Verification of Kanamycin gene transfer by growing cardamine seeds in MS media containing kanamycin.
After collecting seeds stored in a plastic zip lock bags. And labelled as P6, P7, P8, P9, P10 ( Here “P” stands for putative seeds as we are supposing that these have transformed) and C6, C7, C8, C9, C10 ( here “C” stands for Control cardamine plants.)
Picture was taken on 4th April, 2022 ( Dept of Microbiology, DSPMU RANCHI JHARKHAND)
Putative seeds ( from P10) were cultured on 14/02/2022 . In Murashige and skoog (MS) Media with kanamycin. Out of 10, 6 seeds are growing and elongating and two were stunt. Two didn’t germinate or not visible in this picture.
We can conclude that there were 10 seeds in total.
6 seeds grew in the presence of Kanamycin. Means 60% were transformed according to this sample size and 2 were able to grow but didn’t elongate. And another two was unable to grow.
Picture was taken on 4th April, 2022 ( Dept of Microbiology, DSPMU RANCHI JHARKHAND)
Normal seeds ( from C10) were cultured on 14/02/2022 . In Murashige and skoog (MS) Media with kanamycin. Out of 10, 5 seeds grew but remained stunt.
We can conclude that 50 % were able to germinate but didn’t elongate and the growth stoped. 5 seeds were not able to grow. ( According to the 10 seeds as sample)
Picture was taken on 4th April, 2022 ( Dept of Microbiology, DSPMU RANCHI JHARKHAND)
Putative seeds ( from P10) were cultured on 14/02/2022 . In Murashige and skoog (MS) Media with kanamycin. Out of 10, 2 seeds are growing and elongating and 4 were stunt.