August 2021
TIME: 11am to 12.15pm

Please reply to this message for more information.

Thanks😊
Great news👍
Very important sesions. Invitation to join today’s CUBE chatShaala,

Note: Today we are delaying the CUBE chatShaala & starting now.

Please join

Timing:6.30-9.30pm
Looking forward to your participation
Thanks.

Cardamine with developing floral bud for genetic engineering (floral dip method)dipping the floral bud in the solution containing genes.

A great achievement for our CUBE HOME LABS and CUBE CHATSHAALA as we completed a year today.
This is a histogram showing the number of participants who joined us each month from March 2020 to 18th March 2021.
How will Aditya isolate and characterize Yeast from fermented Coconut water?

Lab Assistants?
Which plates?

How the yeast colonies look?

Candida albicanshttps://en.wikipedia.org/wiki/Sabouraud_agar#/media/File:Candida_albicans_PHIL_3192_lores.jpg
Saccharomyces cerevisiae

Yeasts are eukaryotic, single-celled microorganisms classified as members of the fungus kingdom. The first yeast originated hundreds of millions of years ago, and at least 1,500 species are currently recognized.[1][2][3] They are estimated to constitute 1% of all described fungal species.[4]

Yeasts are unicellular organisms that evolved from multicellular ancestors,[5] Yeast - Wikipedia
Saccharomyces cerevisiae which is also known by its common name brewer yeast or baker yeast.
Boiled Potato slice, laced with S- shaped toddy ( fermented coconut water) trace after 2 days of incubation in room temperature on 22 July 2021 (at 15:50 hrs)), to isolate microbes that makes toddy from coconut water. CUBE Kishore Bharati HomeLab Initiative for Collaborative Science, Sophia, South Mumbai. Photo: mcarunan 22 July 2022

Will pasteurizing coconut water kill the spores also

Environmental and Genetic Determinants of Colony Morphology in Yeast Environmental and Genetic Determinants of Colony Morphology in Yeast
Baker’s yeast forms smooth round colonies when grown in favorable conditions. When starved for one or more nutrients, yeast can alter its growth pattern to produce complex structures consisting of numerous interacting cells. One mode of growth, the colony morphology response, produces visually striking, lacy colony architectures.

Uploading: WhatsApp Image 2021-08-26 at 9.18.46 PM(1).jpeg…

Why are some leaflets of Curry leaf plant showing axillary bud- like projections?

KiranKalakotiR · August 29, 2021, 7:59am

26th August 2021, 9.30pm to 27th August 2021, 9.30pm
Can someone share photographs of CUBE Lab, HBCSE, showing that even in labs we used to work with plastic bottles instead of beaker and flask !
This is for the Chai And Why?
!!!

Update on Fruit fly model
New Sub culture - SLPbA3, SLPbB3 and SLPbC3
Media Prepared on - 26/08/2021
Transferred Flies on - 27/08/2021 - 11:20am:11:30am :11:45am respectively

SLPbA3 - No of flies Transferred - 2
SLPbB3 - No of flies Transferred - ~ 30
SLPbC3 - No of flies Transferred - ~ 30
Location - Prabhadevi, Mumbai

Rechel’s CUBE Homelab, morabadi Ranchi

DATE:27/08/2/21

picture description

I Kept the setup in one of the corner of my room, currently having two model systems: Fruit fly & Cardamine
Materials visible in the picture :
(From left to right) washed bottles for fruit fly culture.
Stationeries, plastic cups, logbook, tool box ( dropper, cotton, laser light lens for microscopy, glass slide etc. inside it)
4 fruit flies culture bottles and plastic bottles.

Kept snake plant with the Home lab setup just for decoration.Can someone send good pictures of Phyllanthus plant!?
We should be able to see the hight and all in the photo.
This is for Chai and why!
@Shraddha Sonavane CUBE @Lakshmy PJ CUBE @Arunima SN Nattika Cardamine @KIRAN CUBE and others
Yes
Thank you @Janhvi Deshmukh
@Abhijeet Singh

VIDEOS SHARED BY ASWATHY AT 1.22PM
SLBK A22 (subculture of A line)…date of transfer 12.08.2021…transferred one fly.

16 days old culture bottle observed 2 flies (one is newly emerged) 2 pupae, 2 Larvae.

Update by : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu Thrissur Kerala.
SLBK B21 (subculture of B line)… date of transfer 12.08.2021…transferred 12 flies - ~5 gravid flies, ~7 male flies.

16 days old culture bottle observed ~20 flies, 50 pupae, 15 visible larvae.

Update by : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu Thrissur Kerala.

Rechel’s CUBE Homelab, morabadi, Ranchi

FRUIT FLY UPDATE
Date:27/08/2021

currently having 4 fruit fly culture bottles
RF-IIa T.D: 6/5/21. (1 fly)
RF-IIb T.D: 19/6/21 (~20 flies)
RF-IIc. TD: unknown (~50 flies)
RF-IIc". TD: unknown (~50 flies)

Good up: I didn’t mentioned the details (transfer date & media date) when I transferred into RF-IIc and RF-IIc, but I clicked the picture so that I could tag it later but didn’t find the picture in gallery.

History of the fruit fly culture

Trap kept on 09/03/2021 ( bait: chiku fruit peel & grapes) . Trapped 4 fruit flies on 11/03/2021.
Prepared TRSV medium on 11/03/2021 . I was able to transfer 2 fruit flies in the TRSV medium culture bottle (labbeled RF-II ) on 13/03/2021.
Media was prepared on 05/05/2021 Made subculture RF-IIa on 06/05/2021 . Transferred 4 flies from RF-II .
Media was prepared on 06/06/2021. Further subculture RF-IIb on 19/06/2021 . Transferred around 10 to 15 flies from RF-IIa .

5 . Further transferred fruit flies from RF-IIb to two another TRSV medium culture bottles. (Later labbeled as RF-IIc & RF-IIc" )

Further plan: to make TRSV medium to make single line cultureSLBK E17 (subculture of E line)… date of transfer 12.08.2021…transferred 14 flies - ~6 gravid flies, ~8 male flies.

16 days old culture bottle observed ~30 flies, ~60 pupae, ~20 visible larvae, ~25 dead flies.

Update by : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur Kerala.
Rechel’s CUBE Homelab, morabadi, Ranchi.

FRUIT FLY UPDATE
Date:27/08/2021
4 fruit fly culture bottles
RF-IIa T.D: 6/5/21. (1 fly)
RF-IIb T.D: 19/6/21 (~20 flies)
RF-IIc TD: unknown (~50 flies)
RF-IIc". TD: unknown (~50 flies)

Phyllanthus amarus plants grown in Panvel Home lab,location -Panvel, photo taken by- Shraddha, Date of photo-3/09/2020

ReMan’s Lab Ranchi 27-08-2021 Observing RNSL-B Fruitfly Culture Usually i click pictures in broad daylight. But when it’s a cloudy day like today, I have to do like this. Let’s find out #How_do_you_do_it? @Aswathy Suresh CUBE @Arunima SN Nattika Cardamine @Lakshmy PJ CUBE @Raj Yashwantrao Patil CUBE @Sachin Pradhan CUBE @Seethalakshmy CUBE and others…
Good idea

@Man Masih Beck RANCHI CUBE.
We can follow this method
Can you taje some close up pictures by using this method?

Actually my phone does not support closeup pictures. So to overcome that i use “jugaad” technique.[4:38 PM, 8/27/2021] +91 93247 51102: Species: - Phyllanthus urinaria .
Family: Phyllanthaceae .
Identifying features:-
Habit :herb(6-15m long)
Leaf shape: oblong ( pointed leaf).
Fruit surface :- rough.
Fruit colour : - green .
Placentation : - Axil.
Number of tepals :- 6.
Male and female flowers are present.
[5:10 PM, 8/27/2021] +91 93247 51102: Fruit surface is smooth for amarus .
In amarus number of petals are 5 .
Leaf base symmetrical in amarus.
Leaf apex : obtuse (round/ slightly point

KiranKalakotiR · August 29, 2021, 7:59am

27th August 2021, 9.30pm to 28th August 2021, 9.30pm
Nematode populations
We isolated 38 new strains of C. elegans and 25 new strains of C. briggsae from single wild hermaphrodites in Kenya and South Africa in March–April 2006. Despite extensive sampling from 36 sites, Caenorhabditis nematodes were only found in four locations: (1) compost from a private garden (Johannesburg, South Africa); (2) compost from a plant nursery (Ceres, South Africa); (3) compost from a mushroom farm (Limuru, Kenya) and (4) leaf litter in a public park (Nairobi, Kenya). Strain name designations for C. elegans are ED3040–ED3077, and for C. briggsae are ED3078–ED3102. The sampling sites and strain designations are described in Table 1. Various other nematode species were also found in these sites, including many strains of Oscheius tipulae, which have also been characterized (Baïlle et al., 2008).
Nematode populations
We isolated 38 new strains of C. elegans and 25 new strains of C. briggsae from single wild hermaphrodites in Kenya and South Africa in March–April 2006. Despite extensive sampling from 36 sites, Caenorhabditis nematodes were only found in four locations: (1) compost from a private garden (Johannesburg, South Africa); (2) compost from a plant nursery (Ceres, South Africa); (3) compost from a mushroom farm (Limuru, Kenya) and (4) leaf litter in a public park (Nairobi, Kenya). Strain name designations for C. elegans are ED3040–ED3077, and for C. briggsae are ED3078–ED3102. The sampling sites and strain designations are described in Table 1. Various other nematode species were also found in these sites, including many strains of Oscheius tipulae, which have also been characterized (Baïlle et al., 2008).
https://www.nature.com/articles/6801079/figures/1

[10:12 AM, 8/28/2021] Arunan HBSCE: C elegans in Africa @Batul Pipewala Kolkata @Saida Sayyed @KIRAN CUBE
[10:15 AM, 8/28/2021] Arunan HBSCE: Which is the latest publication on Indian Cenorhabditis? @Batul Pipewala Kolkata
[11:02 AM, 8/28/2021] Arunan HBSCE: “As a model organism, the nematode Caenorhabditis elegans has provided an outstanding system for elucidating fundamental problems in biology. However, the attitude regarding the natural history and ecology of Caenorhabditis has long been ‘hakuna matata’, to use the Swahili phrase meaning ‘no worries’. To achieve a robust understanding of the evolutionary context in which C. elegans’ developmental and genetic patterns emerged, it is necessary to characterize representative natural populations on a worldwide scale and to conduct comparative analyses of Caenorhabditis species.”
https://www.nature.com/articles/6801079#:~:text=As%20a%20model,of%20Caenorhabditis%20species.
Why hunting for Indian C elegans is important?

526th Day of CUBE Chatshaala: 27th August 2021
526th Day of CUBE Chatshaala: 27thAugust 2021 – Google My Maps
16 CUBists from 16 CUBE centres had joined the webinar
INDIA
Delhi
Dr P. Chitralekha: DSC

Jharkhand
Man Masih Beck: Kokar, Ranchi
Rechel Tirkey: Morabadi, Ranchi

Kerala
Arunima: Kozhikode
Aswathy Suresh: Thriprayar
Lakshmy PJ: Kandassankadavu
Seethalakshmi CR: Pazhuvil, Trissur

Maharashtra
Abhijeet Singh: Ulwe, Navi Mumbai
Aditya Joshi: Kalyan
Arunan MC: Breach Candy, South Mumbai
Khan Shaheen: NES Ratnam College, Mumbai
Manasi …
Latest map from where C elegans has been found green dots and pink areas show where C elegans has not been found despite sampling.white areas show where sampling for C elegans has not been done

[12:13 PM, 8/28/2021] Batul Pipewala Kolkata: Article by Marie Ann Felix
[12:35 PM, 8/28/2021] Batul Pipewala Kolkata: Outstanding questions about the natural history of C. elegans.

What is the pattern of C. elegans migration at different geographical distances? What are the relevant vectors? Are humans now major vectors over large scales?

Over a small scale, ongoing local population surveys will provide insights into migration patterns. Over large scales, selective sweeps estimated to date from 100–200 years ago have spread to different continents: their migration and selection might thus relate to human activity (e.g., via human travelling and the trafficking of goods, and via selection, perhaps by chemical compounds) (Andersen et al., 2012).

What is C. elegans geographical center of origin? Are there species that are very closely related to C. elegans (see phylogeny in Box 2)?

Increased sampling in poorly sampled and isolated geographical regions might provide access to divergent populations that did not undergo the recent selective sweeps and so inform us of C. elegans’ older history and habitats.

What does C. elegans eat?

C. elegans likely feeds mostly on bacteria, but we do not know which bacteria. The sterols it requires might come from yeast or from the rotting plant substrate itself.

What is the generation time and the number of generations per year in the wild?

The latter is surely far less than in the laboratory (ca. 150), but is it by one or two orders of magnitude?

What are the relevant dauer entry and exit cues?

Whether associated organisms have an influence on dauer formation has not been investigated.

Where does C. elegans spend its winter? What are the source populations?

In temperate regions, C. elegans is most often sampled in autumn, when it is found at high density. We ignore how and where this organism survives winter, for example, whether it becomes associated with a carrier organism.
Last week we had a wonderful conversation on Indian Philosophies. We will continue our barefoot chat live session-32, this afternoon from 3-4 pm at https://webinar.hbcse.tifr.res.in/b/dp6-vs2-a2s

More Details: BFC Session-32 [August 28 2021] Order and Chaos in our physical and social worlds
@Seethalakshmy CUBE, @Raj Yashwantrao Patil CUBE @Shrudhiga

VIDEO SHARED BY SANA AT 3.37PM
last year video (4/11/2020)
objective of the study:To replicate nutrient broth made in labs and inoculate microbes

Observation:A thick biofilm on home made lab
Long term objective:To grow bacteria in our home by using frugal Nutrient broth.
Home lab:Sana Jalili,Mira road
What is the frugal nutrient broth you made at home
How did you make nutrient broth in HomeLab? Please describe.
Invitation to join today’s CUBE chatShaala,
Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in all CUBE what’s app groups and on metastudio after the session

Here’s the direct link of the CUBE webinar: https://webinar.hbcse.tifr.res.in/b/kir-hye-cn4

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.

VIDEOS SHARED BY SUSANTA
Half an hour time lapse video of Sleep Wake Cycle : Phyllanthus
Date :28 August 2021
Time : 5.07 PM to 5.37 PM
Location :Sonari, Assam 27°N95°E video :Susanta Tanti

PHOTOS SHARED BY ADITIYA AT 7.20PM
Microbial colonies in less than 24 hours on boiled potato slice
GENTLE REMINDER to join tomorrow’ CHAI AND WHY? session at 11am

Date: 29th August 2021 (Sunday)
Time: 11am

Youtube link: https://youtu.be/lqUTobuDgvs

FaceBook link: Redirecting...

Looking forward to your participation
Thanks
Following are the links to spread the event.

Zoom link: Launch Meeting - Zoom

Youtube link: https://youtu.be/lqUTobuDgvs

FaceBook link: Redirecting...
All the best everyone. Rock it tmrw. Let the ripples be felt widely.
All the best to all teams!!!
Quite excited…all the best everyone…looking forward👍🏻
All the very best to Everyone and each team
Let’s have Chai and Ask Why tomorrow!
[10:22 PM, 8/28/2021] Priti Kanade: All the Best:+1:t2:
[10:23 PM, 8/28/2021] Priti Kanade:
All the best
All the best give your best shot everyone!!

KiranKalakotiR · August 29, 2021, 8:00am

28th August 2021, 9.30pm to 29th August 2021, 9.30pm
Following are the links to spread the event.

Zoom link: Launch Meeting - Zoom

Youtube link: https://youtu.be/lqUTobuDgvs

FaceBook link: Redirecting...
All the best everyone. Rock it tmrw. Let the ripples be felt widely.
All the best to all teams!!!
Quite excited…all the best everyone…looking forward👍🏻
All the very best to Everyone and each team
Let’s have Chai and Ask Why tomorrow!
[10:22 PM, 8/28/2021] Priti Kanade: All the Best:+1:t2:
[10:23 PM, 8/28/2021] Priti Kanade:
All the best
All the best give your best shot everyone!!
527th Day of CUBE Chatshaala: 28th August 2021
527th Day of CUBE Chatshaala: 28thAugust 2021 – Google My Maps
31 CUBists from 31 CUBE centres had joined the webinar
INDIA
Assam
Ishita Sonowal: Jorhat
Krishnendu Roy: Silchar
Susanta Tanti: Sonari

Bihar
Aryan Kumar:Sitamarhi

Delhi
Aastha Ahuja: ANDC
Dr P. Chitralekha: DSC
Raj Yashwantrao Patil: New Delhi

Jharkhand
Binita Ghosh: Ranchi
Man Masih Beck: Kokar, Ranchi
Rechel Tirkey: Morabadi, Ranchi

Kerala
Aswathy Suresh: Thriprayar
Lakshmy PJ: Kandassankadavu
Seethalakshmi CR: Pazhuvil, Trissur

Maharashtra
Abhijeet Singh: Ulwe, Navi Mumbai
Aditya Joshi: Kalyan
Arunan MC: Breach Candy, South Mumbai
Gaurangi Dhuri :Tilaknagar, Mumb…

Click here for more info

GENTLE REMINDER to join today’ CHAI AND WHY? session at 11am

Date: 29th August 2021 (Sunday)
Time: 11am

Youtube link: https://youtu.be/lqUTobuDgvs

FaceBook link: Redirecting...

Looking forward to your participation
Thanks
We are live, it’s live now
CHEERS EVERYONE
Very well done, Saida, Man, Binita, Arunan and all the cubists.
Amazing chai and Why session.
Amazing Session:confetti_ball:

A proud CUBIST

There may be many who may have missed the event live, do send the the youtube link widely.

Congratulations to all of us
Great collaboration
Yes
Yeah!
Session went really well…and especially without any delay!!!
Attended on youtube. Fantastic session!
Great work everyone!
Congratulations and celebrations
Thanks @Shivam CUBE NEMATODE
@Binita Cube isn’t going to like this screenshot for sure.
I enjoyed it , it was a good session , congratulations to all
Amazing work !congrats!!
Wow
Thank you @Arunan HBSCE for the kind and caring alarm ‘comment’.
@Binita Cube
What a feast
Indeed

he never miss doing that

these screenshots are inevitable.
What a feast. Mind you, it is just the beginning and surely a great beginning. Many great discoveries, indeed.
[12:47 PM, 8/29/2021] +9150803: Yeah! He is
[12:47 PM, 8/29/2021] +91 50803: Hahaha
[12:47 PM, 8/29/2021] +9150803: Oh Captain, my captain !!
You are the sail @Rahul Kushwaha
We will keep doing more chai and why sessions, next time on a specific activity.
That’s a great idea. We actually have enough flesh in all our projects. And also multiple students doing it. It’s time to develop now on individual projects as well.
Some more screenshots of Chai and why? Session
Poll results
Great collaboration…
Great show by all the enthusiastic cubits…
Excellent session Cubists…
Amazing session
Congratulations to all!

This is only the beginning!

Please join in today’s CUBE Chatshaala to celebrate and review.

VIDEO SHARED BY MAN AT 5.39PM

Late Post Breaking News
Flies appeared after 9th day of transfer

CUBE Ranchi Re-Mans’ Home Lab
28-08-2021
Fruitfly Update

Immediate Objective: to start a single line fruitfly culture(RNSL-B) at ReMan’s Lab in TRSV Media

Short Term Objective: -To identify the trapped fruitflies
-To get native Drosophila melanogaster species

Media preparation- 18-08-2021
Transferred date- 19-08-2021

OBSERVATION
after 9 days of transfer
•Larvae ~10
•Pupae ~50
•Fly ~8 to 10

Expectation vs Reality
Earlier expectation was that New flies will emerge on 27th Aug, 2021 but they emerged on 28th Aug instead

Goof-up
I had not mentioned the time of transferring fruitflies that was on 19th Aug at around 5 pm.
@Aswathy Suresh CUBE @Yash Sheregare CUBE @Lydia cUBe Drosphila and others…

KiranKalakotiR · August 29, 2021, 8:00am

29th August 2021, 9.30pm to 30th August 2021, 9.30pm

@Aswathy Suresh CUBE @Arunan HBSCE

Mango tree not started flowering!

Flowering not started
No sprouting
No Fruiting Yet.

Date : 25.08.2021,
Time : 01:47 PM

Latitude: 19.0760° N,
Longitude: 72.8777° E

Temperature :
Maximum : 25°C
Minimum: 32°C

Location: Somaiya college, Vidyavihar, Mumbai Maharashtra
CUBE Homelab Vidyavihar
Data Collector: Abhijeet Singh
Mango tree not started flowering!

Flowering not started
No sprouting
No Fruiting Yet.

Date : 27.07.2021,
Time : 05:08 PM

Latitude: 26.6438° N,
Longitude:84.9040° E

Temperature :
Maximum : 26°C
Minimum: 35°C

Location: Motihari, Bihar
CUBE Homelab Motihari
Data Collector: Arshad Shaikh
Aparna K Dinesh -
SN college Nattika
Noted. Thank you!

29th August 2021 CUBE Chatshaala - White Boards .

@Rechel Turkey Cube Ranchi Moina Please add your summary about your design of experiment, How to study Life span of moina.
Please give the location first before giving Longitude & Latitude @Abhijeet Singh

29/08/2021 Chatshala summary

In yesterday’s chatshala, we started discussing with the microscopic picture which was shared by @Navneel from Kolkata. We were trying to identify that organisms which was looking very similar to Moina and daphnia.

We also discussed classification of fruit fly and moina/daphnia so that we can differentiate between them on the basis of morphological characteristics.

@Batul Pipewala Kolkata told the story of identification of moina in CUBE by @kajal.

in Ranchi we were culturing Moina since August 2019 and lost Moina in July 2021. Which was the same well studied Moina macrocopa JSK1 which was brought by Sweta and Manjuel after August, 2019 CUBE national meet from CUBE, HBCSE, MUMBAI.

Discussed about the standard protocol for moina culture , we culture Moina in container containing 250ml dechlorinated water (DC water)and feed them 1 drop of milk everyday . We feed milk drops, so that milk bacteria will grow and moinas will feed on them.

@Soumyadip from Kolkata also told that he was also having crustaceans something like daphnia /Moina when he was culturing Azola .

Then we discussed about the life cycle and life span of Moina . So in order to check the life span we tried to set up an experimental design.

according to the hypothetical experimental design to check life span of Moina, 1st we need to identify the adult gravid Moina that can give birth to baby moinas.

Then we can isolate them in a container with 250ml DC water and feed them 1drop milk everyday day.

When the mother Moina will give birth we will get day1 moina . Then mother Moina will be separated out and we can observe day 1 moina for further observation.

When these moinas will become adult and gravid and produce baby Moina we will keep separating out new baby Moinas and we will keep observing them until they die.

                   @Arunan HBSCE @Aswathy Suresh CUBE @Batul Pipewala Kolkata @KIRAN CUBE @<U+2068>Navaneel<U+2069> @Man Masih Beck RANCHI CUBE @Janhvi Deshmukh @Saida Sayyed @Arunima SN Nattika Cardamine  and others

Added Navneel St Xavier’s College, Kolkata.
Great!
Please repost just the Design of Experiment for studying lifespan of Moina @Rechel Turkey Cube Ranchi Moina
Thank you sir.
[1:21 PM, 8/30/2021] Arunan HBSCE: Please start posting photo updates of each of our model systems. @Aswathy Suresh CUBE @Yash Sheregare CUBE @aditya and others.
[1:26 PM, 8/30/2021] Arunan HBSCE: Please write a short summary with a photo of your work @Navaneel

Present image of the organism under discussion. Data collector Navneel CUBE St Xavier’s College, Kolkata
Can see 7 baby daphnia like organisms inside @Navaneel
Hello everyone, thank you for including me in the discussion. I am Navaneel Sarangi, from St. Xavier’s College Kolkata. Project details : Experimental observations of Pondwater sample from Saltlake, Kolkata -

Objectives : Immediate Objective : To establish microscopic biodiversity of single Pondwater samples from Saltlake, Kolkata. (I have not decided whether to focus on a particular phylum)

Short term Objective : To analyse methods of enrichment of pondwater samples, as well as isolation of organisms of interest followed by culturing of the same.

Long term Objective : To establish taxonomic microbial biodiversity of pondwater samples (multiple) from Salt Lake Kolkata. Database setup with nomenclature annotation of samples visualized. Establishment of protocols for enrichment of pond water samples for microscopic analysis. Establishment of protocols for isolation of organisms of interest followed by culturing of the same.

Apart from this, I am an aspiring astrobiologist, and involved in different research activities through my position of VIsiting Scholar at Blue Marble Space Institute of Science. However, any and all aspects of science interest me, especially those we can perform without the complications of an institutional laboratory.
Micro biology and Space great combination:ok_hand:
What is the actual size of this waterflea? @Navaneel
Hello Navaneel,

Really appreciate the detailed note of your immediate, short term and long term objectives regarding the culture and maintenance of aquatic microorganisms.
I have some questions regarding the same:

What do you mean by establishing microscopic biodiversity? I mean isn’t it already established in the natural source? (Salt lake) do you mean culturing?
What do we mean by analysis methods of enrichment of samples? A more simple elaborate sentence will be better for someone like me to understand the motive.
Is Salt Lake, kolkata a source of freshwater body? Why is the name salt lake ?

VIDEOS SHARED BY ASWATHY AT 3.41PM
SLBK A 22 - single line sub culture bottle of A Line… Date of transfer flies 12.08.2021.

Transfer no of flies : 1

Observations on 18th Day
30/08/2021
0 Eggs
1 Larvae (3mm)
0 Unhatched Pupae
1 Fly
1 Dead Fly

Update : Aswathy Suresh CUBE SN College Nattika.
SLBK B 21 - single line sub culture bottle of B Line… Date of transfer flies 12.08.2021.

Transfer no of flies : 12, Gravid flies : 6, Male flies : 6

Observations on 18th Day
30/08/2021
~10 Larvae (3mm)
~40 Unhatched Pupae
~20 Flies
~20 Dead Fly

Update : Aswathy Suresh CUBE SN College Nattika.
SLBK E 17 - single line sub culture bottle of E Line… Date of transfer flies 12.08.2021.

Transfer no of flies : 14, Gravid flies : 6, Male flies : 8

Observations on 18th Day
30/08/2021
~40 Larvae
~60 Unhatched Pupae
~8 Flies
~30 Dead Flies

Update : 30/08/2021 ; 3:49pm
Update by : Aswathy Suresh CUBE SN College Nattika.
What is your research project at BMSIS? CUBE is not limited you can discuss your astrobiology project here too
Please give a summary of your Single Line Cultures as BREAKING NEWS @Aswathy Suresh CUBE @Yash Sheregare CUBE @Isha Pawle @Sidhy Kerala Drosophila and others.
How many are doing good?

A better sentence would be to analsyse the microscopic biodiversity, and classify the same according to taxonomical heirarchy. Thank you for pointing it out.
Pond samples are a real-word reflection of microbial communities. These ecosystems have unqiue interactions, which control the population of each niche present. What factors help in incresing the general population of microbes in the medium ? What factors influecne particular niches ?

Eg : If we observe a community of Paramecium in our sample, can we selectively enrichen the paramecium community ?

SaltLake is a Municipality division in Kolkata, easily demarcated from the rest of the city. It used to be a part of the East Kolkata Wetlands, which still supports a brackish ecosystem. It was filled up in the 1950s to build one of the first planned cities of India. You can find more information here : http://www.ekwma.in/ek/wp-content/uploads/2015/09/NewsletterVolume1.pdf

image1280×621 101 KB

Location for sampling
CHAI AND WHYCUBE presentation showed that it is possible to do research on biological research model systems at home using easily available cheap alternatives by understanding the science behind it.
Please give your views on the presentation @Navaneel @Lakshmy PJ CUBE @Arunima SN Nattika Cardamine @Aswathy Suresh CUBE @Sana Institute Of Science @Dr Chun Mei Chang @Sunita Chahar CUBE
What is the source of water?Is it the municipal taps?
Yes. But I dont think it is unified with he central distribution system of saltlake

I have a question. The modified microbial culture media using coconut water, what were te constituents and standardisation of the same?

VIDEO SHARED BY SEETHALAKSHMI AT 6.17PM
𝗨𝗽𝗱𝗮𝘁𝗲 𝗼𝗻 𝗔𝗻𝘁 𝗠𝗼𝗱𝗲𝗹 𝘀𝘆𝘀𝘁𝗲𝗺

Date of photo : 30/8/21.
Time: 3:23pm.
Date of setup :25/5/21.
Location : Pazhuvil.
𝗜𝗺𝗺𝗲𝗱𝗶𝗮𝘁𝗶𝘃𝗲 𝗢𝗯𝗷𝗲𝗰𝘁𝗶𝘃𝗲 : To culture the ants.
𝗦𝗵𝗼𝗿𝘁 𝘁𝗲𝗿𝗺 𝗼𝗯𝗷𝗲𝗰𝘁𝗶𝘃𝗲 : To study it’s morphology.
𝗟𝗼𝗻𝗴 𝘁𝗲𝗿𝗺 𝗼𝗯𝗷𝗲𝗰𝘁𝗶𝘃𝗲 : How do ants communicate.

Size of ants:2.3-3 mm
[7:29 PM, 8/30/2021] Batul Pipewala Kolkata: @Aditiya Joshi CUBE please answer his query
[7:29 PM, 8/30/2021] Batul Pipewala Kolkata: Is it unfiltered water from ganga?
Navaleen: Yes I think so. It is from the older pipelines which were used to dredge

VIDEO SHARED BY SEETHALAKSHMI AT 8.34PM
Close - Up view of my culture Ant, ( By using magnifying lens.)
Feeding sugar syrup
Location: Pazhuvil
Date:30/8/21
Time:3:44pm
Data collector : Seethalakshmi
@Navaneel I used boiled potato slice as medium. I just spread the fermented coconut water on that slice.
[8:47 PM, 8/30/2021] +91 98308 28389: Can we use the coconut water as a broth for culture
[8:48 PM, 8/30/2021] +91 98308 28389: Maybe use it with rava to concote an alternative to agar ? I am not very sure of that tho.
[8:52 PM, 8/30/2021] Aditiya Joshi CUBE: Yes. I think we can.
[8:53 PM, 8/30/2021] Aditiya Joshi CUBE: Yes we can surely try it. Like in TRSV medium prepared but cubists.
[9:26 PM, 8/30/2021] +91 28389: https://www.gesundheitsindustrie-bw.de/en/article/news/the-discovery-of-homeotic-genes
[9:27 PM, 8/30/2021] +91 28389: Please check the link for homeotic genes

Update on cardamine model system.

Location : Pazhuvil.
Date:30/8/21.
Time : 4:53 pm.
Data collector : Seethalakshm

KiranKalakotiR · August 31, 2021, 11:50am

30th August 2021, 9.30pm to 31st August 2021, 9.30pm

How do you plan to study it’s morphology?
Start with objective @Seethalakshmy CUBE !
What do want to show us through this picture!?
Amazing capture @Seethalakshmy CUBE !

I think during Chai and Why? We lost the account of your identification studies.

Were are we in the identification of your ants!

Also @Gaurangi Dhuri has some similar interests as what your long term objective suggests! Please collaborate!
Chai and Why … presentations were amazing and impactful. How students have made home labs and doing research during pandemic…was so inspirational. Congratulations to everyone for the efforts and passion in science.
[11:04 PM, 8/30/2021] Seethalakshmy CUBE: Our plan is,
find immobilized ant from our culture( or catch an ant and kept in refrigerator ). Looking their body parts by using phone microscopy.
@Anjani Kumar Kashyap CUBE
[11:08 PM, 8/30/2021] Seethalakshmy CUBE: Ok @Abhijeet Singh!
my objective is To culture the Cardamine in my homelab.
Actually this is not a good culture.
Because the cardamine shows less growth as well as some plants are died.
I want to overcome this.
[11:10 PM, 8/30/2021] Seethalakshmy CUBE: Thank you!@Abhijeet Singh
Yes! you are right! We want to restart this.
[11:13 PM, 8/30/2021] Seethalakshmy CUBE: Sure! Let’s discuss collaboratively!
Yes @Gaurangi Dhuri
Please ask questions as well as tell us your ideas!
Have you noticed any specific morphological feature? Which order it belongs to?
They are coming under order Hymenoptera @Dr. Sarita kumar Delhi CUBE ma’am
Any specific character of Hymenopteran? Have you ever observed wings in ants like present in other Hymenopterans such as bees, wasps…

hello navaneel.I appreciate the work in your home lab:tada: Keep it up!!
I am interested to know about the

Microbial diversity you are finding out.
3.Why only salt lake as a source of marine ecosystem ?
What is your expected results.
3.Also I would like to know the detailed observation you got from daphnia photo
4.What is nomenclature annotation that you mentioned

@Navaneel @Batul Pipewala Kolkata @Saida Sayyed @Arunan HBSCE and others
What do you mean by immobilized ant? What benefit does that give over any non immobilized ant?
Yess…
[8:43 AM, 8/31/2021] Seethalakshmy CUBE: Yes @Dr. Sarita kumar Delhi CUBE ma’am
Hymenoptera have 2 pairs of wings (fore wings and hind wings) the fore wing is long compared to hind wing and membranous
[8:51 AM, 8/31/2021] Seethalakshmy CUBE: Immobilized means immovable / dead ant!
[8:54 AM, 8/31/2021] Seethalakshmy CUBE:

Expt documentation : Study of microbes in tap water from saltlake kolkata.
[9:40 AM, 8/31/2021] +9128389: Tap water is a common source of drinking water. In this expt, I have used coconut water (5ml ) with an ear bud full of tap water inoculum, kept in the blue lid urine collection cup. I will check for microbial growth 24 hours later. The vessel has been sealed with parafilm to prevent contamination. The urine cup seal was broken 10 mins prior to start of experiment.

To check for direct microbial growth, I envisioned potato surface based microbial growth. Here in the picture, we have a control potato slice with no inoculum on the right. The plate has been sealed with parafilm.

On the left we have the experiment potato plate. The inoculum has been streaked with an ear bud in a z pattern of continuous streaking.
[9:42 AM, 8/31/2021] +91 8389: In the absense of an autoclave, all the instruments were sterilised with home made 70% ethanol and flamed with a gas lighter. Heat sensitive material such as plastic was thoroughly cleaned with ethyl alcohol. To prevent airborne contamination, the exposure of experiment surface to air was minimised as much as possible.

Coconut water broth was collected 20 mins prior to the experiment.
Please give essential description namely Objective, Location, Date, Name of photographer along with each photo. @⁨Navnee@Navaneel
Objective : Study of microbes in Tap from Saltlake, Kolkata
Location : Saltlake, Kolkata
Date : 31/08/21
Photographer : Navaneel Sarangi
[10:03 AM, 8/31/2021] Arunan HBSCE: Plese give one or two examples from the presentation to initiate discussion @Batul Pipewala Kolkata
[10:05 AM, 8/31/2021] Arunan HBSCE: To me, it looks like gutter or drainage water, greeting stuck. @Batul Pipewala Kolkata @Navaneel
I wish it was sir. It is actually a prominent water source for the surronding market. I was very suprised to observe that
Are you @Navaneel asking about the medium used for streaking the fermented coconut water by Aditya @Aditiya Joshi CUBE?
Yes sir.
[10:11 AM, 8/31/2021] Arunan HBSCE: What is the summary of your culture method of these ants in your home lab? @Seethalakshmy CUBE
Are you recommending this method to others and are you inviting others to be collaborators? Please clarify and give a short summary from 25 May 2021 onward. Great to see this.@Seethalakshmy CUBE
[10:14 AM, 8/31/2021] Arunan HBSCE: @Seethalakshmy CUBE But you have not mentioned the name of these ants which you are culturing at your Pazhulvil HomeLab from May 2021.
Any idea which ants are these?
Common name along with vernacular name and reference to verify this, please. @Seethalakshmy CUBE
[10:15 AM, 8/31/2021] Arunan HBSCE: Please give long shot pictures of this site please @Navaneel
What type of magnifying lense? @Seethalakshmy CUBE
What is the size of these ants? @Seethalakshmy CUBE Why no reference item for measurement?
Are we not to give some reference on size?
Are we not interested to give some details about the type of ants that you are posting pictures of like whether these are all worker ants? @Seethalakshmy CUBE
Or is there a queen ant in this photo?
[10:21 AM, 8/31/2021] Arunan HBSCE: What progress did you make in the study of these ants in the last three months, from May 2021? @Seethalakshmy CUBE
[10:22 AM, 8/31/2021] Arunan HBSCE: @Aditiya Joshi CUBE A great opportunity to give photo updates with a summary since people are interested in your work @Aditiya Joshi CUBE
[10:23 AM, 8/31/2021] Arunan HBSCE: Please elaborate to continue discussion and for further development @Aditiya Joshi CUBE @Navaneel
Since you are finding microbes from tap water why are you streaking with coconut water.Another question if you make it airtight will it not increase proportion of anaerobic organisms and kill the aerobic ones?@Navaneel @Aditiya Joshi CUBE
[10:23 AM, 8/31/2021] Arunan HBSCE: Please elaborate your idea and a Design for the same @Aditiya Joshi CUBE
[10:28 AM, 8/31/2021] Arunan HBSCE: Great! @Navaneel

Please give a short description to inspire readers, to start discussing. @Navaneel

Anything interesting did you personally find, in this reference!
Please give a short description at least, as a summary with your objectives to inspire readers, to discuss further.
@Seethalakshmy CUBE
Why are we interested in Cardamine? @Seethalakshmy CUBE
How are you sure these are Cardamine? @Seethalakshmy CUBE
[10:33 AM, 8/31/2021] Arunan HBSCE: What is the culture condition? @Seethalakshmy CUBE
Why so few seedlings?
What is your objective?
Let’s inspire others to collaborate, by giving essential information.
[10:35 AM, 8/31/2021] Arunan HBSCE: Absolutely @Abhijeet Singh
Please give us the short story, starting with your objective @Seethalakshmy CUBE
[10:37 AM, 8/31/2021] Arunan HBSCE: What is the purpose? @Seethalakshmy CUBE
Are all ants from the colony be alike?
[10:38 AM, 8/31/2021] Arunan HBSCE: Please give the details @Seethalakshmy CUBE
Let’s all join after knowing your objectives.
[10:39 AM, 8/31/2021] Arunan HBSCE: Any reference and any further reasons to believe that these are hymenopterans? @Seethalakshmy CUBE
[10:42 AM, 8/31/2021] Arunan HBSCE: Reference please. @Seethalakshmy CUBE

Quarantine Mood Sparing breakfast(Upma) with fruit flies

. Successfully trapped two fruit flies (one is gravid) today in the morning at 7am. Date of photo: 31st August 2021
[10:47 AM, 8/31/2021] Arunan HBSCE: Please include objective in each post like this @Navaneel
Any BREAKING NEWS from your Salt Lake HomeLab?
[10:50 AM, 8/31/2021] Arunan HBSCE: What do you mean by prominent water source? Do people use the water retained in the tank for daily use?

Or, people use the running water from the tube into the drainage looking pit for drinking etc. Please clarify.
[10:51 AM, 8/31/2021] Arunan HBSCE: What is the exact source of your water for water fleas and paramecium etc., shown in some earlier pictures? @Navaneel
[10:52 AM, 8/31/2021] Arunan HBSCE: Please clarify to continue discussion with @Aditiya Joshi CUBE and others. @Navaneel
[10:58 AM, 8/31/2021] Arunan HBSCE: GREAT to see HomeLab at Homi Bhabha Center🤣 Almost scandalous😌 @KIRAN CUBE
Looking forward to your Quarantine Labwork @KIRAN CUBE

Please repost the photo with corrections.
[11:11 AM, 8/31/2021] +91 28389: ok sir
[11:11 AM, 8/31/2021] +91 28389: Yes sir we are
[11:12 AM, 8/31/2021] +91 28389: Not streaking with coconut water. I am using coconute water as a broth to culture any microbe in the tap water. If the quantity of microbes is less, then we may not get any colonies on the potato, but the coconut water may enrichen the same
[11:15 AM, 8/31/2021] +91 28389: For short term growth like 24 hours, my previous laboratory experience has been that there is no drop in optimum growth of culture for aerobic microbes. But as you mentioned, I may do a comparative study of sealing them and not sealing them to see if there are any changes.

[11:22 AM, 8/31/2021] Arunan HBSCE: Please be careful because you are possibly enriching microbes from elsewhere:face_with_monocle:, like from tap water using nutrient rich coconut water. @Navaneel
What are your EXPECTATIONS?

Please explain your DESIGN fully to be aware of possible consequences.
[11:25 AM, 8/31/2021] Arunan HBSCE: Don’t you expect any pathogenic bacteria being enriched in this process?
Let’s write down the full Design clearly and discuss the consequences. @Navaneel

Do you see a difference between that of @Aditiya Joshi CUBE and of your Design of HomeGrown Microbes? @Navaneel
Design of the experiment :

an earbud soaked in tap water is immersed into 5ml of Coconut water. The experiment is being carried out in a safe enclosed environment, adjacent to a doctor’s chamber.

Expectations : There should be no microbes present in the tap water submerged culture as it is chlorianted by the Municipality. Likewise, the surface culture on potato should not yeild any bacterial isolates.

Alternate Hypothesis : If there is presence of colonies of microbes on submerged or surface culture, there is contamination of microbes in the tap water being used for drinking resources.

Since most of us use this water in one way or the other without additional filtering, and there have been no untoward incident so far, I expect the microbes been enrichened are not pathogenic.

Opening and closing of leafs pattern observed in Chini Gulab or Sun Rose . Botanical name:- Portulaca grandiflora My mother brought this plant from nearby nursery two weeks before. I observed the leafs showing some closing and opening kind of pattern. So to look into it I took a photo yesterday in the evening and a photo this morning. I can clearly see that the plant’s leafs are opening and closing. Compare the two photos. What do you see @Lakshmy PJ CUBE @Arunima SN Nattika Cardamine @Aswathy Suresh CUBE @Arunan HBSCE @Batul Pipewala Kolkata @Aditiya Joshi CUBE . Can we also developed Chini gulab as a model for sleep wake studies in Phyllanthus??? Please post your comment and help me do this! Looking forward…!Yes sir. In case Aditya’s experiment design, the microbes being enrichened are directly derived from the fermented coconut broth. Since the coconut broth is usually consumed in some manner or the other, the organisms are not pathogenic.

In my case, I am not using the coconut broth itself as the isolate, but as a medium for enrichment.
How does Doctors’ chamber help?
The doctor’s chamber provides a sterile environment to work in and has air flow systems isolated from any living area. All air inlets are fitted with HEPA filters. I myself use a lab coat, sterile nitrile gloces and mask while processing any sample. I make sure to sterilsie all and any surface with 70% ethanol before and after the experiment.
[11:31 AM, 8/31/2021] Arunan HBSCE: Why do you doubt? @Abhijeet Singh
Time in AM & PM is essential for getting clear idea.
Please give essential details
[11:34 AM, 8/31/2021] Arunan HBSCE: Do you expect to get collaborators, if your initial requirements are this stringent? Can’t we do microbial research without such demanding requirements? @Navaneel
[11:35 AM, 8/31/2021] +9128389: yes sir, that is some additional precaution. Tap water shouldnt have any pathogenic microflora, and rhizobial cultures can also be processed without any of the above requirements. In fact i used to do the same, but due to the worry of my parents (who are doctors) I had to move to the same area
[11:35 AM, 8/31/2021] +91 28389: However, the requirement of gloves and ethanol or any sanitiser must be there
Why do you need coconut water to enrich them will not boiled potato itself provide nutrients to the microbes?
They are two seperated mediums mam. One is a submerged culture medium (Coconut water) and the other is a surface culture medium. Often, especially in the laboratory, we first perform a submerged culture to increase the concentration of microbial cells before following through with a surface culture.
Let’s move forward step by step. @Navaneel
What is your research question?
Let’s clarify and proceed.
Research Question : Are there any microbes coming with the municipality tap water
Details:-
Time of pic on left side :- 8.35 AM
Time of pic on right side:- 5.40PM

Place:- Ulwe,Navi-Mumbai.

This is a boiled potato slice showing growth of bacteria from just air after 24hrs@@Navaneel @Aditiya Joshi CUBE
yes that is why i had to seal the potato in. Otherwise there would have been air borne contamination
Correction @Navaneel On this boiled potato slice I had transferred nematodes with two drops of tap water.Nematodes also have bacteria attached to their bodies and tap water bacteria are also present besides air.
[11:58 AM, 8/31/2021] Batul Pipewala Kolkata: Sorry there is a mistake ,Correction given above
[12:02 PM, 8/31/2021] Batul Pipewala Kolkata: For airborne contamination you can put a control slice exposed to air. What do you think @Aditiya Joshi CUBE @Navaneel is the importance of controls in an experiment
[12:02 PM, 8/31/2021] Arunan HBSCE: Please post your Control simultaneously. @Batul Pipewala Kolkata
What were the Controls?
Are they not crucial?@Batul Pipewala Kolkata @Navaneel
Let’s go ste by step.
[12:03 PM, 8/31/2021] Arunan HBSCE: Let’s pay more attention to Design of experiment based on what is our Hypothesis that we are testing. @Batul Pipewala Kolkata @Navaneel
Let’s state the Hypothesis.
Yes, my control is on the side of the expt potato. It has every single exposure except to tap water. Any bacterial growth on that potato would be independt of tap water
[12:03 PM, 8/31/2021] Batul Pipewala Kolkata: Yes Sir controls will give a clear pic by comparison with the test
[12:04 PM, 8/31/2021] Batul Pipewala Kolkata: Can you show in the pic label the control
Just one potato slice or in at least duplicate?
Let’s have a full DESIGN, stated clearly. @Navaneel @Batul Pipewala Kolkata @Aditiya Joshi CUBE
Atleast 3 potato slices as test replicates would be needed besides the controls @Navaneel
yes it is labelled mam. I am sendin the experiemnt design none the less

Mango tree started flowering!

Flowering Started
No sprouting
No Fruiting Yet

Location: Kovilpatti, Tamilnadu
CUBE Homelab Kovilpatti

Date : 24/08/21
Time : 7.21AM

Latitude: 9.1727° N,
Longitude:77.8715° E

Temperature :
Maximum : 26°C
Minimum: 35°C

Data Collector: Chitra Achari Trying to recieve more photos from Kovilpatti.
Also clarify which test slices for submerged and surface culture?

30th August 2021 - CUBE Chatshaala Discussion - White Boards

Discussed about model systems : Fruit Fly, Mosquito, Ants (Insects).
30th August 2021 : Discussion white board
Where can we find Fruit Flies?

How Fruit flies Look Like? Is it easy to distinguish from other insects?

What are the characteristics features that very well visible in fruit flies? Which part of the body well visible like… Wings, Eyes, Abdomen…? What is the actual size of this small flies?
What are similarities & Dissimilarities between Fruit flies and Mosquitoes?

Fruit Flies and Mosquitoes, both of them are belonging to;

Kingdom : Animalia

Phylum : Arthropoda (Because both of them have joined appendages)

Class : Insecta (Body divided in to 3 parts Head, Thorax abdomen. Have 3 pair of legs & 1 pair of antenna, also have compound eyes.)

Order : Diptera
Both have 2 wing (one pair of wing)

Coming in to Family level Fruit Files are belonging to Drosophildae, Mosquitoes are belonging in to Culicidae.
One interesting point added by @Navaneel that insects have fore wing & hind wings. Only Fore wing is Expressed. Hind wing converted in to halteres in the case of Dipterans.

Flies are insects of the order Diptera, the name being derived from the Greek δι- di- “two”, and πτερόν pteron “wing”. Insects of this order use only a single pair of wings to fly, the hindwings having evolved into advanced mechanosensory organs known as halteres, which act as high-speed sensors of rotational movement and allow dipterans to perform advanced aerobatics. Fly - Wikipedia

Reference given by @Yash

But did other insects have halteres first?

Are there examples of insects having just halters…?

It is generally accepted that the halteres evolved from…
So if it is easy enough for you to keep an ant in the refrigerator and immobilize it have you obtained any immobilized ants yet?
Wow! Even before September?
Please get what percentage of Mango trees at this lower Latitude are flowering before September? @Abhijeet Singh
@Aswathy Suresh CUBE
intresting mam …
How do we really know what we got is Fruitfly and likely to be Drosophila? @Aswathy Suresh CUBE @Yash Sheregare CUBE @Raj Yashwantrao Patil CUBE @Aastha Ahuja Cube
Yes sir!
I trying to receive more data from this place and state! Crucial time in mapping these observations!

Home lab at Homi Bhabha Center

Quarantine Mode
Trapped three fruit flies in two bottles.

Seems to be of different types of fruit flies.
Can we standardise the angle at which we are taking the photos? Then maybe it can be analysed better. Angle as in the lateral view, or the ventral view.
Wow… Great what is your further plan @KIRAN CUBE ?
Plan is to start the single line Cultures of these fruit flies and identify them.

After getting 1st progeny of these fruit flies I will be transferring in medium.

Expecting to see eggs tomorrow (1st September 2021)

VIDEOS SHARED BY YASH AT 4.03PM

Updates on Fruit fly cultures
Location - Prabhadevi, Mumbai

SLPbA3
Highlight - On 4th day from transferring 3 fruit flies, there are no larvae seen today.

Media date - 26/08/2021
Transfer date - 27/08/2021
No of flies transferred on 27/08/2021 - 3

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality

31/08/2021
No of flies - 3
No of larvae - 0
No of pupae - 0
No of dead flies - 0

Expectations
31/08/2021 - more larvae and pupae - ~ 20
1/09/2021 - More pupae - 30
4/09/2021 Newly hatched fruit flies @Arunan HBSCE sir @Lydia cUBe Drosphila @Aswathy Suresh CUBE @Isha Pawle @Sidhy Kerala Drosophila @Janhvi Deshmukh
Updates on Fruit fly cultures
Location - Prabhadevi, Mumbai
SLPbB3
Highlights - SLPbB3 shows 5 pupae and a few larvae on 4th day of transfer of fruit flies

Media date* - 26/08/2021
Transfer date - 27/08/2021
No of flies transferred on 27/08/2021 - 30

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality

31/08/2021
No of flies - 28
No of larvae - 6- 3rd instar larvae
No of pupae - 5
No of dead flies - 2 @Arunan HBSCE sir @Aswathy Suresh CUBE @Isha Pawle @Sidhy Kerala Drosophila @Lydia cUBe Drosphila and others
Updates on Fruit fly cultures
Location - Prabhadevi, Mumbai
SLPbC3
Highlights - Only 2 larvae seen on 4th day of sub culture on 27/08/2021

Media date* - 26/08/2021
Transfer date - 27/08/2021
No of flies transferred on 27/08/2021 - 35

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality

31/08/2021
No of flies - 33
No of larvae - 2- 3mm larvae
No of pupae* - 0
No of dead flies - 2

Expectations
31/08/2021 - Pupae and larvae - 10-20
*1/09/2021 - More pupae - 20+
4/09/2021 - Newly hatched fruit flies @Arunan HBSCE sir @Aswathy Suresh CUBE @Isha Pawle @Lydia cUBe Drosphila @Sidhy Kerala Drosophila @Man Masih Beck RANCHI CUBE and others
Goof UP - Ignorance is not a Bliss - SLPbA2 culture flies shows a Different fly other than Fruit fly!. (suspect it to be a Phorid fly)
Media is liquidy!
I didn’t check this bottle for almost a week and couldn’t transfer the fruit flies that were present.
Today’s Headline - 24th day of Culture - SLPbA2 shows no fruit flies but a Different fly - suspect it to be Phorid fly!

Media date - 06/08/2021
Transfer date - 7/08/2021
No of flies transferred on 7/08/2021 - 35

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality

31/08/2021
No of flies - 1 (suspecting a Phorid fly)
No of larvae - 0,
No of pupae - 0
No of dead flies - 30

Immediate work done to save SLPbA line - 1) Made TRS-V media on 26/08/2021 and Transfer the Fruit flies 3 alive in New Media Bottle on 27/08/ 2021
2) Keep the SLPbA2 bottle separate so that it there is no chance of this fly contaminating other cultures
Today’s Headline - 24th day of Culture - SLPbB2 shows 20 fruit flies.

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality

Media date - 06/08/2021
Transfer date - 7/08/2021
No of flies transferred - 30

31/08/2021
No of flies - 20
No of larvae - 2- 3mm larvae
No of pupae - 0
No of dead flies - 20
[4:36 PM, 8/31/2021] +91 87289: Where was this bottle kept ?
[4:39 PM, 8/31/2021] +9187289: Are this kind of flies usually seen in the place … where you catch fruitflies?
What are these brown patches in the bottle? Are these pupae?

Can we also highlight the objective to map Drosophila melanogaster from different parts of the India that’s Geographical distribution of Drosophila melanogaster across the India by the CUBist.

Aswathy
Sidhy
Yash
Man Masih
Isha
(Please add names)

We are currently 5 people to have single line cultures of fruit flies and I have also initiated hopefully get 2st progeny, can we also take the initiative and find out which species are these?
Great! @KIRAN CUBE maam the second bottle on right shows an interesting fly trapped…
Almost looks like the bottom fly has black eyes
Are they fruit flies?
Let’s find out in somedays
Or make a guess
82 pupaes! That’s a huge number in a few days there would be plenty of fruit flies @Man Masih Beck RANCHI CUBE @Aswathy Suresh CUBE @Isha Pawle

CUBE Ranchi Re-Mans’ Home Lab
31-08-2021
Fruitfly Update

Immediate Objective: to start a single line fruitfly culture(RNSL-B) line at ReMan’s Lab on TRSV Media

Short Term Objective: -To identify the trapped fruitflies
-To find native Drosophila melanogaster species from Ranchi

Media preparation- 18-08-2021
Transferred date- 19-08-2021

OBSERVATION
after 12 days of transfer
•Larvae ~7
•Pupae ~82
•Fly ~ 12 to 15

Highlights
•flies first appeared on 28th Aug(9th day after transfer)
•most larvae have turned into pupae
[5:11 PM, 8/31/2021] Yash Sheregare CUBE: Today’s Headline - 24th day of Culture - SLPbC2 shows 10 fruit flies.

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality

Media date - 06/08/2021
Transfer date - 7/08/2021
No of flies transferred - 30

23/08/2021
No of flies - 10
No of larvae* - 0
No of pupae -
No of dead flies - 25
@Arunan HBSCE sir @Aswathy Suresh CUBE @Isha Pawle @Lydia cUBe Drosphila @
[5:11 PM, 8/31/2021] Yash Sheregare CUBE: These bottle is kept near doorway beside bed…

Goof up
I am guessing the cotton plug wasn’t tight enough and that invited a Different fly inside the Single line culture - a culture that was started with just 1 pregnat fruit fly that gives a pure culture
@Gaurangi Dhuri
The brownish and some what blackish patches are the empty pupal cases
Objective - To Culture and Map and find Drosophila melanogaster in our Locality and Entire India through Collaboration by CUBists
@Batul Pipewala Kolkata mam and @Aditiya Joshi CUBE I had a question. Are you using normal potato or boiled potato for the substrate?
For nematodes I am using raw potato @Aditiya Joshi CUBE is using boiled potato as a substrate for microbiological work
Ooh …

(upload://20zldg4skVENmrMPN7IKjIdbWmP.mp4)
Movement of fruit fly. Home lab HBCSE.
Has taken 12 days to pupate 82 larvae. Is it a huge number? @Aswathy Suresh CUBE @Sidhy Kerala Drosophila @Isha Pawle @Yash Sheregare CUBE @Rechel Turkey Cube Ranchi Moina and others…
![WhatsApp Video 2021-08-31 at 6.10.02 PM(1)|video]

KiranKalakotiR · September 1, 2021, 6:35am

31st August 2021, 9.30pm to 1st September 2021, 9.30pm
Has taken 12 days to pupate 82 larvae. Is it a huge number? @Aswathy Suresh CUBE @Sidhy Kerala Drosophila @Isha Pawle @Yash Sheregare CUBE @Rechel Turkey Cube Ranchi Moina and others…

Caught some mosquitoes at home(Ulwe-Navi mumbai)
Objective:- Continuing Prediction and prevention of mosquito born diseases(dengue, chickungunya) Project.
History of Mosquito project:-
I started mosquito project when I was in 7th grade(2015-16) with my school friends(Adarsha Vidyalaya, Tilak Nagar, Mumbai) and collaborators from Deonar public school(Govandi, Mumbai). Including @gaurangi. We together used to catch mosquitoes at school and individually at our homes. After we catch the mosquitoes we place them on a black background and look for mosquitoes with white stripes(easily visible on black background). The mosquito with white stripes are Aedes mosquito and they are responsible for spreading diseases like Aedes and Chickungunya. So we mainly look for these mosquito. Did this for around one year or two and found out that Aedes mosquitoes are found highest in August.

So now let’s see if we get any Aedes mosquitoes from starting of September in Ulwe, Navi-Mumbai.
Today (31st Aug 2021) I got 10 mosquitoes.
No Aedes mosquitoes.

Looking forward for collaboration. Aug 2021) I got 10 mosquitoes.
No Aedes mosquitoes.

Looking forward for collaboration and comments from @Gaurangi Dhuri @Hina Mudgal Kanpur CUBE @Aswathy Suresh CUBE @KIRAN CUBE @Arunan HBSCE @Batul Pipewala Kolkata
Aedes is not a disease… it’s a type of mosquito *
@Hina Mudgal Kanpur CUBE what’s the update on your mosquito studies!? Please share the updates on your work!
Yeah… I would love to collaborate with you… and restart our old project
@Abhijeet Singh @Gaurangi Dhuri
[11:12 PM, 8/31/2021] Abhijeet Singh: Sorry for that! It was a mistake!
[11:12 PM, 8/31/2021] Abhijeet Singh: Looking forward…!
No problem… I shall also restart with mosquito then…
Let’s plan accordingly! What should be our immediate objective @Gaurangi Dhuri !
Also short term and long term?
Let’s built it up based on our past experience. Help me recall too!!
That time we had two hypotheses… of the most no. Of mosquitoes all over the year… and avt aedes mosquitoes when found most…

VIDEOS SHARED BY ASWATHY AT 12.40AM
Fruitfly Update

BREAKING NEWS : 58 Days Old ‘E’ Culture Bottle Still Have Flies,Larvae,Pupae.

SLBK E15
Transfer Date : 02.07.2021
Transfer Flies : ~20
Gravid : ~8, Males : ~12

Objective : To find D.melanogaster from my native area (Bottukadavu - Thrissur Kerala) Want to compare with St. D.melanogaster.

31/08/2021
(observation today)

No of Flies : ~8
No of Larvae : ~9
No of Pupae : ~10
No of Dead Flies : ~50
Media Conditions : Slightly liquefied.

Update on : 31/08/2021
Location : Bottukadavu - Thrissur Kerala.
Microworms

43 Days Old Fruitfly Culture Bottles ; Microworms Started Presenting Inside Bottle Wall.

SLBK E16
Transfer Date : 19.07.2021

Objective : To Identify This Worm (Is it Nematodes or some other parasite.)

31/08/2021
(date of observation)

Very few number of microworms observed inside the culture bottle. Fruit flies, Larvae, Pupae also present inside the culture bottle. Media solidified conditions.

In order to identify microworms before need to culture them separately.

Immediate Objective : To make media (TRSV) to culture microworms separately to identify them.

Breaking News . Observed approx 20 Larvae in A bottle that’s post 24 hours of trapping fruit flies.
Observed single egg in the B bottle after 17 hours trapping of fruit fly. Date of photo: 1st September 2021, 8amA great beginning again @Abhijeet Singh after a long break during 10th Grade till 12th Grade.
But, don’t you think the lighting is too much that we cannot see the image as expected?
What should be the lighting conditions for getting a picture that can show clearly black and white stripes? @Abhijeet Singh @Gaurangi Dhuri @Hina Mudgal Kanpur CUBE @Aswathy Suresh CUBE
Caught 5 mosquitoes yesterday night at 11.45 pm 0 aedes found as tried identifying on black background…How to identify Aedes @Gaurangi Dhuri
The image is not clear to get evidence that none of them are Aedes mosquito @Abhijeet Singh
How do we resolve it?

What is the minimum quality of an image, for achieving the objective? @Abhijeet Singh @KIRAN CUBE @Saida Sayyed @Aswathy Suresh CUBE @Aditiya Joshi CUBE @Man Masih Beck RANCHI CUBE and others.
If the body of the mosquitoes have white stripes present on it including the legs region… then it’s an aedes mosquito
Great! @Gaurangi Dhuri
Which college are you presently in? Let’s start a CUBE group with your friends and their relatives for CUBE HomeLabs. @Gaurangi Dhuri
[9:07 AM, 9/1/2021] +91 88988 87289: Maybe the sorrounding high power of light from the top should be avoided…
[9:08 AM, 9/1/2021] +91 88988 87289: I am in V.G.Kelkar college… yes sir… I have thought of introducing CUBE to our college’s research grp
From white stripes we confirm its a aedes ? @Gaurangi Dhuri
Yess
Any another features ? @Gaurangi Dhuri
Please highlight the mistake @Abhijeet Singh Let’s celebrate the Goof-ups, big and small. Let’s have Confessions of Scientists!
There are other characteristics like… the sitting position and all… but for these characteristics to be used for identification… we need to keep observing the mosquitoes when they are alive … But we can easily see the white stripes with our naked eyes when we kill the mosquito
[9:16 AM, 9/1/2021] +928389: In continuation to this
[9:19 AM, 9/1/2021] +928389: Breaking new from Navaneel’s home lab on the analysis of tap water samples.

Before breaking the seal, this is the 24 hour observation of te experimental potato substrate streaked with tap water sample. There are no apparent contamination, or growth of bacterial colony isolates on the surface of the potato sample.
This is the 24 hour observation of the control sample. It correlates that there was no contamination of the sample there are no apparent growth of bacterial colony isolates on the surface of the potato.
This is 24 hour observation of submerged culture of tap water in coconut water. Due to the natural milkiness of coconut water, it is difficult to say if there is any bacterial growth. However, I made a mistake of not keeping a control in this case which makes differentiating difficult. This needs to be noted I exp design.[9:29 AM, 9/1/2021] +91 98308 28389: Possible Avenue of reason for no growth of colonies on expt potato ;

There is no bacterial contamination in tap water
The contamination is an obligate aerobe, an since I sealed the petri dish, it couldnt grow optimally. Due to this, I have to envision some control to remove this conclusion.
The contamination in water sample is a slow grower and does not culture in 24 hours
The contamination does not find adequate growth medium in potato based substrate.
5 @Aditiya Joshi CUBE used boiled potatoes, which degrades some complex material. I used raw potato. This may have lead to difficulty in the bacterial growth.
[9:31 AM, 9/1/2021] +91
28389: Next step of experiment: to debug all of the above problems, so that only 1 is the reasonable answer. I am hopeful of collaboration in that aspect.

To debug problem 5, I will use tap water and culture it on boiled potatoes and seal it.

To debug problem 3, I am going to leave my present potato samples for 24 hours more.

To debug this step, I am going to use tap water to culture on both raw and boiled potato but without sealing off the petri dish
[9:32 AM, 9/1/2021] +91 928389: In case of number 4, I do not have an answer yet
[9:32 AM, 9/1/2021] +928389: Due to the good up in the submerged culture, I am going to redo the step once more with fresh medium and control.
[9:33 AM, 9/1/2021] +91 28389: Te present culture, just for the sake of it, I am going to plate it on potato surface medium
[9:38 AM, 9/1/2021] +91 28389: Objective : analysis of tap water samples
Date : 1/09/21
Data collector : Navaneel Sarangi

image900×1600 91.5 KB

Objective : to study bacterial growth from coconut water medium after 24 hour culturing period on boiled potato medium with aerobic respiration
Date 1/09/21
Image : Navaneel Sarangi
Location Saltlake kolkata
image900×1600 82.9 KB

Control slice
Is “Rava” a source of nutrient for Microbes?

Is the discussion about developing an alternative Homemade Nutrient Broth like the way Fruitfly Collaborators developed an alternative of Cornmeal Media? @Aditiya Joshi CUBE @Navaneel and others…
[10:00 AM, 9/1/2021] +91 28389: Yes.
[10:00 AM, 9/1/2021] +91

Breaking news… I got 10 mosquitoes in just around 5-10 mins in the bathroom… at 10am when I was taking a bath… But yesterday night when I checked in the bathroom, I was unable to find a single mosquito…But I made a goof-up that… Many of the mosquito body parts got damaged … I think that happened as I caught the mosquitoes with wet hand and kept them when on a damp place due to which while picking them … many parts of mosquitoes break apart.
Please post some photos @Gaurangi Dhuri @Janhvi Deshmukh
Better lighting will help.@Navaneel
Yes sir, I also think that😅.
I think the problem is not lighting but focus of my camera. I’ll try to clear image next time!

What do you think @Gaurangi Dhuri @Aswathy Suresh CUBE ?
I think it’s not just focus of camera… but the background light from the top have affected the photo … due to which we are unable to see the mosquitoes clearly as in the white stripes
Yes sir I got your point!
So as we are looking for Aedes mosquito which has white stripes on its legs we need to have a clear photo to show the evidence to everyone that yes none of them is Aedes.

I think the minimum quality of an image should be that the should be clear in the sense of what are we trying to show through it!

So as we want yo show that none of the mosquito are Aedes(white striped once) the Focusing and all of the camera should done as per that!

Please correct me if I’m wrong @Arunan HBSCE and others!
Don’t you see some whitish patches?
How about giving essential information like Objective, Date, Location of HomeLab, Name of Data collector with every photo for readers to get the Context. @Navaneel

I think this image would help us to understand that how Aedes mosquito looks like! So we can clearly see the white stripes on the legs and body of the mosquito . By which we identify that yes it’s a Aedes mosquito. @Janhvi Deshmukh @Gaurangi Dhuri
Please give essential information like Objective, Date, Location of HomeLab, Name of Data collector with every photo for readers to get the Context. @Navaneel
Reference :- Wikipedia

Yeahh … this can surely help us to know… how exactly the aedes mosquitoes look like
What is your Hypothesis? @Navaneel Please state the Hypothesis.

These are some of my aedes mosquitoes … this is an old photo … In this the white stripes are visible… If u check closely
Any explanation why the potato slice is already bot looking intact? @Navaneel
What should be the ideal design to test your Hypothesis? @Navaneel
Sir the boiled potato is inherently very unstable sir

. That lead to problems in generating proper slices.

I am writing the entire expt in words, so that is is easier to read and in one place. I will send it in by today.
Please describe in full, short sentences @Navaneel so that a newcomer too can follow it.
The four mosquitoes which are at the bottom we can clearly see white stripes… and even in the top one… but in this picture also… due to some light issues… all the mosquitoes are not clearly visible to check white stripes
How about posting better photos showing lighting, focus and contrast.
How to improve the photography? @Gaurangi Dhuri
Yes sir … we need to sort out with the photography … so that we can clearly check whether the white stripes are present from photos
Yes sir!!!
So actually in earlier post I did a typing mistake that:-
Aedes mosquito are responsible for spreading diseases like ’ Aedes ’ and Chickungunya.

So the the words Aedes that I have strikedthrough was a mistake and it was conveying that Aedes is a kind of Disease.

Correction:-
Aedes mosquito are responsible for spreading diseases like Dengue, Chickungunya.
[10:31 AM, 9/1/2021] Arunan HBSCE: Which parts of mosquitoes are important for achieving your Objective?
Please always start with Objective and also give Location of CUBE HomeLab, Date, Name of Data Collector with each photo @Gaurangi Dhuri
[10:32 AM, 9/1/2021] Arunan HBSCE: What is your comment on the bathroom mosquito catch by @Gaurangi Dhuri ? @Abhijeet Singh
I think … I saw many mosquitoes in the bathroom today… because in the night they maybe coming out in other rooms for food and in morning they maybe coming to their original position… maybe they are breeding in bathroom itself…
[10:35 AM, 9/1/2021] Arunan HBSCE: So what is your plan to trouble-shoot this issue of taking photos? @Abhijeet Singh
Please comment on photos of @Navaneel
Let’s improve the quality of data while posting.
Let’s have a discussion to resolve this urgently and publish a Blog on it in Stem Games.
[10:37 AM, 9/1/2021] Arunan HBSCE: What is our Long term Objective by collecting Mosquito data? @Gaurangi Dhuri @Abhijeet Singh @Hina Mudgal Kanpur CUBE
Are they all fruitfly larvae?
[10:40 AM, 9/1/2021] +91 87289: Okk … so our basic objective to catch mosquitoes is to find in which month in the whole year are we getting more mosquitoes and in which month are we getting more aedes mosquitoes… so to identify aedes mosquitoes… we need to find whether the legs and stomach of mosquitoes are having white stripes… If there are white stripes present in the legs then it’s an aedes mosquito… so to identify this… we need to take care that … the legs are not damaged
[10:43 AM, 9/1/2021] +9187289: Our long term objective is to find the most amount of mosquitoes in which seasons… and most no. Of mosquitoes in which season… so accordingly we can take more precautions in particular season… and even inform others
[10:43 AM, 9/1/2021] +91 87289: Am I right @Abhijeet Singh @Hina Mudgal Kanpur CUBE … ??
What are the white spots in the pic are they not bacterial colonies @Navaneel
[10:47 AM, 9/1/2021] +9128389: Mam i think they are instrinsic to the potato
[10:47 AM, 9/1/2021] +91 8389: those are starchy exudates which I was observing. Quite distinct from bacterial mucilaginous colonies.
[10:48 AM, 9/1/2021] +9128389: But I can be wrong in identification, I will break isolate tomorrow to allow for debuggin the long term growth problems28389: for plating in general

Are u talking About this white spots @Navaneel … ??Did you see them before plating? Any pic evidence?
[10:48 AM, 9/1/2021] Batul Pipewala Kolkata: Yed
[10:49 AM, 9/1/2021] Batul Pipewala Kolkata: Yes
ah no. those are water vapour marks
[10:49 AM, 9/1/2021] +91 87289: Yaa that’s what I thought
[10:49 AM, 9/1/2021] +91 87289: Hence asked
I will click a better picture
Okk
Ok

[10:50 AM, 9/1/2021] +91 28389: This was the start of the experiment
[10:50 AM, 9/1/2021] +9128389: I should have clicked a closer picture
What was your expectations in each case @Navaneel !?
I think with the objective, expectations are also an important aspect!!
[11:37 AM, 9/1/2021] +91 28389: .
[11:37 AM, 9/1/2021] +91 28389: @Abhijeet Singh
[11:45 AM, 9/1/2021] Abhijeet Singh: I think you mean to say ‘goof up’.
So as you are going to redo the submerged culture, what will do do with the submerged culture you have currently!?
I think we should also follow up that! Let’s see what happens!!
That’s would be great @Navaneel !! Please send us updates!
Also when you redo the submerged experiment let’s think of what are you going to name it! Because the labels of the new one should not let us forget the one which you have currently let’s together follow it up!
@Navaneel @Aditiya Joshi CUBE @Arunan HBSCE @Aswathy Suresh CUBE
[11:51 AM, 9/1/2021] Abhijeet Singh: I think the legs are very important for differentiating between Aedes and non-aedes mosquitoes. So while trapping lets make sure that we don’t hit that hard that it’s legs gets broken from its body!
What do you think @Gaurangi Dhuri !?
Yess… I also wrote the same thing… that legs plays an important role in indentification
The photos which she sent needs to have a better resolution!
And gaurangi and I discussed about catching mosquitoes in bathroom. So actually at my place (ulwe) mosquitoes come in evening by 5.45 pm or something like that.
At day time we can see that. When we do cleaning and all in the morning they are found behind the table or underneath the bed. As in the dark places!
And better lighting and better focus too.
Please start clicking and posting better evidences. @Navaneel
yes sir
Now I’m not able to make it in a statement but let’s try to construct it through what we understand.

So what I think our long term object should be is that we should be to able to predict the diseases that may occur in a month or months particularly as per the data we collect through mosquitoes Trapping and identification of mosquitoes trapped

I don’t know if the idea is transferred but let’s discuss!!
@Gaurangi Dhuri @Arunan HBSCE @Hina Mudgal Kanpur CUBE
What do you mean by submerged experiment?! @Abhijeet Singh @Navaneel
What is the objective?
What is the research question?
Yes, what is your expectation based on your stated Hypothesis? @Navaneel
Design of the experiment :

an earbud soaked in tap water is immersed into 5ml of Coconut water. The experiment is being carried out in a safe enclosed environment, adjacent to a doctor’s chamber.

Expectations : There should be no microbes present in the tap water submerged culture as it is chlorianted by the Municipality. Likewise, the surface culture on potato should not yeild any bacterial isolates.

Alternate Hypothesis : If there is presence of colonies of microbes on submerged or surface culture, there is contamination of microbes in the tap water being used for drinking resources.

Since most of us use this water in one way or the other without additional filtering, and there have been no untoward incident so far, I expect the microbes been enrichened are not pathogenic.
How about looking around for possible breeding places too? @Gaurangi Dhuri @Abhijeet Singh @Navaneel
Can Pagalapos be sites for mosquito breeding? @Abhijeet Singh @Gaurangi Dhuri Can’t we restart Pagalapos as model for many research studies? @Saida Sayyed @KIRAN CUBE @Shivam CUBE NEMATODE
Submerged culture : The bacterial growth happens in liquid media (Coconut Water)

Surface Culture : The bacteria growth happens on the surface of a solid medium (potato)
What is meant by resolution? @Abhijeet Singh @Navaneel @Gaurangi Dhuri @Aastha Ahuja Cube @Ishita Assam
[12:07 PM, 9/1/2021] +987289: Yess… we can predict the disease caused by mosquitoes especially dengue and chickenguniya as its caused by aedes mosquitoes… in which season or which particular month… and if we get collaboraters then we can find whether the location affects the causing of disease in particular seasons or months … @Abhijeet Singh right?
[12:08 PM, 9/1/2021] +917289: Yess… It can be great work… if we connect pagalapos and mosquitoes studies…
So what @Navaneel and I mean from ‘Submerged experiment’ is that
In one of the cases navaneel is using Coconut water as a source for growing microbes(if present) in tap water.

So @Navaneel used an ear bud and dipped in tap water and kept that in a container with coconut water. So that ear bud containing tap water sample is ‘submerged’ in coconut water.

I think I’m getting it correct @Navaneel . Please correct me if I’m wrong anywhere!
If I could please know what are pagalapos ?
You mean you want to predict Mosquito borne diseases in the area through this? Please clarify.
What is the incidence of Mosquito borne diseases in your locality, as per Department of Health?
[12:09 PM, 9/1/2021] +91 28389: yes
[12:09 PM, 9/1/2021] +91 28389: absolutely
[12:11 PM, 9/1/2021] Arunan HBSCE: Fresh coconut water? Because there will be possible differences in the microbe profile of fresh coconut water vs that was kept for sometime. Don’t you think so? What is your explanation on this? @Navaneel @Abhijeet Singh @Gaurangi Dhuri @Aditiya Joshi CUBE
[12:13 PM, 9/1/2021] Arunan HBSCE: Is the last sentence dealing with your Hypothesis? Can you state the Hypothesis more clearly as in Science, so that it can be verified or falsified? @Navaneel
[12:15 PM, 9/1/2021] +91 28389: Yes sir. Using fresh coconut water. But I want to do the exp with the remnant water as well, however I have not designed the exp yet.
[12:15 PM, 9/1/2021] +91 28389: yes sir
Please describe step by step procedure, how you prepare this set up of submerged culture? @Navaneel
Objective : Study of microbial contamination in Tap Water in Saltlake, Kolkata

Hypothesis : Due to proper Chlorination of water by the Municipality, I hypothesis that there are no microbes to be cultured from the Municipal water source.
Can’t we do this study of incidence of Mosquito borne diseases, across the country? @Gaurangi Dhuri @Abhijeet Singh @NavaneelStep 1 : Clean the coconut surface. Spray 70% alcohol to clean the cocnut outer shell. Ensure hands are washed to prevent contamination

Step 2 : Use a sterilised Rock / Sickle to break the coconut. Collect water with minimum contamination with cocnut fibres in Sterile collected

Step 3: use boiled muslin cloth (for sterilisation) to seperate fibres from the coconut water.

Step 4: (Variations from this step) → Take 5ml aliqout from cocnut water source.

Step 5 : Collect 10 ml tap water in sterilised collected and soak sterilised ear bud in tap water.

Step 6 : Dip Ear bud into 5ml coconut water aliquot in sterile collected.

Step 7: (Variations) → Close cap and seal with parafilm to prevent airborn contaminations.

Step 8 : Make 5ml cocnut water control in sample collecter. Exact steps as listed above are followed except dipping ear bud into the medium. The cap is closed and parafilmed.

Variations for parafilm : Dissolvable glue. Vaseline. Wax.

Variation in steps → Parafilm should not be used if the culture is aerobic. It may generate anaerobic conditions in brothHow will u proceed with experiment?
Is there any specific reason behind dipping the bud in tap water n then putting it in coconut water @Navaneel
[12:34 PM, 9/1/2021] +91 28389: Tap water is the inoculum.
[12:34 PM, 9/1/2021] +91 8389: Coconut water itself doesnt have any microbe in it for fermentation
[12:34 PM, 9/1/2021] +91 28389: it myst be exposed to air or inoculum
Okay… But how dipping the bud will be needed. Can we simply add a drop of tap water or 1ml of tap water as such directly in coconut water
i think the bud provides an additional substratum, in case the microbes are very les sin number. it is strictly not needed, but we used to do it in our lab when there was really less concentration of a culture. And also the bud provides a mesh to capture the microbes form the tap water, instead of the entire water entering into the media and diluting it, along with chlorine ions.
[2:24 PM, 9/1/2021] +91 88988 87289: Yess we can… we have to contact with BMC offices and collect the data of different wards and stated all over the country… and then we can compare it with our studies … and check whether our observations are going right or there is something which is missing… what do u think
@Abhijeet Singh @Hina Mudgal Kanpur CUBE @Arunan HBSCE ??
[2:28 PM, 9/1/2021] +91 88988 87289: Pagalapos are basically the holes that are present on the lid of the gutters… in the rainy season this holes will have water and other times it can be dry… these holes may have different organisms… there are even possibilities that pagalapos are the breeding site for mosquitoes @Navaneel …
Am I right @Abhijeet Singh @Arunan HBSCE @Hina Mudgal Kanpur CUBE … if I am wrong … pls correct me…
Please post some photos of Pagalapos sites from your locality @Gaurangi Dhuri @Abhijeet Singh

Breaking news. …pupae stop developing… I trapped flies on 8.30 pm on 29/07/2021 …after that I seen some larve on 1/08/2021 … it’s a open Bait setup in which I used tomato and cucumber as a bait…after that I seen some big larve on 4/08/2021 it’s a housefly larve …in that setup I seen two types of larve some are 3 mm in size and some are 5 mm in size I seen some pupue on 05/07/2021 some are in 3 mm size some are 5 mm in size after that I waiting for new flies some housefly emerge and one fruitfly emerged on 08/08/2021 but there so many pupae you can seen here they stopped developing I couldn’t seen from 15 days when I seen today I found to that pupae are not developing …

. And why ? Location: Wardha data collector: janhvi deshmukh. Time :5.07 pm janhvi deshmukh homelab bajaj College of science wardha Maharashtra
Fruitfly and housefly pupae… Data collector: janhvi deshmukh location :wardha
Some fruitfly pupae…on tomato and some dead housefly … location:wardha. Data collector: janhvi deshmukh@Arunan HBSCE sir @Aswathy Suresh CUBE @Aditiya Joshi CUBE @KIRAN CUBE @Yash Sheregare CUBE @Batul Pipewala Kolkata mam and others…Biologists Just Got Closer to The DNA Secrets That Stop Species From Interbreeding : ScienceAlert

This is fascinating
Something that maybe cubists can think of testing in their fly cross breeding of strains from different parts of the country, to see if they aren’t compatible due to sattelite DNA

KiranKalakotiR · September 3, 2021, 8:20am

1st September 2021, 9.30pm to 2nd September 2021, 9.30pm
VIDEOS SHARED BY ASWATHY AT 11.27PM
Fruitfly Update

21 Days old culture bottle have ~10 Larvae, 8 Pupae, 1 Fly.

SLBK A22
Transfer Date : 12.08.2021
Transfer Flies : 1 Fly

Objective : To Find Drosophila Melanogaster from Native area.

01/09/2021
(observation today)

No of Flies : 1
No of Larvae : 10
No of Pupae : 8
No of Dead Flies : 1
Media Condition : Solidified

Update : 01/09/2021
Location : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
Fruitfly Update

21 Days old culture bottle have ~20 Larvae, 70 Pupae, 20 Flies.

SLBK B21
Transfer Date : 12.08.2021
Transfer Flies : 13 Flies

Objective : To Find Drosophila Melanogaster from Native area.

01/09/2021
(observation today)

No of Flies : ~20
No of Larvae : ~20
No of Pupae : ~70
No of Dead Flies : ~20
Media Condition : Solidified

Update : 01/09/2021
Location : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
Fruitfly Update

21 Days old culture bottle have ~20 Larvae, ~90 Pupae, ~60 Flies.

SLBK E17
Transfer Date : 12.08.2021
Transfer Flies : 14 Flies
Gravid : 6 ; Male : 8

Objective : To Find Drosophila Melanogaster from Native area.

01/09/2021
(observation today)

No of Flies : ~60
No of Larvae : ~20
No of Pupae : ~90
No of Dead Flies : ~30
Media Condition : Solidified

Update : 01/09/2021
Location : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
objective:- Prediction and prevention of mosquito born diseases Today(1st September 2021) I got 3 mosquitoes. None of them are Aedes! Observations noted in Log book and this is the photo of mosquitoes on a black background!
What is happening with E15, E16!? I think it’s important to follow them up also!
What do you think on this @Aswathy Suresh CUBE !?

VIDEOS SHARED BY SIDHY AT 12.00AM
SLTT-H
Single line culture of fruit flies.
Started on 16/08/2021, 11:40 AM
TRSV medium - 15/08/2021, 11:00 PM

Observation on 31/08/2021( 15 th day )
~ 14 larvae
2 pupae
~25 flies.

Location : Thanniam, Thrissur, Kerala
Sidhy.P.P, CUBE S N College , Nattika.
SLTT-I
Single line culture of fruit flies.
Started on 16/08/2021, 11:40 AM
TRSV medium - 15/08/2021, 11:00 PM

Observation on 31/08/2021(15 th day )
~18 larvae
7 pupae
~15 flies

Location : Thanniam, Thrissur, Kerala
Sidhy.P.P, CUBE S N College , Nattika.
SLTT-J

15 th day : No larva, No pupa , Less no. of flies , Fungal growth

Single line culture of fruit flies.
Started on 16/08/2021, 11:40 AM
TRSV medium - 15/08/2021,11:00 PM

Observation on 31/08/2021 (15 th day )
0 larva , 0 pupa , 4 flies
Fungal growth in the medium

Location : Thanniam, Thrissur, Kerala
Sidhy.P.P, CUBE S N College , Nattika.
Surely @Abhijeet Singh . SLBK E15 (58Days), E16 (43Days) are quite old previous subculture bottles of ‘E’ line. But its still have Flies, Larvae, Pupae…

SLBK E15 (58 Days) subculture bottle have ~7 Flies, ~5 Larvae, ~10 Unhatched Pupae.

SLBK E16 (43 Days) subculture bottle have ~15 Flies, ~10 Larvae, ~20 Unhatched Pupae.

(video will share tomorrow)
What is your further plan @Sidhy Kerala Drosophila?
summary

Fruitfly Culture
Location : Bottukadavu - Thrissur Kerala.

Objective : To Find Drosophila melanogaster from Native area.

Currently have 3 Single Line Culture ;

SLBK A, SLBK B, SLBK E

Maintain flies cultures by subculturing; Current subcultures are…

A Line - SLBK A22
B Line - SLBK B21
E Line - SLBK E15, SLBK E16, SLBK E17.

Description about each subculture bottles;
Current culture bottles which have flies, larvae, pupae

SLBK A22 (21 Days)
Transfer date : 12.08.2021
Transfer no flies : 1

(01/09/2021 - Observation)
No of Flies : 1
No of Larvae : 10
No of Pupae : 8
No of Dead Flies : 1
Media Conditions : Solidified

SLBK B21 (21 Days)
Transfer Date : 12.08.2021
Transfer no flies : 12
Gravid : 6 ; Male : 6

(01/09/2021 - Observation)
No of Flies : ~20
No of Larvae : ~20
No of Pupae : ~70
No of Dead Flies : ~20
Media Conditions : Solidified

SLBK E17 (21 Days)
Transfer Date : 12.08.2021
Transfer no flies : 14
Gravid : 6 ; Male : 8

(01/09/2021 - Observation)
No of Flies : ~60
No of Larvae : ~20
No of Pupae : ~90
No of Dead Flies : ~30
Media Conditions : Solidified

SLBK E16 (43 Days)
Transfer Date : 19.07.2021
Transfer no flies : 30
Gravid : 9 ; Male : 21

(01/09/2021 - Observation)
No of Flies : ~15
No of Larvae : ~10
No of Pupae : ~20
No of Dead Flies : ~40
Media Conditions : Solidified

SLBK E15 (58 Days)
Transfer Date : 02.07.2021
Transfer no flies : 15
Gravid : 8 ; Male : 7

(01/09/2021 - Observation)
No of Flies : ~7
No of Larvae : ~5
No of Pupae : ~10
No of Dead Flies : ~50
Media Conditions : Liquefied

Collaborators : @Arunan HBSCE sir @Yash Sheregare CUBE @Man Masih Beck RANCHI CUBE @Sidhy Kerala Drosophila @Rechel Turkey Cube Ranchi Moina @KIRAN CUBE ma’am @Lydia cUBe Drosphila ma’am @Aastha Ahuja Cube @Isha Pawle
Today 02/09/2021, there are only 2 flies in the bottle SLTT-J. Further plan is to transfer the flies to new TRSV medium.
[7:34 AM, 9/2/2021] Arunan HBSCE: So, you have a healthy SLBK A bottle culture with 10 Larvae and 8 Pupae. Are you sure? If so, that’s great news @Aswathy Suresh CUBE
Please write the summary of this. @Aswathy Suresh CUBE
[7:38 AM, 9/2/2021] Arunan HBSCE: Please post CUBE Bottukadavu HomeLab News in just three attractive lines.
To me, it looks like, @Aswathy Suresh CUBE has healthy A,B & E fruitfly Single Line cultures.
Please confirm @Aswathy Suresh CUBE
[9:25 AM, 9/2/2021] +91 8389: Breaking news from Navaneel’s home lab
[9:29 AM, 9/2/2021] +91 28389: Experiment 3 : bacterial culture of submerged culture specimen of tap water from saltlake kolkata

Continuation of images from 1/09/21.

In Exp 2, we had taken a ear bud full of tap water and inoculated coconut water medium. We had allowed incubation for 24 hours. Following the, we had streaked the sample on potatoes from expt 3 (as stated above). The potatoes are meant to act as a solid substrate for microbial growth. The potatoes were boiled, and the petri dishes were not sealed, but covered to allow aerobic respiration.

A control with no streaking was maintained as well

Pic A : Objective - to study bacterial contamination in tap water of saltlake kolkata. Date : 2/09/21 picture by : Navaneel Sarangi Location : Saltlake Kolkata.
In this picture we can observe a white mucilaginous coating growing on te surface of the potato. This picture is taken for reference before breaking sterility
contamination in tap water from saltlake, Kolkata. Date : 2/09/21 picture taken by : Navaneel Sarangi location : saltlake kolkata
In this picture we an seen clear mucilaginous covering and growth on the surface of the potato. I comparison this was the picture yesterday

We can trace vaguely the path of microbial growth along the potato. This also verifies that the bacterial growth was from the coconut water preferentially.
Pic c : This is an image of control. Objective : to study bacterial contamination of tap water in saltlake Kolkata date: 2/09/21 location : saltlake Kolkata picture by : navaneel Sarangi
We can observe no mucilginous growth. However there is certain red splotches. This I am not able to explain.
[9:43 AM, 9/2/2021] +91 98308 28389: Closer image of control
[9:45 AM, 9/2/2021] +91 98308 28389: The reason for growth can be : utility of a prior submerged culture step
2. Used of boiled potato
3. Use of aerobic conditions.

We will have to ascertain which ones of these are trueWhat is your immediate plan of work? @Sidhy Kerala Drosophila
[10:10 AM, 9/2/2021] Arunan HBSCE: Excellent! @Sidhy Kerala Drosophila
SLTT H fly culture is doing good on 15th day in TRSV medium.

Please write a summary of your SLTT cultures in not more than two sentences for capturing attention of readers and for potential collaborators. @Sidhy Kerala Drosophila
[10:17 AM, 9/2/2021] Arunan HBSCE: Can we assume that you have 3 different healthy SingleLine cultures from Bottukadavu locality?

If so, what is your immediate Plan of Work, to save them based on your earlier experience? @Aswathy Suresh CUBE

Don’t you think you have to have some urgent Immediate Plans? @Aswathy Suresh CUBE
[10:19 AM, 9/2/2021] Arunan HBSCE: @Sidhy Kerala Drosophila Please give your detail plan with date and time if preparing medium onward @Sidhy Kerala Drosophila
[10:20 AM, 9/2/2021] Arunan HBSCE: Please always give Location of the CUBE HomeLab and Date. @Navaneel

Very amused to see the potato is not sliced with a sharp knife with edges ‘eaten up’ @Navaneel Can’t we be more keen to *get a smooth slice like the smooth surface of the culture medium, in the petri dish? @Navaneel
yes sir I am trying that. With the boiled potato, it becomes highly unstable to get a perfect cut. Maybe a half boiled potato will be better stable to get a more smooth cut
Please give dates and after how many days etc., so that readers will find to follow you easily and join you as collaborators. @Navaneel
yes sir I mentioned here. I am sorry, I should have mentioned that the potatoes were streaked 24 hours earlier
Is this inoculated with coconut water?
What happened to your raw potato slices streaked day before yesterday?@Navaneel
[10:31 AM, 9/2/2021] Arunan HBSCE: When was this set up prepared? @Navaneel

What was your expectation and what is your observed result? Any comments? @Navaneel

And when was the photo taken, as date of observation? @Navaneel
[10:33 AM, 9/2/2021] Arunan HBSCE: Do we have any Controls? Don’t you think appropriate Controls are important for each experiment?
How will we redesign the experiment to convince all of our result? @Navaneel
What exactly is meant by ‘submerged’?
Please clarify @Navaneel
[10:38 AM, 9/2/2021] +91 98308 28389: As mentioned yesterday sir by @Abhijeet Singh and me, culture is a the growth of microbes in a favoured environment. There are various kinds of culture method, one of which is submerged culture. in this case the microbe is given a liquid medium of growth, so that the colony of microbe is submerged for growth inside the liquid.
[10:39 AM, 9/2/2021] +91 98308 28389: Sir I made 1 control, to compare between tap water exposed potato and no top water exposed potato. Ideally, we shoudl also have controls for boiled and raw potato, aerobic and anaerobic cultures as well.
[10:46 AM, 9/2/2021] Arunan HBSCE: How can we get potato slices reasonably similar to the petridish culture medium, instead of looking uneven and shattered?

How is boiled potato a simulation of petridish medium in conventional lab? This has been an important comparison while preparing alternate HomeLab medium for Fruitflies too.
So, let’s justify why slices of boiled potato can be simulation of lab medium for culturing microbes? @Navaneel @Abhijeet Singh @Aditiya Joshi CUBE and others
[10:48 AM, 9/2/2021] Arunan HBSCE: Please describe the method of preparing boiled potatoes from the beginning. @Navaneel for all to follow.

Please post short clippings of each stage of preparing the potato slice. @Navaneel
Hi @Navaneel, interesting experiment. I had a few questions about experiment design:

What is your control, can you explain?
How much sample have you added to your potatoes?

Can we do something to ensure that the growth is not from the coconut water itself?
[10:50 AM, 9/2/2021] Arunan HBSCE: Please photos of the whole sequence starting from freshly prepared potato slice till the latest observation with essential description and your conclusion, so far. @Navaneel
[10:55 AM, 9/2/2021] Arunan HBSCE: Are you having a liquid culture imitating nutrient broth culture?
Or you are using Potato to try simulating Nutrient Agar medium?
Please clarify before using complicated terms like submerged culture.
In case you have some reasons to use the term submerged culture please describe so that all can follow you and join you as collaborators. @Navaneel @Abhijeet Singh
[10:56 AM, 9/2/2021] Arunan HBSCE: Too much information in one sentence! Please clarify. @Navaneel
Yes sir, we are trying to develop a liquid culture to come as close as possible to Nutrient Broth. Potato is trying to simulate Nutrient Agar Medium sir.

For this, @Aditiya Joshi CUBE and I are trying to come up with a more standardised medium for bacterial growth using coconut water and some gelling agent like Rava.
Please finalize your solid medium simulation using boiled potato slices since you faced with several problems in your attempt.
Let’s resolve that first, since colony characteristics serves as important beginning and solid medium like boiled potato slices should help us, to start with.

What do you think needs to improved based on your initial attempt? @Navaneel
Let’s resolve this, first.
What is your comment? @Navaneel
@Aditiya Joshi CUBE please add on

Standardisation in potato medium :

How are we going to sterilise the medium ? Is boiling the entire potato enough ?
How can we create a plane surface with a soft boiled potato ?
Due to the inherent yellow colour of the potato, the colonies formed are often not visible. Is there any method to solve this issue ?
We usually overturn a petridish when colonies are growing to prevent condensed water varpour from falling on the medium. Since the potato is not fixed, the plate cannot be overturned.
Please describe step by step, how did you do it last? @Navaneel
And what improvement you would like to make this time, based on what you

VIDEO SHARED BY ISHA 11.31AM
2 Sep 2021
11:20 am
Worli, Mumbai
Fruitflies on lemon Slice
Process of making Potato-based Simulant of Solid Substrate (Prospective Nutrient Agar)

Step 1 : Wash Potatoes with soap and water to remove dust and contaminants. Soak in soap water to lyse most bacterial cells for 10 mins. (Not standardised : arbitary time taken - Need test for this)

Step 2 : Boild uncut potatoes (Standardisation required to extent of boiling. Essential for proper cut later on in experiment)

Step 3 : 70% ethanol wash of Potato for final sterilisation of cooled boiled potatoes

Step 4: Use sterile scalpel to careful make regular thin cuts with no potato peel in the medium.

Step 5 : Place potato in sterile petridish

Step 6 : Streak the surface of potato evenly.

Boiled Potato Slices replace to Nutrient Agar, at CUBE Sophia HomeLab, South Mumbai, Before transferring microbes from fermented coconut water, July 20, 2021 From CUBE Archives mcarunan
Modifications :
Need hard boiled potatoes. This will require modifications in the boiling steps.
Will need to pay closer attention to cutting the slices

Alternative :
Smash soft boiled potatoes and mash them till they are a hard bolus. Use this to fill into the petridish and make a flat surface wth a scalpel. Use dechlorinated or boiled and cooled water to adjust hardness of the potato medium.
Photos of each step with essential description, please @Navaneel
Also, i was wodnering about something. The most easily available pure cultures in the market which can be cultured comes from Yakult bottles. Since standardisation of the medium will require a stable replicating organism, and coconut water is still a natural uncontrolled process, we can use acified yakuly milk as a source of Lactobacillus casei Shirota strain.
Please restrict your description to what exactly, you had attempted the first time, with photos as evidence. @Navaneel
yes sir. I will send the photoes of the first time.
[11:44 AM, 9/2/2021] Arunan HBSCE: Let’s all understand through discussion.
Where exactly we need to improve. @Navaneel @Aditiya Joshi CUBE @Abhijeet Singh
[11:55 AM, 9/2/2021] Arunan HBSCE: With each photos please give essential description starting with objective in simple terms @Navaneel
Summary - SLBK A22
(subculture bottle of A line)

22 Days old Culture Bottle.

Date of Transfer Flies : 12/08/2021
Media Date : 11/08/2021
Transfer no of Flies : 1

Observation Based On Dates👇

12.08.2021 - Transferred 1 fly from A21 bottle to A22 bottle.

16.08.2021 - ~5 eggs, 0 larvae, 0 pupae, 1 fly

18.08.2021 - ~6 eggs, 0 larvae, 0 pupae, 1 fly

19.08.2021 - ~20 eggs, ~2 larvae, 0 pupae, 1 fly

21.08.2021 - ~20 eggs, ~3 larvae, 0 pupae, 1 fly

27.08.2021 - ~2 larvae, 2 pupae, 2 flies.

30.08.2021 - ~0 eggs, 1 larvae, 1 pupae, 1 fly, 1 dead fly.

01.09.2021 - ~1 fly, ~10 larvae (3mm) , ~8 pupae, 1 dead fly.
So what are your plans for these two cultures!?
Also are you able to see some microworms in these bottle culture? These are quite old cultures I think!
@Aswathy Suresh CUBE
What are we doing for saving A22!?
Do you think there is some problem with no.of flies in it!?
@Aswathy Suresh CUBE @Yash Sheregare CUBE @Man Masih Beck RANCHI CUBE
Let’s discuss on this!
What is your expectation in this bottle culture? @Aswathy Suresh CUBE And on what basis?

One Day After transferring microbes from Fermented Coconut water. Boiled Potato Slices replace to Nutrient Agar, at CUBE Sophia HomeLab, South Mumbai, July 21, 2021 From CUBE Archives mcarunan
Two Days After transferring microbes from Fermented Coconut water. Boiled Potato Slices replace to Nutrient Agar, at CUBE Sophia HomeLab, South Mumbai, July 22, 2021 From CUBE Archives mcarunan
Sir could you please say how you made the potatoes ?

Close up picture of colonies, two Days After transferring microbes from Fermented Coconut water. Boiled Potato Slices replace to Nutrient Agar, at CUBE Sophia HomeLab, South Mumbai, July 22, 2021 From CUBE Archives mcarunan
Control Three Days After Without transferring microbes from Fermented Coconut water. Boiled Potato Slices replace to Nutrient Agar, at CUBE Sophia HomeLab, South Mumbai, July 23, 2021 From CUBE Archives mcarunan

VIDEO SHARED BY ASWATHY AT 12.30PM
Yes @Abhijeet Singh observed some microworms are started to growing in SLBK E16 bottle (43 days old culture bottle) Video taken on 31.08.2021.
What is your comment on this picture? @Navaneel
Does this colony tell us anything based on colony characteristics and the source being fermented coconut water? @Navaneel Let’s look for some reference.
Let’s collaboratively move forward, by constructing knowledge, together.
Sir I was asking how you made the potatoes. Are they boiled, and how were the kept. Were the microbes from the broth which is growing there, or microbes fro air which later inoculated into the streaked coconut water
Are you finding the results interesting?

Or, does it look terrible?

Any interpretation from your side? @Navaneel
How about close up of other bottles, while searching for Nematode looking worms? @Aswathy Suresh CUBE
Why do you need so many steps will not boiling the potato be enough to kill all germs@@Navaneel
[1:36 PM, 9/2/2021] +91 28389: We will need to cool down the potatoes in air
[1:36 PM, 9/2/2021] +91 28389: If we don’t wash the potato properly, then the dirt will mix with the boiling water
[3:42 PM, 9/2/2021] Batul Pipewala Kolkata: @Navaneel you have a potential collaborator in Aashi who is in telegram CUBE grp she is a kalpana fellow interested in micro biology work.So please share your work in telegram Cube grp too
[3:46 PM, 9/2/2021] Batul Pipewala Kolkata: Join here Telegram: Join Group Chat
Also in short can you tell us what us the condition of cultures of @Yash Sheregare CUBE @Man Masih Beck RANCHI CUBE @Rechel Turkey Cube Ranchi Moina !?

Please post your homelab updates @Yash Sheregare CUBE @Man Masih Beck RANCHI CUBE!
Ok mam
Invitation to join today’s CUBE chatShaala,
Please add name of your PLACE/AFFILIATION along with the FULL NAME while joining, this would help us in making map of participants. Map will also be shared in all CUBE what’s app groups and on metastudio after the session

Here’s the direct link of the CUBE webinar: https://webinar.hbcse.tifr.res.in/b/kir-hye-cn4

Timing:5.30-9.30pm
Looking forward to your participation
Thanks.

Phyllanthus plants at Home Bhabha Center CUBE LAB are completely awake at 4.57pm today (2nd September 2021)
Accumulated water in an empty fallen coconut shell. Is this could be A possible breeding site of Aedes mosquitoes . Date of photo: 02nd September 2021. HBCSE CUBE LAB. @Abhijeet Singh @Gaurangi Dhuri @Saida Sayyed @Aswathy Suresh CUBE @Arunan HBSCE and others any comments. If this could be the possible breeding site then how do we prevent it?

Can we any sign of their End point of wakefulness and starting point of sleep Time: 5.20pm Date: 02nd September 2021. CUBE LAB HBCSE MANKHURD MUMBAI @Lakshmy PJ CUBE and othersYes definately this could be a possible breeding site for mosquitoes! If we see such accumulation of water we should remove the water and put the empty shell in dustbin!
Nice suggestion

Is there any way that we can find out which type mosquitoes can lay eggs here?
Yess … it can possibly be a aedes breeding site
BREAKING NEWS Revival of CUBE LAB HBCSE MANKHURD MUMBAI

. 5 traps were kept at different places of HBCSE like 1. Dustbin area near HBCSE canteen 2. Dustbin area in Garden 3. CUBE LAB 4. HBCSE hostel. Tomato was used as a bait and traps were kept at 4pm today in the evening. Date of photo: 02nd September 2021 CUBE LAB HBCSEHow do we find it out?
By checking whether the mosquito larvae are present there… bz I think it would be difficult to find eggs in the shell…
@Aswathy Suresh CUBE @Lakshmy PJ CUBE @Gaurangi Dhuri @Abhijeet Singh @Saida Sayyed and others kindly join the webinar
After a long time! All the best! Is it Home Bhabha Center or Homi Bhabha Center for Science Education?

Yes Indeed
It’s CUBE LAB HBCSE TIFR
CUBE Ranchi ReMans’ Home Lab
02-09-2021
Fruitfly Update

Immediate Objective: to start a single line fruitfly culture(RNSL-B) line at ReMan’s Lab on TRSV Media

Short Term Objective: -To identify the trapped fruitflies
-To find native Drosophila melanogaster species from Ranchi

Media preparation- 18-08-2021
Transferred date- 19-08-2021

OBSERVATION
after 14 days of transfer
•Larvae ~ 5
•Pupae ~ 93
•Fly ~ 30

Highlights
•flies first appeared on 28th Aug(9th day after transfer)
•most larvae have turned into pupae
•all flies seen have red eyes
@Man Masih Beck RANCHI CUBE please join in Chatshaala. Interesting discussion on Fruitflies is going on. Please join.

KiranKalakotiR · September 3, 2021, 8:24am

2nd September 2021, 9.30pm to 3rd September 2021, 9.30pm

The 2017 Nobel Prize in Physiology or Medicine
jointly to
Jeffrey C. Hall, Michael Rosbash and Michael W. Young
for their discoveries of molecular mechanisms controlling the circadian rhythm

VIDEOS SHARED BY ASWATHY AT 1.34AM
Fruitfly Update

23 Days old culture bottle have ~3 Larvae, 14 Pupae, 1 Fly.

SLBK A22
Transfer Date : 12.08.2021
Transfer Flies : 1 Fly

Objective : To Find Drosophila Melanogaster from Native area.

03/09/2021
(observation today)

No of Flies : 1
No of Larvae : 3
No of Pupae : ~14
No of Dead Flies : 1
Media Condition : Solidified

Update : 03/09/2021
Location : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
Fruitfly Update

23 Days old culture bottle have ~20 Larvae, ~80 Pupae, ~25 Flies.

SLBK B21
Transfer Date : 12.08.2021
Transfer Flies : 12
Gravid Flies : 6 ; Male Flies : 6

Objective : To Find Drosophila Melanogaster from Native area.

03/09/2021
(observation today)

No of Flies : ~25
No of Larvae : ~20
No of Pupae : ~80
No of Dead Flies : ~20
Media Condition : Solidified

Update : 03/09/2021
Location : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
Fruitfly Update

23 Days old culture bottle have ~20 Larvae, ~120 Pupae, ~65 Flies.

SLBK E17
Transfer Date : 12.08.2021
Transfer Flies : 14
Gravid Flies : 6 ; Male Flies : 8

Objective : To Find Drosophila Melanogaster from Native area.

03/09/2021
(observation today)

No of Flies : ~65
No of Larvae : ~20
No of Pupae : ~120
No of Dead Flies : ~30
Media Condition : Solidified

Update : 03/09/2021
Location : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
Microworms present in SLBK A21 culture bottle. Date of observation 03.09.2021 : 12:45am. Further plan is to transfer this microworms from this culture to separate culture media bottle in order to identify them.
How can we transfer microworm from the media bottle @Aswathy Suresh CUBE
In yesterday’s Chatshaala we discussed the role of genes of circadian rhythm and their function discovered by scientists for which 2017Nobel prize was won.
The gene called period or per gene is controlled by negative feedback meaning when per gene switches on and forms per protein it will itself switch off the gene and prevent more per protein from being formed.During the day time per is degraded by the sunlight and hence cannot switch off its production and will continue to be made.
@Aswathy Suresh CUBE @Abhijeet Singh @Lakshmy PJ CUBE @Saida Sayyed @KIRAN CUBE and others

Objective:- prediction and prevention of mosquito born diseases. Yesterday i.e. 2/09/21 I got 6 mosquitoes. None of them are Aedes. Noted the observation in log book. Here is the photo of mosquitoes. Location:- Ulwe, Navi-Mumbai. Cube locality lab Ulwe.
@Gaurangi Dhuri please post your updates on mosquito studies!
@Aswathy Suresh CUBE @Batul Pipewala Kolkata @Seema @Shraddha Sonavane CUBE @Hina Mudgal Kanpur CUBE please post your updates like how are you planning for starting mosquitoes project in collaboration with us! If you have started please post your updates too!

Let’s have the data from different part of the country!
Equinox is approaching!
We can use brush - wet paint brush will help for to transfer worms in a new media.
Please add your comments on the photo @Sunita Chahar CUBE Ma’am!
Did you got more mosquitoes with time!?
What the situation of mosquitoes in your locality!?
Iam planing to take this flies for further experiments.

In SLBK E16, E14 subculture bottle I found some microworms started to growing. But I couldn’t able find out in E15 bottle.

Yes, E16 - 44 days old culture. E15 - 60 days old culture. E14 - 93 days old culture. These are quite old cultures.
Which species is this? Any guess? At what time of the day did you click this pic? Can you give details plz…
In A22 bottle currently have only 1 fly, ~14 pupae , ~3 larvae. So I am waiting for to get more progeny’s from this bottle. After to make more subculture at same time for to save A line.
Can you think of any reason of not finding worms in E15 bottle…
Expectations

transferred one fly, if it is proper gravid fly expecting 50 eggs per day.
So, 23rd Day expecting ~500 eggs, ~100 Larvae, ~300 Pupae, ~70 Flies.
[11:24 AM, 9/3/2021] Abhijeet Singh: So washing with water is not enough?
Soap water, ethnol and all are necessary?
I think if we just wash the potatoes with enough water so that the dirt goes will be enough.
As far as the germs and microbes in that dirt is concerned, I think they will eventually die when you are boiling the potatoes?
What is your interpretation on this @Navaneel @Aditiya Joshi CUBE
Also what is your interpretation on ‘Homelabs’ @Navaneel ?
If I don’t have ethnol then I won’t be able to do it? Or anyone!?
[11:28 AM, 9/3/2021] Abhijeet Singh: I think we need go through some references for that.

Aedes mosquitoes lays eggs in clean water and I think in this would be clean enough for them to lay eggs. Not Sure!

National Vector Borne Disease Control Programme (NVBDCP), Telangana, Dr. S. Prabhavathi said that while Aedes Aegypti genus mosquitoes breed in clean water, Anopheles and Culex genus mosquitoes breed in dirty water. However, the official said, Anopheles Stephensi which carries malaria-causing parasite in urban localities, breeds in waters stored in containers

Because ma’am, this microworms are really small, I can only able to see when they curling in bottle side. Even if they are present in media,we can’t find out them.

E15 bottle is quite old culture and bottle wall is almost covered with empty pupal shells, dead flies etc . I checked them using proper light. But I couldn’t able to find out them in bottle wall area. May be they are present inside the media I guess.
How do we explain this?

So many pupae and no new flies? @Yash Sheregare CUBE @Aswathy Suresh CUBE @Lydia cUBe Drosphila @Saida Sayyed @KIRAN CUBE @Aastha Ahuja Cube
[11:29 AM, 9/3/2021] Dr. Sarita kumar Delhi CUBE: Yes…it’s a known fact…
[11:30 AM, 9/3/2021] Dr. Sarita kumar Delhi CUBE: E14 is older than E15…
@Navaneel please post your 2nd day observations with objective!
What did you expected and what did you get!?
Please Post your inference based on your expectations and observations in short with each setup!
Looking forward…!
Very interesting @Aswathy Suresh CUBE

When were you expecting fresh flies after observing so many pupae? @Aswathy Suresh CUBE
When did you start observing this bunch of pupae in SLBK A22? @Aswathy Suresh CUBE @Yash Sheregare CUBE
Are you happy? @Aswathy Suresh CUBE
Please describe, why.
Yes sir, I got one new progeny’s in between but That was died after some days .
Currently,~14 Pupae present inside culture bottle all are newly formed pupae. So I expecting next generation flies tomorrow & day after tomorrow.
Are you happy with this culture SLBK B and why?@Aswathy Suresh CUBE

What is your immediate plan of work? @Aswathy Suresh CUBE
Amazing capture! @Aswathy Suresh CUBE did you capture this through phone microscopy or normal phone camera adjustments!?
E15 - 60 days still have flies, Larvae, Pupae. E14 - 93 days old culture have no flies, no larvae, no pupae.

E14 bottle is much easy to check microworms because that bottle quite look empty.
Interesting! @Batul Pipewala Kolkata What exactly is negative feedback in gene expression and what is its implication?

Let’s explain this separately as a topic for all Cubists to grapple. @Batul Pipewala Kolkata

Another point is
surely, there must be some references you are banking on. Please post with an introduction how to read that reference article and which part is relevant in that reference article.@⁨Batu@Batul Pipewala Kolkata

Observed a Mimosa plant with beautiful flowers in my locality Mahad, Raigad; today morning at 7:30 a.m. Started the day with seeing its response to touch!

Expecting next generation flies on 04/09/2021 or 05/09/2021.
PREDICTION AND PREVENTION OF MOSQUITO BORNE DISEASES
A great idea to pursue in All India level and in Sri Lanka @Abhijeet Singh
What is your idea to rope in cubists from across the country before Autumnal Equinox, September 22,23
[11:48 AM, 9/3/2021] Arunan HBSCE: How do we improve the photo quality to make sure the features we are looking for is clear? @Abhijeet Singh @Gaurangi Dhuri @KIRAN CUBE @Raj Yashwantrao Patil CUBE @Ishita Assam @Aastha Ahuja Cube
[11:49 AM, 9/3/2021] Arunan HBSCE: What is special about Equinox? @Abhijeet Singh And what is it?
Recently, around 01.09.2021 observed ~8 pupae in A22 bottle.

Before that I got 2 pupae 24.08.2021 & 1 new fly on 27.08.2021.
Please post appropriate videos for all to review and not miss it.

We had earlier experience that SOMETHING WE MAY MISS, BUT OTHERS POINT THIS OUT TO US
@Aswathy Suresh CUBE
Yes, Now I don’t have much worry about A line culture. Because, at lest got some larvae & pupae inside A22 subculture bottle. So, I am expecting some new flies will emerges today or day after tomorrow as well.
[11:55 AM, 9/3/2021] Arunan HBSCE: When did you first notice the pupae so that you can expect when exactly, flies can emerge @Aswathy Suresh CUBE
[11:57 AM, 9/3/2021] Arunan HBSCE: Please post close up pictures of these precious SLBKA Pupae @Aswathy Suresh CUBE
Please describe how they look, according to you and when you expect them to give rise FRESH FLIES.
We must have some Expectation based on the day you started seeing LARVAE TURNING TO PUPAE😇 @Aswathy Suresh CUBE
Taken this video using normal phone camera, first focused area, then adjusted and zoomed at particular part & taken video.
So, basically you are not able to locate flies in E15, though it may have…right?
Actually ma’am , E15 culture still going culture… Mess up with lots of pupae (Hatched & Unhatched Pupae Shell) & dead flies. This case I couldn’t able to locate microworms inside culture.
[12:38 PM, 9/3/2021] Batul Pipewala Kolkata: Yes please post your observations on interesting work you are doing @Navaneel
[12:41 PM, 9/3/2021] Batul Pipewala Kolkata: Reference:https://www.nobelprize.org/prizes/medicine/2017/press-release/
@Abhijeet Singh can you take a close up photo of one mosquito each and place them to be able to see their back so markings on thorax can be seen

VIDEOS SHARED BY YASH AT 1.12PM
Updates on Fruit fly cultures
Location - Prabhadevi, Mumbai
Breaking News
SLPbA3
Highlight - On 7th day from transferring 3 fruit flies, there are no larvae seen today.
Alert - There are some black fungi like structures Growing in this media
I am suspecting that While transferring fruit flies from SLPbA2 culture, there could be possibility of transfer of unwanted fungi that was seen near the cotton plug

Media date* - 26/08/2021
Transfer date - 27/08/2021
No of flies transferred on 27/08/2021 - 3
Observation - No Larvae or pupae seen still on 7th day

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality
Immediate plan to save SLPbA3
This is what I think can be done

Transferring the 3 fruit flies to a new medium TRS-V media bottle - (I have additional one TRS-V media bottle that was made on 26/08/2021)
Observing if the fruit flies lay eggs in the new media as they haven’t yet in SLPbA3
culture
Any other suggestions from CUBists? @Aswathy Suresh CUBE @Man Masih Beck RANCHI CUBE @Isha Pawle @Abhijeet Singh @Arunan HBSCE sir @Sidhy Kerala Drosophila and others
Please post close up photos for others to check @Aswathy Suresh CUBE
Have we standardised the TRSV medium preparation?
Have you followed that? @Yash Sheregare CUBE In that case, how do we explain for the presence of fungi? @Yash Sheregare CUBE @Aswathy Suresh CUBE @Sidhy Kerala Drosophila @Aastha Ahuja Cube @Man Masih Beck RANCHI CUBE @Rechel Turkey Cube Ranchi Moina
Updates on Fruit fly cultures
Location - Prabhadevi, Mumbai
SLPbB3 - 7th day
Highlights - SLPbB3 shows 40 pupae and a ~ 20 3rd instar larvae on 7th day of transfer of fruit flies

Media date* - 26/08/2021
Transfer date - 27/08/2021
No of flies transferred on 27/08/2021 - 30

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality

3/09/2021
No of flies - 28
No of larvae - 20- 3rd instar larvae, - 10- 2mm larvae near media
No of pupae - 40
No of dead flies - 2
What is the summary of all cultures at CUBE Prabhadevi with BREAKING NEWS? @Yash Sheregare CUBE
Are you happy? @Yash Sheregare CUBE
We cannot understand what exactly is the sum of the whole story of Single Line Cultures at CUBE HomeLab Prabhadevi @Yash Sheregare CUBE

Surely, you can help by starting with Breaking News @Yash Sheregare CUBE
[1:37 PM, 9/3/2021] Yash Sheregare CUBE: Updates on Fruit fly cultures
Location - Prabhadevi, Mumbai
SLPbC3 - 7th day
Breaking News Highlights Only 5 pupae and 5- 3rd instar larvae seen on 7th day of sub culture on 27/08/2021

Media date - 26/08/2021
Transfer date - 27/08/2021
No of flies transferred on 27/08/2021 - 35

Short term Objective - 1) Culturing Single species Native fruit flies
2) Identify and culture Drosophila melanogaster species from my Locality

3/09/2021
No of flies - 33
No of larvae - 5 mm larvae
No of pupae* - 5
No of dead flies - 3

Expectations
31/08/2021 - Pupae and larvae - 10-20
*1/09/2021 - More pupae - 20+
3/09/2021 - More pupae - 30
4/09/2021 - Newly hatched fruit flies
[1:43 PM, 9/3/2021] Yash Sheregare CUBE: Yes I have followed the exact protocol followed by Other collaborators for making TRS-V Medium

The presence of Fungi can be explained as 1) in previous culture that is SLPbA2 ( - there was this black fungi seen in the culture and also near cotton plug
2) So while fruit flies were transferred, I am suspecting that the fungi was also transmitted along with fruit flies
3) Another Goof up I think is that the SLPbA2 (previous culture) had lose cotton plug so probably that can also be a reason for fungi like contaminants to enter inside the Media.
@Arunan HBSCE sir @Isha Pawle @Aswathy Suresh CUBE @Man Masih Beck RANCHI CUBE @Sidhy Kerala Drosophila
Does that mean that the antagonistic property of vinegar is for a period of time and not throughout the maintenance of an culture bottle?
Since it seems @Yash Sheregare CUBE suspects that fungi were stuck to cotton plug and may have developed later when anti fungal agents lost their effects.
@Aswathy Suresh CUBE @Yash Sheregare CUBE

KiranKalakotiR · September 6, 2021, 7:47am

3rd September 2021, 9.30pm to 4th September 2021, 9.30pm

Date:25/8/2021
Time:6:57pm
Place: Mampilly
Floral bud started developing+ 4th compound leaf arising same time.
Breaking news…pupae stop developing… trapped flies on 8.30pm on 29/07/2021… after that I seen some larveon 1/08/2021 it’s a open bait setup in which I used tomato and cucumber as a bait… after that I seen some big larve on 4/08/2021 it’s a housefly larve… in that setup I seen two types of larve some are 3 mm in size and some are 5 mm in size I seen some pupue le on 5/08/2021 some are 3 mm in size and some are mm in size after that I waiting for new flies some housefly emerge and one fruitfly emerged on 08/08/2021 but there are so many pupae you can seen here they stopped developing I couldn’t seen from 15 days when I seen today I found to that are not developing…

… and why? …
Location: wardha
Data collector: janhvi deshmukh
Time:2.31 pm
Janhvi deshmukh homelab bajaj collage of science Wardha
@Arunan HBSCE sir @Aswathy Suresh CUBE @KIRAN CUBE @Batul Pipewala Kolkata mam @Yash Sheregare CUBE and others
Some fruitfly pupae and some housefly pupae…@Arunan HBSCE sir @Yash Sheregare CUBE @Aswathy Suresh CUBE @Batul Pipewala Kolkata mam and others
Some dead housefly and some fruitfly pupae…@Arunan HBSCE sir @Yash Sheregare CUBE @Aswathy Suresh CUBE @KIRAN CUBE mam @Batul Pipewala Kolkata mamGreat @Lakshmy PJ CUBE
How many days does this plant take to develop first Floral bud
Long term objective -Floral dip method
Short term objective -

To observe life cycle
observing heteroblasty(To observe the transformation from simple to compound leaves.
3)Identifying cardamine plants

Date:27/8/2021
Time:1:54pm
Place: Mampilly
Flower of cardamine + 4th compound leaf.

Same point in which cardamine flower and compound leaf arising.
Date:28/8/2021
Time:6:30pm
Place: Mampilly
Fruit started developing
Date:29/8/2021
Time:6:12pm
Place: Mampilly
The fruit is developing + New floral buds developing
Date:31/8/2021
Time:6:02pm
Place: Mampilly
The fruit is developing.
Date:31/8/2021
Time:6:02pm
Place: Mampilly
Colour change noted on leaves.
Date:1/8/2021
Time:6:28pm
Place: Mampilly
The fruits is developing.Colour change noted on leaves.
Date:1/8/2021
Time:6:28pm
Place: Mampilly
The fruits is developing.Colour change noted on leaves.
Date:3/8/2021
Time:3:06 pm
Place: Mampilly
3 fruit on one single plant. The fruit development in a dichotomous manner.
Cardamine plants in coconut shell.Date:3/8/2021
Time:3:06 pm
Place: Mampilly
Date:3/8/2021
Time:3:15 pm
Place: Mampilly
Flower bud and 4th compound leaf developing simultaneously.
27days later on germination.@KIRAN CUBE The seeds germinated on 30/7/2021.On 25/8/2021 first floral bud.
In how many Cardamine plants you have observed the floral bud after 27 days of seed germination?
[3:40 PM, 9/3/2021] Aswathy Suresh CUBE: Pupae first note in A22 bottle on 27.08.2021. and after 01.09.2021 observed ~8 Pupae.
[4:34 PM, 9/3/2021] Aswathy Suresh CUBE: What about other culture bottles @Yash Sheregare CUBE?
What is your further plan @Man Masih Beck RANCHI CUBE?
How many days old bait is this @Janhvi Deshmukh ? Did you tried with new bait, in order to trap fruit flies?
One month @Aswathy Suresh CUBE

VIDEOS SHARED BY VISHNUPRIYA AT 5.31PM
Update on ant model system .

Long term Objective: how does ants communicate?

Started ant model system on April 21st 2021 by feeding ants with honey and Later on
On 22nd April created ant formicarium for closer observation of ants .

But unfortunately due to health and other issues I couldn’t continue with it . And yesterday on 2nd of September I saw some ants under wet wooden logs . And they were orange yellow ish in colour .

Placed a jar filled with soil and sugar on top and today on 3rd September 2021 they
Completely covered the whole jar .

How do we identify what type of ant it is ? @ Seethalakshmi

Date : 3rd September 2021
Time : 5.29 pm
Location: alapad thrissur kerala
Collected by: vishnupriya cj cube sn nattika
[5:31 PM, 9/3/2021] Vishnupriya CUBE: Update on ant model system .

Long term Objective: how does ants communicate?

Started ant model system on April 21st 2021 by feeding ants with honey and Later on
On 22nd April created ant formicarium for closer observation of ants .

But unfortunately due to health and other issues I couldn’t continue with it . And yesterday on 2nd of September I saw some ants under wet wooden logs . And they were orange yellow ish in colour .

Placed a jar filled with soil and sugar on top and today on 3rd September 2021 they
Completely covered the whole jar .

How do we identify what type of ant it is ? @917994274817

Date : 3rd September 2021
Time : 5.29 pm
Location: alapad thrissur kerala
Collected by: vishnupriya cj cube sn nattika
[5:40 PM, 9/3/2021] Vishnupriya CUBE: Update on butterfly model system.

Till now ( 3rd September 2021) observed 26 species of butterflies in my locality.

Out of this list , life cycle of 7 butterflies were studied.
•common crow
• common mime
• peacock royal
•common mormon
• common emigrant
• commander
• pysche butterfly

Small term objective : study life cycle of butterflies
And butterfly diversity

Long term Objective:
What exactly is mimicry? study more about butterflies that show mimicry .

Date : 3rd September 2021
Time : 5.43 pm
Location: alapad thrissur kerala
Collected by : vishnupriya cj cube sn nattika
Update on butterfly model system

Keerthana balakrishnan of SN college nattika currently pursuing her degree in malayalam language was so much interested to know more about butterflies and started to observe a lot more than before .

And its so good to see people from other fields showing interest in these things .
Today her 1st pupa hatched and its a papilio polymnestor ( blue mormon) . Video created by keerthana balakrishnan
Date : 3rd September 2021
Time : 5.50 pm
Collected by: keerthana balakrishnan sn college nattika

Single line culture bottles of fruit flies. TRSV medium prepared on 15/08/2021. Transferred gravid fly on 16/08/2021.
There is fungal growth only in SLTT - J bottle. Lost that culture. Fungal growth was observed from 14 th day. In the same bottle , the number of pupae and new generation flies emerged is less. Only five new generation flies formed. One fly got stuck in the medium on the same day it emerged. Other two young flies and mother fly died on 16 th day (There had fungal growth in the medium). Two flies were remaining on 02/09/2021.Not gravid flies. Before transferring them to medium bottle , I tried to transfer them to an empty bottle to remove(complete removal not possible) the fungal particles stick to their body. Because , the flies were walking through the fungi. While transferring , one fly escaped. Remaining one was a small fly. I think it was male. Even though the new medium was ready , I didn’t transfer the fly into the medium. That fly also died. So, lost the SLTT-J culture.

03/09/2021
Thanniam, Thrissur, Kerala
Sidhy.P.P , CUBE S N College, Nattika.

TRSV medium prepared on 02/09/2021 to subculture SLTT-H , SLTT-I and SLTT-J. But there are no J flies to transfer. Lost that culture.
03/09/2021
Location: Thanniam , Thrissur , Kerala.
Sidhy.P.P , CUBE S N College, Nattika.

I found something interesting in my courtyard. Today on 3rd of September 2021.

That place is completely filled with weeds ( green colour) maybe a weed called Japanese stiltgrass.( not sure. If wrong pls do correct me)
But I came to notice one among them was different. It had a white and green colour combination on its leaf and was so beautiful and unique from others .

Why did that particular one have a colour variation . It has got 3 branches and all the 3 coming from the root are having green and white colour combination

Why there’s a change on its colour ? .

Time: 7.25pm
Date : 3rd September 2021
Location: alapad thrissur kerala
Collected by: vishnupriya cj cube sn nattikaI found something interesting in my courtyard. Today on 3rd of September 2021.

That place is completely filled with weeds ( green colour) maybe a weed called Japanese stiltgrass.( not sure. If wrong pls do correct me)
But I came to notice one among them was different. It had a white and green colour combination on its leaf and was so beautiful and unique from others .

Why did that particular one have a colour variation . It has got 3 branches and all the 3 coming from the root are having green and white colour combination

Why there’s a change on its colour ? .

Time: 7.26pm
Date : 3rd September 2021
Location: alapad thrissur kerala
Collected by: vishnupriya cj cube sn nattika
It looks like the green colour chlorophyll is expressed in some parts and not expressed in other parts giving it a pattern of green and white.

KiranKalakotiR · September 6, 2021, 7:47am

4th September 2021, 9.30pm to 5th September 2021, 9.30pm

Close up pictures of A22 subculture bottle. ~10 more pupae + ~2 larvae observed today. Expecting new flies will emerg today/tomorrow. Picture taken 03/09/2021 : 11:09pm.

VIDEOS SHARED BY ASWATHY AT 11.40PM TO 1.44AM
[11:40 PM, 9/3/2021] Aswathy Suresh CUBE: Closeup video of E15 subculture bottle @Dr. Sarita kumar Delhi CUBE ma’am @Arunan HBSCE sir… We can able to see lot of dead flies body’s accumulated in bottle wall side and pupae shell also can able to see inside the culture bottle. Can’t able to see any microworms in bottle wall area.
[11:52 PM, 9/3/2021] Aswathy Suresh CUBE: Close up pictures of A22 subculture bottle. ~10 more pupae + ~2 larvae observed today. Expecting new flies will emerg today/tomorrow. Picture taken 03/09/2021 : 11:09pm.
Fruitfly Update

24 Days old culture bottle have ~2 Larvae, ~10 Pupae, ~4 Newly Emerged Flies .

SLBK A22
Transfer Date : 12.08.2021
Transfer Flies : 1

Objective : To Find Drosophila Melanogaster from Native area.

04/09/2021
(observation today)

No of Flies : 4 Flies (Including mother fly)
No of Larvae : 2
No of Pupae : 10
No of Dead Flies : 1
Media Condition : Solidified media

Update : 04/09/2021
Location : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
Fruitfly Update

24 Days old culture bottle have ~10 Larvae, ~90 Pupae, ~27 Flies .

SLBK B21
Transfer Date : 12.08.2021
Transfer Flies : 12
Gravid flies : 6 ; Female flies : 6

Objective : To Find Drosophila Melanogaster from Native area.

04/09/2021
(observation today)

No of Flies : ~27
No of Larvae : ~10 (3mm)
No of Pupae : ~90
No of Dead Flies : 30
Media Condition : Solidified media.

Update : 04/09/2021
Location : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
Fruitfly Update

24 Days old culture bottle have ~15 Larvae, ~125 Pupae, ~55 Flies .

SLBK E17
Transfer Date : 12.08.2021
Transfer Flies : 14
Gravid flies : 6 ; Female flies : 8

Objective : To Find Drosophila Melanogaster from Native area.

04/09/2021
(observation today)

No of Flies : ~55
No of Larvae : ~15
No of Pupae : ~125
No of Dead Flies : ~40
Media Condition : Solidified media.

Update : 04/09/2021
Location : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
SLBK E15 - 62 Days old culture bottle (Transfer date of bottle 02.07.2021) still have ~10 flies, ~10 Unhatched pupae, ~2 Larvae .Video Date : 04/09/2021 ; 12:15am. Home Lab : Bottukadavu - Thrissur Kerala.
So actually, Aedes Mosquitoes have White stripes on their legs so we can differentiate between non-aedes and aedes.

What we do is:
After we catch the mosquitoes we keep them on a black background. If the Aedes mosquito is there then in the black background the white stripes are clearly visible.

That’s how we identify Aedes.
Ohh thanks for the information! I will also try to identify Aedes mosquitoes nearby.
There are two types of Aedes …Aedes aegypti and Aedes albopictus so it’s look different from each other @Abhijeet Singh
What is the difference?
I don’t know that’s why I am asking @Manadi Cube
[1:53 PM, 9/4/2021] Arunan HBSCE: Wah, wah! @Manadi Cube
[1:54 PM, 9/4/2021] Arunan HBSCE: Great @Abhijeet Singh Any reference? @Abhijeet Singh @Manadi Cube @Gaurangi Dhuri
[1:55 PM, 9/4/2021] Abhijeet Singh: Actually we have gone through the Aedes species. We just see the stripes if present.
[1:56 PM, 9/4/2021] Abhijeet Singh: We have to check more on that
Firstly, how do we collect mosquitoes from our surroundings?
Can we also look around for Mosquito breeding sites? @Manadi Cube
Because Aedes aegypti and Aedes albopictus they both have white stripes …@Abhijeet Singh
Why not look for references and post the references here? @Janhvi Deshmukh @Abhijeet Singh @Manadi Cube @Hina Mudgal Kanpur CUBE and others
Yes sir @Arunan HBSCE sir

The circled once are Aedes. On a black background the stripes are visible. This pic is from 2017 National meet Presentation.
So another are anopheles? @Abhijeet Singh
[2:01 PM, 9/4/2021] Abhijeet Singh: Not sure😅
[2:02 PM, 9/4/2021] Abhijeet Singh: I posted the pic as a reference to how we can differentiate Aedes and Non-Aedes.
Online Healthcare & Medical Courses - FutureLearn difference between Aedes aegypti and Aedes albopictus but the look so similar

Ok @Abhijeet Singh
[3:17 PM, 9/4/2021] Manadi Cube: According to my knowledge, mosquitoes breed in stagnant water. So we must look for mosquitoes in stagnant water.
[3:18 PM, 9/4/2021] Manadi Cube: Around stagnant water*
Legs as well as abdomen
@Janhvi Deshmukh …See this picture. One is of Aedes albopictus nd one is of Aedes aegypti. Observe the difference and try to find out, which one is which…
Which one is Aedes aegypti mam @Dr. Sarita kumar Delhi CUBE mam ?
[3:49 PM, 9/4/2021] Dr. Sarita kumar Delhi CUBE: Left one…look for references now.
[3:51 PM, 9/4/2021] Dr. Sarita kumar Delhi CUBE: https://www.oecd-ilibrary.org/sites/9789264302235-5-en/index.html?itemId=/content/component/9789264302235-5-en

Read through👆
Also, how do you look for Aedes mosquito without being bitten by it? @Abhijeet Singh

[4:48 PM, 9/4/2021] Sunita Chahar CUBE: Phyllanthus grown by seeds
[4:49 PM, 9/4/2021] Sunita Chahar CUBE: 4 to 5 leaves in 14 days
[4:50 PM, 9/4/2021] Sunita Chahar CUBE: After germination
[4:50 PM, 9/4/2021] Sunita Chahar CUBE: Seeds took almost 8 to 10 days to germinate
The one fly that I transferred on 12.08.2021 from SLBK A21 to A22 bottle, that time not much sure it’s gravid or not.

6 days later, after transferred that one fly (18.08.2021) - Eggs were started present inside te culture bottle.

(7 days after) Next day 19.08.2021 - Observed 2 (3rd instar) larvae inside bottle wall. Transferred fly still alive.

15 days after (27.08.2021) - there is 2 flies (including transferred fly).

21 days later (01.09.2021) observed ~8 Pupae ~3 larvae 1 alive fly. & 1 dead fly.

(This data based on observation)

Goof ups : observation in between missed (e.g : first fly emerged date, first pupae, first 1st instar larvae etc).[5:04 PM, 9/4/2021] Aswathy Suresh CUBE: I think yes, because that transferred one fly also laying Eggs (~20) and give some Larvae (~3) , Pupae (~2) inside culture. But the issue is that it’s taken more days to hatch to from larvae then larvae to pupae…
[5:37 PM, 9/4/2021] Aswathy Suresh CUBE: Yes sir, around these days I am very happy about SLBK B culture because currently its have good number of Larvae (~20), Pupae (~80), Adult flies (~20).
"Genetics of Aquatic Organisms2021, Vol 5, Num, 2 (Pages: 77-85) Abstract Full Text:PDF Recommended articles Mail to Author How to Cite
A Cost-effective DNA Isolation Strategy from Crustaceans Enables the First Molecular Phylogenetic Identification of Moina macrocopa from India
Shital Bhanushali 1-2 ,Kritika Katti 2 ,Jyoti Ramchandani 2 ,Subhojit Sen 1
1 Mumbai University, School of Biological Sciences, UM-DAE Centre for Excellence in Basic Sciences, Kalina Campus, Santacruz (E), Mumbai 400098
2 University of Mumbai, Department of Life Sciences, Vidyanagari, Santacruz (E), Mumbai 400098
DOI : 10.4194/2459-1831-v5_2_04
Viewed : 417 - Downloaded : 154
We report the first barcode identification of water flea species Moina macrocopa (strain JSK1) from th…
NATIONAL CUBE MEET-2021
(October 1st-3rd 2021)

Hello everyone,

We’re so excited to share the news that we’re organizing NATIONAL CUBE MEET -2021 and this time the month of the National meet is also coinciding with the announcement of Nobel Prize 2021.

The event will be of three days from October 1st -3rd 2021 in the evening from 5.30pm to 9.30pm and the program will also be broadcast live on YouTube

Save the dates
1st Oct 2021-Friday
2nd Oct 2021-Saturday
3rd Oct2021- Sunday
Timing: 5.30pm to 9.30pm

EVENT DETAILS WILL BE SHARED SOON

Close -Up view of culture ant. Iam observed 3 hairs in the head part. And its legs and antenna is long. This is also an identical feature. Body colour is brownish black. Some times it shows blue in colour.
Location: pazhuvil
Date: 1/9/21
Time:3:29 pm
Data collector: Seethalakshmi

KiranKalakotiR · September 6, 2021, 8:32am

5th September 2021, 9.30pm to 6th September 2021, 9.30pm

It’s a white moth? Looks beautiful … Data collector: janhvi deshmukh … time:10.05 pm. Date :04/09/2021

@Aswathy Suresh CUBE in the culture bottle SLBK A22 there are new progenies including ~ 2 larvae , ~10 pupae , ~4 new flies. So, are they progenies of the that mother fly which was transferred to make single line?
Actually @Rechel Turkey Cube Ranchi Moina,
SLBK A21 subculture bottle I transferred ~5 flies from A20 bottle after few days later only one fly were left in SLBK A21 subculture bottle, other flies were died and didn’t get any next generation progeny’s from that bottle.That one fly I transferred in to A22 bottle (current subculture bottle).
Rechel’s CUBE Homelab morabadi Ranchi

Date: 04/09/2021

Fruit fly report

Currently having 3 native fruit fly culture bottles : RF-IIb , RF-IIC, RF-IIC’.

RF-IIb :transfer date(TD) 19/06/2021
Media Date (MD) 06/06/2021 ~ 30 flies present. Pupae and larvae are present.

RF-IIC and RF-IIC’ :
TD and MD unknown
( Goofup : didn’t mentioned the date while transferring. But it was transferred in mid-july)

~ 50 fruit flies are present in both ( RF-IIC and RF-IIC’) culture bottles. Pupae and larvae are also present.

TOMORROW’S PLAN

To make TRSV medium culture bottles to make single line
(for Reman’s lab as well)

7 Bottles were washed on 16/08/2021.
Requirements for TRSV medium.
1 Tomato : medium sized
Rava : 2 table spoon (~30g)
Sugar : 1 table spoon (~15g)
Vinegar: 1 table spoon ( ~15ml)
Water : ~ 150ml

Collaborators: @Aswathy Suresh CUBE @Man Masih Beck RANCHI CUBE @Yash Sheregare CUBE@Sidhy Kerala Drosophila and others .

VIDEOS SHARED BY RECHEL AT 12.30AM
[1:19 AM, 9/5/2021] Aswathy Suresh CUBE: Immediate plan is to make new TRSV media and transfer these flies from this 25 days culture bottle to new fresh media bottle in order to maintain this cultures.
[1:25 AM, 9/5/2021] Aswathy Suresh CUBE: Yes sir, I am waiting for to get more number flies from A22, B21 bottles. After getting enough number then Iam planing to transfer these flies in to new media bottle.
[1:30 AM, 9/5/2021] Aswathy Suresh CUBE: On 30.08.2021 dead fly is quite small, looking their abdomen it seemed Male fly.
[1:38 AM, 9/5/2021] Aswathy Suresh CUBE: Different stage larvae that I observed on 01.09.2021. Larvae started appear on bottle then only I started to noticed them…! Some earlier pupation date of formation also I missed out.

Yes, it is a kind of goof up that didn’t noted larvae earlier inside the culture. Not taken continues date data.
[1:45 AM, 9/5/2021] Aswathy Suresh CUBE: I think these all progeny’s came from this one fly that I transferred on 12.08.2021. (Even it may not be fully gravid fly).
Because I observed some eggs in bottle wall after transferred this fly. but it taken more time to get hatch to form larvae. Then larvae to pupae I guess.

VIDEO SHARED BY ARUNIMA AT 2.22PM
Date:5/09/21. Cardamine plant growing in open soil.a close relative of Arabidopsis thaliana.Belongs to the family Brassicaceae (mustard family)

New Phyllanthus experiment to study
1.Phenology pattern of plant
2.sleep wake cycle
3.studying the Effect of light on opening and closing of leaves once again

[5:46 PM, 9/5/2021] Sunita Chahar CUBE: Researchers recycle shrimp waste into supplements
[5:47 PM, 9/5/2021] Sunita Chahar CUBE: Can we recycle prawns without chemical treatments

I put the seeds on the soil, on August 16. and they are started germinating on 22 agu .and on 23 there are 3 leaves and on 26th Aug there are 4 leaves.
Which seeds did you sow?
Phyllanthus maam.

There are very few leaves in this plant and they started fruiting.
At how many leaved stage, the plant flowered
As an aside: https://kids.frontiersin.org/
Maam 16,17 leaves are there

CUBE Ranchi ReMans’ Home Lab
05-09-2021
Fruitfly Update

Alert Alert Alert
Immediate Objective: to save the RNSL-B culture by transferring the flies into new TRSV Media bottle

New Media preparation- 05-09-2021, 6 am

Planned Day to transfer flies
06-09-2021; 6 am

OBSERVATION
-On 4th September morning, it was observed that the TRSV Media was turning liquid and thus many flies were dying.
-currently around 8 flies are seen but none seems to have a bulging body.

Highlights
•flies first appeared on 28th Aug(9th day after transfer)
•all flies seen have red eyes
•4th Sep- media turns liquid
•5th Sep- new media bottles prepared
@Rechel Turkey Cube Ranchi Moina @Aswathy Suresh CUBE @Yash Sheregare CUBEand others…
Good to see you here! @Sohail Keegan Pinto
[9:34 PM, 9/5/2021] Arunan HBSCE: What is your objective? @Arunima SN Nattika Cardamine
[9:38 PM, 9/5/2021] Arunan HBSCE: But fresh larvae and pupae came only after the second fly emerged. Is it not? Can you please tell us why it took so long for the larvae and pupae to emerge, if the mother was the original fly you transferred on 12 August? @Aswathy Suresh CUBE

We have been doing *MOSQUITO breeding sites survey.
*Sample collection by killing MOSQUITO after claptrap.
*Students have set MOSQUITO ovitraps, to identify the types of MOSQUITO breeding in the locality.
@ hina cube kanpur.I thinking that , the one fly that I transferred in to media bottle was not properly gravid at that time. But the fly were laying eggs not fertilised. After few days later observed 2 larvae and 1 pupae. And one new fly observed around 30th August , but I couldn’t found it is male or female. Because the fly were died very fast.

I am suspecting that dead newly fly maybe male fly. Because that time I observed only transferred fly & newly emerged fly There could be possible that these flies mated that’s why get more larvae & pupae inside the culture currently I guess.

VIDEOS SHARED BY ASWATHY AT 1.26AM
Fruitfly updates

25th Day : ~18 Flies, ~10 Larvae, ~90 Pupae.

SLBK B21

Transfer Flies Date : 12.08.2021
Transfer Flies No : 12

06/09/2021
Today observation

No of Flies : ~18
No of Larvae : ~10
No of Pupae : ~90
No of Dead Flies : ~30

Further plan
Make new media and transfer flies from current bottle to new bottle.

Update : 06/09/2021
Home Lab : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.
Fruitfly updates

25th Day : ~35 Flies, ~20 Larvae, ~120 Pupae.

SLBK E17

Transfer Fly Date : 12.08.2021
Transfer Fly No : 14 Flies
Gravid Flies : 6 ; Male Flies : 8

06/09/2021
Today observation

No of Flies : ~35
No of Larvae : ~20
No of Pupae : ~120
No of Dead Flies : ~40

Further plan
Make new TRSV media , transfer flies from this bottle to new media bottle in order to maintain E culture.

Update : 06/09/2021
Home Lab : Bottukadavu - Thrissur Kerala.
Update by : Aswathy Suresh CUBE SN Nattika.

Pan-pan, Pan-pan, Pan-pan
TRSV Media for Fruitflies, failed at ReMan’s Lab

Fruitflies Update
ReMan’s Lab Ranchi
06-09-2021

Due to liquification of TRSV media since 4th of September, emergency transfer of flies had done into new culture bottle RNSL-B2

Media preparation date- 05-09-2021
Transfer date- 06-09-2021
No. of fruitflies transferred- 6
Fruitflies with bulgy and pointed abdomen- 2

@Aswathy Suresh CUBE @Yash Sheregare CUBE @Rechel Turkey Cube Ranchi Moina and others.

Fruitflies on Corn! Date:- 6th Sept 2021. Time:- 10.36AM. Cube Locality Lab Ulwe, Navi Mumbai. Data collector:- Abhijeet Singh.
Caught 11 mosquitoes yesterday night as I was awake till 3am … Date- 5sept Time - 11pm-3am… No of mosquitoes- 11… No.of aedes- 0 Data collecter- Gaurangi Dhuri … Location - Tilak nagar, Mumbai
@Gaurangi Dhuri please can we get a clear close up of the mosquito showing its body features in more detail ,one mosquito at a time
Yess… I shall click one of the mosquito’s close up pic
Hello everyone

The broad guidelines of the presentations are out now. The time duration of presentation is 20min and there will be a discussion round after the presentation which is of 15 min for each presentation.

Take us to your home lab in this CUBE NATIONAL MEET-2021, hope to see everyone to start preparing for the presentation.

We can also meet in CUBE chatShaala (https://webinar.hbcse.tifr.res.in/b/kir-hye-cn4) to create connection in model systems.

Be ready to rock.

For more information kindly reply to this message or you can also message us personaly.

Thanks and All the very best👍

KiranKalakotiR · September 8, 2021, 8:41am

6th September 2021, 9.30pm to 7th September 2021, 9.30pm

Mango tree : 1
𝗦𝘁𝗮𝗿𝘁𝘀 𝘁𝗼 𝗳𝗹𝗼𝘄𝗲𝗿𝗶𝗻𝗴
Flowering is observed in many branches of the mango tree.
Date: 6/9/2021.
Time: 12:23pm.
Temperature : 29°C/24°C
Location : Pazhuvil ,Thrissur,Kerala.
Latitude : 10.419129.
Longitude : 76.1522749.
Data collector: Seethalakshmi
1:partying_face: Mangoes started flowering!

Mango flowers in full bloom Location :Tenkasi Tamilnadu Date 6.9.2021 Data Collector :Binumol & Shrudhiga

Date:6/9/21.Time 4 pm.objective: To identify the cardamine species. Cardamine with developing seed pods observed.CUBE home lab Kozhikode Kerala

Snail passing by road of HBCSE campus. Location: CUBE LAB Mumbai, Date of photo: 05/09/21

Snail at 4th Floor residence, Bhulabhai Desai Road, Mumbai 6 September 2021 Photo: mcarunan
Giant African snail Achatina fullica Cube Ghatkopar

Colour: cream
Location :Pazhuvil
Date: 30/5/21
Time: 1:30 pm
Size:~1cm
Data collector :Seethalakshm
Snail, at 4th Floor residence, Bhulabhai Desai Road, Mumbai 6 September 2021 Photo: mcarunan Looks surely different from the Giant African Snail in looks and size. Looking for comments.

VIDEO SHARED BY JANHVI AT 7.51PM

How is this different from the Giant African Snail? Snail at 4th Floor residence, Bhulabhai Desai Road, Mumbai 6 September 2021 Photo: mcarunan
iRAM:

Today I have trapped 23 earthworm and in one cup culture I have put 2 earthworm I have found then under the flower pots
This is worms
Yes
How many worms you have @~Iram
I have 23 earthworm
Iram, wonderful please join the CUBE Chatshaala by clicking http://hbcse.tifr.res.in/b/kir-hye-cn4
Hello @KIRAN CUBE can you please share today’s snapshot of snail whorls?
First of all, this snail doesn’t have a conical shell like Giant african snail.
And it has broad yellow stripes on the shell where as Giant african snail majorly has brown color and light yellow stripes
Now that’s new !! Cornflies??? Haha

KiranKalakotiR · September 8, 2021, 8:42am

7th September 2021, 9.30pm to 8th September 2021, 9.30pm

Rechel’s CUBE Homelab morabadi Ranchi

Fruit fly report

Made 3 TRSV medium culture bottles on 05/09/2021

Ingredients:

One Tomato ( medium sized)
Rava : ~ 30 g (2 table spoon)
Sugar: ~ 15g (1 table spoon)
Vinegar: ~5ml
Water : ~ 150 to 200 ml

Quantities were measured with a medicine cap.

After one day (on 06/09/2021) condition of media is good. There is no fungal growth.

Further plan : to transfer gravid fruit fly to make single line cultures.

@Man Masih Beck RANCHI CUBE @Aswathy Suresh CUBE @Yash Sheregare CUBE @Sidhy Kerala Drosophila and others
Yes!
So actually it’s just that when mosquito comes to bite we just catch that one. If we go to a place where more mosquito will be there in order to catch then possibly we can get bitten by mosquito.

So as soon as the mosquito comes to you we kill it and collect it. After that we identify.
Amazing!
[7:57 AM, 9/7/2021] Abhijeet Singh: The shape of shell.
In Giant African it’s somewhat conical. @Arunan HBSCE @Manasi CUBE
[7:58 AM, 9/7/2021] Abhijeet Singh: Yes exactly @Manasi CUBE
[8:22 AM, 9/7/2021] Arunan HBSCE: “After few days observed 2 larvae and 1 pupa”. Don’t you think this is an amazing Goof-up to be celebrated? @Aswathy Suresh CUBE
That even when you were keen to save the culture of SLBKA flies, you were not paying adequate attention to this ‘one fly bottle’ and there is no reporting with dates.
Why not celebrate this? @Aswathy Suresh CUBE Let’s write clearly *what made you not posting about this bottle culture which was looked forward to, by many collaborators to engage them? @Aswathy Suresh CUBE
Let’s celebrate.
[8:26 AM, 9/7/2021] Arunan HBSCE: How many days after seeing eggs in this bottle did you see 2 larvae and 1 pupa? @Aswathy Suresh CUBE
Do you have photos of these various stages? If so, please resend it to rebuilding the story.
It will help enormously.
If not, let’s celebrate it as Goof-ups. @Aswathy Suresh CUBE
Ohh using yourself as the bait! Haha:grin:
[10:02 AM, 9/7/2021] Aditiya Joshi CUBE: Needs to observe more number of trees in this and also different areas to confirm if this is onset of flowering season.
[10:02 AM, 9/7/2021] Aditiya Joshi CUBE: Whats the condition of Alstonia in various parts of India…
@Arunan HBSCE
@Aswathy Suresh CUBE @Batul Pipewala Kolkata @Lakshmy PJ CUBE @Seethalakshmy CUBE @Drishtant Cube Moina @P.chitralekha @Sunita Chahar CUBE and all others …

Alstonia or the devil tree… Flowering seems to be started on this tree… Rambaug, Kalyan west. 7/9/2021.

Another devil’s tree shown flowering buds… Rambaug, Kalyan west. 7/9/2021.
Devil’s tree I saw blooming in Kolkata on 5/9/21 will try and get pics@@Aditiya Joshi CUBE
Are you sure, you are able to save the SLBK A flies from a single fly, transferred on 12.08.2021?

In that case, why not announce as BREAKING NEWS for all to take stock of? @Aswathy Suresh CUBE
Why are you waiting for transferring into fresh culture bottles? @Aswathy Suresh CUBE
Please give a clear plan, to coordinate with all Fruitfly collaborators. @Aswathy Suresh CUBE
Any idea what is happening to cultures of @Sidhy Kerala Drosophila.
Please coordinate with @Sidhy Kerala Drosophila , @Yash Sheregare CUBE and the Ranchi and other groups too to organize for the CUBE NATIONAL MEET.
May be lighting from above would give clarity in pictures.
Can we take photos of the same sample in day light? @Gaurangi Dhuri
Why is it called devil’s tree?
Sorry I have never heard about it?
Can you please provide the common name?
And what is the tree used for?
Mango trees started flowering What percentage of Mango trees? @Seethalakshmy CUBE @Aswathy Suresh CUBE

This is marvelous! Even before Autumnal Equinox, lower Latitudes, like Thrissur10.4°N shows Mango trees flowering. @Seethalakshmy CUBE
What percentage of trees? What is the size of your sample, at Tenkasi, surveyed?

@Dr. Binumol SN Nattika @Shrudhiga
Or is it an odd tree flowering?

What is the Latitude& Longitude? @Dr. Binumol SN Nattika @Shrudhiga
Do you expect the Mango trees in lower Latitude than Thenkasi to flower too? @Dr. Binumol SN Nattika @Shrudhiga @Seethalakshmy CUBE Lets start organizing a systematic study with CUBE Nattika Fresher’s and others from Kerala and Tamil Nadu for presentation at CUBE National Meet, with data from northern Latitudes like Goa, Mumbai, Wardha, Kolkata and rest of North as Control. @Dr. Binumol SN Nattika @Shrudhiga @Seethalakshmy CUBE @Sucheta Naik Cube Goa @Batul Pipewala Kolkata @Aastha Ahuja Cube and others
What is your method to identify the Cardamine species.
Let’s design and involve all who have Cardamine HomeLab @Arunima SN Nattika Cardamine @Shraddha Sonavane CUBE @Lakshmy PJ CUBE and others
No mask? @KIRAN CUBE
Please give more details @KIRAN CUBE @Anjani Kumar Kashyap CUBE @Manasi CUBE and others
Please describe @Anjani Kumar Kashyap CUBE @Manasi CUBE

What are s the objective? @Anjani Kumar Kashyap CUBE @Manasi CUBE @Saida Sayyed
Wonderful @Seethalakshmy CUBE Please post a description.

Please post better photos with a ruler as reference for size @Seethalakshmy CUBE
[10:54 AM, 9/7/2021] Arunan HBSCE: Close up with description @Janhvi Deshmukh and a ruler or some reference for size measurement @Janhvi Deshmukh
[10:55 AM, 9/7/2021] Arunan HBSCE: Great! @~Iram
Please tell me s what do you expect in these cups?
[10:57 AM, 9/7/2021] Arunan HBSCE: Do we classify snails based on conical shape of shell vs other shapes for identification? @Manasi CUBE
[10:58 AM, 9/7/2021] Arunan HBSCE: Great to see Nishani from SriLanka, yesterday. @Aswathy Suresh CUBE
[11:00 AM, 9/7/2021] Arunan HBSCE: Great! @Rechel Turkey Cube Ranchi Moina
Please coordinate with all HomeLabs and please rope in fresh cubists, including from CUBE MHSS Goa
[11:04 AM, 9/7/2021] Arunan HBSCE: @Anuhya Mucheli:wink: Please give a summary of your work and of other cubists of Nellore, so far.
Let’s develop it to present at the CUBE National Meet @Tejasree @Ishita Assam and others
What percentage of Alstonia flowering in the first half of September, in this locality? Latitude & Longitude, please@Aditiya Joshi CUBE
Please coordinate with others @Aditiya Joshi CUBE
Yes @Aakanksha Ratnam College @Aanchal Chauhan Ratnam please let us collaborate and pull more people in this national meet even if we are not doing anything
[12:19 PM, 9/7/2021] Aditiya Joshi CUBE: So… We have them flowering in kolkata… Thanks @Batul Pipewala Kolkata
[12:26 PM, 9/7/2021] Aditiya Joshi CUBE: I too dont know actually why…
Maybe it can be poisonous… Maybe sap or bark or such…
Actually it is also called scholar’s tree or blackboard tree because its said that the wood of this tree was used to make boards n slates…
Even the scent of flowers… Its very strong… Here in evening it spreads nearby area of tree with winds… A sweet n spicy kind of smell… Some have breathing problem or headaches due to this…
@Raj Yashwantrao Patil CUBE
But m not sure too
I got this pdf. We can discuss if its reliable or good refrence
Thanks a lot!
Will surely read this!
[12:55 PM, 9/7/2021] Sunita Chahar CUBE: Alstonia had very good pollution absorbing index,so its chosen for avenue
[12:55 PM, 9/7/2021] Sunita Chahar CUBE: I have one near my house
[12:56 PM, 9/7/2021] Aditiya Joshi CUBE: Ohh… Pollution absorbing index…
[12:57 PM, 9/7/2021] Aditiya Joshi CUBE: Can you please explain this more ma’am. Its intresting …
Why stop doing things? Are you in an area deprived of biological systems? We ourselves are Biological System and our gadgets too are useful to address biological phenomena.
How about trees, plants food and animal around us? @Ishita Assam
How to take good photos to post?
How about your nails? How about Mango and other trees nearby?
How about mosquitoes and butterflies? Fruitflies? Our own food? @Ishita Assam
There’s an abundant of biological systems if we look around
I Will be posting the pictures I get from my area now
Also there is a flower in my room planted by my roommate that blooms every morning, it’s leaf closing and opening patterns are so clearly visibly
Which made me think why we aren’t talking about it too
[2:45 PM, 9/7/2021] Arunan HBSCE: I remember local people associate the aroma of flowers to witches. Interesting! Instead of addressing questions, we used to dismiss most natural phenomena, attributing to witches and devil’s!
[2:48 PM, 9/7/2021] Arunan HBSCE: Really, breathing problem and headaches? Is it another myth? @Aditiya Joshi CUBE
Or there is reason for it?

This is the plant that my roommate planted, it shows a very clear closing and opening of leaves and flowers

I will capture a Timelapse where the whole activity will be visible

It also seems to bend towards the sunlight more
Please tell us the time of opening and closing of flowers and leaves.
Interesting.
Any idea which plant is this?
Please give some description with close up photos. @Ishita Assam
Essential description like Objective, *Location, Date, Time and Name of Data collector a with each photo please @Ishita Assam
Any idea which plant is this? @Ishita Assam
Mango fruiting started Location Karkudi,Tenkasi, Tamilnadu 8.9831° N, 77.2075° E Date 7.9.2021 Nearly six trees starts flowering and fruiting Data collector Binumol &Shrudhiga
Six trees out of how many surveyed? @Dr. Binumol SN Nattika

Mango panicle Date 7.9.2021 Location Karkudi, Tenkasi, Tamilnadu 8.9831° N, 77.2075° E Data Collector Binumol &Shrudhiga
What do we mean by panicle? @Dr. Binumol SN Nattika
What percentage of Mango trees show flowering in this locality? @Dr. Binumol SN Nattika

A panicle is a much-branched inflorescence.The branches of a panicle are often racemes. A panicle may have determinate or indeterminate growth.

Objective: Potato Slice as Medium for Microbial plating. 20 July 2021 While Potato is ready and here is the set up CUBE HomeLab Bhulabhai Desai Road, South Mumbai Photo: mcarunan

Objective: Potato Slice as Medium for Microbial plating. 20 July 2021 Boiled Potato is sliced here is the set up getting ready, with Fermented coconut water in the bottle. CUBE HomeLab Bhulabhai Desai Road, South Mumbai Photo: mcarunan.

Objective: Potato Slice as Medium for Microbial plating. 20 July 2021 Here is the set up getting ready. Streaking of fermented coconut water is done using the knife edge. CUBE HomeLab Bhulabhai Desai Road, South Mumbai Photo: mcarunan

Objective: Potato Slice as Medium for Microbial plating. 2 nd Day, 21July 2021 After ~20 hrs of ’ plating’ fermented coconut water. *Observation of Growth of colonies CUBE HomeLab Bhulabhai Desai Road, South Mumbai Photo: mcarunan

Objective: Potato Slice as Medium for Microbial plating. 2 nd Day, 22July 2021 After ~24 hrs of ’ plating’ fermented coconut water. Observation of Growth of colonies CUBE HomeLab Bhulabhai Desai Road, South Mumbai Photo: mcarunan

Objective: Potato Slice as Medium for Microbial plating. 23July 2021 3rd Day of ’ plating’ fermented coconut water. Observation of Growth of colonies CUBE HomeLab Bhulabhai Desai Road, South Mumbai Photo: mcarunan
[4:25 PM, 9/7/2021] Aswathy Suresh CUBE: I missed that one fly , which are newly emerged one date of birth . After few days later I observed this newly emerged fly along with other transferred fly. It was 30th August 2021and next day I observed that fly were died inside bottle.
[4:32 PM, 9/7/2021] Aswathy Suresh CUBE: I posted this breaking news kind of updates in group alternative days but not checked these bottles continuously, that’s why I missed some important parts which are happened inside culture bottle.
[4:34 PM, 9/7/2021] Aswathy Suresh CUBE: Eggs I observed on 2nd onwards after transferred one fly… but larvae & pupae observed on 14th Day . 18th Day I observed new fly.
[4:39 PM, 9/7/2021] Aswathy Suresh CUBE: Iam nor much sure … because newly emerged flies are dieing very soon inside A culture bottle. That is the issue currently facing now.
[4:43 PM, 9/7/2021] Aswathy Suresh CUBE: Not*
[4:47 PM, 9/7/2021] Aswathy Suresh CUBE: I am planning to make media today 07.09.2021 and transferring flies on tomorrow 08.09.2021.
@Rechel Turkey Cube Ranchi Moina
Yes
[6:13 PM, 9/7/2021] Saida Sayyed: Yes
[6:13 PM, 9/7/2021] Saida Sayyed: Not able to join back
The page is reloading
Is there any issue in joining the CUBE chatShaala.

We can switch to jitsi.
Yes its taking too long to load
Yes. I find it difficult to join.
We can switch to jitsi . So that it would be easy for others to join.

I’ll share the link and also curcuilate it message in all the groups
Ok.
Attention!

Owing to unexpected technical issues regarding the regular Big Blue Button Platform where the CUBE Chatshaala is held everyday, we are shifting (temporarily, for today) to

Jitsi.

In case of any issues, please let us know in the group.
Isn’t it China rose?
[7:19 PM, 9/7/2021] Sunita Chahar CUBE: No, Portulaca
[7:26 PM, 9/7/2021] Sunita Chahar CUBE: View of ENDOPHYTIC FUNGI FROM PHYLLANTHUS AMARUS SCHUM. & THONN. CAPABLE OF PRODUCING PHYLLANTHIN, HYPOPHYLLANTHIN AND/OR RELATED COMPOUNDS | International Journal of Pharmacy and Pharmaceutical Sciences
Can we grow endophytes on TRSV medium

Yesterday I have made culture and today I can see that my earthworms are happy they are eating tissue papers. We can see that they are extracting so we can tell that earthworm can also lives in tissue paper. In soil earthworm eat cellulose they get cellulose from the leaves of tree which falls from tree and get decomposed in soil. So I think that tissue paper is made up of tree brak if the leaves of trees contain cellulose then the tree brak must contain cellulose and tissue paper is made up of tree brak then the tissue paper will also contain some amount of cellulose. So that’s why they can also lives in tissue paper
Can we grow plant pathogenic nematodes on TRSV medium?
Whiteboard Image of today’s(7th Sept 2021) Chatshaala Discussion👇🏼
Today we discussed about the work of @Naik Riddhi and @Seema Suthar on Fruitfly activity pattern

At what time we will get maximum and minimum no.of.flies?

What places we should keep the bait in order to attract flies?

Standardizing the design for studying the activity patter of Fruitflies!

Collaboration from CUBists in different parts of the Country on the activity patter study to present in the National Meet!

Looking forward for a short summary on these point from @Aswathy Suresh CUBE @Seema Suthar @Naik Riddhi @Rechel Turkey Cube Ranchi Moina
This image is fantastic @~Iram !
Please start with your objective everytime you post an update on your model system!
With the details like location, Cube locality lab, Date, Name of data collector!
These information are very crucial for maintaining records!
What’s your further plan?
Please follow up!
@~Iram
My further plan is that I will perform RER ( Rapid escape respons) in that I will learn about the regeneration of earthworm. Earthworm has a property that it can regenerate itself so I will see that how many days it will take to regenerate
Today we discussed about the work of @Naik Riddhi and @Seema Suthar on Fruitfly activity pattern

Today’s picture at 1148 hours
Which time you got maximum number of files @Naik Riddhi
430 pm
Ohh please tell in Detail @Naik Riddhi
[9:57 PM, 9/7/2021] +91 83790 10158: Today I observed fruit flies at different locations from 12 pm to 8 pm .
AIM TO OBSERVE THE NUMBER OF FLIES SEEN IN DIFFERENT PARTS OF HOUSE.
HYPOTHESIS - BALCONY TOMATOES WILL SHOW MAXIMUM FLIES
It was a rainy day.
Set 1- in kitchen where I kept fresh tomato and yesterday’s tomato too
Showed maximum number of flies during all 5 readings. The previous day’s tomato attracted more flies than fresh tomato
Set 2 in living room only fresh tomato kept
First showed 1 fly at 1400 hours then 6 at 1500 hours then 9 at 1630 hours then at 1900 hours 12 then 10 at 2000 hours
Set 3 on the balcony floor and set 4 in the balcony but at a higher level. Both fresh and previous day’s kept
Showed almost same results in 4/5 readings. At 1630 hours large diff were observed between set 3 and set 4. Large number of flies 16 were observed for set 3 while only 8 were observed for set 4. I think geotaxis might be the reason. Set 3 and set 4 the previous days tomatoes attracted negligible flies
THUS MY HYPOTHESIS WAS PROVED WRONG as maximum flies were seen in kitchen. I also feel artificial lights had a role as set 2 in tubelight Showed more flies than balcony set where it was dark post dusk
[9:57 PM, 9/7/2021] +9110158: Early morning at 800 hours contradicting results were seen
Maximum flies were seen in set 4 and minimum in set 1 and set 2. I feel the light intensity is responsible for these variations as inside it was darker than the balcony. Surprisingly more flies on the set at the higher level early in the morning than the lower level in contrast to my reading at 1630 hours.
[9:57 PM, 9/7/2021] +91 10158: At 945 hours Set 1 and set 2 didn’t show any significance diff. Set 3 and set 4 showed above 100 flies
[9:57 PM, 9/7/2021] +910158: This was observed yesterday
Hai Everyone,
We are planning do a small experiment , that across the country people can do it in their home lab. This experiment is related to fruit fly study. Every one can join with us.

1 Hour Experiment
4:30pm to 5:30pm

08.09.2021 _ (Today)

Question - if we keep a fruit (tomato/Banana) for trap fruit flies , which time we will definitely get flies (trapping/Culturing) in our area!?

Experiment is taking only 1 hour , we need to prove/disprove our hypothesis based on the observation that got by Seema and Riddhi and all other people who are done Cricadian Rhythm (activity pattern) experiment in fruit flies …

Seema’s (Goa) experiment : maximum number of flies at 5:00pm. Riddhi’s (Goa) experiment : maximum of flies at 4:30pm. Other collaborators also got maximum number of flies at around this time.

Hypothesis - In evening around 4:30pm to 5:30 time get ~25 flies (maximum number of flies) in one day .
So we are trying to developing a standard method of trapping fruit flies , Who can easily get flies from different parts of country at certain time.

Design of the experiment

︎3 empty plastic bottles (transparent)
︎Tomato pieces (1 tomato - cut in to small pieces)

We need to take 3 empty plastic bottle or glass bottle, make sure transparent bottle. 1 egg size tomato cut into small pieces and put into this three empty bottle. Location kitchen (were more number of flies can able to seen) Keeping this bottles three different parts of kitchen area .

︎Bottle 1 : Kitchen window side
︎Bottle 2 : Kitchen garbage bin area
︎Bottle 3 : Kitchen outside (Door side)

Experiment starting time 4:30 p.m. Keeping this for 1 hour After closing the bottle at 5:30pm. Then counting the number of fruit flies this three bottles.

Everyone please join (specially fruitflies collaborators) . This experiment we are planning to do it today…in order to prove/disprove this hypothesis.

@Arunan HBSCE @Naik Riddhi @Seema Suthar @Yash Sheregare CUBE @Man Masih Beck RANCHI CUBE @Sidhy Kerala Drosophila @Rechel Turkey Cube Ranchi Moina @Isha Pawle @Ishita Assam @Jyothika✨ @Shaharuban @Shrudhiga @Seethalakshmy CUBE @Lakshmy PJ CUBE @Arunima SN Nattika Cardamine @KIRAN CUBE @Lydia cUBe Drosphila @Batul Pipewala Kolkata @Dr. Nisy Prasad Cube @Janhvi Deshmukh @Raj Yashwantrao Patil CUBE @Abhijeet Singh

VIDEO SHARED BY DRISHTANT AT 6.44AM
Above is a video from a village area in the Osmanabad district of Maharashtra. On a bare field, two cricket enthusiasts are jugaadifying a method to erect three wooden sticks using some soil and whatever material is available around them to compensate for the wickets/stumps that we have in cricket.

CUBE runs on the same principle, I suppose!
Sophistication should be in the mind; not in fancy gadgets or in laboratory space.
~ Obaid Siddiqi

I also get reminded of these lines by poet Dushyant Kumar
‘हो कहीं भी आग, लेकिन आग जलनी चाहिए’ (Ho kahin bhi aag, lekin aag jalni chahiye)
English-- ‘For it doesn’t matter where, but this fire must burn’

This has been the story of CUBE throughout its decade-long journey especially in pandemic times.
So @Aswathy Suresh CUBE the reading has to be directly takn @5:30pm after the setup of experiment rt?
10 o’clock plant? @Sunita Chahar CUBE @Manasi CUBE
[9:12 AM, 9/8/2021] Manasi CUBE: Ohh what is the scientific name? Because I strongly think it looks like china rose… though china rose is the vernacular name. Does it flower?
[9:13 AM, 9/8/2021] Manasi CUBE: Ohh one more vernacular name! What say? @Sunita Chahar CUBE
@Arunan HBSCE
Interesting.
What’s your comment on this quote from the same article? @Sunita Chahar CUBE @Lakshmy PJ CUBE @Shraddha Sonavane CUBE

"Phyllanthin (a bitter constituent) and Hypophyllanthin (a non-bitter constituent) [21, 22] are the active principles responsible for the hepatoprotective role [23] of this plant and are present in combination only in P. amarus and not in the other related Phyllanthus species [24]. P. amarus with these twin “magic bullets” was chosen for the current study and endopthytic fungi from different parts were isolated and screened for their production in vitro."View of ENDOPHYTIC FUNGI FROM PHYLLANTHUS AMARUS SCHUM. & THONN. CAPABLE OF PRODUCING PHYLLANTHIN, HYPOPHYLLANTHIN AND/OR RELATED COMPOUNDS | International Journal of Pharmacy and Pharmaceutical Sciences
Wonderful😇 @~Iram of 10th Grade, Sir Sayyed School, Mankhurd, Mumbai for reviving Earthworm model system.
CONGRATULATIONS @~Iram for such a good photo and a very clear description.
Please add dates of your set up and Date of Observation in every photo of the post. Please repost this great photo, with dates, to all groups.
Looks like, you can see the feces of these Earthworms as black soil particles and also whitish or greyish fecal particles after eating white paper. @~Iram
Wonderful graph on the number of Fruitflies attracted to fruits at the CUBE HomeLab of @Seema Suthar Panjim, Goa
CONGRATULATIONS😇 @Seema Suthar
[9:35 AM, 9/8/2021] Arunan HBSCE: @Abhijeet Singh kindly give a short description on this for all to understand understand and to continue discussion.
[9:56 AM, 9/8/2021] Arunan HBSCE: Please describe the method of how we are planning to do studies on Rapid Escape Response of Earthworms? @~Iram
[9:58 AM, 9/8/2021] Arunan HBSCE: Wonderful How can we study Regeneration in Earthworms? @~Iram
Please give the plan of work.
Thank you Sir

Found another collaborator!

A snail brought home by my brother. I will send closeup photos in a while!

Snail collected at Mahad homelab, time 10:30 a.m.

wow it’s look to different …@Manasi CUBE
Yeah it has really long tentacles!
What is the name of this snail …do you have any idea @Manasi CUBE
No. I’m searching for the same

I tried searching using Google lens and this is what I found
I will perform RER (Rapid escape response). I will take 10 earthworm and 10 test tube of distilled water (water which has no minerals) and 10 test tube of NaCl solution

(For making NaCl solution I will take 2 beakers. In beaker 1 I will take 100 ml of distilled water. Then I will take 5.84 g of salt. In beaker 2 I will take again 100 ml of distilled water and put 5.84 g of salt. Take measuring cylinder. From beaker 1 I will take 80 ml of distilled water and from beaker 2 I will take 20 ml of NaCl water and mix it . Then put it in a conical flakes and cover it with cotton)

In 10 test I will put 2 ml of distilled water and in another 10 test tube I will put 2 ml of NaCl solution.

First I will put earthworm in distilled water and put the timer of 2 min if …
Yes the photo is very similar to the snail that I’ve found @Isha Pawle

First you dissolved 5.84 g salt in 100 mL distilled water,
then from this solution you took out 20 mL solution and dissolved it again in 80 mL distilled water.

It seems as if you are diluting the salt concentration.
If so, what is the need to do so?
I would also like to know about your collaborators who are into this excellent work.
Wow! Which class your brother is in? At Mahad? @Manasi CUBE
Looks like an aquatic snail, is it?
Is it fair to conclude an earthworm’s disability with just one Rapid Escape Response Test?
[1:17 PM, 9/8/2021] Arunan HBSCE: Where exactly your brother pick up this snail from, when? @Manasi CUBE
Let’s have a description of the habitat and also habit as you see it.
[1:17 PM, 9/8/2021] Arunan HBSCE: Different from which ones?.@Janhvi Deshmukh
[1:19 PM, 9/8/2021] Arunan HBSCE: What do you mean by tentacles? Don’t you expect tentacles in Giant African Snail? How different this is from the Giant African snails? @Manasi CUBE
Please give some description. Very interesting.
[1:21 PM, 9/8/2021] Arunan HBSCE: How do we search? Please give your method. Let others pitch in too. @Manasi CUBE @Janhvi Deshmukh @Anjani Kumar Kashyap CUBE
[1:21 PM, 9/8/2021] +91 81041 39244: If I will not dilute the NaCl solution and directly put earthworm in higher concentration of NaCl then earthworm may dye
[1:22 PM, 9/8/2021] +91 81041 39244: I am only doing this work
In case you do not have test tubes, can we still do Rapid Escape Response test? @~Iram @Man Masih Beck RANCHI CUBE @Abhijeet Singh

@Manasi CUBE Do you have Earthworms at Mahad, Maharashtra? @Manasi CUBE
My brother is in STD 8th presently.
Earthworm is plentily available in all parts of India.
So, why not invite Cubists to join you in this interesting experiment of Nerve Cord Regeneration? @~Iram
[1:30 PM, 9/8/2021] Manasi CUBE: I’m not sure. So I’m going to visit the place where he got the snail from to see the habitat and find more snails.
[1:31 PM, 9/8/2021] Manasi CUBE: I’m going to visit the habitat today and will update likewise.
Please start a CUBE Mahad group with your brother and his friends.

He can join also with @~Iram in Ventral Nerve Cord Regeneration studies using Mahad Earthworms.. @Manasi CUBE
Actually I uploaded the photo of my snail on Google lens and looked for results. Google lens shows its name as Channeled applesnail but there are a variety of snail photos under it. So we still have to search more
[1:33 PM, 9/8/2021] Arunan HBSCE: Can’t you engage your brother in a discussion before visiting

KiranKalakotiR · September 8, 2021, 8:59am

Till 8th September 2021, 9.30pm
that place from where he picked this interesting snail? @Manasi CUBE
[1:35 PM, 9/8/2021] Arunan HBSCE: Can’t you describe the features of this snail compared to that of Giant African Snail which you are familiar with?
Let’s have discussion to inspire more cubists
@Manasi CUBE
[1:37 PM, 9/8/2021] Arunan HBSCE: Good.
Please ask your brother to take long shot photos of the place from where he picked up and let’s have discussion. @Man Masih Beck RANCHI CUBE
“… earthworms may die” pretty good hypothesis
Can’t we do this experiment at home @~Iram?? Without test tubes, beakers and measuring cylinders, etc.
Very interesting challenge! Hope, others like @Janhvi Deshmukh @Aswathy Suresh CUBE @Arunima SN Nattika Cardamine @Aastha Ahuja Cube @Ishita Assam @Isha Pawle and others will pick up too. @Rechel Turkey Cube Ranchi Moina
[1:55 PM, 9/8/2021] Abhijeet Singh: Yes sir!
So if you follow this thread you’ll get the whiteboard Image of yesterday’s Chatshaala Discussion!

We were discussing about the activity pattern of Fruitflies in the different hours of the day!

So @Naik Riddhi and @Seema Suthar from Goa conducted an small experiment to check the pattern of Fruitflies.

As per Riddhi’s observation she got maximum no.of.flies at 4.30pm and minimum at 7.00pm.

As per Seema she got maximum number of Fruitflies at 5.00pm and minimum at 12.00AM(Midnight)

We were comparing these observations with @Aswathy Suresh CUBE 's observations through the experiment she did in August 2020.
Aswathy got maximum number of Fruitflies at 7.00PM and minimum number of Fruitflies at 12.00Am.

So after that we are planning to conduct a nationwide check for the activity patter of Fruitflies from 4.30-5.30pm. As Posted by @Aswathy Suresh CUBE in the group.

@Naik Riddhi @Seema Suthar please correct me if I’m wrong somewhere and please continue this discussion and your further plans! Looking forward!
[2:04 PM, 9/8/2021] Abhijeet Singh: Great work👏 @~Iram. How are you planning do conduct the RER test at your home lab. ?

Can you also please clarify the connection of RER with regeneration in Earthworms? How does RER helps you in order to achieve your short, long term objective
@~Iram ?
[1:55 PM, 9/8/2021] Abhijeet Singh: Yes sir!
So if you follow this thread you’ll get the whiteboard Image of yesterday’s Chatshaala Discussion!

We were discussing about the activity pattern of Fruitflies in the different hours of the day!

So @Naik Riddhi and @Seema Suthar from Goa conducted an small experiment to check the pattern of Fruitflies.

As per Riddhi’s observation she got maximum no.of.flies at 4.30pm and minimum at 7.00pm.

As per Seema she got maximum number of Fruitflies at 5.00pm and minimum at 12.00AM(Midnight)

We were comparing these observations with @Aswathy Suresh CUBE 's observations through the experiment she did in August 2020.
Aswathy got maximum number of Fruitflies at 7.00PM and minimum number of Fruitflies at 12.00Am.

So after that we are planning to conduct a nationwide check for the activity patter of Fruitflies from 4.30-5.30pm. As Posted by @Aswathy Suresh CUBE in the group.

@Naik Riddhi @Seema Suthar please correct me if I’m wrong somewhere and please continue this discussion and your further plans! Looking forward!
[2:04 PM, 9/8/2021] Abhijeet Singh: Great work👏 @~Iram. How are you planning do conduct the RER test at your home lab. ?

Can you also please clarify the connection of RER with regeneration in Earthworms? How does RER helps you in order to achieve your short, long term objective
@~Iram ?
They looks different from each other sir @Arunan HBSCE sir
@~Iram
We are all here to help you with your doubts and clarifications. Yours as well as ours! Let’s follow up on this!

Also lets discuss this in Chatshaala as well! Looking forward…!
Great to see you back Iram.
Can you send pictures of your earthworm cultures?
How many earthworms do you have?
How are you maintaining them?
How big these earthworms are to perform rapid escape response test?
Great @~Iram !what’s the situation today …you say that they are happy with tissue paper…but you add salt water so what the situation ?..can earthworm lives happily with salt water ?@~Iram
[2:17 PM, 9/8/2021] Arunan HBSCE: As Amitabh Bachchan, too looks different from me. @Janhvi Deshmukh
[2:20 PM, 9/8/2021] Arunan HBSCE: Whaaat? @Janhvi Deshmukh:face_with_raised_eyebrow:

Did @~Iram add salt to ater to these cup cultures?
No @Arunan HBSCE sir sorry I mix two things sorry @~Iram

KiranKalakotiR · September 14, 2021, 8:54am

8th September 2021, 9.30pm to 9th September 2021, 9.30pm
As many as 124 cases of dengue have been reported in Delhi between January 1 and September 4 — the highest since 2018, when the city saw 137 cases of the vector-borne disease. Of these total cases, 72 have been reported in August alone.

Delhi sees rise in dengue cases in the month of August

How is the rainfall in delhi NCR at present? @Raj Yashwantrao Patil CUBE @Aastha Ahuja Cube

what’s your comment on this?@Gaurangi Dhuri @Hina Mudgal Kanpur CUBE
[3:04 PM, 9/8/2021] Raj Yashwantrao Patil CUBE: The rainfall currently is abrupt and heavy!
[3:04 PM, 9/8/2021] Raj Yashwantrao Patil CUBE: In this week it has rained thrice in my area!
Please take care that water shouldn’t get collected in your locality!
Let’s try to Aware your locality of this possible breeding sites for mosquitoes! @Raj Yashwantrao Patil CUBE
SOUNDS GREAT!!
With another cubist’s curiosity, another interesting project regenerates @~Iram ! CHEERS!

So @~Iram what do you think must be common and different between snail’s nerve cord regeneration and earthworm’s body segment regeneration ???

What other cubist’s think?? Just to join the dots of knowledge at CUBE!
[3:26 PM, 9/8/2021] Arunan HBSCE: What do you expect? @Janhvi Deshmukh
[3:28 PM, 9/8/2021] Arunan HBSCE: How is mosquito menace in Delhi? @Aastha Ahuja Cube @Raj Yashwantrao Patil CUBE @P. Chitralekha Cube JNU and others
[3:29 PM, 9/8/2021] Arunan HBSCE: Please give some more details @Raj Yashwantrao Patil CUBE Which area? Any documentation by weather beureau? @Raj Yashwantrao Patil CUBE @Aastha Ahuja Cube
Rainfall is very common almost 3 times a week and sometimes more
[3:31 PM, 9/8/2021] Arunan HBSCE: No news from Delhi & Haryana Cubists on Mosquito borne diseases. Hope to hear on the initiatives.
[3:32 PM, 9/8/2021] Arunan HBSCE: Photos of possible breeding sites in your locality @Raj Yashwantrao Patil CUBE @Aastha Ahuja Cube @P. Chitralekha Cube JNU @Rahul Kushwaha and others
Sir i have not been regualr with newspapers quite a few week so I don’t know whats the status of diseases spread by mosquitioes.

But to say of my locality(B.K.S Marg, New Delhi-110001) I haven’t seen any mosquito insects at my home.
Any reference letters nk on rainfall in 2021 vs 2020 August & September? @Raj Yashwantrao Patil CUBE @Aastha Ahuja Cube and others.
Is there any major difference?
Can you tell how many mosquitos are present nowadays?
What is your hypothesis?
Can we start now to verify or falsify the hypothesis?
Reminder
8th September, 2021
We are heading towards what I assume to be the " India’s Largest Collaborative Homelab Experiment" to find out the Activity Pattern in Fruitflies where CUBists across India, from North to South, East to West, will participate at the same given time(4:30 pm to 5:30 pm).
Make sure you have:
-egg sized red tomato
-3 plastic bottles
-3 cotton plugs/bottle caps
-also your phone with a functioning camera
Any querries related to the experiment are most welcomed to be discussed asap since we are running out of time @Aswathy Suresh CUBE @Isha Pawle @Ishita Assam
[3:38 PM, 9/8/2021] Arunan HBSCE: Do you have a Design to estimate mosquitoes including possible mosquito breeding sites in your BKS Marg & respective localities? @Raj Yashwantrao Patil CUBE @Raj Yashwantrao Patil CUBE @P. Chitralekha Cube JNU @Rahul Kushwaha
[3:40 PM, 9/8/2021] Arunan HBSCE: In case, I do not have a bottle, can I use any other container as a trap? @Man Masih Beck RANCHI CUBE
In that case, transparent containers which could be covered(at 5:30 pm) to prevent flies from escaping could be used.
We have to cover, then click pictures and then count the number of flies after time out(5:30 pm)
Can you put the names on X and Y axis, so that it becomes more clear!
What is the story coming out of graph? Let’s discuss it…

You may go through this again.
Let’s Do this !

Protocol for Fruitfly activity Pattern Experiment

-Take an egg sized tomato
-cut this one tomato into pieces
-divide the pieces into 3 equal parts.
-Take 3 transparent plastic bottles(preferrable) of any capacity, tag them as
Bottle 1,
Bottle 2 and
Bottle 3
-Insert one divided tomato pieces into Bottle 1, another to Bottle 2 and yet another to Bottle 3
-Place them open at:(at 4:30 pm)
•Bottle 1 - Kitchen Window
•Bottle 2- Kitchen garbage
•Bottle 3- outside kitchen/kitchen door

At sharp 5:30 pm
-Cover the bottles using cotton plugs or caps
-take pictures, count the number of flies and share on different CUBE Groups

@Arunima SN Nattika Cardamine @Lakshmy PJ CUBE @Saida Sayyed @Aswathy Suresh CUBE @Aditiya Joshi CUBE @Abhijeet Singh @Raj Yashwantrao Patil CUBE @Sachin Pradhan CUBE and others

VIDEOS SHARED BY SIDHY AT 4.10PM
Transferred ~20 flies from SLTT-H to SLTT-H1 and ~15 flies from SLTT-I to SLTT -I1. Date: 04/09/2021 , Time: 11:45 PM. Location: Thanniam ,Thrissur ,Kerala. Sidhy.P.P , CUBE S N College, Nattika.
Single line culture of fruit flies.

SLTT-H1
Transferred ~20 flies from SLTT-H on 04/09/2021. TRSV medium was prepared on 03/09/2021

Observation on 08/09/2021 (4 th day):
~20 flies , ~ 40 larvae , 2 pupae

Location: Thanniam, Thrissur,Kerala.

Sidhy.P.P , CUBE S N College,Nattika.
Single line culture of fruit flies.

SLTT- I1
Transferred~15 flies from SLTT-I on 04/09/2021. TRSV medium prepared on 03/09/2021.

Observation on 08/09/2021(4 th day):

~ 13 flies , 5 larvae , 3 eggs
Condensation inside the bottle.
Medium became semisolid.

Location: Thanniam, Thrissur,Kerala.

Sidhy.P.P , CUBE S N College,Nattika.
Single line culture of fruit flies.

SLTT-H2
Transferred 2 flies from SLTT-H on 06/09/2021.
TRSV medium prepared on 03/09/2021.

Observation on 08/09/2021(2 nd day):
2 flies , ~ 10 small larvae .

Location: Thanniam, Thrissur, Kerala.

Sidhy.P.P , CUBE S N College, Nattika.
Congratulations @Sidhy Kerala Drosophila!
Reminding everyone @Man Masih Beck RANCHI CUBE @Naik Riddhi @Yash Sheregare CUBE @Ishita Assam @Isha Pawle @Batul Pipewala Kolkata @@KIRAN CUBE @Seema Suthar @Seethalakshmy CUBE
Thanks @Man Masih Beck RANCHI CUBE

Fruitflies Activity Pattern I hope everyone is ready with a chopped tomato

Yes we are ready @Man Masih Beck RANCHI CUBE
Please write objective of the experiment @Sidhy Kerala Drosophila All essential description for all others to follow, please!
1 pc in 1 bottle rt
Depends on you @Seema Suthar do you want to add two three piece you can add
My conclusion is that the place where the flies are already present will show maximum flies initially but eventually flies will be seen Maximum outside the house.
Great

Yes okay

Objective please, so that even fresh cubists can understand and become your collaborators. @Sidhy Kerala Drosophila

Fruitfly Activity Pattern Few minutes more, and the odor molecules from the tomato pieces will be released
Fruitfly Activity Pattern
Bottles are placed at their respective places- Kitchen Window, Kitchen dustbin and outside Kitchen.

Experiment

Bottle 1 : Kitchen Window side.
Date : 08.09.2021 Time : 4:30pm

Objective : 3 bottles 4:30pm at different area of kitchen in order to find with in one hour how many flies will get each 3 bottle .

Data collector Aswathy Suresh CUB

Bottle 2 : Kitchen garbage bin area
Date : 08.09.2021 Time : 4:30pm

Objective : 3 bottles 4:30pm at different area of kitchen in order to find with in one hour how many flies will get each 3 bottle .

Data collector Aswathy Suresh CUBE

Bottle 3 : Kitchen outdoor area
Date : 08.09.2021 Time : 4:30pm

Objective : 3 bottles 4:30pm at different area of kitchen in order to find with in one hour how many flies will get each 3 bottle .

Data collector Aswathy Suresh CUBE

Fruitfly Activity pattern janhvi deshmukh homelab bajaj collage of science wardha
Ooh… here in Mumbai… till now I didn’t got any aedes mosquito… after I started catching mosquitoes again

Fruitfly second Activity pattern. … location:Wardha. Janhvi deshmukh homelab bajaj collage of science wardha
Great!

Following you guys! Have a great time @Man Masih Beck RANCHI CUBE and collaborators.

Bait kept near the one bottle near the kitchen window… janhvi deshmukh homelab bajaj collage of science wardha…Date:08/09/2021

Second bait kept basing area in the kitchen… janhvi deshmukh homelab bajaj collage of science wardha Maharashtra
3 rd bottle kept outside of kitchen…

. location:wardha Maharashtra janhvi deshmukh homelab bajaj collage of science wardha

Tomato

bait kept in 3 bottles.

Rechel’s CUBE Homelab morabadi Ranchi

Date: 08/09/2021

Objective : to find the number of trapped fruit flies in an hour.

Cut tomatoes into pieces and put them in 3 different bottles. Kept bottles in 3 different places ie kitchen garbage, window and outside the kitchen.

Bottle 1 - Near kitchen garbage bin. Date:8/9/2021
Time:4:30pm
Place:Mampilly
Fruitflies Activity pattern studies
Isha Pawle Homelab,
Kolhapur, Maharashtra.

3 open baits places in different places in kitchen area

Bait 1 - Near vegetable Basket

Bait 2- Near Kitchen washing Sink

Bait 3 - Near Kitchen garbage bag

Bottled -2 Near Kitchen window side. Date:8/9/2021
Time:4:40pm
Place:Mampilly
Bottle-3 -Outside kitchen, Date:8/9/2021
Time:4:30pm
Place:MampillyDescription please @Seethalakshmy CUBE
@Sarvada CUBE Goa and @Yuga from MHSS CUBE GOA … who are collaborating in the Fruitfly Activity today. It’s great that we are getting collaborators from different parts of the country.

Fruit fly Activity pattern Studies - Location - Prabhadevi, Mumbai

Location of traps

Kitchen Garbage bin
Kitchen window
Near Fruit basket
Time - 4:40pm
Can you make a summary of all the 4 patterns visible in the graph?
Let’s explain the graph to make it more clear to everyone…
image928×1600 135 KB

Bottle 1 : Kitchen window area
Date : 08.09.2021 Time : 4:30pm

Objective : 3 bottles 4:30pm at different area of kitchen in order to find with in one hour how many flies will get each 3 bottle .

Data collector Yuga Prabhudesai MHSS CUBE GOA

Bottle 1 : Kitchen outdoor area
Date : 08.09.2021 Time : 4:30pm

Objective : 3 bottles 4:30pm at different area of kitchen in order to find with in one hour how many flies will get each 3 bottle .

Data collector Sarvada Sail MHSS CUBE GOA

Bottle 2 : Kitchen garbage area
Date : 08.09.2021 Time : 4:30pm

Objective : 3 bottles 4:30pm at different area of kitchen in order to find with in one hour how many flies will get each 3 bottle .

Data collector Sarvada Sail MHSS CUBE GOA

Bottle 3: Kitchen window area
Date : 08.09.2021 Time : 4:30pm

Objective : 3 bottles 4:30pm at different area of kitchen in order to find with in one hour how many flies will get each 3 bottle .

Data collector Sarvada Sail MHSS CUBE GOA

All India Fruitfly Trap CUBE Mela, 8 Sept.2021, Trap 1, balcony1, CUBE HomeLab Bhulabhai Desai Road, South Mumbai Photo:mcarunan
Today I observed fruit flies at different locations from 12 pm to 8 pm .
AIM TO OBSERVE THE NUMBER OF FLIES SEEN IN DIFFERENT PARTS OF HOUSE.
HYPOTHESIS - BALCONY TOMATOES WILL SHOW MAXIMUM FLIES
It was a rainy day.
Set 1- in kitchen where I kept fresh tomato and yesterday’s tomato too
Showed maximum number of flies during all 5 readings. The previous day’s tomato attracted more flies than fresh tomato
Set 2 in living room only fresh tomato kept
First showed 1 fly at 1400 hours then 6 at 1500 hours then 9 at 1630 hours then at 1900 hours 12 then 10 at 2000 hours
Set 3 on the balcony floor and set 4 in the balcony but at a higher level. Both fresh and previous day’s kept
Showed almost same results in 4/5 readings. At 1630 hours large diff were observed between set 3 and set 4. Large number of flies 16 were observed for set 3 while only 8 were observed for set 4. I think geotaxis might be the reason. Set 3 and set 4 the previous days tomatoes attracted negligible flies
THUS MY HYPOTHESIS WAS PROVED WRONG as maximum flies were seen in kitchen. I also feel artificial lights had a role as set 2 in tubelight Showed more flies than balcony set where it was dark post dusk

CUBE HOMELAB KOLKATA Fruitfly bait kept at 4.30pm 1)near kitchen dustbin2)near kitchen window3)in the corridor8/9/21 BatulP
Bottle 2: Kitchen garbage area
Date : 08.09.2021 Time : 4:30pm

Objective : 3 bottles 4:30pm at different area of kitchen in order to find with in one hour how many flies will get each 3 bottle .

Data collector Yuga Prabhudesai MHSS CUBE GOA

1). Near the kitchen window
2). Balcony.
3). Under the vegetable basket .
Time: 4:30pm
Date:8/9/21
Location : Pazhuvil.
Data collector: Seethalakshmi
Bottle 3 : Kitchen outdoor area
Date : 08.09.2021 Time : 4:30pm

Objective : 3 bottles 4:30pm at different area of kitchen in order to find with in one hour how many flies will get each 3 bottle .

Data collector Yuga Prabhudesai MHSS CUBE GOA

Bottle 1 at Kitchen window side. 4:30 PM , 08/09/2021

Location : Thanniam , Thrissur , Kerala

Objective: To know how many flies can be trapped from kitchen window side this evening 4:30 to 5:30 PM.

By Sidhy.P.P , CUBE S N College, Nattika.

Bottle 1: Kitchen window area
Date : 08.09.2021 Time : 4:30pm

Objective : 3 bottles 4:30pm at different area of kitchen in order to find with in one hour how many flies will get each 3 bottle .

Data collector Sarvada Sail MHSS CUBE GOA

Bottle 2: Kitchen garbage area
Date : 08.09.2021 Time : 4:30pm

Objective : 3 bottles 4:30pm at different area of kitchen in order to find with in one hour how many flies will get each 3 bottle .

Data collector Sarvada Sail MHSS CUBE GOA

Bottle 2 near Kitchen garbage bin.4:30 PM , 08/09/2021

Location : Thanniam , Thrissur , Kerala

Objective: To know how many flies can be trapped from near kitchen garbage bin this evening 4:30 to 5:30 PM.

By Sidhy.P.P , CUBE S N College, Nattika.

Bottle 3 : Kitchen outside area
Date : 08.09.2021 Time : 4:30pm

Objective : 3 bottles 4:30pm at different area of kitchen in order to find with in one hour how many flies will get each 3 bottle .

Data collector Sarvada Sail MHSS CUBE GOA

Bottle near Kitchen door.4:30 PM , 08/09/2021

Location : Thanniam , Thrissur , Kerala

Objective: To know how many flies can be trapped from kitchen door side this evening 4:30 to 5:30 PM.

By Sidhy.P.P , CUBE S N College, Nattika.
Rahul: good to see the baits set!! waiting for nobel prize winning model system now!1

Trap 2 Balcony 2, 4:30 pm, 8 Sept.2021, All India Fruitfly Trap CUBE Mela, CUBE HomeLab Bhulabhai Desai Road, South Mumbai. Photo:mcarunan
Yes we can do instead of test tube I am going to use plastic bottles and instead of distilled water I am going to use normal tap water
Observation
Flies were seen around the bottle and on the bottle neck but no flies were seen inside the bottle on the bait.
Location porvorim goa by riddhi
Some 5-6 Fruitflies seen near the bottle 1 which was kept near kitchen garbage but not inside the bottle
Results - no Fruitflies observed inside the bottle but found a very few like 1 or 2 around the bottle near the kitchen garbage area.
Date : 08.09.2021 Time : 5:30pm

Objective : 3 bottles 4:30pm at different area of kitchen in order to find with in one hour how many flies will get each 3 bottle .

Data collector Sarvada Sail MHSS CUBE GOA
[5:39 PM, 9/8/2021] +91 88: Someone pls send the link for todays chatshaala
[5:40 PM, 9/8/2021] +91 \8: No I didn’t wipe the bottle neck.

Experiment : Result
Bottle 2 : Kitchen Garbagebin.
Date : 08.09.2021 Time : 5:30pm

Objective : With one hour 4:30pm to 5:30pm how many flies will get I each 3 bottles which are keeping different parts of kitchen area.

Close the bottle with cotton plug at 5:30pm.

Observation: 0 flies.

Data collector: Aswathy Suresh CUBE SN Nattika
Home Lab : Bottukadavu Thrissur Kerala.
What do you all say @Aswathy Suresh CUBE @Seema Suthar @Man Masih Beck RANCHI CUBE @Janhvi Deshmukh and others ??

Experiment : Result
Bottle 3 : Kitchen outdoor.
Date : 08.09.2021 Time : 5:30pm

Objective : With one hour 4:30pm to 5:30pm how many flies will get I each 3 bottles which are keeping different parts of kitchen area.

Close the bottle with cotton plug at 5:30pm.

Observation: 0 flies.

Data collector: Aswathy Suresh CUBE SN Nattika
Home Lab : Bottukadavu Thrissur Kerala.
Observation
5-6 fruitflies were seen in kitchen area and one fruitfly was trapped in the bottle which was kept near garbage area.
Data collector Yuga Prabhudesai MHSS CUBE GOA

maybe giving another one hour would be appreciable by the fruitflies
I didn’t get any flies
Yess
Result:no fruitfly observed inside bottle
Date :08/09/2021 time:5.30 pm
Objective :kept 3 bottle at different areas of kitchen in order to find with in one hour how many fruitfly will get in each bottle.
Data collector: janhvi deshmukh homelab bajaj collage of science wardha Maharashtra
In 3 bottles (Didn’t get any flies)
Fruit fly collaborators
Let’s have a discussion in CUBE chatShaala
No Aswathy
FRUITFLY activity pattern
Isha Pawle Home lab
Kolhapur Maharashtra,

Observations
8th September 2021
5:30 pm
Bait 1- (near vegetables basket )One fruit fly
Bait 2- (near kitchen washing Sink) One fruit fly
Bait 3- (Near Kitchen garbage bag) No fruitfly

KiranKalakotiR · October 22, 2021, 8:39am

9th September 2021, 9.30pm to 10th September 2021, 9.30pm

KiranKalakotiR · October 22, 2021, 8:40am

10th September 2021, 9.30pm to 11th September 2021 9.30pm