FLORAL DIP EXPERIMENT ON CARDAMINE, TO STUDY GENETIC ENGINEERING. ( Ranchi , 2021- 2022 Updates)

Floral dip method in cardamine plant.

  1. Growing cardamine plants :seedling::seedling:
  2. Culturing agrobacterium :microbe:
  3. Floral dip method in cardamine
  4. Collection of seeds
  5. Experiment with kanamycin to check the transformation

OBJECTIVE: To perform floral dip method in cardamine plant and check whether it is transformed or not.

  1. Growing cardamine plants :seedling::seedling:

Date: 2021-11-25T18:30:00Z
Objective : to plant cardamine saplings in cups to perform.

  1. Planted one small cardamine saplings with 4 to 5 leaves stage in 20 cups, containing garden soil in it.
  2. Prepared the soil cup on 23/11/2021.
  3. Transferred cardamine plants directly from the garden to each cup.
  4. Location: college, garden.
    Dr. Shyama Prasad Mukherjee University Ranchi.
  5. Watering cardamine plants once every day.

    Fig: Cardamine growing in the college garden, DSPMU, Ranchi.


Fig: Transferred cardamine plants into the cups.

Date:2021-12-03T18:30:00Z
Observation

  1. Added vermi compost in each cardamine saplings cup.
  2. No major development observed but plants are in good condition.

Pictures of developing cardamine saplings


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Fantastic. Can you help to explain why are you planning to perform floral dip method?
How are you planning to perform floral dip method?

1 Like

What is Floral Deep method?

1 Like