Cardamine , a tool for doing Genetic Engineering in homelabs

Cardamine as a tool for doing genetic engineering in homelab:How are you planning to collect the seeds of Cardamine that there in ANDC,Delhi? And what is your plan of work? @⁨Enas CUBE⁩ .@⁨Smiti⁩

What is our actual question :interrobang: are we checking that Cardamine is already resistant to antibiotics ? If not can we make a antibiotic resistant Cardamine in homelabs ?@⁨Smiti⁩ @⁨Himanshu Joshi⁩ @⁨Enas CUBE⁩ @⁨~Dhanraj⁩ @⁨Chithra Ma’am⁩ @⁨shalini CUBE⁩ @⁨Abhijeet CUBE⁩ &others

@⁨Smiti⁩ please reshare it .and document your Cardamine seed germination study in

Ia m doing because you Cubists are inspired me to do . @⁨Smiti⁩ @⁨Abhijeet CUBE⁩ @⁨Enas CUBE⁩ @⁨Lakshmy CUBE⁩ @⁨Chithra Ma’am⁩ &others . What is our expectations regarding the experimental setups? @⁨Smiti⁩ @⁨Enas CUBE⁩ @⁨Himanshu Joshi⁩ @⁨Chithra Ma’am⁩ @⁨Lakshmy CUBE⁩ & others.

Kanamycin resistance is one of the most frequently used selection markers for obtaining transgenic plants. The introduction of these transgenic plants into agricultural practice will cause the kanamycin resistance gene and the gene product to be present on a large scale. The desirability of this situation is analysed.

Chatshaala whiteboard on 22nd February.

@⁨Smiti⁩ can you tell us about your plan of work using cardamine. Where are we getting stuck? What all things you needed for your set-ups? How can we collaborate eachother to perform effect of Kanamycin or ampicillin in cardamine.@⁨Abhijeet CUBE⁩ @⁨Smiti⁩ @⁨Kashyap Sapekhati⁩ @⁨~Dhanraj⁩ @⁨Chithra Ma’am⁩ &others.

The mechanism of action of streptomycin is inhibition of protein synthesis of mycobacteria in the ribosome. Resistance emerges when mutations appear in genes encoding 16S rRNA and protein S12.

Tetracycline antibiotics are protein synthesis inhibitors.[22] They inhibit the initiation of translation in variety of ways by binding to the 30S ribosomal subunit, which is made up of 16S rRNA and 21 proteins. They inhibit the binding of aminoacyl-tRNA to the mRNA translation complex. Some studies have shown that tetracyclines may bind to both 16S and 23S rRNAs.

ribosomes of the RER are not permanently attached to the membrane. They constantly attach and detach to the membrane as needed for protein synthesis.

It is in the penicillin class of medications. Ampicillin was developed to overcome the issue of drug resistance and extend the antimicrobial coverage of penicillins. It is also resistant to acid so it can be administered orally.


Phases of Cardamine. Photo: susantatanti, Sonari, Assam.

Cardamine seed collect in my chaitanya herbal garden pamgarh data collected by Nidhi devdas

culture of cardamine plant in our home lab I observe Flowering and fruiting has started in cardamine plant
.photo :Anisha tandan .

Following summary by @enas

In the Chatshaala we disscussed about Cardamine.
• When Cardamine seeds gets matured?
• How to collect Cardamine seeds?
• How many days takes moong neans takes to germinate if we it in the water ?

Experimental setup :
TITLE : Which seed germinate first Cardamine or mustard?
• 4 cap of plastic bottle with 5 layer of tissue paper. In 2 caps 5 mustard seeds and other 2 caps with 5 Cardamine seeds each.
• Watering at 8am, 2 teaspoon water in each cap daily.
My Expectation : Cardamine and mustard seeds will germinate in 4 days ( reason: since moong beans germinates within 3 days)
Dhanraj’s Expectation : Cardamine and mustard seeds will germinate in 6 days.
Please give your opinion.@⁨Arunan sir ⁩ @⁨~Dhanraj⁩ @⁨Theertha⁩ @⁨Smiti⁩ @⁨Himanshu Joshi⁩ @⁨Chithra Ma’am⁩ mam @⁨Kiran Ma’am @⁨shalini CUBE⁩ @⁨~Ajita Banerjee⁩

Following summary made by @Enas

Discussion started with @⁨Smiti⁩ who is having Cardamine plant with her in her Homelab, Bandra, Mumbai and collected around 393 seeds out of it. The size of Cardamine is around 1 mm and it’s yellow to brown in colour.
Now. Plan is to study the effect of Kanamycin(antibiotic) on Cardamine plant.
What is the source of Kanamycin antibiotic?
:sparkles:Streptomyces kanamyceticus
What is meant by seed germination ?
• When we sow a seed, which part comes out first from the seed? And why?
:sparkles:Radicle comes first in almost all plants. Radical is the future root, which provide anchorage to the seedling and also absorbs nutrients from the soil which helps in the growth of the seedling.
What is seedling and sapling?@⁨Theertha⁩ @⁨Smiti⁩ @⁨Rahul CUBE⁩ @⁨Arunan sir :smiling_face:⁩ @⁨Chithra Ma’am⁩ mam @⁨~Aastha⁩

• What is the effect of any Penicillin or Kanamycin of bacterial cell? Do they kill the bacterial cell or retards the growth of bacteria ?
How Kanamycin will affect the Cardamine?
:sparkles:Kanamycin affect the 70s ribosome and particularly 30s subunit which ultimately affect or stops the protein synthesis present in Mitochondria and Chloroplast of Cardamine cell.

How Penicillin kills the bacteria?
:sparkles:The Penicillin inhibits the cell wall synthesis by acting on some enzyme.
Can you please elaborate more upon it?@⁨Smiti⁩

@⁨Arunan sir :smiling_face:⁩ @⁨Himanshu Joshi⁩ sir @⁨Theertha⁩ @⁨Chithra Ma’am⁩ mam @⁨~Ajita Banerjee⁩ mam

• What is the difference in plant cell wall and bacterial cell wall?
Will Cardamine pant show any effect if it is treated with Penicillin?@⁨Smiti⁩

@⁨Theertha⁩ can you please share your story of Cardamine?

Cardamine update : seed pods are formed. :smiling_face:
I will collect the seeds after it gets matured.
Further this seed can be used to study the seed germination in the presence of Kanamycin. @⁨Arunan sir :smiling_face:⁩ @⁨Chithra Ma’am⁩ mam @⁨Batul Ma’am⁩ mam @⁨~Urmi⁩ mam @⁨Theertha⁩ @⁨Abhijeet CUBE⁩ and others.
Date: 29th February 2024.
Place: Acharya Narendra Dev College, DU.
Name: Enas Shirin Fatma.

Cardamine seed collected from Acharya Narendra Dev College :partying_face::partying_face:
Date : 5th March 2024
Photo : Enas.