Effect of Clarithromycin (an antibiotic) in Cardamine

Effect of Clarithromycin (an antibiotic) in Cardamine.

Objective: To check the seed germination of Cardamine in different concentrations of Clarithromycin.

Hypothesis: As the concentration increases, seedlings will show yellow discoloration and a decrease in shoot and root length.
Setup:
Six bottles are labeled C, T1, T2, T3, T4, and T5, along with a stock.
Stock solution: 500 mg/L
C bottle: Distilled water.
T1 bottle: 10 mg/L
T2 bottle: 30 mg/L
T3 bottle: 50 mg/L
T4 bottle: 70 mg/L
T5 bottle: 90 mg/L
Twelve plastic cups are used, six of which are replicates.
Six droppers for each the concentration.

Place: Cherukkad, Kozhikode
Date:29/02/2024
Time:7pm
Seeds:
Twenty seeds are placed in each cup& a total of 240 seeds.
Seed collection: January.
Color of seed : Yellow.
Source of Cardamine: Delhi, collected during a meet-up with @shalinisharma98 .

Procedure:

1) Collect 7 bottles and 12 cups for the setup.
Source: Bottles are from my home and cups are collected from my cousin’s house.
2) Labeling and naming of cups, bottles, and dropper.
3) seed
Seed collection: January.
Color of seed : Yellow.
Total :240seeds
4) fill the cups with tissue paper and add 20 seeds to each cup.
5) Making distilled water.
6) Prepare different concentrations from the stock solution and add them to the corresponding bottles.
Place:Cherukkad, Kozhikode
Date:28/02/2024 &29/02/2024.
Theertha.

Step1
1)Collect 7 bottles and 12 cups for the setup.
Source: Bottles are from my home, and cups are collected from my cousin’s house.
Date:28/02/2024
Time:2pm
Place: Cherukkad, Kozhikode
Theertha


Step :2
2)Labeling and naming of cups, bottles, and dropper.
Date:29/02/2024
Time:11am
Place: Cherukkad, Kozhikode
Theertha

*Step:3)seed
Seed collection: January.
Color of seed : Yellow.
Total :240seeds.
Date:29/02/2024
Time:12.40pm
Place: Cherukkad, Kozhikode
Theertha


Step:4
4)fill the cups with tissue paper and add 20 seeds to each cup.
Date:29/02/2024
Time:5pm
Place: Cherukkad, Kozhikode
Theertha


Step:5
5)Making distilled water.
Date:29/02/2024
Time:12pm
Place: Cherukkad, Kozhikode
Theertha


Step:6
6)Prepare different concentrations from the stock solution and add them to the corresponding bottles.
Place:Cherukkad, Kozhikode
Date:29/02/2024
Time:5pm
Theertha.

Close photos of the setup :


C1,SEEDS ARE TREATED WITH DISTILLED WATER


C1R, REPLICATE OF CONTROL


T1
10mg/L


T1R, REPLICATE OF T1


T2,30mg/L


T2R, Replicate of T2


T3,50mg/L


T3R, Replicate of T3


T4 , 70mg/L


T4R, REPLICATE OF T4


T5,90mg/L


T5R, REPLICATE OF T5

How to make distilled water in homelab:

How to make distilled water in home ?
I found this video ,but why she is adding a dish Inside the pot ?@⁨shalini CUBE⁩ @⁨Chithra Mam⁩ @⁨~Smiti⁩ . Can I follow this method to make distilled water ? @⁨Chithra Ma’am⁩ @⁨Abhijeet CUBE⁩ & others .

Distilled water is water that has been boiled into vapor and condensed back into liquid in a separate container. Impurities in the original water that do not boil below or near the boiling point of water remain in the original container. Thus, distilled water is a type of purified water.

[29/02, 1:07 pm] Smiti: Yes you can follow this method to prepare distilled water at home. But why do you need distilled water @⁨Theertha⁩ ?

Is it to dissolve clarithromycin? I made distilled water at home in 2022 while I used to culture fruit flies… To make 20% sucrose solution.

Video by @Smiti .

![IMG_20240229_121818|375x499]

(upload://8py4CJL9TQsWFcRLJlotUgjxNWU.jpeg)
![IMG_20240229_121834|375x499]

(upload://2gILJQlLcqtcpbHRK7fbZxkdeG8.jpeg)
![IMG_20240229_121848|375x499]

(upload://jT5rHdTc0TT2c6JYYOW9BauBhhk.jpeg)

Thank you @⁨~Smiti⁩
Look what I made with your help! :smiley: in Kerala.
Making distilled water in homelab:
I set up this outside my home.
I used a half-filled pot (~2L).
I placed a dish in it while boiling the water.
Then, I placed a glass on top of the pot.
Finally, I added ice cubes on top .
Time taken :30 minutes
CUBE HOMELAB, CHERUKKAD, KOZHIKODE

Seed germination study in Cardamine using Clarithromycin (an antibiotic).
Day:2
Control:1
Date of setup:29/02/2024
Time of setup:7pm
Date:1/03/2024
Time:11pm
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:2
Control:2
Date of setup:29/02/2024
Time of setup:7pm
Date:1/03/2024
Time:11pm
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:2
T1:Test 10mg/L
Date of setup:29/02/2024
Time of setup:7pm
Date:1/03/2024
Time:11pm
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:2
T1R:Test 10mg/L, Replicate.
Date of setup:29/02/2024
Time of setup:7pm
Date:1/03/2024
Time:11pm
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:2
T2:Test 30mg/L
Date of setup:29/02/2024
Time of setup:7pm
Date:1/03/2024
Time:11pm
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:2
T2R:Test 30mg/L, Replicate.
Date of setup:29/02/2024
Time of setup:7pm
Date:1/03/2024
Time:11pm
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:2
T3:Test 50mg/L,
Date of setup:29/02/2024
Time of setup:7pm
Date:1/03/2024
Time:11pm
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:2
T3R:Test 50mg/L, Replicate.
Date of setup:29/02/2024
Time of setup:7pm
Date:1/03/2024
Time:11pm
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:2
T4:Test 70mg/L
Date of setup:29/02/2024
Time of setup:7pm
Date:1/03/2024
Time:11pm
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:2
T4R:Test 70mg/L, Replicate.
Date of setup:29/02/2024
Time of setup:7pm
Date:1/03/2024
Time:11pm
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:2
T5:Test 90mg/L
Date of setup:29/02/2024
Time of setup:7pm
Date:1/03/2024
Time:11pm
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:2
T5R:Test 90mg/L, Replicate.
Date of setup:29/02/2024
Time of setup:7pm
Date:1/03/2024
Time:11pm
Place : Cherukkad, Kozhikode
Theertha

Day:3

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:3
Control
Date of setup:29/02/2024
Time of setup:7pm
Date:2/03/2024
Time:8am
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:3
Control 2
Date of setup:29/02/2024
Time of setup:7pm
Date:2/03/2024
Time:8am
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:3
Test 1
Date of setup:29/02/2024
Time of setup:7pm
Date:2/03/2024
Time:8am
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:3
T1R
Date of setup:29/02/2024
Time of setup:7pm
Date:2/03/2024
Time:8am
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:3
T2
Date of setup:29/02/2024
Time of setup:7pm
Date:2/03/2024
Time:8am
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:3
T2R
Date of setup:29/02/2024
Time of setup:7pm
Date:2/03/2024
Time:8am
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:3
T3
Date of setup:29/02/2024
Time of setup:7pm
Date:2/03/2024
Time:8am
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:3
T3R
Date of setup:29/02/2024
Time of setup:7pm
Date:2/03/2024
Time:8am
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:3
T4
Date of setup:29/02/2024
Time of setup:7pm
Date:2/03/2024
Time:8am
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:3
T4R
Date of setup:29/02/2024
Time of setup:7pm
Date:2/03/2024
Time:8am
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:3
T5
Date of setup:29/02/2024
Time of setup:7pm
Date:2/03/2024
Time:8am
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Day:3
T5R
Date of setup:29/02/2024
Time of setup:7pm
Date:2/03/2024
Time:8am
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Control:1,using water
Date of setup:29/02/2024
Time of setup:7pm
Date:5/03/2024
Time:4pm
Breaking news
Germination:8/20=40%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Control:2,using water
Date of setup:29/02/2024
Time of setup:7pm
Date:5/03/2024
Time:4pm
Breaking news
Germination:10/20=50%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Test:1
Date of setup:29/02/2024
Time of setup:7pm
Date:5/03/2024
Time:4pm
Breaking news
Test 1
10Mg/L
Germination:7/20=35%

Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Test:1
Date of setup:29/02/2024
Time of setup:7pm
Date:5/03/2024
Time:4pm
Breaking news
Test 1 replicate:
Germination:5/20=25%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic) TEST:2
Date of setup:29/02/2024
Time of setup:7pm
Date:5/03/2024
Time:4pm
Breaking news
Test2
30Mg/L
Germination:2/20=10%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:5/03/2024
Time:4pm
Breaking news
Test 2Replicate:
Germination=0
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:5/03/2024
Time:4pm
Breaking news
Test :3
50Mg/L
Germination=1/20=5%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:5/03/2024
Time:4pm
Breaking news
Test 3 Replicate
Germination:3/20=15%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:5/03/2024
Time:4pm
Breaking news
Test 4
70Mg/L
Germination:1/20=5%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:5/03/2024
Time:4pm
Breaking news
Test :4 Replicate
Germination:8/20=40%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:5/03/2024
Time:4pm
Breaking news
Test :5
Germination:0
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:5/03/2024
Time:4pm
Breaking news
Test :5 replicate
Germination:0
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:6/03/2024
Time:5pm
Breaking news
Control:1,using water
Germination:14/20=70%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:6/03/2024
Time:5pm
Breaking news
Control:2, using water
Germination:12/20=60%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:6/03/2024
Time:5pm
Breaking news
Test:1,
10mg/L
Germination:14/20=70%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:6/03/2024
Time:5pm
Breaking news
Test:1 replicate
10mg/L
Germination:5/20=25%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:6/03/2024
Time:5pm
Breaking news
Test:2
30mg/L
Germination:3/20=15%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:6/03/2024
Time:5pm
Breaking news
Test:2 replicate
30mg/L
Germination:0
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:6/03/2024
Time:5pm
Breaking news
Test 3
50mg/L
Germination:8/20=40%
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:6/03/2024
Time:5pm
Breaking news
Test 3 replicate
50mg/L
Germination:10/20=50%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:6/03/2024
Time:5pm
Breaking news
Test 4
70mg/L
Germination:9/20=45%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:6/03/2024
Time:5pm
Breaking news
Test4 replicate
70mg/L
Germination:11/20=55%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:6/03/2024
Time:5pm
Breaking news
Test 5
90mg/L
Germination:3/20=15%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:6/03/2024
Time:5pm
Breaking news
Test 5 replicate:
90mg/L
Germination:3/20=15%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024
Time of setup:7pm
Date:7/03/2024
Time:11pm
Breaking news
Control
*Germination:14/20:70%
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:7/03/2024
Time:11pm
Breaking news
Control 2
*Germination:15/20:75%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:7/03/2024
Time:11pm
Breaking news
TEST 1,10mg/L
*Germination:20/20:100%
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:7/03/2024
Time:11pm
Breaking news
TEST 1 replicate,10mg/L
*Germination:8/20:40%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:7/03/2024
Time:11pm
Breaking news
TEST 2,30mg/L
*Germination:4/20:20%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:7/03/2024
Time:11pm
Breaking news
TEST 2 replicate,30mg/L
*Germination:1/20:5%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:7/03/2024
Time:11pm
Breaking news
TEST 3,50mg/L
*Germination:11/20:55%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:7/03/2024
Time:11pm
Breaking news
TEST 3, replicate 50mg/L
*Germination:10/20:50%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:7/03/2024
Time:11pm
Breaking news
TEST 4,70mg/L
*Germination:16/20=80%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:7/03/2024
Time:11pm
Breaking news
TEST 4 replicate,70mg/L
*Germination:12/20=60%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:7/03/2024
Time:11pm
Breaking news
TEST 5,90mg/L
*Germination:5/20=25%
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:7/03/2024
Time:11pm
Breaking news
TEST 5,90mg/L
*Germination:2/20=10%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:8/03/2024
Time:11pm
Breaking news
Control
*Germination:14/20=70%
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:8/03/2024
Time:11pm
Breaking news
Control 2
*Germination:16/20=80%
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:8/03/2024
Time:11pm
Breaking news
TEST 1,30 mg/L
*Germination:20/20=100%
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:8/03/2024
Time:11pm
Breaking news
TEST 1 replicate,30 mg/L
*Germination:11/20=55%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:8/03/2024
Time:11pm
Breaking news
TEST 2,50 mg/L
*Germination:7/20=35%
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:8/03/2024
Time:11pm
Breaking news
TEST 2, replicate 50 mg/L
*Germination:4/20=20%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:8/03/2024
Time:11pm
Breaking news
TEST 3,50 mg/L
*Germination:15/20=75%
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:8/03/2024
Time:11pm
Breaking news
TEST 3 replicate,50 mg/L
*Germination:13/20=65%
Place : Cherukkad, Kozhikode
Theertha


Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:8/03/2024
Time:11pm
Breaking news
TEST 4,70 mg/L
*Germination:17/20=85%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:8/03/2024
Time:11pm
Breaking news
TEST 4 replicate,70 mg/L
*Germination:15/20=75%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:8/03/2024
Time:11pm
Breaking news
TEST 5,90 mg/L
*Germination:7/20=35%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:8/03/2024
Time:11pm
Breaking news
TEST 5 replicate,90 mg/L
*Germination:5/20=25%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:9/03/2024
Time:5pm
Breaking news
Control
*Germination:15/20=75%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:9/03/2024
Time:5pm
Breaking news
Control 2
*Germination:16/20=80%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:9/03/2024
Time:5pm
Breaking news
TEST 1 ,10mg/L
*Germination:20/20=100%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:9/03/2024
Time:5pm
Breaking news
TEST 1 replicate ,10mg/L
*Germination:14/20=70%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:9/03/2024
Time:5pm
Breaking news
TEST 2,30mg/L
*Germination:10/20=50%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:9/03/2024
Time:5pm
Breaking news
TEST 2, replicate 30mg/L
*Germination:6/20=30%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:9/03/2024
Time:5pm
Breaking news
TEST 3,50mg/L
*Germination:17/20=85%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:9/03/2024
Time:5pm
Breaking news
TEST 3 replicate,50mg/L
*Germination:14/20=70%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:9/03/2024
Time:5pm
Breaking news
TEST 4,70mg/L
*Germination:20/20=100%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:9/03/2024
Time:5pm
Breaking news
TEST 4 replicate,70mg/L
*Germination:18/20=90%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:9/03/2024
Time:5pm
Breaking news
TEST 5,90mg/L
*Germination:10/20=50%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)*
Date of setup:29/02/2024
Time of setup:7pm
Date:9/03/2024
Time:5pm
Breaking news
TEST 5,90mg/L
*Germination:6/20=30%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024.
Time:7pm
Date:10/03/2024
Time:12pm
Control:1
Germination:15/20=75%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024.
Time:7pm
Date:10/03/2024
Time:12pm
Control:2
Germination:16/20=80%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024.
Time:7pm
Date:10/03/2024
Time:12pm
TEST 1
10mg/L
Germination:20/20=100%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024.
Time:7pm
Date:10/03/2024
Time:12pm
TEST 1 replicate
10mg/L
Germination:14/20=70%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024.
Time:7pm
Date:10/03/2024
Time:12pm
TEST 2
30mg/L
Germination:10/20=50%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024.
Time:7pm
Date:10/03/2024
Time:12pm
TEST 2 replicate
30mg/L
Germination:6/20=30%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024.
Time:7pm
Date:10/03/2024
Time:12pm
TEST 3
50mg/L
Germination:17/20=85%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024.
Time:7pm
Date:10/03/2024
Time:12pm
TEST 3 replicate
50mg/L
Germination:14/20=70%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024.
Time:7pm
Date:10/03/2024
Time:12pm
TEST 4
70mg/L
Germination:20/20=100%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024.
Time:7pm
Date:10/03/2024
Time:12pm
TEST 4 replicate
70mg/L
Germination:18/20=90%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024.
Time:7pm
Date:10/03/2024
Time:12pm
TEST 5
90mg/L
Germination:11/20=55%
Place : Cherukkad, Kozhikode
Theertha

Seed germination study in Cardamine using Clarithromycin (an antibiotic)
Date of setup:29/02/2024.
Time:7pm
Date:10/03/2024
Time:12pm
TEST 5 replicate
90mg/L
Germination:6/20=30%
Place : Cherukkad, Kozhikode
Theertha

Summary of seed germination of Cardamine using clarithromycin:

Percentage of seed germination with respect to the date:

Length of Root and shoot in the 10th day of setup:

.

Excel Sheet:


Percentage of seed germination Vs Different concentration of clarithromycin.

Conclusion: Clarithromycin is not inhibiting the seed germination in cardamine .What do you all say @Arunan @shalinisharma98 @Chitralekha @KiranyadavR @Abhi0703 &others.


Concentration of Clarithromycin Vs Length of seedlings.
Different concentration of clarithromycin effect the Root and shoot lengths.

Photos taken in the 6th day of seed germination:

Seed germination in cardamine using clarithromycin:
Picture shows the seedling of cardamine taken from control ( treated with water )& different concentrations of clarithromycin.
Place:cherukkad, Kozhikode
Date:6/03/2024
Time :4pm
Theertha

Seed germination in cardamine using clarithromycin:
Picture shows the seedling of cardamine taken from control ( treated with water )& different concentrations of clarithromycin.
Place:cherukkad, Kozhikode
Date:6/03/2024
Time :4pm
Theertha.

Photos taken on the 10th day of setup:


Control:
Germination:15/20=75%
Date:10/03/2024
Time:12pm
Place : Cherukkad, Kozhikode
Theertha


TEST 1
10mg/L of clarithromycin
Germination:20/20=100%.
Date:10/03/2024
Time:12pm
Place : Cherukkad, Kozhikode
Theertha


TEST 2
30mg/L of clarithromycin
Germination:10/20=50%.
Date:10/03/2024
Time:12pm
Place : Cherukkad, Kozhikode
Theertha


TEST 3
50mg/L of clarithromycin
Germination:17/20=85%
Date:10/03/2024
Time:12pm
Place : Cherukkad, Kozhikode
Theertha


TEST 4
70mg/L of clarithromycin
Germination:20/20=100%
Date:10/03/2024
Time:12pm
Place : Cherukkad, Kozhikode
Theertha


TEST 5
90mg/L of clarithromycin
Germination:11/20=55%
Date:10/03/2024
Time:12pm
Place : Cherukkad, Kozhikode
Theertha