Major questions are addressed:
How to develop PH paper at Homelab
Reference shared:
Anthocyanins are flavonoid pigments synthesized in the cytoplasm and stored inside vacuoles. Many plant species accumulate densely packed, 3- to 10-μm diameter anthocyanin deposits called anthocyanin vacuolar inclusions (Ahttps://pmc.ncbi.nlm.nih.gov/articles/PMC4815043/#:~:text=Anthocyanins%20are%20flavonoid%20pigments%20synthesized,anthocyanin%20vacuolar%20inclusions%20(AVIs).
[02/04, 9:33 pm] Theertha: MANGO PRODUCTION IN AUSTRALIA
Authors
Ian S.E. Bally, P.R. Johnson, V.J. Kulkarni
Abstract
In Australia mangoes are grown in four States between the latitudes of 12° and 39° S. Production starts in the Northern Territory and Kimberley regions in early October, followed by the Queensland Dry Tropics in mid November, Mareeba in early December, Central Queensland in late December, and South East Queensland and Northern New South Wales in early January."
MANGO PRODUCTION IN AUSTRALIA.
Summary written by @KiranKalakotiR
[03/04, 10:36 am] Kiran : WHAT A COMPARISION OF 30° N OF BAGESHWAR UTTARAKHAND INDIA AND 30° S OF AUSTRALIA !!!
In yesterday’s chatShaala session we had the comparison of when do we get ripe local mangoes in 30 Degree North latitude of word map and apposite of it to 30 degree South of Word Map.
Bageshwar Uttarakhand In India is at 30 Degree North and local mangoes ripped in the month of May whereas, Australia is 30 Degree south and as per the reference shared during chatShaala ripped local mangoes can be found in the month of October which means it takes about half an year at these two locations.
What are your comment’s on this???…
Question addressed:Hot pan -Touch response
Can we do Plant genetic engineering in Homelabs ?
What are the requirements needed?@Rechel CUBE @Sailekshmi CUBE @ayanasudheer @~Kiran Kalakoti @Arunan sir @Chithra Ma’am and others
What is a Floral dip method?
Do it replace conventional Plant tissue culture?
@Rechel CUBE @ayanasudheer @@Sailekshmi CUBE and others
Reference were shared :
[03/04, 9:36 pm] Dhanraj CUBE: Cardamine at Metastudio Cardamine: Substitute Indian Model System for Arabidopsis
[03/04, 9:36 pm] Dhanraj CUBE: C. elegans
Isolation of soil Nematodes &looking for C.elegans in Indian soil
[03/04, 9:36 pm] Dhanraj CUBE: Review Curr Opin Genet Dev
. 2016 Aug:39:1-7. doi: 10.1016/j.gde.2016.05.005. Epub 2016 Jun 5.
Cardamine hirsuta: a comparative view
Angela Hay 1, Miltos Tsiantis 2https://pubmed.ncbi.nlm.nih.gov/27270046/
[03/04, 9:36 pm] Dhanraj CUBE: FLORAL DIP EXPERIMENT ON CARDAMINE, TO STUDY GENETIC ENGINEERING. ( Ranchi , 2021- 2022 Updates)
[03/04, 9:36 pm] Dhanraj CUBE: Fertilisation in plants
07 | May | 2011 | Anjung Sains Makmal 3
[03/04, 9:37 pm] Dhanraj CUBE: The embryo is an immature plant from which a new plant will grow under proper conditions. The embryo has onecotyledon or seed leaf in monocotyledons, two cotyledons in almost all dicotyledons and two or more in gymnosperms. The radicle is the embryonic root. The plumule is the embryonic shoot. TNAU Agritech Portal :: Seed Technology.
[03/04, 9:37 pm] Dhanraj CUBE: https://metastudio.org/uploads/default/original/2X/b/b76e4e1ccdea02f8432928a2056c8e8d050902f9.jpeg
[03/04, 9:37 pm] Dhanraj CUBE: Effect of Kanamycin in Cardamine and Chlorohydra
[03/04, 9:38 pm] Dhanraj CUBE: RNA-Seq Analysis of the Effect of Kanamycin and the ABC Transporter AtWBC19 on Arabidopsis thaliana Seedlings Reveals Changes in Metal Content
Plant responses to antibiotics have been poorly characterized. Nonetheless, several observations support the notion that chloroplasts are a major target of aminoglycosides. When plants are exposed to kanamycin, a characteristic symptom is the bleaching of the leaves indicating the breakdown of cholorophyll in choloroplasts [5]. When the chloroplast transporter MAR1 is mutated [6] or silenced [7], plants become resistant to kanamycin and several other aminoglycosides.
[03/04, 9:48 pm] Arunan sir : The Agrobacterium vacuum infiltration method has made
it possible to transform Arabidopsis thaliana without plant
tissue culture or regeneration. In the present study, this
method was evaluated and a substantially modified transformation method was developed. The labor-intensive
vacuum infiltration process was eliminated in favor of
containing Agrobacterium tumefaciens, 5% sucrose and
500 microliters per litre of surfactant Silwet L-77. Sucrose
and surfactant were critical to the success of the floral
dip method.``` Plants inoculated when numerous immature
floral buds and few siliques were present produced transformed progeny at the highest rate. https://abrc.osu.edu/uploads/document/document/2/CS16562_Transformation_Clough_and_Bent.pdf
What are the effects of Kanamycin Antibiotics in Cardamine?
Where it get effected ?
@<U+2068>~Batul Pipewala<U+2069> @<U+2068>Rechel CUBE<U+2069> @<U+2068>Chithra Ma'am<U+2069> and others
*Group photographs of today's chatshaala:4/04/2025*
[05/04, 9:46 pm] ayanasudheer: Whiteboard summary of today's chatshaala 05/04/25
[05/04, 9:46 pm] ayanasudheer: Whiteboard summary of today's chatshaala 05/04/25
[05/04, 9:47 pm] ayanasudheer: I never knew bacteria can be grown at home, that too by using potatoes as a medium!!! This is a TINKE moment for me🌻 Thank you @<U+2068>Enas CUBE<U+2069> @<U+2068>~RAHUL MALAKAR<U+2069> @<U+2068>~Kiran Kalakoti<U+2069> @<U+2068>Rechel CUBE<U+2069> for introducing this to me!
Whiteboard Summary of today's Chatshaala 06/04/25
Major questions are addressed:
1) How to isolate soil nematodes at Homelab
2)How to introduce setup to school students
Preparing for Exams
Questions addressed:
What is a neuron?
How does it function?
Response to hot pan :Reflex action
[07/04, 9:33 pm] Theertha: Hot chilli pepper
The starting point for the pair's ground-breaking discoveries was led by Julius' work and his fascination for natural products that probed biological function.
Julius studied the effect of capsaicin, the molecule that makes chili peppers spicy by simulating a false sensation of heat, to understand the skin's sense of temperature. Looking for the gene capable of reacting to capsaicin, he and his team created a library of millions of genes expressed in the sensory neurons which react to pain, heat, and touch. They then laboriously expressed these genes, one by one, in cells that couldn’t react to capsaicin, until they found one that did.https://www.understandinganimalresearch.org.uk/news/2021-nobel-prize-in-medicine-for-discovering-how-humans-feel-warmth-and-touch
[07/04, 9:33 pm] Theertha: Anatomy of Smell https://digfir-published.macmillanusa.com/gray7e/asset/img_ch7/c07_fig04.html
[07/04, 9:33 pm] Theertha: https://www.ncbi.nlm.nih.gov/core/lw/2.0/html/tileshop_pmc/tileshop_pmc_inline.html?title=Click%20on%20image%20to%20zoom&p=PMC3&id=3652637_nihms126639f1.jpg
[07/04, 9:35 pm] Arunan sir : https://digfir-published.macmillanusa.com/gray7e/asset/img_ch7/c07_fig04.html
[07/04, 9:38 pm] Arunan sir : Do we locate neuronal cell bodies in areas other than Brain and Spinal cord?
[08/04, 9:47 pm] Theertha: Whiteboard screenshot of today's chatshaala:8/04/2025
What are Parkinson flies ?
How to trap and culture Fruitflies in Homelab?
[08/04, 9:47 pm] Theertha: Whiteboard screenshot of today's chatshaala:8/04/2025
How does parkinson disease effect?
What are the parts of brain?
[08/04, 9:47 pm] Theertha: Whiteboard screenshot of today's chatshaala:8/04/2025
How to make BRSV Media?
@<U+2068>Akanksha CUBE<U+2069>
@<U+2068>~Batul Pipewala<U+2069> @<U+2068>~Kiran Kalakoti<U+2069> @<U+2068>Arunan sir <U+2069> @<U+2068>ayanasudheer<U+2069> @<U+2068>Dinesh Datta CUBE<U+2069> @abhinav @akashay @<U+2068>Babitha CUBE SN<U+2069> and others.
[08/04, 9:34 pm] Theertha: https://metastudio.org/t/fruitfly-culture-in-homelabs/15126
[08/04, 9:34 pm] Theertha: Parkinson's disease is a movement disorder of the nervous system that worsens over time. The nervous system is a network of nerve cells that controls many parts of the body, including movement.
Symptoms start slowly. The first symptom may be a barely noticeable tremor in just one hand or sometimes a foot or the jaw. Tremor is common in Parkinson's disease. But the disorder also may cause stiffness, slowing of movement and trouble with balance that raises the risk of falls.
In the early stages of Parkinson's disease, your face may show little or no expression. Your arms may not swing when you walk. Your speech may become soft or slurred. Symptoms get worse over time.
Although Parkinson's disease can't be cured, medicines may help symptoms get better. Sometimes a healthcare professional may suggest surgery to help control parts of the brain. This surgery may help lessen symptoms.
https://www.mayoclinic.org/diseases-conditions/parkinsons-disease/symptoms-causes/syc-20376055
[08/04, 9:44 pm] Theertha: *Reference shared in today's chatshaala*:8/04/2025
Questions are addressed:
How to find fruitflies in a kitchen?
Akshay took a photo and he think that was a fruit fly .What are the parts of a fruitfly ?
How to differentiate Fruitfly and beetles?
[09/04, 9:36 pm] Theertha: How fruit fly genes are helping scientists reverse Parkinson’s symptoms https://www.youtube.com/watch?v=DlPNeRuFZPQ
[09/04, 9:36 pm] Theertha: Fruit Flies Help Unlock Genetic Secrets of Parkinson's https://www.youtube.com/watch?v=wB6fnpC-WZ0
[09/04, 9:36 pm] Theertha: Beetle https://www.aces.edu/blog/topics/home/what-are-the-small-brown-beetles-in-my-home-drugstore-beetles/
[10/04, 10:23 pm] Theertha: What is the current status of mango flowering in Ranchi , Sapekshati and Bageshwar and other places?
@<U+2068>Rechel CUBE<U+2069> @<U+2068>~Kiran Kalakoti<U+2069> @<U+2068>Arunan sir <U+2069> @<U+2068>Enas CUBE<U+2069> and others
[10/04, 10:23 pm] Theertha: Comparing mango flowering status in 11°N and 8°N ,2022 and 2024 November.
Any comments @<U+2068>Rechel CUBE<U+2069> @<U+2068>Enas CUBE<U+2069> and others
[10/04, 10:23 pm] Theertha: What is the status of mango flowering in Ranchi ? ,any comparison @<U+2068>Rechel CUBE<U+2069> and others
[10/04, 10:23 pm] Theertha: What is the current mango flowering status at Ranchi,Tanda , Moradabad and other places !?
[10/04, 9:29 pm] Theertha: https://metastudio.org/t/100-mango-flowering-at-30-n-latitude-in-the-first-week-of-april-surveyed-total-72-mango-trees/15594?u=kirankalakotir 100% mango flowering at 30° N Latitude in the first week of April (Surveyed Total 72 Mango trees)
[10/04, 9:29 pm] Theertha: Varieties of Mangoes https://www.nhb.gov.in/report_files/mango/MANGO.htm#:~:text=Amrapalli%20(Dashehari%20x%20Neelum)%2C,grown%20commercially%20in%20the%20country).
[10/04, 9:29 pm] Theertha: https://indiangloriousnursery.com/shop/amrapali-mango-big-size/
[10/04, 9:29 pm] Theertha: Arunan MC Madel Goa
8:52 PM
Tanda › Coordinates
26.5433° N, 82.6645° E https://www.google.com/search?q=Tanda+Latitude&oq=Tanda+Latitude&gs_lcrp=EgZjaHJvbWUyBggAEEUYOTIICAEQABgWGB4yDQgCEAAYhgMYgAQYigUyDQgDEAAYhgMYgAQYigUyDQgEEAAYhgMYgAQYigUyDQgFEAAYhgMYgAQYigUyBwgGEAAY7wUyCggHEAAYgAQYogQyCggIEAAYgAQYogQyBwgJEAAY7wXSAQg1NDYxajBqN6gCALACAA&sourceid=chrome&ie=UTF-8
[11/04, 9:37 pm] Theertha: *Whiteboard screenshot of today's chatshaala:11/04/2025*
[11/04, 9:37 pm] Theertha: *Whiteboard screenshot of today's chatshaala:11/04/2025*
[11/04, 9:43 pm] Arunan sir : Researchers claim that fevers are more than just a symptom of illness or infection. They found that elevated body temperature sets in motion a series of mechanisms that regulate our immune system.
When we are healthy, our body temperatureTrusted Source tends to gravitate around 37°C (98.6°F).
But when faced with an infection or virus, body temperature often goes up, resulting in a fever.
When someone’s body temperature rises to about 38°C (100.4°F0, doctors classify it as a slight fever. Larger increases in body temperature to around 39.5°C (103.1°F) count as a high fever.
When a person has the flu, for instance, they may experience a mild and uncomfortable fever. This may drive many people to seek natural or over-the-counter remedies to treat it.
However, fevers are not always a bad sign. Mild fevers are a good indication that the immune system is doing its job. But fevers are not just a byproduct of the immune response.
In fact, an elevated body temperature triggers cellular mechanisms that ensure the immune system takes appropriate action against the offending virus or bacteria. https://www.medicalnewstoday.com/articles/321889
[11/04, 9:45 pm] Arunan sir : Any TINKE moments in today's Chatshaala? @<U+2068>Theertha<U+2069> @<U+2068>~Akhil Gupta Samuel<U+2069> @<U+2068>Enas CUBE<U+2069> @<U+2068>Himanshu Joshi<U+2069> @<U+2068>~Ajita Banerjee<U+2069> @<U+2068>Seethalakhmi<U+2069>
[12/04, 9:35 pm] Enas CUBE: What are different seasons? What is the difference between Climate, Seasons, and wheather?
12/04, 9:37 pm] Enas CUBE: What are different types of elections in India? How to measure the voters NAil ink Mark growth? What are the other alternative apart from Voters ink mark?
Whiteboard Screenshot of today's Chatshaala 13/04/25
Summary @Ayana_Sudheer
The next day of the experiment, at 8.14am 11 fruitflies were on the tomato slice!! What attracted the fruitflies to the tomato? Why did they choose tomato over banana? What are the compounds released from the tomato as they decay?
@<U+2068>~Akhil Gupta Samuel<U+2069> @<U+2068>Enas CUBE<U+2069> @<U+2068>Chithra Ma'am<U+2069> @<U+2068>Arunan sir @<U+2068>Susanta Tanti CUBE<U+2069>
Chatshaala discussions was about:
1) Butterfly and host plant
2) DNA extraction in College Vs Homelab
3)Cell division using onion root tip
Questions are addressed:
1)Food preferences in Fruitflies
2)Factors : Smell, Visions,Taste, Learning